Hideyoshi Harashima

Summary

Affiliation: Hokkaido University
Country: Japan

Publications

  1. pmc Quantitative analysis of condensation/decondensation status of pDNA in the nuclear sub-domains by QD-FRET
    Sharif M Shaheen
    Laboratory for Molecular Design of Pharmaceutics, Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo 060 0812, Japan
    Nucleic Acids Res 39:e48. 2011
  2. pmc Quantitative and mechanism-based investigation of post-nuclear delivery events between adenovirus and lipoplex
    Susumu Hama
    Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Hokkaido 060 0812, Japan
    Nucleic Acids Res 35:1533-43. 2007
  3. pmc The manner in which DNA is packaged with TFAM has an impact on transcription activation and inhibition
    Ryo Furukawa
    Laboratory for Molecular Design of Pharmaceutics, Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo 060 0812, Japan
    FEBS Open Bio 2:145-50. 2012
  4. pmc Characterization of a nudix hydrolase from Deinococcus radiodurans with a marked specificity for (deoxy)ribonucleoside 5'-diphosphates
    David I Fisher
    School of Biological Sciences, Biosciences Building, University of Liverpool, P, O, Box 147, Liverpool L69 7ZB, UK
    BMC Biochem 5:7. 2004
  5. ncbi request reprint Development of a novel systemic gene delivery system for cancer therapy with a tumor-specific cleavable PEG-lipid
    H Hatakeyama
    Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Hokkaido, Japan
    Gene Ther 14:68-77. 2007
  6. ncbi request reprint Pharmacokinetics of targeting with liposomes
    Hideyoshi Harashima
    Laboratory for Molecular Design of Pharmaceutics, Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Sapporo, Japan
    Crit Rev Ther Drug Carrier Syst 19:235-75. 2002
  7. doi request reprint A pH-sensitive fusogenic peptide facilitates endosomal escape and greatly enhances the gene silencing of siRNA-containing nanoparticles in vitro and in vivo
    Hiroto Hatakeyama
    Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo 060 0812, Japan
    J Control Release 139:127-32. 2009
  8. ncbi request reprint [Development of multifunctional envelope type artificial viral-like gene delivery system]
    Hideyoshi Harashima
    Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita 12 Nishi 6, Kita ku, Sapporo 060 0812, Japan
    Yakugaku Zasshi 127:1655-72. 2007
  9. ncbi request reprint Envelope-type lipid nanoparticles incorporating a short PEG-lipid conjugate for improved control of intracellular trafficking and transgene transcription
    Tomoya Masuda
    Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo, Hokkaido 060 0812, Japan
    Biomaterials 30:4806-14. 2009
  10. doi request reprint Intracellular fate of octaarginine-modified liposomes in polarized MDCK cells
    Takahiro Fujiwara
    Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo 060 0812, Japan
    Int J Pharm 386:122-30. 2010

Detail Information

Publications121 found, 100 shown here

  1. pmc Quantitative analysis of condensation/decondensation status of pDNA in the nuclear sub-domains by QD-FRET
    Sharif M Shaheen
    Laboratory for Molecular Design of Pharmaceutics, Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo 060 0812, Japan
    Nucleic Acids Res 39:e48. 2011
    ..This represents the first demonstration of the successful control of condensation/decondensation in specific nuclear sub-domain via the use of an artificial DNA condenser...
  2. pmc Quantitative and mechanism-based investigation of post-nuclear delivery events between adenovirus and lipoplex
    Susumu Hama
    Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Hokkaido 060 0812, Japan
    Nucleic Acids Res 35:1533-43. 2007
    ..Collectively, an improvement in nuclear decondensation and the diminution of the interaction between vector and mRNA is essential for the development of new generations of non-viral vectors...
  3. pmc The manner in which DNA is packaged with TFAM has an impact on transcription activation and inhibition
    Ryo Furukawa
    Laboratory for Molecular Design of Pharmaceutics, Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo 060 0812, Japan
    FEBS Open Bio 2:145-50. 2012
    ..This result indicates that TFAM can be useful packaging protein for exogenous DNA to achieve mitochondrial transgene expression...
  4. pmc Characterization of a nudix hydrolase from Deinococcus radiodurans with a marked specificity for (deoxy)ribonucleoside 5'-diphosphates
    David I Fisher
    School of Biological Sciences, Biosciences Building, University of Liverpool, P, O, Box 147, Liverpool L69 7ZB, UK
    BMC Biochem 5:7. 2004
    ..Characterisation of these hydrolases is necessary to understand the reason for their presence. Here, we report the cloning and characterisation of the DR0975 gene product, a nudix hydrolase that appears to be unique to this organism...
  5. ncbi request reprint Development of a novel systemic gene delivery system for cancer therapy with a tumor-specific cleavable PEG-lipid
    H Hatakeyama
    Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Hokkaido, Japan
    Gene Ther 14:68-77. 2007
    ..Thus, MEND modified with PPD is a promising device, which has the potential to make in vivo cancer gene therapy achievable...
  6. ncbi request reprint Pharmacokinetics of targeting with liposomes
    Hideyoshi Harashima
    Laboratory for Molecular Design of Pharmaceutics, Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Sapporo, Japan
    Crit Rev Ther Drug Carrier Syst 19:235-75. 2002
    ..Advanced technology for ligand-mediated selective targeting and intracellular targeting is also introduced for antitumor agents and for gene delivery systems...
  7. doi request reprint A pH-sensitive fusogenic peptide facilitates endosomal escape and greatly enhances the gene silencing of siRNA-containing nanoparticles in vitro and in vivo
    Hiroto Hatakeyama
    Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo 060 0812, Japan
    J Control Release 139:127-32. 2009
    ..Collectively, data demonstrate that introduction of both of a pH-sensitive fusogenic GALA peptide and PPD into the MEND facilitates nanoparticle endosomal escape, thereby enhancing the efficiency of siRNA delivery and gene silencing...
  8. ncbi request reprint [Development of multifunctional envelope type artificial viral-like gene delivery system]
    Hideyoshi Harashima
    Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita 12 Nishi 6, Kita ku, Sapporo 060 0812, Japan
    Yakugaku Zasshi 127:1655-72. 2007
    ..This review will provide a future direction of non-viral gene delivery system...
  9. ncbi request reprint Envelope-type lipid nanoparticles incorporating a short PEG-lipid conjugate for improved control of intracellular trafficking and transgene transcription
    Tomoya Masuda
    Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo, Hokkaido 060 0812, Japan
    Biomaterials 30:4806-14. 2009
    ..Collectively, the use of short PEG lipid promises to be useful in developing an efficient non-viral gene vector...
  10. doi request reprint Intracellular fate of octaarginine-modified liposomes in polarized MDCK cells
    Takahiro Fujiwara
    Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo 060 0812, Japan
    Int J Pharm 386:122-30. 2010
    ..Collectively, in polarized MDCK cells, cationic peptide-modified liposomes may be subjected to a different sorting pathway from that used for liposomes composed of cationic lipids...
  11. ncbi request reprint Enhanced gene expression by a novel stearylated INF7 peptide derivative through fusion independent endosomal escape
    Ayman El-Sayed
    Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12 Nishi 6, Kita ku, Sapporo, Hokkaido 060 0812, Japan
    J Control Release 138:160-7. 2009
    ..The intravenous injection of an R8-MEND modified with stearylated INF7 to ICR mice resulted in luciferase expression levels 240-fold higher in liver and 115-fold higher in spleen than that of the R8-MEND...
  12. pmc A DNA microarray-based analysis of the host response to a nonviral gene carrier: a strategy for improving the immune response
    Hiroto Hatakeyama
    Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan
    Mol Ther 19:1487-98. 2011
    ..These findings indicate that a carrier design based on microarray analysis and the manipulation of intracellular trafficking constitutes a rational strategy for reducing the host immune response to NPs...
  13. ncbi request reprint Quantitative comparison of intracellular trafficking and nuclear transcription between adenoviral and lipoplex systems
    Susumu Hama
    Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Hokkaido 060 0812, Japan
    Mol Ther 13:786-94. 2006
    ....
  14. doi request reprint The effect of liposomal size on the targeted delivery of doxorubicin to Integrin αvβ3-expressing tumor endothelial cells
    Golam Kibria
    Laboratory of Innovative Nanomedicine, Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo, Hokkaido 060 0812, Japan
    Biomaterials 34:5617-27. 2013
    ....
  15. doi request reprint Functional improvement of an IRQ-PEG-MEND for delivering genes to the lung
    Taichi Ishitsuka
    Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Sapporo, Hokkaido 060 0812, Japan
    J Control Release 154:77-83. 2011
    ..Overcoming the dilemma of PEGylation is critical issue for in vivo applications of gene delivery targeting endothelial cells...
  16. ncbi request reprint Design of a dual-ligand system using a specific ligand and cell penetrating peptide, resulting in a synergistic effect on selectivity and cellular uptake
    Kazuhiro Takara
    Laboratory of Innovative Nanomedicine, Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo 060 0812, Japan
    Int J Pharm 396:143-8. 2010
    ..The dual-ligand system shows a synergistic effect on cellular uptake. Collectively, the dual-ligand system promises to be useful in the development efficient and specific drug delivery systems...
  17. ncbi request reprint Efficient cytoplasmic protein delivery by means of a multifunctional envelope-type nano device
    Ryosuke Suzuki
    Laboratory for Molecular Design of Pharmaceutics, Faculty of Pharmaceutical Sciences, Hokkaido University, Japan
    Biol Pharm Bull 30:758-62. 2007
    ..These results indicate that the MEND can serve as a useful cytoplasmic delivery system for protein therapy...
  18. ncbi request reprint DNA microarray analysis of type 2 diabetes-related genes co-regulated between white blood cells and livers of diabetic Otsuka Long-Evans Tokushima Fatty (OLETF) rats
    Yasuhiro Hayashi
    Laboratory for Molecular Design of Pharmaceutics, Faculty of Pharmaceutical Sciences, Hokkaido University, Japan
    Biol Pharm Bull 30:763-71. 2007
    ..These results support our hypothesis that the expression levels of type 2 diabetes mellitus related genes in WBC are reflective of those in the liver after the onset of diabetes...
  19. ncbi request reprint Cell cycle dependent transcription, a determinant factor of heterogeneity in cationic lipid-mediated transgene expression
    Hidetaka Akita
    Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Hokkaido 060 0812, Japan
    J Gene Med 9:197-207. 2007
    ..Understandings of intracellular trafficking and the function (transgene expression) of vectors related to cellular physiology are essential in terms of clarifying the mechanism underlying the heterogeneity...
  20. ncbi request reprint Mitochondrial delivery of mastoparan with transferrin liposomes equipped with a pH-sensitive fusogenic peptide for selective cancer therapy
    Yuma Yamada
    Laboratory for Molecular Design of Pharmaceutics, Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Sapporo 060 0812, Japan
    Int J Pharm 303:1-7. 2005
    ..These results demonstrate the utility of MP in Tf-L with GALA for cancer therapy...
  21. doi request reprint An artificial virus-like nano carrier system: enhanced endosomal escape of nanoparticles via synergistic action of pH-sensitive fusogenic peptide derivatives
    Kentaro Sasaki
    Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo, 060 0812, Japan
    Anal Bioanal Chem 391:2717-27. 2008
    ..We concluded that topological control was responsible for the synergistic effect of GALA derivatives introduced on Tf-PEG-L...
  22. doi request reprint Effects of target sequence and sense versus anti-sense strands on gene correction with single-stranded DNA fragments
    Hiroyuki Kamiya
    Faculty of Pharmaceutical Sciences, Hokkaido University, Kita ku, Sapporo, Japan
    J Biochem 144:431-6. 2008
    ..These results suggest that the sense ssDNA fragments are useful for the correction of mutated genes. The variation in the correction efficiency may depend on the sequence of the target position in double-stranded DNA...
  23. doi request reprint An approach to transgene expression in liver endothelial cells using a liposome-based gene vector coated with hyaluronic acid
    Yuma Yamada
    Laboratory for Molecular Design of Pharmaceutics, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo 060 0812, Japan
    J Pharm Sci 102:3119-27. 2013
    ..This finding contributes to our overall knowledge of transgene expression targeted to LECs...
  24. ncbi request reprint DNA microarray analysis of whole blood cells and insulin-sensitive tissues reveals the usefulness of blood RNA profiling as a source of markers for predicting type 2 diabetes
    Yasuhiro Hayashi
    Laboratory of Innovative Nanomedicine, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Hokkaido, Japan
    Biol Pharm Bull 33:1033-42. 2010
    ..These results support our hypothesis that gene expression profiling of whole blood cells might be a useful source of markers to predict the onset of type 2 diabetes...
  25. doi request reprint Ligand-based targeted delivery of a peptide modified nanocarrier to endothelial cells in adipose tissue
    Md Nazir Hossen
    Laboratory of Innovative Nanomedicine, Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo, Hokkaido 060 0812, Japan
    J Control Release 147:261-8. 2010
    ..The selective delivery of tPep-PEG-LPs to endothelial cells in adipose tissue represents a potential approach for the design of diverse nanocarrier-based targeted delivery systems for targeting the vasculature in adipose tissue...
  26. doi request reprint Nanoparticles for ex vivo siRNA delivery to dendritic cells for cancer vaccines: programmed endosomal escape and dissociation
    Hidetaka Akita
    Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo, Hokkaido 060 0812, Japan
    J Control Release 143:311-7. 2010
    ..Collectively, these results show that siRNA loaded in R8/GALA-MEND(SUV) efficiently suppresses endogenous gene expression and consequently enhances dendritic cell-based vaccine potency in vivo...
  27. doi request reprint Systemic delivery of siRNA to tumors using a lipid nanoparticle containing a tumor-specific cleavable PEG-lipid
    Hiroto Hatakeyama
    Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan
    Biomaterials 32:4306-16. 2011
    ..Furthermore, in a DNA microarray analysis in liver and spleen tissue, less gene alternation was found for the PPD-MEND compared to that for the PEG-unmodified MEND due to less accumulation in liver and spleen...
  28. ncbi request reprint High density of octaarginine stimulates macropinocytosis leading to efficient intracellular trafficking for gene expression
    Ikramy A Khalil
    Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo, Hokkaido 060 0812
    J Biol Chem 281:3544-51. 2006
    ..These results show that the density of the R8 peptide on liposomes determines the uptake mechanism and that this is directly linked to intracellular trafficking, resulting in different levels of gene expression...
  29. doi request reprint Efficient MHC class I presentation by controlled intracellular trafficking of antigens in octaarginine-modified liposomes
    Takashi Nakamura
    Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan
    Mol Ther 16:1507-14. 2008
    ..Moreover, R8-Lip showed an efficient antitumor effect in vivo. Therefore, R8-Lip is a promising new carrier for MHC-I-specific antigen presentation...
  30. pmc Therapeutic assessment of cytochrome C for the prevention of obesity through endothelial cell-targeted nanoparticulate system
    Md Nazir Hossen
    Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan
    Mol Ther 21:533-41. 2013
    ..In addition, no detectable hepatotoxicity was found to be associated with this prevention. Thus, the finding highlights the promising potential of CytC for use as an antiobesity drug, when delivered through a nanosystem...
  31. ncbi request reprint Comparison between a multifunctional envelope-type nano device and lipoplex for delivery to the liver
    Jun Yamauchi
    Laboratory of Innovative Nanomedicine, Graduate School of Pharmaceutical Sciences, Hokkaido University, Japan
    Biol Pharm Bull 33:926-9. 2010
    ..Collectively, these data show that a GALA-modified MEND prepared with DC6-14 :DOPE: cholesterol represents a promising device for NPCtargeting gene delivery in vivo...
  32. pmc Particle tracking of intracellular trafficking of octaarginine-modified liposomes: a comparative study with adenovirus
    Hidetaka Akita
    Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo City, Japan
    Mol Ther 18:955-64. 2010
    ..Collectively, the findings reported herein indicate that vesicular transport is a key factor in the cytoplasmic transport of R8-Lips...
  33. ncbi request reprint Efficient short interference RNA delivery to tumor cells using a combination of octaarginine, GALA and tumor-specific, cleavable polyethylene glycol system
    Yu Sakurai
    Laboratory for Molecular Design of Pharmaceutics, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan
    Biol Pharm Bull 32:928-32. 2009
    ..These results demonstrate that the R8/GALA-MEND is a promising delivery system for the transfer of siRNA to tumor cells...
  34. doi request reprint Transgene expression efficiency from plasmid DNA delivered as a complex with histone H3
    Hiroyuki Kamiya
    Faculty of Pharmaceutical Sciences, Hokkaido University, Kita ku, Sapporo, Japan
    Int J Pharm 392:249-53. 2010
    ..Histone H3 suppressed transgene expression in the nucleus in a dose-dependent manner. Our results suggest that the histone-mediated gene delivery is unlikely to be useful, from the viewpoint of the intranuclear disposition...
  35. ncbi request reprint 2-Hydroxy-2'-deoxyadenosine 5'-triphosphate enhances A.T --> C.G mutations caused by 8-hydroxy-2'-deoxyguanosine 5'-triphosphate by suppressing its degradation upon replication in a HeLa extract
    Kazuya Satou
    Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo 060 0812, Japan
    Biochemistry 46:6639-46. 2007
    ..These results highlight the importance of specific hydrolysis of 8-OH-dGTP for the suppression of its induced mutation...
  36. doi request reprint Multi-layered nanoparticles for penetrating the endosome and nuclear membrane via a step-wise membrane fusion process
    Hidetaka Akita
    Hokkaido University, Kita ku, Sapporo, Japan
    Biomaterials 30:2940-9. 2009
    ..Coating the core with the minimum number of nucleus-fusogenic lipid envelopes (i.e., 2) is essential to facilitate transcription. As a result, the T-MEND achieves dramatic levels of transgene expression in non-dividing cells...
  37. ncbi request reprint 2-Methacryloyloxyethyl phosphorylcholine polymer (MPC)-coating improves the transfection activity of GALA-modified lipid nanoparticles by assisting the cellular uptake and intracellular dissociation of plasmid DNA in primary hepatocytes
    Masami Ukawa
    Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo, Hokkaido 060 0812, Japan
    Biomaterials 31:6355-62. 2010
    ..The extensive fusion of the lipid envelope may also reduce electrostatic interactions between mRNA and cationic lipid components, thereby resulting in an enhancement in the translation process...
  38. ncbi request reprint Octaarginine-modified multifunctional envelope-type nano device for siRNA
    Yoshio Nakamura
    Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo 060 0812, Japan
    J Control Release 119:360-7. 2007
    ..Consequently, R8-MEND can deliver condensed siRNA into cells to produce an efficient and persistent silencing effect with minimum cytotoxicity...
  39. ncbi request reprint Mutagenic effects of 8-hydroxy-dGTP in live mammalian cells
    Kazuya Satou
    Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Sapporo 060 0812, Japan
    Free Radic Biol Med 42:1552-60. 2007
    ..coli. These results constitute the first direct evidence to show that 8-OH-dGTP actually induces mutations in living mammalian cells...
  40. ncbi request reprint A multifunctional envelope-type nano device for novel gene delivery of siRNA plasmids
    Rumiko Moriguchi
    Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo 060 0812, Japan
    Int J Pharm 301:277-85. 2005
    ..These results demonstrate that MEND(Prot) is a promising gene delivery system for siRNA expression plasmids with less heterogeneity associated with the transfection...
  41. doi request reprint Vascular-targeted nanotherapy for obesity: unexpected passive targeting mechanism to obese fat for the enhancement of active drug delivery
    Md Nazir Hossen
    Laboratory of Innovative Nanomedicine, Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo, Hokkaido 060 0812, Japan
    J Control Release 163:101-10. 2012
    ..These findings are potentially useful in terms of the design and development of vascular-targeted nanotherapy in the effective control of obesity...
  42. ncbi request reprint Efficient intradermal delivery of superoxide dismutase using a combination of liposomes and iontophoresis for protection against UV-induced skin damage
    Kaoru Kigasawa
    Graduate School of Life Science, Hokkaido University, Sapporo, Hokkaido, Japan
    Biol Pharm Bull 35:781-5. 2012
    ..In conclusion, we succeeded in developing an efficient intradermal SOD delivery system, that would be useful for delivery of other macromolecules...
  43. pmc Dual function MITO-Porter, a nano carrier integrating both efficient cytoplasmic delivery and mitochondrial macromolecule delivery
    Yuma Yamada
    Laboratory for Molecular Design of Pharmaceutics, Faculty of Pharmaceutical Sciences, Hokkaido University, Kita ku, Sapporo, Japan
    Mol Ther 19:1449-56. 2011
    ..We show that the DF-MITO-Porter effectively delivers exogenous macro-biomolecules into the mitochondrial matrix, and provide a demonstration of its potential use in therapies aimed at mitochondrial DNA...
  44. doi request reprint Non-linear pharmacodynamics in the transfection efficiency of a non-viral gene delivery system
    Rumiko Moriguchi
    Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Hokkaido, Japan
    Int J Pharm 363:192-8. 2008
    ..These results emphasize the need to control not only intracellular PK, but also PD for the rational development of non-viral gene delivery systems...
  45. doi request reprint Dual-ligand modification of PEGylated liposomes shows better cell selectivity and efficient gene delivery
    Golam Kibria
    Laboratory of Innovative Nanomedicine, Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo 060 0812, Japan
    J Control Release 153:141-8. 2011
    ....
  46. doi request reprint Dual imaging of mRNA and protein production: an investigation of the mechanism of heterogeneity in cationic lipid-mediated transgene expression
    Hidetaka Akita
    Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Hokkaido 060 0812, Japan
    Int J Pharm 415:218-20. 2011
    ..These findings demonstrate that cell-to-cell differences in the translation process are also a key factor in heterogeneous gene expression...
  47. ncbi request reprint Octaarginine- and octalysine-modified nanoparticles have different modes of endosomal escape
    Ayman El-Sayed
    Faculty of Pharmaceutical Sciences, Hokkaido University, Kita ku, Sapporo, Hokkaido 060 8012, Japan
    J Biol Chem 283:23450-61. 2008
    ..Therefore, it was concluded that R8 on the liposome surface stimulates efficient escape from endocytic vesicles via a fusion mechanism that works at both neutral and acidic pH; in contrast, K8 mediates escape mainly at neutral pH...
  48. doi request reprint Suppression of mutagenesis by 8-hydroxy-2'-deoxyguanosine 5'-triphosphate (7,8-dihydro-8-oxo-2'-deoxyguanosine 5'-triphosphate) by human MTH1, MTH2, and NUDT5
    Mika Hori
    Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo 060 0812, Japan
    Free Radic Biol Med 48:1197-201. 2010
    ..These results indicate that all three of the human MTH1, MTH2, and NUDT5 proteins act as a defense against the mutagenesis induced by oxidized dGTP...
  49. pmc Delivery of macromolecules using arginine-rich cell-penetrating peptides: ways to overcome endosomal entrapment
    Ayman El-Sayed
    Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12 Nishi 6, Kita ku, Sapporo, Hokkaido, 060 0812, Japan
    AAPS J 11:13-22. 2009
    ..The properties of each system and its mechanism of action for the enhancement of endosomal escape are discussed, together with its applications for the delivery of different macromolecules in vitro and, if applicable, in vivo...
  50. ncbi request reprint Intranuclear DNA release is a determinant of transfection activity for a non-viral vector: biocleavable polyrotaxane as a supramolecularly dissociative condenser for efficient intranuclear DNA release
    Yuma Yamada
    Laboratory for Molecular Design of Pharmaceutics, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo 060 0812, Japan
    Biol Pharm Bull 33:1218-22. 2010
    ..Moreover, this system provides a strong support for our hypothesis that intranuclear DNA disposition plays a critical role in gene expression for non-viral vectors...
  51. ncbi request reprint Cationic liposome-mediated gene delivery: biophysical study and mechanism of internalization
    Mohamad Radwan Almofti
    Faculty of Pharmaceutical Sciences, University of Tokushima, Shomachi 1, Tokushima 770 8505, Japan
    Arch Biochem Biophys 410:246-53. 2003
    ..A new perspective for the internalization of large lipoplex particles into cytoplasm is discussed...
  52. ncbi request reprint Evaluation of the nuclear delivery and intra-nuclear transcription of plasmid DNA condensed with micro (mu) and NLS-micro by cytoplasmic and nuclear microinjection: a comparative study with poly-L-lysine
    Hidetaka Akita
    Laboratory for Molecular Design of Pharmaceutics, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Hokkaido, Japan
    J Gene Med 8:198-206. 2006
    ..In an attempt to achieve nuclear delivery, NLS-mu, a novel pDNA condenser, was prepared. This consists of mu, a highly potent polypeptide for condensing the pDNA, and a SV40 T antigen-derived nuclear localization signal (NLS(SV40))...
  53. ncbi request reprint [Development of an efficient short interference RNA (siRNA) delivery system with a new pH-sensitive cationic lipid]
    Yusuke Sato
    Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan
    Yakugaku Zasshi 132:1355-63. 2012
    ..Thus, the YSK05-MEND is a promising siRNA carrier for avoiding the inhibition in intracellular trafficking caused by PEGylation both in vitro and in vivo...
  54. pmc Particle tracking analysis for the intracellular trafficking of nanoparticles modified with African swine fever virus protein p54-derived peptide
    Hidetaka Akita
    Laboratory for Molecular Design of Pharmaceutics, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan
    Mol Ther 21:309-17. 2013
    ..These findings suggest that a portion of the p54(149-161)-modified nanoparticles can use microtubule-dependent transport without the need for an assist by a vesicular sorting system...
  55. doi request reprint A pH-sensitive cationic lipid facilitates the delivery of liposomal siRNA and gene silencing activity in vitro and in vivo
    Yusuke Sato
    Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Hokkaido, Japan
    J Control Release 163:267-76. 2012
    ..Collectively, these data confirm that YSK05 facilitates the endosomal escape of the MEND and thereby enhances the efficacy of siRNA delivery into cytosol and gene silencing...
  56. doi request reprint Size-controlled, dual-ligand modified liposomes that target the tumor vasculature show promise for use in drug-resistant cancer therapy
    Kazuhiro Takara
    Laboratory of Innovative Nanomedicine, Faculty of Pharmaceutical Sciences, Hokkaido University, Hokkaido, Japan
    J Control Release 162:225-32. 2012
    ..Collectively, the dual-ligand LP is promising carrier for the treatment of drug resistant RCC via the disruption of TECs...
  57. doi request reprint Post-nuclear gene delivery events for transgene expression by biocleavable polyrotaxanes
    Yuma Yamada
    Laboratory for Molecular Design of Pharmaceutics, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo 060 0812, Japan
    Biomaterials 33:3952-8. 2012
    ..Such information provides a new viewpoint for the development of cationic polymer-based vectors...
  58. doi request reprint Octaarginine-modified liposomes: enhanced cellular uptake and controlled intracellular trafficking
    Ikramy A Khalil
    Laboratory for Molecular Design of Pharmaceutics, Faculty of Pharmaceutical Sciences, Hokkaido University, Kita ku, Sapporo, Hokkaido, Japan
    Int J Pharm 354:39-48. 2008
    ..In addition, the use of R8-modified liposomes and nano-particles in gene delivery is discussed...
  59. ncbi request reprint Amino acid residues involved in substrate recognition of the Escherichia coli Orf135 protein
    Emiko Iida
    Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo 060 0812, Japan
    Biochemistry 44:5683-9. 2005
    ..coli reduced the level of formation of rpoB mutants elicited by H(2)O(2). These results reveal the residues involved in the substrate binding of the E. coli Orf135 protein...
  60. ncbi request reprint A novel IRQ ligand-modified nano-carrier targeted to a unique pathway of caveolar endocytic pathway
    Diky Mudhakir
    Laboratory for Molecular Design of Pharmaceutics, Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo 060 0812, Japan
    J Control Release 125:164-73. 2008
    ..Moreover, the IRQ has the potential to be useful for delivery therapeutic agents to parenchymal cells via caveolar endocytosis, as this uptake pathway also plays an important role in transcytosis...
  61. ncbi request reprint Recognition of nucleotide analogs containing the 7,8-dihydro-8-oxo structure by the human MTH1 protein
    Hiroyuki Kamiya
    Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo 060 0812
    J Biochem 140:843-9. 2006
    ..These results clarify the effects of the 2-amino group and the two-ring system of the purine base on the recognition by the human MTH1 protein...
  62. doi request reprint Improved gene correction efficiency with a tailed duplex DNA fragment
    Hiroyuki Tsuchiya
    Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo 060 0812, Japan
    Biochemistry 47:8754-9. 2008
    ..This type of gene correction device was also effective for another target gene, the rpsL gene. The results obtained in this study indicate that tailed duplex DNA fragments are effective nucleic acids for gene correction...
  63. doi request reprint KALA-modified multi-layered nanoparticles as gene carriers for MHC class-I mediated antigen presentation for a DNA vaccine
    Sharif M Shaheen
    Laboratory for Molecular Design of Pharmaceutics, Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo 0600812, Japan
    Biomaterials 32:6342-50. 2011
    ..It thus appears that the R8/KALA-T-MEND has the potential for use as a vector in DNA vaccinations...
  64. doi request reprint Mutagenicity of secondary oxidation products of 8-oxo-7,8-dihydro-2'-deoxyguanosine 5'-triphosphate (8-hydroxy-2'- deoxyguanosine 5'-triphosphate)
    Mika Hori
    Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan
    Mutat Res 714:11-6. 2011
    ....
  65. doi request reprint Involvement of specialized DNA polymerases in mutagenesis by 8-hydroxy-dGTP in human cells
    Kazuya Satou
    Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan
    DNA Repair (Amst) 8:637-42. 2009
    ..These results indicate that specialized DNA polymerases are involved in the mutagenesis induced by the oxidized dGTP...
  66. doi request reprint Effects of carriers on transgene expression from plasmids containing a DNA sequence with high histone affinity
    Hiroyuki Kamiya
    Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan
    Int J Pharm 376:99-103. 2009
    ..These results suggest that the left-handedly curved sequence with high histone affinity could increase the transgene expression from a plasmid delivered with carriers...
  67. ncbi request reprint Development of small, homogeneous pDNA particles condensed with mono-cationic detergents and encapsulated in a multifunctional envelope-type nano device
    Ryosuke Suzuki
    Laboratory for Molecular Design of Pharmaceutics, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan
    Biol Pharm Bull 31:1237-43. 2008
    ..Furthermore, the R8-MEND (MCD) were small and homogeneous compared with conventional MEND. These results indicate that R8-MEND (MCD) has potential as a novel non-viral delivery system for clinical application...
  68. ncbi request reprint Factors governing the in vivo tissue uptake of transferrin-coupled polyethylene glycol liposomes in vivo
    Hiroto Hatakeyama
    Laboratory for Molecular Design of Pharmaceutics, Graduate School of Pharmaceutics, Hokkaido University, Sapporo, 060 0812, Japan
    Int J Pharm 281:25-33. 2004
    ..Collectively, these results indicate that size dependency in the uptake of Tf-PEG-L is tissue-dependent and therefore, controlling the size of Tf-PEG-L may be useful for the success of tissue targeting...
  69. ncbi request reprint An assessment of relative transcriptional availability from nonviral vectors
    Rieko Tachibana
    Faculty of Pharmaceutical Sciences, The University of Tokushima, Shomachi 1 78 1, Tokushima 770 8505, Japan
    Int J Pharm 270:315-21. 2004
    ..The experimental design combined with the adoption of pharmacokinetic concepts and principles provide a method to measure TA along with detailed insights into the mechanisms governing gene delivery and expression...
  70. ncbi request reprint MITO-Porter: A liposome-based carrier system for delivery of macromolecules into mitochondria via membrane fusion
    Yuma Yamada
    Laboratory for Molecular Design of Pharmaceutics, Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo 060 0812, Japan
    Biochim Biophys Acta 1778:423-32. 2008
    ..Thus, the MITO-Porter holds promise as an efficacious system for the delivery of both large and small therapeutic molecules into mitochondria...
  71. ncbi request reprint Effects of overexpression and antisense RNA expression of Orf17, a MutT-type enzyme
    Mika Hori
    Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan
    Biol Pharm Bull 29:1087-91. 2006
    ..These results suggest that the role of the Orf17 protein is to back up the MutT function, and to assist in the elimination of 8-hydroxy-2'-deoxyguanosine nucleotides...
  72. doi request reprint Octaarginine- and pH sensitive fusogenic peptide-modified nanoparticles for liver gene delivery
    Ikramy A Khalil
    Laboratory for Molecular Design of Pharmaceutics, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Hokkaido 060 0812, Japan
    J Control Release 156:374-80. 2011
    ..Collectively, these results suggest that the combination of a negatively charged core system and GALA modification of the R8-MEND is useful system for efficiently delivering genes to the liver...
  73. doi request reprint Mitochondrial matrix delivery using MITO-Porter, a liposome-based carrier that specifies fusion with mitochondrial membranes
    Yukari Yasuzaki
    Laboratory for Molecular Design of Pharmaceutics, Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo 060 0812, Japan
    Biochem Biophys Res Commun 397:181-6. 2010
    ..Finally, intracellular observation by confocal laser scanning microscopy clearly verified that the MITO-Porter delivered PI to the mitochondrial matrix...
  74. ncbi request reprint Kinetic analysis of protein production after DNA transfection
    Yuma Yamada
    Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo 060 0812, Japan
    Int J Pharm 299:34-40. 2005
    ..A preliminary pharmacokinetic model is proposed, based on the results obtained...
  75. doi request reprint Effects of base excision repair proteins on mutagenesis by 8-oxo-7,8-dihydroguanine (8-hydroxyguanine) paired with cytosine and adenine
    Tetsuya Suzuki
    Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo 060 0812, Japan
    DNA Repair (Amst) 9:542-50. 2010
    ..These results indicate that all of the above DNA glycosylases suppress mutations caused by 8-oxo-Gua:C in DNA. In contrast, it appears that MUTYH enhances A:T-->C:G mutations caused by 8-oxo-dGTP...
  76. ncbi request reprint Development of lipid particles targeted via sugar-lipid conjugates as novel nuclear gene delivery system
    Tomoya Masuda
    Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12 Nishi 6, Kita ku, Sapporo, Hokkaido 060 0812, Japan
    Biomaterials 29:709-23. 2008
    ..In summary, a novel system for the nuclear delivery of pDNA was successfully developed by using a sugar-modified MEND and by optimizing the lipid envelope stability...
  77. doi request reprint Mitochondrial drug delivery systems for macromolecule and their therapeutic application to mitochondrial diseases
    Yuma Yamada
    Laboratory for Molecular Design of Pharmaceutics, Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo 060 0812, Japan
    Adv Drug Deliv Rev 60:1439-62. 2008
    ..Furthermore, we introduce the novel MITO-Porter--a liposome-based mitochondrial delivery system that functions using a membrane-fusion mechanism...
  78. ncbi request reprint Important amino acids in the phosphohydrolase module of Escherichia coli Orf135
    Hiroyuki Kamiya
    Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo 060 0812, Japan
    Biochem Biophys Res Commun 323:1063-8. 2004
    ..These results indicate the essential residues for the activity of the Orf135 protein...
  79. ncbi request reprint No enhancement of nuclear entry by direct conjugation of a nuclear localization signal peptide to linearized DNA
    Mitsuhide Tanimoto
    Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo 060 0812, Japan
    Bioconjug Chem 14:1197-202. 2003
    ..Thus, we concluded that (i) one or two NLS peptide(s) did not dramatically improve the nuclear entry of DNA and that (ii) chemical modification of DNA reduced the transcription efficiency or stability in the nucleus...
  80. ncbi request reprint Dual hydrolysis of diphosphate and triphosphate derivatives of oxidized deoxyadenosine by Orf17 (NtpA), a MutT-type enzyme
    Mika Hori
    Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo 060 0812, Japan
    DNA Repair (Amst) 4:33-9. 2005
    ..These results suggest that the Orf17 protein may be involved in the hydrolysis of oxidized dATP and dADP...
  81. doi request reprint Nonviral gene delivery
    Hidetaka Akita
    Laboratory for Molecular Design of Pharmaceutics, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan
    Contrib Nephrol 159:13-29. 2008
    ..In particular, comparison of the intracellular trafficking of the engineered devices with that of viruses (i.e. adenovirus) is useful in identifying the rate-limiting intracellular processes of the vectors during development...
  82. ncbi request reprint Multifunctional envelope-type nano device for non-viral gene delivery: concept and application of Programmed Packaging
    Kentaro Kogure
    Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo City, Hokkaido 060 0812, Japan
    J Control Release 122:246-51. 2007
    ..can be the limiting step for Lipofectamin PLUS. These studies indicated the importance of controlling intra-nuclear disposition in the development of an efficient non-viral gene delivery system...
  83. ncbi request reprint Transferrin-modified liposomes equipped with a pH-sensitive fusogenic peptide: an artificial viral-like delivery system
    Tomoyuki Kakudo
    Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo 060 0812, Japan
    Biochemistry 43:5618-28. 2004
    ..With GALA present on the surface, intracellular trafficking of liposomes after receptor-mediated endocytosis could be successfully controlled...
  84. ncbi request reprint Effect of polyethyleneglycol spacer on the binding properties of nuclear localization signal-modified liposomes to isolated nucleus
    Dai Kurihara
    Faculty of Pharmaceutical Sciences, Hokkaido University
    Biol Pharm Bull 32:1303-6. 2009
    ..This information will be useful for the design of nucleus-targeting carriers...
  85. pmc Mutagenic effects of 2-hydroxy-dATP on replication in a HeLa extract: induction of substitution and deletion mutations
    Kazuya Satou
    Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo 060 0812, Japan
    Nucleic Acids Res 31:2570-5. 2003
    ..These results indicate that 2-OH-dATP is mutagenic and that its mutagenicity is suppressed by the MTH1 protein in mammalian cells...
  86. ncbi request reprint Factors affecting SFHR gene correction efficiency with single-stranded DNA fragment
    Hiroyuki Tsuchiya
    Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan
    Biochem Biophys Res Commun 336:1194-200. 2005
    ..The results obtained in this study indicate that length and adenine methylation were important factors affecting the gene correction efficiency, and that the ss DNA fragment was integrated into the double-stranded target DNA...
  87. ncbi request reprint Pharmacokinetic and pharmacodynamic considerations in gene therapy
    Hiroyuki Kamiya
    Laboratory for Molecular, Design of Pharmaceutics, Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo City, Hokkaido 060 0812, Japan
    Drug Discov Today 8:990-6. 2003
    ..The importance of optimizing the system from a transcriptional point of view in the nucleus is also discussed. These new concepts must be integrated to develop an optimized non-viral gene delivery system...
  88. ncbi request reprint Mutagenesis induced by oxidized DNA precursors: roles of Y family DNA polymerases in Escherichia coli
    Kazuya Satou
    Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo 060 0812, Japan
    Chem Res Toxicol 18:1271-8. 2005
    ..These results suggest that the E. coli pol IV was involved in mutagenesis by 2-OH-dATP and that the umuDC gene products play suppressive role(s) in the mutagenesis by damaged nucleotides...
  89. ncbi request reprint Quantitative analysis of correlation between number of nuclear plasmids and gene expression activity after transfection with cationic liposomes
    Rieko Tachibana
    Faculty of Pharmaceutical Sciences, The University of Tokushima, Japan
    Pharm Res 19:377-81. 2002
    ..In this study, quantitative methods were developed to measure plasmids delivered to the nucleus, and the relationship between transfection activity and the number of plasmids in the nucleus were analyzed...
  90. ncbi request reprint Cytotoxic effect of Drosophila deoxynucleoside kinase gene on replicating plasmid in HeLa cells
    Mana Ito
    Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan
    Biol Pharm Bull 33:1223-7. 2010
    ..Our results confirm that transgenes on a replicating plasmid represent an excellent tool for effective protein production and suggest that efficient production of the Dm-dNK protein in tumor cells could be an attractive cancer therapy...
  91. ncbi request reprint Lipoplex size determines lipofection efficiency with or without serum
    Mohamad Radwan Almofti
    Faculty of Pharmaceutical Sciences, The University of Tokushima, Tokushima 770 8505, Japan
    Mol Membr Biol 20:35-43. 2003
    ..These results imply that, by controlling lipoplex size, an efficient lipid delivery system may be achieved for in vitro and in vivo gene therapy...
  92. ncbi request reprint Intranuclear disposition of exogenous DNA in vivo: silencing, methylation and fragmentation
    Hiroshi Ochiai
    Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Sapporo 060 0812, Japan
    FEBS Lett 580:918-22. 2006
    ..Rather, the fragmented DNA was methylated more frequently than the intact plasmid. These results suggest that the CpG methylation and the degradation of exogenous DNA, and its 'silencing', occurred in parallel in the nucleus...
  93. doi request reprint Positive feedback system provides efficient and persistent transgene expression
    Hiroshi Ochiai
    Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo 060 0812, Japan
    Mol Pharm 7:1125-32. 2010
    ..This system would be applicable to gene therapy, in vivo imaging, and biotechnology...
  94. ncbi request reprint Uptake pathways and subsequent intracellular trafficking in nonviral gene delivery
    Ikramy A Khalil
    Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan
    Pharmacol Rev 58:32-45. 2006
    ..In addition, available knowledge concerning cellular entry and the intracellular trafficking of cationic lipid-DNA complexes (lipoplexes) and cationic polymer-DNA complexes (polyplexes) is summarized...
  95. ncbi request reprint Multifunctional envelope-type nano device (MEND) as a non-viral gene delivery system
    Kentaro Kogure
    Faculty of Pharmaceutical Sciences, Hokkaido University, Japan
    Adv Drug Deliv Rev 60:559-71. 2008
    ..Finally, we discuss the rate-limiting step in gene delivery by comparing non-viral and viral gene delivery systems, which clearly indicates the importance of nuclear disposition of pDNA for efficient transfection...
  96. doi request reprint Leptin-derived peptide, a targeting ligand for mouse brain-derived endothelial cells via macropinocytosis
    Mina Tamaru
    Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo, Hokkaido 060 0812, Japan
    Biochem Biophys Res Commun 394:587-92. 2010
    ..This indicates that Lep(70-89)-PEG-LPs are taken up via macropinocytosis and are subject to non-classical intracellular trafficking, resulting in the circumvention of lysosomal degradation in endothelial cells...
  97. ncbi request reprint The location of the left-handedly curved DNA sequence affects exogenous DNA expression in vivo
    Hiroyuki Kamiya
    Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo 060 0812, Japan
    Arch Biochem Biophys 461:7-12. 2007
    ..These results suggest that sequences with high histone affinity could control transgene expression from plasmids in vivo...
  98. ncbi request reprint Mitochondrial drug delivery and mitochondrial disease therapy--an approach to liposome-based delivery targeted to mitochondria
    Yuma Yamada
    Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Sapporo 060 0812, Japan
    Mitochondrion 7:63-71. 2007
    ..Furthermore, novel concept of "Regulation of intramitochondrial trafficking" is proposed herein as a future challenge to the development of a mitochondrial drug delivery system...
  99. doi request reprint NUDT5 hydrolyzes oxidized deoxyribonucleoside diphosphates with broad substrate specificity
    Hiroyuki Kamiya
    Hokkaido University, Sapporo, Japan
    DNA Repair (Amst) 8:1250-4. 2009
    ..These results suggest that the NUDT5 protein eliminates various oxidized deoxyribonucleoside diphosphates from the nucleotide pool and prevents their toxic effects...
  100. ncbi request reprint Construction of a multifunctional envelope-type nano device by a SUV*-fusion method
    Kentaro Sasaki
    Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo 060 0812, Japan
    Int J Pharm 296:142-50. 2005
    ..Consequently, we report on the successful construction of a small and homogenous MEND by a novel SUV*-fusion method...
  101. ncbi request reprint Transient expression of Drosophila melanogaster deoxynucleoside kinase gene enhances cytotoxicity of nucleoside analogs
    Hiroyuki Kamiya
    School of Pharmaceutical Sciences, Hokkaido University, Kita ku, Sapporo, Japan
    Nucleosides Nucleotides Nucleic Acids 25:553-60. 2006
    ..These results suggest that the combination of the transient expression of the Drosophila deoxynucleoside kinase gene and these nucleoside analogs is a candidate for the suicide gene therapy...