Masayuki Shimada

Summary

Affiliation: Hiroshima University
Country: Japan

Publications

  1. pmc LH-induced neuregulin 1 (NRG1) type III transcripts control granulosa cell differentiation and oocyte maturation
    Noritaka Noma
    Laboratory of Reproductive Endocrinology, Graduate School of Biosphere Science, Hiroshima University, Higashi Hiroshima, Hiroshima, Japan
    Mol Endocrinol 25:104-16. 2011
  2. doi request reprint Anti-bacterial factors secreted from cumulus cells of ovulated COCs enhance sperm capacitation during in vitro fertilization
    Masayuki Shimada
    Laboratory of Reproductive Endocrinology, Graduate School of Biosphere Science, Hiroshima University, Hiroshima, Japan
    Am J Reprod Immunol 69:168-79. 2013
  3. pmc Activation of PKA, p38 MAPK and ERK1/2 by gonadotropins in cumulus cells is critical for induction of EGF-like factor and TACE/ADAM17 gene expression during in vitro maturation of porcine COCs
    Yasuhisa Yamashita
    School of Veterinary Medicine, Faculty of Agriculture, Tottori University, 4 101 Koyamachou minami, Tottori, 680 8553, Japan
    J Ovarian Res 2:20. 2009
  4. ncbi request reprint Delay of nuclear maturation and reduction in developmental competence of pig oocytes after mineral oil overlay of in vitro maturation media
    M Shimada
    Graduate School of Biosphere Sciences, Hiroshima University, Higashi Hiroshima, Japan
    Reproduction 124:557-64. 2002
  5. doi request reprint Hyaluronan fragments generated by sperm-secreted hyaluronidase stimulate cytokine/chemokine production via the TLR2 and TLR4 pathway in cumulus cells of ovulated COCs, which may enhance fertilization
    Masayuki Shimada
    Department of Applied Animal Science, Graduate School of Biosphere Science, Hiroshima University, Higashi Hiroshima, Hiroshima, 739 8528, Japan
    Development 135:2001-11. 2008
  6. ncbi request reprint Expression of two progesterone receptor isoforms in cumulus cells and their roles during meiotic resumption of porcine oocytes
    Masayuki Shimada
    Department of Applied Animal Science, Graduate School of Biosphere Science, Hiroshima University, Higashi Hiroshima, Hiroshima 739 8528, Japan
    J Mol Endocrinol 33:209-25. 2004
  7. ncbi request reprint Down-regulated expression of A disintegrin and metalloproteinase with thrombospondin-like repeats-1 by progesterone receptor antagonist is associated with impaired expansion of porcine cumulus-oocyte complexes
    Masayuki Shimada
    Department of Applied Animal Science, Graduate School of Biosphere Science, Hiroshima University, Higashi Hiroshima, Hiroshima 739 8528, Japan
    Endocrinology 145:4603-14. 2004
  8. ncbi request reprint Phosphatidylinositol 3-kinase in cumulus cells is responsible for both suppression of spontaneous maturation and induction of gonadotropin-stimulated maturation of porcine oocytes
    M Shimada
    Laboratory of Animal Reproduction, Graduate School of Biosphere Science, Hiroshima University, Higashi Hiroshima, Hiroshima, 739 8528, Japan
    J Endocrinol 179:25-34. 2003
  9. ncbi request reprint Luteinizing hormone receptor formation in cumulus cells surrounding porcine oocytes and its role during meiotic maturation of porcine oocytes
    Masayuki Shimada
    Laboratories of Animal Reproduction, Graduate School of Biosphere Science, Hiroshima University, Hiroshima, Japan
    Biol Reprod 68:1142-9. 2003
  10. ncbi request reprint Synaptosomal-associated protein 25 gene expression is hormonally regulated during ovulation and is involved in cytokine/chemokine exocytosis from granulosa cells
    Masayuki Shimada
    Department of Applied Animal Science, Graduate School of Biosphere Science, Hiroshima University, 1 4 4, Kagamiyama, Higashi Hiroshima, Hiroshima 739 8528, Japan
    Mol Endocrinol 21:2487-502. 2007

Collaborators

Detail Information

Publications51

  1. pmc LH-induced neuregulin 1 (NRG1) type III transcripts control granulosa cell differentiation and oocyte maturation
    Noritaka Noma
    Laboratory of Reproductive Endocrinology, Graduate School of Biosphere Science, Hiroshima University, Higashi Hiroshima, Hiroshima, Japan
    Mol Endocrinol 25:104-16. 2011
    ..The NRG1 pathway has two roles: one is to enhance AREG-induced progesterone production in granulosa cells, and the other is to regulate oocyte maturation by a cumulus cell-dependent mechanism...
  2. doi request reprint Anti-bacterial factors secreted from cumulus cells of ovulated COCs enhance sperm capacitation during in vitro fertilization
    Masayuki Shimada
    Laboratory of Reproductive Endocrinology, Graduate School of Biosphere Science, Hiroshima University, Hiroshima, Japan
    Am J Reprod Immunol 69:168-79. 2013
    ..The aim of this study was to find immune-related genes expressed in cumulus cells of ovulated cumulus oocyte complexes (COCs) and to clear the functional roles during fertilization process...
  3. pmc Activation of PKA, p38 MAPK and ERK1/2 by gonadotropins in cumulus cells is critical for induction of EGF-like factor and TACE/ADAM17 gene expression during in vitro maturation of porcine COCs
    Yasuhisa Yamashita
    School of Veterinary Medicine, Faculty of Agriculture, Tottori University, 4 101 Koyamachou minami, Tottori, 680 8553, Japan
    J Ovarian Res 2:20. 2009
    ..In the present study, we investigated which signaling pathways are involved in the gene expression of EGF-like factor and in Tace/Adam17 expression in cumulus cells of porcine COC during in vitro maturation...
  4. ncbi request reprint Delay of nuclear maturation and reduction in developmental competence of pig oocytes after mineral oil overlay of in vitro maturation media
    M Shimada
    Graduate School of Biosphere Sciences, Hiroshima University, Higashi Hiroshima, Japan
    Reproduction 124:557-64. 2002
    ..These adverse effects of oil may be explained by absorption by the oil of cumulus-secreted steroids or by the release of toxic compounds into the medium...
  5. doi request reprint Hyaluronan fragments generated by sperm-secreted hyaluronidase stimulate cytokine/chemokine production via the TLR2 and TLR4 pathway in cumulus cells of ovulated COCs, which may enhance fertilization
    Masayuki Shimada
    Department of Applied Animal Science, Graduate School of Biosphere Science, Hiroshima University, Higashi Hiroshima, Hiroshima, 739 8528, Japan
    Development 135:2001-11. 2008
    ....
  6. ncbi request reprint Expression of two progesterone receptor isoforms in cumulus cells and their roles during meiotic resumption of porcine oocytes
    Masayuki Shimada
    Department of Applied Animal Science, Graduate School of Biosphere Science, Hiroshima University, Higashi Hiroshima, Hiroshima 739 8528, Japan
    J Mol Endocrinol 33:209-25. 2004
    ....
  7. ncbi request reprint Down-regulated expression of A disintegrin and metalloproteinase with thrombospondin-like repeats-1 by progesterone receptor antagonist is associated with impaired expansion of porcine cumulus-oocyte complexes
    Masayuki Shimada
    Department of Applied Animal Science, Graduate School of Biosphere Science, Hiroshima University, Higashi Hiroshima, Hiroshima 739 8528, Japan
    Endocrinology 145:4603-14. 2004
    ..These results suggest that the expression and induction of ADAMTS-1 through receptor-mediated action of progesterone in cumulus cells is one of the essential requirements for gonadotropin-regulated cumulus expansion of porcine COCs...
  8. ncbi request reprint Phosphatidylinositol 3-kinase in cumulus cells is responsible for both suppression of spontaneous maturation and induction of gonadotropin-stimulated maturation of porcine oocytes
    M Shimada
    Laboratory of Animal Reproduction, Graduate School of Biosphere Science, Hiroshima University, Higashi Hiroshima, Hiroshima, 739 8528, Japan
    J Endocrinol 179:25-34. 2003
    ..Judging from these results, it is estimated that PI 3-kinase in cumulus cells is required for both the suppression of spontaneous meiotic resumption and the induction of gonadotropin-stimulated meiotic resumption...
  9. ncbi request reprint Luteinizing hormone receptor formation in cumulus cells surrounding porcine oocytes and its role during meiotic maturation of porcine oocytes
    Masayuki Shimada
    Laboratories of Animal Reproduction, Graduate School of Biosphere Science, Hiroshima University, Hiroshima, Japan
    Biol Reprod 68:1142-9. 2003
    ..Treatment of oocytes with FSH and 0.5 mM IBMX to express LH receptor in cumulus cells while holding oocytes at the GV II stage is a very beneficial way to produce in vitro-matured oocytes, which have high developmental competence...
  10. ncbi request reprint Synaptosomal-associated protein 25 gene expression is hormonally regulated during ovulation and is involved in cytokine/chemokine exocytosis from granulosa cells
    Masayuki Shimada
    Department of Applied Animal Science, Graduate School of Biosphere Science, Hiroshima University, 1 4 4, Kagamiyama, Higashi Hiroshima, Hiroshima 739 8528, Japan
    Mol Endocrinol 21:2487-502. 2007
    ..From these results, we conclude that progesterone-progesterone receptor-mediated SNAP25 expression in cumulus oocyte complexes and granulosa cells regulates cytokine and chemokine secretion via an exocytosis system...
  11. doi request reprint Sequential exposure of porcine cumulus cells to FSH and/or LH is critical for appropriate expression of steroidogenic and ovulation-related genes that impact oocyte maturation in vivo and in vitro
    Ikkou Kawashima
    Department of Applied Animal Science, Graduate School of Biosphere Science, Hiroshima University, 1 4 4, Kagamiyama, Higashi Hiroshima, Hiroshima 739 8528, Japan
    Reproduction 136:9-21. 2008
    ....
  12. pmc MAPK3/1 (ERK1/2) in ovarian granulosa cells are essential for female fertility
    Heng Yu Fan
    Department of Molecular and Cellular Biology, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX 77030, USA
    Science 324:938-41. 2009
    ..Thus, ERK1/2 and C/EBPbeta constitute an in vivo LH-regulated signaling pathway that controls ovulation- and luteinization-related events...
  13. ncbi request reprint Progesterone is essential for maintenance of Tace/Adam17 mRNA expression, but not EGF-like factor, in cumulus cells, which enhances the EGF receptor signaling pathway during in vitro maturation of porcine COCs
    Yasuhisa Yamashita
    School of Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori, Japan
    J Reprod Dev 56:315-23. 2010
    ....
  14. ncbi request reprint Hormone-induced expression of tumor necrosis factor alpha-converting enzyme/A disintegrin and metalloprotease-17 impacts porcine cumulus cell oocyte complex expansion and meiotic maturation via ligand activation of the epidermal growth factor receptor
    Yasuhisa Yamashita
    Department of Applied Animal Science, Graduate School of Biosphere Science, Hiroshima University, 1 4 4 Kagamiyama, Higashi Hiroshima, Japan
    Endocrinology 148:6164-75. 2007
    ..Thus, TACE/ADAM17 is induced and the activity is involved in porcine COC expansion as well as oocyte meiotic maturation through the activation of EGF receptor in cumulus cells...
  15. pmc Beta-catenin (CTNNB1) promotes preovulatory follicular development but represses LH-mediated ovulation and luteinization
    Heng Yu Fan
    Department of Molecular and Cellular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA
    Mol Endocrinol 24:1529-42. 2010
    ....
  16. ncbi request reprint LH reduces proliferative activity of cumulus cells and accelerates GVBD of porcine oocytes
    Tetsuji Okazaki
    Laboratory of Animal Reproduction, Graduate School of Biosphere Science, Hiroshima University, Higashi Hiroshima, Hiroshima 739 8528, Japan
    Mol Cell Endocrinol 209:43-50. 2003
    ....
  17. ncbi request reprint Gonadotropin-induced delta14-reductase and delta7-reductase gene expression in cumulus cells during meiotic resumption of porcine oocytes
    Yasuhisa Yamashita
    Department of Applied Animal Science, Graduate School of Biosphere Science, Hiroshima University, Higashi Hiroshima, Hiroshima 739 8528, Japan
    Endocrinology 146:186-94. 2005
    ..These results imply that gonadotropin-induced expression and function of Delta14-reductase and Delta7-reductase in cumulus cells contribute to oocyte meiotic resumption via a progesterone-dependent pathway...
  18. ncbi request reprint Roles of cAMP in regulation of both MAP kinase and p34(cdc2) kinase activity during meiotic progression, especially beyond the MI stage
    Masayuki Shimada
    Faculty of Applied Biological Science, Hiroshima University, Higashi Hiroshima, Hiroshima 739 8528, Japan
    Mol Reprod Dev 62:124-31. 2002
    ....
  19. ncbi request reprint Induced expression of pattern recognition receptors in cumulus oocyte complexes: novel evidence for innate immune-like functions during ovulation
    Masayuki Shimada
    Department of Molecular Cellular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA
    Mol Endocrinol 20:3228-39. 2006
    ..Collectively, these results provide novel evidence that cumulus cells as well as granulosa cells express innate immune related genes that may play critical roles in surveillance and cell survival during the ovulation process...
  20. ncbi request reprint Timing of MAP kinase inactivation effects on emission of polar body in porcine oocytes activated by Ca2+ ionophore
    Junya Ito
    Department of Applied Animal Science, Graduate School of Biosphere Sciences, Hiroshima University, Higashi Hiroshima, Japan
    Mol Reprod Dev 70:64-9. 2005
    ..This method can be available for successfully ICSI in low response species of oocyte activation to Ca(2+) ionophore including pig...
  21. pmc Endogenous acetaldehyde toxicity during antral follicular development in the mouse ovary
    Tomoko Kawai
    Graduate School of Biosphere Science, Hiroshima University, Higashi Hiroshima, Hiroshima 739 8528, Japan
    Reprod Toxicol 33:322-30. 2012
    ..These results indicate that acetaldehyde is generated as a by-product during steroidogenesis and can exert toxic effects to impair the differentiation of granulosa cells, reduce ovulation and decrease oocyte quality...
  22. ncbi request reprint Paracrine and autocrine regulation of epidermal growth factor-like factors in cumulus oocyte complexes and granulosa cells: key roles for prostaglandin synthase 2 and progesterone receptor
    Masayuki Shimada
    Department of Molecular Cellular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA
    Mol Endocrinol 20:1352-65. 2006
    ....
  23. ncbi request reprint The role of calcium/calmodulin-dependent protein kinase II on the inactivation of MAP kinase and p34cdc2 kinase during fertilization and activation in pig oocytes
    Junya Ito
    Laboratory of Animal Reproduction, Graduate School of Biosphere Sciences, Hiroshima University, Higashi Hiroshima, Japan
    Reproduction 128:409-15. 2004
    ..In conclusion, the activation of CaMKII by artificial stimuli or sperm stimulated the disruption of cyclin B1 and the inactivation of p34cdc2 kinase, but did not affect MAP kinase inactivation during oocyte activation in pigs...
  24. doi request reprint Possible involvement of phosphatidylinositol 3-kinase in the maintenance of metaphase II arrest in porcine oocytes matured in vitro
    Junya Ito
    Laboratory of Animal Reproduction, School of Veterinary Medicine, Azabu University, Sagamihara, Japan
    Anim Sci J 81:42-7. 2010
    ..Therefore, we concluded that PI3K is involved in the maintenance of MAPK activity in matured porcine oocytes...
  25. ncbi request reprint FSH and LH induce progesterone production and progesterone receptor synthesis in cumulus cells: a requirement for meiotic resumption in porcine oocytes
    Masayuki Shimada
    Faculty of Applied Biological Science, Hiroshima University, Higashi Hiroshima, Hiroshima, 739 8528, Japan
    Mol Hum Reprod 8:612-8. 2002
    ....
  26. ncbi request reprint Production of progesterone from de novo-synthesized cholesterol in cumulus cells and its physiological role during meiotic resumption of porcine oocytes
    Yasuhisa Yamashita
    Laboratory of Animal Reproduction, Graduate School of Biosphere Science, Hiroshima University, Higashi Hiroshima, Hiroshima, 739 8528, Japan
    Biol Reprod 68:1193-8. 2003
    ..These results demonstrate that a high level of progesterone produced by cumulus cells was responsible for an acceleration of GVBD in porcine oocytes...
  27. pmc Selective expression of KrasG12D in granulosa cells of the mouse ovary causes defects in follicle development and ovulation
    Heng Yu Fan
    Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Development 135:2127-37. 2008
    ..Transient but not sustained activation of RAS in granulosa cells is therefore crucial for directing normal follicle development and initiating the ovulation process...
  28. ncbi request reprint TAF4b, a TBP associated factor, is required for oocyte development and function
    Allison E Falender
    Department of Molecular and Cellular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Dev Biol 288:405-19. 2005
    ..Thus, infertility in Taf4b null female mice is associated with defects in early follicle formation, oocyte maturation, and zygotic cleavage following ovulation and fertilization...
  29. ncbi request reprint Mitogen-activated protein kinase kinase inhibitor suppresses cyclin B1 synthesis and reactivation of p34cdc2 kinase, which improves pronuclear formation rate in matured porcine oocytes activated by Ca2+ ionophore
    Junya Ito
    Laboratory of Animal Reproduction, Graduate School of Biosphere Sciences, Hiroshima University, Higashi Hiroshima, 739 8528, Japan
    Biol Reprod 70:797-804. 2004
    ....
  30. ncbi request reprint Phosphatidylinositol 3-kinase in cumulus cells and oocytes is responsible for activation of oocyte mitogen-activated protein kinase during meiotic progression beyond the meiosis I stage in pigs
    M Shimada
    Faculty of Applied Biological Science, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8528, Japan
    Biol Reprod 64:1106-14. 2001
    ..Moreover, gap junctional communications between cumulus cells and oocytes may be closed by phosphorylation of connexin-43 through PI 3-kinase activation in cumulus cells, leading to the activation of MAP kinase in porcine oocytes...
  31. ncbi request reprint Dynamic changes of connexin-43, gap junctional protein, in outer layers of cumulus cells are regulated by PKC and PI 3-kinase during meiotic resumption in porcine oocytes
    M Shimada
    Faculty of Applied Biological Science, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8528, Japan
    Biol Reprod 64:1255-63. 2001
    ..Moreover, the connexin-43 in the inner layers of cumulus cells is phosphorylated during meiotic progression beyond the MI stage, regardless of MAP kinase activation in cumulus cells surrounding the oocyte...
  32. ncbi request reprint Artificial insemination with seminal plasma improves the reproductive performance of frozen-thawed boar epididymal spermatozoa
    Tetsuji Okazaki
    Laboratory of Animal Reproduction, Graduate School of Biosphere Science, Hiroshima University, Higashi Hiroshima, Hiroshima, 739 8528, Japan
    J Androl 33:990-8. 2012
    ..From these results, we concluded that the addition of seminal plasma to the thawing solution is a key step in obtaining an optimal number of piglets by artificial insemination using frozen-thawed boar epididymal spermatozoa...
  33. pmc EGF-like factors induce expansion of the cumulus cell-oocyte complexes by activating calpain-mediated cell movement
    Ikko Kawashima
    Graduate School of Biosphere Science, Hiroshima University, Higashi Hiroshima, Hiroshima, 739 8528, Japan
    Endocrinology 153:3949-59. 2012
    ..The latter are controlled by calpain activation downstream of the EGF receptor activation of the Ca(2+) pathway and ERK1/2 pathways...
  34. pmc Interleukin-6: an autocrine regulator of the mouse cumulus cell-oocyte complex expansion process
    Zhilin Liu
    Department of Molecular and Cellular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA
    Endocrinology 150:3360-8. 2009
    ..Collectively, these data indicate that IL-6 alone can act as a potent autocrine regulator of ovarian cumulus cell function, COC expansion, and oocyte competence...
  35. ncbi request reprint Effect of protein kinase C activator on mitogen-activated protein kinase and p34(cdc2) kinase activity during parthenogenetic activation of porcine oocytes by calcium ionophore
    Junya Ito
    Laboratory of Animal Reproduction, Graduate School of Biosphere Sciences, Hiroshima University, Higashi Hiroshima, 739 8528, Japan
    Biol Reprod 69:1675-82. 2003
    ..In conclusion, the parthenogenetic activation of porcine oocytes was mediated by the inactivation of p34(cdc2) kinase via a calcium-dependent pathway and thereafter by the inactivation of MAP kinase via a PKC-dependent pathway...
  36. ncbi request reprint Inhibition of phosphatidylinositol 3-kinase or mitogen-activated protein kinase kinase leads to suppression of p34(cdc2) kinase activity and meiotic progression beyond the meiosis I stage in porcine oocytes surrounded with cumulus cells
    M Shimada
    Faculty of Applied Biological Science, Hiroshima University, Higashi-Hiroshima, Hiroshima, 739-8528, Japan
    Biol Reprod 65:442-8. 2001
    ....
  37. pmc Toll-like receptors (TLR) 2 and 4 on human sperm recognize bacterial endotoxins and mediate apoptosis
    Youko Fujita
    Laboratory of Reproductive Endocrinology, Department of Applied Animal Science, Graduate School of Biosphere Science, Hiroshima University, Higashi Hiroshima, Hiroshima 739 8528, Japan
    Hum Reprod 26:2799-806. 2011
    ....
  38. doi request reprint Immune-like mechanisms in ovulation
    JoAnne S Richards
    Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, USA
    Trends Endocrinol Metab 19:191-6. 2008
    ....
  39. ncbi request reprint Mice null for Frizzled4 (Fzd4-/-) are infertile and exhibit impaired corpora lutea formation and function
    Minnie Hsieh
    Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    Biol Reprod 73:1135-46. 2005
    ..Thus, Fzd4 appears to impact the formation of the corpus luteum by mechanisms that more closely phenocopy Prlr null mice...
  40. ncbi request reprint Gene expression profiles of cumulus cell oocyte complexes during ovulation reveal cumulus cells express neuronal and immune-related genes: does this expand their role in the ovulation process?
    Inmaculada Hernandez-Gonzalez
    Department of Molecular and Cellular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA
    Mol Endocrinol 20:1300-21. 2006
    ....
  41. doi request reprint Polymyxin B neutralizes bacteria-released endotoxin and improves the quality of boar sperm during liquid storage and cryopreservation
    Tetsuji Okazaki
    Department of Applied Animal Science, Graduate School of Biosphere Science, Hiroshima University, Hiroshima 739 8528, Japan
    Theriogenology 74:1691-700. 2010
    ....
  42. pmc The involvement of the Toll-like receptor family in ovulation
    Zhilin Liu
    Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, USA
    J Assist Reprod Genet 25:223-8. 2008
    ..However, the expression and function of innate immune cell-related genes in non-immune cells within the ovary has been reported recently and provides a novel and important regulatory system during ovulation...
  43. ncbi request reprint Enzyme immunoassay of progesterone in the feces from beef cattle to monitor the ovarian cycle
    N Isobe
    Graduate School for International Development and Cooperation, Hiroshima University, Higashi Hiroshima 739 8529, Japan
    Anim Reprod Sci 87:1-10. 2005
    ..These results suggest that the present EIA is suitable to measure the progesterone in cattle feces and can monitor ovarian cycle...
  44. pmc Adiponectin and its receptors modulate granulosa cell and cumulus cell functions, fertility, and early embryo development in the mouse and human
    JoAnne S Richards
    Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas, USA
    Fertil Steril 98:471-9.e1. 2012
    ..To study the expression and function of adiponectin and its receptors in mouse and human follicle cells and in early embryo development...
  45. doi request reprint New strategies of boar sperm cryopreservation: development of novel freezing and thawing methods with a focus on the roles of seminal plasma
    Tetsuji Okazaki
    Smaller Livestock and Environment Section, Livestock Research Institute, Oita Prefectural Agriculture, Forestry and Fisheries Research Center, Oita
    Anim Sci J 83:623-9. 2012
    ..On the other hand, seminal plasma suppresses cryo-capacitation induced by thawing stress. On the basis of these findings, we designed a novel protocol of AI using frozen-thawed boar sperm...
  46. ncbi request reprint Fecal progestagen and estrone during pregnancy in a giraffe: a case report
    Naoki Isobe
    Graduate School of Biosphere Science, Hiroshima University, Japan
    J Reprod Dev 53:159-64. 2007
    ..The hormone dynamics of the third parity suggest the possibility of early pregnancy diagnosis by measuring progestagen or estrone between days 30 and 120 after mating...
  47. ncbi request reprint Survival of boar spermatozoa frozen in diluents of varying osmolality
    W X Zeng
    Animal Reproduction, Faculty of Applied Biological Science, Hiroshima University, Higashi-Hiroshima, Japan
    Theriogenology 56:447-58. 2001
    ..These observations obtained in the present study indicate that moderately hypertonic BF5 diluents are favorable for the cryopreservation of boar spermatozoa...
  48. ncbi request reprint Motility and penetration competence of frozen-thawed miniature pig spermatozoa are substantially altered by exposure to seminal plasma before freezing
    N Kawano
    Animal Reproduction Lab, Graduate School of Biosphere Science, Hiroshima University, Higashi Hiroshima, Hiroshima 739 8528, Japan
    Theriogenology 61:351-64. 2004
    ..We concluded that ejaculated seminal plasma contained some factor(s) that modified the sperm before freezing and reduced the freezability and post-thaw penetration competence of spermatozoa...
  49. ncbi request reprint Contribution of high p34cdc2 kinase activity to premature chromosome condensation of injected somatic cell nuclei in rat oocytes
    Junya Ito
    National Institute for Physiological Sciences, Okazaki 444 8787, Japan
    Reproduction 129:171-80. 2005
    ....
  50. ncbi request reprint Assessment of human oocyte developmental competence by cumulus cell morphology and circulating hormone profile
    Chikako Sato
    St Luke Clinic, 5 Tsumori Tomioka, Oita 870 047, Japan
    Reprod Biomed Online 14:49-56. 2007
    ..0. It is concluded that an optimal window of hormonal profile(s) may exist for follicle aspiration to obtain grade B COC in FSH-stimulated human IVM cycles...
  51. ncbi request reprint Involvement of Ca2+-dependent proteasome in the degradation of both cyclin B1 and Mos during spontaneous activation of matured rat oocytes
    Junya Ito
    Section of Molecular Genetics, Center for Brain Experiment, National Institute for Physiological Sciences, Okazaki, Aichi 444 8787, Japan
    Theriogenology 67:475-85. 2007
    ..In contrast, Wistar oocytes had a relatively low cytostatic factor activity; rapid decrease of Mos/MEK/MAPK failed to stabilize both cyclin B1 and Mos, and these oocytes were likely to spontaneously activate...