Kiyoshi Miyagawa

Summary

Affiliation: Hiroshima University
Country: Japan

Publications

  1. pmc A role for RAD54B in homologous recombination in human cells
    Kiyoshi Miyagawa
    Department of Molecular Pathology, Research Institute for Radiation Biology and Medicine, Hiroshima University, 1 2 3 Kasumi, Minami Ku, Hiroshima 734 8553, Japan
    EMBO J 21:175-80. 2002
  2. ncbi Clinical relevance of the homologous recombination machinery in cancer therapy
    Kiyoshi Miyagawa
    Department of Radiation Biology, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Cancer Sci 99:187-94. 2008
  3. ncbi Haploinsufficiency of RAD51B causes centrosome fragmentation and aneuploidy in human cells
    Osamu Date
    Department of Human Genetics, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima, Japan
    Cancer Res 66:6018-24. 2006
  4. pmc Haploinsufficiency of the Mus81-Eme1 endonuclease activates the intra-S-phase and G2/M checkpoints and promotes rereplication in human cells
    Takashi Hiyama
    Department of Human Genetics, Hiroshima University 1 2 3 Kasumi, Minami Ku, Hiroshima 734 8553, Japan
    Nucleic Acids Res 34:880-92. 2006
  5. pmc XRCC3 deficiency results in a defect in recombination and increased endoreduplication in human cells
    Takashi Yoshihara
    Department of Human Genetics, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima, Japan
    EMBO J 23:670-80. 2004
  6. pmc Human Rad54B is a double-stranded DNA-dependent ATPase and has biochemical properties different from its structural homolog in yeast, Tid1/Rdh54
    Kozo Tanaka
    Department of Molecular Pathology, Research Institute for Radiation Biology and Medicine, Hiroshima University, 1 2 3 Kasumi, Minami Ku, Hiroshima 734 8553, Japan
    Nucleic Acids Res 30:1346-53. 2002
  7. ncbi Ocular development-associated gene (ODAG), a novel gene highly expressed in ocular development
    Takanori Tsuruga
    Department of Ophthalmology, Hiroshima University School of Medicine, 1 2 3 Kasumi, Minami Ku, Hiroshima 734 8551, Japan
    Gene 290:125-30. 2002
  8. ncbi Gene trap mutagenesis-based forward genetic approach reveals that the tumor suppressor OVCA1 is a component of the biosynthetic pathway of diphthamide on elongation factor 2
    Yoshitaka Nobukuni
    Department of Human Genetics, Research Institute for Radiation Biology and Medicine, Hiroshima University, 1 2 3 Kasumi, Minami Ku, Hiroshima 734 8553, Japan
    J Biol Chem 280:10572-7. 2005
  9. ncbi [Hyperlipidemia: complex pathophysiology caused by multiple genetic and environmental factors--in considering the approaches to preventive medicine]
    Yoshitaka Nobukuni
    Department of Human Genetics, Research Institute for Radiation Biology and Medicine, Hiroshima University, 1 2 3 Kasumi, Minami Ku, Hiroshima 734 8553, Japan
    Nihon Eiseigaku Zasshi 60:426-41. 2005
  10. pmc Stimulation of Dmc1-mediated DNA strand exchange by the human Rad54B protein
    Naoyuki Sarai
    RIKEN Genomic Sciences Center, 1 7 22 Suehiro cho, Tsurumi, Yokohama 230 0045, Japan
    Nucleic Acids Res 34:4429-37. 2006

Collaborators

Detail Information

Publications17

  1. pmc A role for RAD54B in homologous recombination in human cells
    Kiyoshi Miyagawa
    Department of Molecular Pathology, Research Institute for Radiation Biology and Medicine, Hiroshima University, 1 2 3 Kasumi, Minami Ku, Hiroshima 734 8553, Japan
    EMBO J 21:175-80. 2002
    ..Our findings provide the first genetic evidence that the mitotic recombination pathway is functionally conserved from yeast to humans...
  2. ncbi Clinical relevance of the homologous recombination machinery in cancer therapy
    Kiyoshi Miyagawa
    Department of Radiation Biology, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Cancer Sci 99:187-94. 2008
    ..These findings highlight the importance of HR machinery in cancer therapy...
  3. ncbi Haploinsufficiency of RAD51B causes centrosome fragmentation and aneuploidy in human cells
    Osamu Date
    Department of Human Genetics, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima, Japan
    Cancer Res 66:6018-24. 2006
    ..These findings suggest that the proper biallelic expression of RAD51B is required for the maintenance of chromosome integrity in human cells...
  4. pmc Haploinsufficiency of the Mus81-Eme1 endonuclease activates the intra-S-phase and G2/M checkpoints and promotes rereplication in human cells
    Takashi Hiyama
    Department of Human Genetics, Hiroshima University 1 2 3 Kasumi, Minami Ku, Hiroshima 734 8553, Japan
    Nucleic Acids Res 34:880-92. 2006
    ..These findings suggest that a complex network of the checkpoint pathways that respond to DNA double-strand breaks may participate in some of the phenotypes associated with Mus81 or Eme1 deficiency...
  5. pmc XRCC3 deficiency results in a defect in recombination and increased endoreduplication in human cells
    Takashi Yoshihara
    Department of Human Genetics, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima, Japan
    EMBO J 23:670-80. 2004
    ..These observations offer the first genetic evidence for the association between homologous recombination and replication initiation having a role in cancer susceptibility...
  6. pmc Human Rad54B is a double-stranded DNA-dependent ATPase and has biochemical properties different from its structural homolog in yeast, Tid1/Rdh54
    Kozo Tanaka
    Department of Molecular Pathology, Research Institute for Radiation Biology and Medicine, Hiroshima University, 1 2 3 Kasumi, Minami Ku, Hiroshima 734 8553, Japan
    Nucleic Acids Res 30:1346-53. 2002
    ..In contrast, neither human Rad51 nor Dmc1 directly interacts with hRad54B. Although hRad54B is the putative counterpart of Tid1/Rdh54, our findings suggest that hRad54B behaves differently from Tid1/Rdh54...
  7. ncbi Ocular development-associated gene (ODAG), a novel gene highly expressed in ocular development
    Takanori Tsuruga
    Department of Ophthalmology, Hiroshima University School of Medicine, 1 2 3 Kasumi, Minami Ku, Hiroshima 734 8551, Japan
    Gene 290:125-30. 2002
    ..Mouse ODAG-specific mRNA expression was detected in various mouse tissues within the eye at P2 and P7, whereas it was not detected anywhere at P14, suggesting that ODAG may play a role in eye development...
  8. ncbi Gene trap mutagenesis-based forward genetic approach reveals that the tumor suppressor OVCA1 is a component of the biosynthetic pathway of diphthamide on elongation factor 2
    Yoshitaka Nobukuni
    Department of Human Genetics, Research Institute for Radiation Biology and Medicine, Hiroshima University, 1 2 3 Kasumi, Minami Ku, Hiroshima 734 8553, Japan
    J Biol Chem 280:10572-7. 2005
    ..A functional genetic approach utilizing the random gene trap mutants library of mammalian cells should become a useful strategy to identify the genes responsible for specific phenotypes...
  9. ncbi [Hyperlipidemia: complex pathophysiology caused by multiple genetic and environmental factors--in considering the approaches to preventive medicine]
    Yoshitaka Nobukuni
    Department of Human Genetics, Research Institute for Radiation Biology and Medicine, Hiroshima University, 1 2 3 Kasumi, Minami Ku, Hiroshima 734 8553, Japan
    Nihon Eiseigaku Zasshi 60:426-41. 2005
    ..Elucidation of the genetic and environmental factors and detailed understanding of the molecular pathophysiology of hyperlipidemia are essential in the pursuit of evidence-based preventive medicine and health policy...
  10. pmc Stimulation of Dmc1-mediated DNA strand exchange by the human Rad54B protein
    Naoyuki Sarai
    RIKEN Genomic Sciences Center, 1 7 22 Suehiro cho, Tsurumi, Yokohama 230 0045, Japan
    Nucleic Acids Res 34:4429-37. 2006
    ..Therefore, Rad54B may stimulate the Dmc1-mediated DNA strand exchange by stabilizing the nucleoprotein filament, which is formed on the ssDNA tails produced at DSB sites during homologous recombination...
  11. pmc Biochemical analysis of the N-terminal domain of human RAD54B
    Naoyuki Sarai
    Systems and Structural Biology Center, Yokohama Institute, RIKEN, 1 7 22 Suehiro cho, Tsurumi, Yokohama 230 0045, Japan
    Nucleic Acids Res 36:5441-50. 2008
    ..Thus, RAD54B binding may affect the quaternary structure of DMC1. These observations suggest that the N-terminal domain of RAD54B plays multiple roles of in homologous recombination...
  12. ncbi Structural basis for octameric ring formation and DNA interaction of the human homologous-pairing protein Dmc1
    Takashi Kinebuchi
    Protein Research Group, RIKEN Genomic Sciences Center, 1 7 22 Suehiro cho, Tsurumi, Yokohama 230 0045, Japan
    Mol Cell 14:363-74. 2004
    ..The inner patch binds both single-stranded and double-stranded DNAs, while the outer one binds single-stranded DNA. Based on these results, we propose a model for the interaction of the Dmc1 rings with DNA...
  13. ncbi High contribution contrast between the genes of eosinophil peroxidase and IL-4 receptor alpha-chain in Japanese cedar pollinosis
    Hiroyuki Nakamura
    Department of Environmental Medicine, Kochi Medical School, Nankoku, Japan
    J Allergy Clin Immunol 112:1127-31. 2003
    ..Japanese cedar pollinosis is the most common form of hayfever in Japan in spring and has remarkably increased since 1960...
  14. ncbi Genotypes and haplotypes of CCR2 and CCR3 genes in Japanese cedar pollinosis
    Hiroyuki Nakamura
    Department of Environmental Medicine, Kochi University School of Medicine, Kochi, Nankoku, Japan
    Int Arch Allergy Immunol 142:329-34. 2007
    ..The frequency of haplotype 64Ile/780C/51C in pollinosis was significantly higher than in controls. Our results suggest that CCR2 and CCR3 genes are candidate genes for Japanese cedar pollinosis...
  15. pmc Bloom's syndrome helicase and Mus81 are required to induce transient double-strand DNA breaks in response to DNA replication stress
    Tsutomu Shimura
    Laboratory of Molecular Pharmacology, Center for Cancer Research, NCI, NIH, Bethesda, MD 20892 4255, USA
    J Mol Biol 375:1152-64. 2008
    ....
  16. ncbi Current topics in DNA double-strand break repair
    Junya Kobayashi
    Department of Genome Repair Dynamics, Radiation Biology Center, Kyoto University, Kyoto, Japan
    J Radiat Res 49:93-103. 2008
    ..In addition to the function of these proteins in DSB repair, the biological function of nuclear foci formation following DSB induction is discussed...
  17. ncbi The human pre-B cell line Nalm-6 is highly proficient in gene targeting by homologous recombination
    Noritaka Adachi
    Kihara Institute for Biological Research, Graduate School of Integrated Science, Yokohama City University, Japan
    DNA Cell Biol 25:19-24. 2006
    ..Expression analysis revealed a lack of MSH2 expression in this cell line. As Nalm-6 has a stable neardiploid karyotype with normal p53 status, our results underscore the usefulness of Nalm-6 for gene knockout studies in humans...