Affiliation: Hiroshima University
- Kinoshita E, Kinoshita Kikuta E, Koike T. The Cutting Edge of Affinity Electrophoresis Technology. Proteomes. 2015;3:42-55 pubmed publisher..Here, we describe advances in analytical techniques involving affinity electrophoresis that have appeared during the last five years. ..
- Kinoshita E, Kinoshita Kikuta E, Koike T. Zn(II)-Phos-Tag SDS-PAGE for Separation and Detection of a DNA Damage-Related Signaling Large Phosphoprotein. Methods Mol Biol. 2017;1599:113-126 pubmed publisher..The procedure described in this protocol requires a completion time of approximately 5 h from the beginning of gel preparation to the end of electrophoresis. ..
- Kinoshita E, Kinoshita Kikuta E, Matsubara M, Yamada S, Nakamura H, Shiro Y, et al. Separation of phosphoprotein isotypes having the same number of phosphate groups using phosphate-affinity SDS-PAGE. Proteomics. 2008;8:2994-3003 pubmed publisher..As for FixJ, on the other hand, two monophosphoisotypes were detected as two distinct migration bands. One is a well-known isotype phosphorylated at the Asp-54. The other is a novel isotype phosphorylated at the His-84. ..
- Shiba A, Kinoshita Kikuta E, Kinoshita E, Koike T. TAMRA/TAMRA Fluorescence Quenching Systems for the Activity Assay of Alkaline Phosphatase. Sensors (Basel). 2017;17: pubmed publisher..By examining the initial slope of this time-dependent fluorescence recovery, we succeeded in evaluating the 50% inhibitory concentrations of orthovanadate toward two AP isozymes under near-physiological conditions. ..