Teruo Shimmen

..Successful biochemical and biophysical approaches to studying myosins have extensively promoted the understanding of the molecular mechanism underlying these phenomena...

..The narrower the chloroplast-removed area, the less significant the extent of OH(-) extrusion. This is the first success in inducing a new alkaline band at a target position in Characeae...

..Experiments under K(+)-induced depolarization unequivocally showed that the Cl(-) channel at the nodal end of the receptor cell was activated upon cutting the victim cell...

..I suggest that the change in the trans-nodal hydraulic pressure difference mechanically stretches the plasma membrane, and this induces the electrical depolarization...

..Cells could not form alkaline bands, when plasmolysis was induced by application of sorbitol at a higher concentration. Addition of 10 mM CaCl(2) could ameliorate the inhibition caused by plasmolyis...

..It is suggested that MYA2 targets the peroxisome through an interaction with AtRabC2a...

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..Furthermore, this myosin was co-fractionated with GFP-ER by sucrose density gradient centrifugation. From these findings, it was suggested that the 175 kDa myosin is a molecular motor responsible for translocating ER in BY-2 cells...

..In combination with osmotic shock, 10 mM K+ could induce a long-lasting depolarization of the node. These electrical characteristics of the node were suggested to be responsible for the electrical response to wounding in Characeae...

..The results of water permeability measurements suggest the presence of an AQP (aquaporin) in the membrane of the CV (contractile vacuole) in Amoeba proteus [Nishihara, Shimmen and Sonobe (2004) Cell Struct. Funct. 29, 85-90]...

..We hypothesize that, in the presence of MAP200, a catastrophe-prone microtubule lattice forms occasionally when elongated but nonadjacent protofilaments make lateral contacts...

..However, a possibility of direct inhibition of the proton pump by bromoxynil flowed into the cell could not be excluded. In the present study, the direct effect of bromoxynil on the proton pump was unequivocally excluded...

..These results suggest that NtTHO2 is a multifunctional protein that participates in mRNA metabolism, and also functions within the cortical microtubules and mitotic spindle...

..When the extracellular KCl concentration was increased, alkaline band formation was extensively inhibited. Electrophysiological analysis unequivocally showed the need for inner negative membrane potential for alkaline band formation...

..It was suggested that the mesh-like AF structure is indispensable for the elongation of rhizoids. A possible organization mechanism of the mesh-like AF structure was discussed...

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..When GDP was added to the NtDRP3 fraction, the filaments disappeared and many particles appeared. Biochemical analysis revealed that NtDRP3 could bind to and bundle both microtubules and actin filaments in vitro...

..The decrease in the electro-chemical potential gradient for H(+) across the plasma membrane appears to result in inhibition of cell growth and disturbance of intracellular homeostasis in the presence of BX...

..Acidification of the cytosol by bromoxynil was confirmed by experiments using pollen tubes. Based on the acidity of the apoplast, the herbicide action of bromoxynil in higher plants was discussed...

..The single-cell system developed in the present study seems suitable for analysis of gene expression during rhizoid differentiation of Spirogyra...

..A callosic substance was also synthesized during formation of the conjugation tube...

..On the hydrophilic substratum, however, only Bandeiraea (Griffonia) simplicifolia lectin strongly stained the rhizoids...

..Either treatment with Gd(3+) or decrease in extracellular Ca(2+) resulted in inhibition of all these phenomena, suggesting possible involvement of stretch-activated ion channel in position sensing by terminal cells...

..These results supported the hypothesis that K+ released from the killed cell is responsible for generation of the wound-induced membrane depolarization...

..We propose that the peroxisome is one of the cargos translocated by MYA2 on actin filaments...

..In cylindrical leaflet cells in Chara corallina, transverse MTs were observed. These results imply that C. inflata is a morphological mutant lacking a mechanism for orienting cortical MTs transverse in leaflet cells...

..This result suggested that the factor is slowly turning over even in the absence of osmotic stimulation...

..From these results, we concluded that the 21-kDa polypeptide is a low-molecular-weight CyP. The present study showed that CyP in the pollen of higher plants is released into the extracellular matrix under unfavorable conditions...

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..Based on our biochemical and immunofluorescence findings, the function of MAP200 in MT polymerization is discussed...

..It was suggested that the contraction of isolated CV was caused by increase in the tension of its membrane by ATP...

..The membrane resistance increased significantly. It was found that the tonoplasts also maintained their semipermeability during glutaraldehyde treatment...

..These results indicated that plant villins are widely distributed and involved in the organization of actin filaments into bundles throughout the plant kingdom...

..The role of the actin-myosin system in various plant cell functions is becoming evident. The present article reviews progress in studies on cytoplasmic streaming over the past 50 years...

..islandicum's proliferation. "Long cells" reported previously were found to comprise several cylindrical cells that align in tandem...

..However, it inhibited cMT reorientation induced by gravitropic bending and by forced bending. Involvement of the stretch-activated channel in mechano-sensitive orientation of cMT was suggested...

..The stronger the mechanical stimulus, the larger was the increase in the cytoplasmic level of Ca2+. It is proposed that the first step of receptor potential is an activation of mechanosensitive Ca2+ channels at the plasma membrane...

..These results indicate that myosin XI is highly specialized for generation of fast processive movement with concomitantly low forces...

..Our results have broad implications for understanding the interactions between mechano-perception and salinity stress in plants...

..We conclude that defective F-actin polymerization caused the aberrant cell morphology in a dominant-negative manner, and that ACT2 functions in cell elongation and root hair formation...