Toshiyuki Matsunaga

Summary

Affiliation: Gifu Pharmaceutical University
Country: Japan

Publications

  1. doi request reprint Aldo-keto reductase 1B10 promotes development of cisplatin resistance in gastrointestinal cancer cells through down-regulating peroxisome proliferator-activated receptor-γ-dependent mechanism
    Toshiyuki Matsunaga
    Laboratory of Biochemistry, Gifu Pharmaceutical University, Gifu 501 1196, Japan Electronic address
    Chem Biol Interact 256:142-53. 2016
  2. doi request reprint Exposure to 9,10-phenanthrenequinone accelerates malignant progression of lung cancer cells through up-regulation of aldo-keto reductase 1B10
    Toshiyuki Matsunaga
    Laboratory of Biochemistry, Gifu Pharmaceutical University, Gifu 501 1196, Japan Electronic address
    Toxicol Appl Pharmacol 278:180-9. 2014
  3. pmc Aldo-Keto Reductase 1B10 and Its Role in Proliferation Capacity of Drug-Resistant Cancers
    Toshiyuki Matsunaga
    Laboratory of Biochemistry, Gifu Pharmaceutical University Gifu, Japan
    Front Pharmacol 3:5. 2012
  4. ncbi request reprint Reduction of cytotoxic p-quinone metabolites of tert-butylhydroquinone by human aldo-keto reductase (AKR) 1B10
    Toshiyuki Matsunaga
    Gifu Pharmaceutical University, Gifu, Japan
    Drug Metab Pharmacokinet 27:553-8. 2012
  5. doi request reprint Pathophysiological roles of aldo-keto reductases (AKR1C1 and AKR1C3) in development of cisplatin resistance in human colon cancers
    Toshiyuki Matsunaga
    Laboratory of Biochemistry, Gifu Pharmaceutical University, Gifu, Japan
    Chem Biol Interact 202:234-42. 2013
  6. ncbi request reprint 9,10-phenanthrenequinone induces monocytic differentiation of U937 cells through regulating expression of aldo-keto reductase 1C3
    Toshiyuki Matsunaga
    Laboratory of Biochemistry, Gifu Pharmaceutical University, Gifu, Japan
    Biol Pharm Bull 35:1598-602. 2012
  7. doi request reprint 9,10-Phenanthrenequinone promotes secretion of pulmonary aldo-keto reductases with surfactant
    Toshiyuki Matsunaga
    Laboratory of Biochemistry, Gifu Pharmaceutical University, Gifu 501 1196, Japan
    Cell Tissue Res 347:407-17. 2012
  8. doi request reprint Toxicity against gastric cancer cells by combined treatment with 5-fluorouracil and mitomycin c: implication in oxidative stress
    Toshiyuki Matsunaga
    Laboratory of Biochemistry, Gifu Pharmaceutical University, Gifu, 502 8585, Japan
    Cancer Chemother Pharmacol 66:517-26. 2010
  9. doi request reprint Nitric oxide mitigates apoptosis in human endothelial cells induced by 9,10-phenanthrenequinone: role of proteasomal function
    Toshiyuki Matsunaga
    Laboratory of Biochemistry, Gifu Pharmaceutical University, 5 6 1 Mitahora Higashi, Gifu 502 8585, Japan
    Toxicology 268:191-7. 2010
  10. doi request reprint Potent and selective inhibition of the tumor marker AKR1B10 by bisdemethoxycurcumin: probing the active site of the enzyme with molecular modeling and site-directed mutagenesis
    Toshiyuki Matsunaga
    Laboratory of Biochemistry, Gifu Pharmaceutical University, Gifu 502 8585, Japan
    Biochem Biophys Res Commun 389:128-32. 2009

Collaborators

Detail Information

Publications79

  1. doi request reprint Aldo-keto reductase 1B10 promotes development of cisplatin resistance in gastrointestinal cancer cells through down-regulating peroxisome proliferator-activated receptor-γ-dependent mechanism
    Toshiyuki Matsunaga
    Laboratory of Biochemistry, Gifu Pharmaceutical University, Gifu 501 1196, Japan Electronic address
    Chem Biol Interact 256:142-53. 2016
    ..These results suggest that combined treatment with the AKR1B10 inhibitor and PPARγ ligand is an effective adjuvant therapy for overcoming CDDP resistance of gastrointestinal cancer cells. ..
  2. doi request reprint Exposure to 9,10-phenanthrenequinone accelerates malignant progression of lung cancer cells through up-regulation of aldo-keto reductase 1B10
    Toshiyuki Matsunaga
    Laboratory of Biochemistry, Gifu Pharmaceutical University, Gifu 501 1196, Japan Electronic address
    Toxicol Appl Pharmacol 278:180-9. 2014
    ..Collectively, these results suggest that AKR1B10 is a key regulator involved in overgrowth and malignant progression of the lung cancer cells through ROS production due to 9,10-PQ redox-cycling...
  3. pmc Aldo-Keto Reductase 1B10 and Its Role in Proliferation Capacity of Drug-Resistant Cancers
    Toshiyuki Matsunaga
    Laboratory of Biochemistry, Gifu Pharmaceutical University Gifu, Japan
    Front Pharmacol 3:5. 2012
    ..Recent developments of AKR1B10 inhibitors and their usefulness in restoring sensitivity to anticancer drugs are also reviewed...
  4. ncbi request reprint Reduction of cytotoxic p-quinone metabolites of tert-butylhydroquinone by human aldo-keto reductase (AKR) 1B10
    Toshiyuki Matsunaga
    Gifu Pharmaceutical University, Gifu, Japan
    Drug Metab Pharmacokinet 27:553-8. 2012
    ..These results suggest a role for the enzyme in protection at least against the toxicity of the two p-quinone metabolites of BHQ...
  5. doi request reprint Pathophysiological roles of aldo-keto reductases (AKR1C1 and AKR1C3) in development of cisplatin resistance in human colon cancers
    Toshiyuki Matsunaga
    Laboratory of Biochemistry, Gifu Pharmaceutical University, Gifu, Japan
    Chem Biol Interact 202:234-42. 2013
    ..Collectively, these results suggest the involvement of up-regulated AKR1C1, AKR1C3 and proteasome in CDDP resistance of colon cancers and support a chemotherapeutic role for their inhibitors...
  6. ncbi request reprint 9,10-phenanthrenequinone induces monocytic differentiation of U937 cells through regulating expression of aldo-keto reductase 1C3
    Toshiyuki Matsunaga
    Laboratory of Biochemistry, Gifu Pharmaceutical University, Gifu, Japan
    Biol Pharm Bull 35:1598-602. 2012
    ..These results suggest that ROS formation during 9,10-PQ treatment acutely leads to apoptosis of U937 cells and the initiation of monocytic differentiation, which proceeds after the provisional overexpression of AKR1C3...
  7. doi request reprint 9,10-Phenanthrenequinone promotes secretion of pulmonary aldo-keto reductases with surfactant
    Toshiyuki Matsunaga
    Laboratory of Biochemistry, Gifu Pharmaceutical University, Gifu 501 1196, Japan
    Cell Tissue Res 347:407-17. 2012
    ..Thus, the AKR1C enzymes secreted in pulmonary surfactants probably participate in the toxic mechanism triggered by 9,10-PQ...
  8. doi request reprint Toxicity against gastric cancer cells by combined treatment with 5-fluorouracil and mitomycin c: implication in oxidative stress
    Toshiyuki Matsunaga
    Laboratory of Biochemistry, Gifu Pharmaceutical University, Gifu, 502 8585, Japan
    Cancer Chemother Pharmacol 66:517-26. 2010
    ..We examined the administration sequence of combining 5FU with MMC to maximize toxicity against a human gastric cancer cell line, and then investigated the possible molecular mechanisms underlying the observed toxic effects...
  9. doi request reprint Nitric oxide mitigates apoptosis in human endothelial cells induced by 9,10-phenanthrenequinone: role of proteasomal function
    Toshiyuki Matsunaga
    Laboratory of Biochemistry, Gifu Pharmaceutical University, 5 6 1 Mitahora Higashi, Gifu 502 8585, Japan
    Toxicology 268:191-7. 2010
    ....
  10. doi request reprint Potent and selective inhibition of the tumor marker AKR1B10 by bisdemethoxycurcumin: probing the active site of the enzyme with molecular modeling and site-directed mutagenesis
    Toshiyuki Matsunaga
    Laboratory of Biochemistry, Gifu Pharmaceutical University, Gifu 502 8585, Japan
    Biochem Biophys Res Commun 389:128-32. 2009
    ....
  11. doi request reprint Involvement of an aldo-keto reductase (AKR1C3) in redox cycling of 9,10-phenanthrenequinone leading to apoptosis in human endothelial cells
    Toshiyuki Matsunaga
    Laboratory of Biochemistry, Gifu Pharmaceutical University, 5 6 1 Mitahora Higashi, Gifu, Japan
    Chem Biol Interact 181:52-60. 2009
    ..These results suggest that AKR1C3 is a key enzyme in the initial step of 9,10-PQ-induced cytotoxicity in HAECs...
  12. doi request reprint L-Xylulose reductase is involved in 9,10-phenanthrenequinone-induced apoptosis in human T lymphoma cells
    Toshiyuki Matsunaga
    Laboratory of Biochemistry, Gifu Pharmaceutical University, 5 6 1 Mitahora Higashi, Gifu 502 8585, Japan
    Free Radic Biol Med 44:1191-202. 2008
    ..Moreover, the 9,10-PQ-induced apoptosis was partially inhibited by the pretreatment with XR-specific inhibitors. These results suggest that initially produced ROS induce XR, which accelerates the generation of ROS...
  13. doi request reprint Protective effect of rat aldo-keto reductase (AKR1C15) on endothelial cell damage elicited by 4-hydroxy-2-nonenal
    Toshiyuki Matsunaga
    Laboratory of Biochemistry, Gifu Pharmaceutical University, Gifu, Japan
    Chem Biol Interact 191:364-70. 2011
    ..Collectively, these data indicate an anti-atherogenic function of AKR1C15 through the protection of endothelial cells from damage elicited by toxic lipids such as HNE...
  14. doi request reprint Involvement of the aldo-keto reductase, AKR1B10, in mitomycin-c resistance through reactive oxygen species-dependent mechanisms
    Toshiyuki Matsunaga
    Laboratory of Biochemistry, Gifu Pharmaceutical University, Gifu, Japan
    Anticancer Drugs 22:402-8. 2011
    ..These results suggest a role of AKR1B10 in the development of MMC resistance, which may be mediated by its ability to detoxify cytotoxic aldehydes including 4-hydroxy-2-nonenal...
  15. doi request reprint Aldo-keto reductase 1C15 as a quinone reductase in rat endothelial cell: its involvement in redox cycling of 9,10-phenanthrenequinone
    Toshiyuki Matsunaga
    Laboratory of Biochemistry, Gifu Pharmaceutical University, Gifu 501 1196, Japan
    Free Radic Res 45:848-57. 2011
    ..9,10-PQ also provoked the AKR1C15 up-regulation, which sensitized against the quinone toxicity. These results suggest the presence of a negative feedback loop exacerbating the quinone toxicity in rat endothelial cells...
  16. doi request reprint Characterization of human DHRS4: an inducible short-chain dehydrogenase/reductase enzyme with 3beta-hydroxysteroid dehydrogenase activity
    Toshiyuki Matsunaga
    Laboratory of Biochemistry, Gifu Pharmaceutical University, 5 6 1 Mitahora Higashi, Gifu 502 8585, Japan
    Arch Biochem Biophys 477:339-47. 2008
    ..The results suggest a novel mechanism of cold inactivation and role of the inducible human DHRS4 in 3beta-hydroxysteroid synthesis and xenobiotic carbonyl metabolism...
  17. doi request reprint Rabbit 3-hydroxyhexobarbital dehydrogenase is a NADPH-preferring reductase with broad substrate specificity for ketosteroids, prostaglandin D₂, and other endogenous and xenobiotic carbonyl compounds
    Satoshi Endo
    Gifu Pharmaceutical University, Gifu 502 1196, Japan Electronic address
    Biochem Pharmacol 86:1366-75. 2013
    ....
  18. doi request reprint Modulation of activity and inhibitor sensitivity of rabbit aldose reductase-like protein (AKR1B19) by oxidized glutathione and SH-reagents
    Satoshi Endo
    Gifu Pharmaceutical University, Gifu 501 1196, Japan
    Chem Biol Interact 202:146-52. 2013
    ..Furthermore, such an activity alteration by GSSG was not detected in AKR1B10 and rat ARLPs, suggesting the presence of a GSSG-binding site near Cys299 in AKR1B19...
  19. doi request reprint Characterization of a rat NADPH-dependent aldo-keto reductase (AKR1B13) induced by oxidative stress
    Satoshi Endo
    Laboratory of Biochemistry, Gifu Pharmaceutical University, Gifu, Japan
    Chem Biol Interact 178:151-7. 2009
    ..These results indicate that AKR1B13 differs from AKR1B8 in tissue distribution and gene regulation, and suggest that it functions as a defense system against oxidative stress in rat tissues...
  20. doi request reprint Properties and tissue distribution of a novel aldo-keto reductase encoding in a rat gene (Akr1b10)
    Satoshi Endo
    Gifu Pharmaceutical University, Japan
    Arch Biochem Biophys 503:230-7. 2010
    ..The results suggest that R1B10 functions as a defense system against oxidative stress and glycation in rat tissues...
  21. doi request reprint Chromene-3-carboxamide derivatives discovered from virtual screening as potent inhibitors of the tumour maker, AKR1B10
    Satoshi Endo
    Laboratory of Biochemistry, Gifu Pharmaceutical University, Gifu 501 1196, Japan
    Bioorg Med Chem 18:2485-90. 2010
    ..7nM, and the metabolism of farnesal and 4-hydroxynonenal in the AKR1B10-overexpressed cells from 0.1microM with an IC(50) value equal to 0.8microM...
  22. pmc Roles of rat and human aldo-keto reductases in metabolism of farnesol and geranylgeraniol
    Satoshi Endo
    Gifu Pharmaceutical University, Gifu, Japan
    Chem Biol Interact 191:261-8. 2011
    ..Thus, AKRs (1C15 in rats, and 1B10 and 1C3 in humans) may play an important role in controlling the bioavailability of FOH and GGOH...
  23. ncbi request reprint Substrate specificity and inhibitor sensitivity of rabbit 20α-hydroxysteroid dehydrogenase
    Satoshi Endo
    Laboratory of Biochemistry, Gifu Pharmaceutical University
    Biol Pharm Bull 36:1514-8. 2013
    ..AKR1C5 was potently and competitively inhibited by flavonoids such as kaempferol and quercetin, suggesting that its activity is affected by ingested flavonoids...
  24. doi request reprint Kinetic studies of AKR1B10, human aldose reductase-like protein: endogenous substrates and inhibition by steroids
    Satoshi Endo
    Laboratory of Biochemistry, Gifu Pharmaceutical University, 5 6 1 Mitahora Higashi, Gifu 502 8585, Japan
    Arch Biochem Biophys 487:1-9. 2009
    ....
  25. doi request reprint Synthesis and structure-activity relationship of 2-phenyliminochromene derivatives as inhibitors for aldo-keto reductase (AKR) 1B10
    Satoshi Endo
    Laboratory of Biochemistry, Gifu Pharmaceutical University, Gifu 501 1196, Japan Electronic address
    Bioorg Med Chem 21:6378-84. 2013
    ....
  26. doi request reprint Characterization of rabbit aldose reductase-like protein with 3β-hydroxysteroid dehydrogenase activity
    Satoshi Endo
    Laboratory of Biochemistry, Gifu Pharmaceutical University, Gifu 501 1196, Japan
    Arch Biochem Biophys 527:23-30. 2012
    ..Single and double mutations (F303Q, M304S and F303Q/M304S) significantly impaired this activity, suggesting the two residues play critical roles in recognition of the steroidal substrate...
  27. doi request reprint Activation of aldo-keto reductase family member 1B14 (AKR1B14) by bile acids: Activation mechanism and bile acid-binding site
    Satoshi Endo
    Laboratory of Biochemistry, Gifu Pharmaceutical University, 1 25 4 Daigaku nishi, Gifu 501 1196, Japan
    Biochimie 93:1476-86. 2011
    ....
  28. ncbi request reprint Characterization of rat and mouse NAD+-dependent 3alpha/17beta/20alpha-hydroxysteroid dehydrogenases and identification of substrate specificity determinants by site-directed mutagenesis
    Satoshi Endo
    Laboratory of Biochemistry, Gifu Pharmaceutical University, Mitahora Higashi, Gifu 502 8585, Japan
    Arch Biochem Biophys 467:76-86. 2007
    ..The replacement of Asp128, Phe129 and Ser137 of AKR1C16 with the corresponding residues (Glu, Ser and Phe, respectively) of AKR1C24 increased the catalytic efficiency for 17beta- and 20alpha-hydroxysteroids...
  29. doi request reprint Molecular determinants for the stereospecific reduction of 3-ketosteroids and reactivity towards all-trans-retinal of a short-chain dehydrogenase/reductase (DHRS4)
    Satoshi Endo
    Laboratory of Biochemistry, Gifu Pharmaceutical University, 5 6 1 Mitahora Higashi, Gifu 502 8585, Japan
    Arch Biochem Biophys 481:183-90. 2009
    ....
  30. ncbi request reprint Molecular cloning of a novel type of rat cytoplasmic 17beta-hydroxysteroid dehydrogenase distinct from the type 5 isozyme
    Shuhei Ishikura
    Laboratory of Biochemistry, Gifu Pharmaceutical University, Gifu 502 8585
    J Biochem 139:1053-63. 2006
    ..In addition, TBER2 was identified as 3alpha-hydroxysteroid dehydrogenase on chromatographic analysis of the enzyme activities in rat liver cytosol and characterization of the recombinant 3alpha-hydroxysteroid dehydrogenase...
  31. ncbi request reprint Rat aldose reductase-like protein (AKR1B14) efficiently reduces the lipid peroxidation product 4-oxo-2-nonenal
    Satoshi Endo
    Laboratory of Biochemistry, Gifu Pharmaceutical University, Daigaku Nishi, Gifu 501 1196, Japan
    Biol Pharm Bull 33:1886-90. 2010
    ..Kinetic analyses of the oxidoreduction and dead-end inhibition suggest that the reaction follows an ordered sequential mechanism...
  32. doi request reprint Human carbonyl reductase 4 is a mitochondrial NADPH-dependent quinone reductase
    Satoshi Endo
    Laboratory of Biochemistry, Gifu Pharmaceutical University, 5 6 1 Mitahora Higashi, Gifu 502 8585, Japan
    Biochem Biophys Res Commun 377:1326-30. 2008
    ..We further demonstrate that the in vitro quinone reduction by CBR4 generates superoxide through the redox cycling, and suggest that the enzyme may be involved in the induction of apoptosis by cytotoxic 9,10-phenanthrenequinone...
  33. doi request reprint Selective inhibition of the tumor marker aldo-keto reductase family member 1B10 by oleanolic acid
    Mayuko Takemura
    Laboratory of Biochemistry, Gifu Pharmaceutical University, Gifu 501 1196, Japan
    J Nat Prod 74:1201-6. 2011
    ..Thus, the selective and potent inhibition of AKR1B10 by 1 may be related to a possible cancer inhibitory role...
  34. doi request reprint Characterization of rabbit morphine 6-dehydrogenase and two NAD(+)-dependent 3α(17β)-hydroxysteroid dehydrogenases
    Satoshi Endo
    Gifu Pharmaceutical University, Gifu 501 1196, Japan
    Arch Biochem Biophys 529:131-9. 2013
    ..The site-directed mutagenesis of Tyr118 and Phe310 in AKR1C27 to the corresponding residues (Phe and Ile, respectively) in AKR1C28 produced an enzyme that was similar to AKR1C28, suggesting their key roles in ligand binding...
  35. doi request reprint Design, synthesis and evaluation of caffeic acid phenethyl ester-based inhibitors targeting a selectivity pocket in the active site of human aldo-keto reductase 1B10
    Midori Soda
    Gifu Pharmaceutical University, 1 25 4 Daigaku nishi, Gifu 501 1196, Japan
    Eur J Med Chem 48:321-9. 2012
    ..Additionally, the sub-μM concentration of 10c significantly suppressed the farnesal metabolism and cellular proliferation in AKR1B10-overexpressing cells...
  36. ncbi request reprint Rat NAD+-dependent 3alpha-hydroxysteroid dehydrogenase (AKR1C17): a member of the aldo-keto reductase family highly expressed in kidney cytosol
    Masaharu Sanai
    Laboratory of Biochemistry, Gifu Pharmaceutical University, Mitahora Higashi, Gifu 502 8585, Japan
    Arch Biochem Biophys 464:122-9. 2007
    ..In addition, the replacement of Gln270 and Glu276 of AKR1C17 with the corresponding residues of NADP(H)-dependent 3alpha-HSD resulted in a switch in favor of NADP(+) specificity, suggesting their key roles in coenzyme specificity...
  37. ncbi request reprint Substrate specificity of a mouse aldo-keto reductase (AKR1C12)
    Satoshi Endo
    Laboratory of Biochemistry, Gifu Pharmaceutical University, Japan, and Hospital for Sick Children, Toronto, Ontario, Canada
    Biol Pharm Bull 29:2488-92. 2006
    ..The results, together with the intracellular high ratio of NAD+/NADH, suggest that AKR1C12 functions as a dehydrogenase for the endogenous hydroxysteroids and geranylgeraniol in mouse stomach and myeloid cells...
  38. ncbi request reprint Selective inhibition of the tumor marker AKR1B10 by antiinflammatory N-phenylanthranilic acids and glycyrrhetic acid
    Satoshi Endo
    Laboratory of Biochemistry, Gifu Pharmaceutical University, Daigaku Nishi, Gifu 501 1196, Japan
    Biol Pharm Bull 33:886-90. 2010
    ..Thus, the potent and selective inhibition may be related to the cancer chemopreventive roles of the drugs, and their structural features may facilitate the design of new anti-cancer agents targeting AKR1B10...
  39. ncbi request reprint Characterization of an oligomeric carbonyl reductase of dog liver: its identity with peroxisomal tetrameric carbonyl reductase
    Satoshi Endo
    Laboratory of Biochemistry, Gifu Pharmaceutical University, Mitahora Higashi, Gifu 502 8585, Japan
    Biol Pharm Bull 30:1787-91. 2007
    ..Thus, dog oligomeric CR is PTCR, and may play a role in retinoid metabolism as a retinal reductase...
  40. doi request reprint Up-Regulation of Carbonyl Reductase 1 Renders Development of Doxorubicin Resistance in Human Gastrointestinal Cancers
    Toshiyuki Matsunaga
    Laboratory of Biochemistry, Gifu Pharmaceutical University
    Biol Pharm Bull 38:1309-19. 2015
    ..Thus, CBR1 may promote development of DOX resistance through detoxification of cytotoxic aldehydes, rather than the drug's metabolism. ..
  41. doi request reprint Acquisition of doxorubicin resistance facilitates migrating and invasive potentials of gastric cancer MKN45 cells through up-regulating aldo-keto reductase 1B10
    Yoshifumi Morikawa
    Laboratory of Biochemistry, Gifu Pharmaceutical University, Gifu 501 1196, Japan
    Chem Biol Interact 230:30-9. 2015
    ..These results suggest that AKR1B10 is a DOX-resistance gene in the gastric cancer cells, and is responsible for elevating the migrating and invasive potentials of the cells through induction of MMP2. ..
  42. ncbi request reprint Inhibition of human aldose reductase-like protein (AKR1B10) by α- and γ-mangostins, major components of pericarps of mangosteen
    Midori Soda
    Laboratory of Biochemistry, Gifu Pharmaceutical University, Gifu 501 1196, Japan
    Biol Pharm Bull 35:2075-80. 2012
    ....
  43. ncbi request reprint Enzymatic characteristics of an aldo-keto reductase family protein (AKR1C15) and its localization in rat tissues
    Satoshi Endo
    Laboratory of Biochemistry, Gifu Pharmaceutical University, Mitahora Higashi, Gifu 502 8585, Japan
    Arch Biochem Biophys 465:136-47. 2007
    ..These results suggest that AKR1C15 plays a role in retinoid, steroid, isoprenoid and carbohydrate metabolism, as well as a defense system, protecting against reactive carbonyl compounds...
  44. doi request reprint Clathrin-dependent endocytosis of claudin-2 by DFYSP peptide causes lysosomal damage in lung adenocarcinoma A549 cells
    Akira Ikari
    The Laboratory of Biochemistry, Department of Biopharmaceutical Sciences, Gifu Pharmaceutical University, Japan Electronic address
    Biochim Biophys Acta 1848:2326-36. 2015
    ..Our data indicate that DFYSP peptide increases the accumulation of the peptide and claudin-2 into the lysosome, resulting in lysosomal damage. Claudin-2 may be a new target for lung cancer therapy...
  45. doi request reprint Protective roles of aldo-keto reductase 1B10 and autophagy against toxicity induced by p-quinone metabolites of tert-butylhydroquinone in lung cancer A549 cells
    Satoshi Endo
    Laboratory of Biochemistry, Gifu Pharmaceutical University, Gifu 501 1196, Japan Electronic address
    Chem Biol Interact 234:282-9. 2015
    ..The data provides evidence for the first time that autophagy and AKR1B10 contribute to the defense system against the cytotoxicity caused by the electrophilic p-quinone metabolites of BHQ. ..
  46. doi request reprint Synthesis of non-prenyl analogues of baccharin as selective and potent inhibitors for aldo-keto reductase 1C3
    Satoshi Endo
    Gifu Pharmaceutical University, Gifu 501 1196, Japan Electronic address
    Bioorg Med Chem 22:5220-33. 2014
    ..4 nM) and selectivity comparable to baccharin. Additionally, 14 significantly decreased the cellular metabolism of androsterone and cytotoxic 4-oxo-2-nonenal by AKR1C3 at much lower concentrations than baccharin...
  47. doi request reprint Cloning and characterization of four rabbit aldo-keto reductases featuring broad substrate specificity for xenobiotic and endogenous carbonyl compounds: relationship with multiple forms of drug ketone reductases
    Satoshi Endo
    Gifu Pharmaceutical University, Gifu, Japan S E, T M, Y A, A I Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa, Japan K T Monash Institute of Pharmaceutical Sciences, Monash University, Victoria, Australia O E Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka, Japan S Y and Faculty of Engineering A H, Y K, Gifu University, Gifu, Japan
    Drug Metab Dispos 42:803-12. 2014
    ..Thus, the four enzymes correspond to the multiple drug ketone reductases, and may function in the metabolisms of steroids, isatin and reactive carbonyl compounds, and bile acid synthesis. ..
  48. doi request reprint Selective inhibition of human type-5 17β-hydroxysteroid dehydrogenase (AKR1C3) by baccharin, a component of Brazilian propolis
    Satoshi Endo
    Laboratory of Biochemistry, Gifu Pharmaceutical University, Gifu 501 1196, Japan
    J Nat Prod 75:716-21. 2012
    ..Additionally, 1 suppressed the proliferation of PC3 prostatic cancer cells stimulated by AKR1C3 overexpression. This study is the first demonstration that 1 is a highly selective inhibitor of AKR1C3...
  49. pmc Quercetin Decreases Claudin-2 Expression Mediated by Up-Regulation of microRNA miR-16 in Lung Adenocarcinoma A549 Cells
    Hiroyuki Sonoki
    Laboratory of Biochemistry, Department of Biopharmaceutical Sciences, Gifu Pharmaceutical University, Gifu 501 1196, Japan
    Nutrients 7:4578-92. 2015
    ..These results suggest that quercetin decreases claudin-2 expression mediated by up-regulation of miR-16 expression and instability of claudin-2 mRNA in lung adenocarcinoma cells. ..
  50. pmc Hypotonic Stress-induced Down-regulation of Claudin-1 and -2 Mediated by Dephosphorylation and Clathrin-dependent Endocytosis in Renal Tubular Epithelial Cells
    Naoko Fujii
    From the Laboratory of Biochemistry, Department of Biopharmaceutical Sciences, Gifu Pharmaceutical University, Gifu 501 1196
    J Biol Chem 291:24787-24799. 2016
    ..We suggest that hypotonic stress induces dephosphorylation, clathrin-dependent endocytosis, and degradation of claudin-1 and -2 in lysosomes, resulting in disruption of the TJ barrier in renal tubular epithelial cells...
  51. doi request reprint Claudin-18 inhibits cell proliferation and motility mediated by inhibition of phosphorylation of PDK1 and Akt in human lung adenocarcinoma A549 cells
    Shun Shimobaba
    Laboratory of Biochemistry, Department of Biopharmaceutical Sciences, Gifu Pharmaceutical University, Japan
    Biochim Biophys Acta 1863:1170-8. 2016
    ..We suggest that claudin-18 suppresses the abnormal proliferation and motility of lung epithelial cells mediated by inhibition of the PI3K/PDK1/Akt signaling pathway. ..
  52. ncbi request reprint Roles of aldo-keto reductases 1B10 and 1C3 and ATP-binding cassette transporter in docetaxel tolerance
    Toshiyuki Matsunaga
    a Laboratory of Biochemistry, Gifu Pharmaceutical University, Gifu, Japan
    Free Radic Res 50:1296-1308. 2016
    ..The results suggest that combined treatment with AKRs (1B10 and 1C3) and ABCB1 inhibitors exerts overcoming effect against the cancer resistance to DTX and cisplatin, and can be used as the adjuvant therapy...
  53. doi request reprint Up-Regulation of Transient Receptor Potential Melastatin 6 Channel Expression by Tumor Necrosis Factor-α in the Presence of Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitor
    Chisa Furukawa
    From the Laboratory of Biochemistry, Department of Biopharmaceutical Sciences, Gifu Pharmaceutical University, Gifu, Japan
    J Cell Physiol . 2016
    ..These results suggest that TNF-α reverses the reduction in Mg2+ reabsorption caused by anti-EGFR drugs. This article is protected by copyright. All rights reserved...
  54. doi request reprint Hyperosmolarity-Induced Down-Regulation of Claudin-2 Mediated by Decrease in PKCβ-Dependent GATA-2 in MDCK Cells
    Akira Ikari
    Laboratory of Biochemistry, Department of Biopharmaceutical Sciences, Gifu Pharmaceutical University, 1 25 4 Daigaku nishi, Gifu, Japan
    J Cell Physiol 230:2776-87. 2015
    ..These results suggest that hyperosmolarity decreases the expression level of claudin-2 via a decrease in PKCβ-dependent GATA-2 transcriptional activity in renal tubular epithelial cells...
  55. ncbi request reprint Probing AKR1C30 and AKR1C31 with site-directed mutagenesis: identifying the roles of residues 54 and 56 in the binding of substrates and inhibitors
    Satoshi Endo
    Laboratory of Biochemistry, Gifu Pharmaceutical University
    Biol Pharm Bull 37:1848-52. 2014
    ..The R56Q mutation decreased the affinity for only carbenoxolone among the substrates and inhibitors. Thus, the difference in the properties between the two enzymes can be attributed to their residue difference at positions 54 and 56...
  56. pmc Down-Regulation of Claudin-2 Expression and Proliferation by Epigenetic Inhibitors in Human Lung Adenocarcinoma A549 Cells
    Asami Hichino
    Gifu Pharmaceutical University, Japan
    J Biol Chem . 2017
    ..We suggest that epigenetic inhibitors suppress the abnormal proliferation of lung adenocarcinoma cells highly expressing claudin-2...
  57. doi request reprint Identification of a determinant for strict NADP(H)-specificity and high sensitivity to mixed-type steroid inhibitor of rabbit aldo-keto reductase 1C33 by site-directed mutagenesis
    Satoshi Endo
    Laboratory of Biochemistry, Gifu Pharmaceutical University, Gifu, Japan Electronic address
    Arch Biochem Biophys 569:19-25. 2015
    ..The results indicate the important role of F217 in the strict NADPH-dependency, as well as its involvement in the unique catalytic properties of AKR1C33...
  58. ncbi request reprint Enzymatic properties of a member (AKR1C20) of the aldo-keto reductase family
    Kengo Matsumoto
    Laboratory of Biochemistry, Gifu Pharmaceutical University, Mitahora Higashi, Japan
    Biol Pharm Bull 29:539-42. 2006
    ..Thus, AKR1C20 is a novel 3alpha(17beta)-HSD, which may also function as a reductase for xenobiotic alpha-dicarbonyl compounds...
  59. doi request reprint Claudin-5, -7, and -18 suppress proliferation mediated by inhibition of phosphorylation of Akt in human lung squamous cell carcinoma
    Risa Akizuki
    Laboratory of Biochemistry, Department of Biopharmaceutical Sciences, Gifu Pharmaceutical University
    Biochim Biophys Acta 1864:293-302. 2016
    ....
  60. doi request reprint Inhibition of aldo-keto reductase family 1 member B10 by unsaturated fatty acids
    Akira Hara
    Faculty of Engineering, Gifu University, Gifu, 501 1193, Japan
    Arch Biochem Biophys 609:69-76. 2016
    ..Thus, the cis-unsaturated fatty acids may be used as an adjuvant therapy for treatment of cancers that up-regulate AKR1B10...
  61. doi request reprint Human dehydrogenase/reductase (SDR family) member 11 is a novel type of 17β-hydroxysteroid dehydrogenase
    Satoshi Endo
    Laboratory of Biochemistry, Gifu Pharmaceutical University, Gifu 501 1196, Japan Electronic address
    Biochem Biophys Res Commun 472:231-6. 2016
    ..Thus, DHRS11 represents a novel type of 17β-hydroxysteroid dehydrogenase with unique catalytic properties and tissue distribution...
  62. doi request reprint Structure-activity relationship of flavonoids as potent inhibitors of carbonyl reductase 1 (CBR1)
    Yuki Arai
    Laboratory of Biochemistry, Gifu Pharmaceutical University, Gifu 501 1196, Japan
    Fitoterapia 101:51-6. 2015
    ....
  63. doi request reprint Nuclear distribution of claudin-2 increases cell proliferation in human lung adenocarcinoma cells
    Akira Ikari
    Laboratory of Biochemistry, Department of Biopharmaceutical Sciences, Gifu Pharmaceutical University, Gifu, Japan School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, Japan Electronic address
    Biochim Biophys Acta 1843:2079-88. 2014
    ..We suggest that nuclear distribution of claudin-2 is up-regulated by dephosphorylation and claudin-2 serves to retain ZONAB and cyclin D1 in the nucleus, resulting in the enhancement of cell proliferation in lung adenocarcinoma cells. ..
  64. pmc Tight junctional localization of claudin-16 is regulated by syntaxin 8 in renal tubular epithelial cells
    Akira Ikari
    From the Laboratory of Biochemistry, Department of Biopharmaceutical Sciences, Gifu Pharmaceutical University, Gifu 501 1196, Japan
    J Biol Chem 289:13112-23. 2014
    ..These results suggest that STX8 mediates the recycling of CLDN16 and constitutes an important component of the CLDN16 trafficking machinery in the kidney. ..
  65. ncbi request reprint Multiplicity of mammalian reductases for xenobiotic carbonyl compounds
    Toshiyuki Matsunaga
    Laboratory of Biochemistry, Gifu Pharmaceutical University, Japan
    Drug Metab Pharmacokinet 21:1-18. 2006
    ..Characterization of the additional enzymes suggested their involvement in species-specific biological events and species differences in the metabolism of xenobiotic carbonyl compounds...
  66. ncbi request reprint Ceramide-induced intracellular oxidant formation, iron signaling, and apoptosis in endothelial cells: protective role of endogenous nitric oxide
    Toshiyuki Matsunaga
    Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    J Biol Chem 279:28614-24. 2004
    ..We conclude that C(2)-cer-induced H(2)O(2) and TfR-dependent iron signaling are responsible for its prooxidant and proapoptotic effects and that .NO exerts an antioxidative and cytoprotective role...
  67. ncbi request reprint Electrophoretically unique amylases in rat livers: phylogenic and ontogenic study on the mammalian liver
    Iwao Koyama
    Department of Medical Technology, Junior College, Saitama Medical School, Japan
    Electrophoresis 23:3278-83. 2002
    ..These results suggest that the liver may express an etopic amylase in a certain condition...
  68. ncbi request reprint Glycated high-density lipoprotein regulates reactive oxygen species and reactive nitrogen species in endothelial cells
    Toshiyuki Matsunaga
    Department of Biochemistry, Saitama Medical School, Moroyama, Saitama, Japan
    Metabolism 52:42-9. 2003
    ..Taking all of the above findings together, gly-ox-HDL may lead to the deterioration of vascular function through altered production of reactive oxygen species and reactive nitrogen species in endothelial cells...
  69. ncbi request reprint Rabbit liver dCMP phosphohydrolase: a pyrimidine 5'-nucleotidase I-like enzyme in non-erythrocytic cells
    Shigeru Hokari
    Department of Biochemistry, Saitama Medical School, 38 Morohongo, Moroyama machi, Iruma gun, Saitama 350 0495, Japan
    Comp Biochem Physiol B Biochem Mol Biol 134:381-8. 2003
    ..3 mM, the optimal pH is approximately 7, and the enzyme requires Mg(2+). This nucleotidase may contribute to the regulation of intracellular pyrimidine nucleotides in the rabbit...
  70. ncbi request reprint Expression of alpha-amylase isozymes in rat tissues
    Shigeru Hokari
    Department of Biochemistry, Saitama Medical School, 38 Morohongo, Moroyama machi, Iruma gun, Saitama 350 0495, Japan
    Comp Biochem Physiol B Biochem Mol Biol 135:63-9. 2003
    ..Immunohistochemical analysis also indicated that the amylase proteins were specifically present in epithelial cells of rat intestinal mucosa. This is a convenient method for identification of alpha-amylase isozyme mRNA in rodent tissues...
  71. ncbi request reprint Structures of dimeric dihydrodiol dehydrogenase apoenzyme and inhibitor complex: probing the subunit interface with site-directed mutagenesis
    Vincenzo Carbone
    Department of Medicinal Chemistry, Victorian College of Pharmacy, Monash University, Parkville, Victoria 3052, Australia
    Proteins 70:176-87. 2008
    ....
  72. ncbi request reprint Caspase 3 activation in nasal capillary in patients with epistaxis
    Hiroto Nakada
    Department of Otorhinolaryngology, Saitama Medical School, 38 Morohongo, Moroyama, Iruma gun, Saitama 350 0495, Japan
    Otolaryngol Head Neck Surg 128:632-9. 2003
    ..We investigated whether an apoptosis of nasal microvessels contributes to probable mechanism of the onset of epistaxis...
  73. ncbi request reprint NF-kappa B activation in endothelial cells treated with oxidized high-density lipoprotein
    Toshiyuki Matsunaga
    Department of Biochemistry, Saitama Medical School, 38 Morohongo, Moroyama, Iruma gun, Saitama 350 0495, Japan
    Biochem Biophys Res Commun 303:313-9. 2003
    ..Taking all of the above findings together, ox-HDL activates NF-kappa B via binding to LOX-1 on the cell surface, followed by enhancement of intracellular ROS production in endothelial cells...
  74. ncbi request reprint Characterization of the epitopes specific for the monoclonal antibody 9F5-3a and quantification of oxidized HDL in human plasma
    Takanori Nakajima
    Department of Biochemistry, Saitama Medical School, 38 Morohongo, Moroyama, Saitama 350 0495, Japan
    Ann Clin Biochem 41:309-15. 2004
    ..We previously isolated a monoclonal antibody, 9F5-3a, that is specific for HDL oxidized by CuSO(4)...
  75. ncbi request reprint Ambroxol reduces LPS toxicity mediated by induction of alkaline phosphatases in rat lung
    Iwao Koyama
    Department of Medical Technology, Junior College, Saitama Medical School, Moroyama, Iruma, Saitama, Japan
    Clin Biochem 37:688-93. 2004
    ..Maintenance of high AP activity level in the lung suggests APs to have physiological significant effects against the inflammatory events induced by LPS...
  76. ncbi request reprint Alkaline phosphatases reduce toxicity of lipopolysaccharides in vivo and in vitro through dephosphorylation
    Iwao Koyama
    Department of Medical Technology, Junior College, Saitama Medical School, 38 Morohongo, Moroyama, Iruma gun, Saitama 350 0495, Japan
    Clin Biochem 35:455-61. 2002
    ..0 and 8.0, the 50% viability was at 2.0 microg/ml of LPS. These results strongly suggest that the APs reduced the toxicity of LPS, as a host defense factor against LPS...
  77. ncbi request reprint Changes in receptor activator of nuclear factor-kappaB, and its ligand, osteoprotegerin, bone-type alkaline phosphatase, and tartrate-resistant acid phosphatase in ovariectomized rats
    Takashi Miyazaki
    Department of Biochemistry, Saitama Medical School, 38 Morohongo Moroyama machi, Iruma gun, Saitama 350 0495, Japan
    J Cell Biochem 93:503-12. 2004
    ..Our results suggest that not only osteoclastogenesis accelerated but also osteoblastogenesis transiently increased during the early phase of osteoporosis...
  78. pmc Autoantibody response to microsomal epoxide hydrolase in hepatitis C and A
    Toshitaka Akatsuka
    Department of Microbiology, Saitama Medical University, Moroyama cho, Iruma gun, Saitama 350 0495, Japan
    J Autoimmun 28:7-18. 2007
    ....
  79. ncbi request reprint Characterization of four monoclonal antibodies to recombinant human tartrate-resistant acid phosphatase
    Takashi Miyazaki
    Department of Biochemistry, Saitama Medical School, 38 Morohongo, Moroyama, Iruma gun, Saitama 350 0495, Japan
    Hybrid Hybridomics 21:191-5. 2002
    ..They will certainly be of use for the study of biosynthesis, regulation and function of the TRAP enzyme...