Kazuo Kuwata

Summary

Affiliation: Gifu University
Country: Japan

Publications

  1. ncbi request reprint [Structural medicine and structure-based drug design for prion diseases]
    Kazuo Kuwata
    Tanpakushitsu Kakusan Koso 47:1292-8. 2002
  2. pmc NMR-detected hydrogen exchange and molecular dynamics simulations provide structural insight into fibril formation of prion protein fragment 106-126
    Kazuo Kuwata
    Department of Biochemistry and Biophysics, School of Medicine, Gifu University, 40 Tsukasa Machi, Gifu 500 8705, Japan
    Proc Natl Acad Sci U S A 100:14790-5. 2003
  3. ncbi request reprint Modeling of a propagation mechanism of infectious prion protein; a hexamer as the minimum infectious unit
    Hironori K Nakamura
    Division of Prion Research, Center for Emerging Infectious Diseases, Gifu University, 1 1 Yanagido, Gifu 501 1194, Japan
    Biochem Biophys Res Commun 361:789-93. 2007
  4. ncbi request reprint [Non-commutative geometrical drug discovery--the principle of geometrical regulation]
    Kazuo Kuwata
    United Graduate School of Drug Discovery and Medical Information Sciences, Gifu University, Japan
    Yakugaku Zasshi 132:873-9. 2012
  5. pmc Hot spots in prion protein for pathogenic conversion
    Kazuo Kuwata
    Center for Emerging Infectious Diseases, Department of Gene and Development, Graduate School of Medicine, Gifu University, 1 1 Yanagido, Gifu 501 1194, Japan
    Proc Natl Acad Sci U S A 104:11921-6. 2007
  6. ncbi request reprint [Rational drug discovery for prion diseases]
    Kazuo Kuwata
    Tanpakushitsu Kakusan Koso 53:727-32. 2008
  7. doi request reprint Synthesis of GN8 derivatives and evaluation of their antiprion activity in TSE-infected cells
    Tsutomu Kimura
    Center for Emerging Infectious Diseases, Gifu University, Gifu, Japan
    Bioorg Med Chem Lett 21:1502-7. 2011
  8. pmc Variety of antiprion compounds discovered through an in silico screen based on cellular-form prion protein structure: Correlation between antiprion activity and binding affinity
    Junji Hosokawa-Muto
    Center for Emerging Infectious Diseases, Gifu University, Yanagido, Japan
    Antimicrob Agents Chemother 53:765-71. 2009
  9. pmc Proper calibration of ultrasonic power enabled the quantitative analysis of the ultrasonication-induced amyloid formation process
    Kei ichi Yamaguchi
    Center for Emerging Infectious Diseases, Gifu University, Yanagido 1 1, Gifu, Japan
    Protein Sci 21:38-49. 2012
  10. doi request reprint Cold destabilization and temperature jump of the murine prion protein mPrP(23-231)
    Tomoharu Matsumoto
    Division of Prion Research, Center for Emerging Infectious Diseases, Gifu University, Yanagido 1 1, Gifu 501 1194, Japan
    Biochim Biophys Acta 1794:669-73. 2009

Collaborators

Detail Information

Publications30

  1. ncbi request reprint [Structural medicine and structure-based drug design for prion diseases]
    Kazuo Kuwata
    Tanpakushitsu Kakusan Koso 47:1292-8. 2002
  2. pmc NMR-detected hydrogen exchange and molecular dynamics simulations provide structural insight into fibril formation of prion protein fragment 106-126
    Kazuo Kuwata
    Department of Biochemistry and Biophysics, School of Medicine, Gifu University, 40 Tsukasa Machi, Gifu 500 8705, Japan
    Proc Natl Acad Sci U S A 100:14790-5. 2003
    ..Fibril formation involving polyalanine stacking is consistent with the experimental observations...
  3. ncbi request reprint Modeling of a propagation mechanism of infectious prion protein; a hexamer as the minimum infectious unit
    Hironori K Nakamura
    Division of Prion Research, Center for Emerging Infectious Diseases, Gifu University, 1 1 Yanagido, Gifu 501 1194, Japan
    Biochem Biophys Res Commun 361:789-93. 2007
    ..Dimers and trimers were rarely observed. Then, we propose a new PrP(Sc) propagation mechanism where a hexamer plays an essential role as a minimum infectious unit...
  4. ncbi request reprint [Non-commutative geometrical drug discovery--the principle of geometrical regulation]
    Kazuo Kuwata
    United Graduate School of Drug Discovery and Medical Information Sciences, Gifu University, Japan
    Yakugaku Zasshi 132:873-9. 2012
    ..For example, QC model was applied to the optimization of the chemical structure of anti-prion lead compound GN8. Arithmetic geometrical representation of these algebraic models is in progress...
  5. pmc Hot spots in prion protein for pathogenic conversion
    Kazuo Kuwata
    Center for Emerging Infectious Diseases, Department of Gene and Development, Graduate School of Medicine, Gifu University, 1 1 Yanagido, Gifu 501 1194, Japan
    Proc Natl Acad Sci U S A 104:11921-6. 2007
    ..Dynamics-based drug discovery strategy, demonstrated here focusing on the hot spots of PrP(C), will open the way to the development of novel anti-prion drugs...
  6. ncbi request reprint [Rational drug discovery for prion diseases]
    Kazuo Kuwata
    Tanpakushitsu Kakusan Koso 53:727-32. 2008
  7. doi request reprint Synthesis of GN8 derivatives and evaluation of their antiprion activity in TSE-infected cells
    Tsutomu Kimura
    Center for Emerging Infectious Diseases, Gifu University, Gifu, Japan
    Bioorg Med Chem Lett 21:1502-7. 2011
    ..51-0.83 μM. Conformational analysis of model compounds suggested that the introduction of the substituent at the benzylic position restricted the conformational variability of the diphenylmethane unit...
  8. pmc Variety of antiprion compounds discovered through an in silico screen based on cellular-form prion protein structure: Correlation between antiprion activity and binding affinity
    Junji Hosokawa-Muto
    Center for Emerging Infectious Diseases, Gifu University, Yanagido, Japan
    Antimicrob Agents Chemother 53:765-71. 2009
    ..The appropriate categorization of these diverse compounds would facilitate antiprion drug discovery and help to elucidate the pathogenic conversion mechanism...
  9. pmc Proper calibration of ultrasonic power enabled the quantitative analysis of the ultrasonication-induced amyloid formation process
    Kei ichi Yamaguchi
    Center for Emerging Infectious Diseases, Gifu University, Yanagido 1 1, Gifu, Japan
    Protein Sci 21:38-49. 2012
    ....
  10. doi request reprint Cold destabilization and temperature jump of the murine prion protein mPrP(23-231)
    Tomoharu Matsumoto
    Division of Prion Research, Center for Emerging Infectious Diseases, Gifu University, Yanagido 1 1, Gifu 501 1194, Japan
    Biochim Biophys Acta 1794:669-73. 2009
    ..The conformational instability and low barriers between different conformers may explain the unusual flexibility leading to the pathogenic conversion and the strain diversity...
  11. doi request reprint Respiratory and cardiovascular toxicity studies of a novel antiprion compound, GN8, in rats and dogs
    Junji Hosokawa-Muto
    Center for Emerging Infectious Diseases, Gifu University, Gifu, Japan
    Drug Chem Toxicol 35:264-71. 2012
    ..3 mg/kg, we did not find severe adverse effects of GN8 at doses sufficient for antiprion activity. This study would serve as a stepping stone to a clinical application of GN8 as an antiprion agent...
  12. doi request reprint Critical region for amyloid fibril formation of mouse prion protein: unusual amyloidogenic properties of the helix 2 peptide
    Kei ichi Yamaguchi
    Center for Emerging Infectious Diseases, Gifu University, Yanagido 1 1, Gifu 501 1194, Japan
    Biochemistry 47:13242-51. 2008
    ..As a whole, the alpha-helix 2 region would be crucial for the nucleation-dependent replication process of the prion protein...
  13. ncbi request reprint Molecular mechanism of a temperature-sensitive phenotype in peroxisomal biogenesis disorder
    Kazuyuki Hashimoto
    Department of Pediatrics, Gifu University School of Medicine, Gifu 501 1194, Japan
    Pediatr Res 58:263-9. 2005
    ..Structural analyses of the protein in the other genetic diseases could provide an avenue for better understanding of genotype-phenotype correlations...
  14. ncbi request reprint Strange kinetic phase in the extremely early folding process of beta-lactoglobulin
    Yuji O Kamatari
    Division of Prion Research, Center for Emerging Infectious Diseases, Gifu University, Gifu 501 1194, Japan
    FEBS Lett 581:4463-7. 2007
    ..This process can be explained by conformational shift occurring within the unfolded ensemble (U-->U'), which is followed by the non-native intermediate (I) formation of this protein...
  15. doi request reprint Theoretical study of the prion protein based on the fragment molecular orbital method
    Takeshi Ishikawa
    Division of Prion Research, Center for Emerging Infectious Diseases, Gifu University, 1 1 Yanagido, Gifu 501 1194, Japan
    J Comput Chem 30:2594-601. 2009
    ..The present FMO calculations were performed using an original program developed in our laboratory, called "Parallelized ab initio calculation system based on FMO (PAICS)"...
  16. doi request reprint Structure-based discovery of anti-influenza virus A compounds among medicines
    Mayuko Fukuoka
    United Graduate School of Drug Discovery and Medical Information Sciences, Gifu University, 1 1Yanagido, Gifu 501 1193, Japan
    Biochim Biophys Acta 1820:90-5. 2012
    ..Thus the development of anti-IAV drugs with a novel action mechanism may be an urgent theme...
  17. doi request reprint Synthesis of 9-substituted 2,3,4,9-tetrahydro-1H-carbazole derivatives and evaluation of their anti-prion activity in TSE-infected cells
    Tsutomu Kimura
    Center for Emerging Infectious Diseases, Gifu University, 1 1 Yanagido, Gifu 501 1194, Japan
    Eur J Med Chem 46:5675-9. 2011
    ..The derivatives bearing an N-ortho-halobenzyl group exhibited an improved activity, and the most potent derivative was 8 times as effective as the original lead compound, GJP14...
  18. doi request reprint Critical role of CD44 in hepatotoxin-mediated liver injury
    Kiminori Kimura
    Department of Immunotherapeutics Medinet, Graduate School of Medicine, The University of Tokyo, 1 3 7 Hongo, Bunkyo ku, Tokyo 113 8655, Japan
    J Hepatol 48:952-61. 2008
    ..Blocking of adhesion molecules is considered to be one of the therapeutic strategies inflammatory diseases, although it remains unclear whether this strategy is beneficial...
  19. ncbi request reprint An emerging concept of biomolecular dynamics and function: applications of NMR & MRI
    Kazuo Kuwata
    Department of Physiology, School of Medicine, Gifu University, Japan
    Magn Reson Med Sci 1:27-31. 2002
    ..quot; Relevant NMR techniques developed recently may also have useful application to MRI, since the critical time scale of various reactions in a living system is also around micro- to milliseconds...
  20. doi request reprint Difference in redox behaviors between copper-binding octarepeat and nonoctarepeat sites in prion protein
    Norifumi Yamamoto
    Division of Prion Research, Center for Emerging Infectious Diseases, Gifu University, Gifu 501 1194, Japan
    J Biol Inorg Chem 14:1209-18. 2009
    ..It is possible that such distinct redox activities of a copper-binding PrP are involved in the mechanism underlying prion diseases...
  21. ncbi request reprint Slow conformational dynamics in the hamster prion protein
    Kazuo Kuwata
    Department of Biochemistry and Biophysics, School of Medicine, Gifu University, Gifu 500 8705 Japan
    Biochemistry 43:4439-46. 2004
    ..These observations suggest that both the CMPG relaxation and the pressure shifts reflect slow conformational fluctuations and that these slow motions in PrP(C) are related to the trajectories leading to the transition to PrP*...
  22. pmc Pathogenic role of B cells in anti-CD40-induced necroinflammatory liver disease
    Kiminori Kimura
    First Department of Internal Medicine, Gifu University School of Medicine, Gifu, Japan
    Am J Pathol 168:786-95. 2006
    ..In conclusion, these results indicate that CD40 ligation can trigger a B-cell-mediated inflammatory response that can have pathogenic consequences for the liver...
  23. pmc Characterizing antiprion compounds based on their binding properties to prion proteins: implications as medical chaperones
    Yuji O Kamatari
    Life Science Research Center, Gifu University, Gifu 501 1194, Japan
    Protein Sci 22:22-34. 2013
    ..This proposed antiprion mechanisms of diverse antiprion compounds could help to elucidate their antiprion activities and facilitate effective antiprion drug discovery...
  24. doi request reprint Comparative 1H NMR studies on the structural looseness of the aged (A) and non-aged (N) bovine mercaptalbumin in the alkaline region
    Seiichi Era
    Department of Physiology and Biophysics, Gifu University Graduate School of Medicine, Gifu 501 1194, Japan
    Int J Biol Macromol 44:37-42. 2009
    ..At pD 9.3 in 0.10 M NaCl, the TIS values for the B*-form exhibited elongation and those for the B-form did not, indicating the presence of structural looseness in only the B*-form resulting in (A)/(N) approximately 0...
  25. ncbi request reprint Locally disordered conformer of the hamster prion protein: a crucial intermediate to PrPSc?
    Kazuo Kuwata
    Department of Biochemistry and Biophysics, School of Medicine, Gifu University, 40 Tsukasa Machi, Gifu 500 8705, Japan
    Biochemistry 41:12277-83. 2002
    ..The structural characteristics of this metastable conformer are consistent with available immunological and pathological information about the prion protein...
  26. ncbi request reprint Novel anti-cancer compounds: structure-based discovery of chemical chaperons for p53
    Yumiko Okuda
    Division of Prion Research, Center for Emerging Infectious Diseases, Graduate School of Medicine, Gifu University, Gifu 501 1194, Japan
    Oncol Rep 22:739-44. 2009
    ..In conclusion, p53 is an appropriate target for the rational design of the chemical chaperon for cancer treatment...
  27. doi request reprint Synthesis of an (11) C-Labeled Antiprion GN8 Derivative and Evaluation of Its Brain Uptake by Positron Emission Tomography
    Tsutomu Kimura
    Center for Emerging Infectious Diseases, United Graduate School of Drug Discovery and Medical Information Sciences, Gifu University, 1 1 Yanagido, Gifu 501 1194 Japan
    ChemMedChem 8:1035-9. 2013
    ....
  28. pmc Role of macrophage migration inhibitory factor in hepatitis B virus-specific cytotoxic-T-lymphocyte-induced liver injury
    Kiminori Kimura
    First Department of Internal Medicine, Gifu University School of Medicine, 1 1 Yanagido, Gifu shi Gifu 501 1194, Japan
    Clin Vaccine Immunol 13:415-9. 2006
    ..Here, we found that anti-mouse MIF antibody treatment reduced liver injury and inflammatory cell infiltration into the liver after injection of antigen-specific cytotoxic T lymphocytes into hepatitis B virus transgenic mice...
  29. ncbi request reprint Species-specific differences in the intermediate states of human and Syrian hamster prion protein detected by high pressure NMR spectroscopy
    Werner Kremer
    Institute for Biophysics and Physical Biochemistry, University of Regensburg, D 93040 Regensburg, Germany
    J Biol Chem 282:22689-98. 2007
    ..The results point to the notion that excited state conformers are important for infection and that their structural differences may crucially determine species barriers for infection...
  30. ncbi request reprint Development of a high-level expression system for deuterium-labeled human serum albumin
    Mihoko Tomida
    Department of Biochemistry, Gifu University School of Medicine, Gifu, 500 8705 Japan
    Jpn J Physiol 53:65-9. 2003
    ....