Masaru Ihira

Summary

Affiliation: Fujita Health University
Country: Japan

Publications

  1. doi request reprint Cycling probe technology to quantify and discriminate between wild-type varicella-zoster virus and Oka vaccine strains
    Masaru Ihira
    Faculty of Clinical Engineering, Fujita Health University School of Health Sciences, Toyoake, Aichi, Japan Electronic address
    J Virol Methods 193:308-13. 2013
  2. ncbi request reprint Direct detection of human herpesvirus 6 DNA in serum by variant specific loop-mediated isothermal amplification in hematopoietic stem cell transplant recipients
    Masaru Ihira
    Faculty of Clinical Engineering, Fujita Health University School of Health Sciences, Toyoake, Aichi 4701192, Japan
    J Virol Methods 167:103-6. 2010
  3. ncbi request reprint Development of the loop-mediated isothermal amplification method for rapid detection of cytomegalovirus DNA
    Ryota Suzuki
    Division of Pharmacy, Fujita Health University Hospital, Toyoake, Aichi, Japan
    J Virol Methods 132:216-21. 2006
  4. ncbi request reprint Direct detection of human herpesvirus 6 DNA in serum by the loop-mediated isothermal amplification method
    Masaru Ihira
    Department of Medical Information Technology, Fujita Health University College, Toyoake, Aichi, Japan
    J Clin Virol 39:22-6. 2007
  5. doi request reprint Elevated serum cytokine levels are associated with human herpesvirus 6 reactivation in hematopoietic stem cell transplantation recipients
    Ayano Fujita
    Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi 4701192, Japan
    J Infect 57:241-8. 2008
  6. pmc Discriminating between varicella-zoster virus vaccine and wild-type strains by loop-mediated isothermal amplification
    Yuki Higashimoto
    Department of Clinical Immunology, Fujita Health University School of Health Sciences, Toyoake, Aichi, Japan
    J Clin Microbiol 46:2665-70. 2008
  7. pmc Rapid diagnosis of human herpesvirus 6 infection by a novel DNA amplification method, loop-mediated isothermal amplification
    Masaru Ihira
    Department of Medical Information Technology, Fujita Health University College, Konan, Aichi, Japan
    J Clin Microbiol 42:140-5. 2004
  8. doi request reprint Development of quantitative RT-PCR assays for detection of three classes of HHV-6B gene transcripts
    Masaru Ihira
    Faculty of Clinical Engineering, Fujita Health University School of Health Sciences, Toyoake, Aichi, Japan
    J Med Virol 84:1388-95. 2012
  9. ncbi request reprint Reactivation of human herpesvirus 6 and 7 in pregnant women
    Masahiro Ohashi
    Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi, Japan
    J Med Virol 67:354-8. 2002
  10. pmc Detection of human herpesvirus 7 DNA by loop-mediated isothermal amplification
    Tetsushi Yoshikawa
    Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi, Japan
    J Clin Microbiol 42:1348-52. 2004

Collaborators

Detail Information

Publications38

  1. doi request reprint Cycling probe technology to quantify and discriminate between wild-type varicella-zoster virus and Oka vaccine strains
    Masaru Ihira
    Faculty of Clinical Engineering, Fujita Health University School of Health Sciences, Toyoake, Aichi, Japan Electronic address
    J Virol Methods 193:308-13. 2013
    ..The cycling probe real-time PCR technology is a reliable tool for differentiating between wild-type VZV and vOka strains in clinical samples. ..
  2. ncbi request reprint Direct detection of human herpesvirus 6 DNA in serum by variant specific loop-mediated isothermal amplification in hematopoietic stem cell transplant recipients
    Masaru Ihira
    Faculty of Clinical Engineering, Fujita Health University School of Health Sciences, Toyoake, Aichi 4701192, Japan
    J Virol Methods 167:103-6. 2010
    ..Initial validation analysis confirmed high specificity and sensitivity of the method. This method was shown to be highly reliable for monitoring active HHV-6 infection in hematopoietic stem cell transplant recipients...
  3. ncbi request reprint Development of the loop-mediated isothermal amplification method for rapid detection of cytomegalovirus DNA
    Ryota Suzuki
    Division of Pharmacy, Fujita Health University Hospital, Toyoake, Aichi, Japan
    J Virol Methods 132:216-21. 2006
    ..0, 98.9, 66.7, and 99.4%, respectively...
  4. ncbi request reprint Direct detection of human herpesvirus 6 DNA in serum by the loop-mediated isothermal amplification method
    Masaru Ihira
    Department of Medical Information Technology, Fujita Health University College, Toyoake, Aichi, Japan
    J Clin Virol 39:22-6. 2007
    ..The loop-mediated isothermal amplification method (LAMP) can detect viral DNA with high specificity, efficiency, and speed under isothermal conditions. LAMP requires only simple equipment that is available in hospital laboratories...
  5. doi request reprint Elevated serum cytokine levels are associated with human herpesvirus 6 reactivation in hematopoietic stem cell transplantation recipients
    Ayano Fujita
    Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi 4701192, Japan
    J Infect 57:241-8. 2008
    ..These results imply that proinflammatory cytokines, in particular IL-6 and TNF-alpha, play a role in the pathogenesis of HHV-6 reactivation after HSCT...
  6. pmc Discriminating between varicella-zoster virus vaccine and wild-type strains by loop-mediated isothermal amplification
    Yuki Higashimoto
    Department of Clinical Immunology, Fujita Health University School of Health Sciences, Toyoake, Aichi, Japan
    J Clin Microbiol 46:2665-70. 2008
    ..Wild-type VZV DNA was detected in 20 swab samples by either VR-1 VZV LAMP or VR-2 VZV LAMP. Sequence analysis confirmed the expected SNPs in the LAMP products amplified from the vOka strain and the five wild-type strains...
  7. pmc Rapid diagnosis of human herpesvirus 6 infection by a novel DNA amplification method, loop-mediated isothermal amplification
    Masaru Ihira
    Department of Medical Information Technology, Fujita Health University College, Konan, Aichi, Japan
    J Clin Microbiol 42:140-5. 2004
    ..Thus, detection of HHV-6 DNA in plasma by using this modified HHV-6 LAMP protocol is appropriate for diagnosis of active HHV-6 infection...
  8. doi request reprint Development of quantitative RT-PCR assays for detection of three classes of HHV-6B gene transcripts
    Masaru Ihira
    Faculty of Clinical Engineering, Fujita Health University School of Health Sciences, Toyoake, Aichi, Japan
    J Med Virol 84:1388-95. 2012
    ..Thus, this method could be a rapid and lower cost alternative to virus culture, which is difficult to perform generally, for identifying active HHV-6B infection...
  9. ncbi request reprint Reactivation of human herpesvirus 6 and 7 in pregnant women
    Masahiro Ohashi
    Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi, Japan
    J Med Virol 67:354-8. 2002
    ..There was no statistical association between virus shedding in the genital tract and the clinical features examined in this study...
  10. pmc Detection of human herpesvirus 7 DNA by loop-mediated isothermal amplification
    Tetsushi Yoshikawa
    Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi, Japan
    J Clin Microbiol 42:1348-52. 2004
    ..After a 60-min LAMP reaction, HHV-7 LAMP product could be detected in acute-phase plasma samples...
  11. doi request reprint Host factors associated with the kinetics of Epstein-Barr virus DNA load in patients with primary Epstein-Barr virus infection
    Hidetaka Nakai
    Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Japan
    Microbiol Immunol 56:93-8. 2012
    ..Based on this, it was concluded that the kinetics of EBV DNA load can be divided to two different patterns after primary EBV infection, and immune response might be associated with viral clearance...
  12. doi request reprint Loop-mediated isothermal amplification for discriminating between human herpesvirus 6 A and B
    Masaru Ihira
    Department of Medical Information Technology, Fujita Health University College, Toyoake, Aichi, Japan
    J Virol Methods 154:223-5. 2008
    ..HHV-6 DNA was amplified directly from the serum samples and all 20 LAMP products were positive for HHV-6 B...
  13. ncbi request reprint Human herpesvirus 6 infection in adult living related liver transplant recipients
    Masahiro Ohashi
    Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi, Japan
    Liver Transpl 14:100-9. 2008
    ..0411). Moreover, tumor necrosis factor alpha levels were also higher in recipients with HHV-6 viremia (P < 0.0001) or reactivation (P = 0.0011) than in recipients without viremia or reactivation 4 weeks post-transplant...
  14. ncbi request reprint Monitoring of active HHV-6 infection in bone marrow transplant recipients by real time PCR; comparison to detection of viral DNA in plasma by qualitative PCR
    Masaru Ihira
    Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi 470 1192, Japan
    Microbiol Immunol 46:701-5. 2002
    ..033). In patients with HHV-6 viremia, mean HHV-6 DNA copy number was higher in the samples collected at 2 weeks after BMT than the samples collected at any other time period...
  15. doi request reprint Cytokine and chemokine responses in pediatric patients with severe pneumonia associated with pandemic A/H1N1/2009 influenza virus
    Yuji Matsumoto
    Department of Pediatrics, Fujita Health University School of Medicine, Aichi, Japan
    Microbiol Immunol 56:651-5. 2012
    ..001). These cytokines and chemokines may play important roles in the pathogenesis of childhood pneumonia associated with A/H1N1/2009 influenza virus infection...
  16. ncbi request reprint Variation of human herpesvirus 7 shedding in saliva
    Masaru Ihira
    Department of Medical Information Technology, Fujita Health University College, Toyoake, Japan
    J Infect Dis 188:1352-4. 2003
    ..031) and low antibody titer (P=.006) were correlated with frequent viral shedding (5 or 6 times/6 months)...
  17. ncbi request reprint Rapid detection of varicella-zoster virus infection by a loop-mediated isothermal amplification method
    Souki Okamoto
    Department of Medicine, Division of Respiratory Medicine, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan
    J Med Virol 74:677-82. 2004
    ..Requirement of a DNA extraction step in the VZV LAMP method was examined in next experiment. The turbidity assay detected a VZV LAMP product in all of the 20 positive swab samples (samples numbers 13-32), regardless of DNA extraction...
  18. doi request reprint Different characteristics of human herpesvirus 6 encephalitis between primary infection and viral reactivation
    Yoshiki Kawamura
    Department of Pediatrics, Fujita Health University School of Medicine, 1 98 Dengakugakubo, Kutsukake cho, Toyoake, Aichi 4701192, Japan
    J Clin Virol 51:12-9. 2011
    ..Pathogenesis of human herpesvirus 6 (HHV-6) encephalitis, in particular difference between HHV-6 encephalitis at the time of primary infection and reactivation remains unclear...
  19. ncbi request reprint Comparison of loop-mediated isothermal amplification, real-time PCR, and virus isolation for the detection of herpes simplex virus in genital lesions
    Hiroko Sugiyama
    Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi 470 1192, Japan
    J Med Virol 75:583-7. 2005
    ..Therefore, the sensitivity of the LAMP method increased to about 80%...
  20. ncbi request reprint Heat denaturation increases the sensitivity of the cytomegalovirus loop-mediated isothermal amplification method
    Ryota Suzuki
    Department of Pharmacy, Faculty of Clinical Engineering, Fujita Health University School of Health Sciences, Japan
    Microbiol Immunol 54:466-70. 2010
    ..3%) by the new CMV LAMP protocol. When the pp65 antigenemia assay was used as the standard method, the sensitivity, specificity, PPV, and NPV of the new protocol were 92.9%, 77.7%, 62.2%, and 96.5%, respectively...
  21. ncbi request reprint Copy Numbers of Telomeric Repeat Sequences of Human Herpesvirus 6B in Clinical Isolates: Possibility of Mixed Infections
    Yuri Kato
    Department of Clinical Laboratory, Fujita Health University Hospital, Toyoake, Japan
    J Clin Microbiol 52:419-24. 2014
    ....
  22. ncbi request reprint Human herpesvirus 6 reactivation and inflammatory cytokine production in patients with drug-induced hypersensitivity syndrome
    Tetsushi Yoshikawa
    Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi, Japan
    J Clin Virol 37:S92-6. 2006
    ..However, mechanisms for viral reactivation and modification of the clinical features remain unclear...
  23. ncbi request reprint Evaluation of active human herpesvirus 6 infection by reverse transcription-PCR
    Tetsushi Yoshikawa
    Laboratory of Virology, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Nagoya, Aichi, Japan
    J Med Virol 70:267-72. 2003
    ..An RT-PCR assay screening for both immediate early and late genes may be useful for monitoring active HHV-6 infection. No HHV-6 reactivation was found in patients with hypercytokinemia using the RT-PCR assay...
  24. pmc Development of a human herpesvirus 6 species-specific immunoblotting assay
    Yuki Higashimoto
    Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi, Japan
    J Clin Microbiol 50:1245-51. 2012
    ..In conclusion, this novel serological assay based on immunoblot analysis using recombinant HHV-6A p100 and HHV-6B 101K allowed us to discriminate between HHV-6A- and HHV-6B-specific antibodies...
  25. ncbi request reprint Serological examination of human herpesvirus 6 and 7 in patients with coronary artery disease
    Masaru Ihira
    Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi, Japan
    J Med Virol 67:534-7. 2002
    ..558). The present study demonstrates that HHV-6 and -7 reactivation is not associated with the establishment of coronary artery stenosis and restenosis following balloon angioplasty...
  26. ncbi request reprint Spread of varicella-zoster virus DNA to the environment from varicella patients who were treated with oral acyclovir
    Kyoko Suzuki
    Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi, Japan
    Pediatr Int 45:458-60. 2003
    ..The purpose of the present study was to determine the degree of spread of varicella-zoster virus to the environment (VZV) from varicella patients who received oral acyclovir treatment...
  27. doi request reprint Kinetics of cytokine and chemokine responses in patients with primary human herpesvirus 6 infection
    Tetsushi Yoshikawa
    Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi 4701192, Japan
    J Clin Virol 50:65-8. 2011
    ..However, basic data for cytokine and chemokine synthesis in primary HHV-6 infected patient without complication is lacking...
  28. ncbi request reprint Analysis of shedding of 3 beta-herpesviruses in saliva from patients with connective tissue diseases
    Tetsushi Yoshikawa
    Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi, Japan
    J Infect Dis 192:1530-6. 2005
    ..Whether an association exists between infection with beta -herpesviruses and connective tissue diseases remains unclear, as are the mechanisms for the regulation of these infections in the salivary glands...
  29. doi request reprint Copy Numbers of Telomeric Repeat Sequences of Human Herpesvirus 6B in Clinical Isolates: Possibility of Mixed Infections
    Yuri Kato
    Department of Clinical Laboratory, Fujita Health University Hospital, Toyoake, Japan
    J Clin Microbiol 52:419-24. 2014
    ....
  30. ncbi request reprint Hemiconvulsion-hemiplegia syndrome and primary human herpesvirus 7 infection
    Jun ichi Kawada
    Department of Pediatrics, Okazaki City Hospital, Okazaki, Aichi, Japan
    Brain Dev 26:412-4. 2004
    ..DWI are useful for the early evaluation of HH syndrome. Vascular disorders due to HHV-7 infection may have been related to the development of HH syndrome in this patient...
  31. ncbi request reprint Direct detection of human herpesvirus 6B by the LAMP method using newly developed dry-reagents
    Tetsushi Yoshikawa
    Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi, Japan Electronic address
    J Virol Methods 201:65-7. 2014
    ..The sensitivity of the original and dry-reagent methods was 10 copies/reaction and 100 copies/reaction, respectively. The dry-reagent LAMP method was highly sensitive (94.0%) and specific (96.0%) for the detection of HHV-6B. ..
  32. ncbi request reprint Dual roles for the telomeric repeats in chromosomally integrated human herpesvirus-6
    Tamae Ohye
    Division of Molecular Genetics, Institute for Comprehensive Medical Science, Fujita Health University, Toyoake, Aichi 470 1192, Japan
    Sci Rep 4:4559. 2014
    ..We conclude that the viral TRS in the DR regions fulfill dual functions for CIHHV-6: homology-mediated integration into the telomeric region of the chromosome and neo-telomere formation that is then stably transmitted...
  33. doi request reprint Clinical utility of loop-mediated isothermal amplification assay for the diagnosis of common alpha herpesvirus skin infections
    Tsukane Kobayashi
    Department of Dermatology, Fujita Health University School of Medicine, Toyoake, Japan
    J Dermatol 40:1033-7. 2013
    ..LAMP performed without DNA extraction remains sensitive; thus, LAMP represents a quick and economical method for the diagnosis of common alpha herpesvirus skin infections. ..
  34. ncbi request reprint Rapid detection of human herpesvirus 8 DNA using loop-mediated isothermal amplification
    Tomoe Kuhara
    Department of Dermatology, Aichi Medical University School of Medicine, Aichi, Nagakute, Japan
    J Virol Methods 144:79-85. 2007
    ..HHV-8 DNA was detected in 14 of the 17 Kaposi's sarcoma tissue samples and both of the primary effusion lymphoma cell lines. Viral DNA was not detected in HHV-8 LAMP-negative samples using the real-time PCR method...
  35. ncbi request reprint Human herpesvirus 7-associated meningitis and optic neuritis in a patient after allogeneic stem cell transplantation
    Tetsushi Yoshikawa
    Laboratory of Virology, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Nagoya, Japan
    J Med Virol 70:440-3. 2003
    ..Although neither HHV-6 nor cytomegalovirus (CMV) DNA was detected in CSF collected at day 41, HHV-7 DNA was detected in the sample. Viral DNA was not detected in CSF collected at day 93...
  36. ncbi request reprint Nationwide survey of rotavirus-associated encephalopathy and sudden unexpected death in Japan
    Yoshiki Kawamura
    Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi, Japan
    Brain Dev 36:601-7. 2014
    ..0 and 4.9 cases in Japan, respectively. Elevated LDH (>500IU/L) or acidemia (pH<7.15) were related to a poor prognosis of the encephalitis/encephalopathy. ..
  37. doi request reprint Cytokine and chemokine responses in the blood and cerebrospinal fluid of patients with human herpesvirus 6B-associated acute encephalopathy with biphasic seizures and late reduced diffusion
    Yoshiki Kawamura
    Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi, Japan
    J Med Virol 86:512-8. 2014
    ..Moreover, the regulation of cytokine networks appears to be different between peripheral blood (systemic) and central nervous system...
  38. ncbi request reprint Detection of herpesvirus DNA in the serum of immunocompetent children
    Shinya Hara
    Department of Pediatrics, Nagoya University School of Medicine, Aichi, Japan
    Microbiol Immunol 46:177-80. 2002
    ..When herpesvirus DNA is detected in the serum, a careful interpretation is necessary to diagnose a primary infection or a virus-associated disease...