T Notomi

Summary

Affiliation: Eiken Chemical Co
Country: Japan

Publications

  1. ncbi request reprint Loop-mediated isothermal amplification (LAMP): principle, features, and future prospects
    Tsugunori Notomi
    Biochemical Research Laboratory, Research and Development Division, Eiken Chemical Co, Ltd 1381 3 Shimoishigami, Ohtawara, Tochigi, 324 0036, Japan
    J Microbiol 53:1-5. 2015
  2. pmc Detection of Middle East respiratory syndrome coronavirus using reverse transcription loop-mediated isothermal amplification (RT-LAMP)
    Kazuya Shirato
    Laboratory of Acute Respiratory Viral Diseases and Cytokines, Department of Virology III, National Institute of Infectious Disease, Laboratory of Acute Respiratory Viral Diseases and Cytokines, 4 7 1 Gakuen, Musashimurayama, Tokyo 208 0011, Japan
    Virol J 11:139. 2014
  3. pmc Sequence specific visual detection of LAMP reactions by addition of cationic polymers
    Yasuyoshi Mori
    Eiken Chemical Co, Ltd, 1381 3 Shimoishigami, Ohtawara, Tochigi, 324 0036, Japan
    BMC Biotechnol 6:3. 2006
  4. pmc Loop-mediated isothermal amplification of DNA
    T Notomi
    Eiken Chemical Co Ltd, 1381 3 Shimoishigami, Ohtawara, Tochigi 324 0036, Japan, Department of Biochemistry and Molecular Biology, The University of Tokyo, Graduate School of Medicine, Bunkyo ku, Tokyo 113 0033, Japan
    Nucleic Acids Res 28:E63. 2000
  5. ncbi request reprint Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation
    Y Mori
    Eiken Chemical Company, Ltd, 1381 3 Shimoishigami, Ohtawara, Tochigi 324 0036, Japan
    Biochem Biophys Res Commun 289:150-4. 2001
  6. pmc Use of loop-mediated isothermal amplification of the IS900 sequence for rapid detection of cultured Mycobacterium avium subsp. paratuberculosis
    M Enosawa
    Hokkaido Animal Research Center, Shintoku Eiken Chemical Co, Ltd, Ohtawara, Japan
    J Clin Microbiol 41:4359-65. 2003
  7. ncbi request reprint Accelerated reaction by loop-mediated isothermal amplification using loop primers
    K Nagamine
    Eiken Chemical Co Ltd 1381 3 Shimoishigami, Ohtawara, Tochigi 324 0036, Japan
    Mol Cell Probes 16:223-9. 2002
  8. ncbi request reprint Loop-mediated isothermal amplification method for the rapid detection of Enterococcus faecalis in infected root canals
    H Kato
    Division of Community Oral Health Science, Kyushu Dental College, Fukuoka, Kyushu, Japan
    Oral Microbiol Immunol 22:131-5. 2007
  9. ncbi request reprint Rapid detection of Actinobacillus actinomycetemcomitans using a loop-mediated isothermal amplification method
    R Osawa
    Division of Community Oral Health Science, Kyushu Dental College, Kokurakita ku, Kitakyushu, Japan
    Oral Microbiol Immunol 22:252-9. 2007
  10. ncbi request reprint Rapid detection of the cariogenic pathogens Streptococcus mutans and Streptococcus sobrinus using loop-mediated isothermal amplification
    S Nagashima
    Division of Community Oral Health Science, Kyushu Dental College, Kitakyushu, Japan
    Oral Microbiol Immunol 22:361-8. 2007

Collaborators

Detail Information

Publications10

  1. ncbi request reprint Loop-mediated isothermal amplification (LAMP): principle, features, and future prospects
    Tsugunori Notomi
    Biochemical Research Laboratory, Research and Development Division, Eiken Chemical Co, Ltd 1381 3 Shimoishigami, Ohtawara, Tochigi, 324 0036, Japan
    J Microbiol 53:1-5. 2015
    ..The LAMP reaction has a wide range of possible applications, including point-of-care testing, genetic testing in resource-poor settings (such as in developing countries), and rapid testing of food products and environmental samples. ..
  2. pmc Detection of Middle East respiratory syndrome coronavirus using reverse transcription loop-mediated isothermal amplification (RT-LAMP)
    Kazuya Shirato
    Laboratory of Acute Respiratory Viral Diseases and Cytokines, Department of Virology III, National Institute of Infectious Disease, Laboratory of Acute Respiratory Viral Diseases and Cytokines, 4 7 1 Gakuen, Musashimurayama, Tokyo 208 0011, Japan
    Virol J 11:139. 2014
    ..Therefore, it is urgently necessary to develop as many specific genetic diagnostic methods as possible to allow stable diagnosis of MERS-CoV infections...
  3. pmc Sequence specific visual detection of LAMP reactions by addition of cationic polymers
    Yasuyoshi Mori
    Eiken Chemical Co, Ltd, 1381 3 Shimoishigami, Ohtawara, Tochigi, 324 0036, Japan
    BMC Biotechnol 6:3. 2006
    ..We have studied a new method for the sequence-specific visual detection of minute amounts of nucleic acids using precipitation reaction by addition of cationic polymers to amplicons of Loop mediated isothermal Amplification (LAMP)...
  4. pmc Loop-mediated isothermal amplification of DNA
    T Notomi
    Eiken Chemical Co Ltd, 1381 3 Shimoishigami, Ohtawara, Tochigi 324 0036, Japan, Department of Biochemistry and Molecular Biology, The University of Tokyo, Graduate School of Medicine, Bunkyo ku, Tokyo 113 0033, Japan
    Nucleic Acids Res 28:E63. 2000
    ..Because LAMP recognizes the target by six distinct sequences initially and by four distinct sequences afterwards, it is expected to amplify the target sequence with high selectivity...
  5. ncbi request reprint Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation
    Y Mori
    Eiken Chemical Company, Ltd, 1381 3 Shimoishigami, Ohtawara, Tochigi 324 0036, Japan
    Biochem Biophys Res Commun 289:150-4. 2001
    ....
  6. pmc Use of loop-mediated isothermal amplification of the IS900 sequence for rapid detection of cultured Mycobacterium avium subsp. paratuberculosis
    M Enosawa
    Hokkaido Animal Research Center, Shintoku Eiken Chemical Co, Ltd, Ohtawara, Japan
    J Clin Microbiol 41:4359-65. 2003
    ..These results indicate that LAMP can provide a rapid yet simple test for the detection of MAP...
  7. ncbi request reprint Accelerated reaction by loop-mediated isothermal amplification using loop primers
    K Nagamine
    Eiken Chemical Co Ltd 1381 3 Shimoishigami, Ohtawara, Tochigi 324 0036, Japan
    Mol Cell Probes 16:223-9. 2002
    ..Since the total time of analysis including detection is less than 1h, this new method should facilitate genetic analysis, including genetic diagnosis in the clinical laboratory...
  8. ncbi request reprint Loop-mediated isothermal amplification method for the rapid detection of Enterococcus faecalis in infected root canals
    H Kato
    Division of Community Oral Health Science, Kyushu Dental College, Fukuoka, Kyushu, Japan
    Oral Microbiol Immunol 22:131-5. 2007
    ..faecalis in clinical specimens from root canals...
  9. ncbi request reprint Rapid detection of Actinobacillus actinomycetemcomitans using a loop-mediated isothermal amplification method
    R Osawa
    Division of Community Oral Health Science, Kyushu Dental College, Kokurakita ku, Kitakyushu, Japan
    Oral Microbiol Immunol 22:252-9. 2007
    ..actinomycetemcomitans...
  10. ncbi request reprint Rapid detection of the cariogenic pathogens Streptococcus mutans and Streptococcus sobrinus using loop-mediated isothermal amplification
    S Nagashima
    Division of Community Oral Health Science, Kyushu Dental College, Kitakyushu, Japan
    Oral Microbiol Immunol 22:361-8. 2007
    ..The assay procedure is quite simple: the amplification is carried out in a single tube under isothermal conditions at 63 degrees C, and the result can be obtained in less than 1 h...