Akihiro Wada

Summary

Affiliation: Ehime University
Country: Japan

Publications

  1. ncbi Helicobacter pylori vacuolating cytotoxin, VacA, is responsible for gastric ulceration
    Akihiro Wada
    Department of Bacteriology, Institute of Tropical Medicine, Nagasaki University, Nagasaki 8528523, Japan
    J Biochem 136:741-6. 2004
  2. ncbi Gangliosides act as co-receptors for Salmonella enteritidis FliC and promote FliC induction of human beta-defensin-2 expression in Caco-2 cells
    Ken ichi Ogushi
    Department of Bacteriology, Institute of Tropical Medicine, Nagasaki University, Nagasaki 852 8523, Japan
    J Biol Chem 279:12213-9. 2004
  3. ncbi Cytotoxicity and recognition of receptor-like protein tyrosine phosphatases, RPTPalpha and RPTPbeta, by Helicobacter pylori m2VacA
    Blanquita B De Guzman
    Department of Bacteriology, Institute of Tropical Medicine, Nagasaki University, Nagasaki 8528523, Japan
    Cell Microbiol 7:1285-93. 2005
  4. ncbi Essential domain of receptor tyrosine phosphatase beta (RPTPbeta) for interaction with Helicobacter pylori vacuolating cytotoxin
    Kinnosuke Yahiro
    Department of Bacteriology, Institute of Tropical Medicine, Nagasaki University, Nagasaki 8528523, Japan
    J Biol Chem 279:51013-21. 2004
  5. pmc Clustering of Helicobacter pylori VacA in lipid rafts, mediated by its receptor, receptor-like protein tyrosine phosphatase beta, is required for intoxication in AZ-521 Cells
    Masaaki Nakayama
    Department of Bacteriology, Institute of Tropical Medicine, Nagasaki University, Nagasaki 8528523, Japan
    Infect Immun 74:6571-80. 2006
  6. ncbi High molecular weight factor in FCS inhibits Helicobacter pylori VacA-binding to its receptor, RPTPbeta, on AZ-521
    Takahiro Kimura
    Department of Bacteriology, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Nagasaki 852 8523, Japan
    Microbiol Immunol 47:105-7. 2003
  7. ncbi Protein-tyrosine phosphatase alpha, RPTP alpha, is a Helicobacter pylori VacA receptor
    Kinnosuke Yahiro
    Department of Bacteriology, Institute of Tropical Medicine, Nagasaki University, Nagasaki 8528523, Japan
    J Biol Chem 278:19183-9. 2003
  8. ncbi Helicobacter pylori VacA activates the p38/activating transcription factor 2-mediated signal pathway in AZ-521 cells
    Masaaki Nakayama
    Department of Bacteriology, Institute of Tropical Medicine, Nagasaki University, Nagasaki 8528523, Japan
    J Biol Chem 279:7024-8. 2004
  9. ncbi [Structure and intoxication of VacA]
    Toshiya Hirayama
    Department of Bacteriology, Institute of Tropical Medicine, Nagasaki University
    Nihon Rinsho 63:53-7. 2005
  10. doi Alarin but not its alternative-splicing form, GALP (Galanin-like peptide) has antimicrobial activity
    Akihiro Wada
    Department of Bacteriology, Institute of Tropical Medicine, Nagasaki University, Nagasaki 8528523, Japan Electronic address
    Biochem Biophys Res Commun 434:223-7. 2013

Collaborators

Detail Information

Publications28

  1. ncbi Helicobacter pylori vacuolating cytotoxin, VacA, is responsible for gastric ulceration
    Akihiro Wada
    Department of Bacteriology, Institute of Tropical Medicine, Nagasaki University, Nagasaki 8528523, Japan
    J Biochem 136:741-6. 2004
    ..As the potential roles of VacA as a ligand for RPTPalpha and RPTPbeta are only poor understood, further studies are needed to determine the importance of VacA in the pathogenisis of disease due to H. pylori infection...
  2. ncbi Gangliosides act as co-receptors for Salmonella enteritidis FliC and promote FliC induction of human beta-defensin-2 expression in Caco-2 cells
    Ken ichi Ogushi
    Department of Bacteriology, Institute of Tropical Medicine, Nagasaki University, Nagasaki 852 8523, Japan
    J Biol Chem 279:12213-9. 2004
    ..These results suggest that gangliosides act as co-receptors with TLR5 for FliC and promote hBD-2 expression via mitogen-activated protein kinase...
  3. ncbi Cytotoxicity and recognition of receptor-like protein tyrosine phosphatases, RPTPalpha and RPTPbeta, by Helicobacter pylori m2VacA
    Blanquita B De Guzman
    Department of Bacteriology, Institute of Tropical Medicine, Nagasaki University, Nagasaki 8528523, Japan
    Cell Microbiol 7:1285-93. 2005
    ..Different post-translational modifications of RPTPalpha in HeLa cells may be responsible for the reduced susceptibility to m2VacA...
  4. ncbi Essential domain of receptor tyrosine phosphatase beta (RPTPbeta) for interaction with Helicobacter pylori vacuolating cytotoxin
    Kinnosuke Yahiro
    Department of Bacteriology, Institute of Tropical Medicine, Nagasaki University, Nagasaki 8528523, Japan
    J Biol Chem 279:51013-21. 2004
    ....
  5. pmc Clustering of Helicobacter pylori VacA in lipid rafts, mediated by its receptor, receptor-like protein tyrosine phosphatase beta, is required for intoxication in AZ-521 Cells
    Masaaki Nakayama
    Department of Bacteriology, Institute of Tropical Medicine, Nagasaki University, Nagasaki 8528523, Japan
    Infect Immun 74:6571-80. 2006
    ..Thus, receptor-dependent translocation of VacA to lipid rafts is critical for signaling pathways leading to p38 MAP kinase/ATF-2 activation and vacuolation...
  6. ncbi High molecular weight factor in FCS inhibits Helicobacter pylori VacA-binding to its receptor, RPTPbeta, on AZ-521
    Takahiro Kimura
    Department of Bacteriology, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Nagasaki 852 8523, Japan
    Microbiol Immunol 47:105-7. 2003
    ..These data show that the high molecular weight fraction of FCS inhibits VacA action though its ability to block toxin binding to its receptor, RPTPbeta, on AZ-521 cells...
  7. ncbi Protein-tyrosine phosphatase alpha, RPTP alpha, is a Helicobacter pylori VacA receptor
    Kinnosuke Yahiro
    Department of Bacteriology, Institute of Tropical Medicine, Nagasaki University, Nagasaki 8528523, Japan
    J Biol Chem 278:19183-9. 2003
    ..These data suggest that RPTP alpha acts as a receptor for VacA in G401 cells. Thus, two receptor tyrosine phosphatases, RPTP alpha and RPTP beta, serve as VacA receptors...
  8. ncbi Helicobacter pylori VacA activates the p38/activating transcription factor 2-mediated signal pathway in AZ-521 cells
    Masaaki Nakayama
    Department of Bacteriology, Institute of Tropical Medicine, Nagasaki University, Nagasaki 8528523, Japan
    J Biol Chem 279:7024-8. 2004
    ..The cytotoxin may thus act independently on several cellular targets, leading to disruption of signaling, regulatory, and metabolic pathways...
  9. ncbi [Structure and intoxication of VacA]
    Toshiya Hirayama
    Department of Bacteriology, Institute of Tropical Medicine, Nagasaki University
    Nihon Rinsho 63:53-7. 2005
  10. doi Alarin but not its alternative-splicing form, GALP (Galanin-like peptide) has antimicrobial activity
    Akihiro Wada
    Department of Bacteriology, Institute of Tropical Medicine, Nagasaki University, Nagasaki 8528523, Japan Electronic address
    Biochem Biophys Res Commun 434:223-7. 2013
    ..coli but not the gram-positive bacteria, Staphylococcus aureus. Thus, these data suggest that alarin has potentials as an antimicrobial and should be considered for the development in human therapeutics...
  11. doi Direct binding of gangliosides to Helicobacter pylori vacuolating cytotoxin (VacA) neutralizes its toxin activity
    Akihiro Wada
    Department of Bacteriology, Nagasaki University, Nagasaki, Japan
    Glycobiology 20:668-78. 2010
    ..pylori VacA to free gangliosides neutralizes the toxin activity of VacA. These findings offer an alternative insight into the role of gangliosides in VacA toxicity and the pathogenesis of H. pylori...
  12. ncbi Helicobacter pylori vacuolating cytotoxin, VacA
    Toshiya Hirayama
    Department of Bacteriology, Institute of Tropical Medicine, Nagasaki University, Nagasaki 852 8523, Japan
    Jpn J Infect Dis 55:1-5. 2002
    ..Recent molecular and cellular studies of VacA action have begun to unravel its structure and the details of the mechanism of gastric injury caused by H. pylori infection...
  13. ncbi [Studies on biological activity of antimicrobial peptides, defensins, and the inducible mechanism in mammalian cells]
    Akihiro Wada
    Department of Bacteriology, Institute of Tropical Medicine, Nagasaki University
    Nihon Saikingaku Zasshi 58:595-602. 2003
  14. ncbi In vitro gene transfection using dendritic poly(L-lysine)
    Mio Ohsaki
    Department of Applied Chemistry, Faculty of Engineering, Nagasaki University, 852 8521, Japan
    Bioconjug Chem 13:510-7. 2002
    ..We also expect to be able to easily construct DPK-based functional gene carriers, e.g., DPKs modified by ligands such as a sugar chain, which can enable advanced gene delivery in vivo...
  15. ncbi Vacuolating cytotoxin A is associated with increased thrombin generation in gastric mucosa
    Yuko Suzuki
    Department of Internal Medicine, Division of Gastroenterology and Hepatology, Mie University School of Medicine, Edobashi 2 174, Tsu City, Mie 514 8507, Japan
    Helicobacter 10:323-31. 2005
    ..The present study was undertaken to assess in vivo the relationship between gastric intramucosal generation of thrombin and Helicobacter pylori infection...
  16. ncbi Helicobacter pylori vacuolating cytotoxin induces activation of the proapoptotic proteins Bax and Bak, leading to cytochrome c release and cell death, independent of vacuolation
    Eiki Yamasaki
    PRESTO, Japan Science and Technology Corporation, Saitama 332 0012, Japan
    J Biol Chem 281:11250-9. 2006
    ..These results suggest that VacA acts through different signaling pathways to induce apoptosis via Bax activation, independent of vacuolation...
  17. ncbi Inhibitory effects of polyphenols on gastric injury by Helicobacter pylori VacA toxin
    Kinnosuke Yahiro
    Department of Molecular Infectiology, Graduate School of Medicine, Chiba University, Chiba, Japan
    Helicobacter 10:231-9. 2005
    ..pylori-induced gastritis and ulceration. The aim of this study is to assess whether several naturally occurring polyphenols inhibit VacA activities in vitro and in vivo...
  18. ncbi Relationship between gastric ulcer and Helicobacter pylori VacA detected in gastric juice using bead-ELISA method
    Daisuke Shirasaka
    Division of Diabetes, Digestive, and Kidney Disease, Department of Clinical Molecular Medicine, Kobe University School of Medicine, Kobe, Hyogo, Japan
    Helicobacter 7:281-6. 2002
    ..However, no studies have ever tried to assay VacA produced in the human stomach. We applied a very sensitive and simple method, bead-ELISA, to detect VacA in gastric juice...
  19. ncbi Mice deficient in protein tyrosine phosphatase receptor type Z are resistant to gastric ulcer induction by VacA of Helicobacter pylori
    Akihiro Fujikawa
    Division of Molecular Neurobiology, National Institute for Basic Biology, 38 Nishigonaka, Myodaiji cho, Okazaki 444 8585, Japan
    Nat Genet 33:375-81. 2003
    ..Furthermore, pleiotrophin (PTN), an endogenous ligand of Ptprz, also induced gastritis specifically in Ptprz+/+ mice when administered orally. Taken together, these data indicate that erroneous Ptprz signaling induces gastric ulcers...
  20. ncbi Cutting edge: VacA, a vacuolating cytotoxin of Helicobacter pylori, directly activates mast cells for migration and production of proinflammatory cytokines
    Volaluck Supajatura
    Department of Dermatology, Juntendo University School of Medicine, Tokyo, Japan
    J Immunol 168:2603-7. 2002
    ..The present study suggests that early activation of mast cells by VacA may be the host early response to clear the bacteria and also may contribute to the pathogenesis of H. pylori-induced gastritis...
  21. ncbi Involvement of syntaxin 7 in human gastric epithelial cell vacuolation induced by the Helicobacter pylori-produced cytotoxin VacA
    Junko Suzuki
    Department of Gastroenterology, University of Tokyo School of Medicine, Tokyo 113 8655, Japan
    J Biol Chem 278:25585-90. 2003
    ..We suggest that syntaxin 7 is involved in the intracellular vacuolation induced by VacA...
  22. ncbi Helicobacter pylori strain-specific modulation of gastric inflammation in Mongolian gerbils
    Ken Ohnita
    Second Department of Internal Medicine, Nagasaki University School of Medicine, 1 7 1 Sakamoto, Nagasaki, Japan
    World J Gastroenterol 11:1549-53. 2005
    ..The cag pathogenicity island (PAI) is one of potential virulence determinants of Helicobacter pylori. The Mongolian gerbil is a suitable experimental animal for the screening of virulence factors of H pylori...
  23. ncbi Helicobacter pylori induces matrix metalloproteinase-9 through activation of nuclear factor kappaB
    Naoki Mori
    Department of Virology, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan
    Gastroenterology 124:983-92. 2003
    ..The aim of this study was to analyze the molecular mechanism responsible for Helicobacter pylori-mediated MMP expression...
  24. pmc Helicobacter pylori induces RANTES through activation of NF-kappa B
    Naoki Mori
    Department of Virology, Faculty of Medicine, University of the Ryukyus, Nishihara, Okinawa, Japan
    Infect Immun 71:3748-56. 2003
    ..pylori induced NF-kappa B activation through an intracellular signaling pathway that involved IKK and NIK, leading to RANTES gene transcription. RANTES induction by H. pylori may play an important role in gastric inflammation...
  25. ncbi High concentrations of human beta-defensin 2 in gastric juice of patients with Helicobacter pylori infection
    Hajime Isomoto
    Second Department of Internal Medicine, Nagasaki University School of Medicine, 1 7 1 Sakamoto, Nagasaki 852 8501, Japan
    World J Gastroenterol 11:4782-7. 2005
    ..Unlike HBD-1, the HBD-2 expression is augmented by Helicobacter pylori (H pylori). We sought to determine HBD-1 and HBD-2 concentrations in gastric juice during H pylori infection...
  26. pmc Functional antagonism between Helicobacter pylori CagA and vacuolating toxin VacA in control of the NFAT signaling pathway in gastric epithelial cells
    Kazuyuki Yokoyama
    Institute for Genetic Medicine, Hokkaido University, Sapporo 060 0815, Japan
    Proc Natl Acad Sci U S A 102:9661-6. 2005
    ..pylori infection...
  27. ncbi Concentrations of alpha- and beta-defensins in gastric juice of patients with various gastroduodenal diseases
    Yoshito Nishi
    Second Department of Internal Medicine, Nagasaki University School of Medicine, Japan
    World J Gastroenterol 11:99-103. 2005
    ..To determine the concentration of alpha- and beta-defensins in gastric juice of patients with various gastroduodenal diseases...
  28. ncbi Elevated concentrations of alpha-defensins in gastric juice of patients with Helicobacter pylori infection
    Hajime Isomoto
    Second Department of Internal Medicine, Nagasaki University School of Medicine, Nagasaki, Japan
    Am J Gastroenterol 99:1916-23. 2004
    ..Defensins (alpha- and beta-defensins) are endogenous antimicrobial peptides. Little is known about alpha-defensins during Helicobacter pylori infection...