Y Hiraoka

Summary

Affiliation: Communications Research Laboratory
Country: Japan

Publications

  1. ncbi request reprint ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe
    Akihisa Matsuyama
    Chemical Genetics Laboratory, RIKEN, Wako, Saitama 351 0198, Japan
    Nat Biotechnol 24:841-7. 2006
  2. pmc Overexpression of the human MNB/DYRK1A gene induces formation of multinucleate cells through overduplication of the centrosome
    Eishi Funakoshi
    Department of Biochemistry, Faculty of Pharmaceutical Sciences, Setsunan University, Osaka 573 0101, Japan
    BMC Cell Biol 4:12. 2003
  3. ncbi request reprint [Genome sequence of Schizosaccharomyces pombe]
    Yuji Chikashige
    Tanpakushitsu Kakusan Koso 47:1215-20. 2002
  4. ncbi request reprint Multispectral imaging fluorescence microscopy for living cells
    Yasushi Hiraoka
    Kansai Advanced Research Center, Communications Research Laboratory, Kobe, Japan
    Cell Struct Funct 27:367-74. 2002
  5. ncbi request reprint Meiotic telomeres: a matchmaker for homologous chromosomes
    Y Hiraoka
    Kansai Advanced Research Center, Communications Research Laboratory, Kobe, Japan
    Genes Cells 3:405-13. 1998
  6. ncbi request reprint Live fluorescence imaging reveals early recruitment of emerin, LBR, RanBP2, and Nup153 to reforming functional nuclear envelopes
    T Haraguchi
    Kansai Advanced Research Center, Communications Research Laboratory, CREST Research Project, Japan Science and Technology Corporation, Iwaoka cho, Nishi ku, Kobe 651 2492, Japan
    J Cell Sci 113:779-94. 2000
  7. ncbi request reprint A conserved protein, Nuf2, is implicated in connecting the centromere to the spindle during chromosome segregation: a link between the kinetochore function and the spindle checkpoint
    A Nabetani
    Cell Biology Group, Kansai Advanced Research Center, Communications Research Laboratory, Kobe, Japan
    Chromosoma 110:322-34. 2001
  8. ncbi request reprint Dynamics of chromosomes and microtubules visualized by multiple-wavelength fluorescence imaging in living mammalian cells: effects of mitotic inhibitors on cell cycle progression
    T Haraguchi
    Kansai Advanced Research Centre, Communications Research Laboratory, Kobe, Japan
    Genes Cells 2:369-80. 1997
  9. ncbi request reprint Characterization of fission yeast meiotic mutants based on live observation of meiotic prophase nuclear movement
    Y Hiraoka
    Kansai Advanced Research Center, Communications Research Laboratory, Kobe, Japan
    Chromosoma 109:103-9. 2000
  10. pmc Dynamic behavior of microtubules during dynein-dependent nuclear migrations of meiotic prophase in fission yeast
    A Yamamoto
    Cell Biology Group, Kansai Advanced Research Center, Communications Research Laboratory, Kobe 651 2492, Japan
    Mol Biol Cell 12:3933-46. 2001

Collaborators

Detail Information

Publications58

  1. ncbi request reprint ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe
    Akihisa Matsuyama
    Chemical Genetics Laboratory, RIKEN, Wako, Saitama 351 0198, Japan
    Nat Biotechnol 24:841-7. 2006
    ..Furthermore, using leptomycin B, an inhibitor of the nuclear export protein Crm1, we identified 285 proteins whose localization is regulated by Crm1...
  2. pmc Overexpression of the human MNB/DYRK1A gene induces formation of multinucleate cells through overduplication of the centrosome
    Eishi Funakoshi
    Department of Biochemistry, Faculty of Pharmaceutical Sciences, Setsunan University, Osaka 573 0101, Japan
    BMC Cell Biol 4:12. 2003
    ..This gene encodes a dual specificity protein kinase that catalyzes its autophosphorylation on serine/threonine and tyrosine residues. But, the functions of the MNB/DYRK1A gene in cellular processes are unknown...
  3. ncbi request reprint [Genome sequence of Schizosaccharomyces pombe]
    Yuji Chikashige
    Tanpakushitsu Kakusan Koso 47:1215-20. 2002
  4. ncbi request reprint Multispectral imaging fluorescence microscopy for living cells
    Yasushi Hiraoka
    Kansai Advanced Research Center, Communications Research Laboratory, Kobe, Japan
    Cell Struct Funct 27:367-74. 2002
    ..We introduce these systems with particular interest in live cell imaging. Finally we demonstrate examples of spectral imaging of living cells using commercially available systems with no need for user engineering...
  5. ncbi request reprint Meiotic telomeres: a matchmaker for homologous chromosomes
    Y Hiraoka
    Kansai Advanced Research Center, Communications Research Laboratory, Kobe, Japan
    Genes Cells 3:405-13. 1998
    ..Here I have summarized our current understanding of the meiotic behaviour of telomeres in S. pombe, and discuss the role of telomeres in meiosis...
  6. ncbi request reprint Live fluorescence imaging reveals early recruitment of emerin, LBR, RanBP2, and Nup153 to reforming functional nuclear envelopes
    T Haraguchi
    Kansai Advanced Research Center, Communications Research Laboratory, CREST Research Project, Japan Science and Technology Corporation, Iwaoka cho, Nishi ku, Kobe 651 2492, Japan
    J Cell Sci 113:779-94. 2000
    ..Taken together, these results showed that emerin, LBR, and several NPC components (RanBP2, Nup153, p62), but not Tpr, reconstitute around chromosomes very early in telophase prior to the recovery of nuclear import activity...
  7. ncbi request reprint A conserved protein, Nuf2, is implicated in connecting the centromere to the spindle during chromosome segregation: a link between the kinetochore function and the spindle checkpoint
    A Nabetani
    Cell Biology Group, Kansai Advanced Research Center, Communications Research Laboratory, Kobe, Japan
    Chromosoma 110:322-34. 2001
    ..We propose that Nuf2 acts at the centromere to establish a connection with the spindle for proper chromosome segregation, and that Nuf2 function is also required for the spindle checkpoint...
  8. ncbi request reprint Dynamics of chromosomes and microtubules visualized by multiple-wavelength fluorescence imaging in living mammalian cells: effects of mitotic inhibitors on cell cycle progression
    T Haraguchi
    Kansai Advanced Research Centre, Communications Research Laboratory, Kobe, Japan
    Genes Cells 2:369-80. 1997
    ..In order to be able to observe dynamic events in living mammalian cells, we have developed a computerized fluorescence microscope system...
  9. ncbi request reprint Characterization of fission yeast meiotic mutants based on live observation of meiotic prophase nuclear movement
    Y Hiraoka
    Kansai Advanced Research Center, Communications Research Laboratory, Kobe, Japan
    Chromosoma 109:103-9. 2000
    ....
  10. pmc Dynamic behavior of microtubules during dynein-dependent nuclear migrations of meiotic prophase in fission yeast
    A Yamamoto
    Cell Biology Group, Kansai Advanced Research Center, Communications Research Laboratory, Kobe 651 2492, Japan
    Mol Biol Cell 12:3933-46. 2001
    ....
  11. ncbi request reprint Telomere binding of the Rap1 protein is required for meiosis in fission yeast
    Y Chikashige
    CREST Research Project of the Japan Science and Technology Corporation, Kansai Advanced Research Center, Commmunications Research Laboratory, 588 2 Iwaoka, Iwaoka cho, Nishi ku, 651 2492, Kobe, Japan
    Curr Biol 11:1618-23. 2001
    ....
  12. ncbi request reprint BAF is required for emerin assembly into the reforming nuclear envelope
    T Haraguchi
    CREST Research Project of the Japan Science and Technology Corporation, Kansai Advanced Research Center, Communications Research Laboratory, 588 2 Iwaoka, Iwaoka cho, Nishi ku, Kobe 651 2492, Japan
    J Cell Sci 114:4575-85. 2001
    ..We conclude that BAF is required for the assembly of emerin and A-type lamins at the reforming NE during telophase, and may mediate their stability in the subsequent interphase...
  13. ncbi request reprint How do meiotic chromosomes meet their homologous partners?: lessons from fission yeast
    A Yamamoto
    Structural Biology Section and CREST Research Project, Kansai Advanced Research Center, Iwaoka, Nishi ku, Japan
    Bioessays 23:526-33. 2001
    ....
  14. pmc A cytoplasmic dynein heavy chain is required for oscillatory nuclear movement of meiotic prophase and efficient meiotic recombination in fission yeast
    A Yamamoto
    Kansai Advanced Research Center, Communications Research Laboratory, Kobe 651 2401, Japan
    J Cell Biol 145:1233-49. 1999
    ..These results strongly suggest that the dynein-driven nuclear movements of meiotic prophase are necessary for efficient pairing of homologous chromosomes in fission yeast, which in turn promotes efficient meiotic recombination...
  15. pmc A defect in protein farnesylation suppresses a loss of Schizosaccharomyces pombe tsc2+, a homolog of the human gene predisposing to tuberous sclerosis complex
    Yukiko Nakase
    Radiation Biology Center, Kyoto University, Japan
    Genetics 173:569-78. 2006
    ..On the basis of these results, we discuss TSC pathology and possible improvement in chemotherapy for TSC...
  16. ncbi request reprint Selective elimination of messenger RNA prevents an incidence of untimely meiosis
    Yuriko Harigaya
    Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113 0033, Japan
    Nature 442:45-50. 2006
    ..We propose that Mei2 turns off the DSR-Mmi1 system by sequestering Mmi1 to the dot and thereby secures stable expression of meiosis-specific transcripts...
  17. ncbi request reprint Meiotic proteins bqt1 and bqt2 tether telomeres to form the bouquet arrangement of chromosomes
    Yuji Chikashige
    Cell Biology Group and CREST Research Project, Kansai Advanced Research Center, National Institute of Information and Communications Technology, 588 2 Iwaoka, Iwaoka cho, Nishi ku, Kobe 651 2492, Japan
    Cell 125:59-69. 2006
    ..Significantly, when both Bqt1 and Bqt2 are ectopically expressed in mitotic cells, they also form a bridge between Rap1 and Sad1. Thus, a complex including Bqt1 and Bqt2 is essential for connecting telomeres to the SPB...
  18. ncbi request reprint The carboxy-terminus of Alp4 alters microtubule dynamics to induce oscillatory nuclear movement led by the spindle pole body in Schizosaccharomyces pombe
    Hirohisa Masuda
    Cell Biology Group and CREST JST, Kansai Advanced Research Center, National Institute of Information and Communications Technology, Kobe, 651 2492, Japan
    Genes Cells 11:337-52. 2006
    ....
  19. ncbi request reprint Modulation of Alp4 function in Schizosaccharomyces pombe induces novel phenotypes that imply distinct functions for nuclear and cytoplasmic gamma-tubulin complexes
    Hirohisa Masuda
    Cell Biology Group and CREST JST, Kansai Advanced Research Center, National Institute of Information and Communications Technology, Kobe, 651 2492, Japan
    Genes Cells 11:319-36. 2006
    ....
  20. ncbi request reprint Characterization of rec15, an early meiotic recombination gene in Schizosaccharomyces pombe
    Eveline Doll
    Institute of Cell Biology, University of Bern, Baltzerstrasse 4, CH 3012, Bern, Switzerland
    Curr Genet 48:323-33. 2005
    ..The early onset of meiotic events is a novel observation for an early recombination mutant and implies a function of rec15 protein already at or before DNA synthesis...
  21. ncbi request reprint [Dynamics of homologous chromosome pairing as observed in living cells of fission yeast]
    Da Qiao Ding
    Tanpakushitsu Kakusan Koso 50:131-5. 2005
  22. pmc The constitutive centromere component CENP-50 is required for recovery from spindle damage
    Yukinori Minoshima
    National Institute of Genetics, Mishima, Shizuoka 411 8540, Japan
    Mol Cell Biol 25:10315-28. 2005
    ..We also observed severe mitotic defects in CENP-50-deficient cells with apparent premature sister chromatid separation when the mitotic checkpoint was activated, indicating that CENP-50 is required for recovery from spindle damage...
  23. pmc Meiotic cohesins modulate chromosome compaction during meiotic prophase in fission yeast
    Da Qiao Ding
    Cell Biology Group, Kansai Advanced Research Center, National Institute of Information and Communications Technology, Kobe 651 2492, Japan
    J Cell Biol 174:499-508. 2006
    ....
  24. pmc Reconstruction of the kinetochore during meiosis in fission yeast Schizosaccharomyces pombe
    Aki Hayashi
    Kansai Advanced Research Center, National Institute of Information and Communications Technology, 588 2 Iwaoka, Kobe 651 2492, Japan
    Mol Biol Cell 17:5173-84. 2006
    ....
  25. doi request reprint Heterochromatin integrity affects chromosome reorganization after centromere dysfunction
    Kojiro Ishii
    Division of Cell Biology, Institute of Life Science, Kurume University, Japan
    Science 321:1088-91. 2008
    ..By affecting the modes of chromosomal reorganization, the genomic distribution of heterochromatin may influence the fate of karyotype evolution...
  26. pmc Spindle checkpoint activation at meiosis I advances anaphase II onset via meiosis-specific APC/C regulation
    Ayumu Yamamoto
    Department of Chemistry, Shizuoka University, Suruga ku, Shizuoka 422 8529, Japan
    J Cell Biol 182:277-88. 2008
    ..Our findings show that impacts of SAC activation are not confined to a single division at meiosis due to meiosis-specific APC/C regulation, which has probably been evolved for execution of two meiotic divisions...
  27. doi request reprint Live cell imaging and electron microscopy reveal dynamic processes of BAF-directed nuclear envelope assembly
    Tokuko Haraguchi
    CREST Research Project, Kobe Advanced ICT Research Center, National Institute of Information and Communications Technology, 588 2 Iwaoka, Iwaoka cho, Nishi ku, Kobe, Japan
    J Cell Sci 121:2540-54. 2008
    ..These results suggest that the MT-mediated BAF accumulation at the core facilitates NE assembly at the end of mitosis...
  28. ncbi request reprint Gene expression and distribution of Swi6 in partial aneuploids of the fission yeast Schizosaccharomyces pombe
    Yuji Chikashige
    Cell Biology Project, Biological ICT Group, Kobe Advanced ICT Research Center, National Institute of Information and Communications Technology, and CREST Research Project, Kobe, Japan
    Cell Struct Funct 32:149-61. 2007
    ..These results suggest that additional chromosomes in aneuploids could lead to imbalances in gene expression through changes in distribution of heterochromatin as well as in gene dosage...
  29. ncbi request reprint Two-step, extensive alterations in the transcriptome from G0 arrest to cell division in Schizosaccharomyces pombe
    Mizuki Shimanuki
    Initial Research Project IRP, Okinawa Institute of Science and Technology Promotion Corporation OIST, Uruma 904 2234, Okinawa, Japan
    Genes Cells 12:677-92. 2007
    ....
  30. ncbi request reprint Nuclear localization of barrier-to-autointegration factor is correlated with progression of S phase in human cells
    Tokuko Haraguchi
    CREST Research Project, Kansai Advanced Research Center, National Institute of Information and Communications Technology, 588 2 Iwaoka, Iwaoka cho, Nishi ku, Kobe 651 2492, Japan
    J Cell Sci 120:1967-77. 2007
    ..BAF downregulation also caused lamin A to mislocalize away from the nuclear envelope. These results indicate that BAF is required for the integrity of the nuclear lamina and normal progression of S phase in human cells...
  31. ncbi request reprint [Chromosome dynamics during meiosis]
    Haruhiko Asakawa
    Tanpakushitsu Kakusan Koso 51:2159-64. 2006
  32. ncbi request reprint [Fluorescence imaging to visualize molecular dynamics in living cells]
    Tokuko Haraguchi
    Tanpakushitsu Kakusan Koso 51:1972-7. 2006
  33. ncbi request reprint [Telomere bouquet of meiotic chromosomes]
    Yasushi Hiraoka
    Tanpakushitsu Kakusan Koso 52:145-50. 2007
  34. pmc Ect2 and MgcRacGAP regulate the activation and function of Cdc42 in mitosis
    Fabian Oceguera-Yanez
    Department of Pharmacology, Kyoto University Faculty of Medicine, Kyoto 606 8501, Japan
    J Cell Biol 168:221-32. 2005
    ..These results suggest that Ect2 and MgcRacGAP regulate the activation and function of Cdc42 in mitosis...
  35. ncbi request reprint Functional expression of human mitochondrial CYP11B2 in fission yeast and identification of a new internal electron transfer protein, etp1
    Matthias Bureik
    Universitat des Saarlandes, FR 8 8 Biochemie, Postfach 151150, D 66041 Saarbrucken, Germany
    Biochemistry 41:2311-21. 2002
    ..Therefore, we suggest to name this protein etp1 (electron-transfer protein 1)...
  36. ncbi request reprint Cell cycle behavior of human HP1 subtypes: distinct molecular domains of HP1 are required for their centromeric localization during interphase and metaphase
    Tomohiro Hayakawa
    CREST Research Project, Kansai Advanced Research Center, Communications Research Laboratory, 588 2 Iwaoka, Iwaoka cho, Nishi ku, Kobe 651 2492, Japan
    J Cell Sci 116:3327-38. 2003
    ....
  37. ncbi request reprint Dynamic behavior of Nuf2-Hec1 complex that localizes to the centrosome and centromere and is essential for mitotic progression in vertebrate cells
    Tetsuya Hori
    PRESTO, The Japan Science and Technology Corporation, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima, Shizuoka 411 8540, Japan
    J Cell Sci 116:3347-62. 2003
    ..Furthermore, photobleaching experiments revealed that the Nuf2-Hec1 complex is stably associated with the centromere and that interaction of this complex with the centrosome is dynamic...
  38. pmc Monopolar spindle attachment of sister chromatids is ensured by two distinct mechanisms at the first meiotic division in fission yeast
    Ayumu Yamamoto
    CREST Research Project, Kansai Advanced Research Center, Communications Research Laboratory, 588 2 Iwaoka, Iwaoka cho, Nishi ku, Kobe 651 2492, Japan
    EMBO J 22:2284-96. 2003
    ..We propose that co-operation of these two mechanisms generates the high fidelity of monopolar attachment...
  39. ncbi request reprint Linear element formation and their role in meiotic sister chromatid cohesion and chromosome pairing
    Monika Molnar
    CREST Research Project, Kansai Advanced Research Center, Communications Research Laboratory, Kobe 651 2492, Japan
    J Cell Sci 116:1719-31. 2003
    ..Investigation of the rec10 mutant showed that linear elements are basically dispensable for sister chromatid cohesion, but contribute to full level pairing of homologous chromosomes...
  40. ncbi request reprint Interaction of the chromatin compaction-inducing domain (LR domain) of Ki-67 antigen with HP1 proteins
    Ai Kametaka
    Department of Cell Biology and Neuroscience, Graduate School of Medicine, Osaka University, 2 2 Yamada oka, Suita, Japan
    Genes Cells 7:1231-42. 2002
    ..The possible human homologue of chmadrin, Ki-67 antigen (pKi-67), also has a stretch of LR pairs, but with no obvious overall similarity, at its C-terminus...
  41. ncbi request reprint Spectral imaging fluorescence microscopy
    Tokuko Haraguchi
    CREST Research Project, Kansai Advanced Research Center, Communications Research Laboratory, 588 2 Iwaoka, Iwaoka cho, Nishi ku, Kobe 651 2492, Japan
    Genes Cells 7:881-7. 2002
    ....
  42. ncbi request reprint CENP-I is essential for centromere function in vertebrate cells
    Ai Nishihashi
    PRESTO, The Japan Science and Technology Corporation, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima, 411 8540, Shizuoka, Japan
    Dev Cell 2:463-76. 2002
    ..Eventually, cells exited mitosis without undergoing cytokinesis. Immunocytochemical analysis of CENP-I-deficient cells demonstrated that both CENP-I and CENP-H are necessary for localization of CENP-C but not CENP-A to the centromere...
  43. pmc The 14-kDa dynein light chain-family protein Dlc1 is required for regular oscillatory nuclear movement and efficient recombination during meiotic prophase in fission yeast
    Futaba Miki
    Kazusa DNA Research Institute, Kisarazu 292 0812, Japan
    Mol Biol Cell 13:930-46. 2002
    ..S. pombe contains a homolog of the 8-kDa dynein light chain, Dlc2. This class of dynein light chain, however, is not essential in either the vegetative or sexual phases...
  44. ncbi request reprint Cytoplasmic dynein in fungi: insights from nuclear migration
    Ayumu Yamamoto
    CREST Research Project, Kansai Advanced Research Center, Communications Research Laboratory, 588 2 Iwaoka, Iwaoka cho, Nishi ku, Kobe 651 2492, Japan
    J Cell Sci 116:4501-12. 2003
    ..Recruitment of dynein to microtubules and its subsequent activation upon association with cellular structures are perhaps common to many dynein-dependent eukaryotic processes, including organelle transport...
  45. ncbi request reprint Emerin binding to Btf, a death-promoting transcriptional repressor, is disrupted by a missense mutation that causes Emery-Dreifuss muscular dystrophy
    Tokuko Haraguchi
    CREST Research Project, Kansai Advanced Research Center, Communications Research Laboratory, Iwaoka cho, Nishi ku, Kobe, Japan
    Eur J Biochem 271:1035-45. 2004
    ..These results suggest that Btf localization is regulated by apoptotic signals, and that loss of emerin binding to Btf may be relevant to muscle wasting in Emery-Dreifuss muscular dystrophy...
  46. ncbi request reprint Meiosis induced by inactivation of Pat1 kinase proceeds with aberrant nuclear positioning of centromeres in the fission yeast Schizosaccharomyces pombe
    Yuji Chikashige
    Cell Biology Group, Kansai Advanced Research Center, National Institute of Information and Communications Technology, 588 2 Iwaoka, Iwaoka cho, Nishi ku, Kobe 651 2492, Japan
    Genes Cells 9:671-84. 2004
    ..As Pat1 inactivation is often useful to induce S. pombe cells synchronously into meiosis, the temporal order of chromosomal events determined here will provide landmarks for the progression of meiosis downstream the Pat1 inactivation...
  47. ncbi request reprint Activation of the pheromone-responsive MAP kinase drives haploid cells to undergo ectopic meiosis with normal telomere clustering and sister chromatid segregation in fission yeast
    Takaharu G Yamamoto
    Cell Biology Group and CREST Research Project, Kansai Advanced Research Center, National Institute of Information and Communication Technology, 588 2 Iwaoka cho, Iwaoka, Nishi ku, Kobe 651 2492, Japan
    J Cell Sci 117:3875-86. 2004
    ..In contrast, in meiosis induced by inactivation of Pat1, sister chromatids segregate precociously during the first meiotic division. Thus, these results suggest that activation of Spk1 drives meiosis in S. pombe...
  48. pmc Dissociation of the Nuf2-Ndc80 complex releases centromeres from the spindle-pole body during meiotic prophase in fission yeast
    Haruhiko Asakawa
    Cell Biology Group and CREST Research Project, Kansai Advanced Research Center, National Institute of Information and Communications Technology, Kobe 651 2492, Japan
    Mol Biol Cell 16:2325-38. 2005
    ....
  49. ncbi request reprint Dynamics of homologous chromosome pairing during meiotic prophase in fission yeast
    Da Qiao Ding
    CREST Research Project, Kansai Advanced Research Center, 588 2 Iwaoka, Iwaoka cho, Nishi ku, Kobe 651 2492, Japan
    Dev Cell 6:329-41. 2004
    ....
  50. ncbi request reprint Dynamic interaction between BAF and emerin revealed by FRAP, FLIP, and FRET analyses in living HeLa cells
    Takeshi Shimi
    CREST of JST, Kansai Advanced Research Center, CRL, Kobe 651 2492, Japan
    J Struct Biol 147:31-41. 2004
    ..These findings contrast with the slow mobility of both GFP-BAF and GFP-emerin during telophase, when they colocalized at the 'core' region of telophase chromosomes at early stages of nuclear assembly...
  51. ncbi request reprint Heat-shock induced nuclear retention and recycling inhibition of importin alpha
    Maiko Furuta
    Cellular Dynamics Laboratory, Discovery Research Institute, RIKEN, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Genes Cells 9:429-41. 2004
    ..This suggests a new regulatory mechanism for the adaptation of cells to environmental changes, such as heat-shock...
  52. pmc Peroxisomes are formed from complex membrane structures in PEX6-deficient CHO cells upon genetic complementation
    Noriyo Hashiguchi
    Department of Life Science, Faculty of Science, Himeji Institute of Technology, Kamigori, Hyogo 678 1297, Japan
    Mol Biol Cell 13:711-22. 2002
    ..Our results indicate that 1) Peroxisomal ghosts are complex membrane structures; and 2) The complex membrane structures become import competent and are converted into peroxisomes upon complementation with PEX6...
  53. pmc Cellular stresses induce the nuclear accumulation of importin alpha and cause a conventional nuclear import block
    Yoichi Miyamoto
    Department of Frontier Biosciences, Graduate School of Frontier Biosciences, Osaka University, 1 3 Yamada oka, Suita, Osaka 565 0871, Japan
    J Cell Biol 165:617-23. 2004
    ..These findings suggest that the stress-induced nuclear accumulation of importin alpha can be involved in a common physiological response to various stress conditions...
  54. ncbi request reprint Another way to move chromosomes
    Yuji Chikashige
    Kobe Advanced ICT Research Center, National Institute of Information and Communications Technology, 588 2 Iwaoka, Iwaoka cho, Nishi ku, Kobe, 651 2492, Japan
    Chromosoma 116:497-505. 2007
    ..Telomeres are then moved along the nuclear envelope using cytoplasmic microtubules. These findings illustrate a general mechanism for transmitting a cytoskeletal driving force to chromosomes across the nuclear envelope...
  55. ncbi request reprint Functional domain analysis of human HP1 isoforms in Drosophila
    Masaki Kato
    Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo ku, Kyoto, Japan
    Cell Struct Funct 32:57-67. 2007
    ..These results suggest human HP1 subtypes use different domains to suppress gene expression in Drosophila cells...
  56. ncbi request reprint Nuclear structure-associated TIF2 recruits glucocorticoid receptor and its target DNA
    Hidesato Ogawa
    Department of Biochemistry, Institute for Virus Research, Kyoto University, Japan
    Biochem Biophys Res Commun 320:218-25. 2004
    ..The TIF2 foci could recruit GR carrying a microinjected GR responsive element. We propose that TIF2 provides a nuclear compartment that allows the assembly of multi-protein complexes required for GR-mediated gene activation...
  57. ncbi request reprint Mutations in dynein link motor neuron degeneration to defects in retrograde transport
    Majid Hafezparast
    Department of Neurodegenerative Disease, Institute of Neurology, National Hospital for Neurology and Neurosurgery, Queen Square, London WC1N 3BG, UK
    Science 300:808-12. 2003
    ..These mutations exclusively perturb neuron-specific functions of dynein...
  58. pmc Cadherin activity is required for activity-induced spine remodeling
    Ko Okamura
    Department of Pharmacology, Osaka University Medical School, Suita, Japan
    J Cell Biol 167:961-72. 2004
    ..Together, our data suggest that cadherin-based adhesion machinery coupled with the actin-cytoskeleton is critical for the remodeling of synaptic apposition zone...