Tokuko Haraguchi

Summary

Affiliation: Communications Research Laboratory
Country: Japan

Publications

  1. pmc Overexpression of the human MNB/DYRK1A gene induces formation of multinucleate cells through overduplication of the centrosome
    Eishi Funakoshi
    Department of Biochemistry, Faculty of Pharmaceutical Sciences, Setsunan University, Osaka 573 0101, Japan
    BMC Cell Biol 4:12. 2003
  2. ncbi Live cell imaging: approaches for studying protein dynamics in living cells
    Tokuko Haraguchi
    CREST Research Project of the Japan Science and Technology Corporation, Kansai Advanced Research Center, Kobe, Japan
    Cell Struct Funct 27:333-4. 2002
  3. ncbi Dynamics of chromosomes and microtubules visualized by multiple-wavelength fluorescence imaging in living mammalian cells: effects of mitotic inhibitors on cell cycle progression
    T Haraguchi
    Kansai Advanced Research Centre, Communications Research Laboratory, Kobe, Japan
    Genes Cells 2:369-80. 1997
  4. ncbi BAF is required for emerin assembly into the reforming nuclear envelope
    T Haraguchi
    CREST Research Project of the Japan Science and Technology Corporation, Kansai Advanced Research Center, Communications Research Laboratory, 588 2 Iwaoka, Iwaoka cho, Nishi ku, Kobe 651 2492, Japan
    J Cell Sci 114:4575-85. 2001
  5. ncbi Live fluorescence imaging reveals early recruitment of emerin, LBR, RanBP2, and Nup153 to reforming functional nuclear envelopes
    T Haraguchi
    Kansai Advanced Research Center, Communications Research Laboratory, CREST Research Project, Japan Science and Technology Corporation, Iwaoka cho, Nishi ku, Kobe 651 2492, Japan
    J Cell Sci 113:779-94. 2000
  6. ncbi Emerin binding to Btf, a death-promoting transcriptional repressor, is disrupted by a missense mutation that causes Emery-Dreifuss muscular dystrophy
    Tokuko Haraguchi
    CREST Research Project, Kansai Advanced Research Center, Communications Research Laboratory, Iwaoka cho, Nishi ku, Kobe, Japan
    Eur J Biochem 271:1035-45. 2004
  7. doi Live cell imaging and electron microscopy reveal dynamic processes of BAF-directed nuclear envelope assembly
    Tokuko Haraguchi
    CREST Research Project, Kobe Advanced ICT Research Center, National Institute of Information and Communications Technology, 588 2 Iwaoka, Iwaoka cho, Nishi ku, Kobe, Japan
    J Cell Sci 121:2540-54. 2008
  8. ncbi Dynamic interaction between BAF and emerin revealed by FRAP, FLIP, and FRET analyses in living HeLa cells
    Takeshi Shimi
    CREST of JST, Kansai Advanced Research Center, CRL, Kobe 651 2492, Japan
    J Struct Biol 147:31-41. 2004
  9. pmc Reconstruction of the kinetochore during meiosis in fission yeast Schizosaccharomyces pombe
    Aki Hayashi
    Kansai Advanced Research Center, National Institute of Information and Communications Technology, 588 2 Iwaoka, Kobe 651 2492, Japan
    Mol Biol Cell 17:5173-84. 2006
  10. ncbi The carboxy-terminus of Alp4 alters microtubule dynamics to induce oscillatory nuclear movement led by the spindle pole body in Schizosaccharomyces pombe
    Hirohisa Masuda
    Cell Biology Group and CREST JST, Kansai Advanced Research Center, National Institute of Information and Communications Technology, Kobe, 651 2492, Japan
    Genes Cells 11:337-52. 2006

Collaborators

Detail Information

Publications46

  1. pmc Overexpression of the human MNB/DYRK1A gene induces formation of multinucleate cells through overduplication of the centrosome
    Eishi Funakoshi
    Department of Biochemistry, Faculty of Pharmaceutical Sciences, Setsunan University, Osaka 573 0101, Japan
    BMC Cell Biol 4:12. 2003
    ..This gene encodes a dual specificity protein kinase that catalyzes its autophosphorylation on serine/threonine and tyrosine residues. But, the functions of the MNB/DYRK1A gene in cellular processes are unknown...
  2. ncbi Live cell imaging: approaches for studying protein dynamics in living cells
    Tokuko Haraguchi
    CREST Research Project of the Japan Science and Technology Corporation, Kansai Advanced Research Center, Kobe, Japan
    Cell Struct Funct 27:333-4. 2002
    ..In this special issue of review articles, we review various imaging technologies of microscope hardware and fluorescent probes useful for cell biologists, with a focus on recent development of live cell imaging...
  3. ncbi Dynamics of chromosomes and microtubules visualized by multiple-wavelength fluorescence imaging in living mammalian cells: effects of mitotic inhibitors on cell cycle progression
    T Haraguchi
    Kansai Advanced Research Centre, Communications Research Laboratory, Kobe, Japan
    Genes Cells 2:369-80. 1997
    ..In order to be able to observe dynamic events in living mammalian cells, we have developed a computerized fluorescence microscope system...
  4. ncbi BAF is required for emerin assembly into the reforming nuclear envelope
    T Haraguchi
    CREST Research Project of the Japan Science and Technology Corporation, Kansai Advanced Research Center, Communications Research Laboratory, 588 2 Iwaoka, Iwaoka cho, Nishi ku, Kobe 651 2492, Japan
    J Cell Sci 114:4575-85. 2001
    ..We conclude that BAF is required for the assembly of emerin and A-type lamins at the reforming NE during telophase, and may mediate their stability in the subsequent interphase...
  5. ncbi Live fluorescence imaging reveals early recruitment of emerin, LBR, RanBP2, and Nup153 to reforming functional nuclear envelopes
    T Haraguchi
    Kansai Advanced Research Center, Communications Research Laboratory, CREST Research Project, Japan Science and Technology Corporation, Iwaoka cho, Nishi ku, Kobe 651 2492, Japan
    J Cell Sci 113:779-94. 2000
    ..Taken together, these results showed that emerin, LBR, and several NPC components (RanBP2, Nup153, p62), but not Tpr, reconstitute around chromosomes very early in telophase prior to the recovery of nuclear import activity...
  6. ncbi Emerin binding to Btf, a death-promoting transcriptional repressor, is disrupted by a missense mutation that causes Emery-Dreifuss muscular dystrophy
    Tokuko Haraguchi
    CREST Research Project, Kansai Advanced Research Center, Communications Research Laboratory, Iwaoka cho, Nishi ku, Kobe, Japan
    Eur J Biochem 271:1035-45. 2004
    ..These results suggest that Btf localization is regulated by apoptotic signals, and that loss of emerin binding to Btf may be relevant to muscle wasting in Emery-Dreifuss muscular dystrophy...
  7. doi Live cell imaging and electron microscopy reveal dynamic processes of BAF-directed nuclear envelope assembly
    Tokuko Haraguchi
    CREST Research Project, Kobe Advanced ICT Research Center, National Institute of Information and Communications Technology, 588 2 Iwaoka, Iwaoka cho, Nishi ku, Kobe, Japan
    J Cell Sci 121:2540-54. 2008
    ..These results suggest that the MT-mediated BAF accumulation at the core facilitates NE assembly at the end of mitosis...
  8. ncbi Dynamic interaction between BAF and emerin revealed by FRAP, FLIP, and FRET analyses in living HeLa cells
    Takeshi Shimi
    CREST of JST, Kansai Advanced Research Center, CRL, Kobe 651 2492, Japan
    J Struct Biol 147:31-41. 2004
    ..These findings contrast with the slow mobility of both GFP-BAF and GFP-emerin during telophase, when they colocalized at the 'core' region of telophase chromosomes at early stages of nuclear assembly...
  9. pmc Reconstruction of the kinetochore during meiosis in fission yeast Schizosaccharomyces pombe
    Aki Hayashi
    Kansai Advanced Research Center, National Institute of Information and Communications Technology, 588 2 Iwaoka, Kobe 651 2492, Japan
    Mol Biol Cell 17:5173-84. 2006
    ....
  10. ncbi The carboxy-terminus of Alp4 alters microtubule dynamics to induce oscillatory nuclear movement led by the spindle pole body in Schizosaccharomyces pombe
    Hirohisa Masuda
    Cell Biology Group and CREST JST, Kansai Advanced Research Center, National Institute of Information and Communications Technology, Kobe, 651 2492, Japan
    Genes Cells 11:337-52. 2006
    ....
  11. pmc Membrane proteins Bqt3 and -4 anchor telomeres to the nuclear envelope to ensure chromosomal bouquet formation
    Yuji Chikashige
    Kobe Advanced ICT Research Center, National Institute of Information and Communications Technology, Nishi ku, Kobe 651 2492, Japan
    J Cell Biol 187:413-27. 2009
    ..Interestingly, the functional integrity of telomeres is maintained even when they are separated from the nuclear envelope in vegetative cells...
  12. pmc Monoclonal antibodies recognize gly-leu-phe-gly repeat of nucleoporin nup98 of tetrahymena, yeasts, and humans
    Masaaki Iwamoto
    Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology NICT, Kobe, Japan
    Monoclon Antib Immunodiagn Immunother 32:81-90. 2013
    ..Thus, these MAbs are useful in studying a wide variety of biological phenomena that involve Nup98, ranging from ciliate nuclear dimorphism to NUP98-related human leukemia...
  13. ncbi Meiotic proteins bqt1 and bqt2 tether telomeres to form the bouquet arrangement of chromosomes
    Yuji Chikashige
    Cell Biology Group and CREST Research Project, Kansai Advanced Research Center, National Institute of Information and Communications Technology, 588 2 Iwaoka, Iwaoka cho, Nishi ku, Kobe 651 2492, Japan
    Cell 125:59-69. 2006
    ..Significantly, when both Bqt1 and Bqt2 are ectopically expressed in mitotic cells, they also form a bridge between Rap1 and Sad1. Thus, a complex including Bqt1 and Bqt2 is essential for connecting telomeres to the SPB...
  14. doi Meiosis-specific noncoding RNA mediates robust pairing of homologous chromosomes in meiosis
    Da Qiao Ding
    Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology, Kobe 651 2492, Japan
    Science 336:732-6. 2012
    ..Thus, we propose that RNA transcripts retained on the chromosome play an active role in recognition of homologous chromosomes for pairing...
  15. ncbi Nuclear localization of barrier-to-autointegration factor is correlated with progression of S phase in human cells
    Tokuko Haraguchi
    CREST Research Project, Kansai Advanced Research Center, National Institute of Information and Communications Technology, 588 2 Iwaoka, Iwaoka cho, Nishi ku, Kobe 651 2492, Japan
    J Cell Sci 120:1967-77. 2007
    ..BAF downregulation also caused lamin A to mislocalize away from the nuclear envelope. These results indicate that BAF is required for the integrity of the nuclear lamina and normal progression of S phase in human cells...
  16. pmc Meiotic cohesins modulate chromosome compaction during meiotic prophase in fission yeast
    Da Qiao Ding
    Cell Biology Group, Kansai Advanced Research Center, National Institute of Information and Communications Technology, Kobe 651 2492, Japan
    J Cell Biol 174:499-508. 2006
    ....
  17. ncbi Gene expression and distribution of Swi6 in partial aneuploids of the fission yeast Schizosaccharomyces pombe
    Yuji Chikashige
    Cell Biology Project, Biological ICT Group, Kobe Advanced ICT Research Center, National Institute of Information and Communications Technology, and CREST Research Project, Kobe, Japan
    Cell Struct Funct 32:149-61. 2007
    ..These results suggest that additional chromosomes in aneuploids could lead to imbalances in gene expression through changes in distribution of heterochromatin as well as in gene dosage...
  18. ncbi Meiosis induced by inactivation of Pat1 kinase proceeds with aberrant nuclear positioning of centromeres in the fission yeast Schizosaccharomyces pombe
    Yuji Chikashige
    Cell Biology Group, Kansai Advanced Research Center, National Institute of Information and Communications Technology, 588 2 Iwaoka, Iwaoka cho, Nishi ku, Kobe 651 2492, Japan
    Genes Cells 9:671-84. 2004
    ..As Pat1 inactivation is often useful to induce S. pombe cells synchronously into meiosis, the temporal order of chromosomal events determined here will provide landmarks for the progression of meiosis downstream the Pat1 inactivation...
  19. ncbi Modulation of Alp4 function in Schizosaccharomyces pombe induces novel phenotypes that imply distinct functions for nuclear and cytoplasmic gamma-tubulin complexes
    Hirohisa Masuda
    Cell Biology Group and CREST JST, Kansai Advanced Research Center, National Institute of Information and Communications Technology, Kobe, 651 2492, Japan
    Genes Cells 11:319-36. 2006
    ....
  20. pmc Dissociation of the Nuf2-Ndc80 complex releases centromeres from the spindle-pole body during meiotic prophase in fission yeast
    Haruhiko Asakawa
    Cell Biology Group and CREST Research Project, Kansai Advanced Research Center, National Institute of Information and Communications Technology, Kobe 651 2492, Japan
    Mol Biol Cell 16:2325-38. 2005
    ....
  21. ncbi Spectral imaging fluorescence microscopy
    Tokuko Haraguchi
    CREST Research Project, Kansai Advanced Research Center, Communications Research Laboratory, 588 2 Iwaoka, Iwaoka cho, Nishi ku, Kobe 651 2492, Japan
    Genes Cells 7:881-7. 2002
    ....
  22. ncbi Multispectral imaging fluorescence microscopy for living cells
    Yasushi Hiraoka
    Kansai Advanced Research Center, Communications Research Laboratory, Kobe, Japan
    Cell Struct Funct 27:367-74. 2002
    ..We introduce these systems with particular interest in live cell imaging. Finally we demonstrate examples of spectral imaging of living cells using commercially available systems with no need for user engineering...
  23. ncbi Artificial induction of autophagy around polystyrene beads in nonphagocytic cells
    Shouhei Kobayashi
    Kobe Advanced ICT Research Center, National Institute of Information and Communications Technology, Iwaoka cho, Nishi ku, Kobe, Japan
    Autophagy 6:36-45. 2010
    ..In addition, the beads were subsequently transferred to lysosomes within a couple of hours. Our findings demonstrate the cellular responses that lead to autophagy in response to pathogen invasion...
  24. pmc Chromosomally-retained RNA mediates homologous pairing
    Da Qiao Ding
    Advanced ICT Research Institute Kobe, National Institute of Information and Communication Technology, Kobe, Japan
    Nucleus 3:516-9. 2012
    ..Acting as fiducial markers of homologous loci they would abrogate the need for direct DNA sequence homology searching...
  25. pmc The role of chromosomal retention of noncoding RNA in meiosis
    Da Qiao Ding
    Advanced ICT Research Institute Kobe, National Institute of Information and Communication Technology, Kobe, 651 2492, Japan
    Chromosome Res 21:665-72. 2013
    ..Although different organisms may have developed other or additional systems that are involved in chromosome pairing, the findings in S. pombe will provide new insights into understanding the roles of noncoding RNA in meiosis...
  26. doi Early entry and deformation of macropinosomes correlates with high efficiency of decaarginine-polyethylene glycol-lipid-mediated gene delivery
    Shouhei Kobayashi
    Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology, Iwaoka, Kobe, Japan
    J Gene Med 14:262-71. 2012
    ..However, the intracellular behaviour of the two different nanostructures, which leads to differences in gene delivery, remains to be elucidated...
  27. doi From meiosis to postmeiotic events: alignment and recognition of homologous chromosomes in meiosis
    Da Qiao Ding
    Kobe Advanced ICT Research Center, National Institute of Information and Communications Technology, Kobe, Japan
    FEBS J 277:565-70. 2010
    ..Here, we discuss the various mechanisms used for alignment and recognition of homologous chromosomes in meiosis...
  28. doi Two distinct repeat sequences of Nup98 nucleoporins characterize dual nuclei in the binucleated ciliate tetrahymena
    Masaaki Iwamoto
    Kobe Advanced ICT Research Center, National Institute of Information and Communications Technology, Kobe 651 2492, Japan
    Curr Biol 19:843-7. 2009
    ..These results suggest that Nup98s act as a barrier to misdirected localization of nucleus-specific proteins. Our findings provide the first evidence that the NPC contributes to nucleus-selective transport in ciliates...
  29. ncbi Cell cycle behavior of human HP1 subtypes: distinct molecular domains of HP1 are required for their centromeric localization during interphase and metaphase
    Tomohiro Hayakawa
    CREST Research Project, Kansai Advanced Research Center, Communications Research Laboratory, 588 2 Iwaoka, Iwaoka cho, Nishi ku, Kobe 651 2492, Japan
    J Cell Sci 116:3327-38. 2003
    ....
  30. ncbi Dynamics of homologous chromosome pairing during meiotic prophase in fission yeast
    Da Qiao Ding
    CREST Research Project, Kansai Advanced Research Center, 588 2 Iwaoka, Iwaoka cho, Nishi ku, Kobe 651 2492, Japan
    Dev Cell 6:329-41. 2004
    ....
  31. ncbi Another way to move chromosomes
    Yuji Chikashige
    Kobe Advanced ICT Research Center, National Institute of Information and Communications Technology, 588 2 Iwaoka, Iwaoka cho, Nishi ku, Kobe, 651 2492, Japan
    Chromosoma 116:497-505. 2007
    ..Telomeres are then moved along the nuclear envelope using cytoplasmic microtubules. These findings illustrate a general mechanism for transmitting a cytoskeletal driving force to chromosomes across the nuclear envelope...
  32. doi Puromycin resistance gene as an effective selection marker for ciliate Tetrahymena
    Masaaki Iwamoto
    Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology NICT, Kobe 651 2492, Japan
    Gene 534:249-55. 2014
    ..These results indicate that pac can be used as a selection marker in molecular studies of T. thermophila...
  33. pmc Ect2 and MgcRacGAP regulate the activation and function of Cdc42 in mitosis
    Fabian Oceguera-Yanez
    Department of Pharmacology, Kyoto University Faculty of Medicine, Kyoto 606 8501, Japan
    J Cell Biol 168:221-32. 2005
    ..These results suggest that Ect2 and MgcRacGAP regulate the activation and function of Cdc42 in mitosis...
  34. ncbi Dynamic behavior of Nuf2-Hec1 complex that localizes to the centrosome and centromere and is essential for mitotic progression in vertebrate cells
    Tetsuya Hori
    PRESTO, The Japan Science and Technology Corporation, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima, Shizuoka 411 8540, Japan
    J Cell Sci 116:3347-62. 2003
    ..Furthermore, photobleaching experiments revealed that the Nuf2-Hec1 complex is stably associated with the centromere and that interaction of this complex with the centrosome is dynamic...
  35. pmc Emerin is hyperphosphorylated and redistributed in herpes simplex virus type 1-infected cells in a manner dependent on both UL34 and US3
    Natalie Leach
    Department of Microbiology, The University of Iowa, 3 432 Bowen Science Building, Iowa City, IA 52242, USA
    J Virol 81:10792-803. 2007
    ....
  36. ncbi Interaction of the chromatin compaction-inducing domain (LR domain) of Ki-67 antigen with HP1 proteins
    Ai Kametaka
    Department of Cell Biology and Neuroscience, Graduate School of Medicine, Osaka University, 2 2 Yamada oka, Suita, Japan
    Genes Cells 7:1231-42. 2002
    ..The possible human homologue of chmadrin, Ki-67 antigen (pKi-67), also has a stretch of LR pairs, but with no obvious overall similarity, at its C-terminus...
  37. ncbi LAP2alpha and BAF transiently localize to telomeres and specific regions on chromatin during nuclear assembly
    Thomas Dechat
    Max F Perutz Laboratories, University Departments at the Vienna Biocenter, Department of Medical Biochemistry, Medical University of Vienna, 1030 Vienna, Austria
    J Cell Sci 117:6117-28. 2004
    ..We propose that LAP2alpha and a subfraction of BAF form defined complexes in chromatin core regions and may be involved in chromatin reorganization during early stages of nuclear assembly...
  38. ncbi [Fluorescence imaging to visualize molecular dynamics in living cells]
    Tokuko Haraguchi
    Tanpakushitsu Kakusan Koso 51:1972-7. 2006
  39. pmc Peroxisomes are formed from complex membrane structures in PEX6-deficient CHO cells upon genetic complementation
    Noriyo Hashiguchi
    Department of Life Science, Faculty of Science, Himeji Institute of Technology, Kamigori, Hyogo 678 1297, Japan
    Mol Biol Cell 13:711-22. 2002
    ..Our results indicate that 1) Peroxisomal ghosts are complex membrane structures; and 2) The complex membrane structures become import competent and are converted into peroxisomes upon complementation with PEX6...
  40. ncbi Heat-shock induced nuclear retention and recycling inhibition of importin alpha
    Maiko Furuta
    Cellular Dynamics Laboratory, Discovery Research Institute, RIKEN, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Genes Cells 9:429-41. 2004
    ..This suggests a new regulatory mechanism for the adaptation of cells to environmental changes, such as heat-shock...
  41. pmc Cellular stresses induce the nuclear accumulation of importin alpha and cause a conventional nuclear import block
    Yoichi Miyamoto
    Department of Frontier Biosciences, Graduate School of Frontier Biosciences, Osaka University, 1 3 Yamada oka, Suita, Osaka 565 0871, Japan
    J Cell Biol 165:617-23. 2004
    ..These findings suggest that the stress-induced nuclear accumulation of importin alpha can be involved in a common physiological response to various stress conditions...
  42. ncbi Nuclear structure-associated TIF2 recruits glucocorticoid receptor and its target DNA
    Hidesato Ogawa
    Department of Biochemistry, Institute for Virus Research, Kyoto University, Japan
    Biochem Biophys Res Commun 320:218-25. 2004
    ..The TIF2 foci could recruit GR carrying a microinjected GR responsive element. We propose that TIF2 provides a nuclear compartment that allows the assembly of multi-protein complexes required for GR-mediated gene activation...
  43. ncbi Functional domain analysis of human HP1 isoforms in Drosophila
    Masaki Kato
    Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo ku, Kyoto, Japan
    Cell Struct Funct 32:57-67. 2007
    ..These results suggest human HP1 subtypes use different domains to suppress gene expression in Drosophila cells...
  44. ncbi [Dynamics of the nuclear envelope in the cell cycle]
    Shouhei Kobayashi
    Tanpakushitsu Kakusan Koso 51:2165-71. 2006
  45. pmc The constitutive centromere component CENP-50 is required for recovery from spindle damage
    Yukinori Minoshima
    National Institute of Genetics, Mishima, Shizuoka 411 8540, Japan
    Mol Cell Biol 25:10315-28. 2005
    ..We also observed severe mitotic defects in CENP-50-deficient cells with apparent premature sister chromatid separation when the mitotic checkpoint was activated, indicating that CENP-50 is required for recovery from spindle damage...
  46. pmc The assembly and maintenance of heterochromatin initiated by transgene repeats are independent of the RNA interference pathway in mammalian cells
    Fangwei Wang
    Genomics Research Institute, Utsunomiya University, 350 Mine machi, Utsunomiya, Tochigi 321 8505, Japan
    Mol Cell Biol 26:4028-40. 2006
    ..Our results suggest that the RNAi pathway is not required for the assembly and maintenance of noncentromeric heterochromatin initiated by tandem transgene repeats in mammalian cells...