Research Topics
Genomes and Genes | Tokuko HaraguchiSummaryAffiliation: Communications Research Laboratory Country: Japan Publications
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Publications
Overexpression of the human MNB/DYRK1A gene induces formation of multinucleate cells through overduplication of the centrosomeEishi Funakoshi
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Setsunan University, Osaka 573 0101, Japan
BMC Cell Biol 4:12. 2003..This gene encodes a dual specificity protein kinase that catalyzes its autophosphorylation on serine/threonine and tyrosine residues. But, the functions of the MNB/DYRK1A gene in cellular processes are unknown...
Live cell imaging: approaches for studying protein dynamics in living cellsTokuko Haraguchi
CREST Research Project of the Japan Science and Technology Corporation, Kansai Advanced Research Center, Kobe, Japan
Cell Struct Funct 27:333-4. 2002..In this special issue of review articles, we review various imaging technologies of microscope hardware and fluorescent probes useful for cell biologists, with a focus on recent development of live cell imaging...- Dynamics of chromosomes and microtubules visualized by multiple-wavelength fluorescence imaging in living mammalian cells: effects of mitotic inhibitors on cell cycle progressionT Haraguchi
Kansai Advanced Research Centre, Communications Research Laboratory, Kobe, Japan
Genes Cells 2:369-80. 1997..In order to be able to observe dynamic events in living mammalian cells, we have developed a computerized fluorescence microscope system... - BAF is required for emerin assembly into the reforming nuclear envelopeT Haraguchi
CREST Research Project of the Japan Science and Technology Corporation, Kansai Advanced Research Center, Communications Research Laboratory, 588 2 Iwaoka, Iwaoka cho, Nishi ku, Kobe 651 2492, Japan
J Cell Sci 114:4575-85. 2001..We conclude that BAF is required for the assembly of emerin and A-type lamins at the reforming NE during telophase, and may mediate their stability in the subsequent interphase... - Live fluorescence imaging reveals early recruitment of emerin, LBR, RanBP2, and Nup153 to reforming functional nuclear envelopesT Haraguchi
Kansai Advanced Research Center, Communications Research Laboratory, CREST Research Project, Japan Science and Technology Corporation, Iwaoka cho, Nishi ku, Kobe 651 2492, Japan
J Cell Sci 113:779-94. 2000..Taken together, these results showed that emerin, LBR, and several NPC components (RanBP2, Nup153, p62), but not Tpr, reconstitute around chromosomes very early in telophase prior to the recovery of nuclear import activity... - Emerin binding to Btf, a death-promoting transcriptional repressor, is disrupted by a missense mutation that causes Emery-Dreifuss muscular dystrophyTokuko Haraguchi
CREST Research Project, Kansai Advanced Research Center, Communications Research Laboratory, Iwaoka cho, Nishi ku, Kobe, Japan
Eur J Biochem 271:1035-45. 2004..These results suggest that Btf localization is regulated by apoptotic signals, and that loss of emerin binding to Btf may be relevant to muscle wasting in Emery-Dreifuss muscular dystrophy... 
Live cell imaging and electron microscopy reveal dynamic processes of BAF-directed nuclear envelope assemblyTokuko Haraguchi
CREST Research Project, Kobe Advanced ICT Research Center, National Institute of Information and Communications Technology, 588 2 Iwaoka, Iwaoka cho, Nishi ku, Kobe, Japan
J Cell Sci 121:2540-54. 2008..These results suggest that the MT-mediated BAF accumulation at the core facilitates NE assembly at the end of mitosis...- Dynamic interaction between BAF and emerin revealed by FRAP, FLIP, and FRET analyses in living HeLa cellsTakeshi Shimi
CREST of JST, Kansai Advanced Research Center, CRL, Kobe 651 2492, Japan
J Struct Biol 147:31-41. 2004..These findings contrast with the slow mobility of both GFP-BAF and GFP-emerin during telophase, when they colocalized at the 'core' region of telophase chromosomes at early stages of nuclear assembly... - The carboxy-terminus of Alp4 alters microtubule dynamics to induce oscillatory nuclear movement led by the spindle pole body in Schizosaccharomyces pombeHirohisa Masuda
Cell Biology Group and CREST JST, Kansai Advanced Research Center, National Institute of Information and Communications Technology, Kobe, 651 2492, Japan
Genes Cells 11:337-52. 2006.... - Reconstruction of the kinetochore during meiosis in fission yeast Schizosaccharomyces pombeAki Hayashi
Kansai Advanced Research Center, National Institute of Information and Communications Technology, 588 2 Iwaoka, Kobe 651 2492, Japan
Mol Biol Cell 17:5173-84. 2006.... - Membrane proteins Bqt3 and -4 anchor telomeres to the nuclear envelope to ensure chromosomal bouquet formationYuji Chikashige
Kobe Advanced ICT Research Center, National Institute of Information and Communications Technology, Nishi ku, Kobe 651 2492, Japan
J Cell Biol 187:413-27. 2009..Interestingly, the functional integrity of telomeres is maintained even when they are separated from the nuclear envelope in vegetative cells... - Meiotic proteins bqt1 and bqt2 tether telomeres to form the bouquet arrangement of chromosomesYuji Chikashige
Cell Biology Group and CREST Research Project, Kansai Advanced Research Center, National Institute of Information and Communications Technology, 588 2 Iwaoka, Iwaoka cho, Nishi ku, Kobe 651 2492, Japan
Cell 125:59-69. 2006..Significantly, when both Bqt1 and Bqt2 are ectopically expressed in mitotic cells, they also form a bridge between Rap1 and Sad1. Thus, a complex including Bqt1 and Bqt2 is essential for connecting telomeres to the SPB... - Meiosis-specific noncoding RNA mediates robust pairing of homologous chromosomes in meiosisDa Qiao Ding
Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology, Kobe 651 2492, Japan
Science 336:732-6. 2012..Thus, we propose that RNA transcripts retained on the chromosome play an active role in recognition of homologous chromosomes for pairing... - Meiosis induced by inactivation of Pat1 kinase proceeds with aberrant nuclear positioning of centromeres in the fission yeast Schizosaccharomyces pombeYuji Chikashige
Cell Biology Group, Kansai Advanced Research Center, National Institute of Information and Communications Technology, 588-2 Iwaoka, Iwaoka-cho, Nishi-ku, Kobe 651-2492, Japan
Genes Cells 9:671-84. 2004..As Pat1 inactivation is often useful to induce S. pombe cells synchronously into meiosis, the temporal order of chromosomal events determined here will provide landmarks for the progression of meiosis downstream the Pat1 inactivation... - Gene expression and distribution of Swi6 in partial aneuploids of the fission yeast Schizosaccharomyces pombeYuji Chikashige
Cell Biology Project, Biological ICT Group, Kobe Advanced ICT Research Center, National Institute of Information and Communications Technology, and CREST Research Project, Kobe, Japan
Cell Struct Funct 32:149-61. 2007..These results suggest that additional chromosomes in aneuploids could lead to imbalances in gene expression through changes in distribution of heterochromatin as well as in gene dosage... - Meiotic cohesins modulate chromosome compaction during meiotic prophase in fission yeastDa Qiao Ding
Cell Biology Group, Kansai Advanced Research Center, National Institute of Information and Communications Technology, Kobe 651 2492, Japan
J Cell Biol 174:499-508. 2006.... - Nuclear localization of barrier-to-autointegration factor is correlated with progression of S phase in human cellsTokuko Haraguchi
CREST Research Project, Kansai Advanced Research Center, National Institute of Information and Communications Technology, 588 2 Iwaoka, Iwaoka cho, Nishi ku, Kobe 651 2492, Japan
J Cell Sci 120:1967-77. 2007..BAF downregulation also caused lamin A to mislocalize away from the nuclear envelope. These results indicate that BAF is required for the integrity of the nuclear lamina and normal progression of S phase in human cells... - Modulation of Alp4 function in Schizosaccharomyces pombe induces novel phenotypes that imply distinct functions for nuclear and cytoplasmic gamma-tubulin complexesHirohisa Masuda
Cell Biology Group and CREST JST, Kansai Advanced Research Center, National Institute of Information and Communications Technology, Kobe, 651 2492, Japan
Genes Cells 11:319-36. 2006.... - Dissociation of the Nuf2-Ndc80 complex releases centromeres from the spindle-pole body during meiotic prophase in fission yeastHaruhiko Asakawa
Cell Biology Group and CREST Research Project, Kansai Advanced Research Center, National Institute of Information and Communications Technology, Kobe 651 2492, Japan
Mol Biol Cell 16:2325-38. 2005.... - Spectral imaging fluorescence microscopyTokuko Haraguchi
CREST Research Project, Kansai Advanced Research Center, Communications Research Laboratory, 588-2 Iwaoka, Iwaoka-cho, Nishi-ku, Kobe 651-2492, Japan
Genes Cells 7:881-7. 2002.... - Multispectral imaging fluorescence microscopy for living cellsYasushi Hiraoka
Kansai Advanced Research Center, Communications Research Laboratory, Kobe, Japan
Cell Struct Funct 27:367-74. 2002..We introduce these systems with particular interest in live cell imaging. Finally we demonstrate examples of spectral imaging of living cells using commercially available systems with no need for user engineering... - Artificial induction of autophagy around polystyrene beads in nonphagocytic cellsShouhei Kobayashi
Kobe Advanced ICT Research Center, National Institute of Information and Communications Technology, Iwaoka cho, Nishi ku, Kobe, Japan
Autophagy 6:36-45. 2010..In addition, the beads were subsequently transferred to lysosomes within a couple of hours. Our findings demonstrate the cellular responses that lead to autophagy in response to pathogen invasion... - Cell cycle behavior of human HP1 subtypes: distinct molecular domains of HP1 are required for their centromeric localization during interphase and metaphaseTomohiro Hayakawa
CREST Research Project, Kansai Advanced Research Center, Communications Research Laboratory, 588-2 Iwaoka, Iwaoka-cho, Nishi-ku, Kobe 651-2492, Japan
J Cell Sci 116:3327-38. 2003.... - From meiosis to postmeiotic events: alignment and recognition of homologous chromosomes in meiosisDa Qiao Ding
Kobe Advanced ICT Research Center, National Institute of Information and Communications Technology, Kobe, Japan
FEBS J 277:565-70. 2010..Here, we discuss the various mechanisms used for alignment and recognition of homologous chromosomes in meiosis... - Two distinct repeat sequences of Nup98 nucleoporins characterize dual nuclei in the binucleated ciliate tetrahymenaMasaaki Iwamoto
Kobe Advanced ICT Research Center, National Institute of Information and Communications Technology, Kobe 651 2492, Japan
Curr Biol 19:843-7. 2009..These results suggest that Nup98s act as a barrier to misdirected localization of nucleus-specific proteins. Our findings provide the first evidence that the NPC contributes to nucleus-selective transport in ciliates... - Dynamics of homologous chromosome pairing during meiotic prophase in fission yeastDa-Qiao Ding
CREST Research Project, Kansai Advanced Research Center, 588-2 Iwaoka, Iwaoka-cho, Nishi-ku, Kobe 651-2492, Japan
Dev Cell 6:329-41. 2004.... - Another way to move chromosomesYuji Chikashige
Kobe Advanced ICT Research Center, National Institute of Information and Communications Technology, 588 2 Iwaoka, Iwaoka cho, Nishi ku, Kobe, 651 2492, Japan
Chromosoma 116:497-505. 2007..Telomeres are then moved along the nuclear envelope using cytoplasmic microtubules. These findings illustrate a general mechanism for transmitting a cytoskeletal driving force to chromosomes across the nuclear envelope... - Early entry and deformation of macropinosomes correlates with high efficiency of decaarginine-polyethylene glycol-lipid-mediated gene deliveryShouhei Kobayashi
Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology, Iwaoka, Kobe, Japan
J Gene Med 14:262-71. 2012..However, the intracellular behaviour of the two different nanostructures, which leads to differences in gene delivery, remains to be elucidated... - Chromosomally-retained RNA mediates homologous pairingDa Qiao Ding
Advanced ICT Research Institute Kobe, National Institute of Information and Communication Technology, Kobe, Japan
Nucleus 3:516-9. 2012..Acting as fiducial markers of homologous loci they would abrogate the need for direct DNA sequence homology searching... - [Fluorescence imaging to visualize molecular dynamics in living cells]Tokuko Haraguchi
Tanpakushitsu Kakusan Koso 51:1972-7. 2006 - Ect2 and MgcRacGAP regulate the activation and function of Cdc42 in mitosisFabian Oceguera-Yanez
Department of Pharmacology, Kyoto University Faculty of Medicine, Kyoto 606 8501, Japan
J Cell Biol 168:221-32. 2005..These results suggest that Ect2 and MgcRacGAP regulate the activation and function of Cdc42 in mitosis... - LAP2alpha and BAF transiently localize to telomeres and specific regions on chromatin during nuclear assemblyThomas Dechat
Max F Perutz Laboratories, University Departments at the Vienna Biocenter, Department of Medical Biochemistry, Medical University of Vienna, 1030 Vienna, Austria
J Cell Sci 117:6117-28. 2004..We propose that LAP2alpha and a subfraction of BAF form defined complexes in chromatin core regions and may be involved in chromatin reorganization during early stages of nuclear assembly... - Emerin is hyperphosphorylated and redistributed in herpes simplex virus type 1-infected cells in a manner dependent on both UL34 and US3Natalie Leach
Department of Microbiology, The University of Iowa, 3 432 Bowen Science Building, Iowa City, IA 52242, USA
J Virol 81:10792-803. 2007.... - Interaction of the chromatin compaction-inducing domain (LR domain) of Ki-67 antigen with HP1 proteinsAi Kametaka
Department of Cell Biology and Neuroscience, Graduate School of Medicine, Osaka University, 2 2 Yamada oka, Suita, Japan
Genes Cells 7:1231-42. 2002..The possible human homologue of chmadrin, Ki-67 antigen (pKi-67), also has a stretch of LR pairs, but with no obvious overall similarity, at its C-terminus... - Dynamic behavior of Nuf2-Hec1 complex that localizes to the centrosome and centromere and is essential for mitotic progression in vertebrate cellsTetsuya Hori
PRESTO, The Japan Science and Technology Corporation, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima, Shizuoka 411 8540, Japan
J Cell Sci 116:3347-62. 2003..Furthermore, photobleaching experiments revealed that the Nuf2-Hec1 complex is stably associated with the centromere and that interaction of this complex with the centrosome is dynamic... - Peroxisomes are formed from complex membrane structures in PEX6-deficient CHO cells upon genetic complementationNoriyo Hashiguchi
Department of Life Science, Faculty of Science, Himeji Institute of Technology, Kamigori, Hyogo 678-1297, Japan
Mol Biol Cell 13:711-22. 2002..Our results indicate that 1) Peroxisomal ghosts are complex membrane structures; and 2) The complex membrane structures become import competent and are converted into peroxisomes upon complementation with PEX6... - The constitutive centromere component CENP-50 is required for recovery from spindle damageYukinori Minoshima
National Institute of Genetics, Mishima, Shizuoka 411 8540, Japan
Mol Cell Biol 25:10315-28. 2005..We also observed severe mitotic defects in CENP-50-deficient cells with apparent premature sister chromatid separation when the mitotic checkpoint was activated, indicating that CENP-50 is required for recovery from spindle damage... - Heat-shock induced nuclear retention and recycling inhibition of importin alphaMaiko Furuta
Cellular Dynamics Laboratory, Discovery Research Institute, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
Genes Cells 9:429-41. 2004..This suggests a new regulatory mechanism for the adaptation of cells to environmental changes, such as heat-shock... - Cellular stresses induce the nuclear accumulation of importin alpha and cause a conventional nuclear import blockYoichi Miyamoto
Department of Frontier Biosciences, Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamada-oka, Suita, Osaka 565-0871, Japan
J Cell Biol 165:617-23. 2004..These findings suggest that the stress-induced nuclear accumulation of importin alpha can be involved in a common physiological response to various stress conditions... - Functional domain analysis of human HP1 isoforms in DrosophilaMasaki Kato
Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo ku, Kyoto, Japan
Cell Struct Funct 32:57-67. 2007..These results suggest human HP1 subtypes use different domains to suppress gene expression in Drosophila cells... - [Dynamics of the nuclear envelope in the cell cycle]Shouhei Kobayashi
Tanpakushitsu Kakusan Koso 51:2165-71. 2006 - Nuclear structure-associated TIF2 recruits glucocorticoid receptor and its target DNAHidesato Ogawa
Department of Biochemistry, Institute for Virus Research, Kyoto University, Japan
Biochem Biophys Res Commun 320:218-25. 2004..The TIF2 foci could recruit GR carrying a microinjected GR responsive element. We propose that TIF2 provides a nuclear compartment that allows the assembly of multi-protein complexes required for GR-mediated gene activation... - The assembly and maintenance of heterochromatin initiated by transgene repeats are independent of the RNA interference pathway in mammalian cellsFangwei Wang
Genomics Research Institute, Utsunomiya University, 350 Mine-machi, Utsunomiya, Tochigi 321-8505, Japan
Mol Cell Biol 26:4028-40. 2006..Our results suggest that the RNAi pathway is not required for the assembly and maintenance of noncentromeric heterochromatin initiated by tandem transgene repeats in mammalian cells...