Kohji Ito

Summary

Affiliation: Chiba University
Country: Japan

Publications

  1. ncbi request reprint Kinetic mechanism of the fastest motor protein, Chara myosin
    Kohji Ito
    Department of Biology, Chiba University, Inage Ku, Chiba 263 8522, Japan
    J Biol Chem 282:19534-45. 2007
  2. pmc Unique charge distribution in surface loops confers high velocity on the fast motor protein Chara myosin
    Kohji Ito
    Department of Biology, Chiba University, Inage Ku, Chiba City, Chiba 263 8522, Japan
    Proc Natl Acad Sci U S A 106:21585-90. 2009
  3. ncbi request reprint Recombinant motor domain constructs of Chara corallina myosin display fast motility and high ATPase activity
    Kohji Ito
    Department of Biology, Chiba University, Inage Ku, 263 8522, Chiba, Japan
    Biochem Biophys Res Commun 312:958-64. 2003
  4. ncbi request reprint Roles of the hydrophobic triplet in the motor domain of myosin in the interaction between myosin and actin
    You Hachikubo
    Department of Biology, Chiba University, 1 33 Yayoicho, Inage Ku, Chiba 263 8522
    J Biochem 134:165-71. 2003
  5. ncbi request reprint Importance of the converter region for the motility of myosin as revealed by the studies on chimeric Chara myosins
    Masaya Seki
    Department of Biology, Chiba University, Inage Ku, 263 8522 Chiba, Japan
    J Mol Biol 344:311-5. 2004
  6. ncbi request reprint Enzymatic activity and motility of recombinant Arabidopsis myosin XI, MYA1
    You Hachikubo
    Department of Biology, Chiba University, Inageku, Chiba, 263 8522 Japan
    Plant Cell Physiol 48:886-91. 2007
  7. ncbi request reprint Binding of chara Myosin globular tail domain to phospholipid vesicles
    Shun Ya Nunokawa
    Department of Biology, Chiba University, Yayoicho, Inage Ku, Chiba, 263 8522 Japan
    Plant Cell Physiol 48:1558-66. 2007
  8. ncbi request reprint Requirement of domain-domain interaction for conformational change and functional ATP hydrolysis in myosin
    Kohji Ito
    Department of Biology, Chiba University, Inage Ku, Chiba 263 8522, Japan
    J Biol Chem 278:31049-57. 2003
  9. ncbi request reprint Susceptibility of Chara myosin to SH reagents
    Masaya Seki
    Department of Biology, Chiba University, 1 33 Yayoi cho, Inage Ku, Chiba, 263 8522 Japan
    Plant Cell Physiol 44:201-5. 2003
  10. ncbi request reprint The motility of Chara corallina myosin was inhibited reversibly by 2,3-butanedione monoxime (BDM)
    Keisuke Funaki
    Department of Biology, Chiba University, Inage Ku, Chiba, 263 8522 Japan
    Plant Cell Physiol 45:1342-5. 2004

Collaborators

Detail Information

Publications14

  1. ncbi request reprint Kinetic mechanism of the fastest motor protein, Chara myosin
    Kohji Ito
    Department of Biology, Chiba University, Inage Ku, Chiba 263 8522, Japan
    J Biol Chem 282:19534-45. 2007
    ..In addition, this study reveals some striking kinetic features of Chara myosin that are suited for the fast movement: a dramatic acceleration of ADP release by actin (1000-fold) and extremely fast ATP binding rate...
  2. pmc Unique charge distribution in surface loops confers high velocity on the fast motor protein Chara myosin
    Kohji Ito
    Department of Biology, Chiba University, Inage Ku, Chiba City, Chiba 263 8522, Japan
    Proc Natl Acad Sci U S A 106:21585-90. 2009
    ..Removing positive charge from loop 2 and adding positive charge to loop 3 of Dictyostelium myosin made its velocity higher than that of the wild type, suggesting that the charge strategy in loops 2 and 3 is widely applicable...
  3. ncbi request reprint Recombinant motor domain constructs of Chara corallina myosin display fast motility and high ATPase activity
    Kohji Ito
    Department of Biology, Chiba University, Inage Ku, 263 8522, Chiba, Japan
    Biochem Biophys Res Commun 312:958-64. 2003
    ..Our results provide an excellent system to dissect the specific structural and functional features that distinguish the myosin responsible for fast cytoplasmic streaming...
  4. ncbi request reprint Roles of the hydrophobic triplet in the motor domain of myosin in the interaction between myosin and actin
    You Hachikubo
    Department of Biology, Chiba University, 1 33 Yayoicho, Inage Ku, Chiba 263 8522
    J Biochem 134:165-71. 2003
    ....
  5. ncbi request reprint Importance of the converter region for the motility of myosin as revealed by the studies on chimeric Chara myosins
    Masaya Seki
    Department of Biology, Chiba University, Inage Ku, 263 8522 Chiba, Japan
    J Mol Biol 344:311-5. 2004
    ..6-fold without changing the ATPase activity so much. The result clearly showed the importance of interaction between the converter region and the lever arm alpha-helix for the efficient motility of myosin...
  6. ncbi request reprint Enzymatic activity and motility of recombinant Arabidopsis myosin XI, MYA1
    You Hachikubo
    Department of Biology, Chiba University, Inageku, Chiba, 263 8522 Japan
    Plant Cell Physiol 48:886-91. 2007
    ..This high ratio of actin-bound states is also a characteristic of processive motors. Our results strongly suggest that MYA1 is a processive motor and involved in vesicle transport and/or cytoplasmic streaming...
  7. ncbi request reprint Binding of chara Myosin globular tail domain to phospholipid vesicles
    Shun Ya Nunokawa
    Department of Biology, Chiba University, Yayoicho, Inage Ku, Chiba, 263 8522 Japan
    Plant Cell Physiol 48:1558-66. 2007
    ..We also suggest a possible regulatory mechanism of cytoplasmic streaming including phosphorylation-dependent dissociation of the globular tail domain from the endoplasmic reticulum membrane...
  8. ncbi request reprint Requirement of domain-domain interaction for conformational change and functional ATP hydrolysis in myosin
    Kohji Ito
    Department of Biology, Chiba University, Inage Ku, Chiba 263 8522, Japan
    J Biol Chem 278:31049-57. 2003
    ..Thus, the contact between these two side chains appears to play an important role in the coordinated conformational changes and subsequent ATP hydrolysis...
  9. ncbi request reprint Susceptibility of Chara myosin to SH reagents
    Masaya Seki
    Department of Biology, Chiba University, 1 33 Yayoi cho, Inage Ku, Chiba, 263 8522 Japan
    Plant Cell Physiol 44:201-5. 2003
    ..corallina decreased to a level accountable for the decrease in the velocity of cytoplasmic streaming but it was also found that Chara myosin was far less susceptible to NEM than skeletal muscle myosin...
  10. ncbi request reprint The motility of Chara corallina myosin was inhibited reversibly by 2,3-butanedione monoxime (BDM)
    Keisuke Funaki
    Department of Biology, Chiba University, Inage Ku, Chiba, 263 8522 Japan
    Plant Cell Physiol 45:1342-5. 2004
    ..These results suggested that effect of BDM on cytoplasmic streaming was exerted through myosin and not through ion channels at least in Chara corallina, though a very high concentration of BDM was required...
  11. doi request reprint Function of the head-tail junction in the activity of myosin II
    Takeshi Haraguchi
    Department of Biology, Graduate School of Science, Chiba University, Inage Ku, Chiba 263 8522, Japan
    Biochem Biophys Res Commun 440:490-4. 2013
    ..Our results suggest that the first leucine of the conserved Leu-Leu sequence at the head-tail junction profoundly affects the cooperativity between the two heads involved in the actin activated ATPase activity of myosin II. ..
  12. ncbi request reprint Some motile properties of fast characean myosin
    Jun ya Awata
    Department of Biology, Chiba University, Inage Ku, 263 8522, Chiba, Japan
    J Mol Biol 326:659-63. 2003
    ..The motor density required to produce a successful movement of actin filament was about 200 molecules/microm(2). These results suggest that the characean myosin is not a processive motor protein...
  13. pmc Molecular Characterization and Subcellular Localization of Arabidopsis Class VIII Myosin, ATM1
    Takeshi Haraguchi
    From the Department of Biology, Graduate School of Science, Chiba University, Inage Ku, Chiba 263 8522
    J Biol Chem 289:12343-55. 2014
    ..Our results suggest that ATM1 functions as a tension sensor/generator at the cell cortex and other structures in Arabidopsis. ..
  14. ncbi request reprint Kinetic characterization of tail swing steps in the ATPase cycle of Dictyostelium cytoplasmic dynein
    Toshifumi Mogami
    Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Komaba 3 8 1, Tokyo, Japan
    J Biol Chem 282:21639-44. 2007
    ....