Minoru Nakao

Summary

Affiliation: Asahikawa Medical College
Country: Japan

Publications

  1. ncbi request reprint Mitochondrial genetic code in cestodes
    M Nakao
    Department of Parasitology, Asahikawa Medical College, Hokkaido, Japan
    Mol Biochem Parasitol 111:415-24. 2000
  2. ncbi request reprint Genetic variation in Taenia solium
    Gillian Campbell
    Cestode Zoonoses Research Group, Bioscience Research Institute and School of Environment and Life Sciences, Salford University, UK
    Parasitol Int 55:S121-6. 2006
  3. ncbi request reprint Phylogenetic systematics of the genus Echinococcus (Cestoda: Taeniidae)
    Minoru Nakao
    Department of Parasitology, Asahikawa Medical University, Asahikawa, Midorigaoka Higashi 2 1, Hokkaido 078, Japan Electronic address
    Int J Parasitol 43:1017-29. 2013
  4. doi request reprint Molecular phylogeny of the genus Taenia (Cestoda: Taeniidae): proposals for the resurrection of Hydatigera Lamarck, 1816 and the creation of a new genus Versteria
    Minoru Nakao
    Department of Parasitology, Asahikawa Medical University, Asahikawa, Hokkaido 078 8510, Japan
    Int J Parasitol 43:427-37. 2013
  5. pmc Genetic polymorphisms of Echinococcus tapeworms in China as determined by mitochondrial and nuclear DNA sequences
    Minoru Nakao
    Department of Parasitology, Asahikawa Medical College, Asahikawa, Hokkaido 078 8510, Japan
    Int J Parasitol 40:379-85. 2010
  6. ncbi request reprint Mitochondrial genomes of the human broad tapeworms Diphyllobothrium latum and Diphyllobothrium nihonkaiense (Cestoda: Diphyllobothriidae)
    Minoru Nakao
    Department of Parasitology, Asahikawa Medical College, Midorigaoka Higashi 2 1, Asahikawa, Hokkaido 078 8510, Japan
    Parasitol Res 101:233-6. 2007
  7. ncbi request reprint A molecular phylogeny of the genus Echinococcus inferred from complete mitochondrial genomes
    M Nakao
    Department of Parasitology, Asahikawa Medical College, Asahikawa, Hokkaido 078 8510, Japan
    Parasitology 134:713-22. 2007
  8. ncbi request reprint The mitochondrial genome of the tapeworm Taenia solium: a finding of the abbreviated stop codon U
    Minoru Nakao
    Department of Parasitology, Asahikawa Medical College, Asahikawa 078 8510, Japan
    J Parasitol 89:633-5. 2003
  9. ncbi request reprint Geographic pattern of genetic variation in the fox tapeworm Echinococcus multilocularis
    Minoru Nakao
    Department of Parasitology, Asahikawa Medical College, Asahikawa, Hokkaido 078 8510, Japan
    Parasitol Int 58:384-9. 2009
  10. ncbi request reprint Isolation of polymorphic microsatellite loci from the tapeworm Echinococcus multilocularis
    Minoru Nakao
    Department of Parasitology, Asahikawa Medical College, Asahikawa, Hokkaido 078 8510, Japan
    Infect Genet Evol 3:159-63. 2003

Collaborators

Detail Information

Publications86

  1. ncbi request reprint Mitochondrial genetic code in cestodes
    M Nakao
    Department of Parasitology, Asahikawa Medical College, Hokkaido, Japan
    Mol Biochem Parasitol 111:415-24. 2000
    ..Based on these results, we propose that the flatworm mitochondrial code should be modified for cestodes, particularly, in an initiating methionine codon (GUG) and a terminating codon (UAA)...
  2. ncbi request reprint Genetic variation in Taenia solium
    Gillian Campbell
    Cestode Zoonoses Research Group, Bioscience Research Institute and School of Environment and Life Sciences, Salford University, UK
    Parasitol Int 55:S121-6. 2006
    ..Preliminary studies suggest it is possible to analyse population genetic variation in communities using a range of polymorphic markers...
  3. ncbi request reprint Phylogenetic systematics of the genus Echinococcus (Cestoda: Taeniidae)
    Minoru Nakao
    Department of Parasitology, Asahikawa Medical University, Asahikawa, Midorigaoka Higashi 2 1, Hokkaido 078, Japan Electronic address
    Int J Parasitol 43:1017-29. 2013
    ..is also presented. The taxonomic nomenclature for Echinococcus oligarthrus is shown to be incorrect and this name should be replaced with Echinococcus oligarthra. ..
  4. doi request reprint Molecular phylogeny of the genus Taenia (Cestoda: Taeniidae): proposals for the resurrection of Hydatigera Lamarck, 1816 and the creation of a new genus Versteria
    Minoru Nakao
    Department of Parasitology, Asahikawa Medical University, Asahikawa, Hokkaido 078 8510, Japan
    Int J Parasitol 43:427-37. 2013
    ..nov., although molecular evidence is not available. Taenia taeniaeformis has been historically regarded as a single species but the present data clearly demonstrate that it consists of two cryptic species...
  5. pmc Genetic polymorphisms of Echinococcus tapeworms in China as determined by mitochondrial and nuclear DNA sequences
    Minoru Nakao
    Department of Parasitology, Asahikawa Medical College, Asahikawa, Hokkaido 078 8510, Japan
    Int J Parasitol 40:379-85. 2010
    ..shiquicus was segregated into the Tibetan Plateau by colonising alpine mammals and its mitochondrial locus has evolved without bottleneck effects...
  6. ncbi request reprint Mitochondrial genomes of the human broad tapeworms Diphyllobothrium latum and Diphyllobothrium nihonkaiense (Cestoda: Diphyllobothriidae)
    Minoru Nakao
    Department of Parasitology, Asahikawa Medical College, Midorigaoka Higashi 2 1, Asahikawa, Hokkaido 078 8510, Japan
    Parasitol Res 101:233-6. 2007
    ..nihonkaiense is a distinct species. The sequences determined in this study may provide useful marker systems for diagnostic, epidemiological, and phylogeographical studies of human diphyllobothriasis...
  7. ncbi request reprint A molecular phylogeny of the genus Echinococcus inferred from complete mitochondrial genomes
    M Nakao
    Department of Parasitology, Asahikawa Medical College, Asahikawa, Hokkaido 078 8510, Japan
    Parasitology 134:713-22. 2007
    ..Host-parasite co-evolution comparisons suggest that the ancestral homeland of Echinococcus was North America or Asia, depending on whether the ancestral definitive hosts were canids or felids...
  8. ncbi request reprint The mitochondrial genome of the tapeworm Taenia solium: a finding of the abbreviated stop codon U
    Minoru Nakao
    Department of Parasitology, Asahikawa Medical College, Asahikawa 078 8510, Japan
    J Parasitol 89:633-5. 2003
    ..In a gene for NADH dehydrogenase subunit 1, the abbreviated stop codon U was confirmed for the first time in flatworm mtDNAs...
  9. ncbi request reprint Geographic pattern of genetic variation in the fox tapeworm Echinococcus multilocularis
    Minoru Nakao
    Department of Parasitology, Asahikawa Medical College, Asahikawa, Hokkaido 078 8510, Japan
    Parasitol Int 58:384-9. 2009
    ..The nuclear DNA sequence for the immunodominant B cell epitope region of ezrin/radixin/moesin-like protein (elp) was extremely conservative, indicating that the elp antigen is available for immunodiagnosis in any endemic areas...
  10. ncbi request reprint Isolation of polymorphic microsatellite loci from the tapeworm Echinococcus multilocularis
    Minoru Nakao
    Department of Parasitology, Asahikawa Medical College, Asahikawa, Hokkaido 078 8510, Japan
    Infect Genet Evol 3:159-63. 2003
    ..We found four alleles of EMms1 and two alleles of EMms2. The heterozygosities observed were 10.6% in EMms1 and 7.7% in EMms2. The result suggests that both selfing and outcrossing occur in the adult stage of E. multilocularis...
  11. ncbi request reprint Taeniasis and cysticercosis in Asia and the Pacific: present state of knowledge and perspectives
    Akira Ito
    Department of Parasitology, Asahikawa Medical College, Midorigaoka Higashi 2 1 1 1, Asahikawa 078 8510, Japan
    Southeast Asian J Trop Med Public Health 36:123-30. 2005
    ..saginata morphologically, it is time to re-evaluate T. saginata in Asia and the Pacific. New and broad-based surveys across this region are necessary from epidemiological and public health perspectives, based on evidence...
  12. ncbi request reprint Echinococcus multilocularis: developmental stage-specific expression of Antigen B 8-kDa-subunits
    Wulamu Mamuti
    Department of Parasitology, Asahikawa Medical College, Japan
    Exp Parasitol 113:75-82. 2006
    ..multilocularis vesicles, protoscoleces, and immature adult worms. Possible functions of different expression manners are also discussed...
  13. pmc Evaluation of use of recombinant Em18 and affinity-purified Em18 for serological differentiation of alveolar echinococcosis from cystic echinococcosis and other parasitic infections
    Ning Xiao
    Department of Parasitology, Asahikawa Medical College, Asahikawa, Japan and Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
    J Clin Microbiol 41:3351-3. 2003
    ..The results indicate that rEm18 is highly useful for serodiagnosis...
  14. ncbi request reprint Molecular characterization of a novel gene encoding an 8-kDa-subunit of antigen B from Echinococcus granulosus genotypes 1 and 6
    Wulamu Mamuti
    Department of Parasitology, Asahikawa Medical College, Midorigaoka Higashi 2 1 1 1, Asahikawa 078 8510, Japan
    Parasitol Int 56:313-6. 2007
    ..granulosus genotypes 1 and 6 by PCR, and named as EgG1AgB8/5 and EgG6AgB8/5, respectively. The phylogenetic relationship of these genes with other genes encoding the antigen B 8-kDa-subunit monomers was also discussed...
  15. ncbi request reprint Evaluation of purified Taenia solium glycoproteins and recombinant antigens in the serologic detection of human and swine cysticercosis
    Marcello Otake Sato
    Department of Parasitology, Asahikawa Medical College, Asahikawa Hokkaido, Japan
    J Infect Dis 194:1783-90. 2006
    ..In addition, the conservation of the genes encoding Ag1V1 and Ag2 in T. solium worldwide was verified. These results indicate that Rec-Ag1V1/Ag2 has great potential for usefulness in serodiagnosis as an alternative to native antigens...
  16. ncbi request reprint Towards the international collaboration for detection, surveillance and control of taeniasis/ cysticercosis and echinococcosis in Asia and the Pacific
    Akira Ito
    Department of Parasitology, Asahikawa Medical College, Asahikawa, Japan
    Southeast Asian J Trop Med Public Health 37:82-90. 2006
    ....
  17. ncbi request reprint Short report: Identification of Echinococcus species from a yak in the Qinghai-Tibet plateau region of China
    Ning Xiao
    Department of Parasitology and Animal Laboratory for Medical Research, Asahikawa Medical College, Asahikawa, Japan
    Am J Trop Med Hyg 69:445-6. 2003
    ..Nucleotide sequences of the cytochrome c oxidase subunit 1 (cox1) and cytochrome b (cytb) genes amplified from the echinococcal lesion demonstrated that the yak was infected with the E. granulosus G1 genotype (sheep strain)...
  18. ncbi request reprint Development of Em18-immunoblot and Em18-ELISA for specific diagnosis of alveolar echinococcosis
    Akira Ito
    Department of Parasitology, Asahikawa Medical College, Midorigaoka Higashi 2 1 1 1, 078 8510, Asahikawa, Japan
    Acta Trop 85:173-82. 2003
    ....
  19. pmc Loop-mediated isothermal amplification method for differentiation and rapid detection of Taenia species
    Agathe Nkouawa
    Department of Parasitology, Asahikawa Medical College, Midorigaoka Higashi 2 1 1 1, Asahikawa 078 8510, Hokkaido, Japan
    J Clin Microbiol 47:168-74. 2009
    ....
  20. ncbi request reprint Usefulness of severe combined immunodeficiency (scid) and inbred mice for studies of cysticercosis and echinococcosis
    Kazuhiro Nakaya
    Animal Laboratory for Medical Research, Asahikawa Medical College, Hokkaido, Japan
    Parasitol Int 55:S91-7. 2006
    ..The advantages of a mouse model for Echinococcus granulosus are also described...
  21. doi request reprint Evidence of hybridization between Taenia saginata and Taenia asiatica
    Munehiro Okamoto
    Department of Parasitology, School of Veterinary Medicine, Tottori University, Japan
    Parasitol Int 59:70-4. 2010
    ..saginata. These findings are evidence of occasional hybridization between the two species, although the possibility of retention of ancestral polymorphism cannot be excluded...
  22. ncbi request reprint Cysticercosis/taeniasis in Asia and the Pacific
    Akira Ito
    Department of Parasitology, Asahikawa Medical College, Japan
    Vector Borne Zoonotic Dis 4:95-107. 2004
    ....
  23. ncbi request reprint Echinococcus shiquicus n. sp., a taeniid cestode from Tibetan fox and plateau pika in China
    Ning Xiao
    Department of Parasitology, Asahikawa Medical College, Japan
    Int J Parasitol 35:693-701. 2005
    ..Hydatid cysts of E. shiquicus found in the livers from the pikas were essentially unilocular but an oligovesicular cyst was also found. The data of mitochondrial and nuclear DNA sequences proved E. shiquicus to be a valid taxon...
  24. pmc DNA differential diagnosis of taeniasis and cysticercosis by multiplex PCR
    Hiroshi Yamasaki
    Department of Parasitology Animal Laboratory for Medical Research, Asahikawa Medical College, Asahikawa, Japan
    J Clin Microbiol 42:548-53. 2004
    ..saginata carriers. The application of the multiplex PCR would be useful not only for surveillance of taeniasis and cysticercosis control but also for the molecular epidemiological survey of these cestode infections...
  25. ncbi request reprint Echinococcus species from red foxes, corsac foxes, and wolves in Mongolia
    Akira Ito
    Department of Parasitology, Asahikawa Medical University, Asahikawa, Hokkaido, Japan
    Parasitology 140:1648-54. 2013
    ..The genotypes of E. multilocularis and E. canadensis are discussed in terms of host specificity and distribution in Mongolia. The importance of wolves in the completion of the life cycle of Echinococcus spp. is also discussed...
  26. pmc Molecular cloning, expression, and serological evaluation of an 8-kilodalton subunit of antigen B from Echinococcus multilocularis
    Wulamu Mamuti
    Department of Parasitology, Asahikawa Medical College, Asahikawa, Japan
    J Clin Microbiol 42:1082-8. 2004
    ..There was no cross-reaction with sera from patients with cysticercosis, which often cross-react when native antigens are used for serodiagnosis...
  27. ncbi request reprint Recent hybridization between Taenia asiatica and Taenia saginata
    Kanako Yamane
    Department of Parasitology, School of Veterinary Medicine, Faculty of Agriculture, Tottori University, Japan
    Parasitol Int 61:351-5. 2012
    ..Hybridization between T. asiatica and T. saginata is probably an ongoing event in many areas in which they are sympatrically endemic...
  28. ncbi request reprint A loop-mediated isothermal amplification method for a differential identification of Taenia tapeworms from human: application to a field survey
    Agathe Nkouawa
    Department of Parasitology, Asahikawa Medical University, Midorigaoka Higashi 2 1 1 1, Asahikawa, 078 8510, Hokkaido, Japan
    Parasitol Int 61:723-5. 2012
    ..These results demonstrated that the LAMP method enabled rapid identification of parasites in the field surveys, which suggested that this method would contribute to the control of Taenia infections in endemic areas...
  29. pmc Evaluation of a loop-mediated isothermal amplification method using fecal specimens for differential detection of Taenia species from humans
    Agathe Nkouawa
    Department of Parasitology, Asahikawa Medical College, Midorigaoka Higashi 2 1 1 1, Asahikawa 078 8510, Hokkaido, Japan
    J Clin Microbiol 48:3350-2. 2010
    ..The LAMP method, with no false positives, showed a higher sensitivity (88.4%) than the multiplex PCR (37.2%). Thus, it is expected that the LAMP method has a high value for molecular diagnosis of taeniasis...
  30. pmc Solitary neurocysticercosis case caused by Asian genotype of Taenia solium confirmed by mitochondrial DNA analysis
    Hiroshi Yamasaki
    Department of Parasitology, Asahikawa Medical College, Midorigaoka Higashi 2 1 1 1, Asahikawa 078 8510, Japan
    J Clin Microbiol 42:3891-3. 2004
    ..However, the illness was confirmed by mitochondrial DNA analysis to be a solitary neurocysticercosis case caused by the Asian genotype of Taenia solium...
  31. pmc Evaluation of an enzyme-linked immunosorbent assay (ELISA) with affinity-purified Em18 and an ELISA with recombinant Em18 for differential diagnosis of alveolar echinococcosis: results of a blind test
    Akira Ito
    Department of Parasitology Animal Laboratory for Medical Research, Asahikawa Medical College, Asahikawa, Japan
    J Clin Microbiol 40:4161-5. 2002
    ....
  32. ncbi request reprint Echinococcosis and cysticercosis in Asia: evaluation of the modern technology for epidemiological study
    Akira Ito
    Department of Parasitology, Asahikawa Medical College, Asahikawa, Japan
    Southeast Asian J Trop Med Public Health 34:103-7. 2003
    ..In the present review, we introduce such technologies applied in Japan, China and Indonesia, and point out the problems that need to be solved for control of these three zoonotic cestodiases...
  33. ncbi request reprint Mitochondrial phylogeny of the genus Echinococcus (Cestoda: Taeniidae) with emphasis on relationships among Echinococcus canadensis genotypes
    Minoru Nakao
    Department of Parasitology, Asahikawa Medical University, Asahikawa, Hokkaido 078 8510, Japan
    Parasitology 140:1625-36. 2013
    ..canadensis strains have been important for epidemiological considerations. However, the taxonomic treatment of the strains as separate species or subspecies is invalid from a molecular cladistic viewpoint...
  34. ncbi request reprint Dual infection of animal hosts with different Echinococcus species in the eastern Qinghai-Tibet plateau region of China
    Ning Xiao
    Department of Parasitology, Asahikawa Medical College, Asahikawa, Japan
    Am J Trop Med Hyg 75:292-4. 2006
    ..shiquicus in the liver. The high susceptibility of some mammalian hosts to the parasites and the high prevalence of the three co-endemic species probably increase the chance of mixed infections in the eastern Tibetan plateau...
  35. ncbi request reprint The Tibetan hare Lepus oiostolus: a novel intermediate host for Echinococcus multilocularis
    Ning Xiao
    Department of Parasitology, Asahikawa Medical College, Midorigaoka Higashi 2 1, 078 8510 Asahikawa, Japan
    Parasitol Res 92:352-3. 2004
    ..This is the first confirmation of larval E. multilocularis from hares. The hydatids contained protoscolices, suggesting that the hare may contribute to the transmission of E. multilocularis on the Tibetan plateau...
  36. ncbi request reprint A case of intramuscular cysticercosis diagnosed definitively by mitochondrial DNA analysis of extremely calcified cysts
    Hiroshi Yamasaki
    Department of Parasitology, Asahikawa Medical College, Asahikawa 078 8510, Japan
    Parasitol Int 55:127-30. 2006
    ..Mitochondrial DNA analysis of a histopathological specimen confirmed the causative agent to be the Asian genotype of T. solium...
  37. ncbi request reprint Mitochondrial DNA diagnosis for taeniasis and cysticercosis
    Hiroshi Yamasaki
    Department of Parasitology, Asahikawa Medical College, Japan
    Parasitol Int 55:S81-5. 2006
    ..Moreover, molecular diagnosis of cysticerci found in specimens submitted for histopathology and the molecular detection of taeniasis using copro-DNA were discussed...
  38. ncbi request reprint Recent advances in characterization of Echinococcus antigen B
    Wulamu Mamuti
    Department of Parasitology, Asahikawa Medical College, Japan
    Parasitol Int 55:S57-62. 2006
    ..This review focuses on recent advances in molecular biological and immunological characterization of AgB from both of E. granulosus and E. multilocularis...
  39. ncbi request reprint Recombinant antigens for serodiagnosis of cysticercosis and echinococcosis
    Yasuhito Sako
    Department of Parasitology, Asahikawa Medical College, Japan
    Parasitol Int 55:S69-73. 2006
    ..RecEm18 has the potential for use in the differential serodiagnosis of alveolar echinococcosis...
  40. ncbi request reprint Cloning and characterization of cathepsin L-like peptidases of Echinococcus multilocularis metacestodes
    Yasuhito Sako
    Department of Parasitology, Asahikawa Medical College, Midorigaoka Higashi 2 1, Asahikawa, 078 8510 Hokkaido, Japan
    Mol Biochem Parasitol 154:181-9. 2007
    ..This is the first report of cysteine peptidases from E. multilocularis, and would contribute to control E. multilocularis infections by chemotherapeutic drugs and/or immunoprophylaxis...
  41. pmc Serological monitoring of progression of alveolar echinococcosis with multiorgan involvement by use of recombinant Em18
    Yuji Ishikawa
    Department of Parasitology, Asahikawa Medical College, Midorigaoka Higashi 2 1 1 1, Asahikawa, 078 8510 Hokkaido, Japan
    J Clin Microbiol 47:3191-6. 2009
    ..An enzyme-linked immunosorbent assay for the detection of immunoglobulin G (IgG) responses, especially IgG4 responses, is expected to be a real-time indicator of the dynamics of active AE...
  42. pmc Serological studies of neurologic helminthic infections in rural areas of southwest cameroon: toxocariasis, cysticercosis and paragonimiasis
    Agathe Nkouawa
    Department of Parasitology, Asahikawa Medical College, Asahikawa, Hokkaido, Japan
    PLoS Negl Trop Dis 4:e732. 2010
    ..Soil-transmitted helminthic infections were also examined...
  43. pmc Alveolar echinococcosis: characterization of diagnostic antigen Em18 and serological evaluation of recombinant Em18
    Yasuhito Sako
    Department of Parasitology, Asahikawa Medical College, Japan
    J Clin Microbiol 40:2760-5. 2002
    ..RecEm18 has the potential for use in the differential serodiagnosis of alveolar echinococcosis...
  44. ncbi request reprint Genetic analysis of Echinococcus multilocularis originating from a patient with alveolar echinococcosis occurring in Minnesota in 1977
    Hiroshi Yamasaki
    Department of Parasitology and Animal Laboratory for Medical Research, Asahikawa Medical College, Asahikawa, Japan
    Am J Trop Med Hyg 79:245-7. 2008
    ..multilocularis isolate from Minnesota was almost identical to an isolate from South Dakota rather than isolates from contiguous Alaska...
  45. ncbi request reprint Molecular identification of Taenia solium cysticercus genotype in the histopathological specimens
    Hiroshi Yamasaki
    Department of Parasitology, Asahikawa Medical College, Asahikawa 078 8510, Japan
    Southeast Asian J Trop Med Public Health 36:131-4. 2005
    ..solium Asian genotype. When infection with T. solium is not confirmed by histopathological examination, molecular diagnosis will be more useful for definitive diagnosis...
  46. pmc DNA differential diagnosis of human taeniid cestodes by base excision sequence scanning thymine-base reader analysis with mitochondrial genes
    Hiroshi Yamasaki
    Department of Parasitology Animal Laboratory for Medical Research, Asahikawa Medical College, Midorigaoka Higashi 2 1 1 1, Asahikawa, Japan
    J Clin Microbiol 40:3818-21. 2002
    ..saginata, T. asiatica, and two genotypes of T. solium. This approach provides a useful tool for the identification and diagnosis of human taeniid cestodes without DNA sequencing if nucleotide sequence databases are available...
  47. pmc Taeniasis and cysticercosis due to Taenia solium in Japan
    Tetsuya Yanagida
    Asahikawa Medical University, Asahikawa, Hokkaido, 078 8510, Japan
    Parasit Vectors 5:18. 2012
    ..solium infections in Japan conducted in the last 15 years. Herein, we provide an overview of the status of T. solium infection in Japan over the past 35 years and point out the potential risks to Japanese society...
  48. doi request reprint Phylogenetic relationships within Echinococcus and Taenia tapeworms (Cestoda: Taeniidae): an inference from nuclear protein-coding genes
    Jenny Knapp
    Department of Parasitology, Asahikawa Medical University, Asahikawa, Hokkaido 078 8510, Japan
    Mol Phylogenet Evol 61:628-38. 2011
    ..8 Ma). Close genetic relationships among the members of Echinococcus imply that the genus is a young group in which speciation and global radiation occurred rapidly...
  49. ncbi request reprint Echinococcosis: serological detection of patients and molecular identification of parasites
    Akira Ito
    Asahikawa Medical College, Department of Parasitology, Midorigaoka, Higashi, Asahikawa, Hokkaido, Japan
    Future Microbiol 2:439-49. 2007
    ..Both the serological detection of the disease and molecular phylogeny will be discussed in perspective...
  50. ncbi request reprint Multiple genotypes of Taenia solium--ramifications for diagnosis, treatment and control
    Akira Ito
    Department of Parasitology, Asahikawa Medical College, Asahikawa 078 8510, Japan
    Acta Trop 87:95-101. 2003
    ....
  51. ncbi request reprint Human Taeniasis and cysticercosis in Asia
    Akira Ito
    Department of Parasitology, Asahikawa Medical College, Midorigaoka Higashi 2 1 1 1, 078 8510, Asahikawa, Japan
    Lancet 362:1918-20. 2003
    ..The organism is now named T asiatica, and has been found in Taiwan, Korea, China, Vietnam, and Indonesia. But it has been difficult to differentiate T saginata from beef and Asian Taenia from pork...
  52. doi request reprint Anomalous segmentation of Diphyllobothrium nihonkaiense
    Tetsuya Yanagida
    Department of Parasitology, Asahikawa Medical College, Asahikawa, Hokkaido, Japan
    Parasitol Int 59:268-70. 2010
    ..The morphology of the tapeworm did not correspond to any of the described cestodes. However, molecular identification based on nuclear and mitochondrial genes clearly showed the tapeworm was Diphyllobothrium nihonkaiense...
  53. doi request reprint State-of-the-art Echinococcus and Taenia: phylogenetic taxonomy of human-pathogenic tapeworms and its application to molecular diagnosis
    Minoru Nakao
    Department of Parasitology, Asahikawa Medical College, Midorigaoka Higashi 2 1 1 1, Asahikawa 078 8510, Japan
    Infect Genet Evol 10:444-52. 2010
    ..Here, we introduce the recent advances of taeniid phylogeny and its application to molecular diagnosis...
  54. ncbi request reprint Neurocysticercosis: assessing where the infection was acquired from
    Tetsuya Yanagida
    Department of Parasitology, Asahikawa Medical College, Hokkaido, Japan
    J Travel Med 17:206-8. 2010
    ..Mitochondrial DNA analysis strongly suggested that the patient became infected with the parasite in Nepal at least 10 years before the onset of the disease...
  55. ncbi request reprint Differential serodiagnosis of cystic and alveolar echinococcosis using native and recombinant antigens in Japan
    A Ito
    Department of Parasitology, Asahikawa Medical College, Japan
    Southeast Asian J Trop Med Public Health 32:111-5. 2001
    ....
  56. ncbi request reprint Evaluation of tongue inspection and serology for diagnosis of Taenia solium cysticercosis in swine: usefulness of ELISA using purified glycoproteins and recombinant antigen
    M O Sato
    Department of Parasitology, Asahikawa Medical College, 078 8510, Asahikawa, Japan
    Vet Parasitol 111:309-22. 2003
    ..solium from those either uninfected or infected with other taeniid species. Pigs without nodule by tongue inspection should be checked serologically in endemic areas...
  57. ncbi request reprint Cystic echinococcoses in mongolia: molecular identification, serology and risk factors
    Akira Ito
    Department of Parasitology, Asahikawa Medical University, Asahikawa, Japan
    PLoS Negl Trop Dis 8:e2937. 2014
    ..s.) is the major causative agent of CE worldwide, recent molecular epidemiological studies have revealed that E. canadensis is common in countries where camels are present. One such country is Mongolia...
  58. ncbi request reprint Genetic polymorphisms of Echinococcus granulosus sensu stricto in the Middle East
    Tetsuya Yanagida
    Department of Parasitology, Asahikawa Medical University, Asahikawa, Hokkaido 078 8510, Japan
    Parasitol Int 61:599-603. 2012
    ..To evaluate the hypothetical evolutionary scenario, further study is needed to analyze specimens from diverse host species in wider regions...
  59. ncbi request reprint Neurocysticercosis: clinical manifestation, neuroimaging, serology and molecular confirmation of histopathologic specimens
    Akira Ito
    Department of Parasitology, Asahikawa Medical College, Asahikawa, Japan
    Southeast Asian J Trop Med Public Health 37:74-81. 2006
    ..solium. Crucial confirmation is based on molecular identification. In this review, these four issues are briefly summarized...
  60. doi request reprint Paragonimiasis in Cameroon: molecular identification, serodiagnosis and clinical manifestations
    Agathe Nkouawa
    Department of Parasitology, Asahikawa Medical College, Asahikawa, Japan
    Trans R Soc Trop Med Hyg 103:255-61. 2009
    ..africanus and that eggs from sputum or other worm products from human faeces also belonged to this species. Based on these results, 26 of 168 persons (15.5%) were diagnosed as suffering from paragonimiasis...
  61. pmc Histopathological, serological, and molecular confirmation of indigenous alveolar echinococcosis cases in Mongolia
    Akira Ito
    Asahikawa Medical College, Midorigaoka Higashi 2 1 1 1, Asahikawa, Japan
    Am J Trop Med Hyg 82:266-9. 2010
    ..The most recent two cases were also confirmed serologically to be active alveolar echinococcosis...
  62. ncbi request reprint A phylogenetic hypothesis for the distribution of two genotypes of the pig tapeworm Taenia solium worldwide
    M Nakao
    Department of Parasitology, Asahikawa Medical College, Japan
    Parasitology 124:657-62. 2002
    ..solium was introduced recently into Latin America and Africa from different regions of Europe during the colonial age, which started 500 years ago, and that the tapeworm of another origin independently spread in Asian countries...
  63. pmc The first workshop towards the control of cestode zoonoses in Asia and Africa
    Akira Ito
    Asahikawa Medical University, Asahikawa, Japan
    Parasit Vectors 4:114. 2011
    ..hyaenae and other Taenia species from the wild animals in Ethiopia, and 5) how to detect echinococcosis patients and 6) how to differentiate Echinococcus species worldwide. Such important topics are summarized in this meeting report...
  64. pmc Usefulness of hydatid cyst fluid of Echinococcus granulosus developed in mice with secondary infection for serodiagnosis of cystic Echinococcosis in humans
    Wulamu Mamuti
    Department of Parasitology, Asahikawa Medical College, Asahikawa 078 8510, Japan
    Clin Diagn Lab Immunol 9:573-6. 2002
    ..HCF of E. granulosus, obtained from laboratory mice with a secondary infection with hydatid cyst tissue homogenates, appears to be highly useful for the serodiagnosis of CE in humans and may be useful in domestic animals...
  65. ncbi request reprint Short report: Evidence and potential for transmission of human and swine Taenia solium cysticercosis in the Piracuruca region, Piauí, Brazil
    Marcello Otake Sato
    Laboratorio de Parasitologia, Escola de Medicina Veterinária e Zootecnia, Universidade Federal do Tocantins, Araguaína TO, Brazil
    Am J Trop Med Hyg 75:933-5. 2006
    ..This study revealed the occurrence of human and porcine cysticercosis in the Piracuruca region of Piauí State, middle-north of Brazil...
  66. ncbi request reprint Echinococcus shiquicus, a new species from the Qinghai-Tibet plateau region of China: discovery and epidemiological implications
    Ning Xiao
    Institute of Parasitic Diseases, Sichuan Center for Disease Control and Prevention, Chengdu 610041, Sichuan, China
    Parasitol Int 55:S233-6. 2006
    ..E. shiquicus has been found at other areas surveyed on the plateau; however, no infections in humans caused by E. shiquicus have been yet identified...
  67. ncbi request reprint Comparison of the antigenicity of protoscoleces and microvesicles of Echinococcus multilocularis prepared from rats
    A Ito
    Department of Parasitology, Asahikawa Medical College, Asahikawa 078 8510, Japan
    J Helminthol 75:355-8. 2001
    ..Therefore, for serodiagnosis of E. multilocularis, antigens should be carefully prepared from protoscoleces rather than microvesicles from the rat...
  68. ncbi request reprint Experimental infection of larval Echinococcus multilocularis in the rodent brain as a model for cerebral alveolar echinococcosis
    Y Sato
    Department of Parasitology, Asahikawa Medical College, Japan
    J Helminthol 72:59-64. 1998
    ..Jirds were also found to be infected with metacestodes in the cranial cavity, but neither unusual behaviour nor establishment of cysts inside the brain was observed in jirds during the course of infection...
  69. ncbi request reprint Neurocysticercosis and echinococcosis in Asia: recent advances in the establishment of highly reliable differential serodiagnosis for international collaboration
    A Ito
    Department of Parasitology, Asahikawa Medical College, Japan
    Southeast Asian J Trop Med Public Health 31:16-20. 2000
    ..It is stressed that international collaboration or cooperation work on the control of parasitic diseases is only successful based on the original scientific contribution of high standard...
  70. ncbi request reprint Neurocysticercosis case with a single cyst in the brain showing dramatic drop in specific antibody titers within 1 year after curative surgical resection
    A Ito
    Department of Parasitology, Asahikawa Medical College, Japan
    Parasitol Int 48:95-9. 1999
    ..It is, therefore, strongly suggested that this case had a single cysticercus in his whole body and the presence of a single cysticercus was sufficient to evoke antibody responses against it...
  71. pmc Molecular characterization and diagnostic value of Taenia solium low-molecular-weight antigen genes
    Y Sako
    Department of Parasitology, Asahikawa Medical College, Asahikawa, Japan
    J Clin Microbiol 38:4439-44. 2000
    ..No assays of serum samples from patients with other parasitic infections recognized the Ag1V1/Ag2 chimeric protein. The Ag1V1/Ag2 chimeric protein obtained in this study had a high value for differential immunodiagnosis...
  72. ncbi request reprint Morphological changes of larval Echinococcus multilocularis in mice treated with albendazole or mebendazole
    K Nakaya
    Animal Laboratory for Medical Research, Asahikawa Medical College, Hokkaido, Japan
    J Helminthol 72:349-54. 1998
    ..These results indicate that these drugs do not eliminate larval E. multilocularis in the long-term, but mebendazole has a higher suppressive effect on multivesiculation than albendazole...
  73. ncbi request reprint ELISA and immunoblot using purified glycoproteins for serodiagnosis of cysticercosis in pigs naturally infected with Taenia solium
    A Ito
    Department of Parasitology, Asahikawa Medical College, Japan
    J Helminthol 73:363-5. 1999
    ..All sera from pigs showed similar responses to those in human cysticercosis. Therefore, it is expected that both ELISA and immunoblots using GPs would be useful in differentiating infected pigs from uninfected ones...
  74. ncbi request reprint Dogs as alternative intermediate hosts of Taenia solium in Papua (Irian Jaya), Indonesia confirmed by highly specific ELISA and immunoblot using native and recombinant antigens and mitochondrial DNA analysis
    A Ito
    Department of Parasitology, Asahikawa Medical College, Asahikawa 078 8510, Japan
    J Helminthol 76:311-4. 2002
    ..solium previously confirmed from pigs and biopsies from local people from Irian Jaya. It is suggested that the life cycle of T. solium may be completed not only between humans and pigs but also between humans and dogs...
  75. ncbi request reprint Recent advances in basic and applied science for the control of taeniasis/cysticercosis in Asia
    A Ito
    Department of Parasitology, Asahikawa Medical College, Asahikawa, Japan
    Southeast Asian J Trop Med Public Health 33:79-82. 2002
    ..Some aspects of the pathological differences between the Asian and Africa-American types and the antigenic components of these two types are discussed...
  76. ncbi request reprint Molecular identification of human echinococcosis in the Altai region of Russia
    Sergey V Konyaev
    Institute of Systematics and Ecology of Animals SB RAS, Novosibirsk, Russia
    Parasitol Int 61:711-4. 2012
    ..All of the AE cases were confirmed to be caused by Asian type Echinococcus multilocularis, while CE cases were caused by Echinococcus granulosus sensu stricto (genotype G1) and Echinococcus canadensis (genotype G6)...
  77. ncbi request reprint NOD-scid mouse as an experimental animal model for cysticercosis
    A Ito
    Department of Parasitology, Asahikawa Medical College, Japan
    Southeast Asian J Trop Med Public Health 32:85-9. 2001
    ..The animal model is not only available for human Taenia spp but can also be applied to other taeniid cestodes of economic importance or in veterinary parasitology...
  78. doi request reprint Multiple papules and nodules on the face and neck caused by the larvae of an unknown nematode: A noncreeping type eruption
    Hiroyuki Sakai
    Department of Dermatology, Asahikawa City Hospital, Asahikawa, Hokkaido, Japan
    J Am Acad Dermatol 58:668-70. 2008
    ..The patient never had any creeping or migratory eruptions. Our case is very unusual and differs from previously reported cases of cutaneous larva migrans...
  79. pmc Species identification of human echinococcosis using histopathology and genotyping in northwestern China
    Tiaoying Li
    Sichuan Centers for Disease Control and Prevention, Chengdu, Sichuan Province, PR China
    Trans R Soc Trop Med Hyg 102:585-90. 2008
    ....
  80. ncbi request reprint Evaluation of three PCR assays for the identification of the sheep strain (genotype 1) of Echinococcus granulosus in canid feces and parasite tissues
    Belgees S Boufana
    Biomedical Sciences Research Institute, University of Salford, United Kingdom
    Am J Trop Med Hyg 78:777-83. 2008
    ..Improvement of PCR tests for Echinococcus species and subspecific variants should rely on the use of less-conserved genes and development of protocols that improve the quality and quantity of DNA extracted from feces...
  81. ncbi request reprint Magnetic resonance imaging and immunoblot analyses in rats with experimentally induced cerebral alveolar echinococcosis
    Taketoshi Asanuma
    Laboratory of Radiation Biology, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060 0818, Japan
    Comp Med 53:649-56. 2003
    ..multilocularis. Using this secondary cerebral AE animal model, it was concluded that the MRI method was suitable for early detection of secondary cerebral AE...
  82. ncbi request reprint Taeniasis in Mongolia, 2002-2006
    Narankhajid Myadagsuren
    Department of Medical Biology, School of Biomedicine, Health Sciences University, Choidoglin Str 3, Ulaanbaatar, Mongolia
    Am J Trop Med Hyg 77:342-6. 2007
    ..saginata isolates from Mongolia. There was no evidence of Taenia solium taeniasis/cysticercosis or Taenia asiatica taeniasis. A diphyllobothriid segment was confirmed to be Diphyllobothrium latum by mitochondrial DNA analysis...
  83. ncbi request reprint Cysticercosis: IgG-ELISA evaluations of peak1 antigen and <30 kDa antigen of delipidized extract of Taenia solium metacestodes
    Paron Dekumyoy
    Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    Southeast Asian J Trop Med Public Health 35:1-9. 2004
    ..MWCOP1Ag gave higher sensitivity than that of DLPP1Ag but some cross-reactivity occurred...
  84. ncbi request reprint Short report: Echinococcus multilocularis confirmed on Kunashiri Island, 15 kilometers from the eastern part of Hokkaido, Japan
    Masao Satoh
    Department of Allergy and Immunology, Saitama Medical School, Saitama, Japan
    Am J Trop Med Hyg 72:284-8. 2005
    ..These results suggest that E. multilocularis may have been introduced to Hokkaido from Kunashiri Island during or after 1965...
  85. pmc Sympatric occurrence of Taenia solium, T. saginata, and T. asiatica, Thailand
    Malinee T Anantaphruti
    Mahidol University, Bangkok, Thailand
    Emerg Infect Dis 13:1413-6. 2007
    ..DNA analysis of morphologically identified T. saginata, in a dual infection with T. solium, indicated it was T. asiatica. To our knowledge, this report is the first of T. asiatica and a dual Taenia infection from Thailand...
  86. ncbi request reprint Magnetic resonance imaging of alveolar echinococcosis experimentally induced in the rat lung
    Taketoshi Asanuma
    Laboratory of Radiation Biology, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060 0818, Japan
    J Vet Med Sci 68:15-20. 2006
    ..multilocularis provided evidence for AE infection in the early stage. T1WI in combination with Western blot analysis could possibility become definitive and early signs of hematogenous pulmonary AE infection...