Affiliation: Akita Prefectural University
Crofts N, Abe N, Oitome N, Matsushima R, Hayashi M, Tetlow I, et al
. Amylopectin biosynthetic enzymes from developing rice seed form enzymatically active protein complexes. J Exp Bot. 2015;66:4469-82 pubmed publisher
..Detailed analyses of these complexes will shed light on the mechanisms controlling the unique branch and cluster structure of amylopectin, and the physicochemical properties of starch. ..
Hayashi M, Crofts N, Oitome N, Fujita N. Analyses of starch biosynthetic protein complexes and starch properties from developing mutant rice seeds with minimal starch synthase activities. BMC Plant Biol. 2018;18:59 pubmed publisher
..In addition, ss1 L /ss2a L /ss3a maintained the ability to synthesize starch and accumulated less amylopectin and more amylose in starch. ..
Fujita N, Yoshida M, Asakura N, Ohdan T, Miyao A, Hirochika H, et al
. Function and characterization of starch synthase I using mutants in rice. Plant Physiol. 2006;140:1070-84 pubmed
..In summary, this study strongly suggested that amylopectin chains are synthesized by the coordinated actions of SSI, SSIIa, and SSIIIa isoforms. ..
Toyosawa Y, Kawagoe Y, Matsushima R, Crofts N, Ogawa M, Fukuda M, et al
. Deficiency of Starch Synthase IIIa and IVb Alters Starch Granule Morphology from Polyhedral to Spherical in Rice Endosperm. Plant Physiol. 2016;170:1255-70 pubmed publisher
..Therefore, we propose that SSIIIa and SSIVb have crucial roles in determining starch granule morphology and in maintaining the amyloplast envelope structure. We present a model of spherical starch granule production. ..
Itoh Y, Crofts N, Abe M, Hosaka Y, Fujita N. Characterization of the endosperm starch and the pleiotropic effects of biosynthetic enzymes on their properties in novel mutant rice lines with high resistant starch and amylose content. Plant Sci. 2017;258:52-60 pubmed publisher
..The relationship linking starch structure, RS content, and starch biosynthetic enzymes in the new mutant lines has also been discussed. ..
Fujita N, Yoshida M, Kondo T, Saito K, Utsumi Y, Tokunaga T, et al
. Characterization of SSIIIa-deficient mutants of rice: the function of SSIIIa and pleiotropic effects by SSIIIa deficiency in the rice endosperm. Plant Physiol. 2007;144:2009-23 pubmed
Fujita N, Satoh R, Hayashi A, Kodama M, Itoh R, Aihara S, et al
. Starch biosynthesis in rice endosperm requires the presence of either starch synthase I or IIIa. J Exp Bot. 2011;62:4819-31 pubmed publisher
..This present study strongly suggests that at least SSI or SSIIIa is required for starch biosynthesis in rice endosperm...
Crofts N, Sugimoto K, Oitome N, Nakamura Y, Fujita N. Differences in specificity and compensatory functions among three major starch synthases determine the structure of amylopectin in rice endosperm. Plant Mol Biol. 2017;94:399-417 pubmed publisher
..These results revealed compensatory differences among three major SS isozymes responsible for lengths of amylopectin branches. ..
Crofts N, Nakamura Y, Fujita N. Critical and speculative review of the roles of multi-protein complexes in starch biosynthesis in cereals. Plant Sci. 2017;262:1-8 pubmed publisher
..Finally, we discuss the significance of protein complexes and describe new methods that may elucidate the mechanisms and roles of starch biosynthetic enzyme complexes. ..