Hiroyuki Nozaki

Summary

Affiliation: Ajinomoto Co
Country: Japan

Publications

  1. ncbi request reprint Production of D-arabitol by Metschnikowia reukaufii AJ14787
    Hiroyuki Nozaki
    AminoScience Laboratories, Ajinomoto Co, Ltd, Kawasaki, Japan
    Biosci Biotechnol Biochem 67:1923-9. 2003
  2. ncbi request reprint Gene cloning of alpha-methylserine aldolase from Variovorax paradoxus and purification and characterization of the recombinant enzyme
    Hiroyuki Nozaki
    AminoScience Laboratories, Ajinomoto Co, Inc, Kawasaki, Japan
    Biosci Biotechnol Biochem 72:2580-8. 2008
  3. pmc Purification and gene cloning of alpha-methylserine aldolase from Ralstonia sp. strain AJ110405 and application of the enzyme in the synthesis of alpha-methyl-L-serine
    Hiroyuki Nozaki
    AminoScience Laboratories, Ajinomoto Co, Inc, 1 1 Suzuki cho, Kawasaki ku, Kawasaki 210 8681, Japan
    Appl Environ Microbiol 74:7596-9. 2008
  4. ncbi request reprint Cloning of the gene encoding alpha-methylserine hydroxymethyltransferase from Aminobacter sp. AJ110403 and Ensifer sp. AJ110404 and characterization of the recombinant enzyme
    Hiroyuki Nozaki
    AminoScience Laboratories, Ajinomoto Co, Inc, Kawasaki, Japan
    Biosci Biotechnol Biochem 72:3002-5. 2008

Detail Information

Publications4

  1. ncbi request reprint Production of D-arabitol by Metschnikowia reukaufii AJ14787
    Hiroyuki Nozaki
    AminoScience Laboratories, Ajinomoto Co, Ltd, Kawasaki, Japan
    Biosci Biotechnol Biochem 67:1923-9. 2003
    ..Under optimal conditions, 206 g/l of D-arabitol was produced from D-glucose with a weight yield of 52% in 100 hours...
  2. ncbi request reprint Gene cloning of alpha-methylserine aldolase from Variovorax paradoxus and purification and characterization of the recombinant enzyme
    Hiroyuki Nozaki
    AminoScience Laboratories, Ajinomoto Co, Inc, Kawasaki, Japan
    Biosci Biotechnol Biochem 72:2580-8. 2008
    ..With L-alanine and L-2-aminobutyric acid as substrates, the specific peak, assumed to be a result of the formation of a quinonoid intermediate, increased at 498 nm and 500 nm respectively...
  3. pmc Purification and gene cloning of alpha-methylserine aldolase from Ralstonia sp. strain AJ110405 and application of the enzyme in the synthesis of alpha-methyl-L-serine
    Hiroyuki Nozaki
    AminoScience Laboratories, Ajinomoto Co, Inc, 1 1 Suzuki cho, Kawasaki ku, Kawasaki 210 8681, Japan
    Appl Environ Microbiol 74:7596-9. 2008
    ..We used cells of E. coli as a whole-cell catalyst to express the gene encoding alpha-methylserine aldolase and effectively obtained a high yield of optically pure alpha-methyl-L-serine using L-alanine and formaldehyde...
  4. ncbi request reprint Cloning of the gene encoding alpha-methylserine hydroxymethyltransferase from Aminobacter sp. AJ110403 and Ensifer sp. AJ110404 and characterization of the recombinant enzyme
    Hiroyuki Nozaki
    AminoScience Laboratories, Ajinomoto Co, Inc, Kawasaki, Japan
    Biosci Biotechnol Biochem 72:3002-5. 2008
    ..The V(max) of these enzymes catalyzing the conversion of alpha-methyl-L-serine to D-alanine via tetrahydrofolate was 22.1 U/mg (AJ110403) and 15.4 U/mg (AJ110404)...