Research Topics
Genomes and Genes
| Masaki InagakiSummaryAffiliation: Aichi Cancer Center Country: Japan Publications
| Collaborators
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Detail Information
Publications
Identification of trichoplein, a novel keratin filament-binding proteinMiwako Nishizawa
Division of Biochemistry, Aichi Cancer Center Research Institute, 1 1 Kanokoden, Chikusa ku, Nagoya 464 8681, Japan
J Cell Sci 118:1081-90. 2005..Taken together, these results suggest that trichoplein is a keratin-binding protein that may be involved in the organization of the apical network of keratin filaments and desmosomes in simple epithelial cells...
Nuclear Chk1 prevents premature mitotic entryMakoto Matsuyama
Division of Biochemistry, Aichi Cancer Center Research Institute, Chikusa ku, Nagoya 464 8681, Japan
J Cell Sci 124:2113-9. 2011..These results suggest that nuclear but not centrosomal Chk1 contributes to correct timing of mitotic entry...- Autophosphorylation of a newly identified site of Aurora-B is indispensable for cytokinesisYoshihiro Yasui
Division of Biochemistry, Aichi Cancer Center Research Institute, Chikusa ku, Nagoya, Aichi 464 8681, Japan
J Biol Chem 279:12997-3003. 2004..These results indicate that the phosphorylation of Thr-232 is an essential regulatory mechanism for Aurora-B activation... - Complex formation of Plk1 and INCENP required for metaphase-anaphase transitionHidemasa Goto
Division of Biochemistry, Aichi Cancer Center Research Institute, Nagoya, Aichi 464 8681, Japan
Nat Cell Biol 8:180-7. 2006..We propose that INCENP phosphorylation by Cdk1 is necessary for the recruitment of Plk1 to the kinetochore, and that the complex formation of Plk1 and Aurora-B on INCENP may play crucial roles in the regulation of chromosomal dynamics... 
The keratin-binding protein Albatross regulates polarization of epithelial cellsMasahiko Sugimoto
Division of Biochemistry, Aichi Cancer Center Research Institute, Chikusa ku, Nagoya 464 8681, Japan
J Cell Biol 183:19-28. 2008..We propose that keratins and the keratin-binding protein Albatross are important for epithelial cell polarization...- Novel positive feedback loop between Cdk1 and Chk1 in the nucleus during G2/M transitionMasato Enomoto
Division of Biochemistry, Aichi Cancer Center Research Institute, Nagoya, Aichi 464 8681, Japan
J Biol Chem 284:34223-30. 2009..These results indicate that Chk1 phosphorylation by Cdk1 participates in cytoplasmic sequestration of Chk1 activity, which releases Cdk1 inhibition in the nucleus and promotes mitotic entry... - P90 RSK arranges Chk1 in the nucleus for monitoring of genomic integrity during cell proliferationPing Li
Division of Biochemistry, Aichi Cancer Center Research Institute, 1 1 Kanokoden, Chikusa ku, Nagoya, Aichi 464 8681, Japan
Mol Biol Cell 23:1582-92. 2012..These results suggest that p90 RSK facilitates nuclear Chk1 accumulation through Chk1-Ser-280 phosphorylation and that this pathway plays an important role in the preparation for monitoring genetic stability during cell proliferation... - Regulation of mitotic function of Chk1 through phosphorylation at novel sites by cyclin-dependent kinase 1 (Cdk1)Takashi Shiromizu
Division of Biochemistry, Aichi Cancer Center Research Institute, Nagoya, Aichi 464 8681, Japan
Genes Cells 11:477-85. 2006..These observations imply the regulation of mitotic Chk1 function through Chk1 phosphorylation at novel sites by Cdk1... - Functional significance of the specific sites phosphorylated in desmin at cleavage furrow: Aurora-B may phosphorylate and regulate type III intermediate filaments during cytokinesis coordinatedly with Rho-kinaseAie Kawajiri
Division of Biochemistry, Aichi Cancer Center Research Institute, Chikusa ku, Nagoya 464 8681, Japan
Mol Biol Cell 14:1489-500. 2003..The results presented here suggest the possibility that Aurora-B may regulate cleavage furrow-specific phosphorylation and segregation of type III IFs coordinatedly with Rho-kinase during cytokinesis... - Filament formation of MSF-A, a mammalian septin, in human mammary epithelial cells depends on interactions with microtubulesKoh ichi Nagata
Divisions of Biochemistry and Virology, Aichi Cancer Center Research Institute, 1 1 Kanokoden, Chikusa ku, Nagoya 464 8681, Japan
J Biol Chem 278:18538-43. 2003..These results strongly suggest that septin filaments may interact not only with actin filaments but also with microtubule networks and that GTPase activity of MSF-A is not indispensable to incorporation of MSF-A into septin filaments... - 14-3-3gamma mediates Cdc25A proteolysis to block premature mitotic entry after DNA damageKousuke Kasahara
Division of Biochemistry, Aichi Cancer Center Research Institute, Nagoya, Aichi, Japan
EMBO J 29:2802-12. 2010..This ternary complex formation has an essential function in Cdc25A phosphorylation and degradation to block premature mitotic entry after DNA damage... - Trichoplein and Aurora A block aberrant primary cilia assembly in proliferating cellsAkihito Inoko
Division of Biochemistry, Aichi Cancer Center Research Institute, Chikusa ku, Nagoya, Japan
J Cell Biol 197:391-405. 2012..These data suggest that the trichoplein-AurA pathway is required for G1 progression through a key role in the continuous suppression of primary cilia assembly... - Phosphorylation by Cdk1 induces Plk1-mediated vimentin phosphorylation during mitosisTomoya Yamaguchi
Division of Biochemistry, Aichi Cancer Center Research Institute, Aichi 464 8681, Japan
J Cell Biol 171:431-6. 2005..Taken together, these results indicated a novel mechanism that Cdk1 regulated mitotic vimentin phosphorylation via not only a direct enzyme reaction but also Plk1 recruitment to vimentin... - Trichoplein controls microtubule anchoring at the centrosome by binding to Odf2 and nineinMiho Ibi
Division of Biochemistry, Aichi Cancer Center Research Institute, Chikusa ku, Nagoya 464 8681, Japan
J Cell Sci 124:857-64. 2011..These results suggest that trichoplein has a crucial role in MT-anchoring activity at the centrosome in proliferating cells, probably through its complex formation with Odf2 and ninein... - Densin-180, a synaptic protein, links to PSD-95 through its direct interaction with MAGUIN-1Kazuhiro Ohtakara
Division of Biochemistry, Aichi Cancer Center Research Institute, Chikusa ku, Nagoya, Aichi 464 8681, Japan
Genes Cells 7:1149-60. 2002.... - Aurora-B and Rho-kinase/ROCK, the two cleavage furrow kinases, independently regulate the progression of cytokinesis: possible existence of a novel cleavage furrow kinase phosphorylates ezrin/radixin/moesin (ERM)Tomoya Yokoyama
Division of Biochemistry, Aichi Cancer Center Research Institute, Chikusa-ku, Nagoya, Aichi 464-8681, Japan
Genes Cells 10:127-37. 2005..These results suggest that Aurora-B and Rho-kinase/ROCK regulate the progression of cytokinesis without communicating to each other, and there may exist a novel protein kinase which phosphorylates ERM at the cleavage furrow... - Chk1 phosphorylation at Ser286 and Ser301 occurs with both stalled DNA replication and damage checkpoint stimulationYosuke Ikegami
Division of Biochemistry, Aichi Cancer Center Research Institute, Nagoya, Aichi 464 8681, Japan
Biochem Biophys Res Commun 377:1227-31. 2008..Therefore, Chk1 phosphorylation in the checkpoint response is regulated not only by ATR but also by Cdk2... - Aurora-B regulates the cleavage furrow-specific vimentin phosphorylation in the cytokinetic processHidemasa Goto
Division of Biochemistry, Aichi Cancer Center Research Institute, Chikusa ku, Nagoya, Japan
J Biol Chem 278:8526-30. 2003..Our findings indicate that Aurora-B regulates the cleavage furrow-specific vimentin phosphorylation and controls vimentin filament segregation in cytokinetic process... - Palmitoylation of ERBIN is required for its plasma membrane localizationIchiro Izawa
Division of Biochemistry, Aichi Cancer Center Research Institute, Chikusa ku, Nagoya, Aichi 464 8687, Japan
Genes Cells 13:691-701. 2008..These results suggest that both palmitoylation and LRR are required for the plasma membrane localization of ERBIN... - Densin-180 interacts with delta-catenin/neural plakophilin-related armadillo repeat protein at synapsesIchiro Izawa
Division of Biochemistry, Aichi Cancer Center Research Institute, Chikusa ku, Nagoya, Aichi 464 8681, Japan
J Biol Chem 277:5345-50. 2002..Densin-180 co-localized with delta-catenin/NPRAP at synapses in delta-catenin/NPRAP and may be involved in organization of the synaptic cell-cell junction through interaction with the delta-catenin/NPRAP-N-cadherin complex... - Vimentin-Ser82 as a memory phosphorylation site in astrocytesTakashi Oguri
Division of Biochemistry, Aichi Cancer Center Research Institute, Nagoya 464 8681, Japan
Genes Cells 11:531-40. 2006..Taken together, these results suggest that vimentin is a novel member of binding partner of PP1c in astrocytes, and vimentin-Ser82 may act as a memory phosphorylation site... - Biochemical and cell biological characterization of a mammalian septin, Sept11Nobuhiro Hanai
Division of Biochemistry, Aichi Cancer Center Research Institute, 1 1 Kanokoden, Chikusa ku, Nagoya 464 8681, Japan
FEBS Lett 568:83-8. 2004..Sept11 partially colocalized with stress fibers and microtubules in HeLa cells... - Approaches to study phosphorylation of intermediate filament proteins using site-specific and phosphorylation state-specific antibodiesAie Kawajiri
Division of Biochemistry, Aichi Cancer Center Research Institute, Nagoya, Aichi 464-8681, Japan
Methods Cell Biol 78:353-71. 2004 - Cytoskeletal modification of Rho guanine nucleotide exchange factor activity: identification of a Rho guanine nucleotide exchange factor as a binding partner for Sept9b, a mammalian septinKoh ichi Nagata
Division of Biochemistry, Aichi Cancer Center Research Institute, 1 1 Kanokoden, Chikusa ku, Nagoya 464 8681, Japan
Oncogene 24:65-76. 2005..SA-RhoGEF and its fragments expressed in REF52 cells altered endogenous septin filament structures. To our knowledge, SA-RhoGEF is the first molecule providing a link between septins and Rho signaling... - Phosphorylation and reorganization of vimentin by p21-activated kinase (PAK)Hidemasa Goto
Division of Biochemistry, Aichi Cancer Center Research Institute, Chikusa ku, Nagoya 464 8681, Japan
Genes Cells 7:91-7. 2002..Vimentin is the most widely expressed IF protein component. The Rho family of small GTPases, such as Cdc42, Rac and Rho, are thought to control the organization of actin filaments as well as other cytoskeletal filaments... - ERBIN associates with p0071, an armadillo protein, at cell-cell junctions of epithelial cellsIchiro Izawa
Division of Biochemistry, Aichi Cancer Center Research Institute, Chikusa ku, Nagoya, Aichi 464 8681, Japan
Genes Cells 7:475-85. 2002..ERBIN, an ErbB2 receptor-interacting protein, belongs to a recently described family of proteins termed the LAP [leucine-rich repeats and PSD-95/dLg-A/ZO-1 (PDZ) domains] family which has essential roles in establishment of cell polarity... - Regulatory mechanisms and functions of intermediate filaments: a study using site- and phosphorylation state-specific antibodiesIchiro Izawa
Division of Biochemistry, Aichi Cancer Center Research Institute, Nagoya 464-8681, Japan
Cancer Sci 97:167-74. 2006..Elucidation of the precise molecular functions of IF is expected to improve our understanding of tumor development, invasion and metastasis... - Characterization of a monoclonal antibody, HTA28, recognizing a histone H3 phosphorylation site as a useful marker of M-phase cellsAkihiro Hirata
Division of Oncological Pathology, Aichi Cancer Center Research Institute, 1-1 Kanokoden, Chikusa-ku, Nagoya 464-8681, Japan
J Histochem Cytochem 52:1503-9. 2004..These therefore provide strong evidence for the applicability of HTA28 as an M-phase marker. We also showed that antigenicity for HTA28 is lost if tissue is not immediately fixed after sampling... - [Development of site- and phosphorylation state-specific antibodies]Ichiro Izawa
Tanpakushitsu Kakusan Koso 50:1555-62. 2005 - Novel regulation of checkpoint kinase 1: Is checkpoint kinase 1 a good candidate for anti-cancer therapy?Hidemasa Goto
Division of Biochemistry, Aichi Cancer left Research Institute, Nagoya, Japan
Cancer Sci 103:1195-200. 2012..In this review, we summarize recent advances in Chk1 regulation by phosphorylation, and discuss Chk1 as a molecular target for cancer therapeutics... - Aurora-B phosphorylates Histone H3 at serine28 with regard to the mitotic chromosome condensationHidemasa Goto
Division of Biochemistry, Aichi Cancer Center Research Institute, Chikusa ku, Nagoya, Aichi464 8681, Japan
Genes Cells 7:11-7. 2002..Aurora B was recently demonstrated to be responsible for Ser10 phosphorylation in S. cerevisiae, C. elegans, Drosophila and Xenopus egg extract... - Production of a site- and phosphorylation state-specific antibodyHidemasa Goto
Division of Biochemistry, Aichi Cancer Center Research Institute, Nagoya, Aichi 464 8681, Japan
Nat Protoc 2:2574-81. 2007..This protocol is also applicable to the production of other types of antibodies, which specifically recognize the site-specific modification, such as acetylation, methylation and proteolysis. The protocol can be completed in 2-3 months...