David E James
Yang G, Humphrey S, Murashige D, Francis D, Wang Q, Cooke K, et al
. RagC phosphorylation autoregulates mTOR complex 1. EMBO J. 2018;: pubmed publisher
..Finally, we identify mTORC1 as the upstream kinase of RagC on S21. Our data highlight the importance of RagC phosphorylation in its function and identify a previously unappreciated auto-regulatory mechanism of mTORC1 activity. ..
Fazakerley D, Krycer J, Kearney A, Hocking S, James D. Muscle and adipose tissue insulin resistance: malady without mechanism?. J Lipid Res. 2018;: pubmed publisher
..This review highlights key points in this pathway that require further research effort. ..
Tan S, Fisher Wellman K, Fazakerley D, Ng Y, Pant H, Li J, et al
. Selective insulin resistance in adipocytes. J Biol Chem. 2015;290:11337-48 pubmed publisher
..We conclude that insulin resistance in adipose tissue is highly selective for glucose metabolism and likely involves a defect in one of the components regulating GLUT4 translocation to the cell surface in response to insulin. ..
Parker B, Yang G, Humphrey S, Chaudhuri R, Ma X, Peterman S, et al
. Targeted phosphoproteomics of insulin signaling using data-independent acquisition mass spectrometry. Sci Signal. 2015;8:rs6 pubmed publisher
Phua Y, Nguyen A, Roden D, Elsworth B, Deng N, Nikolic I, et al
. MicroRNA profiling of the pubertal mouse mammary gland identifies miR-184 as a candidate breast tumour suppressor gene. Breast Cancer Res. 2015;17:83 pubmed publisher
..These studies elucidate a new layer of regulation in the PI3K/AKT/mTOR pathway with relevance to mammary development and tumour progression and identify miR-184 as a putative breast tumour suppressor. ..
Su Z, Deshpande V, James D, Stöckli J. Tankyrase modulates insulin sensitivity in skeletal muscle cells by regulating the stability of GLUT4 vesicle proteins. J Biol Chem. 2018;293:8578-8587 pubmed publisher
..These studies reveal an important role for tankyrase in maintaining the stability of key GLUT4 regulatory proteins that in turn plays a role in regulating cellular insulin sensitivity. ..
Fazakerley D, Minard A, Krycer J, Thomas K, Stöckli J, Harney D, et al
. Mitochondrial oxidative stress causes insulin resistance without disrupting oxidative phosphorylation. J Biol Chem. 2018;293:7315-7328 pubmed publisher
Burchfield J, Kebede M, Meoli C, Stöckli J, Whitworth P, Wright A, et al
. High dietary fat and sucrose results in an extensive and time-dependent deterioration in health of multiple physiological systems in mice. J Biol Chem. 2018;293:5731-5745 pubmed publisher
..Overall, the excessive calorie intake was accompanied by deteriorating function of numerous physiological systems. ..
Parker B, Thaysen Andersen M, Fazakerley D, Holliday M, Packer N, James D. Terminal Galactosylation and Sialylation Switching on Membrane Glycoproteins upon TNF-Alpha-Induced Insulin Resistance in Adipocytes. Mol Cell Proteomics. 2016;15:141-53 pubmed publisher
..This revealed the modulation of N-glycosylation on specific proteins in IR, including those previously associated with insulin-stimulated GLUT4 trafficking to the plasma membrane. ..