Application of enzyme amplified mycobacterial DNA detection in the diagnosis of pulmonary & extra-pulmonary tuberculosisVandana Tiwari
Department of Microbiology, Chhatrapati Sahuji Maharaj Medical University (Upgraded KG. Medical College, Lucknow, India
Indian J Med Res 118:224-8. 2003
..INTERPRETATION & CONCLUSION: Routine application of DNA amplification method in diagnosis of AFB negative patients with pulmonary or extra-pulmonary tuberculosis may be a useful tool for detection of M. tuberculosis...
- High ampicillin resistance in different biotypes and serotypes of Haemophilus influenzae colonizing the nasopharynx of healthy school-going Indian children
Amita Jain
Department of Microbiology, King George s Medical University, Lucknow, UP, 226003, India
J Med Microbiol 55:133-7. 2006
..9 % were type II and 16.6 % were type III. Most of the strains belonging to biotypes III-VIII were ampicillin sensitive. Ampicillin resistance is significantly more common in biotype I and serotype b than in other biotypes and serotypes...
High nasopharyngeal carriage of beta-lactamase-negative ampicillin-resistant Haemophilus influenzae in north Indian school-going childrenAmita Jain
Department of Microbiology, King George s Medical University, Lucknow, U P India
J Infect Chemother 14:72-4. 2008
..BLNAR H. influenzae are no less pathogenic than beta-lactamase-positive H. influenzae. Higher level of drug resistance was found in beta-lactamase-producing H. influenzae in comparison to BLNAR isolates...
- Cefoxitin disc diffusion test for detection of meticillin-resistant staphylococci
Amita Jain
Department of Microbiology, King George s Medical University, Lucknow, UP, 226003, India
J Med Microbiol 57:957-61. 2008
..Centres that are not equipped to carry out PCR can use AD methods for confirmation of meticillin resistance, especially in oxacillin-resistant and cefoxitin-sensitive cases...
- Pre-XDR & XDR in MDR and Ofloxacin and Kanamycin resistance in non-MDR Mycobacterium tuberculosis isolates
Amita Jain
Department of Microbiology, C S M Medical University, Lucknow 226003, India
Tuberculosis (Edinb) 92:404-6. 2012
..3%) were resistant to either OFX or KM (Pre-XDR) & 11 (8.5%) were resistant to both KM & OFX (XDR). Resistance to quinolones & aminoglycosides should be routinely assessed in areas endemic for tuberculosis...
- Detection of extended spectrum beta-lactamase production in clinical isolates of Klebsiella spp
Amita Jain
Department of Microbiology, CSM Medical University, Lucknow, India
Indian J Med Res 127:344-6. 2008
..In this study, we report the screening sensitivity of four representative antimicrobial agents i.e., cefpodoxime, cefotaxime, ceftazidime and aztreonam, commonly used for ESBL detection in Klebsiella spp...
- Prevalence & antimicrobial resistance pattern of extended spectrum beta-lactamase producing Klebsiella spp isolated from cases of neonatal septicaemia
Amita Jain
Post Graduate Department of Microbiology, King George s Medical University, Lucknow, India
Indian J Med Res 125:89-94. 2007
..Here we report the prevalence and resistance pattern of ESBL producing Klebsiella spp isolated from cases of neonatal septicaemia at a tertiary care hospital from north India...
- Biofilm production, a marker of pathogenic potential of colonizing and commensal staphylococci
Amita Jain
Department of Microbiology, Chhatrapati Shahuji Maharaj Medical University, Lucknow, UP 226003, India
J Microbiol Methods 76:88-92. 2009
..63% and 90.79% for Staphylococcus aureus and 75.86% and 96.88% respectively for coagulase negative staphylococci. CRA is a method that could be used to determine whether an isolate has the potential for biofilm production or not...
- Antibiotic resistance pattern of group-a beta-hemolytic streptococci isolated from north Indian children
Amita Jain
Department of Microbiology, CSM Medical University, Lucknow, UP, India
Indian J Med Sci 62:392-6. 2008
..The current resistance pattern of GABHS (group-A beta-hemolytic streptococci) in India has not been discussed...
- Prevalence of multidrug resistant Mycobacterium tuberculosis in Lucknow, Uttar Pradesh
Amita Jain
Department of Microbiology, CSM Medical University, Lucknow, India
Indian J Med Res 128:300-6. 2008
..In the present study, we attempted to describe the current prevalence of MDR-TB in Lucknow district, Uttar Pradesh, and our observations on transmission of MDR isolates among populations in and around this area...
- High nasopharyngeal carriage of drug resistant Streptococcus pneumoniae and Haemophilus influenzae in North Indian schoolchildren
Amita Jain
Department of Microbiology, King George s Medical University, Lucknow, India
Trop Med Int Health 10:234-9. 2005
..Prevalence of resistance to various antimicrobial drugs among S. pneumoniae and H. influenzae was estimated...
- Dot-ELISA vs. PCR of fine needle aspirates of tuberculous lymphadenitis: a prospective study in India
Amita Jain
Department of Microbiology, K G Medical University, Lucknow, UP, India
Acta Cytol 49:17-21. 2005
..To evaluate an in-house dot-enzyme-linked immunosorbent assay (ELISA) for confirmation of clinically suspected cases of tuberculous lymphadenitis (TBLN)...
- Prevalence of methicillin-resistant, coagulase-negative staphylococci in neonatal intensive care units: findings from a tertiary care hospital in India
Amita Jain
Post Graduate Department of Microbiology, King George s Medical University, Lucknow 226003, India
J Med Microbiol 53:941-4. 2004
..Given the large number of methicillin-resistant CNS, inclusion of vancomycin in empiric therapy for neonates with late-onset septicaemia may be justified...
- Novel mutations in emb B gene of ethambutol resistant isolates of Mycobacterium tuberculosis: a preliminary report
Amita Jain
Department of Microbiology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, India
Indian J Med Res 128:634-9. 2008
..The present study was planned to investigate the presence of EMB resistance in M. tuberculosis isolates and to look for prevalent mutations in embB gene...
- Multidrug-resistant to extensively drug resistant tuberculosis: what is next?
Amita Jain
Post Graduate Department of Microbiology, CSM Medical University, Lucknow 226 003, India
J Biosci 33:605-16. 2008
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- TEM & SHV genes in extended spectrum beta-lactamase producing Klebsiella species beta their antimicrobial resistance pattern
Amita Jain
Department of Microbiology, C S M Medical University, Lucknow, India
Indian J Med Res 128:759-64. 2008
..We investigated the presence of TEM and SHV genes in ESBL producing Klebsiella spp. and their antimicrobial resistance pattern in cases of neonatal septicaemia in a tertiary care hospital...
- Prevalence of extended-spectrum beta-lactamase-producing Gram-negative bacteria in septicaemic neonates in a tertiary care hospital
Amita Jain
Departments of Microbiology, King George s Medical College, Lucknow, UP 226 003, India
J Med Microbiol 52:421-5. 2003
..It is concluded that indiscriminate use of third-generation cephalosporins may be responsible for the selection of ESBL-producing multiresistant strains in the neonatal intensive-care unit (NICU)...
- Inhibition of Propionibacterium acnes-induced mediators of inflammation by Indian herbs
A Jain
Department of Microbiology, King George s Medical College, Lucknow, India
Phytomedicine 10:34-8. 2003
..Thus, these herbs shows anti-inflammatory activity by suppressing the capacity of P. acnes-induced ROS and pro-inflammatory cytokines, the two important inflammatory mediators in acne pathogenesis...
- Development of a new antigen detection dot-ELISA for diagnosis of tubercular lymphadenitis in fine needle aspirates
Amita Jain
Department of Microbiology, K G Medical College, Lucknow 226003 U P, India
J Microbiol Methods 53:107-12. 2003
..5% of scanty aspirates. The assay was found to be 91.6% sensitive and 85.7% specific. The assay was found to be simple and rapid, and hence, could be performed in areas where health facilities are rudimentary...
- Extensively drug-resistant tuberculosis: current challenges and threats
Amita Jain
Department of Microbiology, C S M Medical University, Lucknow, India
FEMS Immunol Med Microbiol 53:145-50. 2008
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- Effect of green tea extract (catechins) in reducing oxidative stress seen in patients of pulmonary tuberculosis on DOTS Cat I regimen
Astha Agarwal
Department of Microbiology, Chhatrapati Shahuji Maharaj Medical University, Lucknow, UP 226003, India
Phytomedicine 17:23-7. 2010
..Hence, the present study was undertaken to assess the effect of crude green tea catechin in reducing the oxidative stress seen in patients of AFB positive pulmonary tuberculosis...
Intravenous device associated blood stream staphylococcal infection in paediatric patientsAmita Jain
Department of Microbiology, Chhatrapati Shahuji Maharaj Medical University, Lucknow, India
Indian J Med Res 134:193-9. 2011
..Prevalence of resistance to commonly used antimicrobials in hospital acquired staphylococcal isolates was also tested...
- Significance of coagulase negative staphylococci in neonates with late onset septicemia
Seema Bansal
Post Graduate Department of Microbiology, King George's Medical University, Lucknow
Indian J Pathol Microbiol 47:586-8. 2004
..Recovery of CONS from blood of a septicemic neonate needs to be viewed with caution since not all of them are true bacteremic agents...
- Evaluation of nitrate reduction assay, resazurin microtiter assay and microscopic observation drug susceptibility assay for first line antitubercular drug susceptibility testing of clinical isolates of M. tuberculosis
Pratima Dixit
Department of Microbiology, C S M Medical University, Lucknow 226003, India
J Microbiol Methods 88:122-6. 2012
..Optimal method for identifying drug-resistant M. tuberculosis in a timely and affordable way in resource-limited settings is not yet available...
- Atypical bacterial pathogens in community-acquired pneumonia in children: a hospital-based study
Jyotsna Agarwal
Chhatrapati Shahuji Maharaj Medical University, Lucknow, Uttar Pradesh, India
Trop Doct 39:109-11. 2009
..There was no significant association with any of the clinical, laboratory and radiological variables in children with pneumonia by the atypical pathogen...
- Nucleic acid amplification of Mycobacterium tuberculosis complex DNA from archival fine needle aspiration smear scrapings vs. fresh fine needle aspirates of tuberculous lymphadenitis
Madhu Mati Goel
Department of Pathology, Emergency Trauma Centre, King George s Medical University, Lucknow, U P, India
Acta Cytol 50:393-7. 2006
....
- Epidemiology and microbiology of nosocomial pediatric candidemia at a northern Indian tertiary care hospital
Avijit Kumar Awasthi
Department of Microbiology, Chhatrapati Shahuji Maharaj Medical University Erstwhile King George s Medical University, Lucknow, Uttar Pradesh, 226 003, India
Mycopathologia 172:269-77. 2011
..It was also noted that some strain types are circulating within the ward. High prevalence of non-albicans candidemia with high resistance to fluconazole is prevalent in North Indian hospitalized children...
- Longer duration of mechanical ventilation was found to be associated with ventilator-associated pneumonia in children aged 1 month to 12 years in India
Shally Awasthi
Department of Pediatrics, King George s Medical University, Shahmina Road, Lucknow 226003, Uttar Pradesh, India
J Clin Epidemiol 66:62-6. 2013
..To determine primarily (1) the incidence of ventilator-associated pneumonia (VAP) among ventilated patients aged 1 month to 12 years and secondarily (2) the risk factors for VAP and (3) common organisms causing VAP...
- Medical significance and management of staphylococcal biofilm
Astha Agarwal
Department of Microbiology, Chhatrapati Shahuji Maharaj Medical University, Lucknow, UP, India
FEMS Immunol Med Microbiol 58:147-60. 2010
..In this article, we reviewed the structure and formation of staphylococcal biofilm and its role in medical infections...
- Blood zinc levels in children hospitalized with severe pneumonia: a case control study
Saket Kumar
Department of Pediatrics, KG Medical University, Lucknow, Lucknow, India
Indian Pediatr 41:486-91. 2004
..Influenzae from nasopharyngeal swab. Cotrimoxazole resistant S. pneumoniae were isolated from 95% of cases and 41.2 % of controls (P = 0. 0004). Therefore, the role of zinc in treatment of severe pneumonia should be investigated...
- Trends in neonatal septicemia: emergence of non-albicans Candida
Jyotsna Agarwal
Department of Microbiology, King George's Medical University, Lucknow, India
Indian Pediatr 41:712-5. 2004
..2%) isolates of Candida were significant, suggesting that three in every four Candida isolated from blood can be significant. Non-albicans Candida are emerging as important pathogens for neonatal septicemia...
- Antibodies to mycobacterial antigens for diagnosis of tuberculosis
Raj Kumar Verma
Department of Microbiology, King George s Medical University, Lucknow, UP, India
FEMS Immunol Med Microbiol 51:453-61. 2007
..Each antigen is reviewed for its application in diagnosis, sensitivity, specificity, cross-reactivity and clinical application...
- Protective activity of picroliv on hepatic amoebiasis associated with carbon tetrachloride toxicity
Madhulika Singh
Division of Microbiology, Central Drug Research Institute, Lucknow, India
Indian J Med Res 121:676-82. 2005
..This study aims to evaluate the hepatoprotective action of picroliv against the hepatotoxic changes induced by carbon tetrachloride (CCl(4)) and E. histolytica infection in three animal models...
- Protective effect of green tea extract against the erythrocytic oxidative stress injury during mycobacterium tuberculosis infection in mice
R S Guleria
Department of Microbiology, K. G's. Medical College, Lucknow, UP, India
Mol Cell Biochem 236:173-81. 2002
..The findings thus, portray that there is a high oxidative stress during early stages of tuberculosis and antioxidants such as green tea extract, can play a vital role by reducing stress through adjuvant therapy...
- Development of modified diagnostic criteria for bacterial vaginosis at peripheral health centres in developing countries
Vineeta Mittal
Era s Lucknow Medical College and Hospital, Lucknow, India
J Infect Dev Ctries 6:373-7. 2012
..In clinical practice, BV is diagnosed using Amsel criteria and Nugent scoring. Using modified Amsel criteria, we determined that the fulfilling of any two instead of three criteria can be diagnostic of BV...
- Trend of dengue virus infection at Lucknow, north India (2008- 2010): a hospital based study
Nidhi Pandey
Department of Microbiology, King George s Medical University, Lucknow, India
Indian J Med Res 136:862-7. 2012
..Dengue virus (DV) infection has emerged as a major health problem in north India. Here, we report the annual trend of dengue virus infection as seen in Lucknow, Uttar Pradesh, during 2008-2010...
- 1H NMR spectroscopy in the diagnosis of Klebsiella pneumoniae-induced urinary tract infection
Ashish Gupta
Centre of Biomedical Magnetic Resonance, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Raebareli Road, Lucknow 226 014, India
NMR Biomed 19:1055-61. 2006
..In the present authors' experience, the results of NMR spectroscopy based screening show a very good correlation with the diagnosis of urinary tract infected patients...
- Extensively drug-resistant Mycobacterium tuberculosis, India
Rajesh Mondal
Emerg Infect Dis 13:1429-31. 2007
- Molecular typing of Mycobacterium tuberculosis isolates from different parts of India based on IS6110 element polymorphism using RFLP analysis
D S Chauhan
National JALMA Institute for Leprosy and Other Mycobacterial Diseases, Agra, India
Indian J Med Res 125:577-81. 2007
..The aim of the study was to characterize M. tuberculosis isolates obtained from different parts of India based on IS6110 element polymorphism using restriction fragment length polymorphism (RFLP) analysis...
- Small intestinal submucosa as a tunica albuginea graft material
Manoj Monga
Department of Urologic Surgery, University of Minnesota, Minneapolis, Minnesota, USA
J Urol 168:1215-21. 2002
..Stimulating VEGF may facilitate wound healing and the maintenance of normal erectile function...
