Alessandro Weisz

Summary

Affiliation: University of Salerno, Laboratory of Molecular Medicine and Genomics
Location: Baronissi (SA), Italy
URL: http://www3.unisa.it/docenti/weisz/index
Summary:
Research expertise:
- Molecular mechanisms of disease
- Functional investigations on cancer promotion and progression
- Regulation of gene transcription by nuclear receptors and extracellular stimuli
- Genomics of hormone-responsive breast cancer (http://www.hrbc-genomics.net/)
- Proteomics of signal transduction by hormones
- Computational genomics and bioinformatics
- Nwxt genration sequencing and microarray technologies
Grants:
Italian Ministry of Education, University and Research (FIRB and PRIN grants)
European Union (FP6 and FP7 IP grant and COST Action)
Regione Campania
Italian Association for Cancer Research
Foundation for Southern Italy
Foundation U. Veronesi

Publications

  1. doi Effects of oestrogen on microRNA expression in hormone-responsive breast cancer cells
    Lorenzo Ferraro
    Laboratory of Molecular Medicine and Genomics, Faculty of Medicine and Surgery, University of Salerno, via S Allende 1, 84081 Baronissi, Salerno, Italy
    Horm Cancer 3:65-78. 2012
  2. ncbi Molecular identification of ERalpha-positive breast cancer cells by the expression profile of an intrinsic set of estrogen regulated genes
    Alessandro Weisz
    Dipartimento di Patologia Generale, Seconda Universita degli Studi di Napoli, Vico L De Crecchio 7, Napoli, Italy
    J Cell Physiol 200:440-50. 2004
  3. pmc Estrogens and progesterone promote persistent CCND1 gene activation during G1 by inducing transcriptional derepression via c-Jun/c-Fos/estrogen receptor (progesterone receptor) complex assembly to a distal regulatory element and recruitment of cyclin D1 t
    Luigi Cicatiello
    Dipartimento di Patologia Generale, Seconda Universita degli Studi di Napoli, 80138 Naples, Italy
    Mol Cell Biol 24:7260-74. 2004
  4. ncbi Tumor-selective action of HDAC inhibitors involves TRAIL induction in acute myeloid leukemia cells
    Angela Nebbioso
    Dipartimento di Patologia Generale, Seconda Universita degli Studi di Napoli, Vico Luigi de Crecchio 7, 80138, Napoli, Italy
    Nat Med 11:77-84. 2005
  5. ncbi Comparative gene expression profiling reveals partially overlapping but distinct genomic actions of different antiestrogens in human breast cancer cells
    Claudio Scafoglio
    Dipartimento di Patologia Generale, Seconda Universita degli Studi di Napoli, Vico L De Crecchio 7, 80138 Naples, Italy
    J Cell Biochem 98:1163-84. 2006
  6. pmc Time-course analysis of genome-wide gene expression data from hormone-responsive human breast cancer cells
    Margherita Mutarelli
    Department of General Pathology Second University of Napoli, Napoli, Italy
    BMC Bioinformatics 9:S12. 2008
  7. pmc Estrogen receptor alpha controls a gene network in luminal-like breast cancer cells comprising multiple transcription factors and microRNAs
    Luigi Cicatiello
    Department of General Pathology, Second University of Naples, Napoli, Italy
    Am J Pathol 176:2113-30. 2010
  8. ncbi Epigenetic alteration of microRNAs in DNMT3B-mutated patients of ICF syndrome
    Sole Gatto
    Institute of Genetics and Biophysics A B T, CNR, Naples, Italy
    Epigenetics 5:427-43. 2010
  9. doi The p63 target HBP1 is required for skin differentiation and stratification
    S Borrelli
    Dipartimento di Scienze Biomolecolari e Biotecnologie, Universita degli Studi di Milano, Via Celoria 26, 20133 Milano, Italy
    Cell Death Differ 17:1896-907. 2010
  10. pmc C/EBPδ gene targets in human keratinocytes
    Serena Borrelli
    Dipartimento di Scienze Biomolecolari e Biotecnologie, Universita degli Studi di Milano, Milano, Italy
    PLoS ONE 5:e13789. 2010

Collaborators

Detail Information

Publications46

  1. doi Effects of oestrogen on microRNA expression in hormone-responsive breast cancer cells
    Lorenzo Ferraro
    Laboratory of Molecular Medicine and Genomics, Faculty of Medicine and Surgery, University of Salerno, via S Allende 1, 84081 Baronissi, Salerno, Italy
    Horm Cancer 3:65-78. 2012
    ....
  2. ncbi Molecular identification of ERalpha-positive breast cancer cells by the expression profile of an intrinsic set of estrogen regulated genes
    Alessandro Weisz
    Dipartimento di Patologia Generale, Seconda Universita degli Studi di Napoli, Vico L De Crecchio 7, Napoli, Italy
    J Cell Physiol 200:440-50. 2004
    ..These results provide direct evidence that the expression pattern of a limited number of estrogen responsive genes can be exploited to assess the estrogen signaling status of BC cells both in vitro and ex-vivo...
  3. pmc Estrogens and progesterone promote persistent CCND1 gene activation during G1 by inducing transcriptional derepression via c-Jun/c-Fos/estrogen receptor (progesterone receptor) complex assembly to a distal regulatory element and recruitment of cyclin D1 t
    Luigi Cicatiello
    Dipartimento di Patologia Generale, Seconda Universita degli Studi di Napoli, 80138 Naples, Italy
    Mol Cell Biol 24:7260-74. 2004
    ....
  4. ncbi Tumor-selective action of HDAC inhibitors involves TRAIL induction in acute myeloid leukemia cells
    Angela Nebbioso
    Dipartimento di Patologia Generale, Seconda Universita degli Studi di Napoli, Vico Luigi de Crecchio 7, 80138, Napoli, Italy
    Nat Med 11:77-84. 2005
    ..No apoptosis was seen in normal CD34(+) progenitor cells. Our results identify TRAIL as a mediator of the anticancer action of HDACIs...
  5. ncbi Comparative gene expression profiling reveals partially overlapping but distinct genomic actions of different antiestrogens in human breast cancer cells
    Claudio Scafoglio
    Dipartimento di Patologia Generale, Seconda Universita degli Studi di Napoli, Vico L De Crecchio 7, 80138 Naples, Italy
    J Cell Biochem 98:1163-84. 2006
    ....
  6. pmc Time-course analysis of genome-wide gene expression data from hormone-responsive human breast cancer cells
    Margherita Mutarelli
    Department of General Pathology Second University of Napoli, Napoli, Italy
    BMC Bioinformatics 9:S12. 2008
    ....
  7. pmc Estrogen receptor alpha controls a gene network in luminal-like breast cancer cells comprising multiple transcription factors and microRNAs
    Luigi Cicatiello
    Department of General Pathology, Second University of Naples, Napoli, Italy
    Am J Pathol 176:2113-30. 2010
    ....
  8. ncbi Epigenetic alteration of microRNAs in DNMT3B-mutated patients of ICF syndrome
    Sole Gatto
    Institute of Genetics and Biophysics A B T, CNR, Naples, Italy
    Epigenetics 5:427-43. 2010
    ..These results provide a better understanding of how DNA methylation and histone code interact to regulate the class of microRNA genes and enable us to predict molecular events possibly contributing to ICF condition...
  9. doi The p63 target HBP1 is required for skin differentiation and stratification
    S Borrelli
    Dipartimento di Scienze Biomolecolari e Biotecnologie, Universita degli Studi di Milano, Via Celoria 26, 20133 Milano, Italy
    Cell Death Differ 17:1896-907. 2010
    ....
  10. pmc C/EBPδ gene targets in human keratinocytes
    Serena Borrelli
    Dipartimento di Scienze Biomolecolari e Biotecnologie, Universita degli Studi di Milano, Milano, Italy
    PLoS ONE 5:e13789. 2010
    ..Our data indicate that C/EBPδ plays a role in late stages of keratinocyte differentiation...
  11. pmc The reverse transcription inhibitor abacavir shows anticancer activity in prostate cancer cell lines
    Francesca Carlini
    Department of Therapeutic Research and Drug Evaluation, Istituto Superiore di Sanita, Rome, Italy
    PLoS ONE 5:e14221. 2010
    ..In the present study we analyze the anticancer activity of Abacavir (ABC), a nucleoside reverse transcription inhibitor (NRTI), on PC3 and LNCaP prostate cancer cell lines...
  12. doi Comparative analysis of nuclear estrogen receptor alpha and beta interactomes in breast cancer cells
    Giovanni Nassa
    Department of General Pathology, Second University of Naples, Via L De Crecchio 7, 80138 Napoli, Italy
    Mol Biosyst 7:667-76. 2011
    ..Altogether, these data provide new insights on the protein-protein interaction networks controlled by ER-α and -β that mediate their ability to transduce estrogen signaling in breast cancer cells...
  13. doi A large set of estrogen receptor β-interacting proteins identified by tandem affinity purification in hormone-responsive human breast cancer cell nuclei
    Giovanni Nassa
    Department of General Pathology, Second University of Naples, Napoli, Italy
    Proteomics 11:159-65. 2011
    ....
  14. doi Identification of proteins associated with ligand-activated estrogen receptor α in human breast cancer cell nuclei by tandem affinity purification and nano LC-MS/MS
    Roberta Tarallo
    Department of General Pathology, Second University of Naples, Napoli, Italy
    Proteomics 11:172-9. 2011
    ..This led to the identification of 264 ER-associated proteins, whose functions highlight the hinge role of ER-α in the coordination of multiple hormone-regulated nuclear processes in BC cells...
  15. doi Expression of c-jun is not mandatory for mouse hepatocyte proliferation induced by two nuclear receptor ligands: TCPOBOP and T3
    Vera P Leoni
    Department of Toxicology, University of Cagliari, Italy
    J Hepatol 55:1069-78. 2011
    ..We investigated the role of c-jun in normal and preneoplastic hepatocyte proliferation induced by ligands of nuclear receptors, which cause liver hyperplasia in the absence of cell loss/death...
  16. pmc Specific inhibition of NF-Y subunits triggers different cell proliferation defects
    Paolo Benatti
    Dipartimento di Biologia, Universita di Modena e Reggio Emilia, Via Campi 213 D, 41125 Modena, Italy
    Nucleic Acids Res 39:5356-68. 2011
    ..Our data suggest a critical role of NF-Y expression, highlighting that the lack of the single subunits are differently perceived by the cells, which activate diverse cell cycle blocks and signaling pathways...
  17. doi Direct regulation of microRNA biogenesis and expression by estrogen receptor beta in hormone-responsive breast cancer
    O Paris
    Department of General Pathology, Second University of Naples, Napoli, Italy
    Oncogene 31:4196-206. 2012
    ....
  18. ncbi A genomic view of estrogen actions in human breast cancer cells by expression profiling of the hormone-responsive transcriptome
    Luigi Cicatiello
    Dipartimento di Patologia Generale, Seconda Universita degli Studi di Napoli, Vico L De Crecchio 7, 80138 Napoli, Italy
    J Mol Endocrinol 32:719-75. 2004
    ....
  19. ncbi Identification of genes involved in growth inhibition of breast cancer cells transduced with estrogen receptor
    Anne Licznar
    INSERM U540 Molecular and Cellular Endocrinology of Cancers, 60 rue de Navacelles, 34090 Montpellier, France
    FEBS Lett 553:445-50. 2003
    ..By using cDNA macroarrays, we identified a novel collection of genes regulated by liganded ERalpha potentially regulating cell cycle, apoptosis, cell signalling, stress response and DNA repair...
  20. pmc Global analysis of estrogen receptor beta binding to breast cancer cell genome reveals an extensive interplay with estrogen receptor alpha for target gene regulation
    Oli M V Grober
    Department of General Pathology, Second University of Naples, vico L, De Crecchio 7, 80138 Napoli, Italy
    BMC Genomics 12:36. 2011
    ..The identification of the genomic targets of ERβ in hormone-responsive BC cells is thus a critical step to elucidate the roles of this receptor in estrogen signaling and tumor cell biology...
  21. ncbi Hypoxia response element of the human vascular endothelial growth factor gene mediates transcriptional regulation by nitric oxide: control of hypoxia-inducible factor-1 activity by nitric oxide
    H Kimura
    Investigative Treatment Division, National Cancer Center Research Institute East, Kashiwa, Chiba, Japan
    Blood 95:189-97. 2000
    ..These results show that there is a primary role for NO in the control of VEGF synthesis and in cell adaptations to hypoxia. (Blood. 2000;95:189-197)..
  22. ncbi Insulin up-regulates vascular endothelial growth factor and stabilizes its messengers in endometrial adenocarcinoma cells
    L Bermont
    , , Contract Recherche INSERM 96.01, , France
    J Clin Endocrinol Metab 86:363-8. 2001
    ..Therefore, insulin could contribute to the increased risk of endometrial carcinoma due to its ability to induce VEGF expression and thus participate in the maintenance of an angiogenic phenotype...
  23. ncbi Estrogens do not modify MAP kinase-dependent nuclear signaling during stimulation of early G(1) progression in human breast cancer cells
    S Caristi
    Dipartimento di Patologia Generale, , 80138 Napoli, Italy
    Cancer Res 61:6360-6. 2001
    ..Furthermore, hormonal regulation of G(1) gene transcription can occur even without additional activation of the Mek-Erk1/2 pathway by estrogen receptors...
  24. pmc Distinct signaling pathways mediate stimulation of cell cycle progression and prevention of apoptotic cell death by estrogen in rat pituitary tumor PR1 cells
    Simona Caporali
    Dipartimento di Patologia Generale, Seconda Universita degli Studi di Napoli, 80138 Napoli, Italy
    Mol Biol Cell 14:5051-9. 2003
    ..These data indicate the coexistence of two distinguishable estrogen signaling pathways in PR1 cells, characterized by different functions and sensitivity to hormones and antihormones...
  25. pmc Distinct nongenomic signal transduction pathways controlled by 17beta-estradiol regulate DNA synthesis and cyclin D(1) gene transcription in HepG2 cells
    Maria Marino
    Dipartimento di Biologia, Universita Roma Tre, I 00146 Rome, Italy
    Mol Biol Cell 13:3720-9. 2002
    ....
  26. ncbi Cell type-specific induction of cyclin D and cyclin-dependent kinase inhibitor p27(kip1) expression by estrogen in rat endometrium
    Y H Zhuang
    Department of Cell Biology, Tampere University Medical School, 33014, Tampere, Finland
    J Steroid Biochem Mol Biol 78:193-9. 2001
    ....
  27. ncbi Effects of nitric oxide donors on vascular endothelial growth factor gene induction
    Hideo Kimura
    Division of Vascular Surgery, Department of Surgery, Graduate School of Medicine, University of Tokyo, Tokyo, Japan
    Biochem Biophys Res Commun 296:976-82. 2002
    ..SNAP, GSNO, and NOC18 induced the accumulation of HIF-1alpha protein, while others did not. These results suggest that SNAP, GSNO, and NOC compounds are suitable for pharmacological studies in HIF-1-mediated VEGF gene activation by NO...
  28. ncbi 17beta-estradiol inhibits forskolin-induced vascular endothelial growth factor promoter in MCF-7 breast adenocarcinoma cells
    L Bermont
    , , 240 route de Dole, 25000, , France
    J Steroid Biochem Mol Biol 78:343-9. 2001
    ..Therefore, in breast cancers, estrogens could partly inhibit the effect of ligand-activated G protein-coupled receptors on VEGF expression...
  29. ncbi Estrogen induces early and timed activation of cyclin-dependent kinases 4, 5, and 6 and increases cyclin messenger ribonucleic acid expression in rat uterus
    L Altucci
    Institute of General Pathology and Oncology, Faculty of Medicine and Surgery, Second University of Naples, Italy
    Endocrinology 138:978-84. 1997
    ....
  30. ncbi Malignant mammary cells acquire independence from extracellular context for regulation of estrogen receptor alpha
    Virginia Novaro
    Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, and Dipartimento di Patologia generale, Seconda Universita degli Studi di Napoli, Napoli, Italy
    Clin Cancer Res 10:402S-9S. 2004
    ..Evidence is presented to show the relevance of the cross-talk between different signaling pathways in the constitution of a functional tissue organization and how this integration may be disrupted in the malignant phenotype...
  31. ncbi Identification of hypoxia-inducible factor 1 ancillary sequence and its function in vascular endothelial growth factor gene induction by hypoxia and nitric oxide
    H Kimura
    Investigative Treatment Division, National Cancer Center Research Institute East, Kashiwa, Chiba, 277-8577 Japan
    J Biol Chem 276:2292-8. 2001
    ..A common structure of the HRE, which consists of the HBS and the HAS, is seen among several hypoxia-inducible genes, suggesting the presence of a novel mechanism mediated by the HAS for the regulation of these genes...
  32. pmc Identification of a hormone-regulated dynamic nuclear actin network associated with estrogen receptor alpha in human breast cancer cell nuclei
    Concetta Ambrosino
    Department of General Pathology, Second University of Naples, 80138 Naples, Italy
    Mol Cell Proteomics 9:1352-67. 2010
    ....
  33. ncbi Inhibition of human breast cancer cell growth by blockade of the mevalonate-protein prenylation pathway is not prevented by overexpression of cyclin D1
    D Germano
    Istituto di Patologia Generale e Oncologia, , , Italy
    Breast Cancer Res Treat 67:23-33. 2001
    ....
  34. doi Pharmacogenomics and analogues of the antitumour agent N6-isopentenyladenosine
    Francesca Colombo
    Department of Experimental Oncology and Laboratories, Fondazione IRCCS Istituto Nazionale Tumori, Milan, Italy
    Int J Cancer 124:2179-85. 2009
    ..Thus, although i(6)A may inhibit cell proliferation by regulating the cell cycle, further studies are needed to identify active analogues potentially useful in vivo...
  35. doi Identification of new genes associated with breast cancer progression by gene expression analysis of predefined sets of neoplastic tissues
    Daniela Cimino
    Institute for Cancer Research and Treatment, Unit of Gynecological Oncology, University of Turin Medical School, Candiolo, Turin, Italy
    Int J Cancer 123:1327-38. 2008
    ..This work provides a novel set of genes that could be used as independent prognostic markers and potential drug targets for breast cancer...
  36. doi Quantitative expression profiling of highly degraded RNA from formalin-fixed, paraffin-embedded breast tumor biopsies by oligonucleotide microarrays
    Maria Ravo
    Dipartimento di Patologia generale and Centro Grandi Apparecchiature, Seconda Universita degli Studi di Napoli, Napoli, Italy
    Lab Invest 88:430-40. 2008
    ..The method proved itself sensitive, reproducible and accurate, when compared to results obtained by microarray analysis of RNA extracted from snap-frozen tissue of the same tumor...
  37. pmc iMir: An integrated pipeline for high-throughput analysis of small non-coding RNA data obtained by smallRNA-Seq
    Giorgio Giurato
    Laboratory of Molecular Medicine and Genomics, Department of Medicine and Surgery, University of Salerno, via Allende, 1, Salerno, Baronissi, Italy
    BMC Bioinformatics 14:362. 2013
    ..As a consequence, the analytical workflow is slowed down by the need for continuous interventions by the operator, a critical factor when large numbers of datasets need to be analyzed at once...