Vincenzo Zara

Summary

Affiliation: University of Lecce
Country: Italy

Publications

  1. ncbi request reprint Biogenesis of rat mitochondrial citrate carrier (CIC): the N-terminal presequence facilitates the solubility of the preprotein but does not act as a targeting signal
    Vincenzo Zara
    Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Universita di Lecce, Via provinciale Lecce Monteroni, Italy
    J Mol Biol 325:399-408. 2003
  2. ncbi request reprint Conjugated linoleic acid and hepatic lipogenesis in mouse: role of the mitochondrial citrate carrier
    Alessandra Ferramosca
    Department of Sciences and Biological and Environmental Technologies, University of Lecce, 73100 Lecce, Italy
    J Lipid Res 47:1994-2003. 2006
  3. doi request reprint Biogenesis of the yeast cytochrome bc1 complex
    Vincenzo Zara
    Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Universita del Salento, via prov le Lecce Monteroni, I 73100 Lecce, Italy
    Biochim Biophys Acta 1793:89-96. 2009
  4. doi request reprint Mitochondrial carrier protein biogenesis: role of the chaperones Hsc70 and Hsp90
    Vincenzo Zara
    Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Universita del Salento, Via provinciale Lecce Monteroni, I 73100 Lecce, Italy
    Biochem J 419:369-75. 2009
  5. ncbi request reprint Biogenesis of yeast dicarboxylate carrier: the carrier signature facilitates translocation across the mitochondrial outer membrane
    Vincenzo Zara
    Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Universita del Salento, Via provinciale Lecce Monteroni, I 73100 Lecce, Italy
    J Cell Sci 120:4099-106. 2007
  6. pmc Evidence that the assembly of the yeast cytochrome bc1 complex involves the formation of a large core structure in the inner mitochondrial membrane
    Vincenzo Zara
    Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Universita del Salento, Lecce, Italy
    FEBS J 276:1900-14. 2009
  7. ncbi request reprint Biogenesis of eel liver citrate carrier (CIC): negative charges can substitute for positive charges in the presequence
    Vincenzo Zara
    Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Universita di Lecce, Via provinciale Lecce Monteroni, I 73100 Lecce, Italy
    J Mol Biol 365:958-67. 2007
  8. ncbi request reprint Import of rat mitochondrial citrate carrier (CIC) at increasing salt concentrations promotes presequence binding to import receptor Tom20 and inhibits membrane translocation
    Vincenzo Zara
    Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Universita di Lecce, I 73100 Lecce, Italy
    J Cell Sci 118:3985-95. 2005
  9. ncbi request reprint Further insights into the assembly of the yeast cytochrome bc1 complex based on analysis of single and double deletion mutants lacking supernumerary subunits and cytochrome b
    Vincenzo Zara
    Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Universita di Lecce, Italy
    Eur J Biochem 271:1209-18. 2004
  10. doi request reprint Bcs1p can rescue a large and productive cytochrome bc(1) complex assembly intermediate in the inner membrane of yeast mitochondria
    Laura Conte
    Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Universita del Salento, Lecce, Italy
    Biochim Biophys Acta 1813:91-101. 2011

Collaborators

Detail Information

Publications25

  1. ncbi request reprint Biogenesis of rat mitochondrial citrate carrier (CIC): the N-terminal presequence facilitates the solubility of the preprotein but does not act as a targeting signal
    Vincenzo Zara
    Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Universita di Lecce, Via provinciale Lecce Monteroni, Italy
    J Mol Biol 325:399-408. 2003
    ..We conclude that mitochondrial presequences do not necessarily act as mediators of targeting. In the case of the CIC, the presequence appears to determine the folding state of the preprotein...
  2. ncbi request reprint Conjugated linoleic acid and hepatic lipogenesis in mouse: role of the mitochondrial citrate carrier
    Alessandra Ferramosca
    Department of Sciences and Biological and Environmental Technologies, University of Lecce, 73100 Lecce, Italy
    J Lipid Res 47:1994-2003. 2006
    ..These data indicate that the CIC may play a role in the onset of hepatic steatosis in CLA-fed mice by supplying the carbon source for de novo fatty acid synthesis...
  3. doi request reprint Biogenesis of the yeast cytochrome bc1 complex
    Vincenzo Zara
    Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Universita del Salento, via prov le Lecce Monteroni, I 73100 Lecce, Italy
    Biochim Biophys Acta 1793:89-96. 2009
    ..The identification and characterization of all these sub-complexes may help in defining the steps and the molecular events leading to bc1 assembly in yeast mitochondria...
  4. doi request reprint Mitochondrial carrier protein biogenesis: role of the chaperones Hsc70 and Hsp90
    Vincenzo Zara
    Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Universita del Salento, Via provinciale Lecce Monteroni, I 73100 Lecce, Italy
    Biochem J 419:369-75. 2009
    ..We propose that carrier presequences evolved to improve targeting competence by different mechanisms, depending on physical properties of the precursors in the cytosolic targeting environment...
  5. ncbi request reprint Biogenesis of yeast dicarboxylate carrier: the carrier signature facilitates translocation across the mitochondrial outer membrane
    Vincenzo Zara
    Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Universita del Salento, Via provinciale Lecce Monteroni, I 73100 Lecce, Italy
    J Cell Sci 120:4099-106. 2007
    ..We conclude that the carrier signature of the dicarboxylate carrier is involved both in the biogenesis and in the transport activity of the functional protein...
  6. pmc Evidence that the assembly of the yeast cytochrome bc1 complex involves the formation of a large core structure in the inner mitochondrial membrane
    Vincenzo Zara
    Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Universita del Salento, Lecce, Italy
    FEBS J 276:1900-14. 2009
    ..The characterization of this novel core structure of the bc(1) complex provides a number of new elements clarifying the molecular events leading to the maturation of the yeast cytochrome bc(1) complex in the inner mitochondrial membrane...
  7. ncbi request reprint Biogenesis of eel liver citrate carrier (CIC): negative charges can substitute for positive charges in the presequence
    Vincenzo Zara
    Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Universita di Lecce, Via provinciale Lecce Monteroni, I 73100 Lecce, Italy
    J Mol Biol 365:958-67. 2007
    ..Following this observation, we found that several carrier proteins appear to carry non-cleavable presequences that may similarly act as charged intramolecular chaperones...
  8. ncbi request reprint Import of rat mitochondrial citrate carrier (CIC) at increasing salt concentrations promotes presequence binding to import receptor Tom20 and inhibits membrane translocation
    Vincenzo Zara
    Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Universita di Lecce, I 73100 Lecce, Italy
    J Cell Sci 118:3985-95. 2005
    ..We conclude that presequences can only act as mediators of mitochondrial protein import if they allow rapid release from import receptor sites. Release from receptors sites may be rate-limiting in translocation...
  9. ncbi request reprint Further insights into the assembly of the yeast cytochrome bc1 complex based on analysis of single and double deletion mutants lacking supernumerary subunits and cytochrome b
    Vincenzo Zara
    Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Universita di Lecce, Italy
    Eur J Biochem 271:1209-18. 2004
    ..Likewise, evidence of interactions between subunit 6, subunit 9 and cytochrome c1 suggests that a subcomplex between these two supernumerary subunits and the cytochrome might exist...
  10. doi request reprint Bcs1p can rescue a large and productive cytochrome bc(1) complex assembly intermediate in the inner membrane of yeast mitochondria
    Laura Conte
    Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Universita del Salento, Lecce, Italy
    Biochim Biophys Acta 1813:91-101. 2011
    ..This study identifies for the first time a productive assembly intermediate of the yeast bc(1) complex and gives new insights into the molecular mechanisms involved in the last steps of bc(1) assembly...
  11. pmc A krill oil supplemented diet suppresses hepatic steatosis in high-fat fed rats
    Alessandra Ferramosca
    Department of Biological and Environmental Sciences and Technologies, University of Salento, Lecce, Italy
    PLoS ONE 7:e38797. 2012
    ..Lastly, the KO supplementation prevented an increase in body weight, as well as oxidative damage of lipids and proteins, which is often found in high-fat fed animals...
  12. ncbi request reprint The mitochondrial tricarboxylate carrier of silver eel: dimeric structure and cytosolic exposure of both N- and C-termini
    Loredana Capobianco
    Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Universita di Lecce, via prov le Lecce Monteroni, 1 73100 Lecce, Italy
    J Protein Chem 21:515-21. 2002
    ..These findings suggest an arrangement of the dimeric tricarboxylate carrier into an even number of membrane-spanning domains, with the N-terminal and C-terminal regions oriented toward the intermembrane space of fish mitochondria...
  13. doi request reprint Dietary combination of conjugated linoleic acid (CLA) and pine nut oil prevents CLA-induced fatty liver in mice
    Alessandra Ferramosca
    Department of Biological and Environmental Sciences and Technologies, University of Salento, via prov le Lecce Monteroni, I 73100 Lecce, Italy
    J Agric Food Chem 56:8148-58. 2008
    ..Furthermore, lower levels of lipids, in comparison to values found in CLA-fed mice, were observed in the liver and plasma of CLA + P-fed animals...
  14. ncbi request reprint Identification and characterization of cytochrome bc(1) subcomplexes in mitochondria from yeast with single and double deletions of genes encoding cytochrome bc(1) subunits
    Vincenzo Zara
    Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Universita del Salento, Lecce, Italy
    FEBS J 274:4526-39. 2007
    ..The analysis of all the cytochrome bc(1) subcomplexes with monospecific antibodies directed against Bcs1p revealed that this chaperone protein is involved in a late stage of cytochrome bc(1) complex assembly...
  15. doi request reprint Oxidative stress negatively affects human sperm mitochondrial respiration
    Alessandra Ferramosca
    Department of Environmental and Biological Sciences and Technologies, University of Salento, Lecce, Italy
    Urology 82:78-83. 2013
    ..To correlate the level of oxidative stress in serum and seminal fluid and the level of sperm deoxyribonucleic acid (DNA) fragmentation with sperm mitochondrial respiratory efficiency...
  16. doi request reprint Biogenesis of mitochondrial carrier proteins: molecular mechanisms of import into mitochondria
    Alessandra Ferramosca
    Department of Environmental and Biological Sciences and Technologies, University of Salento, Lecce, Italy
    Biochim Biophys Acta 1833:494-502. 2013
    ..This review summarizes the present knowledge and discusses recent advances on the import and sorting of mitochondrial metabolite carriers...
  17. ncbi request reprint A protein structure prediction service in the ProGenGrid system
    Maria Mirto
    SPACI Consortium, University of Salento, Lecce and NNL CNR INFM, Lecce, Italy
    Stud Health Technol Inform 138:135-46. 2008
    ..The visualization of the predicted model is made possible by using an interactive virtual reality environment based on X3D and Ajax3d technologies...
  18. ncbi request reprint The mitochondrial tricarboxylate carrier of silver eel: chemical modification by sulfhydryl reagents
    Loredana Capobianco
    Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Universita di Lecce, via prov le Lecce Monteroni, I 73100 Lecce, Italy
    J Biochem Mol Biol 37:515-21. 2004
    ..The reactive cysteine(s) is most probably located at or near the substrate binding site of the carrier protein...
  19. ncbi request reprint Differential effects of high-carbohydrate and high-fat diets on hepatic lipogenesis in rats
    Alessandra Ferramosca
    Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Universita del Salento, Via provinciale Lecce Monteroni, 73100, Lecce, Italy
    Eur J Nutr 53:1103-14. 2014
    ..Hepatic fatty acid synthesis is influenced by several nutritional and hormonal factors. In this study, we have investigated the effects of distinct experimental diets enriched in carbohydrate or in fat on hepatic lipogenesis...
  20. doi request reprint Mitochondrial respiratory efficiency is positively correlated with human sperm motility
    Alessandra Ferramosca
    Department of Environmental and Biological Sciences and Technologies, University of Salento, Lecce, Italy
    Urology 79:809-14. 2012
    ..To correlate sperm mitochondrial respiratory efficiency with variations in sperm motility and with sperm morphologic anomalies...
  21. ncbi request reprint Olive oil increases the hepatic triacylglycerol content in mice by a distinct influence on the synthesis and oxidation of fatty acids
    Alessandra Ferramosca
    Department of Biological and Environmental Sciences and Technologies, University of Salento, Lecce Monteroni, Lecce, Italy
    Biosci Biotechnol Biochem 72:62-9. 2008
    ..This impairment of fatty acid oxidation, which was not apparent in corn oil-fed animals, may have had a role in the increase of hepatic lipid content found in the olive oil-fed mice...
  22. ncbi request reprint Modulation of hepatic steatosis by dietary fatty acids
    Alessandra Ferramosca
    Alessandra Ferramosca, Vincenzo Zara, Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Universita del Salento, I 73100 Lecce, Italy
    World J Gastroenterol 20:1746-55. 2014
    ..Quite interesting is the "case" of olive oil, since several studies have often provided different and/or conflicting results in animal models...
  23. ncbi request reprint Bioenergetics of mammalian sperm capacitation
    Alessandra Ferramosca
    Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Universita del Salento, Via provinciale Lecce Monteroni, 73100 Lecce, Italy
    Biomed Res Int 2014:902953. 2014
    ..Such knowledge, besides its obvious importance for basic science, could eventually translate into the development of novel strategies for treatment of male infertility, artificial reproduction, and sperm selection methods. ..
  24. ncbi request reprint Dietary fat and hepatic lipogenesis: mitochondrial citrate carrier as a sensor of metabolic changes
    Alessandra Ferramosca
    Department of Biological and Environmental Sciences and Technologies, University of Salento, Lecce, Italy
    Adv Nutr 5:217-25. 2014
    ..The crucial role of this protein is reinforced by the recent discovery of its involvement in other cellular processes, such as glucose-stimulated insulin secretion, inflammation, tumorigenesis, genome stability, and sperm metabolism...
  25. pmc The Rieske Iron-Sulfur Protein: Import and Assembly into the Cytochrome bc(1) Complex of Yeast Mitochondria
    Laura Conte
    Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Universita del Salento, via prov le Lecce Monteroni, 73100 Lecce, Italy
    Bioinorg Chem Appl 2011:363941. 2011
    ....