G Zanotti

Summary

Affiliation: University of Padova
Country: Italy

Publications

  1. ncbi Is tensegrity a unifying concept of protein folds?
    Giuseppe Zanotti
    Department of Organic Chemistry, University of Padua, Via Marzolo 1, 35131 Padua, Italy
    FEBS Lett 534:7-10. 2003
  2. ncbi Structure of zebra fish HIUase: insights into evolution of an enzyme to a hormone transporter
    Giuseppe Zanotti
    Department of Chemistry, University of Padua, and ICTB, Section of Padua, Via Marzolo 1 35131 Padova, Italy
    J Mol Biol 363:1-9. 2006
  3. ncbi Structure of the neutrophil-activating protein from Helicobacter pylori
    Giuseppe Zanotti
    Dipartimento di Chimica Organica e Centro CNR Biopolimeri, Universita di Padova, Via Marzolo 1, 35131, Padova, Italy
    J Mol Biol 323:125-30. 2002
  4. ncbi Structural and mutational analyses of protein-protein interactions between transthyretin and retinol-binding protein
    Giuseppe Zanotti
    Department of Chemical Sciences and Institute of Biomolecular Chemistry CNR, University of Padua, Italy
    FEBS J 275:5841-54. 2008
  5. ncbi Structure of chicken plasma retinol-binding protein
    G Zanotti
    Department of Organic Chemistry, University of Padua and Biopolymer Research Center, CNR, Padua, Italy
    Biochim Biophys Acta 1550:64-9. 2001
  6. ncbi Structure of the trigonal crystal form of bovine annexin IV
    G Zanotti
    Department of Organic Chemistry, University of Padova, and Biopolymer Research Center, C N R, 35131 Padova, Italy
    Biochem J 329:101-6. 1998
  7. ncbi Helicobacter pylori acidic stress response factor HP1286 is a YceI homolog with new binding specificity
    Lorenza Sisinni
    Department of Biological Chemistry, University of Padua, Padua, Italy
    FEBS J 277:1896-905. 2010
  8. ncbi Structure of sulfur-substituted rhodanese at 1.36 A resolution
    F Gliubich
    Department of Organic Chemistry, University of Padova and Biopolymer Research Center, CNR, 35131, Padova, Italy
    Acta Crystallogr D Biol Crystallogr 54:481-6. 1998
  9. ncbi Crystal structure of chicken liver basic fatty acid-binding protein at 2.7 A resolution
    G Scapin
    Department of Organic Chemistry, University of Padova, Italy
    Mol Cell Biochem 98:95-9. 1990
  10. ncbi Structure of pig plasma retinol-binding protein at 1.65 A resolution
    G Zanotti
    Department of Organic Chemistry, University of Padova and Biopolymer Research Center, CNR, 35131 Padova, Italy
    Acta Crystallogr D Biol Crystallogr 54:1049-52. 1998

Collaborators

Detail Information

Publications54

  1. ncbi Is tensegrity a unifying concept of protein folds?
    Giuseppe Zanotti
    Department of Organic Chemistry, University of Padua, Via Marzolo 1, 35131 Padua, Italy
    FEBS Lett 534:7-10. 2003
    ..This latter property well explains both the folding process and the behavior of globular proteins under mild denaturing conditions, as revealed by the molten globule state...
  2. ncbi Structure of zebra fish HIUase: insights into evolution of an enzyme to a hormone transporter
    Giuseppe Zanotti
    Department of Chemistry, University of Padua, and ICTB, Section of Padua, Via Marzolo 1 35131 Padova, Italy
    J Mol Biol 363:1-9. 2006
    ..The evolution of TTR has been accompanied by remarkable changes of the HIUase active sites that gave rise to a channel open at both ends, thus allowing free access to hormone molecules...
  3. ncbi Structure of the neutrophil-activating protein from Helicobacter pylori
    Giuseppe Zanotti
    Dipartimento di Chimica Organica e Centro CNR Biopolimeri, Universita di Padova, Via Marzolo 1, 35131, Padova, Italy
    J Mol Biol 323:125-30. 2002
    ..This is due to the presence of a large number of positively charged residues, which could well account for its unique ability in activating human leukocytes...
  4. ncbi Structural and mutational analyses of protein-protein interactions between transthyretin and retinol-binding protein
    Giuseppe Zanotti
    Department of Chemical Sciences and Institute of Biomolecular Chemistry CNR, University of Padua, Italy
    FEBS J 275:5841-54. 2008
    ....
  5. ncbi Structure of chicken plasma retinol-binding protein
    G Zanotti
    Department of Organic Chemistry, University of Padua and Biopolymer Research Center, CNR, Padua, Italy
    Biochim Biophys Acta 1550:64-9. 2001
    ..A distinctive feature of the avian protein is a better definition of the loop 63-67, close to the opening of the beta-barrel cavity accommodating the retinol molecule, which is rather disordered in the structures of mammalian RBPs...
  6. ncbi Structure of the trigonal crystal form of bovine annexin IV
    G Zanotti
    Department of Organic Chemistry, University of Padova, and Biopolymer Research Center, C N R, 35131 Padova, Italy
    Biochem J 329:101-6. 1998
    ..The propensity of several annexins to generate similar trimeric arrays supports the hypothesis that trimeric complexes of such annexins, including annexin IV, may represent the functional units that interact with membranes...
  7. ncbi Helicobacter pylori acidic stress response factor HP1286 is a YceI homolog with new binding specificity
    Lorenza Sisinni
    Department of Biological Chemistry, University of Padua, Padua, Italy
    FEBS J 277:1896-905. 2010
    ....
  8. ncbi Structure of sulfur-substituted rhodanese at 1.36 A resolution
    F Gliubich
    Department of Organic Chemistry, University of Padova and Biopolymer Research Center, CNR, 35131, Padova, Italy
    Acta Crystallogr D Biol Crystallogr 54:481-6. 1998
    ..Only limited changes of the enzyme structure have been found as a result of the drastic change in the crystal medium...
  9. ncbi Crystal structure of chicken liver basic fatty acid-binding protein at 2.7 A resolution
    G Scapin
    Department of Organic Chemistry, University of Padova, Italy
    Mol Cell Biochem 98:95-9. 1990
    ..The structure of this liver protein appears very similar to that of the Escherichia coli derived rat intestinal FABP recently determined by X-ray diffraction methods...
  10. ncbi Structure of pig plasma retinol-binding protein at 1.65 A resolution
    G Zanotti
    Department of Organic Chemistry, University of Padova and Biopolymer Research Center, CNR, 35131 Padova, Italy
    Acta Crystallogr D Biol Crystallogr 54:1049-52. 1998
    ....
  11. ncbi Muscle fatty acid-binding protein
    G Zanotti
    Dipartimento Chimica Organica, Università di Padova e Centro Studi Biopolimeri del CNR, Via Marzolo 1, 35131, Padova, Italy
    Biochim Biophys Acta 1441:94-105. 1999
    ..The three-dimensional structure of different fatty acid-protein complexes along with molecular dynamics simulations are now providing insight into the molecular details of the specificity of the ligand binding...
  12. ncbi Active site structural features for chemically modified forms of rhodanese
    F Gliubich
    Department of Organic Chemistry, University of Padova and Biopolymer Research Center, Consiglio Nazionale delle Ricerche, 35131 Padova, Italy
    J Biol Chem 271:21054-61. 1996
    ....
  13. ncbi Crystal structure of liganded and unliganded forms of bovine plasma retinol-binding protein
    G Zanotti
    Department of Organic Chemistry, University of Padova, Italy
    J Biol Chem 268:10728-38. 1993
    ....
  14. ncbi Crystal structure of CagZ, a protein from the Helicobacter pylori pathogenicity island that encodes for a type IV secretion system
    Laura Cendron
    Dipartmento di Scienze Chimiche, Istituto de Chimica Biomoleculare del CNR, Universita di Padova, Padua, Italy
    J Mol Biol 340:881-9. 2004
    ..pylori type IV secretion system on the cytoplasmic side of the inner membrane...
  15. ncbi The crystal structure of the superoxide dismutase from Helicobacter pylori reveals a structured C-terminal extension
    Luciana Esposito
    Institute of Biostructures and Bioimaging, CNR, Via Mezzocannone 16, I 80134, Naples, Italy
    Biochim Biophys Acta 1784:1601-6. 2008
    ..The structure of extended carboxyl termini is discussed in light of putative functions it may serve...
  16. ncbi Crystal structure and refolding properties of the mutant F99S/M153T/V163A of the green fluorescent protein
    R Battistutta
    Dipartimento di Chimica Organica and Centro Studi sui Biopolimeri, Universita di Padova, Padova, Italy
    Proteins 41:429-37. 2000
    ..We conclude that the higher fluorescence activity of c3-GFP can be attributed neither to particular structural features nor to a faster folding process, as previously proposed...
  17. ncbi Three-dimensional structure of recombinant human muscle fatty acid-binding protein
    G Zanotti
    Department of Organic Chemistry, University of Padova, Italy
    J Biol Chem 267:18541-50. 1992
    ..These findings suggest that the intestinal and muscle fatty acid-binding proteins have evolved distinct binding sites in order to satisfy different requirements within the tissues where they are expressed...
  18. ncbi Structure at 1.44 A resolution of an N-terminally truncated form of the rat serum complement C3d fragment
    G Zanotti
    Department of Organic Chemistry and Biopolymer Research Center, University of Padova, Italy
    Biochim Biophys Acta 1478:232-8. 2000
    ..The truncation at the N-terminus of C3d leads to the exposure of a surface of the molecule that favours dimerisation, so that in both crystal forms, the fragment is present as a dimer, with monomers related by a two-fold axis...
  19. ncbi ATP site-directed inhibitors of protein kinase CK2: an update
    S Sarno
    Department of Biological Chemistry and CNR Institute of Neurosciences, University of Padova, Italy
    Curr Top Med Chem 11:1340-51. 2011
    ....
  20. ncbi Inspecting the structure-activity relationship of protein kinase CK2 inhibitors derived from tetrabromo-benzimidazole
    Roberto Battistutta
    Department of Chemistry, University of Padua, Via Marzolo 1, 35131 Padua, Italy
    Chem Biol 12:1211-9. 2005
    ....
  21. ncbi Stop the killer: how to inhibit the anthrax lethal factor metalloprotease
    Cesare Montecucco
    Istituto Veneto di Medicina Molecolare, Via Orus, 35121 Padua, Italy
    Trends Biochem Sci 29:282-5. 2004
    ..This will pave the way for design of novel anthrax therapeutics that are capable of inhibiting the metalloprotease activity of LF in vivo...
  22. ncbi Crystal structure of peach Pru p 3, the prototypic member of the family of plant non-specific lipid transfer protein pan-allergens
    Nicola Pasquato
    Department of Chemistry, University of Padua, and ICTB, Section of Padua, Via Marzolo 1, 35131 Padova, Italy
    J Mol Biol 356:684-94. 2006
    ....
  23. ncbi The structure of 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase provides insights into the mechanism of uric acid degradation
    Laura Cendron
    Department of Chemistry, University of Padua, and Instituto di Chimica Biomolecolare Consiglio Nazionale delle Ricerche, Section of Padua, Via Marzolo 1, 35131 Padua, Italy
    J Biol Chem 282:18182-9. 2007
    ..The structural and functional characterization of 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase can provide useful information in view of the potential use of this enzyme in the enzymatic therapy of gout...
  24. ncbi Functional and structural aspects of Helicobacter pylori acidic stress response factors
    Giuseppe Zanotti
    Department of Biological Chemistry, University of Padua, Viale G Colombo 3, 35121 Padua, Italy
    IUBMB Life 62:715-23. 2010
    ..In this review, we discuss the molecular and functional aspects of some of the proteins that are commonly found overexpressed during the acid stress...
  25. ncbi The ATP-binding site of protein kinase CK2 holds a positive electrostatic area and conserved water molecules
    Roberto Battistutta
    Department of Chemical Sciences, University of Padova, Via Marzolo 1, 35131 Padova, Italy roberto
    Chembiochem 8:1804-9. 2007
    ..The presence of this positive region and two unique bulky residues that are typical of CK2, Ile66 and Ile174, play a critical role in determining the ligand orientation and binding selectivity...
  26. ncbi Mining overrepresented 3D patterns of secondary structures in proteins
    Matteo Comin
    Department of Information Engineering, University of Padova, Padova, Italy
    J Bioinform Comput Biol 6:1067-87. 2008
    ..We analyze the results of our method on a dataset of 300 nonredundant proteins. Supplementary material is available at (http://www.dei.unipd.it/nciompin/papers/CGZ-jbcb-suppl.pdf/)...
  27. ncbi Cloning of human 3-hydroxyanthranilic acid dioxygenase in Escherichia coli: characterisation of the purified enzyme and its in vitro inhibition by Zn2+
    Vito Calderone
    Department of Organic Chemistry and Biopolymer Research Center, University of Padua, Via Marzolo 1, 35131, Padua, Italy
    Biochim Biophys Acta 1596:283-92. 2002
    ..A circular dichroism analysis has also been performed, showing that the secondary structure is mainly of the beta type, with a minority of alpha...
  28. ncbi High-resolution structures of retinol-binding protein in complex with retinol: pH-induced protein structural changes in the crystal state
    Vito Calderone
    Department of Organic Chemistry, University of Padova, and Biopolymer Research Center, Italian National Research Council, Via Marzolo 1, 35131, Padova, Italy
    J Mol Biol 329:841-50. 2003
    ....
  29. ncbi Toward the rational design of protein kinase casein kinase-2 inhibitors
    Stefania Sarno
    Department of Biological Chemistry, CNR Biomembrane Research Center, University of Padova, Viale G. Colombo 3, 35121, Padova, Italy
    Pharmacol Ther 93:159-68. 2002
    ..The data presented pave the road toward the rational design of more potent and selective inhibitors of CK2 and the generation of CK2 mutants refractory to inhibition, useful to probe the implication of CK2 in specific cellular functions...
  30. ncbi The Helicobacter pylori CagD (HP0545, Cag24) protein is essential for CagA translocation and maximal induction of interleukin-8 secretion
    Laura Cendron
    Department of Chemistry, University of Padua Via Marzolo 1, I 35131 Padua, Italy
    J Mol Biol 386:204-17. 2009
    ....
  31. ncbi Identification and structural analysis of a zebrafish apo and holo cellular retinol-binding protein
    Vito Calderone
    Department of Organic Chemistry, University of Padova, and Biopolymer Research Center, Italian National Research Council, Via Marzolo 1, 35131, Padova, Italy
    J Mol Biol 321:527-35. 2002
    ....
  32. ncbi Acidic pH-induced conformational changes in amyloidogenic mutant transthyretin
    Nicola Pasquato
    Department of Chemistry, University of Padua, and ICTB, Section of Padua, Via Marzolo 1, 35131 Padova, Italy
    J Mol Biol 366:711-9. 2007
    ..This asymmetry and a possible destabilization of the tetrameric quaternary structure of TTR may be responsible for the amyloidogenic potential of the two TTR mutant forms at low pH...
  33. ncbi Inhibition of protein kinase CK2 by anthraquinone-related compounds. A structural insight
    Erika De Moliner
    Department of Organic Chemistry, University of Padova, Padova 35131, Italy
    J Biol Chem 278:1831-6. 2003
    ....
  34. ncbi Amyloidogenic potential of transthyretin variants: insights from structural and computational analyses
    Laura Cendron
    Department of Biological Chemistry, University of Padua, and Istituto di Chimica Biomolecolare, Section of Padua, Viale G Colombo 3, 35121 Padua, Italy
    J Biol Chem 284:25832-41. 2009
    ....
  35. ncbi Crystal structure of the PsbQ protein of photosystem II from higher plants
    Vito Calderone
    Dipartimento di Chimica Organica e Centro Biopolimeri del CNR, , Via Marzolo 1, 35131 Padua, Italy
    EMBO Rep 4:900-5. 2003
    ..The distribution of positive and negative charges on the protein surface might explain the ability of PsbQ to increase the binding of Cl(-) and Ca(2+) and make them available to PSII...
  36. ncbi Structure of two iron-binding proteins from Bacillus anthracis
    Elena Papinutto
    Dipartimento di Chimica Organica e Centro CNR Biopolimeri, Universita di Padova, Via Marzolo 1, 35131 Padova, Italy
    J Biol Chem 277:15093-8. 2002
    ..They are sphere-like proteins with an internal cavity. We also show that they act as ferritins and are thus involved in iron uptake and regulation, a fundamental function during bacterial growth...
  37. ncbi Crystal structure of amine oxidase from bovine serum
    Michele Lunelli
    Department of Biological Chemistry, University of Padua, Viale G Colombo 3, 35121 Padua, Italy
    J Mol Biol 346:991-1004. 2005
    ....
  38. ncbi Crystal structure of antigen TpF1 from Treponema pallidum
    Anton Thumiger
    Dipartimento di Scienze Chimiche e ICB-CNR, , Padova, Italy
    Proteins 62:827-30. 2006
  39. ncbi Crystal structure of alkyl hydroperoxide-reductase (AhpC) from Helicobacter pylori
    Elena Papinutto
    Department of Chemical Sciences, University of Padua, and ICTB, Via Marzolo 1, and Venetian Institute of Molecular Medicine, Via Orus 2, Padua, Italy
    Biochim Biophys Acta 1753:240-6. 2005
    ..Oligomerization properties of AhpC have been also characterized by gel filtration chromatography...
  40. ncbi Plasma retinol-binding protein: structure and interactions with retinol, retinoids, and transthyretin
    Giuseppe Zanotti
    Department of Organic Chemistry, University of Padova, 35131 Padua, Italy
    Vitam Horm 69:271-95. 2004
    ..Overall, the available evidence indicates the existence of a high degree of complementarity between RBP and TTR, the contact areas of which are highly sensitive to conformational changes and amino acid replacements...
  41. ncbi The neutrophil-activating protein of Helicobacter pylori
    William G Dundon
    Centro CNR Biomembrane e Dipartimento di Scienze Biomediche, Universita di Padova, Italy
    Int J Med Microbiol 291:545-50. 2002
    ..Sequence analysis of the genomes of several other bacteria reveal that many possess Dps/HP-NAP-like proteins. The preliminary characterisation of some of these proteins will be discussed...
  42. ncbi Structural and mutational analysis of TenA protein (HP1287) from the Helicobacter pylori thiamin salvage pathway - evidence of a different substrate specificity
    Nicola Barison
    Department of Biological Chemistry, University of Padua, Italy
    FEBS J 276:6227-35. 2009
    ..pylori enzyme in the thiamin biosynthetic route, which is in agreement with the relevance of this protein in the stomach colonization process...
  43. ncbi Borrelia burgdorferi NapA-driven Th17 cell inflammation in lyme arthritis
    Gaia Codolo
    Venetian Institute of Molecular Medicine, and University of Padua, Padua, Italy
    Arthritis Rheum 58:3609-17. 2008
    ..This study was undertaken to evaluate the role of the innate and acquired immune responses elicited by the neutrophil-activating protein A (NapA) of B burgdorferi in patients with Lyme arthritis...
  44. ncbi Crystallization and preliminary X-ray data of amine oxidase from bovine serum
    Vito Calderone
    Department of Organic Chemistry, University of Padova, Italy
    Acta Crystallogr D Biol Crystallogr 59:727-9. 2003
    ..The crystals obtained belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 77.68, b = 131.19, c = 134.00 A, and diffract to at least 2.4 A resolution. BSAO is the first mammalian amine oxidase to be crystallized...
  45. ncbi Three-dimensional atomic structure of a catalytic subunit mutant of human protein kinase CK2
    Eugenia Pechkova
    Fondazione E L B A, Rome, Italy
    Acta Crystallogr D Biol Crystallogr 59:2133-9. 2003
    ..A comparison between the human protein kinase CK2alpha and the corresponding enzyme from a lower organism (Zea mays) is made...
  46. ncbi Distinctive binding and structural properties of piscine transthyretin
    Claudia Folli
    Department of Biochemistry and Molecular Biology, University of Parma, Parco Area delle Scienze 23/A, 43100 Parma, Italy
    FEBS Lett 555:279-84. 2003
    ..Overall, this study makes it possible to draw conclusions on evolutionary relationships for RBPs and TTRs of phylogenetically distant vertebrates...
  47. ncbi Logical identification of an allantoinase analog (puuE) recruited from polysaccharide deacetylases
    Ileana Ramazzina
    Department of Biochemistry and Molecular Biology and Mathematics, University of Parma, 43100, Parma, Italy
    J Biol Chem 283:23295-304. 2008
    ..These data provide a structural rationale for the shifting of substrate specificity that occurred during evolution...
  48. ncbi Ligand binding and structural analysis of a human putative cellular retinol-binding protein
    Claudia Folli
    Department of Biochemistry and Molecular Biology, University of Parma, Italy
    J Biol Chem 277:41970-7. 2002
    ..These results indicate that human CRBP IV belongs to a clearly distinct CRBP subfamily and suggest a relatively different mode of retinol binding for this binding protein...
  49. ncbi Biochemical and three-dimensional-structural study of the specific inhibition of protein kinase CK2 by [5-oxo-5,6-dihydroindolo-(1,2-a)quinazolin-7-yl]acetic acid (IQA)
    Stefania Sarno
    Venetian Institute for Molecular Medicine (VIMM, University of Padova, 35121 Padova, Italy
    Biochem J 374:639-46. 2003
    ..These results provide new tools for deciphering the enigmatic role of CK2 in living cells and may pave the way for the development of drugs depending on CK2 activity...
  50. ncbi Features and potentials of ATP-site directed CK2 inhibitors
    Stefania Sarno
    Department of Biological Chemistry, University of Padova, Viale G. Colombo 3, 35129 Padova, Italy
    Biochim Biophys Acta 1754:263-70. 2005
    ....
  51. ncbi Multiple binding sites for substrates and modulators of semicarbazide-sensitive amine oxidases: kinetic consequences
    Andrew Holt
    Department of Pharmacology, Faculty of Medicine and Dentistry, 9 70 Medical Sciences Bldg, University of Alberta, Edmonton, AB, Canada
    Mol Pharmacol 73:525-38. 2008
    ....
  52. ncbi Functional and structural characterization of ovine ornithine transcarbamoylase
    Ambra De Gregorio
    Department of Organic and Biological Chemistry, University of Messina, Salita Sperone 31, 98166 Messina, Italy
    Org Biomol Chem 1:3178-85. 2003
    ....
  53. ncbi Global secondary structure packing angle bias in proteins
    Daniel E Platt
    Bioinformatics and Pattern Discovery, IBM T J Watson Research Center, Yorktown Heights, New York, USA
    Proteins 53:252-61. 2003
    ..Selection for interactive segment triplets shows results consistent with prior studies...
  54. ncbi Novel pathogenic mechanisms of congenital insensitivity to pain with anhidrosis genetic disorder unveiled by functional analysis of neurotrophic tyrosine receptor kinase type 1/nerve growth factor receptor mutations
    Claudia Miranda
    Department of Experimental Oncology, Istituto Nazionale Tumori, Via G. Venezian 1, Milan 20133, Italy
    J Biol Chem 277:6455-62. 2002
    ....