Margherita Zanetti

Summary

Affiliation: University of Trieste
Country: Italy

Publications

  1. ncbi request reprint Biological characterization of two novel cathelicidin-derived peptides and identification of structural requirements for their antimicrobial and cell lytic activities
    B Skerlavaj
    Dipartimento di Scienze e Tecnologie Biomediche, Universita di Udine, I 33100 Udine, Italy
    J Biol Chem 271:28375-81. 1996
  2. ncbi request reprint Cathelicidins, multifunctional peptides of the innate immunity
    Margherita Zanetti
    Department of Biomedical Sciences and Technology, University of Udine, I 33100 Udine, Italy
    J Leukoc Biol 75:39-48. 2004
  3. ncbi request reprint The role of cathelicidins in the innate host defenses of mammals
    Margherita Zanetti
    Department of Biomedical Sciences and Technology, University of Udine, 1 33100 Udine, Italy
    Curr Issues Mol Biol 7:179-96. 2005
  4. ncbi request reprint In vitro and in vivo antimicrobial activity of two alpha-helical cathelicidin peptides and of their synthetic analogs
    Monica Benincasa
    Department of Biochemistry, Biophysics and Macromolecular Chemistry, University of Trieste, Via Giorgieri, 1, I 34127 Trieste, Italy
    Peptides 24:1723-31. 2003
  5. ncbi request reprint Cathelicidin peptides as candidates for a novel class of antimicrobials
    Margherita Zanetti
    Dipartimento di Scienze e Tecnologie Biomediche, Universit di Udine, P le Kolbe, 4, 33100 Udine, Italy
    Curr Pharm Des 8:779-93. 2002
  6. pmc Broad-spectrum activity against bacterial mastitis pathogens and activation of mammary epithelial cells support a protective role of neutrophil cathelicidins in bovine mastitis
    Linda Tomasinsig
    Department of Biomedical Sciences and Technology, University of Udine, Udine, Italy
    Infect Immun 78:1781-8. 2010
  7. doi request reprint Comparative activity and mechanism of action of three types of bovine antimicrobial peptides against pathogenic Prototheca spp
    Linda Tomasinsig
    Department of Medical and Biological Sciences, University of Udine, Piazzale Kolbe 4, 33100 Udine, Italy
    J Pept Sci 18:105-13. 2012
  8. ncbi request reprint Structural and functional characterization of hBD-1(Ser35), a peptide deduced from a DEFB1 polymorphism
    Raffaella Circo
    Dipartimento di Scienze e Tecnologie Biomediche, Universita di Udine, P le Kolbe, 4, 33100 Udine, Italy
    Biochem Biophys Res Commun 293:586-92. 2002
  9. pmc The human cathelicidin LL-37 modulates the activities of the P2X7 receptor in a structure-dependent manner
    Linda Tomasinsig
    Department of Biomedical Sciences and Technology, University of Udine, 33100 Udine, Italy
    J Biol Chem 283:30471-81. 2008
  10. doi request reprint Structure dependence of biological activities for primate cathelicidins
    Linda Tomasinsig
    Department of Biomedical Sciences and Technology, University of Udine, Udine, Italy
    J Pept Sci 15:576-82. 2009

Collaborators

Detail Information

Publications29

  1. ncbi request reprint Biological characterization of two novel cathelicidin-derived peptides and identification of structural requirements for their antimicrobial and cell lytic activities
    B Skerlavaj
    Dipartimento di Scienze e Tecnologie Biomediche, Universita di Udine, I 33100 Udine, Italy
    J Biol Chem 271:28375-81. 1996
    ..The involvement of the C-terminal hydrophobic tail in the cytotoxic activity has been further demonstrated by inducing a major loss of activity in an analogue after replacing highly hydrophobic residues with more hydrophilic ones...
  2. ncbi request reprint Cathelicidins, multifunctional peptides of the innate immunity
    Margherita Zanetti
    Department of Biomedical Sciences and Technology, University of Udine, I 33100 Udine, Italy
    J Leukoc Biol 75:39-48. 2004
    ..These multiple actions provide evidence for active participation of cathelicidin peptides in the regulation of the antimicrobial host defenses...
  3. ncbi request reprint The role of cathelicidins in the innate host defenses of mammals
    Margherita Zanetti
    Department of Biomedical Sciences and Technology, University of Udine, 1 33100 Udine, Italy
    Curr Issues Mol Biol 7:179-96. 2005
    ....
  4. ncbi request reprint In vitro and in vivo antimicrobial activity of two alpha-helical cathelicidin peptides and of their synthetic analogs
    Monica Benincasa
    Department of Biochemistry, Biophysics and Macromolecular Chemistry, University of Trieste, Via Giorgieri, 1, I 34127 Trieste, Italy
    Peptides 24:1723-31. 2003
    ..The parent peptides and mBMAP-28 analog protected mice from lethal i.p. infections in an acute peritonitis model at peptide doses significantly lower than those toxic to the animals, suggesting a satisfactory therapeutic index...
  5. ncbi request reprint Cathelicidin peptides as candidates for a novel class of antimicrobials
    Margherita Zanetti
    Dipartimento di Scienze e Tecnologie Biomediche, Universit di Udine, P le Kolbe, 4, 33100 Udine, Italy
    Curr Pharm Des 8:779-93. 2002
    ..This review focuses on recent studies on the structure and in vitro and in vivo biological activities of these peptides...
  6. pmc Broad-spectrum activity against bacterial mastitis pathogens and activation of mammary epithelial cells support a protective role of neutrophil cathelicidins in bovine mastitis
    Linda Tomasinsig
    Department of Biomedical Sciences and Technology, University of Udine, Udine, Italy
    Infect Immun 78:1781-8. 2010
    ..Our results indicate multiple roles for the bovine cathelicidins in mastitis, with complementary and mutually enhanced antimicrobial activities against causative pathogens and the capacity to activate host cells...
  7. doi request reprint Comparative activity and mechanism of action of three types of bovine antimicrobial peptides against pathogenic Prototheca spp
    Linda Tomasinsig
    Department of Medical and Biological Sciences, University of Udine, Piazzale Kolbe 4, 33100 Udine, Italy
    J Pept Sci 18:105-13. 2012
    ..In addition, the ability of Bac5 and LAP to act via non-lytic mechanisms may be exploited for the development of target-selective drugs...
  8. ncbi request reprint Structural and functional characterization of hBD-1(Ser35), a peptide deduced from a DEFB1 polymorphism
    Raffaella Circo
    Dipartimento di Scienze e Tecnologie Biomediche, Universita di Udine, P le Kolbe, 4, 33100 Udine, Italy
    Biochem Biophys Res Commun 293:586-92. 2002
    ..This activity likely depends on the ability of hBD-1(Ser35) to adopt an amphipathic conformation in hydrophobic environment, similar to the wild type peptide, as suggested by CD spectroscopy...
  9. pmc The human cathelicidin LL-37 modulates the activities of the P2X7 receptor in a structure-dependent manner
    Linda Tomasinsig
    Department of Biomedical Sciences and Technology, University of Udine, 33100 Udine, Italy
    J Biol Chem 283:30471-81. 2008
    ..Our results indicate that P2X(7) is involved in the proliferative cell response to LL-37 and that the structural/aggregational properties of LL-37 determine its capacity to modulate the activation state of P2X(7)...
  10. doi request reprint Structure dependence of biological activities for primate cathelicidins
    Linda Tomasinsig
    Department of Biomedical Sciences and Technology, University of Udine, Udine, Italy
    J Pept Sci 15:576-82. 2009
    ..Our results suggest that different evolutionary trajectories for each orthologue lead to distinct sets of physical characteristics, which significantly differentiates their biological activities...
  11. ncbi request reprint Mechanistic and functional studies of the interaction of a proline-rich antimicrobial peptide with mammalian cells
    Linda Tomasinsig
    Department of Biomedical Sciences and Technology, University of Udine, P le Kolbe 4, I 33100 Udine, Italy
    J Biol Chem 281:383-91. 2006
    ..Further investigations aimed to reveal functional implications of intracellular uptake of Bac7(1-35) demonstrated that it correlates with enhanced S phase entry of 3T3 cells, indicating a novel function for this proline-rich peptide...
  12. pmc Genome-wide transcriptional profiling of the Escherichia coli response to a proline-rich antimicrobial peptide
    Linda Tomasinsig
    Department of Biomedical Sciences and Technology, University of Udine, P le Kolbe 4, I 33100 Udine, Italy
    Antimicrob Agents Chemother 48:3260-7. 2004
    ..The generation of a transcriptional response under the experimental conditions used is consistent with the ability of Bac7(1-35) to interact with bacterial components and affect biological processes in this organism...
  13. doi request reprint Modulation of cytokine gene expression by cathelicidin BMAP-28 in LPS-stimulated and -unstimulated macrophages
    Francesca D'Este
    Department of Medical and Biological Sciences, University of Udine, Udine, Italy
    Immunobiology 217:962-71. 2012
    ....
  14. ncbi request reprint Antimicrobial activity of SMAP-29 against the Bacteroides fragilis group and clostridia
    Alessandra Arzese
    Institute of Microbiology, Udine Medical School, University of Udine, p zza S M Misericordia 1, 33100 Udine, Italy
    J Antimicrob Chemother 52:375-81. 2003
    ..In this study, the effects of the peptide against the Bacteroides fragilis group, including antibiotic-resistant isolates, Clostridium perfringens and Clostridium difficile reference and clinical isolates, were investigated...
  15. pmc BMAP-28, an antibiotic peptide of innate immunity, induces cell death through opening of the mitochondrial permeability transition pore
    Angela Risso
    Department of Biotechnology and Biomedical Sciences, University of Udine, Via Cotonificio 108, I 33100 Udine, Italy
    Mol Cell Biol 22:1926-35. 2002
    ..Our results demonstrate that BMAP-28 is an inducer of the mitochondrial permeability transition pore and that its cytotoxic potential depends on its effects on mitochondrial permeability...
  16. ncbi request reprint Pro-rich antimicrobial peptides from animals: structure, biological functions and mechanism of action
    Renato Gennaro
    Department of Biochemistry, Biophysics and Macromolecular Chemistry, University of Trieste, Via Giorgieri, 1, I 34127 Trieste, Italy
    Curr Pharm Des 8:763-78. 2002
    ....
  17. ncbi request reprint Inducible expression of an antimicrobial peptide of the innate immunity in polymorphonuclear leukocytes
    Linda Tomasinsig
    Dipartimento di Scienze e Tecnologie Biomediche, Universita di Udine, Italy
    J Leukoc Biol 72:1003-10. 2002
    ..Collectively, these results provide the first evidence that fully differentiated neutrophils are capable of de novo synthesis and secretion of a granule-associated antimicrobial peptide...
  18. doi request reprint BMAP-28 improves the efficacy of vancomycin in rat models of gram-positive cocci ureteral stent infection
    Fiorenza Orlando
    Experimental Animal Models for Aging Units, Research Department, I N R C A I R R C S, Ancona, Italy
    Peptides 29:1118-23. 2008
    ..BMAP-28 combined with vancomycin showed efficacies higher than that of each single compound. These results highlight the potential usefulness of this combination in preventing ureteral stent-associated in gram-positive infections...
  19. doi request reprint Efficacy of LL-37 and granulocyte colony-stimulating factor in a neutropenic murine sepsis due to Pseudomonas aeruginosa
    Oscar Cirioni
    Institute of Infectious Diseases and Public Health, Department of General Surgery, I N R C A I R R C S Universita Politecnica delle Marche, Ancona, Italy
    Shock 30:443-8. 2008
    ..Combination therapy between LL-37 and G-CSF is a promising therapeutic strategy for the management of severe Pseudomonas infection complicated by neutropenia...
  20. ncbi request reprint RNAIII-inhibiting peptide in combination with the cathelicidin BMAP-28 reduces lethality in mouse models of staphylococcal sepsis
    Roberto Ghiselli
    Department of General Surgery, Istituto Nazionale Riposo e Cura Anziani Istituto di Ricovero e Cura a Carattere Scientifico, Universita Politecnica delle Marche, Ancona, Italy
    Shock 26:296-301. 2006
    ..These results highlight the capacity of RIP and BMAP-28 to reduce the septic effects of bacterial cell components and exotoxins, and suggest their potential use in the treatment of severe staphylococcus-associated sepsis...
  21. ncbi request reprint Effects of the antimicrobial peptide BMAP-27 in a mouse model of obstructive jaundice stimulated by lipopolysaccharide
    Roberto Ghiselli
    Department of General Surgery, I N R C A I R R C S, Universita Politecnica delle Marche, Ancona, Italy
    Peptides 27:2592-9. 2006
    ..In light of its dual antimicrobial and anti-endotoxin properties, BMAP-27 could be an interesting compound to inhibit bacterial translocation and endotoxin release in obstructive jaundice...
  22. pmc LL-37 protects rats against lethal sepsis caused by gram-negative bacteria
    Oscar Cirioni
    Universita Politecnica delle Marche, Clinica Malattie Infettive, c o Ospedale Regionale, Via Conca 71, 60020 Torrette, Ancona, Italy
    Antimicrob Agents Chemother 50:1672-9. 2006
    ..LL-37 and imipenem proved to be the most effective treatments in reducing all variables measured. Due to its multifunctional properties, LL-37 may become an important future consideration for the treatment of sepsis...
  23. ncbi request reprint Pre-treatment of central venous catheters with the cathelicidin BMAP-28 enhances the efficacy of antistaphylococcal agents in the treatment of experimental catheter-related infection
    Oscar Cirioni
    Institute of Infectious Diseases and Public Health, Universita Politecnica delle Marche, Ancona, Italy
    Peptides 27:2104-10. 2006
    ..These results suggest that CVC pre-treated with BMAP-28 represents an attractive choice for the treatment of device-related infections caused by staphylococci...
  24. ncbi request reprint The antimicrobial peptide BMAP-28 reduces lethality in mouse models of staphylococcal sepsis
    Andrea Giacometti
    Institute of Infectious Diseases and Public Health, Universita Politecnica delle Marche, Ancona, Italy
    Crit Care Med 32:2485-90. 2004
    ..A mouse model of staphylococcal sepsis was used to compare the efficacy of the bovine antimicrobial peptide BMAP-28, a compound of the cathelicidin family, with that of conventional antibiotics...
  25. ncbi request reprint In vitro activity of protegrin-1 and beta-defensin-1, alone and in combination with isoniazid, against Mycobacterium tuberculosis
    Lanfranco Fattorini
    Dipartimento di Malattie Infettive, Parassitarie e Immunomediate, Istituto Superiore di Sanita, Rome, Italy
    Peptides 25:1075-7. 2004
    ..The combination of PG-1 or HBD-1 with isoniazid significantly reduced M. tuberculosis growth in comparison with the peptides or isoniazid alone...
  26. ncbi request reprint In vitro effect on Cryptosporidium parvum of short-term exposure to cathelicidin peptides
    Andrea Giacometti
    Institute of Infectious Diseases and Public Health, University of Ancona, Italy
    J Antimicrob Chemother 51:843-7. 2003
    ..SMAP-29 exerted the highest activity against both sporozoites and oocysts...
  27. ncbi request reprint Neutralization of endotoxin in vitro and in vivo by Bac7(1-35), a proline-rich antibacterial peptide
    Roberto Ghiselli
    Department of General Surgery, Istituto Nazionale Riposo e Cura Anziani, University of Ancona, Ancona, Italy
    Shock 19:577-81. 2003
    ..These results provide evidence for the ability of Bac7(1-35) to effectively bind LPS and protect animals from lethal effects of this molecule, and point to its potential use for the treatment of endotoxin-induced septic shock...
  28. ncbi request reprint Production of a recombinant antimicrobial peptide in transgenic plants using a modified VMA intein expression system
    Carla Morassutti
    DPVTA, Universita di Udine, Via delle Scienze 208, Udine, Italy
    FEBS Lett 519:141-6. 2002
    ..These results indicate that a modified intein expression system can be used to produce pharmaceutical peptides in transgenic plants...
  29. ncbi request reprint Cathelicidin peptide sheep myeloid antimicrobial peptide-29 prevents endotoxin-induced mortality in rat models of septic shock
    Andrea Giacometti
    Institute of Infectious Diseases and Public Health, I N R C A I R R C S, Universita Politecnica delle Marche, Ancona, Italy
    Am J Respir Crit Care Med 169:187-94. 2004
    ..No statistically significant differences were noted between SMAP-29 and polymyxin B. SMAP-29, because of its double antiendotoxin and antimicrobial activities, could be an interesting compound for septic shock treatment...