M Zaccolo

Summary

Affiliation: University of Padova
Country: Italy

Publications

  1. pmc Missense mutations in desmocollin-2 N-terminus, associated with arrhythmogenic right ventricular cardiomyopathy, affect intracellular localization of desmocollin-2 in vitro
    Giorgia Beffagna
    Department of Biology, University of Padua, Padua, Italy
    BMC Med Genet 8:65. 2007
  2. ncbi request reprint cAMP and cGMP signaling cross-talk: role of phosphodiesterases and implications for cardiac pathophysiology
    Manuela Zaccolo
    Dulbecco Telethon Institute, Venetian Institute for Molecular Medicine, Padova, Italy
    Circ Res 100:1569-78. 2007
  3. ncbi request reprint Use of chimeric fluorescent proteins and fluorescence resonance energy transfer to monitor cellular responses
    Manuela Zaccolo
    Dulbecco Telethon Institute, Venetian Institute of Molecular Medicine, Via Orus 2, 35129 Padova, Italy
    Circ Res 94:866-73. 2004
  4. ncbi request reprint Imaging the cAMP-dependent signal transduction pathway
    M Zaccolo
    Dulbecco Telethon Institute at the Venetian Institute of Molecular Medicine, Via Orus 2, 35100 Padova, Italy
    Biochem Soc Trans 33:1323-6. 2005
  5. ncbi request reprint CAMP and Ca2+ interplay: a matter of oscillation patterns
    Manuela Zaccolo
    Dulbecco Telethon Institute, Venetian Institute of Molecular Medicine, Via Orus 2, Padua, Italy
    Trends Neurosci 26:53-5. 2003
  6. ncbi request reprint Phosphodiesterases and compartmentalized cAMP signalling in the heart
    Manuela Zaccolo
    Dulbecco Telethon Institute, Venetian Institute of Molecular Medicine, Via Orus 2, I 35124 Padova, Italy
    Eur J Cell Biol 85:693-7. 2006
  7. ncbi request reprint Restricted diffusion of a freely diffusible second messenger: mechanisms underlying compartmentalized cAMP signalling
    M Zaccolo
    Dulbecco Telethon Institute, Venetian Institute of Molecular Medicine, Via Orus 2, 35100 Padova, Italy
    Biochem Soc Trans 34:495-7. 2006
  8. ncbi request reprint Discrete microdomains with high concentration of cAMP in stimulated rat neonatal cardiac myocytes
    Manuela Zaccolo
    Department of Biomedical Sciences and Venetian Institute for Molecular Medicine, University of Padua, Via Orus 2, 35129 Padua, Italy
    Science 295:1711-5. 2002
  9. ncbi request reprint Imaging signal transduction in living cells with GFP-based probes
    M Zaccolo
    Dipartimento di Scienze Biomediche Sperimentali and Centro CNR per le Biomembrane, University of Padova, Italy
    IUBMB Life 49:375-9. 2000
  10. ncbi request reprint Heterogeneity of second messenger levels in living cells
    M Zaccolo
    Department of Biomedical Sciences, CNR Centre of Biomembranes, University of Padua, Italy
    Novartis Found Symp 239:85-93; discussion 93-5, 150-9. 2001

Collaborators

Detail Information

Publications43

  1. pmc Missense mutations in desmocollin-2 N-terminus, associated with arrhythmogenic right ventricular cardiomyopathy, affect intracellular localization of desmocollin-2 in vitro
    Giorgia Beffagna
    Department of Biology, University of Padua, Padua, Italy
    BMC Med Genet 8:65. 2007
    ..We screened 54 ARVC probands for mutations in desmocollin-2 (DSC2), the only desmocollin isoform expressed in cardiac tissue...
  2. ncbi request reprint cAMP and cGMP signaling cross-talk: role of phosphodiesterases and implications for cardiac pathophysiology
    Manuela Zaccolo
    Dulbecco Telethon Institute, Venetian Institute for Molecular Medicine, Padova, Italy
    Circ Res 100:1569-78. 2007
    ..Gaining a more precise understanding of how these cross-talk mechanisms are individually regulated and coordinated is an important direction for future research...
  3. ncbi request reprint Use of chimeric fluorescent proteins and fluorescence resonance energy transfer to monitor cellular responses
    Manuela Zaccolo
    Dulbecco Telethon Institute, Venetian Institute of Molecular Medicine, Via Orus 2, 35129 Padova, Italy
    Circ Res 94:866-73. 2004
    ....
  4. ncbi request reprint Imaging the cAMP-dependent signal transduction pathway
    M Zaccolo
    Dulbecco Telethon Institute at the Venetian Institute of Molecular Medicine, Via Orus 2, 35100 Padova, Italy
    Biochem Soc Trans 33:1323-6. 2005
    ....
  5. ncbi request reprint CAMP and Ca2+ interplay: a matter of oscillation patterns
    Manuela Zaccolo
    Dulbecco Telethon Institute, Venetian Institute of Molecular Medicine, Via Orus 2, Padua, Italy
    Trends Neurosci 26:53-5. 2003
    ..Recent work points to interdependent Ca(2+) and cAMP oscillation patterns as a new paradigm for signal transduction...
  6. ncbi request reprint Phosphodiesterases and compartmentalized cAMP signalling in the heart
    Manuela Zaccolo
    Dulbecco Telethon Institute, Venetian Institute of Molecular Medicine, Via Orus 2, I 35124 Padova, Italy
    Eur J Cell Biol 85:693-7. 2006
    ....
  7. ncbi request reprint Restricted diffusion of a freely diffusible second messenger: mechanisms underlying compartmentalized cAMP signalling
    M Zaccolo
    Dulbecco Telethon Institute, Venetian Institute of Molecular Medicine, Via Orus 2, 35100 Padova, Italy
    Biochem Soc Trans 34:495-7. 2006
    ..In this presentation, the molecular and structural mechanisms responsible for compartmentalized PKA signalling and restricted diffusion of cAMP will be discussed...
  8. ncbi request reprint Discrete microdomains with high concentration of cAMP in stimulated rat neonatal cardiac myocytes
    Manuela Zaccolo
    Department of Biomedical Sciences and Venetian Institute for Molecular Medicine, University of Padua, Via Orus 2, 35129 Padua, Italy
    Science 295:1711-5. 2002
    ..Furthermore, we demonstrate that such gradients of cAMP specifically activate a subset of protein kinase A molecules anchored in proximity to the T tubule...
  9. ncbi request reprint Imaging signal transduction in living cells with GFP-based probes
    M Zaccolo
    Dipartimento di Scienze Biomediche Sperimentali and Centro CNR per le Biomembrane, University of Padova, Italy
    IUBMB Life 49:375-9. 2000
    ..These genetically manipulated GFPs allow monitoring of the biochemistry of the cell in real time and thus offer the possibility to gain a more precise view of the functioning of live cells...
  10. ncbi request reprint Heterogeneity of second messenger levels in living cells
    M Zaccolo
    Department of Biomedical Sciences, CNR Centre of Biomembranes, University of Padua, Italy
    Novartis Found Symp 239:85-93; discussion 93-5, 150-9. 2001
    ..We also demonstrate that by using such cAMP sensor, localized increase of this second messenger can be observed upon selective stimulation of plasma membrane receptors...
  11. ncbi request reprint A complex phosphodiesterase system controls beta-adrenoceptor signalling in cardiomyocytes
    M Mongillo
    Dulbecco Telethon Institute, Venetian Institute of Molecular Medicine, Via Orus 2, 35100 Padova, Italy
    Biochem Soc Trans 34:510-1. 2006
    ..Each PDE family is characterized by unique functional properties and contributes to a cAMP-degrading system enabling the modulation of PKA activity in a stimulus-dependent fashion...
  12. doi request reprint Space and time-resolved gene expression experiments on cultured mammalian cells by a single-cell electroporation microarray
    S Vassanelli
    University of Padova, Department of Human Anatomy and Physiology, Section of Physiology, via Marzolo 3 35131, Padova, Italy
    N Biotechnol 25:55-67. 2008
    ..We focused on DNA oligonucleotides (ODNs), short interfering RNAs (siRNAs), and DNA plasmid vectors, thus providing a versatile and easy-to-use platform for time-resolved gene expression experiments in single mammalian cells...
  13. doi request reprint Protein kinase A type I and type II define distinct intracellular signaling compartments
    Giulietta Di Benedetto
    Dulbecco Telethon Institute, Venetian Institute of Molecular Medicine, Padova, Italy
    Circ Res 103:836-44. 2008
    ..The selective activation of individual PKA isoforms thus leads to phosphorylation of unique subsets of downstream targets...
  14. pmc Biochemical characterization and cellular imaging of a novel, membrane permeable fluorescent cAMP analog
    Daniela Moll
    University of Kassel, Department of Biochemistry, Heinrich Plett Strasse 40, 34132 Kassel, Germany
    BMC Biochem 9:18. 2008
    ....
  15. ncbi request reprint Compartmentalized cAMP/PKA signalling regulates cardiac excitation-contraction coupling
    Valentina Lissandron
    Dulbecco Telethon Institute, Venetian Institute of Molecular Medicine, Via Orus 2, 35100, Padova, Italy
    J Muscle Res Cell Motil 27:399-403. 2006
    ..Here we discuss what are the molecular mechanisms leading to selective modulation of ECC in cardiac myocytes with a particular focus on the role of spatial confinement of PKA subsets and the compartmentalization of cAMP...
  16. ncbi request reprint Real-time analysis of cAMP-mediated regulation of ciliary motility in single primary human airway epithelial cells
    Andreas Schmid
    Division of Pulmonary and Critical Care Medicine, University of Miami School of Medicine, 1600 NW 10th Avenue, RMSB 7063, Miami, FL 33136, USA
    J Cell Sci 119:4176-86. 2006
    ....
  17. pmc Cell entry and cAMP imaging of anthrax edema toxin
    Federica Dal Molin
    Dipartimento di Scienze Biomediche ed Istituto CNR Neuroscienze, Padova, Italy
    EMBO J 25:5405-13. 2006
    ..EF remains associated to these compartments and its activity shows a perinuclear localization generating intracellular cAMP concentration gradients from the cell centre to the periphery...
  18. pmc AKAP complex regulates Ca2+ re-uptake into heart sarcoplasmic reticulum
    Birgitte Lygren
    Biotechnology Centre of Oslo, University of Oslo, PO Box 1125, Blindern, N 0317 Oslo, Norway
    EMBO Rep 8:1061-7. 2007
    ..This prevents the subsequent release of PLN from SERCA2, thereby affecting the Ca(2+) re-uptake into the sarcoplasmic reticulum induced by adrenergic stimuli...
  19. pmc PGE(1) stimulation of HEK293 cells generates multiple contiguous domains with different [cAMP]: role of compartmentalized phosphodiesterases
    Anna Terrin
    Dulbecco Telethon Institute, Venetian Institute of Molecular Medicine, 35129 Padova, Italy
    J Cell Biol 175:441-51. 2006
    ....
  20. pmc Nitroxyl improves cellular heart function by directly enhancing cardiac sarcoplasmic reticulum Ca2+ cycling
    Carlo G Tocchetti
    Cardiology Division, Johns Hopkins Medical Institutions, 720 Rutland Ave, Baltimore, MD 21205, USA
    Circ Res 100:96-104. 2007
    ..Rather, the data support HNO/thiolate interactions that enhance the activity of intracellular Ca(2+) cycling proteins. These findings suggest HNO donors are attractive candidates for the pharmacological treatment of heart failure...
  21. pmc Imaging of cAMP levels and protein kinase A activity reveals that retinal waves drive oscillations in second-messenger cascades
    Timothy A Dunn
    Neurobiology Section, Division of Biological Sciences, University of California, San Diego, La Jolla, California 92093, USA
    J Neurosci 26:12807-15. 2006
    ..These results show a link between neural activity and an intracellular biochemical cascade associated with plasticity, axon guidance, and neural differentiation...
  22. ncbi request reprint Unitary permeability of gap junction channels to second messengers measured by FRET microscopy
    Victor H Hernandez
    Istituto Veneto di Medicina Molecolare, Fondazione per la Ricerca Biomedica Avanzata, 35129 Padova, Italy
    Nat Methods 4:353-8. 2007
    ..This method permits quantification of defects of metabolic coupling and can be used to investigate interdependence of intercellular diffusion and cross-talk between diverse signaling pathways...
  23. doi request reprint cAMP imaging of cells treated with pertussis toxin, cholera toxin, and anthrax edema toxin
    Federica Dal Molin
    Dipartimento di Scienze Biomediche and Istituto C N R Neuroscienze, Universita di Padova, Viale G Colombo n 3, 35121 Padova, Italy
    Biochem Biophys Res Commun 376:429-33. 2008
    ..On the contrary, CT, which acts on the plasma membrane adenylate cyclase, did not. The potential of imaging methods in studying the mode of entry and the intracellular action of bacterial toxins is discussed...
  24. pmc Spatiotemporal coupling of cAMP transporter to CFTR chloride channel function in the gut epithelia
    Chunying Li
    Department of Physiology, The University of Tennessee Health Science Center, 894 Union Avenue, 420 Nash, Memphis, TN 38163, USA
    Cell 131:940-51. 2007
    ..Mrp4 knockout mice are more prone to CFTR-mediated secretory diarrhea. Our findings have important implications for disorders such as inflammatory bowel disease and secretory diarrhea...
  25. ncbi request reprint Transgenic fruit-flies expressing a FRET-based sensor for in vivo imaging of cAMP dynamics
    Valentina Lissandron
    Dulbecco Telethon Institute at the Venetian Institute of Molecular Medicine, Via Orus 2, Padova, Italy
    Cell Signal 19:2296-303. 2007
    ..These transgenic animals represent a novel tool for understanding the physiology of the cAMP signalling pathway in the context of a functioning body...
  26. ncbi request reprint Compartmentalized phosphodiesterase-2 activity blunts beta-adrenergic cardiac inotropy via an NO/cGMP-dependent pathway
    Marco Mongillo
    Dulbecco Telethon Institute, Venetian Institute of Molecular Medicine, Padova, Italy
    Circ Res 98:226-34. 2006
    ..Our study illustrates the key role of compartmentalized PDE2 in the control of catecholamine-generated cAMP and furthers our understanding of localized cAMP signaling...
  27. ncbi request reprint Compartmentalisation of cAMP and Ca(2+) signals
    Manuela Zaccolo
    Venetian Institute of Molecular Medicine, Via Orus 2, 35121 Padua, Italy
    Curr Opin Cell Biol 14:160-6. 2002
    ..The development of recombinant probes based on the green fluorescent protein have allowed the monitoring of these second messenger levels in single cells, with high spatial and temporal resolution...
  28. ncbi request reprint Measuring dynamic changes in cAMP using fluorescence resonance energy transfer
    Sandrine Evellin
    Venetian Institute for Molecular Medicine, Dulbecco Telethon Institute, Padova, Italy
    Methods Mol Biol 284:259-70. 2004
    ..This method allows the study of cAMP fluctuations in living cells with high resolution both in time and in space...
  29. ncbi request reprint Fluorescence resonance energy transfer-based analysis of cAMP dynamics in live neonatal rat cardiac myocytes reveals distinct functions of compartmentalized phosphodiesterases
    Marco Mongillo
    Dulbecco Telethon Institute, Padova, Italy
    Circ Res 95:67-75. 2004
    ..PDE3 and PDE4 localize to distinct compartments and this may underpin their different functional roles. Our findings indicate the importance of distinctly localized PDE isoenzymes in determining compartmentalized cAMP signaling...
  30. ncbi request reprint TCR- and CD28-mediated recruitment of phosphodiesterase 4 to lipid rafts potentiates TCR signaling
    Hilde Abrahamsen
    Biotechnology Centre, University of Oslo, Norway
    J Immunol 173:4847-58. 2004
    ..The specific recruitment of PDE4 thus serves to abrogate the negative feedback by cAMP which is elicited in the absence of a coreceptor stimulus...
  31. pmc Detecting cAMP-induced Epac activation by fluorescence resonance energy transfer: Epac as a novel cAMP indicator
    Bas Ponsioen
    Division of Cell Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066CX Amsterdam, The Netherlands
    EMBO Rep 5:1176-80. 2004
    ..These properties make Epac-based FRET probes the preferred indicators for monitoring cAMP levels in vivo...
  32. ncbi request reprint cGMP catabolism by phosphodiesterase 5A regulates cardiac adrenergic stimulation by NOS3-dependent mechanism
    Eiki Takimoto
    Division of Cardiology, Department of Medicine, Johns Hopkins Medical Institutions, Baltimore, MD, USA
    Circ Res 96:100-9. 2005
    ....
  33. pmc Termination of cAMP signals by Ca2+ and G(alpha)i via extracellular Ca2+ sensors: a link to intracellular Ca2+ oscillations
    Andrea Gerbino
    Veterans Affairs Boston Healthcare System, West Roxbury, MA 02132, USA
    J Cell Biol 171:303-12. 2005
    ..Our data suggest that the cAMP machinery in HEK cells acts as a low-pass filter disregarding the relatively rapid Ca2+ spiking stimulated by Ca(2+)-mobilizing agonists under physiological conditions...
  34. ncbi request reprint Beta-adrenergic- and muscarinic receptor-induced changes in cAMP activity in adult cardiac myocytes detected with FRET-based biosensor
    Sunita Warrier
    Department of Physiology and Biophysics, Case Western Reserve University, Cleveland, OH 44106 4970, USA
    Am J Physiol Cell Physiol 289:C455-61. 2005
    ..The adenovirus-based approach we have developed should facilitate the use of this biosensor in studying cAMP and PKA-dependent signaling mechanisms in a wide variety of cell types...
  35. pmc Protein kinase A gating of a pseudopodial-located RhoA/ROCK/p38/NHE1 signal module regulates invasion in breast cancer cell lines
    Rosa A Cardone
    Department of General and Environmental Physiology, University of Bari, 70126 Bari, Italy
    Mol Biol Cell 16:3117-27. 2005
    ....
  36. ncbi request reprint Molecular basis of the allosteric mechanism of cAMP in the regulatory PKA subunit
    Sergio Pantano
    International School for Advanced Studies ISAS and INFM DEMOCRITOS Modeling Center for Research in Atomistic Simulation, Trieste, Italy
    FEBS Lett 579:2679-85. 2005
    ..In addition, our calculations provide a rationale for the experimentally observed cAMP selective binding to PKA...
  37. ncbi request reprint Study of cyclic adenosine monophosphate microdomains in cells
    Marco Mongillo
    Dulbecco Telethon Institute and Venetian Institute of Molecular Medicine, Padova, Italy
    Methods Mol Biol 307:1-13. 2005
    ..This chapter describes the molecular basis on which such a genetically encoded cAMP sensor relies and the tools and methods required to perform cAMP measurements in living samples...
  38. ncbi request reprint Improvement of a FRET-based indicator for cAMP by linker design and stabilization of donor-acceptor interaction
    Valentina Lissandron
    Dulbecco Telethon Institute at Venetian Institute of Molecular Medicine, Via Orus 2, 35129 Padova, Italy
    J Mol Biol 354:546-55. 2005
    ..The resulting linker L20 displayed lower flexibility and higher helical content than L22. When inserted in the cAMP indicator, L20 yielded a probe showing almost doubled FRET efficiency and a substantially improved dynamic range...
  39. ncbi request reprint Selection of functional antibodies on the basis of valency
    Manuela Zaccolo
    Dipartimento di Scienze Biomediche Sperimentali, Universita di Padova, Padova, Italy
    Methods Mol Biol 178:255-8. 2002
  40. ncbi request reprint Human MYO18B, a novel unconventional myosin heavy chain expressed in striated muscles moves into the myonuclei upon differentiation
    Michela Salamon
    CRIBI Biotechnology Center, Universita degli Studi di Padova, Via Ugo Bassi 58 B, 35131, Padova, Italy
    J Mol Biol 326:137-49. 2003
    ..In some cases, cardiomyocytes show a partial sarcomeric pattern of MYO18B alternating that of alpha-actinin-2. In skeletal muscle the cytoplasmic MYO18B results much more evident in the fast type fibers...
  41. doi request reprint Developmentally acquired PKA localisation in mouse oocytes and embryos
    Rachel J Webb
    Department of Physiology, University College London, Gower Street, London, WC1E 6BT, UK
    Dev Biol 317:36-45. 2008
    ..RT-PCR and Western blotting revealed two candidate AKAPs that are known to be targeted to mitochondria, AKAP1 and D-AKAP2. In summary these data show a dynamic regulation of PKA localisation during oocyte and early embryo development...
  42. ncbi request reprint Photon moment analysis in cells in the presence of photo-bleaching
    Michele Caccia
    Department of Physics, University of Milan Bicocca, Milan, Italy
    Appl Spectrosc 59:227-36. 2005
    ....
  43. doi request reprint Beta-oestradiol rescues DeltaF508CFTR functional expression in human cystic fibrosis airway CFBE41o- cells through the up-regulation of NHERF1
    Teresa Fanelli
    Department of General and Environmental Physiology, University of Bari, Via Amendola 165 A, Bari 70126, Italy
    Biol Cell 100:399-412. 2008
    ....