Carla Borri Voltattorni

Summary

Affiliation: University of Verona
Country: Italy

Publications

  1. ncbi request reprint Behavior of fluorinated analogs of L-(3,4-dihydroxyphenyl)alanine and L-threo-(3,4-dihydroxyphenyl)serine as substrates for Dopa decarboxylase
    Carla Borri Voltattorni
    Dipartimento di Scienze Neurologiche e della Visione, Sezione di Chimica Biologica, Facolta di Medicina e Chirurgia, Universita degli Studi di Verona, Verona, Italy
    Biochem Biophys Res Commun 295:107-11. 2002
  2. ncbi request reprint Treponema denticola cystalysin catalyzes beta-desulfination of L-cysteine sulfinic acid and beta-decarboxylation of L-aspartate and oxalacetate
    Barbara Cellini
    Dipartimento di Scienze Neurologiche e della Visione, Sezione di Chimica Biologica, Facolta di Medicina e Chirurgia, Universita degli Studi di Verona, Strada Le Grazie, 8, 37134 Verona, Italy
    FEBS Lett 554:306-10. 2003
  3. pmc Molecular Insight into the Synergism between the Minor Allele of Human Liver Peroxisomal Alanine:Glyoxylate Aminotransferase and the F152I Mutation
    Barbara Cellini
    Dipartimento di Scienze Morfologico Biomediche, Sezione di Chimica Biologica, Facolta di Medicina e Chirurgia, Universita degli Studi di Verona, Strada Le Grazie, 8, 37134 Verona, Italy
    J Biol Chem 284:8349-58. 2009
  4. doi request reprint Multiple roles of the active site lysine of Dopa decarboxylase
    Mariarita Bertoldi
    Dipartimento di Scienze Morfologico Biomediche, Sezione di Chimica Biologica, Facolta di Medicina e Chirurgia, Universita di Verona, 37134 Verona, Italy
    Arch Biochem Biophys 488:130-9. 2009
  5. doi request reprint An engineered folded PLP-bound monomer of Treponema denticola cystalysin reveals the effect of the dimeric structure on the catalytic properties of the enzyme
    Riccardo Montioli
    Dipartimento di Scienze Morfologico Biomediche, Sezione di Chimica Biologica, Facolta di Medicina e Chirurgia, Universita degli Studi di Verona, Strada Le Grazie 8, 37134 Verona, Italy
    Proteins 74:304-17. 2009
  6. doi request reprint Human liver peroxisomal alanine:glyoxylate aminotransferase: Different stability under chemical stress of the major allele, the minor allele, and its pathogenic G170R variant
    Barbara Cellini
    Dipartimento di Scienze della Vita e della Riproduzione, Sezione di Chimica Biologica, Facolta di Medicina e Chirurgia, Universita degli Studi di Verona, Strada Le Grazie, 8 37134 Verona, Italy
    Biochimie 92:1801-11. 2010
  7. ncbi request reprint Site-directed mutagenesis provides insight into racemization and transamination of alanine catalyzed by Treponema denticola cystalysin
    Barbara Cellini
    Dipartimento di Scienze Neurologiche e della Visione, Sezione di Chimica Biologica, Facolta di Medicina e Chirurgia, Universita degli Studi di Verona, Strada Le Grazie 8, 37134 Verona, Italy
    J Biol Chem 279:36898-905. 2004
  8. pmc Molecular defects of the glycine 41 variants of alanine glyoxylate aminotransferase associated with primary hyperoxaluria type I
    Barbara Cellini
    Dipartimento di Scienze Morfologico Biomediche, Sezione di Chimica Biologica, Facolta di Medicina e Chirurgia, Universita degli Studi di Verona, Strada Le Grazie, 8, 37134 Verona, Italy
    Proc Natl Acad Sci U S A 107:2896-901. 2010
  9. ncbi request reprint Dimerization and folding processes of Treponema denticola cystalysin: the role of pyridoxal 5'-phosphate
    Barbara Cellini
    Dipartimento di Scienze Morfologico Biomediche, Sezione di Chimica Biologica, Facolta di Medicina e Chirurgia, Universita degli Studi di Verona, Strada Le Grazie, 8, 37134 Verona, Italy
    Biochemistry 45:14140-54. 2006
  10. pmc Interaction of human Dopa decarboxylase with L-Dopa: spectroscopic and kinetic studies as a function of pH
    Riccardo Montioli
    Section of Biological Chemistry, Department of Life Sciences and Reproduction, University of Verona, Verona, Italy
    Biomed Res Int 2013:161456. 2013

Collaborators

Detail Information

Publications26

  1. ncbi request reprint Behavior of fluorinated analogs of L-(3,4-dihydroxyphenyl)alanine and L-threo-(3,4-dihydroxyphenyl)serine as substrates for Dopa decarboxylase
    Carla Borri Voltattorni
    Dipartimento di Scienze Neurologiche e della Visione, Sezione di Chimica Biologica, Facolta di Medicina e Chirurgia, Universita degli Studi di Verona, Verona, Italy
    Biochem Biophys Res Commun 295:107-11. 2002
    ..Condensation of fluorinated catecholamines and catechol amino acids with endogenous aldehydes will be investigated as an approach to study possible mechanisms of L-Dopa-linked neurotoxicity...
  2. ncbi request reprint Treponema denticola cystalysin catalyzes beta-desulfination of L-cysteine sulfinic acid and beta-decarboxylation of L-aspartate and oxalacetate
    Barbara Cellini
    Dipartimento di Scienze Neurologiche e della Visione, Sezione di Chimica Biologica, Facolta di Medicina e Chirurgia, Universita degli Studi di Verona, Strada Le Grazie, 8, 37134 Verona, Italy
    FEBS Lett 554:306-10. 2003
    ..The k(cat) and K(m) of this reaction are 0.15+/-0.01 s(-1) and 13+/-2 mM, respectively. Possible mechanistic and physiological implications are discussed...
  3. pmc Molecular Insight into the Synergism between the Minor Allele of Human Liver Peroxisomal Alanine:Glyoxylate Aminotransferase and the F152I Mutation
    Barbara Cellini
    Dipartimento di Scienze Morfologico Biomediche, Sezione di Chimica Biologica, Facolta di Medicina e Chirurgia, Universita degli Studi di Verona, Strada Le Grazie, 8, 37134 Verona, Italy
    J Biol Chem 284:8349-58. 2009
    ....
  4. doi request reprint Multiple roles of the active site lysine of Dopa decarboxylase
    Mariarita Bertoldi
    Dipartimento di Scienze Morfologico Biomediche, Sezione di Chimica Biologica, Facolta di Medicina e Chirurgia, Universita di Verona, 37134 Verona, Italy
    Arch Biochem Biophys 488:130-9. 2009
    ..Kinetic analyses reveal that Lys303 is involved in external aldimine formation and hydrolysis as well as in product release which affects the rate-determining step of decarboxylation...
  5. doi request reprint An engineered folded PLP-bound monomer of Treponema denticola cystalysin reveals the effect of the dimeric structure on the catalytic properties of the enzyme
    Riccardo Montioli
    Dipartimento di Scienze Morfologico Biomediche, Sezione di Chimica Biologica, Facolta di Medicina e Chirurgia, Universita degli Studi di Verona, Strada Le Grazie 8, 37134 Verona, Italy
    Proteins 74:304-17. 2009
    ..The structural basis of the stereospecific transaminase activity displayed by the engineered folded PLP-bound monomer has been analyzed and discussed...
  6. doi request reprint Human liver peroxisomal alanine:glyoxylate aminotransferase: Different stability under chemical stress of the major allele, the minor allele, and its pathogenic G170R variant
    Barbara Cellini
    Dipartimento di Scienze della Vita e della Riproduzione, Sezione di Chimica Biologica, Facolta di Medicina e Chirurgia, Universita degli Studi di Verona, Strada Le Grazie, 8 37134 Verona, Italy
    Biochimie 92:1801-11. 2010
    ..1 M urea. These biochemical results are discussed in the light of the characteristics of the enzymatic phenotype of PH1 patients bearing G170R mutation in AGT-Mi and the positive response of these patients to pyridoxine treatment...
  7. ncbi request reprint Site-directed mutagenesis provides insight into racemization and transamination of alanine catalyzed by Treponema denticola cystalysin
    Barbara Cellini
    Dipartimento di Scienze Neurologiche e della Visione, Sezione di Chimica Biologica, Facolta di Medicina e Chirurgia, Universita degli Studi di Verona, Strada Le Grazie 8, 37134 Verona, Italy
    J Biol Chem 279:36898-905. 2004
    ..Moreover, this study highlights the importance of the interaction of Tyr-123 with water molecules for efficient proton abstraction/donation function on the re face...
  8. pmc Molecular defects of the glycine 41 variants of alanine glyoxylate aminotransferase associated with primary hyperoxaluria type I
    Barbara Cellini
    Dipartimento di Scienze Morfologico Biomediche, Sezione di Chimica Biologica, Facolta di Medicina e Chirurgia, Universita degli Studi di Verona, Strada Le Grazie, 8, 37134 Verona, Italy
    Proc Natl Acad Sci U S A 107:2896-901. 2010
    ..The detailed insight into how G41 mutations act on the structure-function of AGT may contribute to achieve the ultimate goal of correcting the effects of these mutations...
  9. ncbi request reprint Dimerization and folding processes of Treponema denticola cystalysin: the role of pyridoxal 5'-phosphate
    Barbara Cellini
    Dipartimento di Scienze Morfologico Biomediche, Sezione di Chimica Biologica, Facolta di Medicina e Chirurgia, Universita degli Studi di Verona, Strada Le Grazie, 8, 37134 Verona, Italy
    Biochemistry 45:14140-54. 2006
    ..The dimerization and folding processes of cystalysin have been compared with those of another PLP C-S lyase, MalY from E. coli, and the possible relevance of their PLP binding mode in these processes has been discussed...
  10. pmc Interaction of human Dopa decarboxylase with L-Dopa: spectroscopic and kinetic studies as a function of pH
    Riccardo Montioli
    Section of Biological Chemistry, Department of Life Sciences and Reproduction, University of Verona, Verona, Italy
    Biomed Res Int 2013:161456. 2013
    ..This suggests that this pK represents the ESH(+) → ES catalytic step. The assignment of the pKs of 7.9 and 8.3 observed on the basic side of k(cat) and the PLP binding affinity profiles, respectively, is also analyzed and discussed...
  11. ncbi request reprint Holo- and apo-cystalysin from Treponema denticola: two different conformations
    Barbara Cellini
    Dipartimento di Scienze Morfologico Biomediche, Sezione di Chimica Biologica, Facolta di Medicina e Chirurgia, Strada Le Grazie 8, Universita di Verona, 37134 Verona, Italy
    Arch Biochem Biophys 455:31-9. 2006
    ..Although the break causes the loss of lyase, racemase and transaminase activities of D-alanine, it does not abolish the transaminase activity of L-alanine. Possible mechanistic and physiological implications are proposed...
  12. ncbi request reprint Construction, purification and characterization of untagged human liver alanine-glyoxylate aminotransferase expressed in Escherichia coli
    Barbara Cellini
    Dipartimento di Scienze Morfologico Biomediche, Universita degli Studi di Verona, Strada Le Grazie, 8, 37134 Verona, Italy
    Protein Pept Lett 15:153-9. 2008
    ..This is prerequisite to understand how pathological mutations affect the proper native quaternary and tertiary structure, stability, and targeting of the enzyme...
  13. doi request reprint Human liver peroxisomal alanine:glyoxylate aminotransferase: characterization of the two allelic forms and their pathogenic variants
    Barbara Cellini
    Dipartimento di Scienze della Vita e della Riproduzione, Sezione di Chimica Biologica, Facolta di Medicina e Chirurgia, Universita degli Studi di Verona, Strada Le Grazie, 8 37134 Verona, Italy
    Biochim Biophys Acta 1814:1577-84. 2011
    ..This article is part of a Special Issue entitled: Pyridoxal Phospate Enzymology...
  14. doi request reprint S250F variant associated with aromatic amino acid decarboxylase deficiency: molecular defects and intracellular rescue by pyridoxine
    Riccardo Montioli
    Department of Life Sciences and Reproduction, University of Verona, Verona, Italy
    Hum Mol Genet 22:1615-24. 2013
    ..This strongly suggests that 4-PBA and/or pyridoxine administration may be of important value in therapy of patients bearing the S250F mutation...
  15. ncbi request reprint Spectroscopic and kinetic analyses reveal the pyridoxal 5'-phosphate binding mode and the catalytic features of Treponema denticola cystalysin
    Mariarita Bertoldi
    Dipartimento di Scienze Neurologiche e della Visione, Sezione di Chimica Biologica, Facolta di Medicina e Chirurgia, Universita degli Studi di Verona, Strada Le Grazie, 8, 37134 Verona, Italy
    Biochemistry 41:9153-64. 2002
    ..In addition, reinvestigation of the substrate spectrum of alpha,beta-elimination indicates that cystalysin is a cyst(e)ine C-S lyase rather than a cysteine desulfhydrase as claimed previously...
  16. ncbi request reprint A quinonoid is an intermediate of oxidative deamination reaction catalyzed by Dopa decarboxylase
    Mariarita Bertoldi
    Dipartimento di Scienze Neurologiche e della Visione, Sezione di Chimica Biologica, Universita di Verona, Italy
    FEBS Lett 579:5175-80. 2005
    ..All these data, besides validating the functional DDC active site model, represent an important step toward the elucidation of the catalytic pathway of oxidative deamination...
  17. pmc Crystal structure of the S187F variant of human liver alanine: aminotransferase associated with primary hyperoxaluria type I and its functional implications
    Elisa Oppici
    Department of Life Sciences and Reproduction, Section of Biological Chemistry, University of Verona, Verona, Italy
    Proteins 81:1457-65. 2013
    ..Proposals for the treatment of patients bearing S187F mutation are discussed on the basis of these results...
  18. ncbi request reprint Mutation of tyrosine 332 to phenylalanine converts dopa decarboxylase into a decarboxylation-dependent oxidative deaminase
    Mariarita Bertoldi
    Dipartimento di Scienze Neurologiche e della Visione, Sezione di Chimica Biologica, Facolta di Medicina e Chirurgia, Universita degli Studi di Verona, Strada Le Grazie, 8, 37134 Verona, Italy
    J Biol Chem 277:36357-62. 2002
    ..However, for all the mutant-catalyzed reactions, an increase in K(m) values is observed, suggesting that Y --> F replacement also affects substrate binding...
  19. ncbi request reprint Probing the role of Tyr 64 of Treponema denticola cystalysin by site-directed mutagenesis and kinetic studies
    Barbara Cellini
    Dipartimento di Scienze Neurologiche e della Visione, Sezione di Chimica Biologica, Facolta di Medicina e Chirurgia, Universita degli Studi di Verona, Strada Le Grazie, 8, 37134 Verona, Italy
    Biochemistry 44:13970-80. 2005
    ..Altogether, these results provide new insights into the catalytic mechanism of cystalysin and highlight the functional role of Tyr 64...
  20. pmc Treponema denticola cystalysin exhibits significant alanine racemase activity accompanied by transamination: mechanistic implications
    Mariarita Bertoldi
    Dipartimento di Scienze Neurologiche e della Visione, Sezione di Chimica Biologica, Facolta di Medicina e Chirurgia, Universita degli Studi di Verona, Strada Le Grazie, 8, 37134 Verona, Italy
    Biochem J 371:473-83. 2003
    ..Together with molecular modelling of the putative binding sites of L- and D-alanine at the active site of the enzyme, the implications of these studies for the mechanisms of the side reactions catalysed by cystalysin are discussed...
  21. ncbi request reprint Reaction and substrate specificity of recombinant pig kidney Dopa decarboxylase under aerobic and anaerobic conditions
    Mariarita Bertoldi
    Dipartimento di Scienze Neurologiche e della Visione, Sezione di Chimica Biologica, Universita degli Studi di Verona, Strada Le Grazie, 8, 37134 Verona, Italy
    Biochim Biophys Acta 1647:42-7. 2003
    ....
  22. ncbi request reprint Lysine 238 is an essential residue for alpha,beta-elimination catalyzed by Treponema denticola cystalysin
    Mariarita Bertoldi
    Dipartimento di Scienze Neurologiche e della Visione, Sezione di Chimica Biologica, Facolta di Medicina e Chirurgia, Universita degli Studi di Verona, Strada Le Grazie, 8, 37134 Verona, Italy
    J Biol Chem 278:37336-43. 2003
    ....
  23. doi request reprint Insights into the mechanism of oxidative deamination catalyzed by DOPA decarboxylase
    Mariarita Bertoldi
    Dipartimento di Scienze Morfologico Biomediche, Sezione di Chimica Biologica, Facolta di Medicina e Chirurgia, Strada Le Grazie, 8, 37134 Verona, Italy
    Biochemistry 47:7187-95. 2008
    ....
  24. doi request reprint Reactions of human liver peroxisomal alanine:glyoxylate aminotransferase with beta-chloro-L-alanine and L-cysteine: spectroscopic and kinetic analysis
    Mariarita Bertoldi
    Dipartimento di Scienze Morfologico Biomediche, Sezione di Chimica Biologica, Facolta di Medicina e Chirurgia, Universita degli Studi di Verona, Strada Le Grazie, 8, 37134 Verona, Italy
    Biochim Biophys Acta 1784:1356-62. 2008
    ..This appears to be consistent with the calculated interaction energies of the L-cysteine, L-alanine and L-serine docked at the active site of AGT...
  25. pmc Folding pathway of the pyridoxal 5'-phosphate C-S lyase MalY from Escherichia coli
    Mariarita Bertoldi
    Dipartimento di Scienze Neurologiche e della Visione, Sezione di Chimica Biologica, Facolta di Medicina e Chirurgia, Universita degli Studi di Verona, Strada Le Grazie, 8, 37134 Verona, Italy
    Biochem J 389:885-98. 2005
    ..Further refolding studies suggest that large misfolded soluble aggregates that cannot be refolded could be responsible for the incomplete re-activation...
  26. pmc Human wild-type alanine:glyoxylate aminotransferase and its naturally occurring G82E variant: functional properties and physiological implications
    Barbara Cellini
    Dipartimento di Scienze Morfologico Biomediche, Sezione di Chimica Biologica, Facolta di Medicina e Chirurgia, Universita degli Studi di Verona, Strada Le Grazie, 8, 37134 Verona, Italy
    Biochem J 408:39-50. 2007
    ..Overall, careful biochemical analyses have allowed elucidation of the mechanism of action of AGT and the way in which the disease causing G82E mutation affects it...