A Scarafoni

Summary

Affiliation: University of Milan
Country: Italy

Publications

  1. ncbi One-step purification of Kunitz soybean trypsin inhibitor
    Marcello Duranti
    Dipartimento di Scienze Molecolari Agroalimentari, Universita di Milano, Via Celoria 2, I 20133 Milan, Italy
    Protein Expr Purif 30:167-70. 2003
  2. ncbi The proteome of exudates from germinating Lupinus albus seeds is secreted through a selective dual-step process and contains proteins involved in plant defence
    Alessio Scarafoni
    Department of Food, Environmental and Nutritional Sciences, Universita degli Studi di Milano, Milano, Italy
    FEBS J 280:1443-59. 2013
  3. ncbi Biochemical and functional characterization of an albumin protein belonging to the hemopexin superfamily from Lens culinaris seeds
    Alessio Scarafoni
    Department of Agrifood Molecular Sciences, Università degli Studi di Milano, Via G Celoria 2, 20133 Milano, Italy
    J Agric Food Chem 59:9637-44. 2011
  4. ncbi Cloning, sequencing and expression in the seeds and radicles of two Lupinus albus conglutin gamma genes
    A Scarafoni
    Dipartmento di Scienze Molecolari Agroalimentari, Universita degli Studi di Milano, Milan, Italy
    Biochim Biophys Acta 1519:147-51. 2001
  5. ncbi Manipulation of the napin primary structure alters its packaging and deposition in transgenic tobacco (Nicotiana tabacum L.) seeds
    A Scarafoni
    Dipartimento di Scienze Molecolari Agroalimentari, Universita degli Studi di Milano, Italy
    Plant Mol Biol 46:727-39. 2001
  6. ncbi gamma-Conglutin, the Lupinus albus XEGIP-like protein, whose expression is elicited by chitosan, lacks of the typical inhibitory activity against GH12 endo-glucanases
    Alessio Scarafoni
    Dipartimento di Scienze Molecolari Agroalimentari, Universita degli Studi di Milano, Via G Celoria 2, 20133 Milano, Italy
    Phytochemistry 71:142-8. 2010
  7. ncbi Identification and characterization of a Bowman-Birk inhibitor active towards trypsin but not chymotrypsin in Lupinus albus seeds
    Alessio Scarafoni
    Department of Agri Food Molecular Sciences, State University of Milan, Via G Celoria 2, 20133 Milano, Italy
    Phytochemistry 69:1820-5. 2008
  8. ncbi Thermal stabilities of lupin seed conglutin gamma protomers and tetramers
    M Duranti
    Dipartimento di Scienze Molecolari Agroalimentari, Universita degli Studi di Milano, Via Celoria 2, I 20133 Milano, Italy
    J Agric Food Chem 48:1118-23. 2000
  9. ncbi Interaction of metal ions with lupin seed conglutin gamma
    M Duranti
    Dipartimento di Scienze Molecolari Agroalimentari, Universita degli Studi di Milano, Italy
    Phytochemistry 56:529-33. 2001
  10. ncbi Inhibitory properties and solution structure of a potent Bowman-Birk protease inhibitor from lentil (Lens culinaris, L) seeds
    Enzio M Ragg
    Department of Agri Food Molecular Sciences, Universita degli Studi, Milano, Italy
    FEBS J 273:4024-39. 2006

Collaborators

Detail Information

Publications16

  1. ncbi One-step purification of Kunitz soybean trypsin inhibitor
    Marcello Duranti
    Dipartimento di Scienze Molecolari Agroalimentari, Universita di Milano, Via Celoria 2, I 20133 Milan, Italy
    Protein Expr Purif 30:167-70. 2003
    ..The yield was greater than that obtained with established procedures. Due to the ease of the procedure proposed, the method is readily scalable to pilot plant or industrial preparations...
  2. ncbi The proteome of exudates from germinating Lupinus albus seeds is secreted through a selective dual-step process and contains proteins involved in plant defence
    Alessio Scarafoni
    Department of Food, Environmental and Nutritional Sciences, Universita degli Studi di Milano, Milano, Italy
    FEBS J 280:1443-59. 2013
    ..The released proteome is involved in protecting the spermosphere environment and thus may act as first defence against pathogens...
  3. ncbi Biochemical and functional characterization of an albumin protein belonging to the hemopexin superfamily from Lens culinaris seeds
    Alessio Scarafoni
    Department of Agrifood Molecular Sciences, Università degli Studi di Milano, Via G Celoria 2, 20133 Milano, Italy
    J Agric Food Chem 59:9637-44. 2011
    ..Denaturation temperature has also been determined. The present work provides new insights about the structural molecular features and the ligand-binding properties and dynamics of this kind of seed albumin...
  4. ncbi Cloning, sequencing and expression in the seeds and radicles of two Lupinus albus conglutin gamma genes
    A Scarafoni
    Dipartmento di Scienze Molecolari Agroalimentari, Universita degli Studi di Milano, Milan, Italy
    Biochim Biophys Acta 1519:147-51. 2001
    ....
  5. ncbi Manipulation of the napin primary structure alters its packaging and deposition in transgenic tobacco (Nicotiana tabacum L.) seeds
    A Scarafoni
    Dipartimento di Scienze Molecolari Agroalimentari, Universita degli Studi di Milano, Italy
    Plant Mol Biol 46:727-39. 2001
    ..Detailed immunolocalisation studies indicate that the insertion of the peptide sequences has a significant effect on the distribution of the napin proteins within the tobacco seed protein bodies...
  6. ncbi gamma-Conglutin, the Lupinus albus XEGIP-like protein, whose expression is elicited by chitosan, lacks of the typical inhibitory activity against GH12 endo-glucanases
    Alessio Scarafoni
    Dipartimento di Scienze Molecolari Agroalimentari, Universita degli Studi di Milano, Via G Celoria 2, 20133 Milano, Italy
    Phytochemistry 71:142-8. 2010
    ....
  7. ncbi Identification and characterization of a Bowman-Birk inhibitor active towards trypsin but not chymotrypsin in Lupinus albus seeds
    Alessio Scarafoni
    Department of Agri Food Molecular Sciences, State University of Milan, Via G Celoria 2, 20133 Milano, Italy
    Phytochemistry 69:1820-5. 2008
    ..The interest in these serine-protease inhibitors arises from the ability to prevent or suppress carcinogen-induced transformation, as shown in various in vitro and in vivo model systems...
  8. ncbi Thermal stabilities of lupin seed conglutin gamma protomers and tetramers
    M Duranti
    Dipartimento di Scienze Molecolari Agroalimentari, Universita degli Studi di Milano, Via Celoria 2, I 20133 Milano, Italy
    J Agric Food Chem 48:1118-23. 2000
    ....
  9. ncbi Interaction of metal ions with lupin seed conglutin gamma
    M Duranti
    Dipartimento di Scienze Molecolari Agroalimentari, Universita degli Studi di Milano, Italy
    Phytochemistry 56:529-33. 2001
    ..A slightly lower binding to immobilised Cu2+ and Co2+ and no binding with Mg2+, Cd2+ and Mn2+ were observed. The role of the numerous histidine residues of conglutin gamma in the binding of Zn2+ is discussed...
  10. ncbi Inhibitory properties and solution structure of a potent Bowman-Birk protease inhibitor from lentil (Lens culinaris, L) seeds
    Enzio M Ragg
    Department of Agri Food Molecular Sciences, Universita degli Studi, Milano, Italy
    FEBS J 273:4024-39. 2006
    ..The inhibitory properties of LCTI, related to the simultaneous presence of two key amino acids (Gln18 and His54), render the molecule unusual within the natural Bowman-Birk inhibitor family...
  11. ncbi Implication of an outer surface lipoprotein in adhesion of Bifidobacterium bifidum to Caco-2 cells
    Simone Guglielmetti
    Department of Food Science and Microbiology, University of Milan, Via Celoria 2, 20133 Milan, Italy
    Appl Environ Microbiol 74:4695-702. 2008
    ..BopA is the first protein described to date to be directly involved in the adhesion of bifidobacteria to Caco-2 cells and to show immunomodulatory activity...
  12. ncbi Metal ions restore the proteolytic resistance of denatured conglutin gamma, a lupin seed glycoprotein, by promoting its refolding
    Marcello Duranti
    Dipartimento di Scienze Molecolari Agroalimentari and Istituto di Fisiologia Veterinaria e Biochimica, Universita degli Studi di Milano, Milano, Italy
    J Agric Food Chem 50:2029-33. 2002
    ..The relevance of these findings for a deeper understanding of the in vivo degradation of plant food proteins and how it is affected by metal ions are discussed...
  13. ncbi Combined 2D electrophoretic approaches for the study of white lupin mature seed storage proteome
    Chiara Magni
    Department of Agrifood Molecular Sciences, State University of Milan, Via Celoria 2, 20133 Milano, Italy
    Phytochemistry 68:997-1007. 2007
    ..The results obtained also extend our knowledge of the seed storage protein polymorphism of white lupin...
  14. ncbi One- and two-dimensional electrophoretic identification of IgE-binding polypeptides of Lupinus albus and other legume seeds
    Chiara Magni
    Department of Agrifood Molecular Sciences, State University of Milan, Via Celoria 2, I-20133 Milan, Italy
    J Agric Food Chem 53:4567-71. 2005
    ..This global electrophoretic approach has allowed the identification of a new potential lupin allergen and confirmed the cross-reactivity among the legume 11S globulin basic subunits...
  15. ncbi Conglutin gamma, a lupin seed protein, binds insulin in vitro and reduces plasma glucose levels of hyperglycemic rats
    Chiara Magni
    Department of Agrifood Molecular Sciences, University of Milan, Milan, Italy
    J Nutr Biochem 15:646-50. 2004
    ..These findings represent the first molecular evidence of the possible use of a legume protein in the control of glycemia...
  16. ncbi Two-dimensional electrophoresis and western-blotting analyses with anti Ara h 3 basic subunit IgG evidence the cross-reacting polypeptides of Arachis hypogaea, Glycine max, and Lupinus albus seed proteomes
    Chiara Magni
    Department of Agrifood Molecular Sciences, University of Milan, Via Celoria 2, 20133 Milan, Italy
    J Agric Food Chem 53:2275-81. 2005
    ..The present findings show the potentiality of 2D electrophoresis in the identification of food allergens and open the way to the traceability of the new cross-reacting proteins in the food chain...