Affiliation: University of Padova
- Beat-to-beat oscillations of mitochondrial [Ca2+] in cardiac cellsV Robert
Department of Biomedical Sciences, CNR Center of Biomembranes, University of Padua, Via Colombo 3, 35121 Padua, Italy
EMBO J 20:4998-5007. 2001..The present data unequivocally demonstrate that in cardiac cells mitochondrial [Ca2+] oscillates synchronously with cytosolic [Ca2+] and that mitochondrial Ca2+ handling rapidly adapts to inotropic or chronotropic inputs...
- The comeback of mitochondria to calcium signallingT Pozzan
Department of Biomedical Sciences and CNR Center for the Study of Biomembranes, University of Padova, Padova, Italy
Cell Calcium 28:279-83. 2000..Particular attention is paid to recent developments which have contributed to a renewed interest in calcium handling by this organelle...
- The renaissance of mitochondrial calcium transportT Pozzan
Department of Biomedical Sciences and CNR Center for the Study of Biomembranes, University of Padova, Italy
Eur J Biochem 267:5269-73. 2000....
- Close contacts with the endoplasmic reticulum as determinants of mitochondrial Ca2+ responsesR Rizzuto
Department of Biomedical Sciences and the National Research Council Center for the Study of Biomembranes, University of Padova, Via Colombo 3, 35121 Padova, Italy
Science 280:1763-6. 1998..These results emphasize the importance of cell architecture and the distribution of organelles in regulation of Ca2+ signaling...
- Delayed activation of the store-operated calcium current induced by calreticulin overexpression in RBL-1 cellsC Fasolato
Department of Biomedical Sciences, National Research Center for Biomembranes, University of Padova, I 35121 Padova, Italy
Mol Biol Cell 9:1513-22. 1998..The present results are compatible with a major Ca2+-buffering role of CRT within the ER but exclude a direct, or indirect, role of this protein on the mechanism of ICRAC activation...
- New light on mitochondrial calciumP Pinton
Department of Biomedical Sciences, University of Padova, Italy
Biofactors 8:243-53. 1998..By using an imaging system endowed with high speed and sensitivity, which allows to obtain high-resolution 3D images, we could demonstrate that close contacts (< 80 nm) occur in vivo between mitochondria and the ER...
- The Ca2+ concentration of the endoplasmic reticulum is a key determinant of ceramide-induced apoptosis: significance for the molecular mechanism of Bcl-2 actionP Pinton
Department of Biomedical Sciences and CNR Center for the Study of Biomembranes, University of Padova, Via Colombo 3, I-35121 Padova, Italy
EMBO J 20:2690-701. 2001..It is therefore concluded that the Bcl-2-dependent reduction of [Ca2+]er is an important component of the anti-apoptotic program controlled by this oncogene...
- Cytosolic calcium oscillations in astrocytes may regulate exocytotic release of glutamateL Pasti
Department of Experimental Biomedical Sciences and Consiglio Nazionale delle Ricerche Center for the Study of Biomembranes, University of Padova, 35121 Padova, Italy
J Neurosci 21:477-84. 2001....
- Imaging signal transduction in living cells with GFP-based probesM Zaccolo
Dipartimento di Scienze Biomediche Sperimentali and Centro CNR per le Biomembrane, University of Padova, Italy
IUBMB Life 49:375-9. 2000..These genetically manipulated GFPs allow monitoring of the biochemistry of the cell in real time and thus offer the possibility to gain a more precise view of the functioning of live cells...
- Targeted recombinant aequorins: tools for monitoring [Ca2+] in the various compartments of a living cellM Brini
Department of Biochemistry, University of Padova, 35121 Padova, Italy
Microsc Res Tech 46:380-9. 1999....
- Alteration in calcium handling at the subcellular level in mdx myotubesV Robert
Department of Biomedical Sciences, CNR Center of Biomembranes, University of Padova, 35131 Padua, Italy
J Biol Chem 276:4647-51. 2001..Taking into account the key role played by mitochondria Ca(2+) handling in the control of cell death, our data suggest that mitochondria are potential targets of impaired Ca(2+) homeostasis in muscular dystrophy...
- Photodegradation of indo-1 and its effect on apparent Ca2+ concentrationsW J Scheenen
Department of Biomedical Sciences, University of Padova, Via Trieste 75, 35121 Padova, Italy
Chem Biol 3:765-74. 1996..Light-induced alteration in the behavior of the dye or cells would severely hamper the interpretation of experimental data. We examined this phenomenon in indo-1 loaded cells using confocal laser scanning microscopy...
- Role of capacitative calcium entry on glutamate-induced calcium influx in type-I rat cortical astrocytesP Pizzo
Department of Biomedical Sciences and the National Research Council Center for the Study of Biomembranes, University of Padua, Padua, Italy
J Neurochem 79:98-109. 2001..Despite the similar pharmacological properties of CCE in these cells, the pattern of TRP expression is very different. The involvement of CCE and TRPs in glutamate dependent activation of astrocytes is discussed...
- Ca2+ depletion from granules inhibits exocytosis. A study with insulin-secreting cellsW J Scheenen
Department of Biomedical Sciences, Consiglio Nazionale delle Ricerche, Center of Biomembranes, University of Padova, Via G Colombo 3, 35100 Padova Italy
J Biol Chem 273:19002-8. 1998..In contrast, releasing Ca2+ from the acidic pool markedly reduces secretion. It is suggested that a high Ca2+ concentration in the secretory compartment is needed to sustain optimal exocytosis...
- Calcium imaging of muscle cells treated with snake myotoxins reveals toxin synergism and presence of acceptorsM Cintra-Francischinelli
Dipartimento di Scienze Biomediche, Universita di Padova, Viale G Colombo, 3, 35121, Padova, Italy
Cell Mol Life Sci 66:1718-28. 2009..Therefore, these myotoxins may bind to acceptor(s) coupled to intracellular Ca(2+) mobilization in both myoblasts and myotubes. However, in myotubes only, the toxins alter plasma membrane permeability, leading to death...
- Heterogeneity of second messenger levels in living cellsM Zaccolo
Department of Biomedical Sciences, CNR Centre of Biomembranes, University of Padua, Italy
Novartis Found Symp 239:85-93; discussion 93-5, 150-9. 2001..We also demonstrate that by using such cAMP sensor, localized increase of this second messenger can be observed upon selective stimulation of plasma membrane receptors...
- Mitochondrial Ca2+ as a key regulator of cell life and deathM Giacomello
Department of Biomedical Sciences and CNR Institute of Neurosciences, University of Padua, Viale G Colombo 3, Padua, Italy
Cell Death Differ 14:1267-74. 2007..Finally, the potential involvement of Ca(2+) and mitochondria in the development of two diseases, Ullrich muscular dystrophy and familial Alzheimer's disease, is briefly discussed...
- Calreticulin is a candidate for a calsequestrin-like function in Ca2(+)-storage compartments (calciosomes) of liver and brainS Treves
Institute of General Pathology, University of Padova, Italy
Biochem J 271:473-80. 1990..Because of its properties and intraluminal location, calreticulin might play a critical role in Ca2+ storage and release in non-muscle cells, similar to that played by calsequestrin in the muscle sarcoplasmic reticulum...