Research Topics
Genomes and GenesSpecies | F PolticelliSummaryAffiliation: University Roma Tre Country: Italy Publications
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Detail Information
Publications
GA/GB fold switching may modulate fatty acid transfer from human serum albumin to bacteriaFabio Polticelli
Dipartimento di Biologia, Universita Roma Tre, Roma, Italy Istituto Nazionale di Fisica Nucleare, Sezione di Roma Tre, Roma, Italy
IUBMB Life 64:scope. 2012..2012 IUBMB. IUBMB Life, 64(11): 885-888, 2012...- GA/GB Fold switching may modulate fatty acid transfer from human serum albumin to bacteriaFabio Polticelli
Dipartimento di Biologia, Universita Roma Tre, Roma, Italy Istituto Nazionale di Fisica Nucleare, Sezione di Roma Tre, Roma, Italy
IUBMB Life 64:885-8. 2012..2012 IUBMB. IUBMB Life, 64(11): 885-888, 2012... 
Spermine oxidase (SMO) activity in breast tumor tissues and biochemical analysis of the anticancer spermine analogues BENSpm and CPENSpmManuela Cervelli
Dipartimento di Biologia, Universita Roma Tre, Rome, Italy
BMC Cancer 10:555. 2010..Biochemical analysis of Spm analogues BENSpm and CPENSpm, utilized in anticancer therapy, was also carried out to test their property in silico and in vitro on the recombinant SMO enzyme...- Massive non-natural proteins structure prediction using grid technologiesGiovanni Minervini
Department of Biology, University Roma Tre, Viale G, Marconi 446, Rome, I 00146, Italy
BMC Bioinformatics 10:S22. 2009..Thus, the structural study of NBPs can help to understand if natural protein sequences were selected for their peculiar properties or if they are just one of the possible stable and functional ensembles... - Human haptoglobin structure and function--a molecular modelling studyF Polticelli
Department of Biology and Interdepartmental Laboratory for Electron Microscopy, University Roma Tre, Italy
FEBS J 275:5648-56. 2008..The structural details of the models, which represent the first complete view of the molecular details of human haptoglobin variants, are discussed in relation to the known haptoglobin function(s)... 
Probing the cruzain S2 recognition subsite: a kinetic and binding energy calculation studyFabio Polticelli
Dipartimento di Biologia and Laboratorio Interdipartimentale per la Microscopia Elettronica, Universita Roma Tre, Viale Guglielmo Marconi 446, I 00146 Roma, Italy
Biochemistry 44:2781-9. 2005..These results appear of general significance for a deeper understanding of (macro)molecular recognition and for the rational design of novel inhibitors of parasitic cysteine proteases...- Modulation of the catalytic activity of cruzipain, the major cysteine proteinase from Trypanosoma cruzi, by temperature and pHL Salvati
Dipartimento di Biologia, , Roma, Italy
Eur J Biochem 268:3253-8. 2001..However, allosteric effect(s) cannot be excluded... - Serine proteinase inhibition by the active site titrant N alpha-(N, N-dimethylcarbamoyl)-alpha-azaornithine p-nitrophenyl ester. A comparative studyP Ascenzi
Department of Biology, University of Rome Tre, Italy
Eur J Biochem 267:1239-46. 2000..e. to P1-S1 recognition). These data represent the first detailed comparative investigation on the catalytic properties of serine proteinases towards an ornithine derivative (i. e. Dmc-azaOrn-ONp)... - May monomeric proteins be allosterically controlled?Paolo Ascenzi
IUBMB Life 54:223-4. 2002 - The Rhodococcus sp. cocaine esterase: a bacterial candidate for novel pharmacokinetic-based therapies for cocaine abusePaolo Ascenzi
Department of Biology, University Roma Tre, Viale G Marconi 446, I 00146 Roma, Italy
IUBMB Life 55:397-402. 2003..The discovery of the cocaine esterase from the strain MBI of the bacterium Rhodococcus sp. (cocE) could be a major breakthrough in this field; cocE hydrolyzes cocaine faster than any known cocaine esterase and catalytic antibody... - Characterization of a lysine-specific histone demethylase from Arabidopsis thalianaValentina Spedaletti
Department of Biology, University Roma Tre, 00146 Rome, Italy
Biochemistry 47:4936-47. 2008..This observation, together with AtLSD1 peculiar surface electrostatic potential distribution, suggests that the molecular partners of AtLSD1 are probably different from those of the human orthologue... - Heterologous expression and biochemical characterization of a polyamine oxidase from Arabidopsis involved in polyamine back conversionParaskevi Tavladoraki
Department of Biology, University Roma Tre, 00146 Rome, Italy
Plant Physiol 141:1519-32. 2006..To our knowledge, AtPAO1 is the first plant PAO reported to be involved in a polyamine back-conversion pathway... - Cytochromes: Reactivity of the "dark side" of the hemePaolo Ascenzi
Department of Biology, University Roma Tre, Viale Guglielmo Marconi 446, I 00146 Roma, Italy
Biophys Chem 152:21-7. 2010..Here, structural and functional aspects of NO binding to ferrous Axcyt c' and (CL-)hhcyt c are reviewed... - A barley polyamine oxidase isoform with distinct structural features and subcellular localizationM Cervelli
Dipartimento di Biologia, , Rome, Italy
Eur J Biochem 268:3816-30. 2001..We conclude that BPAO2 is a symplastic PAO in barley mesophyll cells. Production of BPAO2 mRNA and the corresponding protein is induced by light, and has a different pattern of accumulation in leaves and coleoptiles... - Inhibition of Saccharomyces cerevisiae phosphomannose isomerase by the NO-donor S-nitroso-acetyl-penicillamineL Salvati
Dipartimento di Biologia, , Viale Guglielmo Marconi 446, I-00146 Roma, Italy
J Enzyme Inhib 16:287-92. 2001..The analysis of the X-ray crystal structure of C. albicans PMI and of the molecular model of S. cerevisiae PMI provides a rationale for the low reactivity of Cys150 towards alkylating and nitrosylating agents... - Inhibition of cysteine protease activity by NO-donorsP Ascenzi
Dipartimento di Biologia, Universita Roma Tre, Viale G. Marconi 446, Italy
Curr Protein Pept Sci 2:137-53. 2001..Here, inhibition of representative cysteine proteases by NO(-donors) is reviewed... - Molecular basis for the binding of competitive inhibitors of maize polyamine oxidaseAlessandra Cona
Dipartimento di Biologia, , Viale Guglielmo Marconi 446, I-00146 Roma, Italy
Biochemistry 43:3426-35. 2004..Finally, docking simulations performed with the charged and uncharged forms of MPAO inhibitors indicate that the stereoelectronic properties of the MPAO active site are consistent with the binding of inhibitors in the protonated form... - Inhibition of polyamine and spermine oxidases by polyamine analoguesMarzia Bianchi
Dipartimento di Biologia, Universita Roma Tre, Italy
FEBS J 273:1115-23. 2006..Thus, the data reported here provide the basis for the development of novel and selective inhibitors able to discriminate between mammalian SMO and PAO activities... - Contryphan-Vn: a novel peptide from the venom of the Mediterranean snail Conus ventricosusG R Massilia
Department of Biology, University Roma Tre, Viale G. Marconi 446, Rome, 00146, Italy
Biochem Biophys Res Commun 288:908-13. 2001..Although the biological function(s) of contryphans is still unknown, these characteristics suggest distinct molecular target(s) and/or function(s) for Contryphan-Vn... - Two short protein domains are responsible for the nuclear localization of the mouse spermine oxidase mu isoformMarzia Bianchi
Dipartimento di Biologia, Universita Roma Tre, Roma, Italy
FEBS J 272:3052-9. 2005..A deletion mutant enzyme of this region was constructed to demonstrate its role in protein nuclear targeting by means of transient expression in the murine neuroblastoma cell line, N18TG2... - Heterologous expression and characterization of mouse spermine oxidaseManuela Cervelli
Dipartimento di Biologia, Universita Roma Tre, I 00146 Roma, Italy
J Biol Chem 278:5271-6. 2003..0. Molecular modeling of mSMO protein based on maize polyamine oxidase three-dimensional structure suggests that the general features of maize polyamine oxidase active site are conserved in mSMO... - Mitochondrial type I nitric oxide synthase physically interacts with cytochrome c oxidaseTiziana Persichini
Department of Biology LIME, University Roma Tre, Viale Guglielmo Marconi 446, 00146 Rome, Italy
Neurosci Lett 384:254-9. 2005..The molecular details of the interaction have been analyzed by means of molecular modeling and molecular dynamics simulations. This is the first evidence of a protein-protein interaction mediated by PDZ domains involving CcOX... - Cupryphans, metal-binding, redox-active, redesigned conopeptidesMarco Barba
Department of Biology, University Roma Tre, Rome, Italy
Protein Sci 18:559-68. 2009..We conclude that the Contryphan motif represents a natural robust scaffold which can be engineered to perform different functions, providing additional means for the design of catalytically active mini metalloproteins... - The R215W mutation in NBS1 impairs gamma-H2AX binding and affects DNA repair: molecular bases for the severe phenotype of 657del5/R215W Nijmegen breakage syndrome patientsAlessandra di Masi
Department of Biology, University Roma Tre, Viale Guglielmo Marconi 446, I 00146 Roma, Italy
Biochem Biophys Res Commun 369:835-40. 2008..Present data represent the first evidence for the role of NBS1 tandem BRCT domains in gamma-H2AX recognition, and could explain the severe phenotype observed in 657del5/R215W NBS patients... - Mouse spermine oxidase gene splice variants. Nuclear subcellular localization of a novel active isoformManuela Cervelli
Dipartimento di Biologia, Universita Roma Tre, Rome, Italy
Eur J Biochem 271:760-70. 2004..In addition, the relative transcription levels of the gene splicing variants were evaluated by RT-PCR analysis to verify a relationship with the SMO enzyme activity in various murine organs... - Lys300 plays a major role in the catalytic mechanism of maize polyamine oxidaseFabio Polticelli
Department of Biology, University Roma Tre, Viale G Marconi 446, 00146 Rome, Italy
Biochemistry 44:16108-20. 2005..Our studies indicate the importance of Lys300 and probable importance of HOH309 to the mechanism of flavin reduction in MPAO. Possible roles for Lys300 and water in the mechanism of flavin reduction are discussed... - The production of de novo folded proteins by a stepwise chain elongation: a model for prebiotic chemical evolution of macromolecular sequencesSalvatore Chessari
Department of Materials, Swiss Federal Institute of Technology, ETHZ,
Chem Biodivers 3:1202-10. 2006..The implication of this procedure as a general mechanism for the etiology of de novo macromolecular sequences and globular proteins in the origin of life is briefly discussed... - AtCYS1, a cystatin from Arabidopsis thaliana, suppresses hypersensitive cell deathBeatrice Belenghi
Dipartimento Scientifico e Tecnologico, , Verona, Italy
Eur J Biochem 270:2593-604. 2003.... - Conus ventricosus venom peptides profiling by HPLC-MS: a new insight in the intraspecific variationCristina Romeo
Consorzio Interuniversitario Istituto Nazionale Biostrutture e Biosistemi, Rome, Italy
J Sep Sci 31:488-98. 2008..Similarly, molecular modeling data suggest that -Conotoxin-Vn should represent a competitive antagonist of neuronal nicotinic acetylcholine receptors (nAChRs)... - Investigation of de novo totally random biosequences, Part II: On the folding frequency in a totally random library of de novo proteins obtained by phage displayCristiano Chiarabelli
Centro Studi e Ricerche E. Fermi, Via Panisperna 89A, IT-00184 Rome
Chem Biodivers 3:840-59. 2006..The consequences of this finding within a library of 'Never Born Proteins' are discussed in terms of molecular evolution... - ATP specifically drives refolding of non-native conformations of cytochrome cFederica Sinibaldi
Dipartimento di Medicina Sperimentale e Scienze Biochimiche, , Via Montpellier 1, 00133 Rome, Italy
Protein Sci 14:1049-58. 2005..The finding that ATP and dATP are unique among the nucleotides in being able to turn non-native states of cyt c back to native conformation is discussed in the light of cyt c involvement in cell apoptosis... - 1H NMR relaxometric characterization of bovine lactoferrinMauro Fasano
Dipartimento di Biologia Strutturale e Funzionale, Universita dell Insubria, Via Alberto da Giussano 12, I 21052 Busto Arsizio VA, Italy
J Inorg Biochem 98:1421-6. 2004..By increasing the pH from 6.5 to 12 two distinct titrations are observed, consistent with sequential removal of both water molecules...