Research Topics
Species | P PinzaniSummaryAffiliation: University of Florence Country: Italy Publications
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Publications
Circulating cell-free DNA in plasma of melanoma patients: qualitative and quantitative considerationsPamela Pinzani
Department of Clinical Physiopathology, University of Florence, Viale Pieraccini 6, 50139 Florence, Italy
Clin Chim Acta 412:2141-5. 2011..In conclusion, DNA integrity indexes can be considered suitable parameters for monitoring cfDNA fragmentation in melanoma patients...
Isolation by size of epithelial tumor cells in peripheral blood of patients with breast cancer: correlation with real-time reverse transcriptase-polymerase chain reaction results and feasibility of molecular analysis by laser microdissectionPamela Pinzani
Clinical Biochemistry Unit, Department of Clinical Physiopathology, University of Florence, 50100 Florence, Italy
Hum Pathol 37:711-8. 2006..Moreover, the laser microdissection technique allows for the recovery of nucleic acids for further molecular analysis and CTC characterization...- Healthy centenarian subjects: the effect of red wine consumption on liver function testsP Pinzani
Department of Critical Care Medicine and Surgery, Gerontology and Geriatrics Unit, University of Florence, 50139 Florence, Italy
J Endocrinol Invest 28:120-2. 2005..We conclude that red wine consumption in centenarians had no negative effect on circulating liver enzyme activities... - Red or white wine assumption and serum antioxidant capacityP Pinzani
Department of Clinical Physiopathology, Clinical Biochemistry Unit, University of Florence, Viale Pieraccini 6, I 50139 Florence, Italy
Arch Gerontol Geriatr 51:e72-4. 2010.... - Tyrosinase mRNA levels in the blood of uveal melanoma patients: correlation with the number of circulating tumor cells and tumor progressionPamela Pinzani
Department of Clinical Physiopathology, Centre of Experimental and Clinical Oncology, University of Florence, Florence, Italy
Melanoma Res 20:303-10. 2010..The mRNA tyrosinase levels can be taken as an indirect parameter correlated to the number of CTC isolated from blood by ISET... - Serum total antioxidant capacity, DHEAS and IGF-I in healthy centenarian subjectsP Pinzani
Gerontology and Geriatrics Unit, Dept. of Critical Care Medicine and Surgery, University of Florence, Florence, Italy
J Endocrinol Invest 25:83-4. 2002 - Allele specific Taqman-based real-time PCR assay to quantify circulating BRAFV600E mutated DNA in plasma of melanoma patientsPamela Pinzani
Department of Clinical Physiopathology, University of Florence, Viale Pieraccini 6, 50139 Florence, Italy
Clin Chim Acta 411:1319-24. 2010..As this event occurs early in melanoma progression, the quantification of BRAF-mutated alleles in plasma may represent a useful biomarker for noninvasive diagnosis and prediction of response to therapy... - BRAFV600E detection in melanoma is highly improved by COLD-PCRPamela Pinzani
Department of Clinical Physiopathology, University of Florence, Viale Pieraccini 6, 50139 Florence, Italy
Clin Chim Acta 412:901-5. 2011..Our aim was to investigate COLD-PCR (co-amplification at lower denaturation temperature-PCR) as a new approach for the pre-analytical enrichment of the BRAFV600E variant in formalin fixed paraffin embedded (FFPE) melanoma tissues... - Type-2 somatostatin receptor mRNA levels in breast and colon cancer determined by a quantitative RT-PCR assay based on dual label fluorogenic probe and the TaqMan technologyP Pinzani
Clinical Biochemistry, Department of Clinical Physiopathology, University of Florence, Viale Pieraccini 6, 50139, Florence, Italy
Regul Pept 99:79-86. 2001..For a correct evaluation of sst2 mRNA expression, it seems very important to measure the sst2 expression both in tumor and in the non-tumoral non-adjacent tumor specimens... - [Somatostatin receptors in non-endocrine tumours]C Casini Raggi
, , Florence, Italy
Minerva Endocrinol 26:149-58. 2001..In breast cancer, it is possible that sensitivity to estrogens may have a positive influence on the expression of sst2. This might justify clinical trials with ss in breast cancer... - Effect of diet and red wine consumption on serum total antioxidant capacity (TAC), dehydroepiandrosterone-sulphate (DHEAS) and insulin-like growth factor-1 (IGF-1) in Italian centenariansF M Antonini
Gerontology and Geriatrics Unit, Department of Critical Care Medicine and Surgery, University of Florence, Viale Pieraccini 6, I-50139 Firenze, Italy
Arch Gerontol Geriatr 41:151-7. 2005.... - SDH mutations in patients affected by paraganglioma syndromes: a personal experienceM Mannelli
Endocrinology Section, Department of Clinical Physiopathology, University of Florence, Viale Pieraccini 6 50139, Florence, and Internal Medicine 2, Pistoia Hospital, Italy
Ann N Y Acad Sci 1073:183-9. 2006..All the tumors associated with SDHD mutations were benign. Conversely, the only mutation we found in SDHB gene (IVS3+1G>A) was associated with a malignant PHEO... - Physical performance and creative activities of centenariansF M Antonini
Gerontology and Geriatrics Unit, Department of Critical Care Medicine and Surgery, University of Florence, Viale Pieraccini 6, I 50139 Florence, Italy
Arch Gerontol Geriatr 46:253-61. 2008.... - Prostate-specific antigen mRNA and protein levels in laser microdissected cells of human prostate measured by real-time reverse transcriptase-quantitative polymerase chain reaction and immuno-quantitative polymerase chain reactionPamela Pinzani
Department of Clinical Physiopathology, Clinical Biochemistry Unit, University of Florence, Italy
Hum Pathol 39:1474-82. 2008..The approach is expected to become a very powerful tool for expression studies in human cancers at both mRNA and protein levels... - Characterization of the functional and growth properties of long-term cell cultures established from a human somatostatinomaG Galli
Department of Internal Medicine, University of Florence Medical School, Viale Pieraccini 6, 50139 Florence, Italy
Endocr Relat Cancer 13:79-93. 2006..Based on these findings, the potential of SST analogues, IFN-gamma and AZT, alone or in combination, should be further explored in the medical treatment of somatostatinoma... - Real-time PCR detection of Biscogniauxia mediterranea in symptomless oak tissueN Luchi
Dipartimento di Biotecnologie Agrarie, Sezione di Patologia vegetale, , Piazzale delle Cascine, Florence, Italy
Lett Appl Microbiol 41:61-8. 2005..SIGNIFICANCE AND IMPACT OF THE STUDY: This diagnostic method is a precise tool to localize fungi in symptomless plant tissues and promises to advance our understanding of fungal infection during their latent phase... - Human gastric epithelium produces IL-4 and IL-4delta2 isoform only upon Helicobacter pylori infectionB Orsini
Department of Clinical Pathophysiology, Gastroenterology Unit, University of Florence, Italy
Int J Immunopathol Pharmacol 20:809-18. 2007..pylori-infected patients. Data suggest that gastric epithelial cells might regulate the balance between tolerance and immune response by the fine tuning of IL-4 and IL-4delta2 expression... - Laser-assisted microdissection for real-time PCR sample preparationP Pinzani
Department of Clinical Physiopathology, Clinical Biochemistry Unit, University of Florence, Italy
Mol Aspects Med 27:140-59. 2006..Trying to highlight the advantages and disadvantages of LMD, the main problems related to specimens collection and processing, section preparation and extraction of bio-molecules from microdissected tissue samples have been analysed... - Rapid detection of Oenococcus oeni in wine by real-time quantitative PCRP Pinzani
Dipartimento di Fisiopatologia Clinica, Unità di Biochimica Clinica, Universita degli Studi di Firenze, Viale Pieraccini, Florence, Italy
Lett Appl Microbiol 38:118-24. 2004..To develop a real-time polymerase chain reaction (PCR) method for rapid detection and quantification of Oenococcus oeni in wine samples for monitoring malolactic fermentation... - Measurement of somatostatin receptor subtype 2 mRNA in breast cancer and corresponding normal tissueC Orlando
Clinical Biochemistry Unit, Department of Clinical Physiopathology, University of Florence, Florence, Italy
Endocr Relat Cancer 11:323-32. 2004..Featuring a possible role of somatostatin analogs in combined endocrine therapies for breast cancer, our results seem to confirm that the sst2 status of the tumor should be previously investigated... - c-Kit expression in patients with uterine leiomyosarcomas: a potential alternative therapeutic treatmentMaria Rosaria Raspollini
Department of Human Pathology and Oncology, University of Florence, Florence, Italy
Clin Cancer Res 10:3500-3. 2004.... - Distribution of Tankyrase-1 mRNA expression in colon cancer and its prospective correlation with progression stageStefania Gelmini
Clinical Biochemistry Unit, Department of Clinical Physiopathology, University of Florence, Florence 50139, Italy
Oncol Rep 16:1261-6. 2006..Survival analysis indicated that lower Tankyrase-1 mRNA expression in colon cancers was significantly associated to reduced patient survival (p=0.019) and disease-free interval (p=0.035), confirmed also in a multi-variate analysis... - Application of a filtration- and isolation-by-size technique for the detection of circulating tumor cells in cutaneous melanomaVincenzo de Giorgi
Department of Critical Care Medicine and Surgery, Dermatology Unit, University of Florence, Florence, Italy
J Invest Dermatol 130:2440-7. 2010..001). CTC detection corroborated by suitable molecular characterization may assist in the identification and monitoring of more appropriate therapies in melanoma patients... - Circulating benign nevus cells detected by ISET technique: warning for melanoma molecular diagnosisVincenzo de Giorgi
Department of Dermatology, University of Florence, Via della Pergola 60, 50132 Florence, Italy
Arch Dermatol 146:1120-4. 2010..Isolation by size of epithelial tumor cells (ISET) allows the identification of circulating tumor cells by filtration according to size... - Influence of 17q gain and promoter polymorphisms on mRNA expression of somatostatin receptor type 2 in neuroblastomaLisa Simi
Clinical Biochemistry Units, Department of Clinical Physiopathology, University of Florence, Italy
Clin Chim Acta 384:149-54. 2007..Several genetic abnormalities are detected in neuroblastomas, frequently involving balanced and/or unbalanced gain on the long arm on chromosome 17, the same region containing sst2 gene... - Effects of gemcitabine in normal and transformed human lung cell cultures: cytotoxicity and increase in radiation sensitivityS Pacini
Department of Anatomy, Histology and Forensic Medicine, University of Florence, Policlinico di Careggi, Italy
Tumori 85:503-7. 1999..In this study we have investigated whether gemcitabine may play a role as radiosensitizer also in lung adenocarcinoma treatment... - A chemiluminescent method for the measurement of pregnanetriol-3 alpha-glucuronide in human diluted urineP Pinzani
Department of Clinical Physiopathology, University of Florence, Italy
J Biolumin Chemilumin 4:580-6. 1989..PTG levels well correlated with 17OHP plasma concentrations both under basal and stimulated conditions, in normal women as well as in the patient affected by CAH... - Quantitative real-time reverse transcription polymerase chain reaction: normalization to rRNA or single housekeeping genes is inappropriate for human tissue biopsiesCarmela Tricarico
Clinical Biochemistry Unit, Department of Clinical Physiopathology, University of Florence, Florence, Italy
Anal Biochem 309:293-300. 2002..Our results suggest that the use of internal standards comprising single housekeeping genes or rRNA is inappropriate for studies involving tissue biopsies... - Comparison of pre- and postsurgical concentrations of blood HER-2 mRNA and HER-2 extracellular domain reflects HER-2 status in early breast cancerBenedetta Salvadori
Clinical Biochemistry Unit, Department of Clinical Physiopathology, University of Florence, 50139 Florence, Italy
Clin Chem 51:254-6. 2005 - Circulating nucleic acids in cancer and pregnancyPamela Pinzani
Department of Clinical Physiopathology, University of Florence and Istituto Toscano Tumori, Viale Pieraccini 6, 50139 Florence, Italy
Methods 50:302-7. 2010..Recent reports on the importance of circulating nucleic acids in the intercellular exchange of genetic information between eukaryotic cells have been reviewed... - Clinical, biochemical and therapeutical aspects of amiodarone-induced hypothyroidism (AIH) in geriatric patients with cardiac arrhythmiasR G Gheri
Endocrinology Unit, Department of Clinical Physiopathology, University of Florence, Viale Pieraccini 6, Florence I-50139, Italy
Arch Gerontol Geriatr 38:27-36. 2004..In conclusion, in amiodarone treated elderly people, diagnosis of hypothyroidism is reliable only on the basis of high values of TSH; clinical features and fT3 serum levels never enable diagnosis... 
Phenotype variability of neural crest derived tumours in six Italian families segregating the same founder SDHD mutation Q109XL Simi
Section of Endocrinology, Department of Clinical Physiopathology, University of Florence Medical School, Florence, Italy
J Med Genet 42:e52. 2005..CONCLUSIONS: This study shows that a single founder SDHD mutation is present in an area of central Italy and that this mutation is associated with widely variable interfamilial and intrafamilial expressivity...- External quality assurance program for PCR amplification of genomic DNA: an Italian experienceClaudia Casini Raggi
Department of Clinical Physiopathology, University of Florence, Viale Pieraccini 6, 50139 Florence, Italy
Clin Chem 49:782-91. 2003..CONCLUSIONS: This survey demonstrates the importance of EQA trials based on methodologic proficiency testing directed to evaluation of analytical aspects common to the majority of PCR-based tests... - Expression of cyclooxygenase-2 in osteosarcoma of boneLaura Masi
Department of Internal Medicine, University of Florence, Florence, Italy
Appl Immunohistochem Mol Morphol 15:70-6. 2007..These results indicate that COX-2 is expressed at high levels in high grade osteosarcomas and support the use of COX-2 inhibitors to improve both the tumor response to chemotherapy and the outcome of osteosarcoma patients... - An Italian program of external quality control for quantitative assays based on real-time PCR with Taq-Man probesClaudia Casini Raggi
Clinical Biochemistry Unit, Department of Clinical Physiopathology, University of Florence, Italy
Clin Chem Lab Med 43:542-8. 2005.... - Uterine leiomyosarcomas express KIT protein but lack mutation(s) in exon 9 of c-KITMaria Rosaria Raspollini
Gynecol Oncol 98:334-5. 2005