P Pinzani

Summary

Affiliation: University of Florence
Country: Italy

Publications

  1. doi request reprint Circulating BRAFV600E in the Diagnosis and Follow-Up of Differentiated Papillary Thyroid Carcinoma
    C Pupilli
    Department of Biomedical, Experimental, and Clinical Sciences, Viale Pieraccini 6, 50139 Florence, Italy
    J Clin Endocrinol Metab 98:3359-65. 2013
  2. ncbi request reprint Healthy centenarian subjects: the effect of red wine consumption on liver function tests
    P Pinzani
    Department of Critical Care Medicine and Surgery, Gerontology and Geriatrics Unit, University of Florence, 50139 Florence, Italy
    J Endocrinol Invest 28:120-2. 2005
  3. ncbi request reprint Isolation by size of epithelial tumor cells in peripheral blood of patients with breast cancer: correlation with real-time reverse transcriptase-polymerase chain reaction results and feasibility of molecular analysis by laser microdissection
    Pamela Pinzani
    Clinical Biochemistry Unit, Department of Clinical Physiopathology, University of Florence, 50100 Florence, Italy
    Hum Pathol 37:711-8. 2006
  4. doi request reprint Red or white wine assumption and serum antioxidant capacity
    P Pinzani
    Department of Clinical Physiopathology, Clinical Biochemistry Unit, University of Florence, Viale Pieraccini 6, I 50139 Florence, Italy
    Arch Gerontol Geriatr 51:e72-4. 2010
  5. doi request reprint Tyrosinase mRNA levels in the blood of uveal melanoma patients: correlation with the number of circulating tumor cells and tumor progression
    Pamela Pinzani
    Department of Clinical Physiopathology, Centre of Experimental and Clinical Oncology, University of Florence, Florence, Italy
    Melanoma Res 20:303-10. 2010
  6. doi request reprint Allele specific Taqman-based real-time PCR assay to quantify circulating BRAFV600E mutated DNA in plasma of melanoma patients
    Pamela Pinzani
    Department of Clinical Physiopathology, University of Florence, Viale Pieraccini 6, 50139 Florence, Italy
    Clin Chim Acta 411:1319-24. 2010
  7. ncbi request reprint Serum total antioxidant capacity, DHEAS and IGF-I in healthy centenarian subjects
    P Pinzani
    Gerontology and Geriatrics Unit, Dept of Critical Care Medicine and Surgery, University of Florence, Florence, Italy
    J Endocrinol Invest 25:83-4. 2002
  8. doi request reprint Circulating cell-free DNA in plasma of melanoma patients: qualitative and quantitative considerations
    Pamela Pinzani
    Department of Clinical Physiopathology, University of Florence, Viale Pieraccini 6, 50139 Florence, Italy
    Clin Chim Acta 412:2141-5. 2011
  9. doi request reprint BRAFV600E detection in melanoma is highly improved by COLD-PCR
    Pamela Pinzani
    Department of Clinical Physiopathology, University of Florence, Viale Pieraccini 6, 50139 Florence, Italy
    Clin Chim Acta 412:901-5. 2011
  10. ncbi request reprint Type-2 somatostatin receptor mRNA levels in breast and colon cancer determined by a quantitative RT-PCR assay based on dual label fluorogenic probe and the TaqMan technology
    P Pinzani
    Clinical Biochemistry, Department of Clinical Physiopathology, University of Florence, Viale Pieraccini 6, 50139, Florence, Italy
    Regul Pept 99:79-86. 2001

Collaborators

Detail Information

Publications37

  1. doi request reprint Circulating BRAFV600E in the Diagnosis and Follow-Up of Differentiated Papillary Thyroid Carcinoma
    C Pupilli
    Department of Biomedical, Experimental, and Clinical Sciences, Viale Pieraccini 6, 50139 Florence, Italy
    J Clin Endocrinol Metab 98:3359-65. 2013
    ..Conclusions: The results of the present study provide encouraging data supporting the possibility to take advantage of circulating BRAF(V600E) in the management of PTC. ..
  2. ncbi request reprint Healthy centenarian subjects: the effect of red wine consumption on liver function tests
    P Pinzani
    Department of Critical Care Medicine and Surgery, Gerontology and Geriatrics Unit, University of Florence, 50139 Florence, Italy
    J Endocrinol Invest 28:120-2. 2005
    ..We conclude that red wine consumption in centenarians had no negative effect on circulating liver enzyme activities...
  3. ncbi request reprint Isolation by size of epithelial tumor cells in peripheral blood of patients with breast cancer: correlation with real-time reverse transcriptase-polymerase chain reaction results and feasibility of molecular analysis by laser microdissection
    Pamela Pinzani
    Clinical Biochemistry Unit, Department of Clinical Physiopathology, University of Florence, 50100 Florence, Italy
    Hum Pathol 37:711-8. 2006
    ..Moreover, the laser microdissection technique allows for the recovery of nucleic acids for further molecular analysis and CTC characterization...
  4. doi request reprint Red or white wine assumption and serum antioxidant capacity
    P Pinzani
    Department of Clinical Physiopathology, Clinical Biochemistry Unit, University of Florence, Viale Pieraccini 6, I 50139 Florence, Italy
    Arch Gerontol Geriatr 51:e72-4. 2010
    ....
  5. doi request reprint Tyrosinase mRNA levels in the blood of uveal melanoma patients: correlation with the number of circulating tumor cells and tumor progression
    Pamela Pinzani
    Department of Clinical Physiopathology, Centre of Experimental and Clinical Oncology, University of Florence, Florence, Italy
    Melanoma Res 20:303-10. 2010
    ..The mRNA tyrosinase levels can be taken as an indirect parameter correlated to the number of CTC isolated from blood by ISET...
  6. doi request reprint Allele specific Taqman-based real-time PCR assay to quantify circulating BRAFV600E mutated DNA in plasma of melanoma patients
    Pamela Pinzani
    Department of Clinical Physiopathology, University of Florence, Viale Pieraccini 6, 50139 Florence, Italy
    Clin Chim Acta 411:1319-24. 2010
    ..As this event occurs early in melanoma progression, the quantification of BRAF-mutated alleles in plasma may represent a useful biomarker for noninvasive diagnosis and prediction of response to therapy...
  7. ncbi request reprint Serum total antioxidant capacity, DHEAS and IGF-I in healthy centenarian subjects
    P Pinzani
    Gerontology and Geriatrics Unit, Dept of Critical Care Medicine and Surgery, University of Florence, Florence, Italy
    J Endocrinol Invest 25:83-4. 2002
  8. doi request reprint Circulating cell-free DNA in plasma of melanoma patients: qualitative and quantitative considerations
    Pamela Pinzani
    Department of Clinical Physiopathology, University of Florence, Viale Pieraccini 6, 50139 Florence, Italy
    Clin Chim Acta 412:2141-5. 2011
    ..In conclusion, DNA integrity indexes can be considered suitable parameters for monitoring cfDNA fragmentation in melanoma patients...
  9. doi request reprint BRAFV600E detection in melanoma is highly improved by COLD-PCR
    Pamela Pinzani
    Department of Clinical Physiopathology, University of Florence, Viale Pieraccini 6, 50139 Florence, Italy
    Clin Chim Acta 412:901-5. 2011
    ..Our aim was to investigate COLD-PCR (co-amplification at lower denaturation temperature-PCR) as a new approach for the pre-analytical enrichment of the BRAFV600E variant in formalin fixed paraffin embedded (FFPE) melanoma tissues...
  10. ncbi request reprint Type-2 somatostatin receptor mRNA levels in breast and colon cancer determined by a quantitative RT-PCR assay based on dual label fluorogenic probe and the TaqMan technology
    P Pinzani
    Clinical Biochemistry, Department of Clinical Physiopathology, University of Florence, Viale Pieraccini 6, 50139, Florence, Italy
    Regul Pept 99:79-86. 2001
    ..For a correct evaluation of sst2 mRNA expression, it seems very important to measure the sst2 expression both in tumor and in the non-tumoral non-adjacent tumor specimens...
  11. ncbi request reprint Effect of diet and red wine consumption on serum total antioxidant capacity (TAC), dehydroepiandrosterone-sulphate (DHEAS) and insulin-like growth factor-1 (IGF-1) in Italian centenarians
    F M Antonini
    Gerontology and Geriatrics Unit, Department of Critical Care Medicine and Surgery, University of Florence, Viale Pieraccini 6, I 50139 Firenze, Italy
    Arch Gerontol Geriatr 41:151-7. 2005
    ....
  12. ncbi request reprint [Somatostatin receptors in non-endocrine tumours]
    C Casini Raggi
    , , Florence, Italy
    Minerva Endocrinol 26:149-58. 2001
    ..In breast cancer, it is possible that sensitivity to estrogens may have a positive influence on the expression of sst2. This might justify clinical trials with ss in breast cancer...
  13. ncbi request reprint SDH mutations in patients affected by paraganglioma syndromes: a personal experience
    M Mannelli
    Endocrinology Section, Department of Clinical Physiopathology, University of Florence, Viale Pieraccini 6 50139, Florence, and Internal Medicine 2, Pistoia Hospital, Italy
    Ann N Y Acad Sci 1073:183-9. 2006
    ..All the tumors associated with SDHD mutations were benign. Conversely, the only mutation we found in SDHB gene (IVS3+1G>A) was associated with a malignant PHEO...
  14. ncbi request reprint Physical performance and creative activities of centenarians
    F M Antonini
    Gerontology and Geriatrics Unit, Department of Critical Care Medicine and Surgery, University of Florence, Viale Pieraccini 6, I 50139 Florence, Italy
    Arch Gerontol Geriatr 46:253-61. 2008
    ....
  15. doi request reprint Prostate-specific antigen mRNA and protein levels in laser microdissected cells of human prostate measured by real-time reverse transcriptase-quantitative polymerase chain reaction and immuno-quantitative polymerase chain reaction
    Pamela Pinzani
    Department of Clinical Physiopathology, Clinical Biochemistry Unit, University of Florence, Italy
    Hum Pathol 39:1474-82. 2008
    ..The approach is expected to become a very powerful tool for expression studies in human cancers at both mRNA and protein levels...
  16. ncbi request reprint Measurement of somatostatin receptor subtype 2 mRNA in breast cancer and corresponding normal tissue
    C Orlando
    Clinical Biochemistry Unit, Department of Clinical Physiopathology, University of Florence, Florence, Italy
    Endocr Relat Cancer 11:323-32. 2004
    ..Featuring a possible role of somatostatin analogs in combined endocrine therapies for breast cancer, our results seem to confirm that the sst2 status of the tumor should be previously investigated...
  17. ncbi request reprint Real-time PCR detection of Biscogniauxia mediterranea in symptomless oak tissue
    N Luchi
    Dipartimento di Biotecnologie Agrarie, Sezione di Patologia vegetale, Universita degli Studi di Firenze, Piazzale delle Cascine, Florence, Italy
    Lett Appl Microbiol 41:61-8. 2005
    ..Real-time PCR, based on TaqMan chemistry, was used to detect Biscogniauxia mediterranea, a fungal pathogen that after a long endophytic phase may cause charcoal disease in oak trees...
  18. ncbi request reprint Laser-assisted microdissection for real-time PCR sample preparation
    P Pinzani
    Department of Clinical Physiopathology, Clinical Biochemistry Unit, University of Florence, Italy
    Mol Aspects Med 27:140-59. 2006
    ..Trying to highlight the advantages and disadvantages of LMD, the main problems related to specimens collection and processing, section preparation and extraction of bio-molecules from microdissected tissue samples have been analysed...
  19. ncbi request reprint Human gastric epithelium produces IL-4 and IL-4delta2 isoform only upon Helicobacter pylori infection
    B Orsini
    Department of Clinical Pathophysiology, Gastroenterology Unit, University of Florence, Italy
    Int J Immunopathol Pharmacol 20:809-18. 2007
    ..pylori-infected patients. Data suggest that gastric epithelial cells might regulate the balance between tolerance and immune response by the fine tuning of IL-4 and IL-4delta2 expression...
  20. ncbi request reprint Rapid detection of Oenococcus oeni in wine by real-time quantitative PCR
    P Pinzani
    Dipartimento di Fisiopatologia Clinica, Unità di Biochimica Clinica, Universita degli Studi di Firenze, Viale Pieraccini, Florence, Italy
    Lett Appl Microbiol 38:118-24. 2004
    ..To develop a real-time polymerase chain reaction (PCR) method for rapid detection and quantification of Oenococcus oeni in wine samples for monitoring malolactic fermentation...
  21. ncbi request reprint Influence of 17q gain and promoter polymorphisms on mRNA expression of somatostatin receptor type 2 in neuroblastoma
    Lisa Simi
    Clinical Biochemistry Units, Department of Clinical Physiopathology, University of Florence, Italy
    Clin Chim Acta 384:149-54. 2007
    ..Several genetic abnormalities are detected in neuroblastomas, frequently involving balanced and/or unbalanced gain on the long arm on chromosome 17, the same region containing sst2 gene...
  22. ncbi request reprint Distribution of Tankyrase-1 mRNA expression in colon cancer and its prospective correlation with progression stage
    Stefania Gelmini
    Clinical Biochemistry Unit, Department of Clinical Physiopathology, University of Florence, Florence 50139, Italy
    Oncol Rep 16:1261-6. 2006
    ..Survival analysis indicated that lower Tankyrase-1 mRNA expression in colon cancers was significantly associated to reduced patient survival (p=0.019) and disease-free interval (p=0.035), confirmed also in a multi-variate analysis...
  23. ncbi request reprint c-Kit expression in patients with uterine leiomyosarcomas: a potential alternative therapeutic treatment
    Maria Rosaria Raspollini
    Department of Human Pathology and Oncology, University of Florence, Florence, Italy
    Clin Cancer Res 10:3500-3. 2004
    ....
  24. doi request reprint Application of a filtration- and isolation-by-size technique for the detection of circulating tumor cells in cutaneous melanoma
    Vincenzo de Giorgi
    Department of Critical Care Medicine and Surgery, Dermatology Unit, University of Florence, Florence, Italy
    J Invest Dermatol 130:2440-7. 2010
    ..001). CTC detection corroborated by suitable molecular characterization may assist in the identification and monitoring of more appropriate therapies in melanoma patients...
  25. doi request reprint Circulating benign nevus cells detected by ISET technique: warning for melanoma molecular diagnosis
    Vincenzo de Giorgi
    Department of Dermatology, University of Florence, Via della Pergola 60, 50132 Florence, Italy
    Arch Dermatol 146:1120-4. 2010
    ..Isolation by size of epithelial tumor cells (ISET) allows the identification of circulating tumor cells by filtration according to size...
  26. ncbi request reprint Characterization of the functional and growth properties of long-term cell cultures established from a human somatostatinoma
    G Galli
    Department of Internal Medicine, University of Florence Medical School, Viale Pieraccini 6, 50139 Florence, Italy
    Endocr Relat Cancer 13:79-93. 2006
    ..Based on these findings, the potential of SST analogues, IFN-gamma and AZT, alone or in combination, should be further explored in the medical treatment of somatostatinoma...
  27. pmc Phenotype variability of neural crest derived tumours in six Italian families segregating the same founder SDHD mutation Q109X
    L Simi
    Section of Endocrinology, Department of Clinical Physiopathology, University of Florence Medical School, Florence, Italy
    J Med Genet 42:e52. 2005
    ..Mutations in genes coding for the mitochondrial complex II succinate dehydrogenase (SDH) subunits cause familial neural crest derived (NCD) tumours...
  28. ncbi request reprint Effects of gemcitabine in normal and transformed human lung cell cultures: cytotoxicity and increase in radiation sensitivity
    S Pacini
    Department of Anatomy, Histology and Forensic Medicine, University of Florence, Policlinico di Careggi, Italy
    Tumori 85:503-7. 1999
    ..In this study we have investigated whether gemcitabine may play a role as radiosensitizer also in lung adenocarcinoma treatment...
  29. ncbi request reprint A chemiluminescent method for the measurement of pregnanetriol-3 alpha-glucuronide in human diluted urine
    P Pinzani
    Department of Clinical Physiopathology, University of Florence, Italy
    J Biolumin Chemilumin 4:580-6. 1989
    ..PTG levels well correlated with 17OHP plasma concentrations both under basal and stimulated conditions, in normal women as well as in the patient affected by CAH...
  30. ncbi request reprint Quantitative real-time reverse transcription polymerase chain reaction: normalization to rRNA or single housekeeping genes is inappropriate for human tissue biopsies
    Carmela Tricarico
    Clinical Biochemistry Unit, Department of Clinical Physiopathology, University of Florence, Florence, Italy
    Anal Biochem 309:293-300. 2002
    ..Our results suggest that the use of internal standards comprising single housekeeping genes or rRNA is inappropriate for studies involving tissue biopsies...
  31. ncbi request reprint Comparison of pre- and postsurgical concentrations of blood HER-2 mRNA and HER-2 extracellular domain reflects HER-2 status in early breast cancer
    Benedetta Salvadori
    Clinical Biochemistry Unit, Department of Clinical Physiopathology, University of Florence, 50139 Florence, Italy
    Clin Chem 51:254-6. 2005
  32. doi request reprint Circulating nucleic acids in cancer and pregnancy
    Pamela Pinzani
    Department of Clinical Physiopathology, University of Florence and Istituto Toscano Tumori, Viale Pieraccini 6, 50139 Florence, Italy
    Methods 50:302-7. 2010
    ..Recent reports on the importance of circulating nucleic acids in the intercellular exchange of genetic information between eukaryotic cells have been reviewed...
  33. ncbi request reprint Clinical, biochemical and therapeutical aspects of amiodarone-induced hypothyroidism (AIH) in geriatric patients with cardiac arrhythmias
    R G Gheri
    Endocrinology Unit, Department of Clinical Physiopathology, University of Florence, Viale Pieraccini 6, Florence I 50139, Italy
    Arch Gerontol Geriatr 38:27-36. 2004
    ..In conclusion, in amiodarone treated elderly people, diagnosis of hypothyroidism is reliable only on the basis of high values of TSH; clinical features and fT3 serum levels never enable diagnosis...
  34. ncbi request reprint External quality assurance program for PCR amplification of genomic DNA: an Italian experience
    Claudia Casini Raggi
    Department of Clinical Physiopathology, University of Florence, Viale Pieraccini 6, 50139 Florence, Italy
    Clin Chem 49:782-91. 2003
    ..Development of EQA trials based on methodologic proficiency testing and directed to the evaluation of analytical aspects common to the majority of PCR-based tests may be valuable...
  35. ncbi request reprint An Italian program of external quality control for quantitative assays based on real-time PCR with Taq-Man probes
    Claudia Casini Raggi
    Clinical Biochemistry Unit, Department of Clinical Physiopathology, University of Florence, Italy
    Clin Chem Lab Med 43:542-8. 2005
    ....
  36. ncbi request reprint Expression of cyclooxygenase-2 in osteosarcoma of bone
    Laura Masi
    Department of Internal Medicine, University of Florence, Florence, Italy
    Appl Immunohistochem Mol Morphol 15:70-6. 2007
    ..These results indicate that COX-2 is expressed at high levels in high grade osteosarcomas and support the use of COX-2 inhibitors to improve both the tumor response to chemotherapy and the outcome of osteosarcoma patients...
  37. ncbi request reprint Uterine leiomyosarcomas express KIT protein but lack mutation(s) in exon 9 of c-KIT
    Maria Rosaria Raspollini
    Gynecol Oncol 98:334-5. 2005