C Montecucco

Summary

Affiliation: University of Padova
Country: Italy

Publications

  1. ncbi request reprint How do presynaptic PLA2 neurotoxins block nerve terminals?
    C Montecucco
    Centro CNR Biomembrane and Dipartimento di Scienze Biomediche, Universita di Padova, Via G Colombo 3, 35121 Padova, Italy
    Trends Biochem Sci 25:266-70. 2000
  2. ncbi request reprint Molecular and cellular mechanisms of action of the vacuolating cytotoxin (VacA) and neutrophil-activating protein (HP-NAP) virulence factors of Helicobacter pylori
    Cesare Montecucco
    Dipartimento di Scienze Biomediche, Universita di Padova, Via G Colombo 3, 35121 Padua, Italy
    Microbes Infect 5:715-21. 2003
  3. ncbi request reprint Living dangerously: how Helicobacter pylori survives in the human stomach
    C Montecucco
    Centro CNR Biomembrane e Dipartimento di Scienze Biomediche, Universita di Padova, Via G Colombo 3, 35121 Padova, Italy
    Nat Rev Mol Cell Biol 2:457-66. 2001
  4. ncbi request reprint Molecular and cellular activities of Helicobacter pylori pathogenic factors
    C Montecucco
    Centro CNR Biomembrane and Dipartimento di Scienze Biomediche, Universita di Padova, Italy
    FEBS Lett 452:16-21. 1999
  5. ncbi request reprint Protein toxins and membrane transport
    C Montecucco
    Centro CNR Biomembrane, Padova, Italy
    Curr Opin Cell Biol 10:530-6. 1998
  6. pmc Susceptibility of mitogen-activated protein kinase kinase family members to proteolysis by anthrax lethal factor
    G Vitale
    Centro CNR Biomembrane and Dipartimento di Scienze Biomediche, Universita di Padova, Via Trieste 75, 35121 Padova, Italy
    Biochem J 352:739-45. 2000
  7. pmc Selective increase of the permeability of polarized epithelial cell monolayers by Helicobacter pylori vacuolating toxin
    E Papini
    Centro CNR Biomembrane and Dipartimento di Scienze, Biomediche, Universita di Padova, 35121 Padova, Italy
    J Clin Invest 102:813-20. 1998
  8. pmc Tetanus toxin is a zinc protein and its inhibition of neurotransmitter release and protease activity depend on zinc
    G Schiavo
    Centro CNR Biomembrane, Universita di Padova, Italy
    EMBO J 11:3577-83. 1992
  9. ncbi request reprint Site-directed mutagenesis identifies active-site residues of the light chain of botulinum neurotoxin type A
    M Rigoni
    Dipartimento di Scienze Biomediche Sperimentali, , Viale G. Colombo, 35121 Padua, Italy
    Biochem Biophys Res Commun 288:1231-7. 2001
  10. ncbi request reprint The Helicobacter pylori neutrophil-activating protein is an iron-binding protein with dodecameric structure
    F Tonello
    Centro CNR Biomembrane and Dipartimento di Scienze Biomediche, Universita di Padova, I 35121, Italy
    Mol Microbiol 34:238-46. 1999

Detail Information

Publications101 found, 100 shown here

  1. ncbi request reprint How do presynaptic PLA2 neurotoxins block nerve terminals?
    C Montecucco
    Centro CNR Biomembrane and Dipartimento di Scienze Biomediche, Universita di Padova, Via G Colombo 3, 35121 Padova, Italy
    Trends Biochem Sci 25:266-70. 2000
    ....
  2. ncbi request reprint Molecular and cellular mechanisms of action of the vacuolating cytotoxin (VacA) and neutrophil-activating protein (HP-NAP) virulence factors of Helicobacter pylori
    Cesare Montecucco
    Dipartimento di Scienze Biomediche, Universita di Padova, Via G Colombo 3, 35121 Padua, Italy
    Microbes Infect 5:715-21. 2003
    ..Their activities are discussed in terms of tissue alterations, which promote the release of nutrients necessary for the growth and survival of the bacterium in its nutrient-poor ecological niche...
  3. ncbi request reprint Living dangerously: how Helicobacter pylori survives in the human stomach
    C Montecucco
    Centro CNR Biomembrane e Dipartimento di Scienze Biomediche, Universita di Padova, Via G Colombo 3, 35121 Padova, Italy
    Nat Rev Mol Cell Biol 2:457-66. 2001
    ..To succeed in these long-term associations, H. pylori has developed a unique set of virulence factors, which allow survival in a unique and hostile ecological niche--the human stomach...
  4. ncbi request reprint Molecular and cellular activities of Helicobacter pylori pathogenic factors
    C Montecucco
    Centro CNR Biomembrane and Dipartimento di Scienze Biomediche, Universita di Padova, Italy
    FEBS Lett 452:16-21. 1999
    ..The activities of these factors are discussed in terms of the need of the bacterium of increasing the supply of nutrients from the stomach lumen and from the mucosa...
  5. ncbi request reprint Protein toxins and membrane transport
    C Montecucco
    Centro CNR Biomembrane, Padova, Italy
    Curr Opin Cell Biol 10:530-6. 1998
    ..Their use is being extended to the study of the physiology of these processes in different cells and tissues, as well as to the intracellular pathways of membrane transport...
  6. pmc Susceptibility of mitogen-activated protein kinase kinase family members to proteolysis by anthrax lethal factor
    G Vitale
    Centro CNR Biomembrane and Dipartimento di Scienze Biomediche, Universita di Padova, Via Trieste 75, 35121 Padova, Italy
    Biochem J 352:739-45. 2000
    ..The implications of these findings for the biochemical activity and functional specificity of LF are discussed...
  7. pmc Selective increase of the permeability of polarized epithelial cell monolayers by Helicobacter pylori vacuolating toxin
    E Papini
    Centro CNR Biomembrane and Dipartimento di Scienze, Biomediche, Universita di Padova, 35121 Padova, Italy
    J Clin Invest 102:813-20. 1998
    ..It is proposed that induction by VacA of a selective permeabilization of the epithelial paracellular route to low molecular weight molecules and ions may serve to supply nutrients, which favor H. pylori growth in vivo...
  8. pmc Tetanus toxin is a zinc protein and its inhibition of neurotransmitter release and protease activity depend on zinc
    G Schiavo
    Centro CNR Biomembrane, Universita di Padova, Italy
    EMBO J 11:3577-83. 1992
    ..The present findings indicate that tetanus toxin, and possibly also the botulinum neurotoxins, are metalloproteases and that they block neurotransmitter release via this protease activity...
  9. ncbi request reprint Site-directed mutagenesis identifies active-site residues of the light chain of botulinum neurotoxin type A
    M Rigoni
    Dipartimento di Scienze Biomediche Sperimentali, , Viale G. Colombo, 35121 Padua, Italy
    Biochem Biophys Res Commun 288:1231-7. 2001
    ..The Phe-266 and Tyr-366 mutants have reduced proteolytic activity indicating a direct participation in the proteolytic reaction, and their possible role in catalysis is discussed...
  10. ncbi request reprint The Helicobacter pylori neutrophil-activating protein is an iron-binding protein with dodecameric structure
    F Tonello
    Centro CNR Biomembrane and Dipartimento di Scienze Biomediche, Universita di Padova, I 35121, Italy
    Mol Microbiol 34:238-46. 1999
    ..We therefore conclude that HP-NAP is an unusual, small, ferritin that folds into a four-helix bundle that oligomerizes into dodecamers with a central hole capable of binding up to 500 iron atoms per oligomer...
  11. ncbi request reprint Cell vacuolization induced by Helicobacter pylori VacA cytotoxin does not depend on late endosomal SNAREs
    M de Bernard
    Centro CNR Biomembrane and Dipartimento di Scienze Biomediche Sperimentali, Universita di Padova, Via G Colombo 3, 35121, Padova, Italy
    Cell Microbiol 4:11-8. 2002
    ..The process of vacuolization leads to disappearance of the large array of internal membranes of late endosomes. We suggest that most of the vacuole-limiting membrane derives from internal membranes...
  12. ncbi request reprint Active-site mutagenesis of tetanus neurotoxin implicates TYR-375 and GLU-271 in metalloproteolytic activity
    O Rossetto
    Centro CNR Biomembrane and Dipartimento di Scienze Biomediche, Universita di Padova, Via G Colombo 3, 35121, Padova, Italy
    Toxicon 39:1151-9. 2001
    ..Interestingly, the two Ala mutants are completely devoid of enzymatic activity. These results demonstrate that both Glu-271 and Tyr-375 are essential for the proteolytic activity of TeNT...
  13. ncbi request reprint Different mechanism of blockade of neuroexocytosis by presynaptic neurotoxins
    O Rossetto
    Department of Biomedical Sciences, University of Padova, Viale G Colombo 3, 35121 Padova, Italy
    Toxicol Lett 149:91-101. 2004
    ....
  14. ncbi request reprint Bacterial protein toxins and cell vesicle trafficking
    C Montecucco
    Centro CNR Biomembrane, Universita di Padova, Italy
    Experientia 52:1026-32. 1996
    ..Vacuoles originate from this compartment in a rab7-dependent process and swell because they are acidic and accumulate membrane-permeant amines...
  15. ncbi request reprint Vacuoles induced by Helicobacter pylori toxin contain both late endosomal and lysosomal markers
    M Molinari
    Centro CNR Biomembrane and Dipartimento di Scienze Biomediche, Universita di Padova, 35100 Padova, Italy
    J Biol Chem 272:25339-44. 1997
    ....
  16. pmc A Lys49-PLA2 myotoxin of Bothrops asper triggers a rapid death of macrophages that involves autocrine purinergic receptor signaling
    F Tonello
    Istituto di Neuroscienze, CNR Sezione di Padova, Padova, Italy
    Cell Death Dis 3:e343. 2012
    ..Based on present findings, a mechanism of cell death is proposed that can be extended to other cytolytic proteins and peptides...
  17. pmc The VacA toxin of Helicobacter pylori identifies a new intermediate filament-interacting protein
    M de Bernard
    Centro CNR Biomembrane and Dipartimento di Scienze Biomediche, Universita di Padova, Via G Colombo 3, 35121 Padova, Italy
    EMBO J 19:48-56. 2000
    ..The possible involvement of this novel protein in interactions between intermediate filaments and late endosomal compartments is discussed...
  18. ncbi request reprint Anthrax lethal factor cleaves the N-terminus of MAPKKs and induces tyrosine/threonine phosphorylation of MAPKs in cultured macrophages
    G Vitale
    Dipartimento di Scienze Biomediche, Universita di Padova, Italy
    Biochem Biophys Res Commun 248:706-11. 1998
    ..The possible implications of these findings for the cytolysis of macrophage cells induced by LF are discussed. These results open the way to the design and screening of specific inhibitors of LF...
  19. doi request reprint The anthrax lethal factor and its MAPK kinase-specific metalloprotease activity
    Fiorella Tonello
    Dipartimento di Scienze Biomediche Sperimentali, Istituto CNR di Neuroscienze, Universita di Padova, Viale G Colombo 3, 35131 Padova, Italy
    Mol Aspects Med 30:431-8. 2009
    ..A significant similarity between the metalloprotease domain of the lethal factor and of that of the clostridial neurotoxins has been noted and is discussed...
  20. pmc The neutrophil-activating protein (HP-NAP) of Helicobacter pylori is a protective antigen and a major virulence factor
    B Satin
    Centro Consiglio Nazionale Ricerche Biomembrane and Dipartimento di Scienze Biomediche, Universita di Padova, 35121 Padova, Italy
    J Exp Med 191:1467-76. 2000
    ..On the basis of these results, we conclude that HP-NAP is a virulence factor important for the H. pylori pathogenic effects at the site of infection and a candidate antigen for vaccine development...
  21. ncbi request reprint Virulence factors of Helicobacter pylori
    W G Dundon
    Centro CNR Biomembrane and Dipartimento di Scienze Biomediche, , Italy
    Int J Med Microbiol 290:647-58. 2001
    ..Recent advances in our understanding of the involvement of VacA, HP-NAP and the CagA/Type IV secretion system in the H. pylori-associated disease process are discussed in this review...
  22. ncbi request reprint Botulinum neurotoxin types A and E require the SNARE motif in SNAP-25 for proteolysis
    P Washbourne
    Centro C N R Biomembrane and Dipartimento di Scienze Biomediche, Universita di Padova, Padua, Italy
    FEBS Lett 418:1-5. 1997
    ....
  23. ncbi request reprint The multiple cellular activities of the VacA cytotoxin of Helicobacter pylori
    Marina de Bernard
    Dipartimento di Scienze Biomediche, Universita di Padova, Istituto Veneto di Medicina Molecolare, Padova, Italy
    Int J Med Microbiol 293:589-97. 2004
    ..This toxin also shows some pro-inflammatory and immunosuppressive activities which may be functional to the establishment of a chronic type of inflammation...
  24. ncbi request reprint Botulinum neurotoxins are metalloproteases specific for SNARE proteins involved in neuroexocytosis
    Ornella Rossetto
    Centro CNR Biomembrane, Dipartimento di Scienze Biomediche, Universita di Padova, Italia
    Curr Probl Dermatol 30:117-25. 2002
  25. pmc Potent inhibitors of anthrax lethal factor from green tea
    Isabella Dell'aica
    Dipartimento di Scienze Biomediche, Universita di Padova, Viale G Colombo 3, 35121 Padua, Italy
    EMBO Rep 5:418-22. 2004
    ....
  26. ncbi request reprint Screening inhibitors of anthrax lethal factor
    Fiorella Tonello
    Istituto CNR Neuroscienze, Dipartimento di Scienze Biomediche, Universita di Padova, Via G Colombo 3, 35121 Padova, Italy
    Nature 418:386. 2002
    ..Our assay should help to answer the urgent call for new and specific therapies to combat this pathogen after its recent emergence as a terrorist bioweapon...
  27. ncbi request reprint The neutrophil-activating protein (HP-NAP) of Helicobacter pylori is a potent stimulant of mast cells
    Pasqualina Montemurro
    Centro CNR Biomembrane and Dipartimento di Scienze Biomediche, Universita di Padova, Padova, Italy
    Eur J Immunol 32:671-6. 2002
    ..These findings characterize HP-NAP as an inflammatory factor of H.pylori that is effective from the beginning of the inflammatory cascade...
  28. ncbi request reprint Neutrophil-activating protein (HP-NAP) versus ferritin (Pfr): comparison of synthesis in Helicobacter pylori
    W G Dundon
    Centro CNR Biomembrane e Dipartimento di Scienze Biomediche, Universita di Padova, Italy
    FEMS Microbiol Lett 199:143-9. 2001
    ..Recombinant Pfr, as well as conferring resistance to iron and copper as previously reported, also conferred resistance to zinc overload when produced in E. coli...
  29. doi request reprint Cellular pathology induced by snake venom phospholipase A2 myotoxins and neurotoxins: common aspects of their mechanisms of action
    C Montecucco
    Instituto Clodomiro Picado, Facultad de Microbiologia, Universidad de Costa Rica, San Jose 2060, Costa Rica
    Cell Mol Life Sci 65:2897-912. 2008
    ..The different systemic pathophysiological consequences caused by these toxins are not due to different mechanisms of cell toxicity, but to the intrinsic anatomical and physiological properties of the targeted tissues and cells...
  30. ncbi request reprint [Italian Society of Rheumatology (SIR) recommendations for performing arthrocentesis]
    L Punzi
    Cattedra ed UOC Reumatologia, Universita di Padova, Italia
    Reumatismo 59:227-34. 2007
    ..Several of these recommendations were based on experts' opinion rather than on published evidence which is scanty...
  31. doi request reprint Calcium overload in nerve terminals of cultured neurons intoxicated by alpha-latrotoxin and snake PLA2 neurotoxins
    Erik Tedesco
    Dipartimento di Scienze Biomediche Sperimentali, Università di Padova and Istituto di Neuroscienze del Consiglio Nazionale delle Ricerche, Padova, Italy
    Toxicon 54:138-44. 2009
    ..This indicates that Ca(2+) overloading plays a major role in the degeneration of nerve terminals induced by the snake presynaptic neurotoxins...
  32. ncbi request reprint Presynaptic enzymatic neurotoxins
    Ornella Rossetto
    Dipartimento di Scienze Biomediche and Istituto CNR di Neuroscienze, Universita di Padova, Padova, Italy
    J Neurochem 97:1534-45. 2006
    ..It is possible to envisage clinical applications of the lysophospholipid/fatty acid mixture to inhibit hyperactive superficial nerve terminals...
  33. doi request reprint Imaging the cell entry of the anthrax oedema and lethal toxins with fluorescent protein chimeras
    Irene Zornetta
    Dipartimento di Scienze Biomediche dell Università di Padova and Istituto di Neuroscienze del CNR, Via G Colombo 3, 35100 Padova, Italy
    Cell Microbiol 12:1435-45. 2010
    ..This difference is attributed to a higher resistance of mCherry to unfolding. After translocation, LF disperses in the cytosol, while EF localizes on the cytosolic face of late endosomes...
  34. ncbi request reprint Stop the killer: how to inhibit the anthrax lethal factor metalloprotease
    Cesare Montecucco
    Istituto Veneto di Medicina Molecolare, Via Orus, 35121 Padua, Italy
    Trends Biochem Sci 29:282-5. 2004
    ..This will pave the way for design of novel anthrax therapeutics that are capable of inhibiting the metalloprotease activity of LF in vivo...
  35. ncbi request reprint Presynaptic receptor arrays for clostridial neurotoxins
    Cesare Montecucco
    Dipartimento di Scienze Biomediche and Istituto CNR di Neuroscienze, Università de Padova, Via G Colombo 3, 35121 Padua, Italy
    Trends Microbiol 12:442-6. 2004
  36. ncbi request reprint The Helicobacter pylori VacA cytotoxin activates RBL-2H3 cells by inducing cytosolic calcium oscillations
    Marina de Bernard
    Dipartimento di Biologia, Biomediche Sperimentali, Universita di Padova, Via G Colombo 3, Padova, Italy
    Cell Microbiol 7:191-8. 2005
    ..These observations indicate that VacA may act as a pro-inflammatory factor of H. pylori at very early stages of the innate immune response...
  37. ncbi request reprint SNARE complexes and neuroexocytosis: how many, how close?
    Cesare Montecucco
    Dipartimento di Scienze Biomediche and Istituto CNR di Neuroscienze, Universita di Padova, Viale G Colombo n 3, 35121 Padova, Italy
    Trends Biochem Sci 30:367-72. 2005
    ..In addition, we propose that the N value in neuroexocytosis can be estimated by the comparative use of different types of botulinum neurotoxins...
  38. doi request reprint Different mechanisms of inhibition of nerve terminals by botulinum and snake presynaptic neurotoxins
    Cesare Montecucco
    Dipartimento di Scienze Biomediche and Istituto CNR di Neuroscienze, Universita di Padova, Viale G Colombo 3, 35131 Padova, Italy
    Toxicon 54:561-4. 2009
    ..This difference appears evident from the beginning of intoxication, i.e. neurotoxins binding to peripheral nerve terminals and proceeds with the different site of actions and molecular targets...
  39. ncbi request reprint Peculiar binding of botulinum neurotoxins
    Ornella Rossetto
    Dipartimento di Scienze Biomediche and Istituto CNR di Neuroscienze, Universita di Padova, Viale G Colombo 3, 35121 Padova, Italy
    ACS Chem Biol 2:96-8. 2007
    ..Recent structures provide insight into how BoNT interacts with these cell surface components and open the door for the development of inhibitors against this neurotoxin...
  40. ncbi request reprint Botulinal neurotoxins: revival of an old killer
    Cesare Montecucco
    Dipartimento di Scienze Biomediche and Istituto CNR di Neuroscienze, Universita di Padova, Viale G Colombo 3, 35131 Padova, Italy
    Curr Opin Pharmacol 5:274-9. 2005
    ..Their range of clinical applications is continuously growing, and BoNT/A is being used extensively as a pharmaco-cosmetic...
  41. ncbi request reprint A lysolecithin/fatty acid mixture promotes and then blocks neurotransmitter release at the Drosophila melanogaster larval neuromuscular junction
    Aram Megighian
    Department of Human Anatomy and Physiology, Section of Physiology, University of Padova, Via Marzolo 3, Padova, Italy
    Neurosci Lett 416:6-11. 2007
    ..This novel tool could contribute to the unraveling of the molecular steps involved in neuroexocytosis, particularly in Drosophila, where it is straightforward to combine it with electrophysiology and genetics...
  42. ncbi request reprint Tyrosine-728 and glutamic acid-735 are essential for the metalloproteolytic activity of the lethal factor of Bacillus anthracis
    Fiorella Tonello
    Istituto di Neuroscienze del CNR, Universita di Padova, Via G Colombo 3, 35121 Padua, Italy
    Biochem Biophys Res Commun 313:496-502. 2004
    ..These mutants have neither proteolytic activity nor in vivo toxicity. The possible role of Tyr-728 in catalysis is discussed...
  43. ncbi request reprint Structure of the neutrophil-activating protein from Helicobacter pylori
    Giuseppe Zanotti
    Dipartimento di Chimica Organica e Centro CNR Biopolimeri, Universita di Padova, Via Marzolo 1, 35131, Padova, Italy
    J Mol Biol 323:125-30. 2002
    ..This is due to the presence of a large number of positively charged residues, which could well account for its unique ability in activating human leukocytes...
  44. ncbi request reprint A molecular model of the Vibrio cholerae cytolysin transmembrane pore
    Sergio Pantano
    Venetian Institute of Molecular Medicine VIMM, Via Orus 2, 35129, Padova, Italy
    Toxicon 47:35-40. 2006
    ..Calculations of the electrostatic profile within the pore lumen provide a rationale for the low conductance and selectivity of the VCC ion channel...
  45. ncbi request reprint Where and how do anthrax toxins exit endosomes to intoxicate host cells?
    Andrea Puhar
    Dipartimento di Scienze Biomediche Sperimentali, Universita di Padova, Viale G Colombo 3, I 35121 Padua, Italy
    Trends Microbiol 15:477-82. 2007
    ..Here, the current model of anthrax toxin cell entry, an alternative model and experimental approaches for clarifying unanswered questions will be discussed...
  46. ncbi request reprint VAMP/synaptobrevin cleavage by tetanus and botulinum neurotoxins is strongly enhanced by acidic liposomes
    Paola Caccin
    Istituto di Neuroscienze del CNR Biomembrane and Dipartimento di Scienze Biomediche, Universita di Padova, Via G Colombo 3, 35121 Padova, Italy
    FEBS Lett 542:132-6. 2003
    ..This effect is at least partially due to the binding of the metalloprotease to the lipid membranes, with electrostatic interactions playing an important role...
  47. ncbi request reprint Structure of two iron-binding proteins from Bacillus anthracis
    Elena Papinutto
    Dipartimento di Chimica Organica e Centro CNR Biopolimeri, Universita di Padova, Via Marzolo 1, 35131 Padova, Italy
    J Biol Chem 277:15093-8. 2002
    ..They are sphere-like proteins with an internal cavity. We also show that they act as ferritins and are thus involved in iron uptake and regulation, a fundamental function during bacterial growth...
  48. pmc Immunosuppressive and proinflammatory activities of the VacA toxin of Helicobacter pylori
    Cesare Montecucco
    Dipartimento di Scienze Biomediche, Universita di Padova, 35121 Padova, Italy
    J Exp Med 198:1767-71. 2003
  49. ncbi request reprint The metalloproteolytic activity of the anthrax lethal factor is substrate-inhibited
    Fiorella Tonello
    Istituto di Neuroscienze, Consiglio Nazionale delle Ricerche, Via G Colombo 3, 35121 Padova, Italy
    J Biol Chem 278:40075-8. 2003
    ..LF undergoes substrate inhibition, in keeping with the non-productive binding geometry of the MAPPK-2 N terminus to LF...
  50. doi request reprint Heat-induced transcription of diphtheria toxin A or its variants, CRM176 and CRM197: implications for pancreatic cancer gene therapy
    P Fogar
    Department of Medical and Surgical Sciences, University of Padova, Padova, Italy
    Cancer Gene Ther 17:58-68. 2010
    ....
  51. ncbi request reprint Reversible skeletal neuromuscular paralysis induced by different lysophospholipids
    Paola Caccin
    Dipartimento di Scienze Biomediche, Istituto C N R Neuroscienze, Universita di Padova, Viale G Colombo n 3, 35121 Padova, Italy
    FEBS Lett 580:6317-21. 2006
    ..This paralysis is fully reversed by albumin washing. These findings provide novel insights on the mode of action of snake neurotoxins and qualify lysoPLs as novel agents to study neuroexocytosis...
  52. pmc Anthrax edema toxin modulates PKA- and CREB-dependent signaling in two phases
    Andrea Puhar
    Dipartimento di Scienze Biomediche Sperimentali, Universita di Padova, Padova, Italy
    PLoS ONE 3:e3564. 2008
    ..The ability of EdTx to modulate cAMP-dependent signaling was studied in Jurkat T cells and was compared with that of other cAMP-rising agents: Bordetella pertussis adenylate cyclase toxin, cholera toxin and forskolin...
  53. ncbi request reprint Calcium influx and mitochondrial alterations at synapses exposed to snake neurotoxins or their phospholipid hydrolysis products
    Michela Rigoni
    Department of Biomedical Sciences and Consiglio Nazionale Ricerche Institute of Neuroscience, University of Padova, 35121 Padova, Italy
    J Biol Chem 282:11238-45. 2007
    ..These results complete the picture of events occurring at nerve terminals intoxicated by SPANs and define the LysoPC+FA lipid mixture as a novel and effective agonist of synaptic vesicle release...
  54. doi request reprint Ratio of lethal and edema factors in rabbit systemic anthrax
    Federica Dal Molin
    Dipartimento di Scienze Biomediche CNR Istituto di Neuroscienze, Viale G Colombo 3, 35121 Padova, Italy
    Toxicon 52:824-8. 2008
    ..It is therefore fundamental to know their concentration ratio in vivo. Here, we report the first determination of the concentration ratio of anthrax toxin components LF/EF in the serum of rabbits infected with B. anthracis spores...
  55. ncbi request reprint Neuromuscular paralysis and recovery in mice injected with botulinum neurotoxins A and C
    Laura Morbiato
    Dipartimento di Scienze Biomediche and Istituto CNR di Neuroscienze, Universita di Padova, Padova, Italy
    Eur J Neurosci 25:2697-704. 2007
    ..The present study shows an extensive similarity in the biological activities of BoNT/A and BoNT/C, further supporting the suggestion that BoNT/C is a valid alternative to BoNT/A...
  56. doi request reprint cAMP imaging of cells treated with pertussis toxin, cholera toxin, and anthrax edema toxin
    Federica Dal Molin
    Dipartimento di Scienze Biomediche and Istituto C N R Neuroscienze, Universita di Padova, Viale G Colombo n 3, 35121 Padova, Italy
    Biochem Biophys Res Commun 376:429-33. 2008
    ..On the contrary, CT, which acts on the plasma membrane adenylate cyclase, did not. The potential of imaging methods in studying the mode of entry and the intracellular action of bacterial toxins is discussed...
  57. ncbi request reprint Snake presynaptic neurotoxins with phospholipase A2 activity induce punctate swellings of neurites and exocytosis of synaptic vesicles
    Michela Rigoni
    Department of Biomedical Sciences and CNR Institute of Neuroscience, University of Padova, Viale G Colombo 3, 35121 Padova, Italy
    J Cell Sci 117:3561-70. 2004
    ..These changes closely resemble those occurring at neuromuscular junctions of intoxicated animals and fully qualify these primary neuronal cultures as pertinent models for studying the molecular mode of action of these neurotoxins...
  58. ncbi request reprint The Vibrio cholerae haemolysin anion channel is required for cell vacuolation and death
    Monica Moschioni
    Centro CNR Biomembrane and Dipartimento di Scienze Biomediche Sperimentali, Universita di Padova, Via G Colombo 3, 35121, Padova, Italy
    Cell Microbiol 4:397-409. 2002
    ..Using markers of cell organelles, we show that vacuoles derive from different intracellular compartments and we identify the contribution of late endosomes and of the trans-Golgi network in vacuole biogenesis...
  59. ncbi request reprint Cloning, expression, purification, and characterization of Streptococcus pneumoniae IgA1 protease
    V Romanello
    Dipartimento di Scienze Biomediche, Universita di Padova, Viale G Colombo 3, 35121 Padova, Italy
    Protein Expr Purif 45:142-9. 2006
    ..Moreover the inactive mutant could be considered as a candidate vaccine component...
  60. ncbi request reprint Determination of diphtheria toxin neutralizing antibody titers with a cell protein synthesis inhibition assay
    E Padovan
    Centro C N R Biomembrane e Istituto di Patologia Generale, Universita di Padova, Italy
    Med Microbiol Immunol 180:29-35. 1991
    ..Antitoxin titers as low as 0.002 IU can be reproducibly and accurately measured and the method gives a linear response in the range 0.002 to 8.0 IU/ml...
  61. ncbi request reprint Tetanus and botulinum-B neurotoxins block neurotransmitter release by proteolytic cleavage of synaptobrevin
    G Schiavo
    Dipartimento di Scienze Biomediche, Universita di Padova, Italy
    Nature 359:832-5. 1992
    ..These results indicate that tetanus and botulinum B neurotoxins block neurotransmitter release by cleaving synaptobrevin-2, a protein that, on the basis of our results, seems to play a key part in neurotransmitter release...
  62. ncbi request reprint The neutrophil-activating protein of Helicobacter pylori (HP-NAP) activates the MAPK pathway in human neutrophils
    Hiroaki Nishioka
    Istituto Veneto di Medicina Molecolare, Immunologia Clinica, Universita di Padova, Padova, Italy
    Eur J Immunol 33:840-9. 2003
    ..These results demonstrate that HP-NAP activates neutrophils through a PTX-sensitive pathway and that ERK and p38-MAPK are involved in many neutrophil functions stimulated by HP-NAP...
  63. pmc The Vibrio cholerae cytolysin promotes activation of mast cell (T helper 2) cytokine production
    Diletta Arcidiacono
    Department of Biomedical Sciences, University of Padua, Via G Colombo 3, 35121 Padua, Italy
    Cell Microbiol 10:899-907. 2008
    ..These findings strongly suggest that VCC has a pro-inflammatory activity promoting a Th2-type immune profile...
  64. pmc Cell entry and cAMP imaging of anthrax edema toxin
    Federica Dal Molin
    Dipartimento di Scienze Biomediche ed Istituto CNR Neuroscienze, Padova, Italy
    EMBO J 25:5405-13. 2006
    ..EF remains associated to these compartments and its activity shows a perinuclear localization generating intracellular cAMP concentration gradients from the cell centre to the periphery...
  65. ncbi request reprint The concerted action of the Helicobacter pylori cytotoxin VacA and of the v-ATPase proton pump induces swelling of isolated endosomes
    Christophe Genisset
    Dipartimento di Scienze Biomediche Sperimentali, Universita di Padova, Padova, Italy
    Cell Microbiol 9:1481-90. 2007
    ....
  66. ncbi request reprint Crystal structure of antigen TpF1 from Treponema pallidum
    Anton Thumiger
    Dipartimento di Scienze Chimiche e ICB CNR, Universita di Padova, Padova, Italy
    Proteins 62:827-30. 2006
  67. doi request reprint Calcium imaging of muscle cells treated with snake myotoxins reveals toxin synergism and presence of acceptors
    M Cintra-Francischinelli
    Dipartimento di Scienze Biomediche, Universita di Padova, Viale G Colombo, 3, 35121, Padova, Italy
    Cell Mol Life Sci 66:1718-28. 2009
    ..Therefore, these myotoxins may bind to acceptor(s) coupled to intracellular Ca(2+) mobilization in both myoblasts and myotubes. However, in myotubes only, the toxins alter plasma membrane permeability, leading to death...
  68. doi request reprint Assay of diffusion of different botulinum neurotoxin type a formulations injected in the mouse leg
    Luca Carli
    Department of Biomedical Sciences, University of Padova, Viale G Colombo 3, Padova, Italy
    Muscle Nerve 40:374-80. 2009
    ..The results presented here indicate that there is no significant difference between Botox, Dysport, and Xeomin with respect to diffusion into adjacent muscles in the mouse leg...
  69. doi request reprint The C-terminal region of a Lys49 myotoxin mediates Ca2+ influx in C2C12 myotubes
    Mariana Cintra-Francischinelli
    Dipartimento di Scienze Biomediche, Universita di Padova, Padova, Italy
    Toxicon 55:590-6. 2010
    ..These observations provide relevant insights into the mechanism of action of this family of toxins, with implications for the understanding of their structure-function relationships...
  70. doi request reprint Mass spectrometry analysis of the phospholipase A(2) activity of snake pre-synaptic neurotoxins in cultured neurons
    Marco Paoli
    Department of Biomedical Sciences and CNR Institute of Neuroscience, University of Padova, Padova, Italy
    J Neurochem 111:737-44. 2009
    ..Although these toxins were recently shown to enter neurons, their intracellular hydrolytic action and the activation of intracellular PLA(2)s appear to contribute little, if any, to the phospholipid hydrolysis measured here...
  71. ncbi request reprint The neutrophil-activating protein of Helicobacter pylori
    William G Dundon
    Centro CNR Biomembrane e Dipartimento di Scienze Biomediche, Universita di Padova, Italy
    Int J Med Microbiol 291:545-50. 2002
    ..Sequence analysis of the genomes of several other bacteria reveal that many possess Dps/HP-NAP-like proteins. The preliminary characterisation of some of these proteins will be discussed...
  72. doi request reprint The N-terminal half of the receptor domain of botulinum neurotoxin A binds to microdomains of the plasma membrane
    Lucia Muraro
    Department of Biomedical Sciences, University of Padua, Viale G Colombo 3, 35121 Padua, Italy
    Biochem Biophys Res Commun 380:76-80. 2009
    ....
  73. doi request reprint Neurotoxicity of inverted-cone shaped lipids
    Paola Caccin
    Dipartimento di Scienze Biomediche and Istituto C N R Neuroscienze, Universita di Padova, Padova, Italy
    Neurotoxicology 30:174-81. 2009
    ..These compounds were found to cause a complete, but reversible, paralysis of the nerve-hemidiaphragm preparation and to induce bulging of neurons in culture with entry of calcium from the external medium...
  74. pmc Interaction with CagF is required for translocation of CagA into the host via the Helicobacter pylori type IV secretion system
    Marc Roger Couturier
    Department of Medical Microbiology and Immunology, University of Alberta, 1 17 Medical Sciences Building, Edmonton, Alberta T6G 2R3, Canada
    Infect Immun 74:273-81. 2006
    ..Our results suggest that CagF may be a protein with unique chaperone-like function that is involved in the early steps of CagA recognition and delivery into the type IV secretion channel...
  75. ncbi request reprint Microbiology: death of a chaperone
    Cesare Montecucco
    Nature 443:511-2. 2006
  76. ncbi request reprint Glycogen synthase kinase 3 activation is essential for the snake phospholipase A2 neurotoxin-induced secretion in chromaffin cells
    Daniel Giner
    Instituto de Neurociencias, Centro Mixto CSIC Universidad Miguel Hernández, Campus de San Juan, 03550 Alicante, Spain
    Eur J Neurosci 25:2341-8. 2007
    ..These results suggest that the activation of this enzyme plays a major role in the enhancement of exocytosis of the readily releasable granules caused by PLA2 neurotoxins in neuroendocrine chromaffin cells...
  77. ncbi request reprint Anthrax toxin: a tripartite lethal combination
    Paolo Ascenzi
    Department of Biology and Interdepartmental Laboratory for Electron Microscopy, University Roma Tre, Viale G Marconi 446, 00146, Rome, Italy
    FEBS Lett 531:384-8. 2002
    ..Inhibitors targeting different steps of toxin activity have recently been developed. Anthrax toxin has also been exploited as a therapeutic agent against cancer...
  78. pmc Characterization and immunogenicity of the CagF protein of the cag pathogenicity island of Helicobacter pylori
    Anke Seydel
    Dipartimento di Scienze Biomediche Sperimentali, Università degli Studi di Padova Istituto Veneto di Medicina Molecolare, I 35121 Padua, Italy
    Infect Immun 70:6468-70. 2002
    ..pylori G27 and that the protein is always expressed with electrophoretic mobility variations among the 20 strains tested here. We have also found that natural infection with H. pylori is able to induce antibodies against CagF...
  79. ncbi request reprint Botulinum neurotoxin serotypes A and C do not affect motor units survival in humans: an electrophysiological study by motor units counting
    Roberto Eleopra
    Department of Clinical Neuroscience, Neurology Section, S Anna University Hospital, Corso Giovecca 203, Ferrara, Italy
    Clin Neurophysiol 113:1258-64. 2002
    ..Moreover, in cultured neurons 'in vitro' BoNT/C has been reported to be more toxic than BoNT/A...
  80. ncbi request reprint Equivalent effects of snake PLA2 neurotoxins and lysophospholipid-fatty acid mixtures
    Michela Rigoni
    Department of Biomedical Sciences and Consiglio Nazionale Ricerche Institute of Neuroscience, University of Padova, Italy
    Science 310:1678-80. 2005
    ..These results draw attention to the possible role of local lipid changes in synaptic vesicle release and provide new tools for the study of exocytosis...
  81. doi request reprint Bacillus anthracis: balancing innocent research with dual-use potential
    Michael J Hudson
    Health Protection Agency, Porton Down, Salisbury, UK
    Int J Med Microbiol 298:345-64. 2008
    ..The proposed requirement for a code of conduct for working with dual-use agents such as the anthrax bacillus is also discussed...
  82. pmc Anthrax toxins suppress T lymphocyte activation by disrupting antigen receptor signaling
    Silvia Rossi Paccani
    Department of Evolutionary Biology, Policlinico Le Scotte, University of Siena, 53100 Siena, Italy
    J Exp Med 201:325-31. 2005
    ..These data identify a novel strategy of immune evasion by B. anthracis, based on both effector subunits of the toxic complex, and targeted to a key cellular component of adaptive immunity...
  83. ncbi request reprint Anthrax edema toxin cooperates with lethal toxin to impair cytokine secretion during infection of dendritic cells
    Jean Nicolas Tournier
    Département de Biologie des Agents Transmissibles, Centre de Recherches du Service de Sante des Armees, La Tronche Cedex, France
    J Immunol 174:4934-41. 2005
    ..These results suggest that ET produced during infection impairs DC functions and cooperates with LT to suppress the innate immune response. This may represent a new strategy developed by B. anthracis to escape the host immune response...
  84. ncbi request reprint VacA and HP-NAP, Ying and Yang of Helicobacter pylori-associated gastric inflammation
    Cesare Montecucco
    Department of Internal Medicine, University of Florence, Florence, Italy
    Clin Chim Acta 381:32-8. 2007
    ..Altogether these evidences indicate that both VacA and HP-NAP play a major role in generating and maintaining the gastric inflammatory response associated with the H. pylori infection...
  85. pmc Entering neurons: botulinum toxins and synaptic vesicle recycling
    Claudia Verderio
    Department of Pharmacology and CNR Institute of Neuroscience, University of Milano, Via Vanvitelli 32, 20129 Milano, Italy
    EMBO Rep 7:995-9. 2006
    ....
  86. ncbi request reprint Traffic of botulinum toxins A and E in excitatory and inhibitory neurons
    Claudia Verderio
    Department of Medical Pharmacology, University of Milano, Via Vanvitelli 32, 20129 Milano, Italy
    Traffic 8:142-53. 2007
    ....
  87. ncbi request reprint The neutrophil-activating protein of Helicobacter pylori crosses endothelia to promote neutrophil adhesion in vivo
    Alessandra Polenghi
    Department of Biology, University of Padua, Padua, Italy
    J Immunol 178:1312-20. 2007
    ..pylori infection...
  88. ncbi request reprint Anthrax toxins inhibit immune cell chemotaxis by perturbing chemokine receptor signalling
    Silvia Rossi Paccani
    Department of Evolutionary Biology, University of Siena, Via Aldo Moro 2, 53100 Siena, Italy
    Cell Microbiol 9:924-9. 2007
    ..The data highlight a novel strategy of immunosuppression by B. anthracis based on inhibition of immune cell homing...
  89. ncbi request reprint Antiepileptic effects of botulinum neurotoxin E
    Laura Costantin
    Scuola Normale Superiore, Consiglio Nazionale delle Ricerche, 56100 Pisa, Italy
    J Neurosci 25:1943-51. 2005
    ..We conclude that BoNT/E delivery to the hippocampus is both antiictal and antiepileptogenic in experimental models of epilepsy...
  90. ncbi request reprint Internalization and mechanism of action of clostridial toxins in neurons
    Carlotta Grumelli
    Department of Medical Pharmacology and CNR Institute of Neuroscience, Center of Excellence for Neurodegenerative Diseases, University of Milano, Via Vanvitelli 32, 20129 Milano, Italy
    Neurotoxicology 26:761-7. 2005
    ..Their use in cell biology and neuroscience is considered, as well as their therapeutic utilization in human diseases characterized by hyperfunction of cholinergic terminals...
  91. ncbi request reprint Streptococcus pneumoniae induces mast cell degranulation
    Giovanna Barbuti
    Dipartimento di Scienze Biomediche e Oncologia Umana, Sezione di Patologia Generale, Universita di Bari, P zza Giulio Cesare 11, I 70124 Bari, Italy
    Int J Med Microbiol 296:325-9. 2006
    ....
  92. ncbi request reprint Taipoxin induces F-actin fragmentation and enhances release of catecholamines in bovine chromaffin cells
    Patricia Neco
    Instituto de Neurociencias, Centro Mixto CSIC Universidad Miguel Hernández, Alicante, Spain
    J Neurochem 85:329-37. 2003
    ..Taken together, these results suggest that taipoxin causes F-actin fragmentation and enhances secretion by redistribution of vesicles among secretory pools...
  93. ncbi request reprint Anthrax toxins: A paradigm of bacterial immune suppression
    Cosima T Baldari
    Department of Evolutionary Biology, University of Siena, 53100, Siena, Italy
    Trends Immunol 27:434-40. 2006
    ..anthracis-specific responses. Thus, B. anthracis can invade the host, with ensuing massive bacteremia and toxemia. Here, we review the specific effects of B. anthracis on neutrophils, macrophages, dendritic cells, T- and B-lymphocytes...
  94. ncbi request reprint Botulinum neurotoxins and formalin-induced pain: central vs. peripheral effects in mice
    Siro Luvisetto
    Istituto di Neuroscienze del CNR, Psicobiologia e Psicofarmacologia, Centro Europeo di Ricerca sul Cervello, Via del Fosso di Fiorano 64, 00143 Roma, Italy
    Brain Res 1082:124-31. 2006
    ..Such data suggest BoNTs as a useful tool in the studies aimed at the comprehension of the mechanisms of inflammatory pain...
  95. ncbi request reprint Taipoxin induces synaptic vesicle exocytosis and disrupts the interaction of synaptophysin I with VAMP2
    Dario Bonanomi
    Department of Neuroscience, San Raffaele Scientific Institute, Via Olgettina 58, 20132 Milan, Italy
    Mol Pharmacol 67:1901-8. 2005
    ..These early effects of the toxin are followed by severe impairment of SV exo-endocytosis, which might underlie the prevention of neurotransmitter release reported after intoxication by taipoxin...
  96. ncbi request reprint Transient synaptic silencing of developing striate cortex has persistent effects on visual function and plasticity
    Matteo Caleo
    Istituto di Neuroscienze, Consiglio Nazionale delle Ricerche, 56100 Pisa, Italy
    J Neurosci 27:4530-40. 2007
    ..Unexpectedly, these effects extended equally to the contralateral, uninjected side, demonstrating a fundamental role for interhemispheric connections in cortical maturation...
  97. pmc G-CSF-stimulated neutrophils are a prominent source of functional BLyS
    Patrizia Scapini
    Department of Pathology, University of Verona, 37134 Verona, Italy
    J Exp Med 197:297-302. 2003
    ..Collectively, our findings suggest that neutrophils, through the production of BLyS, might play an unsuspected role in the regulation of B cell homeostasis...
  98. ncbi request reprint Toxicity of botulinum neurotoxins in central nervous system of mice
    Siro Luvisetto
    Istituto di Neuroscienze del CNR, Sez di Psicobiologia e Psicofarmacologia, V le Marx 15 43, 00137 Roma, Italy
    Toxicon 41:475-81. 2003
    ..The surviving mice recovered completely within 4-6 days and regained the initial healthy conditions. At sub-lethal doses, the same clinical signs appear in a lighter form and with a longer time course...
  99. doi request reprint On the quaternary structure of taipoxin and textilotoxin: the advantage of being multiple
    Cesare Montecucco
    Toxicon 51:1560-2. 2008
  100. ncbi request reprint Different types of botulinum toxin in humans
    Roberto Eleopra
    Department of Clinical Neurosciences, S Anna University Hospital, Ferrara, Italy
    Mov Disord 19:S53-9. 2004
    ..BoNT/F might be useful when only a short duration of neuromuscular blockade is required...
  101. pmc Suppression of T-lymphocyte activation and chemotaxis by the adenylate cyclase toxin of Bordetella pertussis
    Silvia Rossi Paccani
    Department of Evolutionary Biology, University of Siena, Via Aldo Moro 2, 53100 Siena, Italy
    Infect Immun 76:2822-32. 2008
    ....