Research Topics
| Marina LottiSummaryAffiliation: University of Milano-Bicocca Country: Italy Publications
| Collaborators
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Detail Information
Publications
Mutual effects of disorder and order in fusion proteins between intrinsically disordered domains and fluorescent proteinsMarina Lotti
Dipartimento di Biotecnologie e Bioscienze, Università Statale Milano Bicocca, Milan, Italy
Mol Biosyst 8:105-13. 2012..The impact of the artificial fusion on the conformational and functional properties of the resulting proteins is discussed...- Evolution of stability in a cold-active enzyme elicits specificity relaxation and highlights substrate-related effects on temperature adaptationPietro Gatti-Lafranconi
Department of Biotechnology and Biosciences, State University of Milano Bicocca, Piazza della Scienza 2, I 20126 Milano, Italy
J Mol Biol 395:155-66. 2010..This reduction of lid movements is suggested to be accompanied by a concomitant increase in the mobility of other protein regions, thus accounting for the observed broadening of substrate specificity... - How disorder influences order and vice versa--mutual effects in fusion proteins containing an intrinsically disordered and a globular proteinIlaria Sambi
Dipartimento di Biotecnologie e Bioscienze, Universita di Milano Bicocca, Milano, Italy
FEBS J 277:4438-51. 2010.... 
Compaction properties of an intrinsically disordered protein: Sic1 and its kinase-inhibitor domainStefania Brocca
Department of Biotechnology and Biosciences, University of Milano Bicocca, Milan, Italy
Biophys J 100:2243-52. 2011..Comparison between the fragment and the full-length protein suggests that chain length is crucial to the stabilization of compact states of this IDP. The two proteins are compared by a length-independent compaction index...- In vivo aggregation of bovine beta-lactoglobulin is affected by Cys at position 121Gaetano Invernizzi
Dipartimento di Biotecnologie e Bioscienze, Universita degli Studi di Milano Bicocca, Milano, Italy
Protein Expr Purif 62:111-5. 2008..Our results underline the key contribution of the unpaired cysteine residue during the oxidative folding pathway and indicate BLG as a useful tool for the study of protein aggregation in vivo... - Fourier transform infrared spectroscopy analysis of the conformational quality of recombinant proteins within inclusion bodiesSilvia Maria Doglia
Dipartimento di Biotecnologie e Bioscienze, Universita degli Studi di Milano Bicocca, Milano, Italia
Biotechnol J 3:193-201. 2008..Examples are reported concerning the study of kinetics of aggregation and structure of aggregates as a function of expression levels, temperature and co-expression of chaperones... - Defining structural domains of an intrinsically disordered protein: Sic1, the cyclin-dependent kinase inhibitor of Saccharomyces cerevisiaeStefania Brocca
Department of Biotechnology and Biosciences, Universita di Milano Bicocca, P zza della Scienza 2, 20126, Milan, Italy
Mol Biotechnol 47:34-42. 2011..Circular dichroism (CD) measurements and nuclear magnetic resonance (NMR) spectra of N- and C-terminal fragments confirm their disordered nature but reveal minor structural differences that may reflect their distinct functional roles... 
Kinetics of inclusion body formation studied in intact cells by FT-IR spectroscopyDiletta Ami
Dipartimento di Biotecnologie e Bioscienze, , Piazza della Scienza 2, 20126 Milan, Italy
FEBS Lett 579:3433-6. 2005..Furthermore, insights on the residual native-like structure of the expressed protein within IB--both isolated and inside cells--were obtained by the secondary structure analysis of the Amide I band in the IB FT-IR spectra...- Comparison of bovine and porcine beta-lactoglobulin: a mass spectrometric analysisGaetano Invernizzi
Department of Biotechnology and Biosciences, University Milano-Bicocca, Piazza della Scienza 2, 20126 Milan, Italy
J Mass Spectrom 41:717-27. 2006..Equilibrium folding intermediates of both proteins are detected. Finally, conditions are found that promote dissociation of the BLG dimer at pH 6 into folded monomers... - Laboratory evolution of copper tolerant yeast strainsGiusy Manuela Adamo
Dipartimento di Biotecnologie e Bioscienze, Universita degli Studi di Milano Bicocca, Piazza della Scienza 2, 20126 Milano, Italy
Microb Cell Fact 11:1. 2012..Moreover, they can contribute to the study of human diseases related to impairments of copper metabolism. In this study, we investigated the molecular and physiological factors that confer copper tolerance to strains of baker's yeasts... - Relevance of metal ions for lipase stability: structural rearrangements induced in the Burkholderia glumae lipase by calcium depletionGaetano Invernizzi
Dipartimento di Biotecnologie e Bioscienze, Universita degli Studi di Milano Bicocca, Milano, Italy
J Struct Biol 168:562-70. 2009.... - Unscrambling thermal stability and temperature adaptation in evolved variants of a cold-active lipasePietro Gatti-Lafranconi
Dipartimento di Biotecnologie e Bioscienze, Universita degli Studi di Milano Bicocca, Piazza della Scienza 2, Milan, Italy
FEBS Lett 582:2313-8. 2008..Biophysical measurements did not indicate any obvious difference between the improved variant and the wild type enzyme in terms of loss of secondary structure upon heat treatment, nor a shift in the apparent melting temperature... - Deactivation and unfolding are uncoupled in a bacterial lipase exposed to heat, low pH and organic solventsGaetano Invernizzi
Dipartimento di Biotecnologie e Bioscienze, Universita degli Studi di Milano Bicocca, Milano, Italy
J Biotechnol 141:42-6. 2009..In particular, no denaturation at all could be elicited by dimethyl formamide (DMF), isopropanol, and dimethyl sulfoxide (DMSO) up to 80%, in spite of a reduction of enzyme activity to 60-75%... - Electrospray-ionization mass spectrometry as a tool for fast screening of protein structural propertiesRita Grandori
Dipartimento di Biotecnologie e Bioscienze, Universita degli Studi di Milano Bicocca, Milan, Italy
Biotechnol J 4:73-87. 2009.... - Promiscuity, stability and cold adaptation of a newly isolated acylaminoacyl peptidaseElectra A S Brunialti
Dipartimento di Biotecnologie e Bioscienze, Universita di Milano Bicocca, 20126 Milano, Italy
Biochimie 93:1543-54. 2011..2 × 10(3) s(-1) M(-1), respectively. Despite some properties cannot be explained with current models, results report on the relevance of structural and catalytic properties for the successful adaptation to cold temperatures... - Secondary structure, conformational stability and glycosylation of a recombinant Candida rugosa lipase studied by Fourier-transform infrared spectroscopyAntonino Natalello
Dipartimento di Biotecnologie e Bioscienze, , Piazza della Scienza 2, 20126 Milano, Italy
Biochem J 385:511-7. 2005..Glycosidic structures of the high mannose type were found, with mannoses ranging from 8 to 25 residues... - Plasma-induced graft-polymerization of polyethylene glycol acrylate on polypropylene films: chemical characterization and evaluation of the protein adsorptionStefano Zanini
Dipartimento di Fisica G Occhialini, Universita degli Studi di Milano Bicocca, p za della Scienza, 3 I 20126 Milano, Italy
J Colloid Interface Sci 341:53-8. 2010.... - Order propensity of an intrinsically disordered protein, the cyclin-dependent-kinase inhibitor Sic1Stefania Brocca
Department of Biotechnology and Biosciences, University of Milano Bicocca, Milan, Italy
Proteins 76:731-46. 2009..The mutations S201A and S201E, which are known to affect Sic1 function, do not have significant effects on the conformational properties of the pure protein... - Mutations in the "lid" region affect chain length specificity and thermostability of a Pseudomonas fragi lipaseGianluca Santarossa
Dipartimento di Biotecnologie e Bioscienze, Universita degli Studi di Milano Bicocca, Italy
FEBS Lett 579:2383-6. 2005..Moreover, mutations conferred to PFL higher temperature stability. On the other hand, replacement of the serine at position 141 by glycine destabilized the protein... - Heterologous expression of bovine and porcine beta-lactoglobulins in Pichia pastoris: towards a comparative functional characterisationGaetano Invernizzi
Dipartimento di Biotecnologie e Bioscienze, , Piazza della Scienza 2, Milan, Italy
J Biotechnol 109:169-78. 2004..Recombinant lipocalins were purified by ion-exchange chromatography from the culture medium. A preliminary NMR characterisation showed that both proteins were correctly folded... - Sequence of the lid affects activity and specificity of Candida rugosa lipase isoenzymesStefania Brocca
Dipartimento di Biotecnologie e Bioscienze, Universita' degli Studi di Milano-Bicocca, 20126 Milano, Italy
Protein Sci 12:2312-9. 2003..On the other hand, a specific shift in the chain-length specificity was not observed. Chimeric proteins displayed different sensitivity to detergents in the reaction medium... - Monitoring the transport of recombinant Candida rugosa lipase by a green fluorescent protein-lipase fusionSimone Passolunghi
Dipartimento di Biotecnologie e Bioscienze, Universita' degli Studi di Milano-Bicocca, Piazza della Scienza 2, 20126 Milano, Italy
Biotechnol Lett 25:1945-8. 2003..This analytical tool enables a rapid monitoring of product localization and amount, based on GFP-associated fluorescence... - Components of the E. coli envelope are affected by and can react to protein over-production in the cytoplasmRiccardo Villa
Dipartimento di Biotecnologie e Bioscienze, Universita degli Studi di Milano Bicocca, Piazza della Scienza 2, Milano, Italy
Microb Cell Fact 8:32. 2009..abstract:..