Giorgio Lenaz

Summary

Affiliation: University of Bologna
Country: Italy

Publications

  1. ncbi request reprint Plasma membrane oxidoreductase activity in cultured cells in relation to mitochondrial function and oxidative stress
    Giulia Deleonardi
    Dipartimento di Biochimica, Universita di Bologna, Via Irnerio 48, 40126 Bologna, Italy
    Biofactors 20:251-8. 2004
  2. ncbi request reprint Ubiquinol and a coenzyme Q reducing system protect platelet mitochondrial function of transfusional buffy coats from oxidative stress
    Milena Merlo Pich
    Dipartimento di Biochimica G Moruzzi, Universita di Bologna, Italy
    Free Radic Res 36:429-36. 2002
  3. ncbi request reprint Oxidation of glycerol-3-phosphate in porcine and bovineadrenal cortex mitochondria
    Jerzy Popinigis
    Department of Bioenergetics, Jedrzej Sniadecki University School of Physical Education and Sport, Gdansk, Poland
    Acta Biochim Pol 51:1075-80. 2004
  4. ncbi request reprint Mitochondrial activities of a cell line derived from thyroid Hürthle cell tumors
    Karmen Stankov
    Dipartimento di Medicina Interna, Cardioangiologia ed Epatologia, Unita Operativa di Genetica Medica, Policlinico S Orsola Malpighi, Bologna, Italy
    Thyroid 16:325-31. 2006
  5. ncbi request reprint The study of the pathogenic mechanism of mitochondrial diseases provides information on basic bioenergetics
    Giancarlo Solaini
    Dipartimento di Biochimica G Moruzzi, Via Irnerio, 48, Universita di Bologna, Italy
    Biochim Biophys Acta 1777:941-5. 2008
  6. ncbi request reprint Role of mitochondria in oxidative stress and aging
    Giorgio Lenaz
    Dipartimento di Biochimica G Moruzzi, Universita di Bologna, Via Irnerio 48, 40126 Bologna, Italy
    Ann N Y Acad Sci 959:199-213. 2002
  7. ncbi request reprint Enhanced activity of the plasma membrane oxidoreductase in circulating lymphocytes from insulin-dependent diabetes mellitus patients
    Giorgio Lenaz
    Dipartimento di Biochimica, Universita di Bologna, Via Irnerio 48, Bologna 40126, Italy
    Biochem Biophys Res Commun 290:1589-92. 2002
  8. ncbi request reprint The mitochondrial production of reactive oxygen species: mechanisms and implications in human pathology
    G Lenaz
    Dipartimento di Biochimica G Moruzzi, University of Bologna, Italy
    IUBMB Life 52:159-64. 2001
  9. ncbi request reprint A critical appraisal of the mitochondrial coenzyme Q pool
    G Lenaz
    Dipartimento di Biochimica, Universita di Bologna, Via Irnerio 48, 40126, Bologna, Italy
    FEBS Lett 509:151-5. 2001
  10. ncbi request reprint Mitochondrial Complex I: structural and functional aspects
    Giorgio Lenaz
    Department of Biochemistry, University of Bologna, Via Irnerio 48, 40126 Bologna, Italy
    Biochim Biophys Acta 1757:1406-20. 2006

Detail Information

Publications88

  1. ncbi request reprint Plasma membrane oxidoreductase activity in cultured cells in relation to mitochondrial function and oxidative stress
    Giulia Deleonardi
    Dipartimento di Biochimica, Universita di Bologna, Via Irnerio 48, 40126 Bologna, Italy
    Biofactors 20:251-8. 2004
    ..The results suggest that enhanced oxidative stress and not deficient respiratory activity per se is the stimulus triggering over-expression of plasma membrane oxidative enzymes...
  2. ncbi request reprint Ubiquinol and a coenzyme Q reducing system protect platelet mitochondrial function of transfusional buffy coats from oxidative stress
    Milena Merlo Pich
    Dipartimento di Biochimica G Moruzzi, Universita di Bologna, Italy
    Free Radic Res 36:429-36. 2002
    ....
  3. ncbi request reprint Oxidation of glycerol-3-phosphate in porcine and bovineadrenal cortex mitochondria
    Jerzy Popinigis
    Department of Bioenergetics, Jedrzej Sniadecki University School of Physical Education and Sport, Gdansk, Poland
    Acta Biochim Pol 51:1075-80. 2004
    ....
  4. ncbi request reprint Mitochondrial activities of a cell line derived from thyroid Hürthle cell tumors
    Karmen Stankov
    Dipartimento di Medicina Interna, Cardioangiologia ed Epatologia, Unita Operativa di Genetica Medica, Policlinico S Orsola Malpighi, Bologna, Italy
    Thyroid 16:325-31. 2006
    ..We conducted a study on the functional characteristics of mitochondria in an oxyphilic thyroid tumor cell line, which may provide useful clues about Hürthle cell tumors carcinogenesis...
  5. ncbi request reprint The study of the pathogenic mechanism of mitochondrial diseases provides information on basic bioenergetics
    Giancarlo Solaini
    Dipartimento di Biochimica G Moruzzi, Via Irnerio, 48, Universita di Bologna, Italy
    Biochim Biophys Acta 1777:941-5. 2008
    ..We propose that the integrity of transmembrane helix III is essential for the mechanical function of ATPase 6 as a stator element in the ATP synthase, but that it is not relevant for oligomycin inhibition...
  6. ncbi request reprint Role of mitochondria in oxidative stress and aging
    Giorgio Lenaz
    Dipartimento di Biochimica G Moruzzi, Universita di Bologna, Via Irnerio 48, 40126 Bologna, Italy
    Ann N Y Acad Sci 959:199-213. 2002
    ..The plasma membrane oxidoreductase and DT-diaphorase are two such systems: likewise, they are overexpressed under oxidative stress conditions...
  7. ncbi request reprint Enhanced activity of the plasma membrane oxidoreductase in circulating lymphocytes from insulin-dependent diabetes mellitus patients
    Giorgio Lenaz
    Dipartimento di Biochimica, Universita di Bologna, Via Irnerio 48, Bologna 40126, Italy
    Biochem Biophys Res Commun 290:1589-92. 2002
    ..The increase of PMOR in diabetes is likely due to overexpression of the enzyme, in view of the very low K(m) for NADH indicating that, in intact cells, the enzyme is practically saturated with the reductant substrate...
  8. ncbi request reprint The mitochondrial production of reactive oxygen species: mechanisms and implications in human pathology
    G Lenaz
    Dipartimento di Biochimica G Moruzzi, University of Bologna, Italy
    IUBMB Life 52:159-64. 2001
    ..Accordingly, defects inducing decrease of electron transfer in the respiratory chain, as in many pathological conditions, are bound to enhance ROS production...
  9. ncbi request reprint A critical appraisal of the mitochondrial coenzyme Q pool
    G Lenaz
    Dipartimento di Biochimica, Universita di Bologna, Via Irnerio 48, 40126, Bologna, Italy
    FEBS Lett 509:151-5. 2001
    ....
  10. ncbi request reprint Mitochondrial Complex I: structural and functional aspects
    Giorgio Lenaz
    Department of Biochemistry, University of Bologna, Via Irnerio 48, 40126 Bologna, Italy
    Biochim Biophys Acta 1757:1406-20. 2006
    ..The implications in human pathology of better knowledge on these aspects of Complex I structure and function are briefly discussed...
  11. ncbi request reprint Mitochondrial bioenergetics in aging
    G Lenaz
    Dipartimento di Biochimica G Moruzzi, Universita di Bologna, Via Irnerio 48, 40126, Bologna, Italy
    Biochim Biophys Acta 1459:397-404. 2000
    ..Exogenous coenzyme Q, however, protects cells from oxidative stress by conversion into its reduced antioxidant form by cellular reductases...
  12. ncbi request reprint Kinetics of integrated electron transfer in the mitochondrial respiratory chain: random collisions vs. solid state electron channeling
    Giorgio Lenaz
    Dipartimento di Biochimica G Moruzzi, Via Irnerio 48, 40126 Bologna, Italy
    Am J Physiol Cell Physiol 292:C1221-39. 2007
    ....
  13. ncbi request reprint Mitochondria, oxidative stress, and antioxidant defences
    G Lenaz
    Dipartimento di Biochimica G Moruzzi, Universita di Bologna, Italy
    Acta Biochim Pol 46:1-21. 1999
    ..Cells counteract oxidative stress by antioxidants; among lipophilic antioxidants coenzyme Q is the only one of endogenous biosynthesis; exogenous coenzyme Q, however, may protect cells from oxidative stress in vivo...
  14. ncbi request reprint Localization and mobility of coenzyme Q in lipid bilayers and membranes
    G Lenaz
    Dipartimento di Biochimica G Moruzzi, Universita di Bologna, Italy
    Biofactors 9:87-93. 1999
    ..Since the experimental turnovers are independent of the distance between complexes, we conclude that CoQ diffusion is not rate-limiting for electron transfer...
  15. ncbi request reprint The role of Coenzyme Q in mitochondrial electron transport
    Giorgio Lenaz
    Dipartimento di Biochimica, Universita di Bologna, Via Irnerio 48, 40126 Bologna, Italy
    Mitochondrion 7:S8-33. 2007
    ..metabolic channeling, respectively, both in physiological and in some pathological conditions. As an example of intra-complex electron transfer, we discuss in particular Complex I, a topic that is still under active investigation...
  16. ncbi request reprint Oxidative stress, antioxidant defences and aging
    G Lenaz
    Dipartimento di Biochimica G Moruzzi, Universita di Bologna, Italy
    Biofactors 8:195-204. 1998
    ..The lack of the 5 kb deletion in platelets throws doubts on mitochondrial DNA lesions as the only causes of mitochondrial dysfunction in aging...
  17. ncbi request reprint Role of mitochondria in oxidative stress and ageing
    G Lenaz
    Dipartimento di Biochimica, Universita di Bologna, Via Irnerio 48, 40126 Bologna, Italy
    Biochim Biophys Acta 1366:53-67. 1998
    ....
  18. ncbi request reprint Quinone specificity of complex I
    G Lenaz
    Dipartimento di Biochimica G Moruzzi, University of Bologna, Via Irnerio 48, 40126 Bologna, Italy
    Biochim Biophys Acta 1364:207-21. 1998
    ....
  19. ncbi request reprint Mitochondrial Complex I: structure, function, and implications in neurodegeneration
    Giorgio Lenaz
    Dipartimento di Biochimica G Moruzzi, Universita di Bologna, Italy
    Ital J Biochem 55:232-53. 2006
    ....
  20. ncbi request reprint New insights into structure and function of mitochondria and their role in aging and disease
    Giorgio Lenaz
    Dipartimento di Biochimica, Universita di Bologna, Bologna, Italy
    Antioxid Redox Signal 8:417-37. 2006
    ..Evidence is presented favoring the mitochondrial theory, with primary mitochondrial alterations, although the problem is made more complex by changes in the cross-talk between nuclear and mitochondrial DNA...
  21. ncbi request reprint The mitochondrial production of reactive oxygen species in relation to aging and pathology
    Maria Luisa Genova
    Dipartimento di Biochimica G Moruzzi, University of Bologna, 40126 Bologna, Italy
    Ann N Y Acad Sci 1011:86-100. 2004
    ....
  22. doi request reprint Polyamine conjugation of curcumin analogues toward the discovery of mitochondria-directed neuroprotective agents
    Elena Simoni
    Department of Pharmaceutical Sciences, University of Bologna, Via Belmeloro 6, 40126 Bologna, Italy
    J Med Chem 53:7264-8. 2010
    ..It also resulted in a significant decrease in the cytotoxicity effects. 2-5 are therefore promising molecules for neuroprotectant lead discovery...
  23. doi request reprint Toward a rational design of multitarget-directed antioxidants: merging memoquin and lipoic acid molecular frameworks
    Maria Laura Bolognesi
    Department of Pharmaceutical Sciences, University of Bologna, Via Belmeloro 6, 40126 Bologna, Italy
    J Med Chem 52:7883-6. 2009
    ..They also displayed a balanced inhibitory profile against amyloid-beta aggregation and acetylcholinesterase, emerging as promising molecules for neuroprotectant lead discovery...
  24. ncbi request reprint The mitochondrial respiratory chain is partially organized in a supercomplex assembly: kinetic evidence using flux control analysis
    Cristina Bianchi
    Dipartimento di Biochimica G Moruzzi, Universita di Bologna, Via Irnerio 48, 40126 Bologna, Italy
    J Biol Chem 279:36562-9. 2004
    ..Moreover, we show that Complex II is fully rate-limiting for succinate oxidation, clearly indicating the absence of substrate channeling toward Complexes III and IV...
  25. ncbi request reprint Mitochondrial respiratory chain super-complex I-III in physiology and pathology
    Giorgio Lenaz
    Dipartimento di Biochimica, Universita di Bologna, Via Irnerio 48, 40126 Bologna, Italy
    Biochim Biophys Acta 1797:633-40. 2010
    ..There is increasing evidence that disruption of the super-complex organization leads to functional derangements responsible for pathological changes, as we have found in K-ras-transformed fibroblasts...
  26. ncbi request reprint Potential antitumor agents. 37. Synthesis and antitumor activity of guanylhydrazones from imidazo[2,1-b]thiazoles and from the new heterocyclic system thiazolo[2',3':2,3]imidazo[4,5-c]quinoline
    Aldo Andreani
    Dipartimento di Scienze Farmaceutiche, Universita di Bologna, Via Belmeloro 6, 40126 Bologna, Italy
    J Med Chem 48:3085-9. 2005
    ....
  27. ncbi request reprint Mitochondrial production of oxygen radical species and the role of Coenzyme Q as an antioxidant
    Maria Luisa Genova
    Dipartimento di Biochimica G Moruzzi, University of Bologna, 40126 Bologna, Italy
    Exp Biol Med (Maywood) 228:506-13. 2003
    ..The plasma membrane oxidoreductase and DT-diaphorase are two such systems, likewise, they are overexpressed under oxidative stress conditions...
  28. ncbi request reprint Structural and functional organization of Complex I in the mitochondrial respiratory chain
    Cristina Bianchi
    Universtiy of Bologna, Bologna, Italy
    Biofactors 18:3-9. 2003
    ....
  29. doi request reprint Mitochondrial Complex I decrease is responsible for bioenergetic dysfunction in K-ras transformed cells
    Alessandra Baracca
    Department of Biochemistry G Moruzzi, University of Bologna, Bologna, Italy
    Biochim Biophys Acta 1797:314-23. 2010
    ..Taken together, our results provide the new insight that the reduction of respiration observed in K-ras transformed cells is specifically due to a Complex I activity decrease...
  30. ncbi request reprint Biochemical phenotypes associated with the mitochondrial ATP6 gene mutations at nt8993
    Alessandra Baracca
    Dipartimento di Biochimica G Moruzzi, Via Irnerio 48, Universita di Bologna, 40126 Bologna, and Fondazione Ospedale Maggiore IRCCS Centro Dino Ferrari, Milano, Italy
    Biochim Biophys Acta 1767:913-9. 2007
    ....
  31. ncbi request reprint Supercomplex organization of the mitochondrial respiratory chain and the role of the Coenzyme Q pool: pathophysiological implications
    Maria Luisa Genova
    Dipartimento di Biochimica G Moruzzi, Universita di Bologna, Italy
    Biofactors 25:5-20. 2005
    ..This finding has pathophysiological implications, in view of the role of oxidative stress in the pathogenesis of many diseases...
  32. ncbi request reprint Ischemic preconditioning preserves proton leakage from mitochondrial membranes but not oxidative phosphorylation during heart reperfusion
    Claudio Muscari
    Department of Biochemistry G Moruzzi, University of Bologna, Via Irnerio 48, 40126 Bologna, Italy
    Cell Biochem Funct 24:511-8. 2006
    ..Mitochondria can contribute to cell survival by the attenuation of proton leak from inner membrane...
  33. ncbi request reprint Bioenergetics of mitochondrial diseases associated with mtDNA mutations
    Giorgio Lenaz
    Dipartimento di Biochimica G Moruzzi, Universita di Bologna, Via Irnerio 48, Bologna 40126, Italy
    Biochim Biophys Acta 1658:89-94. 2004
    ....
  34. doi request reprint Human NARP mitochondrial mutation metabolism corrected with alpha-ketoglutarate/aspartate: a potential new therapy
    Gianluca Sgarbi
    Dipartimento di Biochimica G Moruzzi, Universita di Bologna, Bologna, Italy
    Arch Neurol 66:951-7. 2009
    ....
  35. pmc Differential effects of mitochondrial Complex I inhibitors on production of reactive oxygen species
    Romana Fato
    Dipartimento di Biochimica G Moruzzi, University of Bologna, Via Irnerio 48, 40126 Bologna, Italy
    Biochim Biophys Acta 1787:384-92. 2009
    ..The results suggest that electron transfer from the iron-sulphur clusters (N2) to Coenzyme Q occurs in two steps gated by two different conformations, the former being sensitive to Rotenone and the latter to Stigmatellin...
  36. doi request reprint Structure and organization of mitochondrial respiratory complexes: a new understanding of an old subject
    Giorgio Lenaz
    Dipartimento di Biochimica G Moruzzi, Alma Mater Studiorum, Universita di Bologna, Bologna, Italy
    Antioxid Redox Signal 12:961-1008. 2010
    ..Disruption of supercomplex organization may lead to functional derangements responsible for pathologic changes...
  37. ncbi request reprint Control of oxidative phosphorylation by Complex I in rat liver mitochondria: implications for aging
    Barbara Ventura
    Dipartimento di Biochimica G Moruzzi, Universita di Bologna, Via Irnerio 48, 40126, Bologna, Italy
    Biochim Biophys Acta 1553:249-60. 2002
    ..Our results point out that age-related alterations of the mitochondrial functions are also present in tissues considered less prone to accumulate mitochondrial DNA mutations...
  38. pmc A water soluble CoQ10 formulation improves intracellular distribution and promotes mitochondrial respiration in cultured cells
    Christian Bergamini
    Department of Biochemistry G Moruzzi, University of Bologna, Bologna, Italy
    PLoS ONE 7:e33712. 2012
    ..Controversial clinical and in vitro results are mainly due to the high hydrophobicity of this compound, which reduces its bioavailability...
  39. doi request reprint New developments on the functions of coenzyme Q in mitochondria
    Maria Luisa Genova
    Dipartimento di Biochimica G Moruzzi, Universita di Bologna, Italy
    Biofactors 37:330-54. 2011
    ..Finally, a working hypothesis is advanced on the establishment of a vicious circle of oxidative stress and supercomplex disorganization in pathological states, as in neurodegeneration and cancer...
  40. ncbi request reprint Bioenergetics shapes cellular death pathways in Leber's hereditary optic neuropathy: a model of mitochondrial neurodegeneration
    Valerio Carelli
    Dipartimento di Scienze Neurologiche, Universita di Bologna, Via Ugo Foscolo 7, 40123 Bologna, Italy
    Biochim Biophys Acta 1658:172-9. 2004
    ....
  41. ncbi request reprint Mitochondrial production of reactive oxygen species: role of complex I and quinone analogues
    Romana Fato
    Dipartimento di Biochimica G Moruzzi, University of Bologna, 40126 Bologna, Italy
    Biofactors 32:31-9. 2008
    ....
  42. ncbi request reprint Effects of new ubiquinone-imidazo[2,1-b]thiazoles on mitochondrial complex I (NADH-ubiquinone reductase) and on mitochondrial permeability transition pore
    Aldo Andreani
    Dipartimento di Scienze Farmaceutiche, Universita di Bologna, Via Belmeloro 6, 40126 Bologna, Italy
    Bioorg Med Chem 12:5525-32. 2004
    ..Unlike CoQ(0), which is considered a mitochondrial membrane permeability transition inhibitor, the new compounds were inducers...
  43. pmc Inefficient coupling between proton transport and ATP synthesis may be the pathogenic mechanism for NARP and Leigh syndrome resulting from the T8993G mutation in mtDNA
    Gianluca Sgarbi
    Dipartimento di Biochimica G Moruzzi, Via Irnerio 48, Universita di Bologna, 40126 Bologna, Italy
    Biochem J 395:493-500. 2006
    ..We discuss our findings in view of the current knowledge regarding the rotary mechanism of catalysis of the enzyme...
  44. ncbi request reprint Mitochondrial quinone reductases: complex I
    Giorgio Lenaz
    Dipartimento di Biochemica, Universita di Bologna, Italy
    Methods Enzymol 382:3-20. 2004
  45. ncbi request reprint Severe impairment of complex I-driven adenosine triphosphate synthesis in leber hereditary optic neuropathy cybrids
    Alessandra Baracca
    Dipartimento di Biochimica, University of Bologna, Italy
    Arch Neurol 62:730-6. 2005
    ..Leber hereditary optic neuropathy (LHON) is a maternally inherited form of central vision loss associated with mitochondrial DNA point mutations that affect the ND subunits of complex I...
  46. ncbi request reprint Structural organization of the mitochondrial respiratory chain
    Maria Luisa Genova
    Dipartimento di Biochimica G Moruzzi, Universita di Bologna, Bologna, Italy
    Ital J Biochem 52:58-61. 2003
    ....
  47. ncbi request reprint Yessotoxin, a shellfish biotoxin, is a potent inducer of the permeability transition in isolated mitochondria and intact cells
    Cristina Bianchi
    Department of Biochemistry G Moruzzi, University of Bologna, Via Irnerio 48, 40126 Bologna, Italy
    Biochim Biophys Acta 1656:139-47. 2004
    ....
  48. ncbi request reprint Rhodamine 123 as a probe of mitochondrial membrane potential: evaluation of proton flux through F(0) during ATP synthesis
    Alessandra Baracca
    Department of Biochemistry G Moruzzi Alma Mater Studiorum University of Bologna, Via Irnerio 48, I 40126, Bologna, Italy
    Biochim Biophys Acta 1606:137-46. 2003
    ....
  49. ncbi request reprint Hypoxia and mitochondrial oxidative metabolism
    Giancarlo Solaini
    Department of Biochemistry G Moruzzi, University of Bologna, Bologna, Italy
    Biochim Biophys Acta 1797:1171-7. 2010
    ..In addition, we address the involvement of HIF-1 and the inhibitor protein of F1F0 ATPase in the hypoxia-induced mitochondrial autophagy...
  50. ncbi request reprint Functional role of mitochondrial respiratory supercomplexes
    Maria Luisa Genova
    Dipartimento di Scienze Biomediche e Neuromotorie, Alma Mater Studiorum Universita di Bologna, Via Irnerio 48, 40126 Bologna, Italy Electronic address
    Biochim Biophys Acta 1837:427-43. 2014
    ..This article is part of a Special Issue entitled: Dynamic and ultrastructure of bioenergetic membranes and their components...
  51. doi request reprint Supramolecular organisation of the mitochondrial respiratory chain: a new challenge for the mechanism and control of oxidative phosphorylation
    Giorgio Lenaz
    Department of Biochemistry, University of Bologna, Bologna, Italy
    Adv Exp Med Biol 748:107-44. 2012
    ....
  52. ncbi request reprint Methods to detect mitochondrial function
    Milena Merlo-Pich
    Dipartimento di Biochimica G Moruzzi, University of Bologna, Via Irnerio 48, Bologna 40126, Italy
    Exp Gerontol 39:277-81. 2004
    ..Measurement of KCN-sensitive oxygen consumption by the cells and its sensitivity to uncouplers can be good supplementary indication of mitochondrial phosphorylative capacity...
  53. ncbi request reprint Impairment of mitochondrial oxidative phosphorylation in rat fatty liver exposed to preservation-reperfusion injury
    Paolo Caraceni
    Dipartimento di Biochimica, University of Bologna, 40138 Bologna, Italy
    J Hepatol 41:82-8. 2004
    ..As the impairment of the cellular energy metabolism contributes to the failure of fatty liver grafts after transplantation, we aimed to determine whether steatosis affects the oxidative phosphorylation activity during preservation...
  54. doi request reprint Mobility and function of coenzyme Q (ubiquinone) in the mitochondrial respiratory chain
    Giorgio Lenaz
    Dipartimento di Biochimica G Moruzzi, Universita di Bologna, Via Irnerio 48, 40126 Bologna, Italy
    Biochim Biophys Acta 1787:563-73. 2009
    ....
  55. ncbi request reprint Redox cycling of adrenaline and adrenochrome catalysed by mitochondrial Complex I
    Maria Luisa Genova
    Dipartimento di Biochimica G Moruzzi, Universita di Bologna, Via Irnerio 48, 40126 Bologna, Italy
    Arch Biochem Biophys 447:167-73. 2006
    ..The results are discussed in terms of current assays for superoxide detection and of pathologies linked to catecholamine oxidation...
  56. ncbi request reprint Novel class of quinone-bearing polyamines as multi-target-directed ligands to combat Alzheimer's disease
    Maria Laura Bolognesi
    Department of Pharmaceutical Sciences, Alma Mater Studiorum, University of Bologna, Via Belmeloro 6, Italy
    J Med Chem 50:4882-97. 2007
    ....
  57. doi request reprint A critical appraisal of the role of respiratory supercomplexes in mitochondria
    Maria Luisa Genova
    Department of Biomedical and Neuromotor Sciences, Laboratory of Mitochondrial Medicine, University of Bologna, Via Irnerio 48, I 40126 Bologna, Italy
    Biol Chem 394:631-9. 2013
    ..This review also discusses the implications concerning the number of respiratory complexes organized in supercomplexes and the possibility that they represent associations in dynamic equilibrium with the individual complexes...
  58. ncbi request reprint Evaluation of cellular energetics by the pasteur effect in intact cardiomyoblasts and isolated perfused hearts
    Claudio Muscari
    Department of Biochemistry G Moruzzi, University of Bologna, Bologna, Italy
    Mol Cell Biochem 258:91-7. 2004
    ..9%), it was 25% lower than control (p < 0.05). Therefore, we proposed this test as a tool for quantifying both physiological and pathological energetic modifications in living intact cardiomyocytes and in isolated and perfused hearts...
  59. ncbi request reprint Structural and functional organization of the mitochondrial respiratory chain: a dynamic super-assembly
    Giorgio Lenaz
    Dipartimento di Biochimica G Moruzzi, Universita di Bologna, Via Irnerio 48, 40126 Bologna, Italy
    Int J Biochem Cell Biol 41:1750-1772. 2009
    ..Finally, there is increasing evidence that disruption of the supercomplex organization leads to functional derangements responsible for pathological changes...
  60. doi request reprint Generation of reactive oxygen species by mitochondrial complex I: implications in neurodegeneration
    Romana Fato
    Dipartimento di Biochimica G Moruzzi, Universita di Bologna, Via Irnerio 48, 40126, Bologna, Italy
    Neurochem Res 33:2487-501. 2008
    ....
  61. ncbi request reprint Increased transcription of mitochondrial genes for Complex I in human platelets during ageing
    Milena Merlo Pich
    Department of Biochemistry G Moruzzi, University of Bologna, Via Irnerio 48, 40126 Bologna, Italy
    FEBS Lett 558:19-22. 2004
    ..A significant increase of transcripts for Complex I was found during ageing. On the contrary, the mRNA levels of the two COX subunits did not significantly vary during ageing...
  62. ncbi request reprint The site of production of superoxide radical in mitochondrial Complex I is not a bound ubisemiquinone but presumably iron-sulfur cluster N2
    M L Genova
    Dipartimento di Biochimica G Moruzzi, Universita di Bologna, Via Irnerio 48, 40126, Bologna, Italy
    FEBS Lett 505:364-8. 2001
    ..Short chain CoQ analogs enhance superoxide formation, presumably by mediating electron transfer from N2 to oxygen. The clinically used CoQ analog, idebenone, is particularly effective in promoting superoxide formation...
  63. doi request reprint Oxidative stress in the denervated muscle
    Provvidenza M Abruzzo
    Department of Histology, Embryology, and Applied Biology, University of Bologna, Italy
    Free Radic Res 44:563-76. 2010
    ....
  64. ncbi request reprint Evaluating mitochondrial membrane potential in cells
    Giancarlo Solaini
    Dipartimento di Biochimica, Universita di Bologna, Via Irnerio 48, Bologna, 40126, Italy
    Biosci Rep 27:11-21. 2007
    ..Furthermore, we discuss the application and feasibility of various techniques and discuss their respective strength and weakness...
  65. ncbi request reprint Is supercomplex organization of the respiratory chain required for optimal electron transfer activity?
    M L Genova
    Dipartimento di Biochimica G Moruzzi, Alma Mater Studiorum Universita di Bologna, Via Irnerio 48, 40126 Bologna, Italy
    Biochim Biophys Acta 1777:740-6. 2008
    ....
  66. pmc Naphthoquinone derivatives exert their antitrypanosomal activity via a multi-target mechanism
    Simone Pieretti
    Department of Pharmacy and Biotechnology, University of Bologna, Bologna, Italy
    PLoS Negl Trop Dis 7:e2012. 2013
    ..To disclose such a multitarget profile of B6, we exploited a chemical proteomics approach...
  67. ncbi request reprint Biochemical-clinical correlation in patients with different loads of the mitochondrial DNA T8993G mutation
    Valerio Carelli
    Istituto di Clinica Neurologica, Universita di Bologna, Via U Foscolo 7, 40123 Bologna, Italy
    Arch Neurol 59:264-70. 2002
    ....
  68. ncbi request reprint Apoptosis-resistant phenotype in HL-60-derived cells HCW-2 is related to changes in expression of stress-induced proteins that impact on redox status and mitochondrial metabolism
    S Salvioli
    Department of Experimental Pathology, University of Bologna, Bologna, Italy
    Cell Death Differ 10:163-74. 2003
    ..These observations could have some implications for the therapy of cancer, and for the design of combined strategies that act on antioxidant defences of the neoplastic cell...
  69. ncbi request reprint Functional alterations of the mitochondrially encoded ND4 subunit associated with Leber's hereditary optic neuropathy
    M Degli Esposti
    Department of Biology, Institute of Clinical Neurology, University of Bologna, Italy
    FEBS Lett 352:375-9. 1994
    ..Such changes could reflect a substantial loss in the energy conserving function of NADH:ubiquinone reductase and thus explain the pathological effect of the ND4/11,778 mutation...
  70. ncbi request reprint Effect of micronutrient status on natural killer cell immune function in healthy free-living subjects aged >/=90 y
    G Ravaglia
    Department of Internal Medicine, Cardioangiology, and Hepatology, the Department of Angiology and Blood Coagulation, and the Division of Geriatric Medicine, University Hospital Sant Orsola Malpighi, Bologna, Italy
    Am J Clin Nutr 71:590-8. 2000
    ..Natural killer (NK) cells play a role in natural immunity against tumor and infected cells. Advanced aging is associated with functional impairment of NK cells and increased susceptibility to nutritional deficiencies...
  71. doi request reprint Mitochondria and reactive oxygen species. Which role in physiology and pathology?
    Giorgio Lenaz
    Dipartimento di Biochimica, Universita di Bologna, Bologna, Italy
    Adv Exp Med Biol 942:93-136. 2012
    ..Oxidative stress is at the basis of ageing and many pathological disorders, such as ischemic diseases, neurodegenerative diseases, diabetes, and cancer, although the underlying mechanisms are not always completely understood...
  72. pmc Hydrophobic alpha-helices 1 and 2 of herpes simplex virus gH interact with lipids, and their mimetic peptides enhance virus infection and fusion
    Tatiana Gianni
    Department of Experimental Pathology, Section on Microbiology and Virology, University of Bologna, Via San Giacomo, 12 40126 Bologna, Italy
    J Virol 80:8190-8. 2006
    ..However, alpha-H2 differs from alpha-H1 in that it is of lower hydrophobicity and cannot be replaced with heterologous fusion peptides...
  73. ncbi request reprint Changes in mitochondrial complex I activity and coenzyme Q binding site in Leber's hereditary optic neuropathy (LHON)
    A Ghelli
    Department of Biochemistry G Moruzzi, University of Bologna, Italy
    Mol Aspects Med 18:S263-7. 1997
    ..It was concluded that even with different pathogenic mechanisms both mutations affect the quinone binding site of complex I...
  74. pmc Fluorescence resonance energy transfer between coumarin-derived mitochondrial F(1)-ATPase gamma subunit and pyrenylmaleimide-labelled fragments of IF(1) and c subunit
    Alessandra Baracca
    Dipartimento di Biochimica G Moruzzi, Universita degli Studi di Bologna, Via Irnerio 48, 40126 Bologna, Italy
    Biochem J 362:165-71. 2002
    ....
  75. ncbi request reprint Decreased Pasteur effect in platelets of aged individuals
    M Merlo Pich
    Dipartimento di Biochimica G. Moruzzi, University of Bologna, Via Irnerio 48, 40126 Bologna, Italy
    Mech Ageing Dev 122:823-33. 2001
    ....
  76. ncbi request reprint Mitochondrial changes induced by natural and synthetic asbestos fibers: studies on isolated mitochondria
    C Bergamini
    Department of Biochemistry G Moruzzi, University of Bologna, Via Irnerio 48, 40126 Bologna, Italy
    Cell Mol Biol (Noisy-le-grand) 52:OL905-13. 2006
    ..Nevertheless, all asbestos fibers tested induce morphological alterations visualized by transmission electron microscopy and morphometric analysis...
  77. ncbi request reprint Biochemical features of mtDNA 14484 (ND6/M64V) point mutation associated with Leber's hereditary optic neuropathy
    V Carelli
    Istituto di Clinica Neurologica, , Italy
    Ann Neurol 45:320-8. 1999
    ....
  78. ncbi request reprint Interactions and effects of 2-hydroxy-5-nitrobenzyl bromide on the bovine heart mitochondrial F1-ATPase
    A Baracca
    Department of Biochemistry Giovanni Moruzzi, University of Bologna, Italy
    Int J Biochem 25:1269-75. 1993
    ..5. The possibility that the epsilon-subunit of mitochondrial F1-ATPase may influence the functional or regulating domain of the enzyme is discussed...
  79. ncbi request reprint Mitochondrial changes induced by natural and synthetic asbestos fibers: studies on isolated mitochondria
    C Bergamini
    Department of Biochemistry G Moruzzi, University of Bologna, 40126 Bologna, Italy
    Cell Mol Biol (Noisy-le-grand) 50:OL691-700. 2004
    ..Nevertheless, all asbestos fibers tested induce morphological alterations visualized by transmission electron microscopy and morphometric analysis...
  80. ncbi request reprint Isolation and subfractionation of mitochondria from animal cells and tissue culture lines
    Francesco Pallotti
    Dipartimento di Scienze Biomediche Sperimentali e Cliniche, Universita degli Studi dell Insubria, 21100 Varese, Italy
    Methods Cell Biol 80:3-44. 2007
  81. ncbi request reprint Respiratory chain supercomplexes set the threshold for respiration defects in human mtDNA mutant cybrids
    Marilena D'Aurelio
    Department of Neurology and Neuroscience, Weill Medical College of Cornell University, New York, NY 10021, USA
    Hum Mol Genet 15:2157-69. 2006
    ..From these findings, supercomplex assembly emerges as a necessary step for respiration, and its defect sets the threshold for respiratory impairment in mtDNA mutant cells...
  82. ncbi request reprint Defective oxidative phosphorylation in thyroid oncocytic carcinoma is associated with pathogenic mitochondrial DNA mutations affecting complexes I and III
    Elena Bonora
    Unità di Genetica Medica, Policlinico Universitario S Orsola Malpighi, Italy
    Cancer Res 66:6087-96. 2006
    ..UC1 is due to a combined complex I/III defect associated with mtDNA mutations, as proven by the transfer of the defective energetic phenotype with the mitochondrial genome into the cybrids...
  83. ncbi request reprint Glycerophosphate-dependent hydrogen peroxide production by brown adipose tissue mitochondria and its activation by ferricyanide
    Zdenek Drahota
    Institute of Physiology and Center for Integrated Genomics, Academy of Sciences of the Czech Republic, Prague
    J Bioenerg Biomembr 34:105-13. 2002
    ..The data suggest that more intensive production of reactive oxygen species may be present in mammalian cells with active mGPDH...
  84. ncbi request reprint Coenzyme Q releases the inhibitory effect of free fatty acids on mitochondrial glycerophosphate dehydrogenase
    Hana Rauchová
    Center for Experimental Cardiovascular Research, Czech Academy of Sciences, 142 20 Prague 4, Videnska 1083, Czech Republic
    Acta Biochim Pol 50:405-13. 2003
    ..The presented data suggest differences between mitochondrial glycerophosphate dehydrogenase and succinate or NADH dehydrogenases in the transfer of reducing equivalents to the CoQ pool...
  85. ncbi request reprint The mtDNA T8993G (NARP) mutation results in an impairment of oxidative phosphorylation that can be improved by antioxidants
    Marina Mattiazzi
    Department of Neurology and Neuroscience, Weill Medical College of Cornell University, New York, USA
    Hum Mol Genet 13:869-79. 2004
    ..The effectiveness of antioxidant agents in cultured NARP/MILS cells suggests that they might have a potential beneficial role in the treatment of patients with NARP...
  86. pmc Biochemical analysis of respiratory function in cybrid cell lines harbouring mitochondrial DNA mutations
    Francesco Pallotti
    Department of Neurology, College of Physicians and Surgeons, Columbia University, 630 West 168th Street, New York, NY 10032, USA
    Biochem J 384:287-93. 2004
    ..However, the distinct clinical features associated with some of these mutations still remain to be elucidated...
  87. ncbi request reprint Inhibition of glycerophosphate-dependent H2O2 generation in brown fat mitochondria by idebenone
    Hana Rauchová
    Institute of Physiology, Academy of Sciences of the Czech Republic, Czech Republic
    Biochem Biophys Res Commun 339:362-6. 2006
    ..8 microM). Idebenone also inhibited ferricyanide (FeCN)-activated, GP-dependent ROS production. Our data thus extend previous findings on the scavenging effect of idebenone and show that it can also eliminate GP-dependent ROS generation...
  88. ncbi request reprint Heterologous mitochondrial DNA recombination in human cells
    Marilena D'Aurelio
    Department of Neurology and Neuroscience, Weill Medical College of Cornell University, New York, NY, USA
    Hum Mol Genet 13:3171-9. 2004
    ..These findings have important implications for mtDNA-related diseases, the interpretation of human evolution and population genetics and forensic analyses based on mtDNA genotyping...