Research Topics
Species | Giorgio LenazSummaryAffiliation: University of Bologna Country: Italy Publications
| Collaborators
|
Detail Information
Publications
Mitochondria and reactive oxygen species. Which role in physiology and pathology?Giorgio Lenaz
Dipartimento di Biochimica, Universita di Bologna, Bologna, Italy
Adv Exp Med Biol 942:93-136. 2012..Oxidative stress is at the basis of ageing and many pathological disorders, such as ischemic diseases, neurodegenerative diseases, diabetes, and cancer, although the underlying mechanisms are not always completely understood...- Mobility and function of coenzyme Q (ubiquinone) in the mitochondrial respiratory chainGiorgio Lenaz
Dipartimento di Biochimica G Moruzzi, Universita di Bologna, Via Irnerio 48, 40126 Bologna, Italy
Biochim Biophys Acta 1787:563-73. 2009.... 
Structural and functional organization of the mitochondrial respiratory chain: a dynamic super-assemblyGiorgio Lenaz
Dipartimento di Biochimica G Moruzzi, Universita di Bologna, Via Irnerio 48, 40126 Bologna, Italy
Int J Biochem Cell Biol 41:1750-1772. 2009..Finally, there is increasing evidence that disruption of the supercomplex organization leads to functional derangements responsible for pathological changes...- Structure and organization of mitochondrial respiratory complexes: a new understanding of an old subjectGiorgio Lenaz
Dipartimento di Biochimica G Moruzzi, Alma Mater Studiorum, Universita di Bologna, Bologna, Italy
Antioxid Redox Signal 12:961-1008. 2010..Disruption of supercomplex organization may lead to functional derangements responsible for pathologic changes... - The mitochondrial production of reactive oxygen species in relation to aging and pathologyMaria Luisa Genova
Dipartimento di Biochimica G. Moruzzi, University of Bologna, 40126 Bologna, Italy
Ann N Y Acad Sci 1011:86-100. 2004.... - Polyamine conjugation of curcumin analogues toward the discovery of mitochondria-directed neuroprotective agentsElena Simoni
Department of Pharmaceutical Sciences, University of Bologna, Via Belmeloro 6, 40126 Bologna, Italy
J Med Chem 53:7264-8. 2010..It also resulted in a significant decrease in the cytotoxicity effects. 2-5 are therefore promising molecules for neuroprotectant lead discovery... - Toward a rational design of multitarget-directed antioxidants: merging memoquin and lipoic acid molecular frameworksMaria Laura Bolognesi
Department of Pharmaceutical Sciences, University of Bologna, Via Belmeloro 6, 40126 Bologna, Italy
J Med Chem 52:7883-6. 2009..They also displayed a balanced inhibitory profile against amyloid-beta aggregation and acetylcholinesterase, emerging as promising molecules for neuroprotectant lead discovery... - The mitochondrial respiratory chain is partially organized in a supercomplex assembly: kinetic evidence using flux control analysisCristina Bianchi
Dipartimento di Biochimica G Moruzzi, Universita di Bologna, Via Irnerio 48, 40126 Bologna, Italy
J Biol Chem 279:36562-9. 2004..Moreover, we show that Complex II is fully rate-limiting for succinate oxidation, clearly indicating the absence of substrate channeling toward Complexes III and IV... - Mitochondrial respiratory chain super-complex I-III in physiology and pathologyGiorgio Lenaz
Dipartimento di Biochimica, Universita di Bologna, Via Irnerio 48, 40126 Bologna, Italy
Biochim Biophys Acta 1797:633-40. 2010..There is increasing evidence that disruption of the super-complex organization leads to functional derangements responsible for pathological changes, as we have found in K-ras-transformed fibroblasts... - Biochemical phenotypes associated with the mitochondrial ATP6 gene mutations at nt8993Alessandra Baracca
Dipartimento di Biochimica G Moruzzi, Via Irnerio 48, Universita di Bologna, 40126 Bologna, and Fondazione Ospedale Maggiore IRCCS Centro Dino Ferrari, Milano, Italy
Biochim Biophys Acta 1767:913-9. 2007.... - Potential antitumor agents. 37. Synthesis and antitumor activity of guanylhydrazones from imidazo[2,1-b]thiazoles and from the new heterocyclic system thiazolo[2',3':2,3]imidazo[4,5-c]quinolineAldo Andreani
Dipartimento di Scienze Farmaceutiche, Universita di Bologna, Via Belmeloro 6, 40126 Bologna, Italy
J Med Chem 48:3085-9. 2005.... - Mitochondrial activities of a cell line derived from thyroid Hürthle cell tumorsKarmen Stankov
Dipartimento di Medicina Interna, Cardioangiologia ed Epatologia, Unita' Operativa di Genetica Medica, Policlinico S. Orsola-Malpighi, Bologna, Italy
Thyroid 16:325-31. 2006.... - Mitochondrial Complex I decrease is responsible for bioenergetic dysfunction in K-ras transformed cellsAlessandra Baracca
Department of Biochemistry G Moruzzi, University of Bologna, Bologna, Italy
Biochim Biophys Acta 1797:314-23. 2010..Taken together, our results provide the new insight that the reduction of respiration observed in K-ras transformed cells is specifically due to a Complex I activity decrease... - Ischemic preconditioning preserves proton leakage from mitochondrial membranes but not oxidative phosphorylation during heart reperfusionClaudio Muscari
Department of Biochemistry G Moruzzi, University of Bologna, Via Irnerio 48, 40126 Bologna, Italy
Cell Biochem Funct 24:511-8. 2006..Mitochondria can contribute to cell survival by the attenuation of proton leak from inner membrane... - Plasma membrane oxidoreductase activity in cultured cells in relation to mitochondrial function and oxidative stressGiulia Deleonardi
Dipartimento di Biochimica, , Via Irnerio 48, 40126 Bologna, Italy
Biofactors 20:251-8. 2004..The results suggest that enhanced oxidative stress and not deficient respiratory activity per se is the stimulus triggering over-expression of plasma membrane oxidative enzymes... 
Differential effects of mitochondrial Complex I inhibitors on production of reactive oxygen speciesRomana Fato
Dipartimento di Biochimica G Moruzzi, University of Bologna, Via Irnerio 48, 40126 Bologna, Italy
Biochim Biophys Acta 1787:384-92. 2009..The results suggest that electron transfer from the iron-sulphur clusters (N2) to Coenzyme Q occurs in two steps gated by two different conformations, the former being sensitive to Rotenone and the latter to Stigmatellin...- New insights into structure and function of mitochondria and their role in aging and diseaseGiorgio Lenaz
Dipartimento di Biochimica, Universita di Bologna, Bologna, Italy
Antioxid Redox Signal 8:417-37. 2006..Evidence is presented favoring the mitochondrial theory, with primary mitochondrial alterations, although the problem is made more complex by changes in the cross-talk between nuclear and mitochondrial DNA... - Supercomplex organization of the mitochondrial respiratory chain and the role of the Coenzyme Q pool: pathophysiological implicationsMaria Luisa Genova
Dipartimento di Biochimica G Moruzzi, Universita di Bologna, Italy
Biofactors 25:5-20. 2005..This finding has pathophysiological implications, in view of the role of oxidative stress in the pathogenesis of many diseases... - Human NARP mitochondrial mutation metabolism corrected with alpha-ketoglutarate/aspartate: a potential new therapyGianluca Sgarbi
Dipartimento di Biochimica G Moruzzi, Universita di Bologna, Bologna, Italy
Arch Neurol 66:951-7. 2009.... - Bioenergetics of mitochondrial diseases associated with mtDNA mutationsGiorgio Lenaz
Dipartimento di Biochimica G. Moruzzi, , Via Irnerio 48, Bologna 40126, Italy
Biochim Biophys Acta 1658:89-94. 2004.... - A water soluble CoQ10 formulation improves intracellular distribution and promotes mitochondrial respiration in cultured cellsChristian Bergamini
Department of Biochemistry G Moruzzi, University of Bologna, Bologna, Italy
PLoS ONE 7:e33712. 2012..Controversial clinical and in vitro results are mainly due to the high hydrophobicity of this compound, which reduces its bioavailability... - The study of the pathogenic mechanism of mitochondrial diseases provides information on basic bioenergeticsGiancarlo Solaini
Dipartimento di Biochimica G Moruzzi, Via Irnerio, 48, Universita di Bologna, Italy
Biochim Biophys Acta 1777:941-5. 2008..We propose that the integrity of transmembrane helix III is essential for the mechanical function of ATPase 6 as a stator element in the ATP synthase, but that it is not relevant for oligomycin inhibition... - The role of Coenzyme Q in mitochondrial electron transportGiorgio Lenaz
Dipartimento di Biochimica, Universita di Bologna, Via Irnerio 48, 40126 Bologna, Italy
Mitochondrion 7:S8-33. 2007..metabolic channeling, respectively, both in physiological and in some pathological conditions. As an example of intra-complex electron transfer, we discuss in particular Complex I, a topic that is still under active investigation... - Mitochondrial production of oxygen radical species and the role of Coenzyme Q as an antioxidantMaria Luisa Genova
Dipartimento di Biochimica G Moruzzi, University of Bologna, 40126 Bologna, Italy
Exp Biol Med (Maywood) 228:506-13. 2003..The plasma membrane oxidoreductase and DT-diaphorase are two such systems, likewise, they are overexpressed under oxidative stress conditions... - Mitochondrial production of reactive oxygen species: role of complex I and quinone analoguesRomana Fato
Dipartimento di Biochimica G Moruzzi, University of Bologna, 40126 Bologna, Italy
Biofactors 32:31-9. 2008.... - Structural and functional organization of Complex I in the mitochondrial respiratory chainCristina Bianchi
Universtiy of Bologna, Bologna, Italy
Biofactors 18:3-9. 2003.... - Ubiquinol and a coenzyme Q reducing system protect platelet mitochondrial function of transfusional buffy coats from oxidative stressMilena Merlo Pich
Dipartimento di Biochimica G. Moruzzi, , Italy
Free Radic Res 36:429-36. 2002.... - Severe impairment of complex I-driven adenosine triphosphate synthesis in leber hereditary optic neuropathy cybridsAlessandra Baracca
Dipartimento di Biochimica, University of Bologna, Italy
Arch Neurol 62:730-6. 2005..Stratification of the results by mutation suggests that the 11778/ND4 mutation may induce an uncoupling of cybrid respiration, whereas the other 2 mutations impair the oxygen consumption rate... - New developments on the functions of coenzyme Q in mitochondriaMaria Luisa Genova
Dipartimento di Biochimica G Moruzzi, Universita di Bologna, Italy
Biofactors 37:330-54. 2011..Finally, a working hypothesis is advanced on the establishment of a vicious circle of oxidative stress and supercomplex disorganization in pathological states, as in neurodegeneration and cancer... - Inefficient coupling between proton transport and ATP synthesis may be the pathogenic mechanism for NARP and Leigh syndrome resulting from the T8993G mutation in mtDNAGianluca Sgarbi
Dipartimento di Biochimica G. Moruzzi, Via Irnerio 48, , 40126 Bologna, Italy
Biochem J 395:493-500. 2006..We discuss our findings in view of the current knowledge regarding the rotary mechanism of catalysis of the enzyme... - Bioenergetics shapes cellular death pathways in Leber's hereditary optic neuropathy: a model of mitochondrial neurodegenerationValerio Carelli
Dipartimento di Scienze Neurologiche, Universita di Bologna, Via Ugo Foscolo 7, 40123 Bologna, Italy
Biochim Biophys Acta 1658:172-9. 2004.... - Mitochondrial Complex I: structure, function, and implications in neurodegenerationGiorgio Lenaz
Dipartimento di Biochimica G Moruzzi, Universita di Bologna, Italy
Ital J Biochem 55:232-53. 2006.... - Mitochondrial Complex I: structural and functional aspectsGiorgio Lenaz
Department of Biochemistry, University of Bologna, Via Irnerio 48, 40126 Bologna, Italy
Biochim Biophys Acta 1757:1406-20. 2006..The implications in human pathology of better knowledge on these aspects of Complex I structure and function are briefly discussed... - Yessotoxin, a shellfish biotoxin, is a potent inducer of the permeability transition in isolated mitochondria and intact cellsCristina Bianchi
Department of Biochemistry G. Moruzzi, University of Bologna, Via Irnerio 48, 40126 Bologna, Italy
Biochim Biophys Acta 1656:139-47. 2004.... - Kinetics of integrated electron transfer in the mitochondrial respiratory chain: random collisions vs. solid state electron channelingGiorgio Lenaz
Dipartimento di Biochimica G Moruzzi, Via Irnerio 48, 40126 Bologna, Italy
Am J Physiol Cell Physiol 292:C1221-39. 2007.... - Hypoxia and mitochondrial oxidative metabolismGiancarlo Solaini
Department of Biochemistry G Moruzzi, University of Bologna, Bologna, Italy
Biochim Biophys Acta 1797:1171-7. 2010..In addition, we address the involvement of HIF-1 and the inhibitor protein of F1F0 ATPase in the hypoxia-induced mitochondrial autophagy... - Control of oxidative phosphorylation by Complex I in rat liver mitochondria: implications for agingBarbara Ventura
Dipartimento di Biochimica G. Moruzzi, , Via Irnerio 48, 40126, Bologna, Italy
Biochim Biophys Acta 1553:249-60. 2002..Our results point out that age-related alterations of the mitochondrial functions are also present in tissues considered less prone to accumulate mitochondrial DNA mutations... - Mitochondrial quinone reductases: complex IGiorgio Lenaz
Dipartimento di Biochemica, Universita di Bologna, Italy
Methods Enzymol 382:3-20. 2004 - Supramolecular organisation of the mitochondrial respiratory chain: a new challenge for the mechanism and control of oxidative phosphorylationGiorgio Lenaz
Department of Biochemistry, University of Bologna, Bologna, Italy
Adv Exp Med Biol 748:107-44. 2012.... - Effects of new ubiquinone-imidazo[2,1-b]thiazoles on mitochondrial complex I (NADH-ubiquinone reductase) and on mitochondrial permeability transition poreAldo Andreani
Dipartimento di Scienze Farmaceutiche, Universita di Bologna, Via Belmeloro 6, 40126 Bologna, Italy
Bioorg Med Chem 12:5525-32. 2004..Unlike CoQ(0), which is considered a mitochondrial membrane permeability transition inhibitor, the new compounds were inducers... - Structural organization of the mitochondrial respiratory chainMaria Luisa Genova
Dipartimento di Biochimica G Moruzzi, Universita di Bologna, Bologna, Italy
Ital J Biochem 52:58-61. 2003.... - Role of mitochondria in oxidative stress and agingGiorgio Lenaz
Dipartimento di Biochimica G Moruzzi, Universita di Bologna, Via Irnerio 48, 40126 Bologna, Italy
Ann N Y Acad Sci 959:199-213. 2002..The plasma membrane oxidoreductase and DT-diaphorase are two such systems: likewise, they are overexpressed under oxidative stress conditions... - Impairment of mitochondrial oxidative phosphorylation in rat fatty liver exposed to preservation-reperfusion injuryPaolo Caraceni
Dipartimento di Biochimica, University of Bologna, 40138 Bologna, Italy
J Hepatol 41:82-8. 2004..As the impairment of the cellular energy metabolism contributes to the failure of fatty liver grafts after transplantation, we aimed to determine whether steatosis affects the oxidative phosphorylation activity during preservation... - Redox cycling of adrenaline and adrenochrome catalysed by mitochondrial Complex IMaria Luisa Genova
Dipartimento di Biochimica G Moruzzi, Universita di Bologna, Via Irnerio 48, 40126 Bologna, Italy
Arch Biochem Biophys 447:167-73. 2006..The results are discussed in terms of current assays for superoxide detection and of pathologies linked to catecholamine oxidation... - Rhodamine 123 as a probe of mitochondrial membrane potential: evaluation of proton flux through F(0) during ATP synthesisAlessandra Baracca
Department of Biochemistry G Moruzzi Alma Mater Studiorum University of Bologna, Via Irnerio 48, I 40126, Bologna, Italy
Biochim Biophys Acta 1606:137-46. 2003.... - Novel class of quinone-bearing polyamines as multi-target-directed ligands to combat Alzheimer's diseaseMaria Laura Bolognesi
Department of Pharmaceutical Sciences, Alma Mater Studiorum, University of Bologna, Via Belmeloro 6, Italy
J Med Chem 50:4882-97. 2007.... - Methods to detect mitochondrial functionMilena Merlo-Pich
Dipartimento di Biochimica G Moruzzi, University of Bologna, Via Irnerio 48, Bologna 40126, Italy
Exp Gerontol 39:277-81. 2004..Measurement of KCN-sensitive oxygen consumption by the cells and its sensitivity to uncouplers can be good supplementary indication of mitochondrial phosphorylative capacity... - Increased transcription of mitochondrial genes for Complex I in human platelets during ageingMilena Merlo Pich
Department of Biochemistry G Moruzzi, University of Bologna, Via Irnerio 48, 40126 Bologna, Italy
FEBS Lett 558:19-22. 2004..A significant increase of transcripts for Complex I was found during ageing. On the contrary, the mRNA levels of the two COX subunits did not significantly vary during ageing... - Generation of reactive oxygen species by mitochondrial complex I: implications in neurodegenerationRomana Fato
Dipartimento di Biochimica G Moruzzi, Universita di Bologna, Via Irnerio 48, 40126, Bologna, Italy
Neurochem Res 33:2487-501. 2008.... - Evaluation of cellular energetics by the pasteur effect in intact cardiomyoblasts and isolated perfused heartsClaudio Muscari
Department of Biochemistry G Moruzzi, University of Bologna, Bologna, Italy
Mol Cell Biochem 258:91-7. 2004..9%), it was 25% lower than control (p < 0.05). Therefore, we proposed this test as a tool for quantifying both physiological and pathological energetic modifications in living intact cardiomyocytes and in isolated and perfused hearts... - Evaluating mitochondrial membrane potential in cellsGiancarlo Solaini
Dipartimento di Biochimica, Universita di Bologna, Via Irnerio 48, Bologna, 40126, Italy
Biosci Rep 27:11-21. 2007..Furthermore, we discuss the application and feasibility of various techniques and discuss their respective strength and weakness... - Enhanced activity of the plasma membrane oxidoreductase in circulating lymphocytes from insulin-dependent diabetes mellitus patientsGiorgio Lenaz
Dipartimento di Biochimica, Universita di Bologna, Via Irnerio 48, Bologna 40126, Italy
Biochem Biophys Res Commun 290:1589-92. 2002..The increase of PMOR in diabetes is likely due to overexpression of the enzyme, in view of the very low K(m) for NADH indicating that, in intact cells, the enzyme is practically saturated with the reductant substrate... - Biochemical-clinical correlation in patients with different loads of the mitochondrial DNA T8993G mutationValerio Carelli
Istituto di Clinica Neurologica, Universita di Bologna, Via U Foscolo 7, 40123 Bologna, Italy
Arch Neurol 59:264-70. 2002.... - Fluorescence resonance energy transfer between coumarin-derived mitochondrial F(1)-ATPase gamma subunit and pyrenylmaleimide-labelled fragments of IF(1) and c subunitAlessandra Baracca
Dipartimento di Biochimica G. Moruzzi, , Via Irnerio 48, 40126 Bologna, Italy
Biochem J 362:165-71. 2002....