K Helin

Summary

Affiliation: University of Milan
Country: Italy

Publications

  1. ncbi Regulation of cell proliferation by the E2F transcription factors
    K Helin
    European Institute of Oncology, Department of Experimental Oncology, Milan, Italy
    Curr Opin Genet Dev 8:28-35. 1998
  2. ncbi Loss of the retinoblastoma protein-related p130 protein in small cell lung carcinoma
    K Helin
    Department of Experimental Oncology, European Institute of Oncology, 20141 Milan, Italy
    Proc Natl Acad Sci U S A 94:6933-8. 1997
  3. ncbi CDC25A phosphatase is a target of E2F and is required for efficient E2F-induced S phase
    E Vigo
    Department of Experimental Oncology, European Institute of Oncology, 20141 Milan, Italy
    Mol Cell Biol 19:6379-95. 1999
  4. ncbi Mad2 binding to Mad1 and Cdc20, rather than oligomerization, is required for the spindle checkpoint
    L Sironi
    Department of Experimental Oncology, European Institute of Oncology, Via Ripamonti 435, 20141 Milan, Italy
    EMBO J 20:6371-82. 2001
  5. ncbi Cell cycle- and cell growth-regulated proteolysis of mammalian CDC6 is dependent on APC-CDH1
    B O Petersen
    Department of Experimental Oncology, European Institute of Oncology, 20141 Milan, Italy
    Genes Dev 14:2330-43. 2000
  6. ncbi Apaf-1 is a transcriptional target for E2F and p53
    M C Moroni
    Department of Experimental Oncology, European Institute of Oncology, Via Ripamonti 435, 20141 Milan, Italy
    Nat Cell Biol 3:552-8. 2001
  7. ncbi Cell cycle-regulated expression of mammalian CDC6 is dependent on E2F
    G Hateboer
    Department of Experimental Oncology, European Institute of Oncology, 20141 Milan, Italy
    Mol Cell Biol 18:6679-97. 1998
  8. ncbi E2F-6: a novel member of the E2F family is an inhibitor of E2F-dependent transcription
    P Cartwright
    European Institute of Oncology, Department of Experimental Oncology, Milan, Italy
    Oncogene 17:611-23. 1998
  9. ncbi The E2F transcription factors: key regulators of cell proliferation
    H Muller
    European Institute of Oncology, Department of Experimental Oncology, Via Ripamonti 435, 20141, Milan, Italy
    Biochim Biophys Acta 1470:M1-12. 2000
  10. ncbi Phosphorylation of mammalian CDC6 by cyclin A/CDK2 regulates its subcellular localization
    B O Petersen
    European Institute of Oncology, Department of Experimental Oncology, Via Ripamonti 435, 20141 Milan, Italy
    EMBO J 18:396-410. 1999

Collaborators

  • H Muller
  • J M Peters
  • M Alcalay
  • H Eiberg
  • F Cecconi
  • A Musacchio
  • Nicholas Dyson
  • Jiri Bartek
  • M Vidal
  • Brian D Dynlacht
  • Minoru Saito
  • H T Orr
  • B O Petersen
  • M C Moroni
  • E S Hickman
  • Z Lu
  • E Vigo
  • P Cartwright
  • G Hateboer
  • J Lukas
  • L Sironi
  • M Koziczak
  • E Lazzerini Denchi
  • M Melixetian
  • E Prosperini
  • C Wagener
  • W S L Liao
  • R Z Luo
  • A Nishmoto
  • M Huang
  • C Holmberg
  • H Peng
  • W S-L Liao
  • Y Yu
  • K K Hunt
  • P J Skinner
  • E Colli
  • Y Nagamine
  • G Caprara
  • M Faretta
  • F Marinoni
  • C Matteucci
  • E R Kramer
  • C Gieffers
  • E Soravia
  • C S Sørensen
  • E Harlow
  • O Karlstrom
  • M Sehested
  • A Wobst
  • L Le Cam
  • C Sardet
  • K Holm
  • S I Reed
  • K Hansen
  • T Herzinger
  • I A Klement
  • A Servadio
  • B T Koshy
  • D Resnitzky
  • C J Cummings
  • H Y Zoghbi
  • J A Lees
  • A R Fattaey
  • L Zhu
  • J L Degen
  • A Grebe
  • F Blasi
  • T T Suh
  • P De Luca
  • S van den Heuvel
  • A Fattaey
  • D Livingston
  • T Look
  • D DeFeo-Jones
  • G A Vuocolo
  • M Ewen
  • M S Dembski
  • K M Haskell
  • M Valentine
  • A Oliff
  • M G Hanobik

Detail Information

Publications26

  1. ncbi Regulation of cell proliferation by the E2F transcription factors
    K Helin
    European Institute of Oncology, Department of Experimental Oncology, Milan, Italy
    Curr Opin Genet Dev 8:28-35. 1998
    ..Novel target genes for the E2F transcription factors have been identified that link the E2Fs directly to the initiation of DNA replication...
  2. ncbi Loss of the retinoblastoma protein-related p130 protein in small cell lung carcinoma
    K Helin
    Department of Experimental Oncology, European Institute of Oncology, 20141 Milan, Italy
    Proc Natl Acad Sci U S A 94:6933-8. 1997
    ....
  3. ncbi CDC25A phosphatase is a target of E2F and is required for efficient E2F-induced S phase
    E Vigo
    Department of Experimental Oncology, European Institute of Oncology, 20141 Milan, Italy
    Mol Cell Biol 19:6379-95. 1999
    ..Taken together, our results provide an important step in defining how E2F activity leads to deregulated proliferation...
  4. ncbi Mad2 binding to Mad1 and Cdc20, rather than oligomerization, is required for the spindle checkpoint
    L Sironi
    Department of Experimental Oncology, European Institute of Oncology, Via Ripamonti 435, 20141 Milan, Italy
    EMBO J 20:6371-82. 2001
    ..We propose a model that features the kinetochore as a 'folding factory' for the formation of a Mad2-Cdc20 complex endowed with inhibitory activity on the anaphase promoting complex...
  5. ncbi Cell cycle- and cell growth-regulated proteolysis of mammalian CDC6 is dependent on APC-CDH1
    B O Petersen
    Department of Experimental Oncology, European Institute of Oncology, 20141 Milan, Italy
    Genes Dev 14:2330-43. 2000
    ..The APC-CDH1-dependent proteolysis of CDC6 in early G(1) and in quiescent cells suggests that this process is part of a mechanism that ensures the timely licensing of replication origins during G(1)...
  6. ncbi Apaf-1 is a transcriptional target for E2F and p53
    M C Moroni
    Department of Experimental Oncology, European Institute of Oncology, Via Ripamonti 435, 20141 Milan, Italy
    Nat Cell Biol 3:552-8. 2001
    ..We also show that, independently of the pRB pathway, Apaf-1 is a direct transcriptional target of p53, suggesting that p53 might sensitize cells to apoptosis by increasing Apaf-1 levels...
  7. ncbi Cell cycle-regulated expression of mammalian CDC6 is dependent on E2F
    G Hateboer
    Department of Experimental Oncology, European Institute of Oncology, 20141 Milan, Italy
    Mol Cell Biol 18:6679-97. 1998
    ..In conclusion, our results provide a direct link between regulated progression through G1 controlled by the pRB pathway and the expression of proteins essential for the initiation of DNA replication...
  8. ncbi E2F-6: a novel member of the E2F family is an inhibitor of E2F-dependent transcription
    P Cartwright
    European Institute of Oncology, Department of Experimental Oncology, Milan, Italy
    Oncogene 17:611-23. 1998
    ..Our data suggest that E2F-6 expression delays the exit from S-phase rather than inducing S-phase, which further emphasizes the functional difference between E2F-6 and the previously known E2F family members...
  9. ncbi The E2F transcription factors: key regulators of cell proliferation
    H Muller
    European Institute of Oncology, Department of Experimental Oncology, Via Ripamonti 435, 20141, Milan, Italy
    Biochim Biophys Acta 1470:M1-12. 2000
    ..We will discuss the relevance of a thorough understanding of E2F function for cancer therapy...
  10. ncbi Phosphorylation of mammalian CDC6 by cyclin A/CDK2 regulates its subcellular localization
    B O Petersen
    European Institute of Oncology, Department of Experimental Oncology, Via Ripamonti 435, 20141 Milan, Italy
    EMBO J 18:396-410. 1999
    ..Based on our data we suggest that the phosphorylation of CDC6 by Cyclin A/CDK2 is a negative regulatory event that could be implicated in preventing re-replication during S phase and G2...
  11. ncbi The promyelocytic leukemia gene product (PML) forms stable complexes with the retinoblastoma protein
    M Alcalay
    Department of Experimental Oncology, European Institute of Oncology, Milan, Italy
    Mol Cell Biol 18:1084-93. 1998
    ..Our results suggest that PML may be part of transcription-regulatory complexes and that the oncogenic potential of the PML-RAR alpha protein may derive from the alteration of PML-regulated transcription...
  12. ncbi Induction of S-phase entry by E2F transcription factors depends on their nuclear localization
    H Muller
    Department of Experimental Oncology, European Institute of Oncology, Milan, Italy
    Mol Cell Biol 17:5508-20. 1997
    ....
  13. ncbi The regulation of APAF1 expression during development and tumourigenesis
    E S Hickman
    European Institute of Oncology, Department of Experimental Oncology, Via Ripamonti 435, 20141 Milan, Italy
    Apoptosis 7:167-71. 2002
    ..In this review we will focus on the regulation of APAF1 expression, in particular with regard to recent developments in our understanding of the role of APAF1 in both tumourigenesis and mammalian development...
  14. ncbi E2F1-mediated transcriptional inhibition of the plasminogen activator inhibitor type 1 gene
    M Koziczak
    Friedrich Miescher Institute, Basel, Switzerland
    Eur J Biochem 268:4969-78. 2001
    ..5' deletion analysis of the PAI-1 promoter showed that multiple sites were responsible for the E2F negative regulation, some of which were promoter dependent. Interestingly, one of these sites is a p53-binding element...
  15. ncbi Heterodimerization of the transcription factors E2F-1 and DP-1 leads to cooperative trans-activation
    K Helin
    Massachusetts General Hospital Cancer Center, Charlestown 02129
    Genes Dev 7:1850-61. 1993
    ..We suggest that "E2F" is the activity that is formed when an E2F-1-related protein and a DP-1-related protein dimerize...
  16. ncbi A cDNA encoding a pRB-binding protein with properties of the transcription factor E2F
    K Helin
    Massachusetts General Hospital, Charlestown 02129
    Cell 70:337-50. 1992
    ..These properties suggest that RBP3 encodes E2F, or an E2F-like protein...
  17. ncbi Analysis of p107-associated proteins: p107 associates with a form of E2F that differs from pRB-associated E2F-1
    N Dyson
    Massachusetts General Hospital Cancer Center, Charlestown 02129
    J Virol 67:7641-7. 1993
    ..The 62- to 65-kDa proteins have many of the properties of the transcription factor E2F but were distinguished from pRB-associated E2F-1 by both immunologic and biochemical properties...
  18. ncbi The retinoblastoma protein binds to a family of E2F transcription factors
    J A Lees
    Massachusetts General Hospital Cancer Center, Charlestown 02129
    Mol Cell Biol 13:7813-25. 1993
    ..These observations suggest that the E2F activities described previously result from the combined action of a family of proteins...
  19. ncbi Inhibition of E2F-1 transactivation by direct binding of the retinoblastoma protein
    K Helin
    Massachusetts General Hospital Cancer Center, Charlestown 02129
    Mol Cell Biol 13:6501-8. 1993
    ..In conclusion, these data demonstrate that pRB inhibits E2F-dependent transactivation by direct protein-protein interaction...
  20. ncbi Inhibition of cell proliferation by p107, a relative of the retinoblastoma protein
    L Zhu
    Massachusetts General Hospital Cancer Center, Charlestown 02129
    Genes Dev 7:1111-25. 1993
    ..Together, these results suggest a biological role of p107 that is related, but not identical, to that of pRB...
  21. ncbi A conserved TATA-less proximal promoter drives basal transcription from the urokinase-type plasminogen activator receptor gene
    E Soravia
    Department of Genetics and Microbiology, University of Milano, Italy
    Blood 86:624-35. 1995
    ..A subregion of this sequence, highly conserved between human and murine genes, contains most of the promoter activity and is specifically bound by HeLa nuclear proteins, one of which belongs to the SP1 class...
  22. ncbi E2F-1-induced p53-independent apoptosis in transgenic mice
    C Holmberg
    Department of Molecular Cell Biology, Institute of Molecular Biology, University of Copenhagen, Denmark
    Oncogene 17:143-55. 1998
    ..Testicular atrophy as a result of overexpression of E2F-1 and DP-1 is independent of functional p53, since p53-nullizygous transgenic mice overexpressing E2F-1 and DP-1 also suffered testicular atrophy...
  23. ncbi Characterization of the retinoblastoma binding proteins RBP1 and RBP2
    A R Fattaey
    Massachusetts General Hospital Cancer Center, Charlestown 02129
    Oncogene 8:3149-56. 1993
    ..In addition we have been able to identify complexes of pRB and RBP1 in vivo that are dissociated in the presence of purified human papillomavirus E7 protein...
  24. ncbi Cyclin E-induced S phase without activation of the pRb/E2F pathway
    J Lukas
    Danish Cancer Society, Division of Cancer Biology, Copenhagen
    Genes Dev 11:1479-92. 1997
    ..They furthermore indicate that a lack of E2F-mediated transactivation can be compensated by hyperactivation of this cyclin E-controlled event...
  25. ncbi Ataxin-1 with an expanded glutamine tract alters nuclear matrix-associated structures
    P J Skinner
    Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis 55455, USA
    Nature 389:971-4. 1997
    ..We therefore propose that a critical aspect of SCA1 pathogenesis involves the disruption of a nuclear matrix-associated domain...
  26. ncbi E2F-HDAC complexes negatively regulate the tumor suppressor gene ARHI in breast cancer
    Z Lu
    Department of Experimental Therapeutics, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA
    Oncogene 25:230-9. 2006
    ..Taken together, our results suggest that E2F1, 4 and their complexes with HDAC play an important role in downregulating the expression of the tumor suppressor gene ARHI in breast cancer cells...