Research Topics
Genomes and Genes | S GuglielmettiSummaryAffiliation: University of Milan Country: Italy Publications
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Detail Information
Publications
Molecular characterization of Bifidobacterium longum biovar longum NAL8 plasmids and construction of a novel replicon screening systemSimone Guglielmetti
Department of Food Science and Microbiology, Industrial Microbiology Section, University of Milan, Via Celoria 2, 20133 Milan, Italy
Appl Microbiol Biotechnol 74:1053-61. 2007..As a result, we developed a system suitable for avoiding possible interference by other functional replication modules on the vector and for screening functional replicons from wild-type plasmids...
Biotransformation strategy to reduce allergens in propolisClaudio Gardana
Division of Nutrition, Universita degli Studi di Milano, Milan, Italy
Appl Environ Microbiol 78:4654-8. 2012..This is the first study demonstrating that the allergenic caffeate esters of propolis can be eliminated by means of a bacterial biotransformation procedure...
In vitro functional and immunomodulatory properties of the Lactobacillus helveticus MIMLh5-Streptococcus salivarius ST3 association that are relevant to the development of a pharyngeal probiotic productValentina Taverniti
Department of Food Science and Microbiology, Universita degli Studi di Milano, Milan, Italy
Appl Environ Microbiol 78:4209-16. 2012..In conclusion, we propose the combined use of L. helveticus MIMLh5 and S. salivarius ST3 for the preparation of novel products that display probiotic properties for the pharyngeal mucosa...
Implication of an outer surface lipoprotein in adhesion of Bifidobacterium bifidum to Caco-2 cellsSimone Guglielmetti
Department of Food Science and Microbiology, University of Milan, Via Celoria 2, 20133 Milan, Italy
Appl Environ Microbiol 74:4695-702. 2008..BopA is the first protein described to date to be directly involved in the adhesion of bifidobacteria to Caco-2 cells and to show immunomodulatory activity...
Bacterial cinnamoyl esterase activity screening for the production of a novel functional food productSimone Guglielmetti
Department of Food Science and Microbiology, Industrial Microbiology Section, University of Milan, Via Celoria 2, 20133 Milan, Italy
Appl Environ Microbiol 74:1284-8. 2008..The novel food product was demonstrated to display high total antioxidant power and potential probiotic properties...
Study of the adhesion of Bifidobacterium bifidum MIMBb75 to human intestinal cell linesSimone Guglielmetti
Department of Food Science and Microbiology, University of Milan, Milan, Italy
Curr Microbiol 59:167-72. 2009..bifidum to only restricted distal sites of the gut...
Small rolling circle plasmids in Bacillus subtilis and related species: organization, distribution, and their possible role in host physiologyS Guglielmetti
Department of Food Science and Microbiology, Industrial Microbiology Section, University of Milan, Via Celoria 2, Milan, Italy
Plasmid 57:245-64. 2007..Finally, the parasitic vs. mutualistic nature of small rolling circle plasmids is briefly discussed...
Construction, characterization and exemplificative application of bioluminescent Bifidobacterium longum biovar longumSimone Guglielmetti
Department of Food Science and Microbiology, University of Milan, Italy
Int J Food Microbiol 124:285-90. 2008..In sum, our results show that bioluminescent B. longum, transformed with the pGBL8b plasmid, is a valuable tool for rapidly studying the physiological state of anaerobic bacterial cells under different environmental conditions...
Oral bacteria as potential probiotics for the pharyngeal mucosaSimone Guglielmetti
Dipartimento di Scienze e Tecnologie Alimentari, Universita degli Studi di Milano, Via Celoria 2, 20133 Milan, Italy
Appl Environ Microbiol 76:3948-58. 2010..They could display a good degree of adaptation to the host and possess potential immunomodulatory and anti-inflammatory properties...
A dairy bacterium displays in vitro probiotic properties for the pharyngeal mucosa by antagonizing group A streptococci and modulating the immune responseSimone Guglielmetti
Dipartimento di Scienze e Tecnologie Alimentari, Universita degli Studi di Milano, Milano, Italy
Infect Immun 78:4734-43. 2010..In conclusion, we propose MIMLh5 as a potential probiotic bacterium for the human pharynx, with promising antagonistic and immunomodulatory properties...
Randomised clinical trial: Bifidobacterium bifidum MIMBb75 significantly alleviates irritable bowel syndrome and improves quality of life--a double-blind, placebo-controlled studyS Guglielmetti
Department of Food Science and Microbiology, Universita degli Studi di Milano, Via Celoria 2, Milan, Italy
Aliment Pharmacol Ther 33:1123-32. 2011..As probiotics have been reported to restore the intestinal microbiota and the gut barrier, the therapeutic potential of probiotics within IBS became of strong interest...
Genetic relationship among Bacillus licheniformis rolling-circle-replicating plasmids and complete nucleotide sequence of pBL63.1, an atypical repliconS Guglielmetti
Department of Food Science and Microbiology, Industrial Microbiology Section, University of Milan, Via Celoria 2, 20133 Milan, Italy
Plasmid 54:93-103. 2005..pBL63.1 represents an exception to the low-level diversity hypothesis among Bacillus RC replicons...
Conditions affecting cell surface properties of human intestinal bifidobacteriaEnrica Canzi
Department of Food Science and Microbiology, University of Milan, Via Celoria 2, 20133 Milan, Italy
Antonie Van Leeuwenhoek 88:207-19. 2005..adolescentis strains, were strictly dependent on the pH of the medium. Moreover, protease treatment experiments suggested that proteins had a key role in the autoaggregating ability of B. bifidum and B. adolescentis strains...
16S-23S rRNA intergenic spacer region sequence variation in Streptococcus thermophilus and related dairy streptococci and development of a multiplex ITS-SSCP analysis for their identificationDiego Mora
Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, sezione Microbiologia Industriale, Universita degli Studi di Milano, Milano, Italy
Microbiology 149:807-13. 2003..thermophilus and S. salivarius strains. With the aim of developing a rapid tool for the identification of the dairy streptococci species a multiplex ITS-SSCP analysis of two discrete regions within the ITS locus was carried out...
Unusual organization for lactose and galactose gene clusters in Lactobacillus helveticusMaria Grazia Fortina
Industrial Microbiology Section, Department of Food Science and Microbiology, University of Milan, 20133 Milan, Italy
Appl Environ Microbiol 69:3238-43. 2003..In the presence of glucose and galactose galE was transcribed from its promoter, suggesting that the corresponding enzyme can be expressed constitutively. Lactose transport was inducible by addition of lactose to the growth medium...
Homologies among three Bacillus licheniformis plasmids and molecular characterization of their replication moduleCarlo Parini
Department of Food Science and Microbiology, Industrial Microbiology Section, University of Milan, Via Celoria 2, Milan 20133, Italy
Microbiol Res 159:211-7. 2004..This investigation provides more information concerning phylogeny, interrelationships and level of diversity among Bacillus plasmids and a molecular characterization of three plasmids useful for the construction of cloning vectors...
Urease biogenesis in Streptococcus thermophilusDiego Mora
Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, Universita degli Studi di Milano, Via Celoria 2, 20133 Milan, Italy
Res Microbiol 156:897-903. 2005..thermophilus is regulated differently compared with that of the closely related S. salivarius. These differences are discussed taking into consideration the different habitats colonized by the two bacterial species...
Aspartate biosynthesis is essential for the growth of Streptococcus thermophilus in milk, and aspartate availability modulates the level of urease activityStefania Arioli
Department of Food Science and Microbiology, University of Milan, Via Celoria 2, 20133 Milano, Italy
Appl Environ Microbiol 73:5789-96. 2007....
Carbamoylphosphate synthetase activity is essential for the optimal growth of Streptococcus thermophilus in milkS Arioli
Department of Food Science and Microbiology, University of Milan, Milan, Italy
J Appl Microbiol 107:348-54. 2009..The aim of the study was to study the role of carbon dioxide metabolism in Streptococcus thermophilus through investigation of the phenotype of a carbamoylphosphate synthetase-negative mutant...
Complete sequence and structural organization of pFL5 and pFL7, two cryptic plasmids from Bacillus licheniformisC Parini
Department of Food Science and Microbiology, University of Milan, Via Celoria 2, 20133, Italy
Plasmid 51:192-202. 2004..These plasmids seem to be related to plasmids identified within the Bacillus subtilis group, confirming the low-level diversity among these replicons...
Generation and comparison of bioluminescent and fluorescent Bacillus licheniformisIsabella Tamagnini
Department of Food Science and Microbiology, University of Milan, Via Celoria 2, Milan, Italy
Curr Microbiol 57:245-50. 2008..Thus, the light emission was found to be a reliable, sensitive, and real-time indicator of the "well-being" of cells, whereas fluorescence allowed one to visualize both metabolically active and inactive cells...
The relevance of carbon dioxide metabolism in Streptococcus thermophilusStefania Arioli
Department of Food Science and Microbiology, Milan, Italy
Microbiology 155:1953-65. 2009..Our results reveal the capnophilic nature of this micro-organism, underlining the essential role of CO(2) in S. thermophilus physiology, and suggesting potential applications in dairy fermentation processes...
Complete nucleotide sequence of pGS18, a 62.8-kb plasmid from Geobacillus stearothermophilus strain 18Milda Stuknyte
Department of Plant Physiology and Microbiology, Faculty of Natural Sciences, Vilnius University, Ciurlionio 21 27, 03101 Vilnius, Lithuania
Extremophiles 12:415-29. 2008....
