Claudio O Gualerzi

Summary

Affiliation: University of Camerino
Country: Italy

Publications

  1. ncbi request reprint Selective expression of the beta-subunit of nucleoid-associated protein HU during cold shock in Escherichia coli
    Mara Giangrossi
    Laboratory of Genetics, Department of Biology MCA, University of Camerino, Camerino MC, Italy
    Mol Microbiol 44:205-16. 2002
  2. ncbi request reprint Transcriptional and post-transcriptional control of cold-shock genes
    Claudio O Gualerzi
    Laboratory of Genetics, Department of Biology MCA, University of Camerino, 62032 Camerino MC, Italy
    J Mol Biol 331:527-39. 2003
  3. pmc Characterization of GE82832, a peptide inhibitor of translocation interacting with bacterial 30S ribosomal subunits
    Letizia Brandi
    Laboratory of Genetics, Department of Biology MCA, University of Camerino, Italy
    RNA 12:1262-70. 2006
  4. pmc Translation initiation without IF2-dependent GTP hydrolysis
    Attilio Fabbretti
    Laboratory of Genetics, Department of Biosciences and Biotechnology, University of Camerino, 62032 Camerino, Macerata, Italy
    Nucleic Acids Res 40:7946-55. 2012
  5. ncbi request reprint Cold-shock-induced de novo transcription and translation of infA and role of IF1 during cold adaptation
    Mara Giangrossi
    Laboratory of Genetics, Department of Biology MCA, University of Camerino, 62032 Camerino MC, Italy
    Mol Microbiol 64:807-21. 2007
  6. doi request reprint Ribosomal interaction of Bacillus stearothermophilus translation initiation factor IF2: characterization of the active sites
    Enrico Caserta
    Laboratory of Genetics, Department of Biology, University of Camerino, 62032 Camerino MC, Italy
    J Mol Biol 396:118-29. 2010
  7. ncbi request reprint The real-time path of translation factor IF3 onto and off the ribosome
    Attilio Fabbretti
    Laboratory of Genetics, Department of Biology, University of Camerino, 62032 Camerino, MC, Italy
    Mol Cell 25:285-96. 2007
  8. pmc The nucleotide-binding site of bacterial translation initiation factor 2 (IF2) as a metabolic sensor
    Pohl Milon
    Laboratory of Genetics, Department of Biology MCA, University of Camerino, 62032 Camerino MC, Italy
    Proc Natl Acad Sci U S A 103:13962-7. 2006
  9. ncbi request reprint The translation initiation functions of IF2: targets for thiostrepton inhibition
    Letizia Brandi
    Laboratory of Genetics, Department of Biology MCA, University of Camerino, 62032 Camerino MC, Italy
    J Mol Biol 335:881-94. 2004
  10. ncbi request reprint Environmental control of the in vivo oligomerization of nucleoid protein H-NS
    Stefano Stella
    Laboratory of Genetics, Department of Biology MCA, University of Camerino, 62032 Camerino MC, Italy
    J Mol Biol 355:169-74. 2006

Collaborators

  • Sylvie Rimsky
  • Anna Brandi
  • Stefano Donadio
  • Marina V Rodnina
  • Rolf Wagner
  • Benoit Masquida
  • Jordi Vila
  • Linda Cavaletti
  • Pascale Romby
  • Alessandra Marazzi
  • M Madan Babu
  • Andrew Travers
  • Margherita Sosio
  • Attilio Fabbretti
  • Letizia Brandi
  • Cynthia L Pon
  • Anna Maria Giuliodori
  • Pohl Milon
  • Roberto Spurio
  • Enrico Caserta
  • Anna La Teana
  • Stefano Stella
  • Mara Giangrossi
  • Stefano Marzi
  • Stavroula Kapralou
  • Jerneja Tomsic
  • Dezemona Petrelli
  • Monica Abbondi
  • J Stephen Lodmell
  • Walter E Hill
  • Maurizio Falconi
  • Thi Nhu Mai Nguyen
  • Chiara Garulli
  • Sonja Grill
  • Albert E Dahlberg
  • Isabella Moll
  • Andrey L Konevega
  • Benjamin Lang
  • Daniele Losi
  • Ameriga Lazzarini
  • Alexander G Myasnikov
  • Matilde Lammi
  • Cristina Madrid
  • Udo Blasi
  • Fabio Quaglia
  • Nilo R Castañedo
  • Sonia Maffioli
  • Ricardo Medina
  • Marco Sette
  • Marco Berzano
  • Alessandra Rocchetti
  • Carlotta Ferrara
  • Fabio Di Pietro
  • John Dresios
  • Emeline Bouffartigues
  • Marcel Geertz
  • Nicolas Blot
  • Ramesh Mavathur
  • Georgi Muskhelishvili
  • Scott P Hennelly
  • Malcolm Buckle
  • Marcello Carotti
  • Frank Peske
  • Sara Soto
  • María M Tavío
  • Michele Di Stefano
  • Alessandro Maio
  • Luciano Gastaldo
  • Flavia Marinelli
  • Ismaela Ciciliato
  • Emiliana Corti
  • Luigi Colombo
  • Rolf Boelens
  • Marina Feroggio
  • Gert Folkers
  • Enrico Selva
  • Eugene Tischenko
  • Marat Yusupov
  • Gulnara Yusupova
  • Bruno P Klaholz
  • Angelita Simonetti
  • Carola Fleischer
  • Natalia Soboleva
  • Cristiana Garofalo
  • William Knight
  • Jose M Nieto
  • Sonia Paytubi
  • Antonio Juárez

Detail Information

Publications38

  1. ncbi request reprint Selective expression of the beta-subunit of nucleoid-associated protein HU during cold shock in Escherichia coli
    Mara Giangrossi
    Laboratory of Genetics, Department of Biology MCA, University of Camerino, Camerino MC, Italy
    Mol Microbiol 44:205-16. 2002
    ....
  2. ncbi request reprint Transcriptional and post-transcriptional control of cold-shock genes
    Claudio O Gualerzi
    Laboratory of Genetics, Department of Biology MCA, University of Camerino, 62032 Camerino MC, Italy
    J Mol Biol 331:527-39. 2003
    ....
  3. pmc Characterization of GE82832, a peptide inhibitor of translocation interacting with bacterial 30S ribosomal subunits
    Letizia Brandi
    Laboratory of Genetics, Department of Biology MCA, University of Camerino, Italy
    RNA 12:1262-70. 2006
    ....
  4. pmc Translation initiation without IF2-dependent GTP hydrolysis
    Attilio Fabbretti
    Laboratory of Genetics, Department of Biosciences and Biotechnology, University of Camerino, 62032 Camerino, Macerata, Italy
    Nucleic Acids Res 40:7946-55. 2012
    ..Our data underlie the importance of GTP as an IF2 ligand in the early initiation steps and the dispensability of the free energy generated by the IF2 GTPase in the late events of the translation initiation pathway...
  5. ncbi request reprint Cold-shock-induced de novo transcription and translation of infA and role of IF1 during cold adaptation
    Mara Giangrossi
    Laboratory of Genetics, Department of Biology MCA, University of Camerino, 62032 Camerino MC, Italy
    Mol Microbiol 64:807-21. 2007
    ..The increased level of IF1 during cold adaptation is essential for overcoming the higher stability of the 70S monomers at low temperature and for providing a sufficient pool of dissociated 30S subunits capable of initiating translation...
  6. doi request reprint Ribosomal interaction of Bacillus stearothermophilus translation initiation factor IF2: characterization of the active sites
    Enrico Caserta
    Laboratory of Genetics, Department of Biology, University of Camerino, 62032 Camerino MC, Italy
    J Mol Biol 396:118-29. 2010
    ..It is noteworthy that the E424K mutant, which completely lacks GTPase activity, displays IF2 wild-type capacity in supporting initiation of dipeptide formation...
  7. ncbi request reprint The real-time path of translation factor IF3 onto and off the ribosome
    Attilio Fabbretti
    Laboratory of Genetics, Department of Biology, University of Camerino, 62032 Camerino, MC, Italy
    Mol Cell 25:285-96. 2007
    ..Upon 30S-50S association, IF3 dissociates concomitantly with the establishment of the 30S-50S bridges, following the reverse path of its binding with the IF3N-A790 interaction being lost before the IF3C-G700 interaction...
  8. pmc The nucleotide-binding site of bacterial translation initiation factor 2 (IF2) as a metabolic sensor
    Pohl Milon
    Laboratory of Genetics, Department of Biology MCA, University of Camerino, 62032 Camerino MC, Italy
    Proc Natl Acad Sci U S A 103:13962-7. 2006
    ....
  9. ncbi request reprint The translation initiation functions of IF2: targets for thiostrepton inhibition
    Letizia Brandi
    Laboratory of Genetics, Department of Biology MCA, University of Camerino, 62032 Camerino MC, Italy
    J Mol Biol 335:881-94. 2004
    ..Overall, our data indicate that the translation initiation function of IF2 is as sensitive as the translocation function of EF-G to thiostrepton inhibition...
  10. ncbi request reprint Environmental control of the in vivo oligomerization of nucleoid protein H-NS
    Stefano Stella
    Laboratory of Genetics, Department of Biology MCA, University of Camerino, 62032 Camerino MC, Italy
    J Mol Biol 355:169-74. 2006
    ....
  11. ncbi request reprint Translation initiation factor IF2 interacts with the 30 S ribosomal subunit via two separate binding sites
    Enrico Caserta
    Laboratory of Genetics, Department of Biology, University of Camerino, 62032 Camerino MC, Italy
    J Mol Biol 362:787-99. 2006
    ....
  12. pmc Cold-stress-induced de novo expression of infC and role of IF3 in cold-shock translational bias
    Anna Maria Giuliodori
    Laboratory of Genetics, Department of Biology MCA, University of Camerino, Camerino, Italy
    RNA 13:1355-65. 2007
    ....
  13. doi request reprint Structural and functional characterization of the bacterial translocation inhibitor GE82832
    Letizia Brandi
    Department of Biosciences and Biotechnology, University of Camerino, Camerino MC, Italy
    FEBS Lett 586:3373-8. 2012
    ....
  14. pmc Translation initiation factor IF1 of Bacillus stearothermophilus and Thermus thermophilus substitute for Escherichia coli IF1 in vivo and in vitro without a direct IF1-IF2 interaction
    Stavroula Kapralou
    Laboratory of Genetics, Department of Biology MCA, University of Camerino, 62032 Camerino MC, Italy
    Mol Microbiol 70:1368-77. 2008
    ..However, the thermophilic factors do not stimulate ribosomal binding of IF2DeltaN, regardless of 30S subunits and IF2 origin. We conclude that an IF1-IF2 interaction is not universally conserved and is not essential for cell survival...
  15. ncbi request reprint Novel tetrapeptide inhibitors of bacterial protein synthesis produced by a Streptomyces sp
    Letizia Brandi
    University of Camerino, Via Camerini 2, 62032 Camerino, Italy
    Biochemistry 45:3692-702. 2006
    ..The novelty of the chemical structure and of the specific mode of action on the initiation phase of bacterial protein synthesis makes GE81112 a unique scaffold for designing new drugs...
  16. doi request reprint Kinetic checkpoint at a late step in translation initiation
    Pohl Milon
    Department of Biology MCA, Laboratory of Genetics, University of Camerino, 62032 Camerino, Italy
    Mol Cell 30:712-20. 2008
    ....
  17. doi request reprint Characterization of Bacillus stearothermophilus infA and of its product IF1
    Stavroula Kapralou
    Laboratory of Genetics, Department of Biology MCA, University of Camerino, Camerino MC, Italy
    Gene 428:31-5. 2009
    ..stearothermophilus IF1 now allows us to analyze the translation initiation pathway using efficient in vitro tests based entirely on purified components derived from this thermophilic Gram-positive bacterium...
  18. doi request reprint The cspA mRNA is a thermosensor that modulates translation of the cold-shock protein CspA
    Anna Maria Giuliodori
    Laboratory of Genetics, Department of Biology MCA, University of Camerino, 62032 Camerino MC, Italy
    Mol Cell 37:21-33. 2010
    ..Unlike with other previously studied RNA thermometers, these structural rearrangements do not result from melting of hairpin structures...
  19. ncbi request reprint Transient kinetics, fluorescence, and FRET in studies of initiation of translation in bacteria
    Pohl Milon
    Laboratory of Genetics, Department of Biology MCA, University of Camerino, Camerino, Italy
    Methods Enzymol 430:1-30. 2007
    ..The methods described here are based on results obtained with initiation components from Escherichia coli but can be adopted for mechanistic studies of initiation in other prokaryotic or eukaryotic systems...
  20. pmc Specific, efficient, and selective inhibition of prokaryotic translation initiation by a novel peptide antibiotic
    Letizia Brandi
    Laboratory of Genetics, Department of Biology MCA, University of Camerino, 62032 Camerino MC, Italy
    Proc Natl Acad Sci U S A 103:39-44. 2006
    ..In vitro GE81112 was found to target the 30S ribosomal subunit and to interfere with both coded and noncoded P-site binding of fMet-tRNA, thereby selectively inhibiting formation of the 30S initiation complex...
  21. ncbi request reprint Real-time dynamics of ribosome-ligand interaction by time-resolved chemical probing methods
    Attilio Fabbretti
    Department of Biology MCA, University of Camerino, Camerino, Italy
    Methods Enzymol 430:45-58. 2007
    ..These methods allow the investigation of the path and dynamics, in a approximately equal 50 to 1500ms time range, of the binding and dissociation of ribosomal ligands...
  22. pmc Nature and mechanism of the in vivo oligomerization of nucleoid protein H-NS
    Stefano Stella
    Department of Biology MCA, Laboratory of Genetics, University of Camerino, Camerino MC, Italy
    EMBO J 24:2896-905. 2005
    ..A model describing H-NS dimerization and tetramerization based on all available data and suggesting the existence in the tetramer of a bundle of four alpha-helices, each contributed by an H-NS monomer, is presented...
  23. pmc Preferential translation of cold-shock mRNAs during cold adaptation
    Anna Maria Giuliodori
    Laboratory of Genetics, Department of Biology MCA, University of Camerino, 62032 Camerino MC, Italy
    RNA 10:265-76. 2004
    ..Finally, in contrast to a previous claim, translation of cold-shock cspA mRNA in the cold was found to be as sensitive as that of a non-cold-shock mRNA to both chloramphenicol and kanamycin inhibition...
  24. ncbi request reprint Mapping the active sites of bacterial translation initiation factor IF3
    Dezemona Petrelli
    Laboratory of Genetics, Department of Biology MCA, University of Camerino, 62032 Camerino, Italy
    J Mol Biol 331:541-56. 2003
    ..Overall, the results define two active surfaces in IF3C, and indicate that the different functions of IF3 rely on different molecular mechanisms involving separate active sites...
  25. doi request reprint Assays for the identification of inhibitors targeting specific translational steps
    Letizia Brandi
    Department of Biology MCA, University of Camerino, Camerino, MC, Italy
    Methods Mol Med 142:87-105. 2008
    ..When applied to the high-throughput screening (HTS) of a library of approximately 25,000 natural products, this assay led to the identification of two novel and specific inhibitors of bacterial translation...
  26. pmc The antibiotic Furvina® targets the P-site of 30S ribosomal subunits and inhibits translation initiation displaying start codon bias
    Attilio Fabbretti
    Laboratory of Genetics, Department of Biosciences and Biotechnology, University of Camerino, 62032 Camerino, MC, Italy
    Nucleic Acids Res 40:10366-74. 2012
    ..This codon discrimination by G1 is reminiscent, though of opposite type of that displayed by IF3 in its fidelity function, and remarkably does not occur in the absence of this factor...
  27. ncbi request reprint Review on bacterial stress topics
    Anna Maria Giuliodori
    Department of Biology, University of Camerino, Italy
    Ann N Y Acad Sci 1113:95-104. 2007
    ..The strategy developed by E. coli to reach this goal consists in the induction of a set of (cold-shock) genes whose expression is regulated at both transcriptional and posttranscriptional levels...
  28. doi request reprint How to cope with the quest for new antibiotics
    Attilio Fabbretti
    Laboratory of Genetics, Department of Biosciences and Biotechnology, University of Camerino, Camerino MC, Italy
    FEBS Lett 585:1673-81. 2011
    ..Here we outline some strategies that could help meet this emergency and list promising new targets...
  29. pmc Ribosomal localization of translation initiation factor IF2
    Stefano Marzi
    Laboratory of Genetics, Department of Biology MCA, University of Camerino, 62032 Camerino MC Italy
    RNA 9:958-69. 2003
    ..Comparison of our present findings with previous data further suggests that the IF2 orientation on the 30S subunit changes during the transition from the 30S to 70S initiation complex...
  30. doi request reprint Development of molecular tools for the detection of freshwater diatoms
    Thi Nhu Mai Nguyen
    Department of Biosciences and Biotechnology, University of Camerino, Via Gentile III da Varano, 62032 Camerino, Italy
    J Microbiol Methods 84:33-40. 2011
    ..This study provides experimental conditions for a fast and accurate detection of diatoms, and demonstrates the feasibility of the use of molecular tools in the evaluation of water quality...
  31. pmc Temperature- and H-NS-dependent regulation of a plasmid-encoded virulence operon expressing Escherichia coli hemolysin
    Cristina Madrid
    Departament de Microbiologia, Universitat de Barcelona, 08028 Barcelona, Spain
    J Bacteriol 184:5058-66. 2002
    ..We present a model to explain the temperature-modulated repression of the hly operon, based on the experiments reported here and other, preexisting data...
  32. ncbi request reprint Temperature-dependent translation of leaderless and canonical mRNAs in Escherichia coli
    Sonja Grill
    Institute of Microbiology and Genetics, Vienna Biocenter, University of Vienna, Dr Bohrgasse 9, 1030 Vienna, Austria
    FEMS Microbiol Lett 211:161-7. 2002
    ....
  33. ncbi request reprint Targets and assays for discovering novel antibacterial agents
    Stefano Donadio
    Biosearch Italia, Via R Lepetit 34, 21040 Gerenzano VA, Italy
    J Biotechnol 99:175-85. 2002
    ..Two assay strategies have been implemented: a pathway-based assay, where a series of essential bacterial targets is screened in a single assay; and a binding assay, where many targets can be screened individually in the same format...
  34. ncbi request reprint Methods for identifying compounds that specifically target translation
    Letizia Brandi
    Biotechnomics, Insubrias BioPark, Gerenzano, Italy
    Methods Enzymol 431:229-67. 2007
    ..Other methods presented here are suitable for secondary screening tests aimed at identifying a specific target of an antibiotic within the translational pathway of prokaryotic cells...
  35. ncbi request reprint Conformational transition of initiation factor 2 from the GTP- to GDP-bound state visualized on the ribosome
    Alexander G Myasnikov
    Department of Structural Biology and Genomics, Institute of Genetics and Molecular and Cellular Biology, Centre National de la Recherche Scientifique Institut National de la Santé et de la Recherche Médicale, Universite Louis Pasteur, Illkirch, France
    Nat Struct Mol Biol 12:1145-9. 2005
    ..Our data also provide insights into the molecular mechanism guiding release of IF1 and IF3...
  36. pmc High-affinity DNA binding sites for H-NS provide a molecular basis for selective silencing within proteobacterial genomes
    Benjamin Lang
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Nucleic Acids Res 35:6330-7. 2007
    ....
  37. ncbi request reprint Initiation of protein synthesis: a target for antimicrobials
    Letizia Brandi
    Insubrias BioPark, Gerenzano VA, Italy
    Expert Opin Ther Targets 12:519-34. 2008
    ..A large amount of detailed mechanistic and structural information on the bacterial translation initiation apparatus has been uncovered in recent years...
  38. ncbi request reprint Leaderless mRNAs in bacteria: surprises in ribosomal recruitment and translational control
    Isabella Moll
    Institute of Microbiology and Genetics, Vienna Biocenter, 1030 Vienna, Austria
    Mol Microbiol 43:239-46. 2002
    ..In fact, the faithful translation of leaderless mRNAs in heterologous systems shows that the ability to translate leaderless mRNAs is an evolutionarily conserved function of the translational apparatus...