Research Topics
| Giorgio DieciSummaryAffiliation: University of Parma Country: Italy Publications
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Detail Information
Publications
Transcription reinitiation by RNA polymerase IIIGiorgio Dieci
Dipartimento di Bioscienze, Universita degli Studi di Parma, Parco Area delle Scienze 23 A, 43124 Parma, Italy
Biochim Biophys Acta 1829:331-41. 2013..This article is part of a Special Issue entitled: Transcription by Odd Pols...
Identification of RNA polymerase III-transcribed genes in eukaryotic genomesGiorgio Dieci
Dipartimento di Bioscienze, Universita degli Studi di Parma, Parco Area delle Scienze 23 A, 43124 Parma, Italy
Biochim Biophys Acta 1829:296-305. 2013..This article is part of a Special Issue entitled: Transcription by Odd Pols...
Eukaryotic snoRNAs: a paradigm for gene expression flexibilityGiorgio Dieci
Dipartimento di Biochimica e Biologia Molecolare, Universita degli Studi di Parma, Parma, Italy
Genomics 94:83-8. 2009..We finally discuss the relevance of snoRNA genomic studies for our general understanding of ncRNA family evolution and expression in eukaryotes...
Positive modulation of RNA polymerase III transcription by ribosomal proteinsGiorgio Dieci
Dipartimento di Biochimica e Biologia Molecolare, Universita degli Studi di Parma, Viale G P Usberti 23 A, 43100 Parma, Italy
Biochem Biophys Res Commun 379:489-93. 2009....
The expanding RNA polymerase III transcriptomeGiorgio Dieci
Dipartimento di Biochimica e Biologia Molecolare, Universita degli Studi di Parma, Viale G P Usberti 23 A, 43100 Parma, Italy
Trends Genet 23:614-22. 2007..The extent of the Pol III transcriptome, the complexity of its regulation and its influence on cell physiology, development and disease are emerging as new areas for future research...
Distinct modes of TATA box utilization by the RNA polymerase III transcription machineries from budding yeast and higher plantsGiorgio Dieci
Department of Biochemistry and Molecular Biology, University of Parma, Parco Area delle Scienze 23A, 43100 Parma, Italy
Gene 379:12-25. 2006..Interactions involving components other than TBP are thus responsible for the strong TATA box requirement of plant tDNA transcription...
A general procedure for the production of antibody reagents against eukaryotic ribosomal proteinsGiorgio Dieci
Dipartimento di Biochimica e Biologia Molecolare, Universita degli Studi di Parma, Parco Area delle Scienze 23 A, 43100 Parma, Italy
Protein Pept Lett 12:555-60. 2005..The specificity and sensitivity of detection of the antibodies produced by this procedure are documented...
Detours and shortcuts to transcription reinitiationGiorgio Dieci
Dipartimento di Biochimica e Biologia Molecolare, Universita di Parma, Italy
Trends Biochem Sci 28:202-9. 2003..These pathways influence the availability for reinitiation of template DNA and/or transcription proteins, and represent an important yet poorly characterized aspect of gene regulation...
Requirement of Nhp6 proteins for transcription of a subset of tRNA genes and heterochromatin barrier function in Saccharomyces cerevisiaePriscilla Braglia
Dipartimento di Biochimica e Biologia Molecolare, Universita degli Studi di Parma, Viale G P Usberti 23A, 43100 Parma, Italy
Mol Cell Biol 27:1545-57. 2007..Our data suggest that Nhp6 proteins are important components of Pol III chromatin templates that contribute both to the robustness of tRNA gene expression and to positional effects of Pol III transcription complexes...
A minimal promoter for TFIIIC-dependent in vitro transcription of snoRNA and tRNA genes by RNA polymerase IIIElisa Guffanti
Dipartimento di Biochimica e Biologia Molecolare, , 43100 Parma, Italy
J Biol Chem 281:23945-57. 2006..The box A can thus be viewed as a core TFIIIC-interacting element that, assisted by upstream TFIIIB-DNA contacts, is sufficient to promote class III gene transcription...
A composite upstream sequence motif potentiates tRNA gene transcription in yeastSilvia Giuliodori
Dipartimento di Biochimica e Biologia Molecolare, , Parco Area delle Scienze 23/A, 43100 Parma, Italy
J Mol Biol 333:1-20. 2003..Our data strongly support the notion that tRNA gene transcription in eukaryotes is modulated by composite TFIIIB binding sites that may confer responsiveness to variation in TFIIIB activity and/or concentration...
Distinct roles of transcription factors TFIIIB and TFIIIC in RNA polymerase III transcription reinitiationRoberto Ferrari
Dipartimento di Biochimica e Biologia Molecolare, , Parco Area delle Scienze 23/A, 43100 Parma, Italy
Proc Natl Acad Sci U S A 101:13442-7. 2004..The data reveal an unexpected complexity in the Pol III reinitiation mechanism and suggest the existence of a handing-back network between Pol III, TFIIIC, and TFIIIB on actively transcribed class III genes...
Nucleosome depletion activates poised RNA polymerase III at unconventional transcription sites in Saccharomyces cerevisiaeElisa Guffanti
Dipartimento di Biochimica e Biologia Molecolare, , 43100 Parma, Italy
J Biol Chem 281:29155-64. 2006..The maintenance, by eukaryotic genomes, of chromatin-repressed, non-coding transcription units has implications for both genome expression and organization...
The telomere-binding protein Tbf1 demarcates snoRNA gene promoters in Saccharomyces cerevisiaeMilena Preti
Dipartimento di Biochimica e Biologia Molecolare, Universita degli Studi di Parma, Parma, Italy
Mol Cell 38:614-20. 2010..Our data show that, besides the ribosomal protein gene regulator Rap1, a second telomere-binding protein also functions as a transcriptional regulator linked to yeast ribosome biogenesis...
RNA polymerase III transcription control elements: themes and variationsAndrea Orioli
Dipartimento di Biochimica e Biologia Molecolare, Universita degli Studi di Parma, Parco Area delle Scienze 23 A, 43124 Parma, Italy
Gene 493:185-94. 2012....
Sequence context effects on oligo(dT) termination signal recognition by Saccharomyces cerevisiae RNA polymerase IIIPriscilla Braglia
Dipartimento di Biochimica e Biologia Molecolare, , Italy
J Biol Chem 280:19551-62. 2005....
The transcription reinitiation properties of RNA polymerase III in the absence of transcription factorsRoberto Ferrari
Dipartimento di Biochimica e Biologia Molecolare, Universita degli Studi di Parma, Viale G P Usberti 23 A, 43100, Parma, Italy
Cell Mol Biol Lett 13:112-8. 2008..The data indicates that, in the absence of transcription factors, first-round transcription initiation by Pol III proceeds at a normal rate, while facilitated reinitiation during subsequent cycles is compromised...
Functional dissection of RNA polymerase III termination using a peptide nucleic acid as a transcriptional roadblockElisa Guffanti
Dipartimento di Biochimica e Biologia Molecolare and Dipartimento di Chimica Organica e Industriale, , 43100 Parma, Italy
J Biol Chem 279:20708-16. 2004....
Intragenic promoter adaptation and facilitated RNA polymerase III recycling in the transcription of SCR1, the 7SL RNA gene of Saccharomyces cerevisiaeGiorgio Dieci
Dipartimento di Biochimica e Biologia Molecolare, Universita di Parma, I 43100 Parma, Italy
J Biol Chem 277:6903-14. 2002....
Transcription reinitiation properties of bacteriophage T7 RNA polymeraseRoberto Ferrari
Dipartimento di Biochimica e Biologia Molecolare, , Parco Area delle Scienze 23/A, 43100 Parma, Italy
Biochem Biophys Res Commun 315:376-80. 2004..Our data suggest that polymerase recycling represents a critical step in T7 RNAP transcription, and that such a step may become rate-limiting for transcription at sub-saturating polymerase concentrations...
Assembly into snoRNP controls 5'-end maturation of a box C/D snoRNA in Saccharomyces cerevisiaeMilena Preti
Dipartimento di Biochimica e Biologia Molecolare, Universita degli Studi di Parma, 43100 Parma, Italy
Biochem Biophys Res Commun 351:468-73. 2006..Our data suggest a functional coupling between snoRNP assembly and 5'-end maturation of independently transcribed box C/D snoRNAs...
Visualizing RNA extrusion and DNA wrapping in transcription elongation complexes of bacterial and eukaryotic RNA polymerasesClaudio Rivetti
Department of Biochemistry and Molecular Biology, University of Parma, 43100, Parma, Italy
J Mol Biol 326:1413-26. 2003..DNA wrapping in the elongation complex suggests a possible mechanism by which the polymerase may overcome the physical barrier to transcription imposed by the nucleosomes...
Widespread occurrence of non-canonical transcription termination by human RNA polymerase IIIAndrea Orioli
Dipartimento di Biochimica e Biologia Molecolare, Universita degli Studi di Parma, Viale G P Usberti 23 A, 43100 Parma, Italy
Nucleic Acids Res 39:5499-512. 2011..The widespread occurrence of partial termination suggests that the Pol III primary transcriptome in mammals is unexpectedly enriched in 3'-trailer sequences with the potential to contribute novel functional ncRNAs...
New small nuclear RNA gene-like transcriptional units as sources of regulatory transcriptsAldo Pagano
Dipartimento di Oncologia Biologia e Genetica, Universita di Genova, Genoa, Italy
PLoS Genet 3:e1. 2007..By elucidating the function of one of them, we provide evidence for the existence of a sense/antisense-based gene-regulation network where part of the polymerase III transcriptome could control its polymerase II counterpart...
Modulation of yeast genome expression in response to defective RNA polymerase III-dependent transcriptionChristine Conesa
Service de Biochimie et Génétique Moléculaire, Bâtiment 144, CEA Saclay, 91191 Gif sur Yvette Cedex, France
Mol Cell Biol 25:8631-42. 2005..Such an effect might represent a key element in the coordinated transcriptional response of yeast cells to environmental changes...
