A Diaspro

Summary

Affiliation: University of Genoa
Country: Italy

Publications

  1. pmc Multi-photon excitation microscopy
    Alberto Diaspro
    LAMBS MicroScoBio Research Center, Department of Physics, University of Genoa, Via Dodecaneso 33, 16146 Genova, Italy
    Biomed Eng Online 5:36. 2006
  2. pmc A new FRAP/FRAPa method for three-dimensional diffusion measurements based on multiphoton excitation microscopy
    Davide Mazza
    Laboratory for Advanced Microscopy, Bioimaging, and Spectroscopy MicroSCoBiO Research Center, Department of Physics, University of Genoa, Genoa, Italy
    Biophys J 95:3457-69. 2008
  3. ncbi request reprint Cytofluorometry and fluorescence confocal laser scanning microscopy (CLSM) in the study of neutral lipid dynamics in Paramecium primaurelia mating types during cell line development
    P Ramoino
    Institute of Zoology, University of Genoa, Italy
    Cytometry 35:346-52. 1999
  4. ncbi request reprint Two-photon fluorescence excitation and related techniques in biological microscopy
    Alberto Diaspro
    Laboratory for Advanced Microscopy, Bioimaging and Spectroscopy LAMBS, MicroSCoBiO Research Center, University of Genoa, Italy
    Q Rev Biophys 38:97-166. 2005
  5. ncbi request reprint 3D localized photoactivation of pa-GFP in living cells using two-photon interactions
    Alberto Diaspro
    Dept of Physics, University of Genoa
    Conf Proc IEEE Eng Med Biol Soc 1:389-91. 2006
  6. ncbi request reprint Two-photon microscopy and spectroscopy based on a compact confocal scanning head
    A Diaspro
    University of Genoa, National Institute for the Physics of Matter INFM, Department of Physics, Via Dodecaneso 33, 16146 Genova, Italy
    J Biomed Opt 6:300-10. 2001
  7. ncbi request reprint Single-pinhole confocal imaging of sub-resolution sparse objects using experimental point spread function and image restoration
    A Diaspro
    INFM, Biophysical Section, Genoa Research Unit and Department of Physics, University of Genoa, Via Dodecaneso 33, 16146 Genova, Italy
    Microsc Res Tech 51:464-8. 2000
  8. ncbi request reprint Two-photon excitation of fluorescence for three-dimensional optical imaging of biological structures
    A Diaspro
    INFM and Department of Physics, University of Genoa, Italy
    J Photochem Photobiol B 55:1-8. 2000
  9. ncbi request reprint Microscopical characterization of nanocapsules templated on ionic crystals and biological cells toward biomedical applications
    Alberto Diaspro
    INFM, University of Genoa, 16146 Genoa, Italy
    IEEE Trans Nanobioscience 1:110-5. 2002
  10. ncbi request reprint Fluid phase and receptor-mediated endocytosis in Paramecium primaurelia by fluorescence confocal laser scanning microscopy
    P Ramoino
    P Ramoino 1R P Fronte DIP TE RIS, University of Genoa, Italy
    Eur Biophys J 30:305-12. 2001

Collaborators

Detail Information

Publications84

  1. pmc Multi-photon excitation microscopy
    Alberto Diaspro
    LAMBS MicroScoBio Research Center, Department of Physics, University of Genoa, Via Dodecaneso 33, 16146 Genova, Italy
    Biomed Eng Online 5:36. 2006
    ....
  2. pmc A new FRAP/FRAPa method for three-dimensional diffusion measurements based on multiphoton excitation microscopy
    Davide Mazza
    Laboratory for Advanced Microscopy, Bioimaging, and Spectroscopy MicroSCoBiO Research Center, Department of Physics, University of Genoa, Genoa, Italy
    Biophys J 95:3457-69. 2008
    ..Finally, we apply the method to photoactivatable green fluorescent protein diffusing freely in the nucleus of living NIH-3T3 mouse embryo fibroblasts...
  3. ncbi request reprint Cytofluorometry and fluorescence confocal laser scanning microscopy (CLSM) in the study of neutral lipid dynamics in Paramecium primaurelia mating types during cell line development
    P Ramoino
    Institute of Zoology, University of Genoa, Italy
    Cytometry 35:346-52. 1999
    ..The present study was done to determine whether cells that ingest more nutrients also have a larger amount of storage lipids...
  4. ncbi request reprint Two-photon fluorescence excitation and related techniques in biological microscopy
    Alberto Diaspro
    Laboratory for Advanced Microscopy, Bioimaging and Spectroscopy LAMBS, MicroSCoBiO Research Center, University of Genoa, Italy
    Q Rev Biophys 38:97-166. 2005
    ..As a conclusive comment, we believe that 2PE and related techniques can be considered as a mainstay of the modern biophysical research milieu and a bright perspective in optical microscopy...
  5. ncbi request reprint 3D localized photoactivation of pa-GFP in living cells using two-photon interactions
    Alberto Diaspro
    Dept of Physics, University of Genoa
    Conf Proc IEEE Eng Med Biol Soc 1:389-91. 2006
    ..This enables molecular trafficking studies at high signal to noise ratio...
  6. ncbi request reprint Two-photon microscopy and spectroscopy based on a compact confocal scanning head
    A Diaspro
    University of Genoa, National Institute for the Physics of Matter INFM, Department of Physics, Via Dodecaneso 33, 16146 Genova, Italy
    J Biomed Opt 6:300-10. 2001
    ....
  7. ncbi request reprint Single-pinhole confocal imaging of sub-resolution sparse objects using experimental point spread function and image restoration
    A Diaspro
    INFM, Biophysical Section, Genoa Research Unit and Department of Physics, University of Genoa, Via Dodecaneso 33, 16146 Genova, Italy
    Microsc Res Tech 51:464-8. 2000
    ..The key resides in the utilisation of the measured point spread function coupled to an appropriate image restoration approach, and, of course, in the stability of the confocal system being used...
  8. ncbi request reprint Two-photon excitation of fluorescence for three-dimensional optical imaging of biological structures
    A Diaspro
    INFM and Department of Physics, University of Genoa, Italy
    J Photochem Photobiol B 55:1-8. 2000
    ..The scanning head allows conventional and two-photon confocal imaging. Point spread function measurements are reported with examples of applications to the study of biological systems...
  9. ncbi request reprint Microscopical characterization of nanocapsules templated on ionic crystals and biological cells toward biomedical applications
    Alberto Diaspro
    INFM, University of Genoa, 16146 Genoa, Italy
    IEEE Trans Nanobioscience 1:110-5. 2002
    ..Nanocapsules are of biomedical interest because they can be used, for example, for the controlled release and targeting of drugs as well as for the protection of enzymes, proteins, and foreign cells...
  10. ncbi request reprint Fluid phase and receptor-mediated endocytosis in Paramecium primaurelia by fluorescence confocal laser scanning microscopy
    P Ramoino
    P Ramoino 1R P Fronte DIP TE RIS, University of Genoa, Italy
    Eur Biophys J 30:305-12. 2001
    ..Cytosol acidification and incubation in sucrose medium or in chlorpromazine showed that WGA is internalized via clathrin vesicles, whereas fluid phase endocytosis is a clathrin-independent process...
  11. ncbi request reprint Photoactivation of pa-GFP in 3D: optical tools for spatial confinement
    I Testa
    Department of Physics, University of Genoa, 16146, Genoa, Italy
    Eur Biophys J 37:1219-27. 2008
    ..These two photoactivation methods provide a suitable tool for many biological applications, combining subresolved surface and in-depth three-dimensionally confined investigations...
  12. ncbi request reprint Selective fluorescence recovery after bleaching of single E2GFP proteins induced by two-photon excitation
    G Chirico
    INFM and Department of Physics, University of Milano Bicocca, Piazza della Scienza 3, 20126 Milano, Italy
    Chemphyschem 6:328-35. 2005
    ..This GFP mutant therefore seems promising as an almost permanent chromophore for two-photon excitation (TPE) microscopy or for applications in single-molecule memory arrays...
  13. ncbi request reprint Improvement in volume estimation from confocal sections after image deconvolution
    F Difato
    INFM, LAMBS DIFI, University of Genoa, 16146, Italy
    Microsc Res Tech 64:151-5. 2004
    ..In order to compare the effective improvement in the quantitative image information analysis, we measured the volume of reference objects before and after image restoration, using the isotropic Fakir method...
  14. ncbi request reprint Morphology, mechanical properties and viability of encapsulated cells
    T Svaldo-Lanero
    Physics Department, University of Genoa, Via Dodecaneso 33, 16146 Genoa, Italy
    Ultramicroscopy 107:913-21. 2007
    ..CLSM analysis indicates that cells preserve their subcellular structure and duplication capability after encapsulation. By coupling AFM and CLSM data, a correlation between local stiffness and duplication rate was obtained...
  15. ncbi request reprint Mapping cholesteryl ester analogue uptake and intracellular flow in Paramecium by confocal fluorescence microscopy
    P Ramoino
    Department for the Study of the Territory and its Resources, University of Genoa, Corso Europa 26, I 16132 Genoa, Italy
    J Microsc 208:167-76. 2002
    ..In conclusion, this study points out that in P. primaurelia the cholesteryl ester enters the cell via food vacuoles and through the plasma membrane and, inside the cell, it alters cell functions...
  16. ncbi request reprint Multiphoton switching dynamics of single green fluorescent proteins
    G Chirico
    INFM and Department of Physics, University of Milano Bicocca, Piazza della Scienza 3, 20126 Milano, Italy
    Phys Rev E Stat Nonlin Soft Matter Phys 70:030901. 2004
    ..These results highlight a new photoconversion pathway for photochromic GFPs and can have significant applications in multi-photon confocal microscopy and in optical data-storage architectures...
  17. ncbi request reprint Unfolding time distribution of GFP by single molecule fluorescence spectroscopy
    G Chirico
    Department of Physics, University of Milano Bicocca, Piazza della Scienza 3, Milano, Italy
    Eur Biophys J 35:663-74. 2006
    ..5 M. A theoretical analysis of the distributions indicates that this feature is a fingerprint of the competition between unfolding and refolding processes when the protein is very far from the midpoint denaturant concentration...
  18. ncbi request reprint Encapsulated yeast cells inside Paramecium primaurelia: a model system for protection capability of polyelectrolyte shells
    S Krol
    INFM, Department of Physics, and DIPTERIS, University of Genoa, Via Dodecaneso 33, 16146 Genoa, Italy
    J Microsc 212:239-43. 2003
    ..Our preliminary results indicate the suitability of the introduced model as a test system of this permeability...
  19. ncbi request reprint Role of three-dimensional bleach distribution in confocal and two-photon fluorescence recovery after photobleaching experiments
    Davide Mazza
    Laboratory for Advanced Microscopy, Bioimaging, and Spectroscopy MicroSCoBiO Research Center, Department of Physics, University of Genoa, Italy
    Appl Opt 46:7401-11. 2007
    ..The results show that two-photon excitation more closely fits ideal bleaching patterns even in the event of fluorescence saturation, achieving estimations of diffusion coefficients within 20% accuracy of the correct value...
  20. pmc Tracking unfolding and refolding of single GFPmut2 molecules
    Fabio Cannone
    Department of Physics, University of Milan Bicocca, 20126 Milan, Italy
    Biophys J 89:2033-45. 2005
    ..Several unfolding/refolding cycles can be followed on the same molecules, indicating full reversibility of the process and, noticeably, a bias of denaturated molecules toward refolding in the original protonation state...
  21. doi request reprint Spatial control of pa-GFP photoactivation in living cells
    I Testa
    Department of Physics, University of Genoa, I 16146 Genoa, Italy
    J Microsc 230:48-60. 2008
    ..The presented results emphasize how photoactivation by non-linear excitation can provide a tool to increase contrast in widefield and confocal cellular imaging...
  22. ncbi request reprint Three-dimensional mapping of cholinergic molecules by confocal laser scanning microscopy in sea urchin larvae
    C Falugi
    Istituto di Anatomia Comparata, Universita di Genova, Genova, Italy
    Micron 33:233-9. 2002
    ..At metamorphosis, positivity to cholinergic molecules translocated to the juvenile, where a high frequency of mAChR- and ChAT-like positive cells was found...
  23. ncbi request reprint Electric field allowed molecular transitions for one and two photon excitation microscopy
    Partha Pratim Mondal
    International Center for Theoretical Physics, Trieste, Italy
    Eur Biophys J 37:1073-6. 2008
    ..An application of electric field may result in the excitation of poorly excitable biomolecules. This excitation technique may find applications in single- and multi-photon fluorescence microscopy, bioimaging and optical devices...
  24. doi request reprint Biophysical effects of the natural product euplotin C on the Paramecium membrane
    Paola Ramoino
    Dipartimento per lo Studio del Territorio e delle sue Risorse DIP TE RIS, Universita di Genova, Corso Europa 26, 16132, Genoa, Italy
    J Comp Physiol A Neuroethol Sens Neural Behav Physiol 195:1061-9. 2009
    ..Our data suggest that euplotin C enhances backward swimming in our unicellular model system by interacting with the ciliary Ca(2+) channel functions through the reduction of cell membrane fluidity...
  25. ncbi request reprint Localization of the cyclic ADP-ribose-dependent calcium signaling pathway in bovine rod outer segments
    Isabella Panfoli
    Department of Biology, University of Genoa, Genova, Italy
    Invest Ophthalmol Vis Sci 48:978-84. 2007
    ..In this study, the presence and functional role of the adenosine diphosphoribosyl (ADPR)-cyclase-cyclic ADP-ribose (cADPR) system in bovine retinal rod outer segments (ROS) was investigated...
  26. doi request reprint The GABAergic-like system in the cyprid of Balanus amphitrite (=Amphibalanus amphitrite) (Cirripedia, Crustacea)
    Lorenzo Gallus
    LIBiOM, DIBIO, University of Genoa, Viale Benedetto XV 5, I 16132, Genova, Italy
    Biofouling 26:155-65. 2010
    ..These results suggest a neurotransmitter/neuromodulatory role for GABA in thoracic muscle contraction and regulatory functions in compound eyes and antennules of B. amphitrite cyprids...
  27. ncbi request reprint Distribution of choline acetyltransferase immunoreactivity in the alimentary tract of the barnacle Balanus amphitrite (Cirripedia, Crustacea)
    Lorenzo Gallus
    LIBiOM, Dipartimento di Biologia, Universita di Genova, Viale Benedetto XV 5, I 16132 Genova, Italy
    Neurosci Lett 409:230-3. 2006
    ..These data seem to indicate the involvement of ACh in the gut functions of barnacle and suggest that the barnacle alimentary tract is more complex than previously thought and requires further study...
  28. ncbi request reprint Multilayer nanoencapsulation. New approach for immune protection of human pancreatic islets
    Silke Krol
    Department of Physics, University of Genoa, Via Dodecaneso 33, 16146 Genoa, Italy
    Nano Lett 6:1933-9. 2006
    ..In these first measurements the encapsulation with a multilayer nanocapsule was shown to be a possible alternative to the more space-consuming and random islet-trapping microencapsulation...
  29. doi request reprint Immunochemical or fluorescent labeling of vesicular subcellular fractions for microscopy imaging
    Isabella Panfoli
    Department of Biology, University of Genoa, Italy
    Microsc Res Tech 73:1086-90. 2010
    ..Therefore, the technique seems adequate to become an instrument to study the structure and the function of these and other subcellular fractions...
  30. doi request reprint NMDA R1 receptor distribution in the cyprid of Balanus amphitrite (=Amphibalanus amphitrite) (Cirripedia, Crustacea)
    Lorenzo Gallus
    DIBIO, University of Genoa, Viale Benedetto XV 5, I 16132 Genova, Italy
    Neurosci Lett 485:183-8. 2010
    ..The distribution pattern comparable to that of GABAergic molecules could indicate an interrelated agonistic/antagonistic role for these two systems, which could be considered as potential targets of combined antifouling strategies...
  31. doi request reprint Collagen containing microcapsules: smart containers for disease controlled therapy
    Laura Pastorino
    Department of Communication, Computer and System Sciences, University of Genova, Via all Opera Pia 13, 16145 Genova, Italy
    J Colloid Interface Sci 357:56-62. 2011
    ..The results demonstrated the possibility to fabricate a drug delivery system where the release of the drug is dependent on the biochemistry of the pathological state...
  32. doi request reprint Evidence for aerobic metabolism in retinal rod outer segment disks
    Isabella Panfoli
    DIBIO, University of Genoa, Viale Benedetto XV, 5, 16132 Genova, Italy
    Int J Biochem Cell Biol 41:2555-65. 2009
    ..These findings may provide important new insight into energy production in outer segments via aerobic metabolism and additional information about protein components in OS disk membranes...
  33. ncbi request reprint Functional expression of release-regulating glycine transporters GLYT1 on GABAergic neurons and GLYT2 on astrocytes in mouse spinal cord
    Luca Raiteri
    Department of Experimental Medicine, Pharmacology and Toxicology Section, University of Genoa, Italy
    Neurochem Int 52:103-12. 2008
    ....
  34. ncbi request reprint Nanostructured polyelectrolyte-based system as a toolbox for metal ions detection
    Emiliano Ronzitti
    LAMBS IFOM, MicroSCoBiO Research Center, Department of Physics DIFI, University of Genoa, Via Dodecaneso 33, Genoa, Italy
    J Fluoresc 18:375-81. 2008
    ..Results obtained will show the advantages and the potentialities of our system as a smart toolbox for metal ions detection...
  35. doi request reprint Proteomic analysis of the retinal rod outer segment disks
    Isabella Panfoli
    Department of Biology, University of Genoa, V le Benedetto XV 3, 16132 Genova, Italy
    J Proteome Res 7:2654-69. 2008
    ..This study represents a step toward a global physiological characterization of the disk proteome and provides information necessary for future studies on energy supply for phototransduction...
  36. doi request reprint Presence and distribution of FMRFamide-like immunoreactivity in the cyprid of the barnacle Balanus amphitrite (Cirripedia, Crustacea)
    Lorenzo Gallus
    LIBiOM, DIBIO, Universita di Genova, Viale Benedetto XV 5, I 16132 Genova, Italy
    Microsc Res Tech 72:101-9. 2009
    ..We hypothesize that these peptides act as integrators in the central nervous system, perform neuromuscular functions for thoracic limbs, trigger intestinal movements and, at the level of the frontal filament, play a neurosecretory role...
  37. ncbi request reprint Effect of the bioactive metabolite euplotin C on phagocytosis and fluid-phase endocytosis in the single-celled eukaryote Paramecium
    Paola Ramoino
    Dipartimento per lo Studio del Territorio e delle sue Risorse DIP TE RIS, Universita di Genova, Corso Europa 26, I 16132, Genova, Italy
    Aquat Toxicol 85:67-75. 2007
    ..The effect of euplotin C on the food vacuole formation and fluid-phase endocytosis is apparently mediated by a modification of microtubule network...
  38. ncbi request reprint Reduction of higher-order photobleaching in two-photon excitation microscopy
    Partha Pratim Mondal
    International Center for Theoretical Physics, Trieste, Italy
    Phys Rev E Stat Nonlin Soft Matter Phys 75:061904. 2007
    ..Hence the photobleaching due to one- and three-photon excitation can be eliminated and higher-order processes can be minimized owing to their small molecular cross section...
  39. ncbi request reprint The GABAergic-like system in the marine demosponge Chondrilla nucula
    Paola Ramoino
    Department for the Study of the Territory and its Resources, University of Genoa, 16132 Genova, Italy
    Microsc Res Tech 70:944-51. 2007
    ....
  40. ncbi request reprint Annular pupil filter under shot-noise condition for linear and non linear microscopy
    Emiliano Ronzitti
    LAMBS IFOM, MicroScoBio, Department of Physics, University of Genoa, Via Dodecaneso, 16145 Genoa, Italy
    Opt Express 17:6867-80. 2009
    ..We demonstrate high-frequency and resolution enhancement in the case of linear and non linear fluorescence microscope approach under shot-noise condition...
  41. ncbi request reprint Gamma-aminobutyric acid and related molecules in the sea fan Eunicella cavolini (Cnidaria: Octocorallia): a biochemical and immunohistochemical approach
    Laura Girosi
    Dipartimento Biologia, Universita di Genova, Genoa, Italy
    Cell Tissue Res 329:187-96. 2007
    ..The distribution of these different immunoreactivities has allowed us to hypothesize GABA involvement in nematocyst discharge, in body wall and enteric muscular contraction, in neuronal integration and in male gametocyte differentiation...
  42. ncbi request reprint Endocytosis of GABAB receptors modulates membrane excitability in the single-celled organism Paramecium
    Paola Ramoino
    Department for the Study of the Territory and its Resources DIP TE RIS, University of Genoa, Corso Europa 26, 16132 Genova, Italy
    J Cell Sci 119:2056-64. 2006
    ..These results suggest that GABAB receptor endocytosis into clathrin-coated and -uncoated vesicles represents an important mechanism in the modulation of swimming behavior in Paramecium...
  43. doi request reprint Metabotropic γ-aminobutyric acid (GABAB) receptors modulate feeding behavior in the calcisponge Leucandra aspera
    Paola Ramoino
    Dipartimento per lo studio del Territorio e delle sue Risorse DIPTERIS, Universita di Genova, Genova, Italy
    J Exp Zool A Ecol Genet Physiol 315:132-40. 2011
    ..These findings suggest that GABA receptors and their functional role are highly conservative traits in the animal kingdom prenervous system evolution...
  44. pmc Two-photon activation and excitation properties of PA-GFP in the 720-920-nm region
    Marc Schneider
    INFM Genoa, Department of Physics, University of Genoa, Italy
    Biophys J 89:1346-52. 2005
    ..Additionally, when comparing the conventional photoactivation at lambda = 405 nm with a two-photon one, a sharper and better controllable three-dimensional volume of activation is obtained...
  45. pmc Dynamics of green fluorescent protein mutant2 in solution, on spin-coated glasses, and encapsulated in wet silica gels
    Giuseppe Chirico
    Department of Physics, University of Milan Bicocca, 20126 Milan, Italy
    Protein Sci 11:1152-61. 2002
    ..Overall, these data indicate that silica gels represent an ideal matrix for following biologically relevant events at a single molecule level...
  46. ncbi request reprint GABAA receptor subunits identified in Paramecium by immunofluorescence confocal microscopy
    Paola Ramoino
    Department for the Study of the Territory and its Resources DIP TE RIS, University of Genoa, Corso Europa 26, I 16132 Genoa, Italy
    FEMS Microbiol Lett 238:449-53. 2004
    ..The intensity of labelling of the alpha(6)-, beta(2/3)- and gamma(2)-subunits was more intense than the alpha(1)-, epsilon-, and theta-subunits, suggesting that the former are present in higher concentrations than the latter...
  47. ncbi request reprint Encapsulated living cells on microstructured surfaces
    Silke Krol
    INFM, DIFI, University of Genoa, Genoa, Italy, and Department of Interfaces, MPI of Colloids and Interfaces, Golm, Germany
    Langmuir 21:705-9. 2005
    ..In accordance with prior work, we find that coating does not kill the cells and coated GFP-expressing cells still function after immobilization, which we checked by fluorescence microscopy...
  48. ncbi request reprint Nanocapsules: coating for living cells
    Silke Krol
    National Institute for the Physics of Matter INFM, Department of Physics, University of Genoa, Genoa 33 16146, Italy
    IEEE Trans Nanobioscience 3:32-8. 2004
    ..The capsules exhibit interesting properties as valuable tool in science and a promising candidate for application in the field of medicine...
  49. ncbi request reprint GABAB receptor intracellular trafficking after internalization in Paramecium
    Paola Ramoino
    Department for the Study of Territory and its Resources DIP TE RIS, University of Genoa, 16132 Genoa, Italy
    Microsc Res Tech 68:290-5. 2005
    ..GABAB vesicle structures also colocalize with TGN38-IR and rab11-IR. TGN38 and rab11 are proteins found in association with post-Golgi and recycling endosomes, respectively...
  50. ncbi request reprint Swimming behavior regulation by GABAB receptors in Paramecium
    Paola Ramoino
    Department for the Study of Territory and its Resources DIP TE RIS, University of Genoa, Corso Europa 26, 16132 Genova, Italy
    Exp Cell Res 291:398-405. 2003
    ..Finally, synthesis and release of GABA in the environment by Paramecium have been demonstrated by HPLC. Possible correlations between GABA(B) receptor activation and the regulation of intracellular Ca(2+) levels are discussed...
  51. ncbi request reprint Glia re-sealed particles freshly prepared from adult rat brain are competent for exocytotic release of glutamate
    Sara Stigliani
    Pharmacology and Toxicology Section, Department of Experimental Medicine, University of Genoa, Genoa, Italy
    J Neurochem 96:656-68. 2006
    ..It is concluded that gliosomes purified from adult brain contain glutamate-accumulating vesicles and can release the amino acid by a process resembling neuronal exocytosis...
  52. doi request reprint Gamma-amino butyric acid (GABA) release in the ciliated protozoon Paramecium occurs by neuronal-like exocytosis
    P Ramoino
    Department for the Study of Territory and its Resources DIP TE RIS, University of Genoa, Corso Europa 26, Genoa, Italy
    J Exp Biol 213:1251-8. 2010
    ....
  53. ncbi request reprint Fuzzy logic and maximum a posteriori-based image restoration for confocal microscopy
    Giuseppe Vicidomini
    LAMBS MicroSCoBiO, DISI, University of Genoa, Genoa, Italy
    Opt Lett 31:3582-4. 2006
    ..Unlike other potentials, the fuzzy potential distinguishes intensity variation due to genuine edges and noise. The proposed approach has generated artifact-free restored confocal microscopy images...
  54. doi request reprint Live imaging of mammalian retina: rod outer segments are stained by conventional mitochondrial dyes
    Paolo Bianchini
    University of Genoa, Laboratory for Advanced, Microscopy, Bioimaging, and Spectroscopy LAMBS, MicroSCoBiO Research Center, Department of Physics, Via Dodecaneso 33, 16146, Genoa, Italy
    J Biomed Opt 13:054017. 2008
    ..This finding may shed light on previous reports of the existence of a proton potential across the disk membranes and on the mechanism of the adenosine tri-phosphate (ATP) supply for phototransduction, which still requires investigation...
  55. ncbi request reprint Activation of gamma-aminobutyric acid GAT-1 transporters on glutamatergic terminals of mouse spinal cord mediates glutamate release through anion channels and by transporter reversal
    Luca Raiteri
    Department of Experimental Medicine, Pharmacology and Toxicology Section, University of Genoa, Genoa, Italy
    J Neurosci Res 80:424-33. 2005
    ..Activation of these GABA transporters elicits release of glutamate partially by reversal of glutamate transporters present on glutamatergic terminals and largely through anion channels...
  56. ncbi request reprint Voltage regulation of single green fluorescent protein mutants
    Fabio Cannone
    Department of Physics, Labs, University of Milano Bicocca, Piazza della Scienza 3, 20126 Milano, Italy
    Biophys Chem 125:368-74. 2007
    ..These findings suggest that fluorescence blinking itself might be employed as a reporter signal in nano-bio-technology applications...
  57. doi request reprint A novel approach for correlative light electron microscopy analysis
    Giuseppe Vicidomini
    MicroSCoBiO Research Center, University of Genoa, 16146, Genoa, Italy
    Microsc Res Tech 73:215-24. 2010
    ..This method has been successfully validated for the correlative analysis of Russel Bodies subcellular compartments...
  58. ncbi request reprint Three-dimensional microscopy migrates to the web with "PowerUp Your Microscope"
    Paola Bonetto
    Department of Computer Sciences DISI, National Institute for the Physics of Matter INFM, University of Genoa, Italy
    Microsc Res Tech 64:196-203. 2004
    ..This project constitutes a real and effective migration to the Web, extending computational approaches to image restoration to the whole microscopy user community, regardless of their background...
  59. ncbi request reprint Interaction of polyelectrolytes and their composites with living cells
    Munish Chanana
    CNR INFM, Department of Physics, University of Genoa, 16146 Genoa, Italy
    Nano Lett 5:2605-12. 2005
    ..We found that toxicity is influenced by all these factors and cannot be tested easily in a model...
  60. ncbi request reprint Notes on theory and experimental conditions behind two-photon excitation microscopy
    Alessandro Esposito
    Department of Physics, University of Genoa, 16146 Genoa, Italy
    Microsc Res Tech 63:12-7. 2004
    ..The optical sectioning effect is demonstrated as well as the distribution of excitation and of fluorescence emission...
  61. doi request reprint Structural stability of green fluorescent proteins entrapped in polyelectrolyte nanocapsules
    Silke Krol
    LAMBS, MicroScoBiO Research Center and Department of Physics, University of Genoa, Via Dodecaneso 33, 16146 Genoa, Italy
    J Biophotonics 1:310-9. 2008
    ..This suggestion is supported by Fluorescence Polarization measurements on GFPmut2 proteins bound to the NC layers...
  62. ncbi request reprint Introduction: A nanoworld under the microscope--from cell trafficking to molecular machines
    Alberto Diaspro
    MicroScoBio LAMBS INFM IFOM, Department of Physics, University of Genoa, Genova, Italy
    Microsc Res Tech 65:167-8. 2004
  63. ncbi request reprint Rapid dissemination of two-photon excitation microscopy prompts new applications
    Alberto Diaspro
    Department of Physics, University of Genoa Genova, Italy
    Microsc Res Tech 63:1-2. 2004
  64. doi request reprint Three-dimensional (3D) backward and forward second harmonic generation (SHG) microscopy of biological tissues
    Paolo Bianchini
    LAMBS, MicroScoBiO Research Center and Department of Physics, University of Genoa, Via Dodecaneso 33, 16146 Genoa, Italy
    J Biophotonics 1:443-50. 2008
    ..We discuss here some key elements related to the backward and forward SHG signal in terms of acquisition architecture and related microscopic imaging...
  65. ncbi request reprint Shine on ... proteins
    Alberto Diaspro
    LAMBS MicroScoBio and IFOM Research Centre, Department of Physics, University of Genoa, Genoa, Italy
    Microsc Res Tech 69:149-51. 2006
  66. pmc Two-photon thermal bleaching of single fluorescent molecules
    Giuseppe Chirico
    Istituto Nazionale per la Fisica della Materia, U d R Milano Bicocca, Via Cozzi 52, Italy
    Biophys J 84:588-98. 2003
    ..These observations are interpreted as thermal bleaching where the temperature increase is induced by the two-photon absorption of the single dyes as confirmed also by numerical simulations...
  67. ncbi request reprint Single molecule spectroscopic characterization of GFP-MUT2 mutant for two-photon microscopy applications
    Fabio Cannone
    Dipartimento di Fisica, Universita di Milano Bicocca, Milano, Italy
    Microsc Res Tech 65:186-93. 2004
    ..Our results indicate that GFPmut2 can be employed as a long-lived reporter of biological processes...
  68. ncbi request reprint Dimethyl-pepep: a DNA probe in two-photon excitation cellular imaging
    Alessandro Abbotto
    Dipartimento di Scienza dei Materiali and INSTM, Universita di Milano Bicocca, Italy
    Biophys Chem 114:35-41. 2005
    ..Preliminary tests have shown that the dye cellular toxicity is negligible...
  69. ncbi request reprint Two-photon interactions at single fluorescent molecule level
    Fabio Cannone
    National Institute for the Physics of Matter, Laboratory for Advanced, Microscopy, Bioimaging, and Spectroscopy, Genoa, Italy
    J Biomed Opt 8:391-5. 2003
    ....
  70. ncbi request reprint FMRFamide-like immunoreactivity in the sea-fan Eunicella cavolini (Cnidaria: Octocorallia)
    Laura Girosi
    Dipartimento delle Scienze Biologiche Sez Zoologia, Universita di Napoli Federico II, Italy
    Cell Tissue Res 320:331-6. 2005
    ..These results improve our knowledge of the gorgonian nervous system and demonstrate that most of the immunoreactive cells belong to neural elements...
  71. ncbi request reprint A role for GABAA receptors in the modulation of Paramecium swimming behavior
    Giovanna Bucci
    Institute of Biophysics, National Research Council, Via De Marini, 6, I 16149 Genoa, Italy
    Neurosci Lett 386:179-83. 2005
    ..This inhibition suggests that an inflow of calcium ions through L-type channels mediates the muscimol-induced swimming behavior...
  72. ncbi request reprint Confocal laser scanning microscopy of retinal rod outer segment intact disks: new labeling technique
    Silvia Ravera
    J Biomed Opt 12:050501. 2007
    ..In fact, even though much is known about the rod outer segment photoresponse, some unanswered questions remain, particularly about ATP supply, light adaptation, and morphogenesis...
  73. ncbi request reprint Nanocapsules: a European Community interdisciplinary network in the nanobiosciences
    Alberto Diaspro
    IEEE Trans Nanobioscience 3:1-2. 2004
  74. ncbi request reprint Amyloid precursor protein and Presenilin1 interact with the adaptor GRB2 and modulate ERK 1,2 signaling
    Mario Nizzari
    Dipartimento di Oncologia, Biologia e Genetica, Universita di Genova, Viale Benedetto XV, 2, 16132 Genova, Italy
    J Biol Chem 282:13833-44. 2007
    ..These data suggest that both APP and presenilin1 can be part of a common signaling pathway that regulates ERK1,2 and the cell cycle...
  75. ncbi request reprint Neuronal apoptosis is accompanied by amyloid beta-protein accumulation in the endoplasmic reticulum
    Roberta Borghi
    Institute of Human Anatomy, Department of Experimental Medicine, University of Genova, Italy
    J Alzheimers Dis 4:31-7. 2002
    ..Moreover, our data indicate that still unidentified cellular mechanisms, other than caspases activation, are responsible of the specific alteration of A betaPP processing during PCD...
  76. ncbi request reprint Amyloid precursor protein and Presenilin 1 interaction studied by FRET in human H4 cells
    Mario Nizzari
    Pharmacology, Department of Oncology, Biology and Genetics, University of Genova, Genova, Italy
    Ann N Y Acad Sci 1096:249-57. 2007
    ..FRET data suggest a close interaction between APP and PS1 in subcellular compartments and at the centrosome of H4 cells. Using this approach we show that APP and PS1 are closely associated in the centrosomes of the H4 cell...
  77. ncbi request reprint Unraveling the organization of the internal nuclear matrix: RNA-dependent anchoring of NuMA to a lamin scaffold
    Paola Barboro
    Istituto Nazionale per la Ricerca sul Cancro, I 16132, Genova, Italy
    Exp Cell Res 279:202-18. 2002
    ....
  78. pmc SOD2 functions downstream of Sch9 to extend longevity in yeast
    Paola Fabrizio
    Andrus Gerontology Center and Department of Biological Sciences, University of Southern California, Los Angeles, California 90089 0191, USA
    Genetics 163:35-46. 2003
    ..cerevisiae to superoxide dismutases and suggest that the induction of other stress-resistance genes regulated by Msn2/4 and Rim15 is required for maximum longevity extension...
  79. doi request reprint Visible fluorescent proteins
    Ammasi Periasamy
    J Biomed Opt 13:031201. 2008
  80. ncbi request reprint Multiphoton microscopy
    Ammasi Periasamy
    J Biomed Opt 8:327-8. 2003
  81. doi request reprint Endocytic trafficking of Rac is required for the spatial restriction of signaling in cell migration
    Andrea Palamidessi
    IFOM, Fondazione Istituto FIRC di Oncologia Molecolare, Via Adamello 16, 20139 Milan, Italy
    Cell 134:135-47. 2008
    ....
  82. ncbi request reprint Evidence for ciliary pigment localization in colored ciliates and implications for their photosensory transduction chain: a confocal microscopy study
    Giuliano Colombetti
    CNR Institute of Biophysics, Italy
    Microsc Res Tech 70:1028-33. 2007
    ..In an ordered scale from lower to greater efficiency, we have S. coeruleus, B. japonicum blue, B. japonicum red, and F. salina. The possible implications of our findings for the process of photosensory transduction are discussed...
  83. pmc Superoxide is a mediator of an altruistic aging program in Saccharomyces cerevisiae
    Paola Fabrizio
    Andrus Gerontology Center and Dept of Biological Sciences, University of Southern California, 3715 McClintock Avenue, Los Angeles, CA 90089 0191, USA
    J Cell Biol 166:1055-67. 2004
    ..The role of similar pathways in the regulation of longevity in organisms ranging from yeast to mice raises the possibility that mammals may also undergo programmed aging...
  84. ncbi request reprint Involvement of p53 in specific anti-neuroectodermal tumor activity of aloe-emodin
    Teresa Pecere
    Department of Histology, Microbiology and Medical Biotechnology, University of Padova, Padua, Italy
    Int J Cancer 106:836-47. 2003
    ..Due to its high accumulation in neuroectodermal tumor cells AE could also kill tumor cells harboring p53 mutant genes. This property would further contribute to AE specific anti-tumor activity and might be exploitable in the clinic...