Isabella Dalle-Donne

Summary

Affiliation: University of Milan
Country: Italy

Publications

  1. ncbi Blood glutathione disulfide: in vivo factor or in vitro artifact?
    Ranieri Rossi
    Department of Neuroscience, Pharmacology Unit, Via A Moro 4, University of Siena, 53100 Siena, Italy
    Clin Chem 48:742-53. 2002
  2. ncbi Protein carbonyl groups as biomarkers of oxidative stress
    Isabella Dalle-Donne
    Department of Biology, University of Milan, Italy
    Clin Chim Acta 329:23-38. 2003
  3. ncbi Protein carbonylation, cellular dysfunction, and disease progression
    Isabella Dalle-Donne
    Department of Biology, University of Milan, Italy
    J Cell Mol Med 10:389-406. 2006
  4. ncbi Intervention strategies to inhibit protein carbonylation by lipoxidation-derived reactive carbonyls
    Giancarlo Aldini
    Institute of Pharmaceutical and Toxicological Chemistry, Faculty of Pharmacy, University of Milan, Viale Abruzzi 42, I 20131, Milan, Italy
    Med Res Rev 27:817-68. 2007
  5. pmc Red blood cells protect albumin from cigarette smoke-induced oxidation
    Graziano Colombo
    Department of Biology, Universita degli Studi di Milano, Milan, Italy
    PLoS ONE 7:e29930. 2012
  6. ncbi S-glutathionylation in human platelets by a thiol-disulfide exchange-independent mechanism
    Isabella Dalle-Donne
    Department of Biology, University of Milan, Via Celoria 26, I 20133 Milan, Italy
    Free Radic Biol Med 38:1501-10. 2005
  7. ncbi Biomarkers of oxidative damage in human disease
    Isabella Dalle-Donne
    Department of Biology, University of Milan, Milan, Italy
    Clin Chem 52:601-23. 2006
  8. ncbi Is there an answer?
    Isabella Dalle-Donne
    Department of Biology, University of Milan, Milan, Italy
    IUBMB Life 57:189-92. 2005
  9. ncbi Actin Cys374 as a nucleophilic target of alpha,beta-unsaturated aldehydes
    Isabella Dalle-Donne
    Department of Biology, University of Milan, Via Celoria 26, I 20133 Milan, Italy
    Free Radic Biol Med 42:583-98. 2007
  10. ncbi S-glutathionylation in protein redox regulation
    Isabella Dalle-Donne
    Department of Biology, University of Milan, I 20133 Milan, Italy
    Free Radic Biol Med 43:883-98. 2007

Detail Information

Publications43

  1. ncbi Blood glutathione disulfide: in vivo factor or in vitro artifact?
    Ranieri Rossi
    Department of Neuroscience, Pharmacology Unit, Via A Moro 4, University of Siena, 53100 Siena, Italy
    Clin Chem 48:742-53. 2002
    ..We have identified and investigated possible pitfalls in measurement of both GSH and GSSG...
  2. ncbi Protein carbonyl groups as biomarkers of oxidative stress
    Isabella Dalle-Donne
    Department of Biology, University of Milan, Italy
    Clin Chim Acta 329:23-38. 2003
    ..At present, the measurement of protein CO groups after their derivatisation with DNPH is the most widely utilized measure of protein oxidation...
  3. ncbi Protein carbonylation, cellular dysfunction, and disease progression
    Isabella Dalle-Donne
    Department of Biology, University of Milan, Italy
    J Cell Mol Med 10:389-406. 2006
    ....
  4. ncbi Intervention strategies to inhibit protein carbonylation by lipoxidation-derived reactive carbonyls
    Giancarlo Aldini
    Institute of Pharmaceutical and Toxicological Chemistry, Faculty of Pharmacy, University of Milan, Viale Abruzzi 42, I 20131, Milan, Italy
    Med Res Rev 27:817-68. 2007
    ..Inhibition of ALE formation, based on carbonyl-sequestering agents, seems to be the most promising pharmacological tool and is reviewed in detail...
  5. pmc Red blood cells protect albumin from cigarette smoke-induced oxidation
    Graziano Colombo
    Department of Biology, Universita degli Studi di Milano, Milan, Italy
    PLoS ONE 7:e29930. 2012
    ..Differently, human erythrocytes resulted to be protective towards CSE-induced oxidation (carbonylation and thiol oxidation) of both HSA and total human plasma proteins...
  6. ncbi S-glutathionylation in human platelets by a thiol-disulfide exchange-independent mechanism
    Isabella Dalle-Donne
    Department of Biology, University of Milan, Via Celoria 26, I 20133 Milan, Italy
    Free Radic Biol Med 38:1501-10. 2005
    ....
  7. ncbi Biomarkers of oxidative damage in human disease
    Isabella Dalle-Donne
    Department of Biology, University of Milan, Milan, Italy
    Clin Chem 52:601-23. 2006
    ..Here we discuss some of the more commonly used biomarkers of oxidative/nitrosative damage and include selected examples of human studies...
  8. ncbi Is there an answer?
    Isabella Dalle-Donne
    Department of Biology, University of Milan, Milan, Italy
    IUBMB Life 57:189-92. 2005
  9. ncbi Actin Cys374 as a nucleophilic target of alpha,beta-unsaturated aldehydes
    Isabella Dalle-Donne
    Department of Biology, University of Milan, Via Celoria 26, I 20133 Milan, Italy
    Free Radic Biol Med 42:583-98. 2007
    ....
  10. ncbi S-glutathionylation in protein redox regulation
    Isabella Dalle-Donne
    Department of Biology, University of Milan, I 20133 Milan, Italy
    Free Radic Biol Med 43:883-98. 2007
    ..In this review, we describe recent breakthroughs in our understanding of the potential role of protein S-glutathionylation in the redox regulation of signal transduction...
  11. ncbi Molecular mechanisms and potential clinical significance of S-glutathionylation
    Isabella Dalle-Donne
    Department of Biology, University of Milan, Milan, Italy
    Antioxid Redox Signal 10:445-73. 2008
    ..Recently, specific PSSG have been identified in the inferior parietal lobule in Alzheimer's disease. However, much investigation is needed to clarify the actual involvement of protein S-glutathionylation in many human diseases...
  12. ncbi Proteins as biomarkers of oxidative/nitrosative stress in diseases: the contribution of redox proteomics
    Isabella Dalle-Donne
    Department of Biology, University of Milan, Via Celoria 26, I 20133, Milan, Italy
    Mass Spectrom Rev 24:55-99. 2005
    ..Indeed, identification and characterization of oxidatively/nitrosatively modified proteins in human diseases has just begun...
  13. doi Protein S-glutathionylation: a regulatory device from bacteria to humans
    Isabella Dalle-Donne
    Department of Biology, University of Milan, Via Celoria 26, I 20133, Milan, Italy
    Trends Biochem Sci 34:85-96. 2009
    ..Moreover, recent studies have identified S-glutathionylation as a redox signalling mechanism in plants...
  14. doi Protein carbonylation: 2,4-dinitrophenylhydrazine reacts with both aldehydes/ketones and sulfenic acids
    Isabella Dalle-Donne
    Department of Biology, University of Milan, Italy
    Free Radic Biol Med 46:1411-9. 2009
    ..beta-Mercaptoethanol prevents the formation of the DNPH derivative because it reacts with the oxidized Cys residue, forming the corresponding disulfide...
  15. ncbi Actin S-glutathionylation: evidence against a thiol-disulphide exchange mechanism
    Isabella Dalle-Donne
    Department of Biology, University of Milan, Milan, Italy
    Free Radic Biol Med 35:1185-93. 2003
    ....
  16. doi S-glutathiolation in life and death decisions of the cell
    Isabella Dalle-Donne
    Department of Biology, Universita degli Studi di Milano, Milan, Italy
    Free Radic Res 45:3-15. 2011
    ..However, the effective relevance of protein S-glutathiolation and the precise molecular targets in apoptotic signalling remain unresolved and a key challenge for future research...
  17. ncbi Familial amyotrophic lateral sclerosis (FALS): Emerging hints from redox proteomics. Highlight commentary on: "Redox proteomics analysis of oxidatively modified proteins in G93A-SOD1 transgenic mice--a model of familial amyotrophic lateral sclerosis&
    Isabella Dalle-Donne
    Department of Biology, University of Milan, Via Celoria 26, I 20133 Milan, Italy
    Free Radic Biol Med 43:157-9. 2007
  18. doi Water-Soluble alpha,beta-unsaturated aldehydes of cigarette smoke induce carbonylation of human serum albumin
    Graziano Colombo
    Dipartimento di Biologia, Universita degli Studi di Milano, Milan, Italy
    Antioxid Redox Signal 12:349-64. 2010
    ..Carbonylation of HSA associated with cigarette smoking might result in modifications of its antioxidant properties and transport functions of both endogenous and exogenous compounds...
  19. doi Cellular redox potential and hemoglobin S-glutathionylation in human and rat erythrocytes: A comparative study
    Graziano Colombo
    Department of Biology, Universita degli Studi di Milano, Milan, Italy
    Blood Cells Mol Dis 44:133-9. 2010
    ....
  20. doi Oxidative damage in human gingival fibroblasts exposed to cigarette smoke
    Graziano Colombo
    Department of Biology, Universita degli Studi di Milano, I 20133 Milan, Italy
    Free Radic Biol Med 52:1584-96. 2012
    ..GSH depletion could be a mechanism for cigarette smoke-induced cytotoxicity and could be correlated with the reduced reparative and regenerative activity of gingival and periodontal tissues previously reported in smokers...
  21. ncbi Redox albuminomics: oxidized albumin in human diseases
    Graziano Colombo
    Department of Life Sciences, University of Milan, Milan, Italy
    Antioxid Redox Signal 17:1515-27. 2012
    ..As albumin is the predominant protein in most body fluids, its Cys34 represents the largest fraction of free thiols within body fluids...
  22. ncbi Protein carbonylation in human diseases
    Isabella Dalle-Donne
    Department of Biology, University of Milan, Via Celoria 26, I 20133 Milan, Italy
    Trends Mol Med 9:169-76. 2003
    ....
  23. doi Cigarette smoke induces alterations in the drug-binding properties of human serum albumin
    Marco Clerici
    Department of Biosciences, Universita degli Studi di Milano, Milan, Italy Department of Biomedical Sciences for Health, Universita degli Studi di Milano, Milan, Italy
    Blood Cells Mol Dis 52:166-74. 2014
    ..These findings suggest that cigarette smoke-induced modifications of HSA may affect the binding, transport and bioavailability of specific ligands in smokers. ..
  24. ncbi Identification of actin as a 15-deoxy-Delta12,14-prostaglandin J2 target in neuroblastoma cells: mass spectrometric, computational, and functional approaches to investigate the effect on cytoskeletal derangement
    Giancarlo Aldini
    Istituto di Chimica Farmaceutica e Tossicologica Pietro Pratesi, Faculty of Pharmacy, University of Milan, I 20131 Milan, Italy
    Biochemistry 46:2707-18. 2007
    ....
  25. ncbi Covalent modification of actin by 4-hydroxy-trans-2-nonenal (HNE): LC-ESI-MS/MS evidence for Cys374 Michael adduction
    Giancarlo Aldini
    Istituto Chimico Farmaceutico Tossicologico, University of Milan, Viale Abruzzi 42, I 20131 Milan, Italy
    J Mass Spectrom 40:946-54. 2005
    ..Computational studies showed that the reactivity of Cys374 residue is due to a significant accessible surface and substantial thiol acidity due to the particular microenvironment surrounding Cys374...
  26. ncbi Lipoxidation-derived reactive carbonyl species as potential drug targets in preventing protein carbonylation and related cellular dysfunction
    Giancarlo Aldini
    Institute of Pharmaceutical and Toxicological Chemistry Pietro Pratesi, Faculty of Pharmacy, University of Milan, Viale Abruzzi 42, 20131, Milan, Italy
    ChemMedChem 1:1045-58. 2006
  27. doi Pathophysiology of tobacco smoke exposure: Recent insights from comparative and redox proteomics
    Graziano Colombo
    Department of Biosciences, Universita degli Studi di Milano, Milan, Italy
    Mass Spectrom Rev 33:183-218. 2014
    ..2013 Wiley Periodicals, Inc. Mass Spec Rev 33: 183-218, 2014. ..
  28. pmc Sex-related effects of reproduction on biomarkers of oxidative damage in free-living barn swallows (Hirundo rustica)
    Diego Rubolini
    Dipartimento di Bioscienze, Universita degli Studi di Milano, Milano, Italy
    PLoS ONE 7:e48955. 2012
    ..Moreover, the higher protein OD levels observed among females compared to males suggest that egg laying entails oxidative costs, which might negatively affect female residual reproductive value...
  29. doi The potential of resveratrol against human gliomas
    Nicoletta Gagliano
    Department of Human Morphology and Biomedical Sciences Citta Studi, Universita di Milano, School of Medicine, Extracellular Matrix Laboratory, Via Fratelli Cervi 93, 20090 Segrate, Milan, Italy
    Anticancer Drugs 21:140-50. 2010
    ..This study discusses current knowledge with regard to the effects of resveratrol in relation to its potential as a chemopreventive and/or chemotherapeutic molecule against human gliomas...
  30. doi Pancreatic cancer cells retain the epithelial-related phenotype and modify mitotic spindle microtubules after the administration of ukrain in vitro
    Nicoletta Gagliano
    Department of Biomedical Sciences for Health, EML Extracellular Matrix Laboratory, Universita degli Studi di Milano, Milan, Italy
    Anticancer Drugs 23:935-46. 2012
    ..The differentiated epithelial phenotype of HPAF-II, HPAC, and PL45 cell lines concomitant with a highly invasive potential suggests that further experiments will be necessary to definitively clarify the role of EMT in PDAC progression...
  31. ncbi Protein glutathionylation in erythrocytes
    Daniela Giustarini
    Department of Neuroscience, Pharmacology Section, University of Siena, Via A Moro 4, 53100 Siena, Italy
    Clin Chem 49:327-30. 2003
  32. ncbi An improved HPLC measurement for GSH and GSSG in human blood
    Daniela Giustarini
    Department of Neuroscience, University of Siena, Siena, Italy
    Free Radic Biol Med 35:1365-72. 2003
    ..e., 1.4 pmoles) and a high reproducibility with a within-run imprecision of less than 2%. It could have a wide application as it is simple, virtually artifact-free, and not time-consuming, especially for large-scale screening studies...
  33. ncbi S-glutathionylation: from redox regulation of protein functions to human diseases
    Daniela Giustarini
    Department of Neuroscience, University of Siena, Siena, Italy
    J Cell Mol Med 8:201-12. 2004
    ..S-glutathionylated proteins are now actively investigated with reference to problems of biological interest and as possible biomarkers of human diseases associated with oxidative/nitrosative stress...
  34. ncbi Protein S-glutathionylation and platelet anti-aggregating activity of disulfiram
    Ranieri Rossi
    Department of Neuroscience, University of Siena, I 53100 Siena, Italy
    Biochem Pharmacol 72:608-15. 2006
    ....
  35. ncbi S-nitrosation versus S-glutathionylation of protein sulfhydryl groups by S-nitrosoglutathione
    Daniela Giustarini
    Department of Neuroscience, Pharmacology Unit, University of Siena, Siena, Italy
    Antioxid Redox Signal 7:930-9. 2005
    ..The susceptibility of the modified proteins to denitrosation and deglutathionylation by reduced glutathione was also investigated...
  36. ncbi Age-related influence on thiol, disulfide, and protein-mixed disulfide levels in human plasma
    Daniela Giustarini
    Department of Neuroscience, Pharmacology Unit, University of Siena, Via A Moro 4, I 53100, Siena, Italy
    J Gerontol A Biol Sci Med Sci 61:1030-8. 2006
    ..The occurrence of two distinct regulatory systems for plasmatic pools of glutathione/cysteinylglycine on the one hand and cysteine/homocysteine on the other hand is hypothesized...
  37. ncbi Oxidized forms of glutathione in peripheral blood as biomarkers of oxidative stress
    Ranieri Rossi
    Department of Neuroscience, Pharmacology Section, University of Siena, Siena, Italy
    Clin Chem 52:1406-14. 2006
    ..We evaluated the importance of correct sample handling during the preanalytical phase for GSH, GSSG, and PSSG measurement...
  38. ncbi Metabolism of oxidants by blood from different mouse strains
    Daniela Giustarini
    Department of Neurosciences, Pharmacology Unit, University of Siena, Via A Moro 4, 53100 Siena, Italy
    Biochem Pharmacol 71:1753-64. 2006
    ..Because of the widely different reactivity of haemoglobin cysteinyl residues, the mouse strains examined are an interesting but complicated model in which to study the pharmacological and toxicological action of some drugs...
  39. ncbi Red blood cells as a physiological source of glutathione for extracellular fluids
    Daniela Giustarini
    Department of Evolutionary Biology, Laboratory of Pharmacology and Toxicology, University of Siena, Via A Moro 4, 53100 Siena, Italy
    Blood Cells Mol Dis 40:174-9. 2008
    ..These results suggest that erythrocytes can contribute significantly to the extracellular pool of glutathione (GSH), thus cooperating with liver and other tissues to the dynamics of inter-organ GSH metabolism...
  40. ncbi Membrane skeletal protein S-glutathionylation and hemolysis in human red blood cells
    Ranieri Rossi
    Department of Neuroscience, University of Siena, I 53100 Siena, Italy
    Blood Cells Mol Dis 37:180-7. 2006
    ..2 and spectrin could contribute to regulate RBC membrane stability...
  41. ncbi Nitric oxide and S-nitrosothiols in human blood
    Daniela Giustarini
    Pharmacology Section, Department of Neuroscience, University of Siena, Via A Moro 4, Italy
    Clin Chim Acta 330:85-98. 2003
    ..Particular attention is addressed to conflicting results and to discrepancies in the methodologies and models utilized. The numerous unanswered questions concerning the role of RSNOs in the control of vascular tone are discussed...
  42. ncbi Detection of S-nitrosothiols in biological fluids: a comparison among the most widely applied methodologies
    Daniela Giustarini
    Department of Neuroscience, Pharmacology Section, Via A Moro 4, University of Siena, 53100 Siena, Italy
    J Chromatogr B Analyt Technol Biomed Life Sci 851:124-39. 2007
    ..Furthermore, some possible methodological drawbacks, responsible for the highlighted discrepancies, are evidenced...
  43. ncbi Interference of plasmatic reduced glutathione and hemolysis on glutathione disulfide levels in human blood
    Daniela Giustarini
    Department of Neuroscience, Pharmacology Section, University of Siena, Via A Moro 4, I 53100, Siena, Italy
    Free Radic Res 38:1101-6. 2004
    ..Our results show that, before performing blood GSSG determination, thiols have to be rapidly blocked, to avoid possible pitfalls in GSSG measurement, in particular when hemolysis is present...