Stefano Ciurli

Summary

Affiliation: University of Bologna
Country: Italy

Publications

  1. ncbi Molecular characterization of Bacillus pasteurii UreE, a metal-binding chaperone for the assembly of the urease active site
    Stefano Ciurli
    Department of Agro Environmental Science and Technology, University of Bologna, Via Filippo Re 8, Italy
    J Biol Inorg Chem 7:623-31. 2002
  2. ncbi UreG, a chaperone in the urease assembly process, is an intrinsically unstructured GTPase that specifically binds Zn2+
    Barbara Zambelli
    Laboratory of Bioinorganic Chemistry, Department of Agro Environmental Science and Technology, University of Bologna, I 40127 Bologna, Italy
    J Biol Chem 280:4684-95. 2005
  3. doi Chemistry of Ni2+ in urease: sensing, trafficking, and catalysis
    Barbara Zambelli
    Laboratory of Bioinorganic Chemistry, University of Bologna, Italy
    Acc Chem Res 44:520-30. 2011
  4. doi Helicobacter pylori UreE, a urease accessory protein: specific Ni(2+)- and Zn(2+)-binding properties and interaction with its cognate UreG
    Matteo Bellucci
    Laboratory of Bioinorganic Chemistry, Department of Agro Environmental Science and Technology, University of Bologna, Bologna, Italy
    Biochem J 422:91-100. 2009
  5. ncbi Structure of the intermolecular complex between plastocyanin and cytochrome f from spinach
    Francesco Musiani
    Laboratory of Bioinorganic Chemistry, Department of Agro Environmental Science and Technology, University of Bologna, Viale Giuseppe Fanin 40, 40127 Bologna, Italy
    J Biol Chem 280:18833-41. 2005
  6. doi Zn2+-linked dimerization of UreG from Helicobacter pylori, a chaperone involved in nickel trafficking and urease activation
    Barbara Zambelli
    Laboratory of Bioinorganic Chemistry, Department of Agro Environmental Science and Technology, University of Bologna, Bologna, Italy
    Proteins 74:222-39. 2009
  7. ncbi The nickel site of Bacillus pasteurii UreE, a urease metallo-chaperone, as revealed by metal-binding studies and X-ray absorption spectroscopy
    Massimiliano Stola
    Laboratory of Bioinorganic Chemistry, Department of Agro Environmental Science and Technology, University of Bologna, Viale Giuseppe Fanin 40, 40127 Bologna, Italy
    Biochemistry 45:6495-509. 2006
  8. doi Insights in the (un)structural organization of Bacillus pasteurii UreG, an intrinsically disordered GTPase enzyme
    Barbara Zambelli
    Laboratory of Bioinorganic Chemistry, University of Bologna, Bologna, Italy
    Mol Biosyst 8:220-8. 2012
  9. ncbi A model-based proposal for the role of UreF as a GTPase-activating protein in the urease active site biosynthesis
    Marco Salomone-Stagni
    Laboratory of Bioinorganic Chemistry, Department of Agro Environmental Science and Technology, University of Bologna, I 40127 Bologna, Italy
    Proteins 68:749-61. 2007
  10. ncbi Nickel trafficking: insights into the fold and function of UreE, a urease metallochaperone
    Francesco Musiani
    Department of Agro Environmental Science and Technology, University of Bologna, Viale Giuseppe Fanin 40, 40127 Bologna, Italy
    J Inorg Biochem 98:803-13. 2004

Collaborators

Detail Information

Publications31

  1. ncbi Molecular characterization of Bacillus pasteurii UreE, a metal-binding chaperone for the assembly of the urease active site
    Stefano Ciurli
    Department of Agro Environmental Science and Technology, University of Bologna, Via Filippo Re 8, Italy
    J Biol Inorg Chem 7:623-31. 2002
    ..Several secondary structure elements, including a betaalphabetabetaalphabeta "ferredoxin-like" motif, are highly conserved throughout the UreE sequences...
  2. ncbi UreG, a chaperone in the urease assembly process, is an intrinsically unstructured GTPase that specifically binds Zn2+
    Barbara Zambelli
    Laboratory of Bioinorganic Chemistry, Department of Agro Environmental Science and Technology, University of Bologna, I 40127 Bologna, Italy
    J Biol Chem 280:4684-95. 2005
    ..It is also proposed that metal ions such as Zn2+ could have important structural roles in the urease activation process...
  3. doi Chemistry of Ni2+ in urease: sensing, trafficking, and catalysis
    Barbara Zambelli
    Laboratory of Bioinorganic Chemistry, University of Bologna, Italy
    Acc Chem Res 44:520-30. 2011
    ....
  4. doi Helicobacter pylori UreE, a urease accessory protein: specific Ni(2+)- and Zn(2+)-binding properties and interaction with its cognate UreG
    Matteo Bellucci
    Laboratory of Bioinorganic Chemistry, Department of Agro Environmental Science and Technology, University of Bologna, Bologna, Italy
    Biochem J 422:91-100. 2009
    ..The results are discussed in relation to available evidence of a UreE-UreG functional interaction in vivo. A possible role for Zn(2+) in the Ni(2+)-dependent urease system is envisaged...
  5. ncbi Structure of the intermolecular complex between plastocyanin and cytochrome f from spinach
    Francesco Musiani
    Laboratory of Bioinorganic Chemistry, Department of Agro Environmental Science and Technology, University of Bologna, Viale Giuseppe Fanin 40, 40127 Bologna, Italy
    J Biol Chem 280:18833-41. 2005
    ....
  6. doi Zn2+-linked dimerization of UreG from Helicobacter pylori, a chaperone involved in nickel trafficking and urease activation
    Barbara Zambelli
    Laboratory of Bioinorganic Chemistry, Department of Agro Environmental Science and Technology, University of Bologna, Bologna, Italy
    Proteins 74:222-39. 2009
    ..The results are analyzed and discussed with respect to analogous examples of GTPases in nickel metabolism...
  7. ncbi The nickel site of Bacillus pasteurii UreE, a urease metallo-chaperone, as revealed by metal-binding studies and X-ray absorption spectroscopy
    Massimiliano Stola
    Laboratory of Bioinorganic Chemistry, Department of Agro Environmental Science and Technology, University of Bologna, Viale Giuseppe Fanin 40, 40127 Bologna, Italy
    Biochemistry 45:6495-509. 2006
    ..The arguments in favor of one model as compared to the other are discussed on the basis of the available biochemical data...
  8. doi Insights in the (un)structural organization of Bacillus pasteurii UreG, an intrinsically disordered GTPase enzyme
    Barbara Zambelli
    Laboratory of Bioinorganic Chemistry, University of Bologna, Bologna, Italy
    Mol Biosyst 8:220-8. 2012
    ..A possible role of disorder in the biological function of UreG is envisaged and discussed...
  9. ncbi A model-based proposal for the role of UreF as a GTPase-activating protein in the urease active site biosynthesis
    Marco Salomone-Stagni
    Laboratory of Bioinorganic Chemistry, Department of Agro Environmental Science and Technology, University of Bologna, I 40127 Bologna, Italy
    Proteins 68:749-61. 2007
    ..This result suggests a role for UreF in urease active site biosynthesis as a regulator of the activity of UreG, a small G protein involved in the in vivo apo-urease activation process and established to catalyze GTP hydrolysis...
  10. ncbi Nickel trafficking: insights into the fold and function of UreE, a urease metallochaperone
    Francesco Musiani
    Department of Agro Environmental Science and Technology, University of Bologna, Viale Giuseppe Fanin 40, 40127 Bologna, Italy
    J Inorg Biochem 98:803-13. 2004
    ....
  11. doi High-affinity Ni2+ binding selectively promotes binding of Helicobacter pylori NikR to its target urease promoter
    Barbara Zambelli
    Laboratory of Bioinorganic Chemistry, Department of Agro Environmental Science and Technology, University of Bologna, Bologna, Italy
    J Mol Biol 383:1129-43. 2008
    ..A general scheme for the nickel-selective HpNikR-DNA interaction is proposed...
  12. doi Model structures of Helicobacter pylori UreD(H) domains: a putative molecular recognition platform
    Francesco Musiani
    Laboratory of Bioinorganic Chemistry, University of Bologna, Viale G Fanin 40, 40127 Bologna, Italy
    J Chem Inf Model 51:1513-20. 2011
    ..The results suggest that UreD(H) acts as a multifunctional molecular recognition platform facilitating the interaction between apo-urease and the ancillary proteins UreG, UreF, and UreE, responsible for nickel trafficking and delivering...
  13. ncbi Intrinsically disordered structure of Bacillus pasteurii UreG as revealed by steady-state and time-resolved fluorescence spectroscopy
    Paolo Neyroz
    Department of Biochemistry G Moruzzi, University of Bologna, via San Donato 19 2, 40127 Bologna, Italy
    Biochemistry 45:8918-30. 2006
    ..The intrinsically disordered structure of UreG is discussed with respect to the role of this protein as a chaperone in the urease assembly system...
  14. ncbi Biochemical studies on Mycobacterium tuberculosis UreG and comparative modeling reveal structural and functional conservation among the bacterial UreG family
    Barbara Zambelli
    Laboratory of Bioinorganic Chemistry, Department of Agro Environmental Science and Technology, University of Bologna, Viale Giuseppe Fanin 40, 40127 Bologna, Italy
    Biochemistry 46:3171-82. 2007
    ..A flexible region, likely to be important for protein function, is identified. The structural conservation among this class of GTPases is discussed on the basis of their function in the urease assembly process...
  15. doi Intrinsic fluorescence of intrinsically disordered proteins
    Paolo Neyroz
    Department of Biochemistry G Moruzzi, University of Bologna, Rimini, Italy
    Methods Mol Biol 895:435-40. 2012
    ..Here, we describe the details of quenching fluorescence experiments and how to correlate the results to the peculiar structural information on the organization of intrinsically disordered proteins (IDPs)...
  16. doi Metal ion-mediated DNA-protein interactions
    Barbara Zambelli
    Department of Agro Environmental Science and Technology, University of Bologna, Bologna, Italy
    Met Ions Life Sci 10:135-70. 2012
    ..An overview of the recent advances in the understanding of how these proteins choose specific metal ions among the intracellular metal ion pool, as well as how they allosterically respond to their effector binding, is given...
  17. doi Structure of the UreD-UreF-UreG-UreE complex in Helicobacter pylori: a model study
    Francesco Biagi
    Laboratory of Bioinorganic Chemistry, Department of Pharmacy and Biotechnology, University of Bologna, Bologna, Italy
    J Biol Inorg Chem 18:571-7. 2013
    ....
  18. ncbi Electron transfer from HiPIP to the photooxidized tetraheme cytochrome subunit of Allochromatium vinosum reaction center: new insights from site-directed mutagenesis and computational studies
    Giovanni Venturoli
    Laboratorio di Biochimica e Biofisica, Dipartimento di Biologia, Universita di Bologna, Bologna, Italy, Istituto Nazionale per la Fisica della Materia INFM, UdR di Bologna, Bologna, Italy
    Biochemistry 43:437-45. 2004
    ..This conclusion was supported by protein docking calculations, resulting in a structural model for the HiPIP-THC complex, which involves a docking site close to the LP heme farthest from the bacteriochlorophyll special pair...
  19. ncbi Structural basis for the molecular properties of cytochrome c6
    Alexander Dikiy
    Department of Agro Environmental Science and Technology, University of Bologna, Via Filippo Re 8, Italy
    Biochemistry 41:14689-99. 2002
    ..In particular, the surface electrostatic potential of cytochromes c(6) features a hydrophobic region around the heme cofactor, and a backside surface rich in negative charges...
  20. doi Conformational fluctuations of UreG, an intrinsically disordered enzyme
    Francesco Musiani
    Laboratory of Bioinorganic Chemistry, Department of Pharmacy and Biotechnology, University of Bologna, Bologna, Italy
    Biochemistry 52:2949-54. 2013
    ..On the other hand, HpUreG tends to unfold more than MjHypB in portions involved in protein-protein interactions with metallochaperones necessary for the formation of multiprotein complexes known to be involved in urease activation...
  21. ncbi High potential iron-sulfur proteins and their role as soluble electron carriers in bacterial photosynthesis: tale of a discovery
    Stefano Ciurli
    Laboratory of Bioinorganic Chemistry, Department of Agro Environmental Science and Technology, University of Bologna Viale Giuseppe Fanin 40, 40127, Bologna, Italy
    Photosynth Res 85:115-31. 2005
    ....
  22. pmc The RNA hydrolysis and the cytokinin binding activities of PR-10 proteins are differently performed by two isoforms of the Pru p 1 peach major allergen and are possibly functionally related
    Paola Zubini
    Department of Agri Food Protection and Improvement, CRIOF, University of Bologna, 40127 Bologna, Italy
    Plant Physiol 150:1235-47. 2009
    ..The structural architecture of Pru p 1.01 and Pru p 1.06D was obtained by homology modeling, and the differences in the binding pockets, possibly accounting for the observed difference in binding activity, were evaluated...
  23. doi Interaction of selenoprotein W with 14-3-3 proteins: a computational approach
    Francesco Musiani
    Laboratory of Bioinorganic Chemistry, University of Bologna, Via Fanin 40, I 40127 Bologna, Italy
    J Proteome Res 10:968-76. 2011
    ....
  24. ncbi New insights into the mechanism of purple acid phosphatase through (1)H NMR spectroscopy of the recombinant human enzyme
    Alexander Dikiy
    Department of Agro Environmental Science and Technology, University of Bologna, Via Filippo Re 8, I 40127 Bologna, Italy
    J Am Chem Soc 124:13974-5. 2002
    ..The correlation between the pKa values observed in kinetics studies and for the spectroscopic changes strongly suggests that this group is the nucleophilic hydroxide that attacks the phosphate ester substrate...
  25. doi Selectivity of Ni(II) and Zn(II) binding to Sporosarcina pasteurii UreE, a metallochaperone in the urease assembly: a calorimetric and crystallographic study
    Barbara Zambelli
    Laboratory of Bioinorganic Chemistry, Department of Pharmacy and Biotechnology, University of Bologna, Bologna, Italy
    J Biol Inorg Chem 18:1005-17. 2013
    ..Overall, the thermodynamics and structural parameters that modulate the metal ion specificity of the different binding sites on the protein surface of SpUreE have been established. ..
  26. ncbi Structure of Rhodoferax fermentans high-potential iron-sulfur protein solved by MAD
    Ana Gonzalez
    Stanford Synchrotron Radiation Laboratory, 2575 Sand Hill Road MS99, Menlo Park, CA 94025, USA
    Acta Crystallogr D Biol Crystallogr 59:1582-8. 2003
    ....
  27. ncbi NMR solution structure, backbone mobility, and homology modeling of c-type cytochromes from gram-positive bacteria
    Lucia Banci
    Centro di Risonanze Magnetiche, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Italy
    Chembiochem 3:299-310. 2002
    ..At variance with mitochondrial c-type cytochrome, this protein does not experience pH-dependent coordination equilibria. The reasons for this difference are analyzed...
  28. doi Structural characterization of binding of Cu(II) to tau protein
    Alice Soragni
    Department for NMR based Structural Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Biochemistry 47:10841-51. 2008
    ..According to circular dichroism and NMR spectroscopy, Tau remains largely disordered upon binding to Cu(II), although a limited amount of aggregation is induced...
  29. ncbi Low-temperature EPR and Mössbauer spectroscopy of two cytochromes with His-Met axial coordination exhibiting HALS signals
    Giorgio Zoppellaro
    Department of Molecular Biosciences, University of Oslo, Box 1041 Blindern, 0316 Oslo, Norway
    Chemphyschem 7:1258-67. 2006
    ..Both EPR and Mössbauer data of axial cytochromes c with His-Met iron coordination are consistent with an electronic (d(xy))(2) (d(xz))(2) (d(yz))(1) ground state, which is typical for Type I model hemes...
  30. ncbi Jack bean (Canavalia ensiformis) urease. Probing acid-base groups of the active site by pH variation
    Barbara Krajewska
    Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30 060 Krakow, Poland
    Plant Physiol Biochem 43:651-8. 2005
    ....
  31. ncbi Molecular details of urease inhibition by boric acid: insights into the catalytic mechanism
    Stefano Benini
    York Structural Biology Laboratory, University of York, York, UK
    J Am Chem Soc 126:3714-5. 2004
    ..Boric acid can be considered a substrate analogue of urea, and the structure supports the proposal that the Ni-bridging hydroxide acts as the nucleophile in the enzymatic process of urea hydrolysis...