Research Topics
| Stefano CiurliSummaryAffiliation: University of Bologna Country: Italy Publications
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Detail Information
Publications
Molecular characterization of Bacillus pasteurii UreE, a metal-binding chaperone for the assembly of the urease active siteStefano Ciurli
Department of Agro Environmental Science and Technology, University of Bologna, Via Filippo Re 8, Italy
J Biol Inorg Chem 7:623-31. 2002..Several secondary structure elements, including a betaalphabetabetaalphabeta "ferredoxin-like" motif, are highly conserved throughout the UreE sequences...- UreG, a chaperone in the urease assembly process, is an intrinsically unstructured GTPase that specifically binds Zn2+Barbara Zambelli
Laboratory of Bioinorganic Chemistry, Department of Agro-Environmental Science and Technology, University of Bologna, I-40127 Bologna, Italy
J Biol Chem 280:4684-95. 2005..It is also proposed that metal ions such as Zn2+ could have important structural roles in the urease activation process... 
Chemistry of Ni2+ in urease: sensing, trafficking, and catalysisBarbara Zambelli
Laboratory of Bioinorganic Chemistry, University of Bologna, Italy
Acc Chem Res 44:520-30. 2011....- Helicobacter pylori UreE, a urease accessory protein: specific Ni(2+)- and Zn(2+)-binding properties and interaction with its cognate UreGMatteo Bellucci
Laboratory of Bioinorganic Chemistry, Department of Agro Environmental Science and Technology, University of Bologna, Bologna, Italy
Biochem J 422:91-100. 2009..The results are discussed in relation to available evidence of a UreE-UreG functional interaction in vivo. A possible role for Zn(2+) in the Ni(2+)-dependent urease system is envisaged... - Zn2+-linked dimerization of UreG from Helicobacter pylori, a chaperone involved in nickel trafficking and urease activationBarbara Zambelli
Laboratory of Bioinorganic Chemistry, Department of Agro Environmental Science and Technology, University of Bologna, Bologna, Italy
Proteins 74:222-39. 2009..The results are analyzed and discussed with respect to analogous examples of GTPases in nickel metabolism... - Structure of the intermolecular complex between plastocyanin and cytochrome f from spinachFrancesco Musiani
Laboratory of Bioinorganic Chemistry, Department of Agro-Environmental Science and Technology, University of Bologna, Viale Giuseppe Fanin 40, 40127 Bologna, Italy
J Biol Chem 280:18833-41. 2005.... - The nickel site of Bacillus pasteurii UreE, a urease metallo-chaperone, as revealed by metal-binding studies and X-ray absorption spectroscopyMassimiliano Stola
Laboratory of Bioinorganic Chemistry, Department of Agro-Environmental Science and Technology, University of Bologna, Viale Giuseppe Fanin 40, 40127 Bologna, Italy
Biochemistry 45:6495-509. 2006..The arguments in favor of one model as compared to the other are discussed on the basis of the available biochemical data... - Insights in the (un)structural organization of Bacillus pasteurii UreG, an intrinsically disordered GTPase enzymeBarbara Zambelli
Laboratory of Bioinorganic Chemistry, University of Bologna, Bologna, Italy
Mol Biosyst 8:220-8. 2012..A possible role of disorder in the biological function of UreG is envisaged and discussed... - A model-based proposal for the role of UreF as a GTPase-activating protein in the urease active site biosynthesisMarco Salomone Stagni
Laboratory of Bioinorganic Chemistry, Department of Agro Environmental Science and Technology, University of Bologna, I 40127 Bologna, Italy
Proteins 68:749-61. 2007..This result suggests a role for UreF in urease active site biosynthesis as a regulator of the activity of UreG, a small G protein involved in the in vivo apo-urease activation process and established to catalyze GTP hydrolysis... - Nickel trafficking: insights into the fold and function of UreE, a urease metallochaperoneFrancesco Musiani
Department of Agro-Environmental Science and Technology, University of Bologna, Viale Giuseppe Fanin 40, 40127 Bologna, Italy
J Inorg Biochem 98:803-13. 2004.... - High-affinity Ni2+ binding selectively promotes binding of Helicobacter pylori NikR to its target urease promoterBarbara Zambelli
Laboratory of Bioinorganic Chemistry, Department of Agro Environmental Science and Technology, University of Bologna, Bologna, Italy
J Mol Biol 383:1129-43. 2008..A general scheme for the nickel-selective HpNikR-DNA interaction is proposed... - Model structures of Helicobacter pylori UreD(H) domains: a putative molecular recognition platformFrancesco Musiani
Laboratory of Bioinorganic Chemistry, University of Bologna, Viale G Fanin 40, 40127 Bologna, Italy
J Chem Inf Model 51:1513-20. 2011..The results suggest that UreD(H) acts as a multifunctional molecular recognition platform facilitating the interaction between apo-urease and the ancillary proteins UreG, UreF, and UreE, responsible for nickel trafficking and delivering... - Biochemical studies on Mycobacterium tuberculosis UreG and comparative modeling reveal structural and functional conservation among the bacterial UreG familyBarbara Zambelli
Laboratory of Bioinorganic Chemistry, Department of Agro Environmental Science and Technology, University of Bologna, Viale Giuseppe Fanin 40, 40127 Bologna, Italy
Biochemistry 46:3171-82. 2007..A flexible region, likely to be important for protein function, is identified. The structural conservation among this class of GTPases is discussed on the basis of their function in the urease assembly process... - Intrinsically disordered structure of Bacillus pasteurii UreG as revealed by steady-state and time-resolved fluorescence spectroscopyPaolo Neyroz
Department of Biochemistry G. Moruzzi, University of Bologna, Via San Donato 19/2, 40127 Bologna, Italy
Biochemistry 45:8918-30. 2006..The intrinsically disordered structure of UreG is discussed with respect to the role of this protein as a chaperone in the urease assembly system... - Intrinsic fluorescence of intrinsically disordered proteinsPaolo Neyroz
Department of Biochemistry G Moruzzi, University of Bologna, Rimini, Italy
Methods Mol Biol 895:435-40. 2012..Here, we describe the details of quenching fluorescence experiments and how to correlate the results to the peculiar structural information on the organization of intrinsically disordered proteins (IDPs)... - Metal ion-mediated DNA-protein interactionsBarbara Zambelli
Department of Agro Environmental Science and Technology, University of Bologna, Bologna, Italy
Met Ions Life Sci 10:135-70. 2012..An overview of the recent advances in the understanding of how these proteins choose specific metal ions among the intracellular metal ion pool, as well as how they allosterically respond to their effector binding, is given... - Electron transfer from HiPIP to the photooxidized tetraheme cytochrome subunit of Allochromatium vinosum reaction center: new insights from site-directed mutagenesis and computational studiesGiovanni Venturoli
Laboratorio di Biochimica e Biofisica, Dipartimento di Biologia, , Bologna, Italy, Istituto Nazionale per la Fisica della Materia (INFM, UdR di Bologna, Bologna, Italy
Biochemistry 43:437-45. 2004..This conclusion was supported by protein docking calculations, resulting in a structural model for the HiPIP-THC complex, which involves a docking site close to the LP heme farthest from the bacteriochlorophyll special pair... - Structural basis for the molecular properties of cytochrome c6Alexander Dikiy
Department of Agro-Environmental Science and Technology, University of Bologna, Via Filippo Re 8, Italy
Biochemistry 41:14689-99. 2002..In particular, the surface electrostatic potential of cytochromes c(6) features a hydrophobic region around the heme cofactor, and a backside surface rich in negative charges... - High potential iron-sulfur proteins and their role as soluble electron carriers in bacterial photosynthesis: tale of a discoveryStefano Ciurli
Laboratory of Bioinorganic Chemistry, Department of Agro Environmental Science and Technology, University of Bologna Viale Giuseppe Fanin 40, 40127, Bologna, Italy
Photosynth Res 85:115-31. 2005.... 
The RNA hydrolysis and the cytokinin binding activities of PR-10 proteins are differently performed by two isoforms of the Pru p 1 peach major allergen and are possibly functionally relatedPaola Zubini
Department of Agri Food Protection and Improvement, CRIOF, University of Bologna, 40127 Bologna, Italy
Plant Physiol 150:1235-47. 2009..The structural architecture of Pru p 1.01 and Pru p 1.06D was obtained by homology modeling, and the differences in the binding pockets, possibly accounting for the observed difference in binding activity, were evaluated...- Interaction of selenoprotein W with 14-3-3 proteins: a computational approachFrancesco Musiani
Laboratory of Bioinorganic Chemistry, University of Bologna, Via Fanin 40, I 40127 Bologna, Italy
J Proteome Res 10:968-76. 2011.... - New insights into the mechanism of purple acid phosphatase through (1)H NMR spectroscopy of the recombinant human enzymeAlexander Dikiy
Department of Agro-Environmental Science and Technology, University of Bologna, Via Filippo Re 8, I-40127 Bologna, Italy
J Am Chem Soc 124:13974-5. 2002..The correlation between the pKa values observed in kinetics studies and for the spectroscopic changes strongly suggests that this group is the nucleophilic hydroxide that attacks the phosphate ester substrate... - Structure of Rhodoferax fermentans high-potential iron-sulfur protein solved by MADAna Gonzalez
Stanford Synchrotron Radiation Laboratory, 2575 Sand Hill Road MS99, Menlo Park, CA 94025, USA
Acta Crystallogr D Biol Crystallogr 59:1582-8. 2003.... - NMR solution structure, backbone mobility, and homology modeling of c-type cytochromes from gram-positive bacteriaLucia Banci
Centro di Risonanze Magnetiche, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Italy
Chembiochem 3:299-310. 2002..At variance with mitochondrial c-type cytochrome, this protein does not experience pH-dependent coordination equilibria. The reasons for this difference are analyzed... - Structural characterization of binding of Cu(II) to tau proteinAlice Soragni
Department for NMR based Structural Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
Biochemistry 47:10841-51. 2008..According to circular dichroism and NMR spectroscopy, Tau remains largely disordered upon binding to Cu(II), although a limited amount of aggregation is induced... - Molecular details of urease inhibition by boric acid: insights into the catalytic mechanismStefano Benini
York Structural Biology Laboratory, University of York, York, UK
J Am Chem Soc 126:3714-5. 2004..Boric acid can be considered a substrate analogue of urea, and the structure supports the proposal that the Ni-bridging hydroxide acts as the nucleophile in the enzymatic process of urea hydrolysis... - Jack bean (Canavalia ensiformis) urease. Probing acid-base groups of the active site by pH variationBarbara Krajewska
Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30 060 Krakow, Poland
Plant Physiol Biochem 43:651-8. 2005.... - Low-temperature EPR and Mössbauer spectroscopy of two cytochromes with His-Met axial coordination exhibiting HALS signalsGiorgio Zoppellaro
Department of Molecular Biosciences, University of Oslo, Box 1041 Blindern, 0316 Oslo, Norway
Chemphyschem 7:1258-67. 2006..Both EPR and Mössbauer data of axial cytochromes c with His-Met iron coordination are consistent with an electronic (d(xy))(2) (d(xz))(2) (d(yz))(1) ground state, which is typical for Type I model hemes...