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| Pier Carlo BragaSummaryAffiliation: University of Milan Country: Italy Publications
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Publications
Free radical-scavenging activity of sulfurous water investigated by electron paramagnetic resonance (EPR) spectroscopyPier Carlo Braga
Center of Respiratory Pharmacology, Department of Pharmacology, School of Medicine, University of Milan, Milan, Italy
Exp Lung Res 38:67-74. 2012....
Characterisation of the antioxidant effects of Aesculus hippocastanum L. bark extract on the basis of radical scavenging activity, the chemiluminescence of human neutrophil bursts and lipoperoxidation assayP C Braga
Department of Pharmacology, School of Medicine, University of Milan, Milan, Italy
Eur Rev Med Pharmacol Sci 16:1-9. 2012..It has been claimed that Aesculus hippocastanum extracts have antioxidant and anti-inflammatory activity, but these claims are mainly based on the results of chemical reactions and folk-medicine...
Inhibitory activity of thymol against the formation and viability of Candida albicans hyphaeP C Braga
Department of Pharmacology, School of Medicine, University of Milan, Milan, Italy
Mycoses 50:502-6. 2007..This can be attributed to the characteristics of thymol disturbing Candida cell membranes and metabolism, probably by affecting fungal cell-wall synthesising enzymes...
Antioxidant effect of sulphurous thermal water on human neutrophil bursts: chemiluminescence evaluationPier Carlo Braga
Center of Respiratory Pharmacology, Department of Pharmacology, School of Medicine, University of Milan, Milan, Italy
Respiration 75:193-201. 2008....
Eugenol and thymol, alone or in combination, induce morphological alterations in the envelope of Candida albicansP C Braga
Department of Pharmacology, School of Medicine, University of Milano, Milano, Italy
Fitoterapia 78:396-400. 2007..Certain combinations of the two molecules led to a synergistic effect, which is interesting in the view of potentiating their inhibition of C. albicans colonisation and infectiousness...
Differences in the susceptibility of Streptococcus pyogenes to rokitamycin and erythromycin A revealed by morphostructural atomic force microscopyPier Carlo Braga
Centre of Respiratory Pharmacology, Department of Pharmacology, School of Medicine, University of Milan, Via Vanvitelli 32, 20129 Milano, Italy
J Antimicrob Chemother 50:457-60. 2002..pyogenes, whereas rokitamycin has a progressive action that leads to the formation of abnormally large cells, the loosening of chain structure and the formation of clusters...
Effect of metabolite I of erdosteine on the release of human neutrophil elastasePier Carlo Braga
Center of Respiratory Pharmacology, Department of Pharmacology, School of Medicine, University of Milan, Milan, Italy
Pharmacology 77:150-4. 2006..By reducing elastase and oxidant radical release (two major components of the inflammatory process), Met I have clinically useful anti-inflammatory effects...
Budesonide reduces superoxide and peroxynitrite anion chemiluminescence during human neutrophil burstsPier Carlo Braga
Center of Respiratory Pharmacology, Department of Pharmacology, School of Medicine, University of Milan, Milan, Italy
Pharmacology 75:179-86. 2005....
Anti-inflammatory activity of thymol: inhibitory effect on the release of human neutrophil elastasePier Carlo Braga
Department of Pharmacology, School of Medicine, University of Milan, Milan, Italy
Pharmacology 77:130-6. 2006..These two latter characteristics make thymol a molecule that can have helpful effects in controlling the inflammatory processes present in many infections...
Antioxidant potential of thymol determined by chemiluminescence inhibition in human neutrophils and cell-free systemsPier Carlo Braga
Center of Respiratory Pharmacology, Department of Pharmacology, School of Medicine, University of Milan, Via Vanvitelli 32, IT 20129 Milan, Italy
Pharmacology 76:61-8. 2006..Our findings in human neutrophils are pharmacologically relevant as they imply that thymol is a potential antioxidant and anti-inflammatory agent in human cells...
Effect of butylated hydroxyanisole and some of its derivatives on human neutrophil oxidative burst: chemiluminescence evaluationPier Carlo Braga
Department of Pharmacology, School of Medicine, University of Milan, Italy
Pharmacology 68:9-16. 2003..e. free radical release and Ca(2+) overload...
The post-antibiotic effects of rokitamycin (a 16-membered ring macrolide) on susceptible and erythromycin-resistant strains of Streptococcus pyogenesP C Braga
Center of Respiratory Pharmacology, Department of Pharmacology, School of Medicine, University of Milan, Via Vanvitelli 32, 20129 Milano, Italy
Int J Antimicrob Agents 24:254-60. 2004..They also indicated that, as the PAEs of rokitamycin on the M phenotype and inducible resistant strains were comparable with those on susceptible strains, no re-evaluation of therapeutic dosing regimens was required...
Thymol inhibits Candida albicans biofilm formation and mature biofilmPier Carlo Braga
Department of Pharmacology, School of Medicine, University of Milan, Via Vanvitelli 32, Milan, Italy
Int J Antimicrob Agents 31:472-7. 2008..As biofilm is a multifactorial phenomenon, the multiple mechanisms of thymol (terpenes) could act on different steps in the evolution of mature biofilm...
Visual evaluation of binding to mucosal cells of a medical device against the common coldPier Carlo Braga
Department of Pharmacology, School of Medicine, Center of Respiratory Pharmacology, University of Milan, Milan, Italy
Drug Dev Ind Pharm 34:459-64. 2008....
Antioxidant activity of bisabolol: inhibitory effects on chemiluminescence of human neutrophil bursts and cell-free systemsPier Carlo Braga
Department of Pharmacology, School of Medicine, University of Milan, Milan, Italy
Pharmacology 83:110-5. 2009..These findings draw the attention to the possible medical use of bisabolol as a means of improving the antioxidant network and restoring the redox balance by antagonising oxidative stress...
Thymol-induced alterations in Candida albicans imaged by atomic force microscopyPier Carlo Braga
Department of Pharmacology, School of Medicine, University of Milan, Milan, Italy
Methods Mol Biol 736:401-10. 2011..All of these phenomena lead to major surface alterations and deformities that also reduce the ability of fungi to adhere to mucosal cells, and decrease their virulence and infectiousness...
Imaging bacterial shape, surface, and appendages before and after treatment with antibioticsPier Carlo Braga
Department of Pharmacology, School of Medicine, University of Milan, Via Vanvitelli 32, Milan 20129, Italy
Methods Mol Biol 736:391-9. 2011..e., no vacuum is necessary), and effective three-dimensional magnification at submicron level...
Inhibitory activity of thymol on native and mature Gardnerella vaginalis biofilms: in vitro studyPier Carlo Braga
Department of Pharmacology, School of Medicine, University of Milan, Italy
Arzneimittelforschung 60:675-81. 2010..Nomarski interference contrast and fluorescence microscopy visually confirmed these findings. As biofilm is a multi-factorial phenomenon, the multiple mechanisms of thymol may act on different steps in the evolution of mature biofilm...
Morphostructural damage and the inhibition of bacterial adhesiveness of Staphylococcus aureus and Moraxella catarrhalis induced by moxifloxacinP C Braga
Department of Pharmacology, School of Medicine, University of Milan, Italy
J Chemother 15:543-50. 2003..Our findings suggest that less than conventional MIC moxifloxacin concentrations may be effective in reducing bacterial adhesiveness and structural integrity on which the maintenance of bacterial activity depends...
Effects of sulphurous water on human neutrophil elastase releasePier Carlo Braga
Center of Respiratory Pharmacology, Department of Pharmacology, School of Medicine, University of Milan, Via Vanvitelli 32, 20129 Milan, Italy
Ther Adv Respir Dis 4:333-40. 2010..The aim of this study was to investigate the direct activity of such water on the release of elastase by activated human neutrophils...
Free radical scavenging activity of erdosteine metabolite I investigated by electron paramagnetic resonance spectroscopyPier Carlo Braga
Department of Pharmacology, School of Medicine, University of Milan, Milan, Italy
Pharmacology 85:195-202. 2010..Our findings show that the intensity of 4 different free radicals was significantly reduced in the presence of Met I, thus indicating the presence of a termination reaction between the free radicals and Met I...
Antioxidant activity of Calendula officinalis extract: inhibitory effects on chemiluminescence of human neutrophil bursts and electron paramagnetic resonance spectroscopyPier Carlo Braga
Department of Pharmacology, School of Medicine, University of Milan, Milan, Italy
Pharmacology 83:348-55. 2009....
Vaginal gel adsorption and retention by human vaginal cells: visual analysis by means of inorganic and organic markersPier Carlo Braga
Department of Pharmacology, School of Medicine, University of Milan, Via Vanvitelli 32, 20129 Milan, Italy
Int J Pharm 373:10-5. 2009....
Visual analysis of vaginal cell binding and retention of a gynecological doucheP C Braga
Department of Pharmacology, School of Medicine, University of Milan, Milan, Italy
Minerva Ginecol 62:7-13, 13-6. 2010..The aim of this study was to investigate the possibility of directly visualising the ability of a commercial medical gynecological douche to bind to, and be retained by human vaginal cells...
Human neutrophil oxidative bursts and their in vitro modulation by different N-acetylcysteine concentrationsLuigi Allegra
Department of Pharmacology, Institute of Respiratory Diseases, School of Medicine, University of Milan, Milan, Italy
Arzneimittelforschung 52:669-76. 2002..The fact that no effects were seen on PMN phagocytosis and bacterial killing indicates that NAC has no negative influence on other PMN functions such as antimicrobial activity...
Inhibitory effects of metabolite I of erdosteine on the generation of nitric oxide and peroxynitrite chemiluminescence by human neutrophilsMonica Dal Sasso
Center of Respiratory Pharmacology, Department of Pharmacology, School of Medicine, University of Milan, Milan, Italy
Pharmacology 71:120-7. 2004....
A combination of budesonide and the SH-metabolite I of erdosteine acts synergistically in reducing chemiluminescence during human neutrophil respiratory burstMonica Dal Sasso
Center of Respiratory Pharmacology, Department of Pharmacology, School of Medicine, University of Milan, Milan, Italy
Pharmacology 74:127-34. 2005..Our findings indicate a synergistic antioxidant effect when BUD and Met I are given together, which is of interest for counteracting the airway phlogosis involved in many respiratory diseases...
Protective effect of erdosteine metabolite I against hydrogen peroxide-induced oxidative DNA-damage in lung epithelial cellsLaura Marabini
Department of Pharmacology, Chemotherapy and Medical Toxicology, School of Medicine, University of Milan, Milan, Italy
Arzneimittelforschung 61:700-6. 2011..The data suggest that this compound is effective in preventing H2O2-induced oxidative stress and DNA damage in A549 cells. The underlying mechanisms involve the scavenging of intracellular reactive oxygen species (ROS)...
Gemifloxacin: effects of sub-inhibitory concentrations on various factors affecting bacterial virulenceM Dal Sasso
Department of Pharmacology, Center of Respiratory Pharmacology, School of Medicine, University of Milan, Via Vanvitelli 32, 20129 Milan, Italy
Int J Antimicrob Agents 21:325-33. 2003....
Thermal treatment of eggplant (Solanum melongena L.) increases the antioxidant content and the inhibitory effect on human neutrophil burstRoberto Lo Scalzo
CRA IAA, Agricultural Research Council, Food Technology Research Unit, via Venezian 26, Milan, Italy
J Agric Food Chem 58:3371-9. 2010..00 microg/mL. These results showed that the thermal treatment commonly used before consumption can increase the content and biological activity of antioxidant compounds of eggplants...
The combination of the SH metabolite of erdosteine (a mucoactive drug) and ciprofloxacin increases the inhibition of bacterial adhesiveness achieved by ciprofloxacin aloneSasso M Dal
Center for Respiratory Pharmacology, Department of Pharmacology, School of Medicine, University of Milan, Italy
Drugs Exp Clin Res 28:75-82. 2002..This finding opens up an interesting new possibility for interfering with bacterial adhesiveness and the resulting virulence by combining antibiotics with agents devoid of antibacterial activity...
Flow cytometric assessment of susceptibilities of Streptococcus pyogenes to erythromycin and rokitamycinPier Carlo Braga
Center of Respiratory Pharmacology, Department of Pharmacology, School of Medicine, University of Milan, Milan, Italy
Antimicrob Agents Chemother 47:408-12. 2003..pyogenes M phenotype, and of erythromycin at doses of up to 32 microg/ml, which was not...
Interference of sub-inhibitory concentrations of gatifloxacin on various determinants of bacterial virulenceM Dal Sasso
Center of Respiratory Pharmacology, Department of Pharmacology, School of Medicine, University of Milan, Milan, Italy
J Chemother 14:473-82. 2002....
Imaging bacterial shape, surface, and appendages before and after treatments with antibioticsPier Carlo Braga
Department of Pharmacology and Center of Respiratory Pharmacology, School of Medicine, University of Milan, Italy
Methods Mol Biol 242:179-88. 2004
Effects of sub-minimum inhibitory concentrations of gatifloxacin on the inhibition of Staphylococcus aureus and Escherichia coli adherencePier Carlo Braga
Center of Respiratory Pharmacology, Department of Pharmacology, School of Medicine, University of Milan, Milan, Italy
Arzneimittelforschung 52:109-12. 2002..aureus and E. coli for as long as 30 h and 37 h, respectively, after the MIC values have been reached...
Inhibition of bacterial adhesion by sub-inhibitory concentrations: brodimoprim vs trimethoprimP C Braga
Dept of Pharmacology, School of Medicine, University of Milan, Italy
J Chemother 5:447-52. 1993....
Phagocytosis and intracellular killing of rokitamycin-exposed staphylococci by human polymorphonuclear leukocytesP C Braga
Department of Pharmacology, School of Medicine, University of Milan, Italy
Chemotherapy 44:42-9. 1998..This effect can be correlated with the high uptake of RKM by PMNs (cellular/extracellular ratio congruent to 30.5). The relevance of these in vitro observations to clinical situations remains to be further investigated...
Bacterial adhesiveness: effects of the SH metabolite of erdosteine (mucoactive drug) plus clarithromycin versus clarithromycin aloneP C Braga
Department of Pharmacology, School of Medicine, University of Milan, Milan, Italy
Chemotherapy 47:208-14. 2001..This finding opens up a new possibility of interfering with bacterial adhesiveness and its resulting pathogenicity not only by using antibiotics but also by means of their combination with agents devoid of antibacterial activity...
Inhibitory effects of zafirlukast on respiratory bursts of human neutrophilsP C Braga
Center of Respiratory Pharmacology, Department of Pharmacology, School of Medicine, University of Milan, Milan, Italy
Drugs Exp Clin Res 28:133-45. 2002..The different behavior determined by different times of contact and different stimuli opens the way to interpretations concerning the antioxidant effect of zafirlukast...
Effects of rokitamycin on phagocytosis and release of oxidant radicals of human polymorphonuclear leukocytesP C Braga
Department of Pharmacology, School of Medicine, University of Milan, Italy
Chemotherapy 43:190-7. 1997..RKM has no unwanted effects on PMNs because the serum concentrations that can be obtained with the highest doses administered to man are lower than the concentrations which did not affect PMN functions in our study...
Atomic force microscopy: application to investigation of Escherichia coli morphology before and after exposure to cefodizimeP C Braga
Center for Respiratory Pharmacology, School of Medicine, University of Milan, Italy
Antimicrob Agents Chemother 42:18-22. 1998....
Pharmacodynamic effects of subinhibitory concentrations of rufloxacin on bacterial virulence factorsP C Braga
Department of Pharmacology, School of Medicine, University of Milan, Milan, Italy
Antimicrob Agents Chemother 43:1013-9. 1999....
Assessment of the antioxidant activity of the SH metabolite I of erdosteine on human neutrophil oxidative burstsP C Braga
Center for Respiratory Pharmacology, School of Medicine, University of Milan, Italy
Arzneimittelforschung 50:739-46. 2000..The findings indicate that the metabolite I of erdosteine has antioxidant activity which, together with the drug's mucomodifying activity, may lead to a useful antiphlogistic effect...
The SH-metabolite I of erdosteine, a mucolytic drug, enhances the inhibitory effect of salbutamol on the respiratory burst of neutrophilsM Dal Sasso
Center of Respiratory Pharmacology, Department of Pharmacology, School of Medicine, University of Milan, Milan, Italy
Drugs Exp Clin Res 28:147-54. 2002..The reasons for this different behavior remain unclear and raise questions about the specific roles, sites and mechanisms of action of the different types of stimulation undergone by the respiratory airways...
Staphylococcus aureus and Escherichia coli adhesion to human cells is reduced by sub-MICs of gemifloxacinP C Braga
Center of Respiratory Pharmacology, Department of Pharmacology, School of Medicine, University of Milan, Italy
J Chemother 14:41-6. 2002..aureus were also observed, mainly from 1/2 MIC to 1/8 and 1/16 MIC. These findings are discussed in terms of dose-effect relationships and the interpolation of this pharmacodynamic data with the pharmacokinetics curve of gemifloxacin...
Effects of gatifloxacin on phagocytosis, intracellular killing and oxidant radical production by human polymorphonuclear neutrophilsP C Braga
Center for Respiratory Pharmacology, Department of Pharmacology, School of Medicine, University of Milan, Via Vanvitelli 32, 20129 Milan, Italy
Int J Antimicrob Agents 19:183-7. 2002..coli strain at 1/32 MIC and in two strains at 1/16 MIC. The incubation of PMNs with sub-MICs and supra-MICs of gatifloxacin (to 32 MIC) did not affect the oxidative bursts...
Relationship between differences in 16-, 14-, and 15-membered ring macrolides and Streptococcus pyogenes resistanceP C Braga
Centre of Respiratory Pharmacology, Department of Pharmacology, University of Milan, Italy
Eur J Clin Microbiol Infect Dis 21:146-8. 2002
Rokitamycin: bacterial resistance to a 16-membered ring macrolide differs from that to 14- and 15-membered ring macrolidesP C Braga
Department of Pharmacology, School of Medicine, University of Milan, Italy
J Chemother 14:115-31. 2002....
Daptomycin morphostructural damage in Bacillus cereus visualized by atomic force microscopyP C Braga
Department of Pharmacology, School of Medicine, University of Milan, Italy
J Chemother 14:336-41. 2002..In addition to structural changes, the destabilization of flagella was also observed. These results support previous data suggesting that daptomycin disrupts membrane function...
Sub-lethal concentrations of clarithromycin interfere with the expression of Staphylococcus aureus adhesiveness to human cellsP C Braga
Center for Respiratory Pharmacology, School of Medicine, University of Milan, Italy
J Chemother 5:159-63. 1993..These are ligand molecules located on the surface of bacteria, and thus reduce the ability of bacteria to bind specifically to complementary molecules on the surfaces of epithelial cells which is necessary for host colonization...
Age-associated differences in neutrophil oxidative burst (chemiluminescence)P C Braga
Department of Pharmacology, School of Medicine, University of Milan, Italy
Exp Gerontol 33:477-84. 1998....
Alterations in surface morphology of Candida albicans produced by rilopirox: a scanning electron microscopy studyP C Braga
Department of Pharmacology, University of Milan, Italy
J Chemother 7:519-24. 1995..The effects of rilopirox are already evident at 1 x MIC and after 1 h but their frequency and severity are correlated with the time of incubation and the MIC...
Restoration of polymorphonuclear leukocyte function in elderly subjects by thymomodulinP C Braga
Dept of Pharmacology, School of Medicine, University of Milan, Italy
J Chemother 6:354-9. 1994..Luminol-dependent chemiluminescence was increased by 15.6%, which was not significant, indicating a different response between phagocytosis and release of oxidant radicals...
Detection of rokitamycin-induced morphostructural alterations in Helicobacter pylori by atomic force microscopyP C Braga
Centre for Respiratory Pharmacology, School of Medicine, University of Milan, Italy
Chemotherapy 46:15-22. 2000....
Thymomodulin stimulates phagocytosis in vitro by rat macrophages and human polymorphonuclear cellsP C Braga
Department of Pharmacology, School of Medicine, University of Milan, Italy
J Chemother 5:313-6. 1993....
Imaging methods in atomic force microscopyDavide Ricci
Department of Biophysical and Electronic Engineering, University of Genoa, Italy
Methods Mol Biol 242:13-23. 2004
Recognizing and avoiding artifacts in AFM imagingDavide Ricci
Department of Biophysical and Electronic Engineering, University of Genoa, Italy
Methods Mol Biol 242:25-37. 2004
How the atomic force microscope worksDavide Ricci
Department of Biophysical and Electronic Engineering, University of Genoa, Italy
Methods Mol Biol 242:3-12. 2004
