N Borgese

Summary

Affiliation: University of Milan
Country: Italy

Publications

  1. ncbi request reprint Trafficking of tail-anchored proteins: transport from the endoplasmic reticulum to the plasma membrane and sorting between surface domains in polarised epithelial cells
    Alessandra Bulbarelli
    Consiglio Nazionale delle Ricerche Cellular and Molecular Pharmacology Center and Department of Medical Pharmacology, University of Milan, Milan, Italy
    J Cell Sci 115:1689-702. 2002
  2. pmc KDEL and KKXX retrieval signals appended to the same reporter protein determine different trafficking between endoplasmic reticulum, intermediate compartment, and Golgi complex
    Mariano Stornaiuolo
    Department of Biochemistry and Medical Biotechnology, University of Naples Federico II, Italy
    Mol Biol Cell 14:889-902. 2003
  3. pmc The tale of tail-anchored proteins: coming from the cytosol and looking for a membrane
    Nica Borgese
    Consiglio Nazionale delle Ricerche Institute for Neuroscience, Cellular and Molecular Pharmacology Section, Via Vanvitelli 32, 20129 Milano, Italy
    J Cell Biol 161:1013-9. 2003
  4. pmc Transmembrane topogenesis of a tail-anchored protein is modulated by membrane lipid composition
    Silvia Brambillasca
    CNR Institute of Neuroscience Cell Mol Pharmacology and Department of Medical Pharmacology, University of Milan, Milan, Italy
    EMBO J 24:2533-42. 2005
  5. ncbi request reprint How tails guide tail-anchored proteins to their destinations
    Nica Borgese
    National Research Council Institute for Neuroscience and Department of Medical Pharmacology, University of Milan, Via Vanvitelli 32, 20129 Milano, Italy
    Curr Opin Cell Biol 19:368-75. 2007
  6. doi request reprint Targeting pathways of C-tail-anchored proteins
    Nica Borgese
    National Research Council Institute for Neuroscience and Department of Medical Pharmacology, University of Milan, Milano, Italy
    Biochim Biophys Acta 1808:937-46. 2011
  7. ncbi request reprint Biogenesis of tail-anchored proteins
    N Borgese
    Consiglio Nazionale delle Ricerche Institute for Neuroscience, Cellular and Molecular Pharmacology Section, University of Milan, Via Vanvitelli 32, 20129 Milan, Italy
    Biochem Soc Trans 31:1238-42. 2003
  8. doi request reprint Remote origins of tail-anchored proteins
    Nica Borgese
    Consiglio Nazionale delle Ricerche Institute of Neuroscience and Department of Pharmacology, Universita degli Studi di Milano, Via Vanvitelli 32, 20129 Milano, Italy
    Traffic 11:877-85. 2010
  9. ncbi request reprint Endoplasmic reticulum architecture: structures in flux
    Nica Borgese
    National Research Council Institute for Neuroscience and Department of Medical Pharmacology, University of Milan, Via Vanvitelli 32, 20129 Milano, Italy
    Curr Opin Cell Biol 18:358-64. 2006
  10. pmc Targeting of a tail-anchored protein to endoplasmic reticulum and mitochondrial outer membrane by independent but competing pathways
    N Borgese
    Consiglio Nazionale delle Ricerche, Cellular and Molecular Pharmacology Center and Department of Medical Pharmacology, University of Milan, Milan, Italy
    Mol Biol Cell 12:2482-96. 2001

Collaborators

  • Maura Francolini
  • S Alcaro
  • Ramanujan S Hegde
  • Jennifer Lippincott-Schwartz
  • Silvia Brambillasca
  • Sara Colombo
  • Teresa Sprocati
  • Monica Yabal
  • Paolo Ronchi
  • Marja Makarow
  • Elisa Fasana
  • Sara F Colombo
  • Emanuela Pedrazzini
  • Renato Longhi
  • Stefania Bulotta
  • Paolo Ceppi
  • Paolo Soffientini
  • Mariano Stornaiuolo
  • Erik L Snapp
  • Alessandra Bulbarelli
  • Annamaria Ruggiano
  • Francesca Navone
  • Matteo Fossati
  • Casper C Hoogenraad
  • Rico Barsacchi
  • Andrea Raimondi
  • Emilio Clementi
  • Domenicantonio Rotiroti
  • Andrea Cerullo
  • A Bulbarelli
  • Clara De Palma
  • Massimo Masserini
  • Adriano Flora
  • Francesca Raimondo
  • Francesco Ortuso
  • Sandra Stefanovic
  • Maria Rosaria Torrisi
  • Gianluca Martire
  • Lavinia V Lotti
  • Francesca Lombardo
  • Stefano Bonatti
  • Giovanna Mottola
  • Massimo Barberi
  • M De Silvestris
  • M D Cappellini
  • A Valentini
  • M DeSilvestris
  • A D'Arrigo

Detail Information

Publications23

  1. ncbi request reprint Trafficking of tail-anchored proteins: transport from the endoplasmic reticulum to the plasma membrane and sorting between surface domains in polarised epithelial cells
    Alessandra Bulbarelli
    Consiglio Nazionale delle Ricerche Cellular and Molecular Pharmacology Center and Department of Medical Pharmacology, University of Milan, Milan, Italy
    J Cell Sci 115:1689-702. 2002
    ..The two GFP fusion proteins showed a different surface distribution, which is consistent with a role for the two syntaxin TMDs in polarised sorting...
  2. pmc KDEL and KKXX retrieval signals appended to the same reporter protein determine different trafficking between endoplasmic reticulum, intermediate compartment, and Golgi complex
    Mariano Stornaiuolo
    Department of Biochemistry and Medical Biotechnology, University of Naples Federico II, Italy
    Mol Biol Cell 14:889-902. 2003
    ..These results illustrate how different retrieval signals determine different trafficking patterns and pose novel questions on the underlying molecular mechanisms...
  3. pmc The tale of tail-anchored proteins: coming from the cytosol and looking for a membrane
    Nica Borgese
    Consiglio Nazionale delle Ricerche Institute for Neuroscience, Cellular and Molecular Pharmacology Section, Via Vanvitelli 32, 20129 Milano, Italy
    J Cell Biol 161:1013-9. 2003
    ....
  4. pmc Transmembrane topogenesis of a tail-anchored protein is modulated by membrane lipid composition
    Silvia Brambillasca
    CNR Institute of Neuroscience Cell Mol Pharmacology and Department of Medical Pharmacology, University of Milan, Milan, Italy
    EMBO J 24:2533-42. 2005
    ..These results identify the minimal requirements for transmembrane topogenesis of a TA protein and suggest that selectivity among various intracellular compartments can be imparted by differences in their lipid composition...
  5. ncbi request reprint How tails guide tail-anchored proteins to their destinations
    Nica Borgese
    National Research Council Institute for Neuroscience and Department of Medical Pharmacology, University of Milan, Via Vanvitelli 32, 20129 Milano, Italy
    Curr Opin Cell Biol 19:368-75. 2007
    ..Unravelling these pathways will shed light on the biosynthesis and regulation of an important group of membrane proteins and is likely to lead to new concepts in the field of membrane biogenesis...
  6. doi request reprint Targeting pathways of C-tail-anchored proteins
    Nica Borgese
    National Research Council Institute for Neuroscience and Department of Medical Pharmacology, University of Milan, Milano, Italy
    Biochim Biophys Acta 1808:937-46. 2011
    ..This article is part of a Special Issue entitled Protein translocation across or insertion into membranes...
  7. ncbi request reprint Biogenesis of tail-anchored proteins
    N Borgese
    Consiglio Nazionale delle Ricerche Institute for Neuroscience, Cellular and Molecular Pharmacology Section, University of Milan, Via Vanvitelli 32, 20129 Milan, Italy
    Biochem Soc Trans 31:1238-42. 2003
    ..Here we summarize recent results on the insertion of tail-anchored proteins and discuss possible mechanisms that could be involved...
  8. doi request reprint Remote origins of tail-anchored proteins
    Nica Borgese
    Consiglio Nazionale delle Ricerche Institute of Neuroscience and Department of Pharmacology, Universita degli Studi di Milano, Via Vanvitelli 32, 20129 Milano, Italy
    Traffic 11:877-85. 2010
    ..Thus, eukaryotes may have inherited the GET3 targeting pathway from our archeal ancestor, while the bacterial homologue may be exclusively dedicated to heavy metal resistance...
  9. ncbi request reprint Endoplasmic reticulum architecture: structures in flux
    Nica Borgese
    National Research Council Institute for Neuroscience and Department of Medical Pharmacology, University of Milan, Via Vanvitelli 32, 20129 Milano, Italy
    Curr Opin Cell Biol 18:358-64. 2006
    ..The mechanisms that modulate ER structure are likely to be important for the generation of the characteristic shapes of other organelles...
  10. pmc Targeting of a tail-anchored protein to endoplasmic reticulum and mitochondrial outer membrane by independent but competing pathways
    N Borgese
    Consiglio Nazionale delle Ricerche, Cellular and Molecular Pharmacology Center and Department of Medical Pharmacology, University of Milan, Milan, Italy
    Mol Biol Cell 12:2482-96. 2001
    ..Thus, targeting occurs directly from the cytosol. Moreover, ER and MOM compete for the same polypeptide, explaining the dual localization of some TA proteins...
  11. pmc A role for N-myristoylation in protein targeting: NADH-cytochrome b5 reductase requires myristic acid for association with outer mitochondrial but not ER membranes
    N Borgese
    Consiglio Nazionale delle Ricerche Cellular and Molecular Pharmacology Center, Department of Pharmacology, University of Milan, Italy
    J Cell Biol 135:1501-13. 1996
    ..Our results indicate that myristoylated reductase localizes to ER and mitochondria by different mechanisms, and reveal a novel role for myristic acid in protein targeting...
  12. ncbi request reprint An erythroid-specific transcript generates the soluble form of NADH-cytochrome b5 reductase in humans
    A Bulbarelli
    Consiglio Nazionale delle Ricerche Center for Cellular and Molecular Pharmacology, Department of Pharmacology, University of Milan, Milan, Italy
    Blood 92:310-9. 1998
    ..Our results indicate that the S-transcript is expressed at late stages of erythroid maturation to generate soluble b5R...
  13. pmc Transmembrane domain-dependent partitioning of membrane proteins within the endoplasmic reticulum
    Paolo Ronchi
    Consiglio Nazionale delle Ricerche, Institute of Neuroscience, Cellular and Molecular Pharmacology, University of Milan, 20129 Milan, Italy
    J Cell Biol 181:105-18. 2008
    ..Thus, physicochemical features of the TMD influence sorting of membrane proteins both within the ER and at the ER-Golgi boundary by simple receptor-independent mechanisms based on partitioning...
  14. ncbi request reprint The targeting information of the mitochondrial outer membrane isoform of cytochrome b5 is contained within the carboxyl-terminal region
    M De Silvestris
    C N R Center for Cytopharmacology, Milano, Italy
    FEBS Lett 370:69-74. 1995
    ..The chimera localized to mitochondria, indicating that the carboxyl-terminal 43 amino acids of OM b5 contain sufficient information to target the catalytic domain of ER b5 to the mitochondrial outer membrane...
  15. ncbi request reprint Dynamic and reversible restructuring of the ER induced by PDMP in cultured cells
    Teresa Sprocati
    Consiglio Nazionale delle Ricerche Institute of Neuroscience and Department of Medical Pharmacology, Via Vanvitelli 32, University of Milano, 20129 Milano, Italy
    J Cell Sci 119:3249-60. 2006
    ..Our results demonstrate a dynamic relationship between smooth and rough ER and have implications for the mechanisms regulating ER architecture...
  16. pmc Unassisted translocation of large polypeptide domains across phospholipid bilayers
    Silvia Brambillasca
    Cellular and Molecular Pharmacology Section, Consiglio Nazionale delle Ricerche Institute of Neuroscience, University of Milan, 20129 Milan, Italy
    J Cell Biol 175:767-77. 2006
    ..Our data resolve long-standing discrepancies on TA protein insertion and are relevant to membrane evolution, biogenesis, and physiology...
  17. doi request reprint The role of cytosolic proteins in the insertion of tail-anchored proteins into phospholipid bilayers
    Sara F Colombo
    CNR Institute for Neuroscience and Department of Pharmacology, Universita degli Studi di Milano, Italy
    J Cell Sci 122:2383-92. 2009
    ..The effect of diamide is also observed in living cells. Thus, the specific in vivo targeting of b5 might be achieved by interaction with redox-sensitive targeting factors that hinder its nonspecific insertion into any permissive bilayer...
  18. pmc N-myristoylation determines dual targeting of mammalian NADH-cytochrome b5 reductase to ER and mitochondrial outer membranes by a mechanism of kinetic partitioning
    Sara Colombo
    Consiglio Nazionale delle Ricerche Institute of Neuroscience, Cellular and Molecular Pharmacology Section and Department of Medical Pharmacology, University of Milan, 20129 Milan, Italy
    J Cell Biol 168:735-45. 2005
    ..Thus, competition between two cotranslational events, binding of signal recognition particle and modification by N-myristoylation, determines the site of translation and the localization of b5R...
  19. doi request reprint A VAPB mutant linked to amyotrophic lateral sclerosis generates a novel form of organized smooth endoplasmic reticulum
    Elisa Fasana
    Consiglio Nazionale delle Ricerche Institute of Neuroscience, Via Vanvitelli 32, 20129 Milano, Italy
    FASEB J 24:1419-30. 2010
    ..Our results demonstrate that the ALS-linked VAPB mutant causes dramatic ER restructuring that may underlie its pathogenicity in motoneurons...
  20. ncbi request reprint Translocation of the C terminus of a tail-anchored protein across the endoplasmic reticulum membrane in yeast mutants defective in signal peptide-driven translocation
    Monica Yabal
    Program of Cellular Biotechnology, Institute of Biotechnology, University of Helsinki, Viikinkaari 9, 00710 Helsinki, Finland
    J Biol Chem 278:3489-96. 2003
    ..Thus, translocation of tail-anchored b(5)-Nglyc proceeds by a mechanism different from that of signal peptide-driven post-translational translocation...
  21. pmc Formation of stacked ER cisternae by low affinity protein interactions
    Erik L Snapp
    Cell Biology and Metabolism Branch, National Institutes of Child Health and Human Development, National Institutes of Health, 18 Library Dr, Bldg 18T, Rm 101, Bethesda, MD 20892, USA
    J Cell Biol 163:257-69. 2003
    ..This mechanism may underlie the formation of other stacked membrane structures within cells...
  22. ncbi request reprint Endothelial nitric oxide synthase is segregated from caveolin-1 and localizes to the leading edge of migrating cells
    Stefania Bulotta
    Department of Pharmaco Biological Science, University of Catanzaro Magna Graecia, 88021 Catanzaro, Italy
    Exp Cell Res 312:877-89. 2006
    ..Our results provide a morphological correlate for the role of eNOS in cell migration and raise questions on the site of interaction between eNOS and caveolin-1...
  23. pmc Two tail-anchored protein variants, differing in transmembrane domain length and intracellular sorting, interact differently with lipids
    Paolo Ceppi
    Department of Experimental, Environmental Medicine, and Biotechnologies DIMESAB, Medical School, University of Milano Bicocca, 20052 Monza, Italy
    Proc Natl Acad Sci U S A 102:16269-74. 2005
    ..The dissimilar interaction with lipids of these two differently localized TA proteins could have implications for their intracellular sorting...