I Bertini

Summary

Affiliation: University of Florence
Country: Italy

Publications

  1. ncbi The unfolding of oxidized c-type cytochromes: the instructive case of Bacillus pasteurii
    Ilaria Bartalesi
    Magnetic Resonance Center, University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Italy
    J Mol Biol 321:693-701. 2002
  2. ncbi High-resolution characterization of intrinsic disorder in proteins: expanding the suite of (13)C-detected NMR spectroscopy experiments to determine key observables
    Ivano Bertini
    CERM University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Italy
    Chembiochem 12:2347-52. 2011
  3. ncbi On the use of ultracentrifugal devices for sedimented solute NMR
    Ivano Bertini
    Center for Magnetic Resonance CERM, University of Florence, Via L Sacconi 6, 50019 Sesto Fiorentino, FI, Italy
    J Biomol NMR 54:123-7. 2012
  4. doi MaxOcc: a web portal for maximum occurrence analysis
    Ivano Bertini
    Magnetic Resonance Center CERM, University of Florence, Via L Sacconi 6, 50019 Sesto Fiorentino, FI, Italy
    J Biomol NMR 53:271-80. 2012
  5. doi Paramagnetic relaxation enhancement for the characterization of the conformational heterogeneity in two-domain proteins
    Ivano Bertini
    Magnetic Resonance Center CERM, University of Florence, Via L Sacconi 6, 50019 Sesto Fiorentino FI, Italy
    Phys Chem Chem Phys 14:9149-56. 2012
  6. doi Structural insights into the ferroxidase site of ferritins from higher eukaryotes
    Ivano Bertini
    Magnetic Resonance Center CERM, University of Florence, 50019 Sesto Fiorentino, Florence, Italy
    J Am Chem Soc 134:6169-76. 2012
  7. doi The catalytic domain of MMP-1 studied through tagged lanthanides
    Ivano Bertini
    Magnetic Resonance Center CERM, University of Florence, Via Sacconi 6, 50019 Sesto Fiorentino, Italy
    FEBS Lett 586:557-67. 2012
  8. doi Metabolomic NMR fingerprinting to identify and predict survival of patients with metastatic colorectal cancer
    Ivano Bertini
    CERM and Department of Chemistry, University of Florence, Florence, Italy
    Cancer Res 72:356-64. 2012
  9. doi Accurate solution structures of proteins from X-ray data and a minimal set of NMR data: calmodulin-peptide complexes as examples
    Ivano Bertini
    Magnetic Resonance Center CERM, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Italy
    J Am Chem Soc 131:5134-44. 2009
  10. doi Solution structure and dynamics of S100A5 in the apo and Ca2+-bound states
    Ivano Bertini
    Magnetic Resonance Center CERM, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Italy
    J Biol Inorg Chem 14:1097-107. 2009

Detail Information

Publications142 found, 100 shown here

  1. ncbi The unfolding of oxidized c-type cytochromes: the instructive case of Bacillus pasteurii
    Ilaria Bartalesi
    Magnetic Resonance Center, University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Italy
    J Mol Biol 321:693-701. 2002
    ..It is concluded that the thermodynamic stability of the region around the metal cofactor is determined by the chemical nature of the residues around the axial methionine residue...
  2. ncbi High-resolution characterization of intrinsic disorder in proteins: expanding the suite of (13)C-detected NMR spectroscopy experiments to determine key observables
    Ivano Bertini
    CERM University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Italy
    Chembiochem 12:2347-52. 2011
    ..The test was performed on the yeast Cox17 protein, known to gain its function through maturation from an intrinsically disordered state (see figure)...
  3. ncbi On the use of ultracentrifugal devices for sedimented solute NMR
    Ivano Bertini
    Center for Magnetic Resonance CERM, University of Florence, Via L Sacconi 6, 50019 Sesto Fiorentino, FI, Italy
    J Biomol NMR 54:123-7. 2012
    ..We designed two different devices and we here report the directions for using such devices and the relevant equations for determining the parameters for sedimentation...
  4. doi MaxOcc: a web portal for maximum occurrence analysis
    Ivano Bertini
    Magnetic Resonance Center CERM, University of Florence, Via L Sacconi 6, 50019 Sesto Fiorentino, FI, Italy
    J Biomol NMR 53:271-80. 2012
    ..cerm.unifi.it/access/index/maxocc . It can be used freely upon registration to the grid with a digital certificate...
  5. doi Paramagnetic relaxation enhancement for the characterization of the conformational heterogeneity in two-domain proteins
    Ivano Bertini
    Magnetic Resonance Center CERM, University of Florence, Via L Sacconi 6, 50019 Sesto Fiorentino FI, Italy
    Phys Chem Chem Phys 14:9149-56. 2012
    ..The data, acquired at 298 K and at 278 K, suggest that compact conformations are disfavoured by decreasing the temperature...
  6. doi Structural insights into the ferroxidase site of ferritins from higher eukaryotes
    Ivano Bertini
    Magnetic Resonance Center CERM, University of Florence, 50019 Sesto Fiorentino, Florence, Italy
    J Am Chem Soc 134:6169-76. 2012
    ..Here two different di-iron species are trapped in the active site, with intermetal distances corresponding to those of the ferric dimer in crystal 1 and of the dicopper centers and corresponding rearrangement of the His54 side chain...
  7. doi The catalytic domain of MMP-1 studied through tagged lanthanides
    Ivano Bertini
    Magnetic Resonance Center CERM, University of Florence, Via Sacconi 6, 50019 Sesto Fiorentino, Italy
    FEBS Lett 586:557-67. 2012
    ....
  8. doi Metabolomic NMR fingerprinting to identify and predict survival of patients with metastatic colorectal cancer
    Ivano Bertini
    CERM and Department of Chemistry, University of Florence, Florence, Italy
    Cancer Res 72:356-64. 2012
    ..Our findings establish that (1)H-NMR profiling of patient serum can provide a strong metabolomic signature of mCRC and that analysis of this signature may offer an independent tool to predict OS...
  9. doi Accurate solution structures of proteins from X-ray data and a minimal set of NMR data: calmodulin-peptide complexes as examples
    Ivano Bertini
    Magnetic Resonance Center CERM, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Italy
    J Am Chem Soc 131:5134-44. 2009
    ....
  10. doi Solution structure and dynamics of S100A5 in the apo and Ca2+-bound states
    Ivano Bertini
    Magnetic Resonance Center CERM, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Italy
    J Biol Inorg Chem 14:1097-107. 2009
    ....
  11. pmc Interdomain flexibility in full-length matrix metalloproteinase-1 (MMP-1)
    Ivano Bertini
    Magnetic Resonance Center, University of Florence, Sesto Fiorentino, Italy
    J Biol Chem 284:12821-8. 2009
    ....
  12. ncbi Perspectives in paramagnetic NMR of metalloproteins
    Ivano Bertini
    Magnetic Resonance Center, University of Florence, Via L Sacconi 6, 50019, Sesto Fiorentino, FI, Italy
    Dalton Trans . 2008
    ....
  13. ncbi Intra- and interdomain flexibility in matrix metalloproteinases: functional aspects and drug design
    Ivano Bertini
    Magnetic Resonance Center CERM, University of Florence, Via L Sacconi 6, 50019 Sesto Fiorentino, Italy
    Curr Pharm Des 15:3592-605. 2009
    ..The implications of the observed intra- and interdomain flexibility in matrix metalloproteinases for drug design have been analyzed and discussed...
  14. doi Ultrafast MAS solid-state NMR permits extensive 13C and 1H detection in paramagnetic metalloproteins
    Ivano Bertini
    Magnetic Resonance Center, CERM, University of Florence, Sesto Fiorentino, Italy
    J Am Chem Soc 132:5558-9. 2010
    ..Furthermore, using the known crystal structure of the compound, we are able to distinguish and measure pseudocontact (PCS) contributions to the shifts up to the coordinating ligands and to unveil structural information...
  15. doi NMR properties of sedimented solutes
    Ivano Bertini
    Magnetic Resonance Center CERM, University of Florence, Sesto Fiorentino FI, Italy
    Phys Chem Chem Phys 14:439-47. 2012
    ..The latter possibility opens new horizons for NMR of sedimented systems...
  16. doi Conformational space of flexible biological macromolecules from average data
    Ivano Bertini
    CERM, University of Florence, Via L Sacconi 6, 50019 Sesto Fiorentino, Italy
    J Am Chem Soc 132:13553-8. 2010
    ..The computer program is publicly available using the grid computing infrastructure through the authors' Web portal...
  17. pmc Solid-state NMR of proteins sedimented by ultracentrifugation
    Ivano Bertini
    Magnetic Resonance Center CERM and Department of Chemistry, University of Florence, Via L Sacconi 6, 50019 Sesto Fiorentino, Italy
    Proc Natl Acad Sci U S A 108:10396-9. 2011
    ..NMR of transiently sedimented molecules under fast magic angle spinning has the advantage of overcoming protein size limitations of solution NMR without the need of sample crystallization/precipitation required by solid-state NMR...
  18. doi A new structural model of Aβ40 fibrils
    Ivano Bertini
    Magnetic Resonance Center CERM, University of Florence, Via L Sacconi 6, 50019 Sesto Fiorentino, Italy
    J Am Chem Soc 133:16013-22. 2011
    ..The revealed structural diversity in Aβ fibrils points to a complex picture of Aβ fibrillation...
  19. doi A Grid-enabled web portal for NMR structure refinement with AMBER
    Ivano Bertini
    Magnetic Resonance Center CERM, University of Florence, Via L Sacconi 6, Italy
    Bioinformatics 27:2384-90. 2011
    ..This refinement however presents various subtleties, from the proper formatting of conformational restraints to the definition of suitable protocols...
  20. doi 13C direct-detection biomolecular NMR spectroscopy in living cells
    Ivano Bertini
    CERM and Department of Chemistry Ugo Schiff, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Florence, Italy
    Angew Chem Int Ed Engl 50:2339-41. 2011
  21. doi Principles and patterns in the interaction between mono-heme cytochrome c and its partners in electron transfer processes
    Ivano Bertini
    Magnetic Resonance Center, University of Florence, Via L Sacconi 6, 50019 Sesto Fiorentino, Italy
    Metallomics 3:354-62. 2011
    ....
  22. ncbi Menkes disease
    I Bertini
    Magnetic Resonance Center CERM, University of Florence, Via L Sacconi 6, 50019, Sesto Fiorentino, Italy
    Cell Mol Life Sci 65:89-91. 2008
    ..The mechanism for sensing the concentration of copper, for trafficking, as well as the details of the mechanism of copper translocation across the membrane are unknown...
  23. doi Evidence of reciprocal reorientation of the catalytic and hemopexin-like domains of full-length MMP-12
    Ivano Bertini
    Magnetic Resonance Center CERM, University of Florence, Via L Sacconi 6, 50019 Sesto Fiorentino, Italy
    J Am Chem Soc 130:7011-21. 2008
    ..Hints on the probable conformations are also obtained. This experimental finding opens new perspectives for the often hypothesized active role of the hemopexin-like domain in the enzymatic activity of MMPs...
  24. ncbi Mapping protein-protein interaction by 13C'-detected heteronuclear NMR spectroscopy
    Ivano Bertini
    Department of Chemistry, University of Florence, 50019, Sesto Fiorentino, Italy
    J Biomol NMR 36:111-22. 2006
    ..It is here proposed that protonless (13)C direct-detection NMR is a useful complement to (1)H based NMR spectroscopy for monitoring protein-protein and protein-ligand interactions...
  25. ncbi Monomorphism of human cytochrome c
    Ivano Bertini
    Magnetic Resonance Center, University of Florence, 50019 Sesto Fiorentino, Italy
    Genomics 88:669-72. 2006
    ..Surprisingly, none of the putative SNPs survived experimental validation. We conclude that non-rare allelic variants of the Cyt c protein are absent in the human populations analyzed in this study...
  26. ncbi Towards a protocol for solution structure determination of copper(II) proteins: the case of Cu(II)Zn(II) superoxide dismutase
    Ivano Bertini
    CERM and Department of Chemistry, University of Florence, Via L Sacconi 6, 50019 Sesto Fiorentino, Italy
    Chembiochem 8:1422-9. 2007
    ..1H NOEs and 1H and 13C R1 data are sufficient to produce a good/reasonable solution structure, as demonstrated for a monomeric species of superoxide dismutase, a 153-residue protein...
  27. ncbi Protonless 13C direct detection NMR: characterization of the 37 kDa trimeric protein CutA1
    Ivano Bertini
    Magnetic Resonance Center, University of Florence, Sesto Fiorentino, Italy
    Proteins 70:1196-205. 2008
    ..The structural and dynamical knowledge obtained for this system may contribute to understand its biological role...
  28. ncbi Evolution of mitochondrial-type cytochrome c domains and of the protein machinery for their assembly
    Ivano Bertini
    Magnetic Resonance Center CERM, University of Florence, Via L Sacconi 6, 50019 Sesto Fiorentino, Italy
    J Inorg Biochem 101:1798-811. 2007
    ..Archaea have inherited from Bacteria system I or, possibly, an evolutionary intermediate between system II and system I...
  29. ncbi Snapshots of the reaction mechanism of matrix metalloproteinases
    Ivano Bertini
    Magnetic Resonance Center CERM, University of Florence, Via L Sacconi 6, 50019 Sesto Fiorentino, Italy
    Angew Chem Int Ed Engl 45:7952-5. 2006
  30. ncbi Asymmetry in 13C-13C COSY spectra provides information on ligand geometry in paramagnetic proteins
    Ivano Bertini
    Magnetic Resonance Center, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Florence, Italy
    J Am Chem Soc 127:12216-7. 2005
    ..The monodentate or bidentate metal binding mode of carboxylates was identified directly via NMR...
  31. ncbi NMR spectroscopy of paramagnetic metalloproteins
    Ivano Bertini
    Magnetic Resonance Center, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Italy
    Chembiochem 6:1536-49. 2005
    ..Finally, a section is dedicated to the significant progress made on 13C direct detection, which reduces the negative effects of paramagnetism and may constitute a new chapter in the whole field of NMR spectroscopy...
  32. ncbi Paramagnetic metal ions in ligand screening: the Co(II) matrix metalloproteinase 12
    Ivano Bertini
    Magnetic Resonance Center, University of Florence, Via L Sacconi 6, 50019 Sesto Fiorentino, Italy
    Angew Chem Int Ed Engl 43:2254-6. 2004
  33. ncbi Exploring the subtleties of drug-receptor interactions: the case of matrix metalloproteinases
    Ivano Bertini
    Magnetic Resonance Center CERM, University of Florence, Via L Sacconi 6, 50019 Sesto Fiorentino, Italy
    J Am Chem Soc 129:2466-75. 2007
    ....
  34. ncbi Paramagnetism-based NMR restraints provide maximum allowed probabilities for the different conformations of partially independent protein domains
    Ivano Bertini
    Magnetic Resonance Center CERM, University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Italy
    J Am Chem Soc 129:12786-94. 2007
    ..Such variety is somewhat reduced in the calmodulin-alpha-synuclein adduct, which however still retains high flexibility. The flexibility of the calmodulin-alpha-synuclein adduct is an unexpected result of this research...
  35. ncbi Metals in the "omics" world: copper homeostasis and cytochrome c oxidase assembly in a new light
    Ivano Bertini
    Magnetic Resonance Center CERM, University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Italy
    J Biol Inorg Chem 13:3-14. 2008
    ..The long-term goal of this approach is the overall description of how metals are framed as essential factors within living cells, which in fact is the ultimate purpose of bioinorganic chemistry...
  36. ncbi Solution structure of the Cu(I) and apo forms of the yeast metallochaperone, Atx1
    F Arnesano
    Magnetic Resonance Center CERM, University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Florence, Italy
    Biochemistry 40:1528-39. 2001
    ..L., Hou, M. Y., Wernimont, A. K., Pufahl, R. A., and O'Halloran, T. V. (1999) Structure 7, 605-617] provides insights into the copper transfer mechanism, and a pivotal role for Lys65 in the metal capture and release process is proposed...
  37. ncbi Copper trafficking: the solution structure of Bacillus subtilis CopZ
    L Banci
    Centro di Risonanze Magnetiche and Department of Chemistry, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Italy
    Biochemistry 40:15660-8. 2001
    ..The nature and the location of conserved residues around the metal-binding site are discussed with respect to their relevance for the metal-binding process. Proposals for the role of CopZ are also presented...
  38. ncbi Backbone dynamics of plastocyanin in both oxidation states. Solution structure of the reduced form and comparison with the oxidized state
    I Bertini
    Magnetic Resonance Center and Department of Chemistry, University of Florence, Via L Sacconi, 6 50019 Sesto Fiorentino, Italy
    J Biol Chem 276:47217-26. 2001
    ..The present characterization provides information on both the structural and dynamic behavior of blue copper proteins in solution that is useful to understand further the role(s) of protein dynamics in electron transfer processes...
  39. ncbi Paramagnetism-based versus classical constraints: an analysis of the solution structure of Ca Ln calbindin D9k
    I Bertini
    Magnetic Resonance Center CERM and Department of Chemistry, University of Florence, Sesto Fiorentino, Italy
    J Biomol NMR 21:85-98. 2001
    ....
  40. ncbi The three-dimensional solution structure of the reduced high-potential iron-sulfur protein from Chromatium vinosum through NMR
    L Banci
    Department of Chemistry, University of Florence, Italy
    Biochemistry 34:206-19. 1995
    ..These values compare well with those for diamagnetic proteins of the same size. The solution structure is discussed in the light of the available structural information from X-ray data...
  41. ncbi Lanthanide induced residual dipolar couplings for the conformational investigation of peripheral 15NH2 moieties
    I Bertini
    Magnetic Resonance Center CERM, University of Florence, Italy
    J Biomol NMR 18:347-55. 2000
    ..The exploitation of auto-orientation of magnetic anisotropic metalloproteins represents a step ahead in the investigation of the conformational space of peripheral residues that are not fixed by the protein folding...
  42. ncbi Three-dimensional structure of the reduced C77S mutant of the Chromatium vinosum high-potential iron-sulfur protein through nuclear magnetic resonance: comparison with the solution structure of the wild-type protein
    D Bentrop
    Department of Chemistry, University of Florence, Italy
    Biochemistry 35:5928-36. 1996
    ..Capillary electrophoresis experiments demonstrate coordination of Ser-77 as an anion. Serine versus cysteine coordination in iron-sulfur proteins is briefly discussed...
  43. ncbi (15)N backbone dynamics of ferricytochrome b(562): comparison with the reduced protein and the R98C variant
    M Assfalg
    Magnetic Resonance Center and Department of Chemistry, University of Florence, Sesto Fiorentino, Italy
    Biochemistry 40:12761-71. 2001
    ..It appears that the increased protein stability of the variant, established previously, is not correlated with an increase in rigidity...
  44. ncbi Solution structure of the yeast copper transporter domain Ccc2a in the apo and Cu(I)-loaded states
    L Banci
    Magnetic Resonance Center and Department of Chemistry, University of Florence, Via Luigi Sacconi 6, Sesto Fiorentino, Florence, 50019, Italy
    J Biol Chem 276:8415-26. 2001
    ..These results open new mechanistic aspects of copper transporter domains with physiological copper donor and acceptor proteins...
  45. ncbi Heme methyl 1H chemical shifts as structural parameters in some low-spin ferriheme proteins
    I Bertini
    Center for Magnetic Resonance, Department of Chemistry, University of Florence, Italy
    J Biol Inorg Chem 4:515-9. 1999
    ..Therefore, by taking advantage of this study, information on the position of the axial ligands, that can be used as a constraint for structure determination, can be obtained from the shifts of the methyl protons...
  46. ncbi ePHOGSY experiments on a paramagnetic protein: location of the catalytic water molecule in the heme crevice of the oxidized form of horse heart cytochrome c
    I Bertini
    Department of Chemistry, University of Florence, Italy
    FEBS Lett 415:45-8. 1997
    ..The resulting picture is that there is a detectable movement of the water molecule upon oxidation...
  47. ncbi Three-dimensional solution structure of the oxidized high potential iron-sulfur protein from Chromatium vinosum through NMR. Comparative analysis with the solution structure of the reduced species
    I Bertini
    Department of Chemistry, University of Florence, Italy
    Biochemistry 34:9851-8. 1995
    ..This is the first time that independently determined solution structures of two redox states of a paramagnetic protein are available. Differences between them and the X-ray structure are discussed...
  48. ncbi The solution structure of oxidized Escherichia coli cytochrome b562
    F Arnesano
    Department of Chemistry, Centro di Risonanze Magnetiche, University of Florence, Italy
    Biochemistry 38:8657-70. 1999
    ..Quite intriguing is the comparison of the structure of cytochrome b562 with the available structures of cytochromes c' which display a similar folding motif and similar pKa values but very little sequence similarity...
  49. ncbi Solution structure and backbone dynamics of the Cu(I) and apo forms of the second metal-binding domain of the Menkes protein ATP7A
    Lucia Banci
    Magnetic Resonance Center, University of Florence, Via L Sacconi 6, 50019 Sesto Fiorentino, Italy
    Biochemistry 43:3396-403. 2004
    ..A comparison with metallochaperones and soluble domains of P-type ATPases allows us to relate the primary structure to the occurrence of structural rearrangements upon copper binding...
  50. ncbi Solution structure of Sco1: a thioredoxin-like protein Involved in cytochrome c oxidase assembly
    Erica Balatri
    Magnetic Resonance Center CERM and Department of Chemistry, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Florence, Italy
    Structure 11:1431-43. 2003
    ..The availability of the structure has allowed us to model the homologs Sco1 and Sco2 from S. cerevisiae and to discuss the physiological role of the Sco family...
  51. ncbi Solution structure of reduced microsomal rat cytochrome b5
    L Banci
    Department of Chemistry, University of Florence, Italy
    Eur J Biochem 249:270-9. 1997
    ..The structural features in solution of the residues proposed to be involved in protein-protein recognition are found to be largely conserved with respect to the solid state...
  52. ncbi Solution structure calculations through self-orientation in a magnetic field of a cerium(III) substituted calcium-binding protein
    I Bertini
    Department of Chemistry, University of Florence, Via Gino Capponi 9, Florence, 50121, Italy
    J Magn Reson 148:23-30. 2001
    ..This paper shows that Ce(III), like low-spin Fe(III) in hemoproteins, is sufficiently magnetically anisotropic to induce self-orientation to an extent which can be exploited for solution structure determination...
  53. ncbi Solution structure of reduced monomeric Q133M2 copper, zinc superoxide dismutase (SOD). Why is SOD a dimeric enzyme?
    L Banci
    Department of Chemistry, University of Florence, Italy
    Biochemistry 37:11780-91. 1998
    ..The hydrogen atom which binds the histidine nitrogen detached from copper(I) is structurally identified...
  54. ncbi The solution structure of oxidized rat microsomal cytochrome b5
    F Arnesano
    Department of Chemistry, University of Florence, Italy
    Biochemistry 37:173-84. 1998
    ..This observation could suggest that, to optimize the electron transfer process, the local mobility should be properly tuned...
  55. ncbi Solution structure of oxidized microsomal rabbit cytochrome b5. Factors determining the heterogeneous binding of the heme
    L Banci
    Department of Chemistry and Centro di Risonanze Magnetiche, University of Florence, Sesto Fiorentino, Italy
    Eur J Biochem 267:755-66. 2000
    ..Hydrophobic and steric interactions are the key factors in determining the relative stability of one isomer with respect to the other...
  56. ncbi Side chain mobility as monitored by CH-CH cross correlation: the example of cytochrome b5
    L Banci
    Magnetic Resonance Center (CERM, University of Florence, Sesto Fiorentino, Italy
    J Biomol NMR 20:1-10. 2001
    ..The general pattern is the same for the oxidized and reduced species, indicating that any oxidation-dependent property detected for backbone NH moieties does not affect the CH2 mobility...
  57. ncbi Characterization of a partially unfolded high potential iron protein
    I Bertini
    Department of Chemistry, University of Florence, via Gino Capponi, 7, 50121 Florence, Italy
    Biochemistry 36:9332-9. 1997
    ....
  58. ncbi Solution structure of apo CopZ from Bacillus subtilis: further analysis of the changes associated with the presence of copper
    Lucia Banci
    Department of Chemistry and Centro di Risonanze Magnetiche, University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Italy
    Biochemistry 42:13422-8. 2003
    ..It is also shown that copper(I) exchanges among apo CopZ molecules in slow exchange on the NMR time scale, whereas it is known that such exchange between partner molecules (i.e., metallochaperones and metal pumps) is fast...
  59. ncbi The evolutionarily conserved trimeric structure of CutA1 proteins suggests a role in signal transduction
    Fabio Arnesano
    Magnetic Resonance Center CERM and Department of Chemistry, University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Florence, Italy
    J Biol Chem 278:45999-6006. 2003
    ..This similarity suggests an intriguing role of CutA1 proteins in signal transduction through allosteric communications between subunits...
  60. ncbi Solution structure of Apo Cu,Zn superoxide dismutase: role of metal ions in protein folding
    Lucia Banci
    Department of Chemistry and Centro Risonanze Magnetiche, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Italy
    Biochemistry 42:9543-53. 2003
    ....
  61. ncbi Protein stability and mutations in the axial methionine loop of a minimal cytochrome c
    Ilaria Bartalesi
    Magnetic Resonance Center, University of Florence, Via L Sacconi 6, 50019 Sesto Fiorentino, Italy
    J Biol Inorg Chem 9:600-8. 2004
    ..While the structure remains essentially the same, the stability decreases with mutations. The comparison with mitochondrial c-type cytochromes is instructive...
  62. ncbi Solution structure of Cox11, a novel type of beta-immunoglobulin-like fold involved in CuB site formation of cytochrome c oxidase
    Lucia Banci
    Magnetic Resonance Center CERM and Department of Chemistry, University of Florence, Via Luigi Sacconi 6, Sesto Fiorentino 50019, Florence, Italy
    J Biol Chem 279:34833-9. 2004
    ..The present results advance the knowledge on the poorly understood molecular aspects of cytochrome c oxidase assembly...
  63. ncbi A new zinc-protein coordination site in intracellular metal trafficking: solution structure of the Apo and Zn(II) forms of ZntA(46-118)
    Lucia Banci
    Magnetic Resonance Center CERM and Department of Chemistry, University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Italy
    J Mol Biol 323:883-97. 2002
    ....
  64. ncbi Fully metallated S134N Cu,Zn-superoxide dismutase displays abnormal mobility and intermolecular contacts in solution
    Lucia Banci
    Magnetic Resonance Center, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Italy
    J Biol Chem 280:35815-21. 2005
    ..We have here also established that the two free cysteines (6 and 111) do not play a role in this behavior...
  65. ncbi A NMR study of the interaction of a three-domain construct of ATP7A with copper(I) and copper(I)-HAH1: the interplay of domains
    Lucia Banci
    Magnetic Resonance Center CERM, University of Florence, Via L Sacconi 6, 50019 Sesto Fiorentino, Italy
    J Biol Chem 280:38259-63. 2005
    ..This dependence could constitute the molecular mechanism to trigger copper(I) translocation and/or ATP7A relocalization from the trans-Golgi network to the plasmatic membrane...
  66. pmc 15N-1H Residual dipolar coupling analysis of native and alkaline-K79A Saccharomyces cerevisiae cytochrome c
    Michael Assfalg
    Magnetic Resonance Center CERM, University of Florence, 50019 Sesto Fiorentino, Florence, Italy
    Biophys J 84:3917-23. 2003
    ..Importantly, this is the first time that mobility has been found through comparison of RDCs with pseudocontact shifts...
  67. ncbi The crystal structure of the monomeric human SOD mutant F50E/G51E/E133Q at atomic resolution. The enzyme mechanism revisited
    M Ferraroni
    Department of Chemistry, University of Florence, Via Gino Capponi 9, Florence, I 50121, Italy
    J Mol Biol 288:413-26. 1999
    ..These properties of the structure have allowed us to revisit the enzymatic mechanism...
  68. ncbi Solution structure of the oxidized 2[4Fe-4S] ferredoxin from Clostridium pasteurianum
    I Bertini
    Department of Chemistry, University of Florence, Italy
    Eur J Biochem 232:192-205. 1995
    ..pasteurianum ferredoxin family is 78 pm (residues 3-53). Discrepancies in residues 26-28 may arise from the disorder observed in the X-ray structure in that region...
  69. ncbi Structural interplay between calcium(II) and copper(II) binding to S100A13 protein
    Fabio Arnesano
    Magnetic Resonance Center CERM and Department of Chemistry, University of Florence, Via L Sacconi 6, 50019 Sesto Fiorentino, Italy
    Angew Chem Int Ed Engl 44:6341-4. 2005
  70. ncbi Human SOD1 before harboring the catalytic metal: solution structure of copper-depleted, disulfide-reduced form
    Lucia Banci
    Magnetic Resonance Center, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Florence, Italy
    J Biol Chem 281:2333-7. 2006
    ..The structure allows us to further discuss the copper loading mechanism in SOD1...
  71. ncbi Paramagnetism-based refinement strategy for the solution structure of human alpha-parvalbumin
    Irfan Baig
    Magnetic Resonance Centre and Department of Chemistry, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Italy
    Biochemistry 43:5562-73. 2004
    ..0 A resolution. All differences are related to local changes in the amino acidic composition...
  72. ncbi Ortholog search of proteins involved in copper delivery to cytochrome C oxidase and functional analysis of paralogs and gene neighbors by genomic context
    Fabio Arnesano
    Magnetic Resonance Center CERM and Department of Chemistry, University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Florence, Italy
    J Proteome Res 4:63-70. 2005
    ..By linking the assembly system to the copper uptake system, Sco allows COX to face alternative copper trafficking pathways...
  73. ncbi A 15N NMR mobility study on the dicalcium P43M calbindin D9k and its mono-La3+-substituted form
    Ivano Bertini
    Magnetic Resonance Center CERM and Department of Chemistry, University of Florence, Sesto Fiorentino, Italy
    Biochemistry 41:5104-11. 2002
    ..They also demonstrate that substitution of a lanthanide ion for calcium, which is a common procedure, does not significantly alter the mobility of the native protein...
  74. ncbi Solution structure of a monoheme ferrocytochrome c from Shewanella putrefaciens and structural analysis of sequence-similar proteins: functional implications
    Ilaria Bartalesi
    Centro di Risonanze Magnetiche, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Italy
    Biochemistry 41:5112-9. 2002
    ..The structures of the others have been modeled by using the available templates and internally validated. Structural similarities in terms of surface properties account for their biophysical features and provide hints about the function...
  75. ncbi Solution structure of the N-terminal domain of a potential copper-translocating P-type ATPase from Bacillus subtilis in the apo and Cu(I) loaded states
    Lucia Banci
    Magnetic Resonance Center CERM and Department of Chemistry, University of Florence, Via Luigi Sacconi 6, Florence, Sesto Fiorentino, 50019, Italy
    J Mol Biol 317:415-29. 2002
    ....
  76. ncbi Structure and dynamics of reduced Bacillus pasteurii cytochrome c: oxidation state dependent properties and implications for electron transfer processes
    Ilaria Bartalesi
    Centro di Risonanze Magnetiche, University of Florence, Sesto Fiorentino, Italy
    Biochemistry 42:739-45. 2003
    ..By comparing the redox-state dependence of the structural/dynamic properties of Fe-S proteins, cytochrome c, and blue copper proteins, hints are provided for a better comprehension of the electron transfer processes...
  77. ncbi Dynamic properties of the G93A mutant of copper-zinc superoxide dismutase as detected by NMR spectroscopy: implications for the pathology of familial amyotrophic lateral sclerosis
    Eric L Shipp
    Magnetic Resonance Center CERM, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Italy
    Biochemistry 42:1890-9. 2003
    ..Alternatively, a motor neuron-specific substrate may bind this region of the protein, leading to deleterious modifications and/or reactions...
  78. ncbi Understanding copper trafficking in bacteria: interaction between the copper transport protein CopZ and the N-terminal domain of the copper ATPase CopA from Bacillus subtilis
    Lucia Banci
    Department of Chemistry, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino Florence, Italy
    Biochemistry 42:1939-49. 2003
    ..Comparing functional data of homologous proteins of other bacteria, it can be concluded that this class of proteins is involved in copper homeostasis but the specific roles are species dependent...
  79. ncbi Magnetic susceptibility tensor anisotropies for a lanthanide ion series in a fixed protein matrix
    I Bertini
    Department of Chemistry, University of Florence, Via Gino Capponi 9, 50121, Florence, Italy
    J Am Chem Soc 123:4181-8. 2001
    ..A knowledge of magnetic susceptibility anisotropy properties of lanthanides is essential in determining the self-orienting properties of lanthanide complexes in solution when immersed in magnetic fields...
  80. ncbi Paramagnetic NMR analysis of the seven-iron ferredoxin from the hyperthermoacidophilic archaeon Desulfurolobus ambivalens reveals structural similarity to other dicluster ferredoxins
    D Bentrop
    Department of Chemistry, University of Florence, Italy
    Eur J Biochem 236:92-9. 1996
    ..The C alpha-C beta-S-Fe dihedral angles of the cysteine residues coordinating the four-iron cluster could be estimated, and the electronic structure of the three-iron cluster is discussed...
  81. ncbi Characterization of the binding interface between the copper chaperone Atx1 and the first cytosolic domain of Ccc2 ATPase
    F Arnesano
    Magnetic Resonance Center CERM and Department of Chemistry, University of Florence, Via Luigi Sacconi 6, Sesto Fiorentino, Florence 50019, Italy
    J Biol Chem 276:41365-76. 2001
    ..The analogous mobility of Ccc2a in both Cu(I) and apo forms is reduced with respect to Atx1. Such an adduct is relevant as a structural and kinetic model for copper transfer from Atx1 to Ccc2a in physiological conditions...
  82. ncbi 1H NMR studies of Chromatium vinosum cytochrome c'
    I Bertini
    Department of Chemistry, University of Florence, Firenze, Italy
    Arch Biochem Biophys 282:84-90. 1990
    ..Some evidence is provided for conformational flexibilities. Among the oxidized cytochromes c' the present one is capable of binding cyanide, giving rise to a low spin state. The reduced species is a typical high spin iron(II) system...
  83. ncbi A structural-dynamical characterization of human Cox17
    Lucia Banci
    Magnetic Resonance Center Centro Risonanze Magnetiche CERM and Department of Chemistry, University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Florence, Italy
    J Biol Chem 283:7912-20. 2008
    ..The copper(I) form of Cox17(2S-S) has features specific for copper chaperones...
  84. ncbi Metalation of the amyotrophic lateral sclerosis mutant glycine 37 to arginine superoxide dismutase (SOD1) apoprotein restores its structural and dynamical properties in solution to those of metalated wild-type SOD1
    Lucia Banci
    Magnetic Resonance Center, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Italy
    Biochemistry 46:9953-62. 2007
    ..These results suggest further that it is the metal-free apo forms of the mutant SOD1 protein that are the agents of its toxicity...
  85. ncbi A quick solution structure determination of the fully oxidized double mutant K9-10A cytochrome c7 from Desulfuromonas acetoxidans and mechanistic implications
    Michael Assfalg
    Magnetic Resonance Center and Department of Chemistry, University of Florence, Sesto Fiorentino, Italy
    J Biomol NMR 22:107-22. 2002
    ..This finding is in agreement with the identification of the protein area around heme IV as the interacting site...
  86. ncbi Occurrence of copper proteins through the three domains of life: a bioinformatic approach
    Claudia Andreini
    Magnetic Resonance Center CERM and Department of Chemistry, University of Florence, Via L Sacconi 6, 50019 Sesto Fiorentino, Italy
    J Proteome Res 7:209-16. 2008
    ..A network involving proteins having roles in both copper transport and respiration was identified, parts or all of which are detected in the majority of the organisms examined...
  87. ncbi Structural basis for the function of the N-terminal domain of the ATPase CopA from Bacillus subtilis
    Lucia Banci
    Magnetic Resonance Center CERM and Department of Chemistry, University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Florence, Italy
    J Biol Chem 278:50506-13. 2003
    ....
  88. ncbi A hint to search for metalloproteins in gene banks
    Claudia Andreini
    Magnetic Resonance Center CERM, University of Florence, Italy
    Bioinformatics 20:1373-80. 2004
    ..Sometimes, metal binding can be observed in vitro but not be physiologically relevant. At present, there is a lack of specific tools to address the matter of the identification of metalloproteins in databases of gene sequences...
  89. ncbi Superoxide dismutase folding/unfolding pathway: role of the metal ions in modulating structural and dynamical features
    Michael Assfalg
    CERM, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Italy
    J Mol Biol 330:145-58. 2003
    ..4 M, thus demonstrating the importance of metal ions with respect to the unfolding process and protein structure stability. Hints to understand the whole folding process are obtained and discussed...
  90. pmc A prokaryotic superoxide dismutase paralog lacking two Cu ligands: from largely unstructured in solution to ordered in the crystal
    Lucia Banci
    Department of Chemistry, University of Florence, Via Luigi Sacconi 6, 50019 Sesto, Florence, Italy
    Proc Natl Acad Sci U S A 102:7541-6. 2005
    ..Couples of dimers held by hydrophobic interactions and H bonds are further organized in long chains. The order/disorder transition is discussed in terms of metal binding and physical state...
  91. ncbi Protein hydration and location of water molecules in oxidized horse heart cytochrome c by (1)H NMR
    I Bertini
    Department of Chemistry, Magnetic Resonance Center CERM, University of Florence, Via Luigi Sacconi 6, Florence, 50019 Sesto Fiorentino, Italy
    J Magn Reson 147:1-8. 2000
    ..The identification of hydrophilic regions and detection of three long-lived water molecules settles some ambiguities and provides a better representation of the water-protein interactions in oxidized cytochrome c...
  92. ncbi The three-dimensional structure in solution of the paramagnetic high-potential iron-sulfur protein I from Ectothiorhodospira halophila through nuclear magnetic resonance
    L Banci
    Department of Chemistry, University of Florence, Italy
    Eur J Biochem 225:715-25. 1994
    ..We do not know of any previous determination of the structure of a paramagnetic protein in solution by NMR. The effect of paramagnetism on the quality of the structure determination is discussed...
  93. ncbi 15N chemical shift changes in cytochrome b5: redox-dependent vs. guanidinium chloride-induced changes
    I Bertini
    Magnetic Resonance Center, University of Florence, Sesto Fiorentino, Italy
    J Biol Inorg Chem 5:761-4. 2000
    ..It is concluded that changes in hydrogen bonding upon reduction must be modest and cannot account for the observed chemical shift effects. Alternative explanations should thus be looked for...
  94. ncbi NOE and two-dimensional correlated 1H-NMR spectroscopy of cytochrome c' from Chromatium vinosum
    L Banci
    Department of Chemistry, University of Florence, Italy
    Eur J Biochem 204:107-12. 1992
    ..This assignment has rationalized on a sound basis the biochemical behavior of this protein with pH and has showed the utility of this kind of spectroscopy for the other cytochromes c' structures and analogous systems...
  95. ncbi An NMR study of the 7Fe-8S ferredoxin from Rhodopseudomonas palustris and reinterpretation of data on similar systems
    I Bertini
    Department of Chemistry, University of Florence, Italy
    Biochemistry 36:3570-9. 1997
    ..The NMR signal patterns of [3Fe-4S] clusters in both 3Fe-4S and 7Fe-8S ferredoxins have been discussed, and some correlations are proposed between signal patterns and the primary sequence...
  96. ncbi Dimethyl propionate ester heme-containing cytochrome b5: structure and stability
    L Banci
    Magnetic Resonance Center, University of Florence, Italy
    J Biol Inorg Chem 6:490-503. 2001
    ..The available data on the reduction potential and the electron transfer rates are discussed on the basis of the present structural data...
  97. ncbi Paramagnetic relaxation as a tool for solution structure determination: Clostridium pasteurianum ferredoxin as an example
    I Bertini
    Department of Chemistry, University of Florence, Firenze, Italy
    Proteins 29:348-58. 1997
    ....
  98. doi Solution structures of the actuator domain of ATP7A and ATP7B, the Menkes and Wilson disease proteins
    Lucia Banci
    Magnetic Resonance Center CERM University of Florence, Via L Sacconi 6, 50019 Sesto Fiorentino, Italy
    Biochemistry 48:7849-55. 2009
    ....
  99. ncbi NMR structural analysis of cadmium sensing by winged helix repressor CmtR
    Lucia Banci
    Department of Chemistry and Centro Risonanze Magnetiche, University of Florence, Via Luigi Sacconi 6, Florence 50019, Italy
    J Biol Chem 282:30181-8. 2007
    ..Cadmium binding across the dimer interface impairs DNA association by excluding the apo-conformers suited to bind DNA...
  100. ncbi X-ray absorption and NMR spectroscopic studies of CopZ, a copper chaperone in Bacillus subtilis: the coordination properties of the copper ion
    Lucia Banci
    Centro di Risonanze Magnetiche and Department of Chemistry, University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Italy
    Biochemistry 42:2467-74. 2003
    ..These results are instructive and have been discussed with respect to the molecular basis of copper trafficking...
  101. ncbi Folding studies of Cox17 reveal an important interplay of cysteine oxidation and copper binding
    Fabio Arnesano
    Magnetic Resonance Center CERM and Department of Chemistry, University of Florence, Via Luigi Sacconi 6, Sesto Fiorentino, Florence 50019, Italy
    Structure 13:713-22. 2005
    ..This form of the protein is oligomeric. These properties are framed within a complete model of mitochondrial import and COX assembly...