I Bertini

Summary

Affiliation: University of Florence
Country: Italy

Publications

  1. pmc SOD1 and amyotrophic lateral sclerosis: mutations and oligomerization
    Lucia Banci
    Magnetic Resonance Center CERM, Department of Chemistry, University of Florence, Florence, Italy
    PLoS ONE 3:e1677. 2008
  2. pmc In-cell NMR in E. coli to monitor maturation steps of hSOD1
    Lucia Banci
    Magnetic Resonance Center, University of Florence, Sesto Fiorentino, Italy
    PLoS ONE 6:e23561. 2011
  3. pmc Minimal functional sites allow a classification of zinc sites in proteins
    Claudia Andreini
    Magnetic Resonance Center, University of Florence, Sesto Fiorentino, Italy
    PLoS ONE 6:e26325. 2011
  4. pmc Structural basis for matrix metalloproteinase 1-catalyzed collagenolysis
    Ivano Bertini
    Magnetic Resonance Center CERM, University of Florence, Via L Sacconi 6, 50019 Sesto Fiorentino, Italy
    J Am Chem Soc 134:2100-10. 2012
  5. ncbi 13C direct detected experiments: optimization for paramagnetic signals
    Ivano Bertini
    Magnetic Resonance Center, University of Florence, Via L Sacconi 6, 50019 Sesto Fiorentino, Florence, Italy
    J Magn Reson 174:125-32. 2005
  6. ncbi A structural model for the adduct between cytochrome c and cytochrome c oxidase
    Ivano Bertini
    Magnetic Resonance Center, University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Italy
    J Biol Inorg Chem 10:613-24. 2005
  7. ncbi Combining in silico tools and NMR data to validate protein-ligand structural models: application to matrix metalloproteinases
    Ivano Bertini
    Magnetic Resonance Center, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Italy
    J Med Chem 48:7544-59. 2005
  8. ncbi Cytochrome c: occurrence and functions
    Ivano Bertini
    Magnetic Resonance Center CERM, University of Florence, Via L Sacconi 6, 50019 Sesto Fiorentino, Italy
    Chem Rev 106:90-115. 2006
  9. ncbi Efficiency of paramagnetism-based constraints to determine the spatial arrangement of alpha-helical secondary structure elements
    Ivano Bertini
    CERM and Department of Chemistry, University of Florence, Sesto Fiorentino, Italy
    J Biomol NMR 22:123-36. 2002
  10. doi Water-based ligand screening for paramagnetic metalloproteins
    Ivano Bertini
    Magnetic Resonance Center CERM, University of Florence, Via L Sacconi 6, 50019 Sesto Fiorentino, Italy
    Angew Chem Int Ed Engl 47:4533-7. 2008

Detail Information

Publications159 found, 100 shown here

  1. pmc SOD1 and amyotrophic lateral sclerosis: mutations and oligomerization
    Lucia Banci
    Magnetic Resonance Center CERM, Department of Chemistry, University of Florence, Florence, Italy
    PLoS ONE 3:e1677. 2008
    ..The mechanism here proposed for SOD1 mutant oligomerization is absolutely general and it provides a common unique picture for the behaviors of the many SOD1 mutants, of different nature and distributed all over the protein...
  2. pmc In-cell NMR in E. coli to monitor maturation steps of hSOD1
    Lucia Banci
    Magnetic Resonance Center, University of Florence, Sesto Fiorentino, Italy
    PLoS ONE 6:e23561. 2011
    ..However, no intramolecular disulfide bridge formation occurs, nor copper uptake, suggesting the need of a specific chaperone for those purposes...
  3. pmc Minimal functional sites allow a classification of zinc sites in proteins
    Claudia Andreini
    Magnetic Resonance Center, University of Florence, Sesto Fiorentino, Italy
    PLoS ONE 6:e26325. 2011
    ....
  4. pmc Structural basis for matrix metalloproteinase 1-catalyzed collagenolysis
    Ivano Bertini
    Magnetic Resonance Center CERM, University of Florence, Via L Sacconi 6, 50019 Sesto Fiorentino, Italy
    J Am Chem Soc 134:2100-10. 2012
    ..The aforementioned steps provide a detailed, experimentally derived, and energetically favorable collagenolytic mechanism, as well as significant insight into the roles of distinct domains in extracellular protease function...
  5. ncbi 13C direct detected experiments: optimization for paramagnetic signals
    Ivano Bertini
    Magnetic Resonance Center, University of Florence, Via L Sacconi 6, 50019 Sesto Fiorentino, Florence, Italy
    J Magn Reson 174:125-32. 2005
    ....
  6. ncbi A structural model for the adduct between cytochrome c and cytochrome c oxidase
    Ivano Bertini
    Magnetic Resonance Center, University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Italy
    J Biol Inorg Chem 10:613-24. 2005
    ....
  7. ncbi Combining in silico tools and NMR data to validate protein-ligand structural models: application to matrix metalloproteinases
    Ivano Bertini
    Magnetic Resonance Center, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Italy
    J Med Chem 48:7544-59. 2005
    ..This protocol is proposed as a viable alternative to the many approaches described in the literature...
  8. ncbi Cytochrome c: occurrence and functions
    Ivano Bertini
    Magnetic Resonance Center CERM, University of Florence, Via L Sacconi 6, 50019 Sesto Fiorentino, Italy
    Chem Rev 106:90-115. 2006
  9. ncbi Efficiency of paramagnetism-based constraints to determine the spatial arrangement of alpha-helical secondary structure elements
    Ivano Bertini
    CERM and Department of Chemistry, University of Florence, Sesto Fiorentino, Italy
    J Biomol NMR 22:123-36. 2002
    ..In cases like calbindin D9k, the availability of datasets from different metal ions is helpful, whereas less important is the location of the metal ion with respect to the secondary structure elements...
  10. doi Water-based ligand screening for paramagnetic metalloproteins
    Ivano Bertini
    Magnetic Resonance Center CERM, University of Florence, Via L Sacconi 6, 50019 Sesto Fiorentino, Italy
    Angew Chem Int Ed Engl 47:4533-7. 2008
  11. ncbi Locating the metal ion in calcium-binding proteins by using cerium(III) as a probe
    I Bertini
    Magnetic Resonance Center CERM, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Florence, Italy
    Chembiochem 2:550-8. 2001
    ....
  12. doi The metabonomic signature of celiac disease
    Ivano Bertini
    Magnetic Resonance Center CERM, University of Florence, Via L Sacconi 6, 50019 Sesto Fiorentino, Italy
    J Proteome Res 8:170-7. 2009
    ..Altered serum levels of glucose and ketonic bodies suggest alterations of energy metabolism, while the urine data point to alterations of gut microbiota. Metabolomics may thus provide further hints on the biochemistry of the disease...
  13. doi Individual human phenotypes in metabolic space and time
    Patrizia Bernini
    Magnetic Resonance Center CERM, University of Florence, Sesto Fiorentino, Italy
    J Proteome Res 8:4264-71. 2009
    ....
  14. ncbi Paramagnetism-based versus classical constraints: an analysis of the solution structure of Ca Ln calbindin D9k
    I Bertini
    Magnetic Resonance Center CERM and Department of Chemistry, University of Florence, Sesto Fiorentino, Italy
    J Biomol NMR 21:85-98. 2001
    ....
  15. doi Structural basis of serine/threonine phosphatase inhibition by the archetypal small molecules cantharidin and norcantharidin
    I Bertini
    Department of Chemistry, University of Florence, Via della Lastruccia 3, 50019 Sesto Fiorentino, Italy
    J Med Chem 52:4838-43. 2009
    ..All these structures will provide the basis for the rational design of new cantharidin-based drugs...
  16. pmc Application of NMRD to hydration of rubredoxin and a variant containing a (Cys-S)3FeIII(OH) site
    Ivano Bertini
    CERM and Department of Chemistry, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Italy
    Biophys J 84:545-51. 2003
    ..1)H nuclear magnetic relaxation dispersion profiles indicate increased hydration with respect to the wild-type...
  17. ncbi (13)C-(13)C NOESY: a constructive use of (13)C-(13)C spin-diffusion
    Ivano Bertini
    Magnetic Resonance Center and Department of Chemistry, University of Florence, Italy
    J Biomol NMR 30:245-51. 2004
    ..The interpretation was confirmed by simulations. This approach broadens the range of applicability of (13)C-(13)C NOESY spectroscopy...
  18. ncbi NMR spectroscopic detection of protein protons and longitudinal relaxation rates between 0.01 and 50 MHz
    Ivano Bertini
    Magnetic Resonance Center and Department of Chemistry, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Italy
    Angew Chem Int Ed Engl 44:2223-5. 2005
  19. ncbi Cytochrome c and SDS: a molten globule protein with altered axial ligation
    Ivano Bertini
    CERM, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Florence, Italy
    J Mol Biol 336:489-96. 2004
    ..Such findings are meaningful with respect to the physiology of cytochrome c as electron transfer protein and as promoter of apoptosis...
  20. ncbi Crystal structure of the catalytic domain of human matrix metalloproteinase 10
    I Bertini
    CERM, University of Florence and FiorGen Foundation, Via Sacconi 6, 50019 Sesto Fiorentino, Florence, Italy
    J Mol Biol 336:707-16. 2004
    ..Furthermore, the binding mode of N-isobutyl-N-[4-methoxyphenylsulfonyl]glycyl hydroxamic acid (NNGH), which is one of the most known commercial inhibitors of MMPs, is described for the first time...
  21. pmc Experimentally exploring the conformational space sampled by domain reorientation in calmodulin
    Ivano Bertini
    Centre for Magnetic Resonance and Department of Chemistry, University of Florence, Via Luigi Sacconi 6, I 50019 Sesto Fiorentino, Italy
    Proc Natl Acad Sci U S A 101:6841-6. 2004
    ..The same approach is in principle applicable to other multidomain proteins, as well as to multiple interaction modes between two macromolecular partners...
  22. ncbi 13C-13C NOESY: an attractive alternative for studying large macromolecules
    Ivano Bertini
    CERM and Department of Chemistry, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Florence, Italy
    J Am Chem Soc 126:464-5. 2004
    ....
  23. ncbi Thermotoga maritima IscU. Structural characterization and dynamics of a new class of metallochaperone
    Ivano Bertini
    Magnetic Resonance Center, University of Florence, Via L Sacconi, 6 50019 Sesto Fiorentino, Italy
    J Mol Biol 331:907-24. 2003
    ..Additionally, the fluxionality of IscU is unique in that the protein appears to be more compact (based on 1H/2H exchange, R1, R2, and NOE data) but yet more fluid (lack of long-range NOEs) than typical molten globule proteins...
  24. ncbi Tuning the affinity for lanthanides of calcium binding proteins
    Ivano Bertini
    Magnetic Resonance Center and Department of Chemistry, University of Florence, Sesto Fiorentino, Italy
    Biochemistry 42:8011-21. 2003
    ..Finally, by using CaM samples containing only three of the four calcium ions, it was possible to prepare well-defined Ca(3)Ln-CaM derivatives (Ln = Tb, Dy, Tm, and Yb), with interesting properties as NMR probes...
  25. ncbi A use of Ramachandran potentials in protein solution structure determinations
    Ivano Bertini
    Magnetic Resonance Center, University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Italy
    J Biomol NMR 26:355-66. 2003
    ..The quality of the structures, and of Ramachandran plot statistics in particular, was notably improved while preserving the agreement with the experimental constraints...
  26. ncbi X-ray structures of binary and ternary enzyme-product-inhibitor complexes of matrix metalloproteinases
    Ivano Bertini
    Magnetic Resonance Center and Department of Chemistry, University of Florence, Via Luigi Sacconi, 6, 50019 Sesto Fiorentino, Italy
    Angew Chem Int Ed Engl 42:2673-6. 2003
  27. ncbi Persistent contrast enhancement by sterically stabilized paramagnetic liposomes in murine melanoma
    Ivano Bertini
    Magnetic Resonance Center CERM, University of Florence, Italy
    Magn Reson Med 52:669-72. 2004
    ..High persistence of the CA was also observed in the liver and intestine, as expected in a hepatobiliar excretion pathway...
  28. ncbi The magnetic properties of myoglobin as studied by NMR spectroscopy
    Ivano Bertini
    CERM, University of Florence, Via Luigi Sacconi 6 50019 Sesto Fiorentino, Florence, Italy
    Chemistry 9:2316-22. 2003
    ....
  29. pmc Bioinorganic chemistry in the postgenomic era
    Ivano Bertini
    Magnetic Resonance Center, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Italy
    Proc Natl Acad Sci U S A 100:3601-4. 2003
    ..The "case study" of metal homeostasis in cells is discussed to provide a flavor of the current evolution of the field...
  30. ncbi A high-resolution NMR study of long-lived water molecules in both oxidation states of a minimal cytochrome c
    Ivano Bertini
    Magnetic Resonance Center CERM, University of Florence, Via L Sacconi 6, 50019 Sesto Fiorentino, Italy
    Biochemistry 42:3457-63. 2003
    ....
  31. ncbi NMR-validated structural model for oxidized Rhodopseudomonas palustris cytochrome c(556)
    Ivano Bertini
    CERM and Department of Chemistry, University of Florence, Via L Sacconi 6, 50019 Sesto Fiorentino, Italy
    J Biol Inorg Chem 9:224-30. 2004
    ..The directions and magnitude of the magnetic susceptibility anisotropy tensor were also calculated. The approach readily provides useful results, especially for paramagnetic metalloproteins of moderate to large dimensions...
  32. doi High-resolution solid-state NMR structure of a 17.6 kDa protein
    Ivano Bertini
    Magnetic Resonance Center, CERM, University of Florence, Via L Sacconi, 6 50019 Sesto Fiorentino, Italy
    J Am Chem Soc 132:1032-40. 2010
    ..3 A). The proposed strategy, which may be generalized for nonmetalloproteins with the use of paramagnetic tags, represents a significant step ahead in protein structure determination using solid-state NMR...
  33. ncbi Cross correlation rates between Curie spin and dipole-dipole relaxation in paramagnetic proteins: the case of cerium substituted calbindin D9k
    Ivano Bertini
    Magnetic Resonance Center, University of Florence, Italy
    J Biomol NMR 23:115-25. 2002
    ..This integrated structure calculation protocol has the advantage that different paramagnetic-based constraints are treated by the same algorithm in a way that the efficiency of each class of constraints can be analyzed and compared...
  34. pmc The anti-apoptotic Bcl-x(L) protein, a new piece in the puzzle of cytochrome c interactome
    Ivano Bertini
    Magnetic Resonance Center CERM, University of Florence, Sesto Fiorentino, Florence, Italy
    PLoS ONE 6:e18329. 2011
    ..The present model provides insights to the mechanism by which cytochrome c translocated to cytosol can be intercepted, so that the apoptosome is not assembled...
  35. ncbi Browsing gene banks for Fe2S2 ferredoxins and structural modeling of 88 plant-type sequences: an analysis of fold and function
    Ivano Bertini
    Centro di Risonanze Magnetiche, University of Florence, Sesto Fiorentino, Italy
    Proteins 46:110-27. 2002
    ....
  36. ncbi Heme methyl 1H chemical shifts as structural parameters in some low-spin ferriheme proteins
    I Bertini
    Center for Magnetic Resonance, Department of Chemistry, University of Florence, Italy
    J Biol Inorg Chem 4:515-9. 1999
    ..Therefore, by taking advantage of this study, information on the position of the axial ligands, that can be used as a constraint for structure determination, can be obtained from the shifts of the methyl protons...
  37. ncbi Model-free analysis of a thermophilic Fe(7)S(8) protein compared with a mesophilic Fe(4)S(4) protein
    I Bertini
    Department of Chemistry, University of Florence, Florence, Italy
    Proteins 41:75-85. 2000
    ..This research shows that subnanosecond rigidity is not related to thermostability and provides an estimate of the effect of increasing temperature on this time scale...
  38. ncbi Protein hydration and location of water molecules in oxidized horse heart cytochrome c by (1)H NMR
    I Bertini
    Department of Chemistry, Magnetic Resonance Center CERM, University of Florence, Via Luigi Sacconi 6, Florence, 50019 Sesto Fiorentino, Italy
    J Magn Reson 147:1-8. 2000
    ..The identification of hydrophilic regions and detection of three long-lived water molecules settles some ambiguities and provides a better representation of the water-protein interactions in oxidized cytochrome c...
  39. ncbi 15N chemical shift changes in cytochrome b5: redox-dependent vs. guanidinium chloride-induced changes
    I Bertini
    Magnetic Resonance Center, University of Florence, Sesto Fiorentino, Italy
    J Biol Inorg Chem 5:761-4. 2000
    ..It is concluded that changes in hydrogen bonding upon reduction must be modest and cannot account for the observed chemical shift effects. Alternative explanations should thus be looked for...
  40. ncbi Lanthanide induced residual dipolar couplings for the conformational investigation of peripheral 15NH2 moieties
    I Bertini
    Magnetic Resonance Center CERM, University of Florence, Italy
    J Biomol NMR 18:347-55. 2000
    ..The exploitation of auto-orientation of magnetic anisotropic metalloproteins represents a step ahead in the investigation of the conformational space of peripheral residues that are not fixed by the protein folding...
  41. doi Bioinformatics in bioinorganic chemistry
    Ivano Bertini
    Magnetic Resonance Center CERM University of Florence, Via L Sacconi 6, Sesto Fiorentino, Italy
    Metallomics 2:39-51. 2010
    ..We show how metalloproteomes can be extracted from genome sequences, how structural properties can be related to function, how databases can be implemented, and how hints on interactions can be obtained from bioinformatics...
  42. doi The annotation of full zinc proteomes
    Ivano Bertini
    Magnetic Resonance Center CERM, Department of Chemistry, University of Florence, Via L Sacconi 6, 50019, Sesto Fiorentino, Italy
    J Biol Inorg Chem 15:1071-8. 2010
    ..Among the findings, we have propose a zinc binding site for archaeal zinc-importing proteins. Furthermore, we propose two groups of transcriptional regulators that are involved in fatty acid metabolism...
  43. ncbi Solution structure of the Cu(I) and apo forms of the yeast metallochaperone, Atx1
    F Arnesano
    Magnetic Resonance Center CERM, University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Florence, Italy
    Biochemistry 40:1528-39. 2001
    ..L., Hou, M. Y., Wernimont, A. K., Pufahl, R. A., and O'Halloran, T. V. (1999) Structure 7, 605-617] provides insights into the copper transfer mechanism, and a pivotal role for Lys65 in the metal capture and release process is proposed...
  44. ncbi Copper trafficking: the solution structure of Bacillus subtilis CopZ
    L Banci
    Centro di Risonanze Magnetiche and Department of Chemistry, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Italy
    Biochemistry 40:15660-8. 2001
    ..The nature and the location of conserved residues around the metal-binding site are discussed with respect to their relevance for the metal-binding process. Proposals for the role of CopZ are also presented...
  45. ncbi Solution structure of reduced monomeric Q133M2 copper, zinc superoxide dismutase (SOD). Why is SOD a dimeric enzyme?
    L Banci
    Department of Chemistry, University of Florence, Italy
    Biochemistry 37:11780-91. 1998
    ..The hydrogen atom which binds the histidine nitrogen detached from copper(I) is structurally identified...
  46. ncbi Backbone dynamics of plastocyanin in both oxidation states. Solution structure of the reduced form and comparison with the oxidized state
    I Bertini
    Magnetic Resonance Center and Department of Chemistry, University of Florence, Via L Sacconi, 6 50019 Sesto Fiorentino, Italy
    J Biol Chem 276:47217-26. 2001
    ..The present characterization provides information on both the structural and dynamic behavior of blue copper proteins in solution that is useful to understand further the role(s) of protein dynamics in electron transfer processes...
  47. ncbi The three-dimensional solution structure of the reduced high-potential iron-sulfur protein from Chromatium vinosum through NMR
    L Banci
    Department of Chemistry, University of Florence, Italy
    Biochemistry 34:206-19. 1995
    ..These values compare well with those for diamagnetic proteins of the same size. The solution structure is discussed in the light of the available structural information from X-ray data...
  48. ncbi Three-dimensional structure of the reduced C77S mutant of the Chromatium vinosum high-potential iron-sulfur protein through nuclear magnetic resonance: comparison with the solution structure of the wild-type protein
    D Bentrop
    Department of Chemistry, University of Florence, Italy
    Biochemistry 35:5928-36. 1996
    ..Capillary electrophoresis experiments demonstrate coordination of Ser-77 as an anion. Serine versus cysteine coordination in iron-sulfur proteins is briefly discussed...
  49. ncbi (15)N backbone dynamics of ferricytochrome b(562): comparison with the reduced protein and the R98C variant
    M Assfalg
    Magnetic Resonance Center and Department of Chemistry, University of Florence, Sesto Fiorentino, Italy
    Biochemistry 40:12761-71. 2001
    ..It appears that the increased protein stability of the variant, established previously, is not correlated with an increase in rigidity...
  50. ncbi ePHOGSY experiments on a paramagnetic protein: location of the catalytic water molecule in the heme crevice of the oxidized form of horse heart cytochrome c
    I Bertini
    Department of Chemistry, University of Florence, Italy
    FEBS Lett 415:45-8. 1997
    ..The resulting picture is that there is a detectable movement of the water molecule upon oxidation...
  51. ncbi The solution structure of oxidized rat microsomal cytochrome b5
    F Arnesano
    Department of Chemistry, University of Florence, Italy
    Biochemistry 37:173-84. 1998
    ..This observation could suggest that, to optimize the electron transfer process, the local mobility should be properly tuned...
  52. ncbi The solution structure of oxidized Escherichia coli cytochrome b562
    F Arnesano
    Department of Chemistry, Centro di Risonanze Magnetiche, University of Florence, Italy
    Biochemistry 38:8657-70. 1999
    ..Quite intriguing is the comparison of the structure of cytochrome b562 with the available structures of cytochromes c' which display a similar folding motif and similar pKa values but very little sequence similarity...
  53. ncbi Solution structure of the yeast copper transporter domain Ccc2a in the apo and Cu(I)-loaded states
    L Banci
    Magnetic Resonance Center and Department of Chemistry, University of Florence, Via Luigi Sacconi 6, Sesto Fiorentino, Florence, 50019, Italy
    J Biol Chem 276:8415-26. 2001
    ..These results open new mechanistic aspects of copper transporter domains with physiological copper donor and acceptor proteins...
  54. ncbi Solution structure of reduced microsomal rat cytochrome b5
    L Banci
    Department of Chemistry, University of Florence, Italy
    Eur J Biochem 249:270-9. 1997
    ..The structural features in solution of the residues proposed to be involved in protein-protein recognition are found to be largely conserved with respect to the solid state...
  55. ncbi Three-dimensional solution structure of the oxidized high potential iron-sulfur protein from Chromatium vinosum through NMR. Comparative analysis with the solution structure of the reduced species
    I Bertini
    Department of Chemistry, University of Florence, Italy
    Biochemistry 34:9851-8. 1995
    ..This is the first time that independently determined solution structures of two redox states of a paramagnetic protein are available. Differences between them and the X-ray structure are discussed...
  56. ncbi Solution structure calculations through self-orientation in a magnetic field of a cerium(III) substituted calcium-binding protein
    I Bertini
    Department of Chemistry, University of Florence, Via Gino Capponi 9, Florence, 50121, Italy
    J Magn Reson 148:23-30. 2001
    ..This paper shows that Ce(III), like low-spin Fe(III) in hemoproteins, is sufficiently magnetically anisotropic to induce self-orientation to an extent which can be exploited for solution structure determination...
  57. ncbi Side chain mobility as monitored by CH-CH cross correlation: the example of cytochrome b5
    L Banci
    Magnetic Resonance Center (CERM, University of Florence, Sesto Fiorentino, Italy
    J Biomol NMR 20:1-10. 2001
    ..The general pattern is the same for the oxidized and reduced species, indicating that any oxidation-dependent property detected for backbone NH moieties does not affect the CH2 mobility...
  58. ncbi Characterization of a partially unfolded high potential iron protein
    I Bertini
    Department of Chemistry, University of Florence, via Gino Capponi, 7, 50121 Florence, Italy
    Biochemistry 36:9332-9. 1997
    ....
  59. ncbi Solution structure and backbone dynamics of the Cu(I) and apo forms of the second metal-binding domain of the Menkes protein ATP7A
    Lucia Banci
    Magnetic Resonance Center, University of Florence, Via L Sacconi 6, 50019 Sesto Fiorentino, Italy
    Biochemistry 43:3396-403. 2004
    ..A comparison with metallochaperones and soluble domains of P-type ATPases allows us to relate the primary structure to the occurrence of structural rearrangements upon copper binding...
  60. ncbi Solution structure of Sco1: a thioredoxin-like protein Involved in cytochrome c oxidase assembly
    Erica Balatri
    Magnetic Resonance Center CERM and Department of Chemistry, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Florence, Italy
    Structure 11:1431-43. 2003
    ..The availability of the structure has allowed us to model the homologs Sco1 and Sco2 from S. cerevisiae and to discuss the physiological role of the Sco family...
  61. ncbi The unfolding of oxidized c-type cytochromes: the instructive case of Bacillus pasteurii
    Ilaria Bartalesi
    Magnetic Resonance Center, University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Italy
    J Mol Biol 321:693-701. 2002
    ..It is concluded that the thermodynamic stability of the region around the metal cofactor is determined by the chemical nature of the residues around the axial methionine residue...
  62. ncbi Characterization of the binding interface between the copper chaperone Atx1 and the first cytosolic domain of Ccc2 ATPase
    F Arnesano
    Magnetic Resonance Center CERM and Department of Chemistry, University of Florence, Via Luigi Sacconi 6, Sesto Fiorentino, Florence 50019, Italy
    J Biol Chem 276:41365-76. 2001
    ..The analogous mobility of Ccc2a in both Cu(I) and apo forms is reduced with respect to Atx1. Such an adduct is relevant as a structural and kinetic model for copper transfer from Atx1 to Ccc2a in physiological conditions...
  63. ncbi Magnetic susceptibility tensor anisotropies for a lanthanide ion series in a fixed protein matrix
    I Bertini
    Department of Chemistry, University of Florence, Via Gino Capponi 9, 50121, Florence, Italy
    J Am Chem Soc 123:4181-8. 2001
    ..A knowledge of magnetic susceptibility anisotropy properties of lanthanides is essential in determining the self-orienting properties of lanthanide complexes in solution when immersed in magnetic fields...
  64. ncbi Solution structure of oxidized microsomal rabbit cytochrome b5. Factors determining the heterogeneous binding of the heme
    L Banci
    Department of Chemistry and Centro di Risonanze Magnetiche, University of Florence, Sesto Fiorentino, Italy
    Eur J Biochem 267:755-66. 2000
    ..Hydrophobic and steric interactions are the key factors in determining the relative stability of one isomer with respect to the other...
  65. ncbi The crystal structure of the monomeric human SOD mutant F50E/G51E/E133Q at atomic resolution. The enzyme mechanism revisited
    M Ferraroni
    Department of Chemistry, University of Florence, Via Gino Capponi 9, Florence, I 50121, Italy
    J Mol Biol 288:413-26. 1999
    ..These properties of the structure have allowed us to revisit the enzymatic mechanism...
  66. ncbi Solution structure of Apo Cu,Zn superoxide dismutase: role of metal ions in protein folding
    Lucia Banci
    Department of Chemistry and Centro Risonanze Magnetiche, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Italy
    Biochemistry 42:9543-53. 2003
    ....
  67. ncbi The evolutionarily conserved trimeric structure of CutA1 proteins suggests a role in signal transduction
    Fabio Arnesano
    Magnetic Resonance Center CERM and Department of Chemistry, University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Florence, Italy
    J Biol Chem 278:45999-6006. 2003
    ..This similarity suggests an intriguing role of CutA1 proteins in signal transduction through allosteric communications between subunits...
  68. ncbi Solution structure of apo CopZ from Bacillus subtilis: further analysis of the changes associated with the presence of copper
    Lucia Banci
    Department of Chemistry and Centro di Risonanze Magnetiche, University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Italy
    Biochemistry 42:13422-8. 2003
    ..It is also shown that copper(I) exchanges among apo CopZ molecules in slow exchange on the NMR time scale, whereas it is known that such exchange between partner molecules (i.e., metallochaperones and metal pumps) is fast...
  69. ncbi Protein stability and mutations in the axial methionine loop of a minimal cytochrome c
    Ilaria Bartalesi
    Magnetic Resonance Center, University of Florence, Via L Sacconi 6, 50019 Sesto Fiorentino, Italy
    J Biol Inorg Chem 9:600-8. 2004
    ..While the structure remains essentially the same, the stability decreases with mutations. The comparison with mitochondrial c-type cytochromes is instructive...
  70. ncbi Solution structure of Cox11, a novel type of beta-immunoglobulin-like fold involved in CuB site formation of cytochrome c oxidase
    Lucia Banci
    Magnetic Resonance Center CERM and Department of Chemistry, University of Florence, Via Luigi Sacconi 6, Sesto Fiorentino 50019, Florence, Italy
    J Biol Chem 279:34833-9. 2004
    ..The present results advance the knowledge on the poorly understood molecular aspects of cytochrome c oxidase assembly...
  71. ncbi A NMR study of the interaction of a three-domain construct of ATP7A with copper(I) and copper(I)-HAH1: the interplay of domains
    Lucia Banci
    Magnetic Resonance Center CERM, University of Florence, Via L Sacconi 6, 50019 Sesto Fiorentino, Italy
    J Biol Chem 280:38259-63. 2005
    ..This dependence could constitute the molecular mechanism to trigger copper(I) translocation and/or ATP7A relocalization from the trans-Golgi network to the plasmatic membrane...
  72. pmc 15N-1H Residual dipolar coupling analysis of native and alkaline-K79A Saccharomyces cerevisiae cytochrome c
    Michael Assfalg
    Magnetic Resonance Center CERM, University of Florence, 50019 Sesto Fiorentino, Florence, Italy
    Biophys J 84:3917-23. 2003
    ..Importantly, this is the first time that mobility has been found through comparison of RDCs with pseudocontact shifts...
  73. ncbi A new zinc-protein coordination site in intracellular metal trafficking: solution structure of the Apo and Zn(II) forms of ZntA(46-118)
    Lucia Banci
    Magnetic Resonance Center CERM and Department of Chemistry, University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Italy
    J Mol Biol 323:883-97. 2002
    ....
  74. ncbi Fully metallated S134N Cu,Zn-superoxide dismutase displays abnormal mobility and intermolecular contacts in solution
    Lucia Banci
    Magnetic Resonance Center, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Italy
    J Biol Chem 280:35815-21. 2005
    ..We have here also established that the two free cysteines (6 and 111) do not play a role in this behavior...
  75. ncbi Dimethyl propionate ester heme-containing cytochrome b5: structure and stability
    L Banci
    Magnetic Resonance Center, University of Florence, Italy
    J Biol Inorg Chem 6:490-503. 2001
    ..The available data on the reduction potential and the electron transfer rates are discussed on the basis of the present structural data...
  76. ncbi Solution structure of oxidized horse heart cytochrome c
    L Banci
    Department of Chemistry, University of Florence, Italy
    Biochemistry 36:9867-77. 1997
    ..cerevisiae and to previous results...
  77. ncbi 1H NMR studies of Chromatium vinosum cytochrome c'
    I Bertini
    Department of Chemistry, University of Florence, Firenze, Italy
    Arch Biochem Biophys 282:84-90. 1990
    ..Some evidence is provided for conformational flexibilities. Among the oxidized cytochromes c' the present one is capable of binding cyanide, giving rise to a low spin state. The reduced species is a typical high spin iron(II) system...
  78. ncbi Solution structure of the oxidized 2[4Fe-4S] ferredoxin from Clostridium pasteurianum
    I Bertini
    Department of Chemistry, University of Florence, Italy
    Eur J Biochem 232:192-205. 1995
    ..pasteurianum ferredoxin family is 78 pm (residues 3-53). Discrepancies in residues 26-28 may arise from the disorder observed in the X-ray structure in that region...
  79. ncbi An NMR study of the 7Fe-8S ferredoxin from Rhodopseudomonas palustris and reinterpretation of data on similar systems
    I Bertini
    Department of Chemistry, University of Florence, Italy
    Biochemistry 36:3570-9. 1997
    ..The NMR signal patterns of [3Fe-4S] clusters in both 3Fe-4S and 7Fe-8S ferredoxins have been discussed, and some correlations are proposed between signal patterns and the primary sequence...
  80. ncbi Ortholog search of proteins involved in copper delivery to cytochrome C oxidase and functional analysis of paralogs and gene neighbors by genomic context
    Fabio Arnesano
    Magnetic Resonance Center CERM and Department of Chemistry, University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Florence, Italy
    J Proteome Res 4:63-70. 2005
    ..By linking the assembly system to the copper uptake system, Sco allows COX to face alternative copper trafficking pathways...
  81. ncbi Paramagnetism-based refinement strategy for the solution structure of human alpha-parvalbumin
    Irfan Baig
    Magnetic Resonance Centre and Department of Chemistry, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Italy
    Biochemistry 43:5562-73. 2004
    ..0 A resolution. All differences are related to local changes in the amino acidic composition...
  82. ncbi Structural interplay between calcium(II) and copper(II) binding to S100A13 protein
    Fabio Arnesano
    Magnetic Resonance Center CERM and Department of Chemistry, University of Florence, Via L Sacconi 6, 50019 Sesto Fiorentino, Italy
    Angew Chem Int Ed Engl 44:6341-4. 2005
  83. ncbi Human SOD1 before harboring the catalytic metal: solution structure of copper-depleted, disulfide-reduced form
    Lucia Banci
    Magnetic Resonance Center, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Florence, Italy
    J Biol Chem 281:2333-7. 2006
    ..The structure allows us to further discuss the copper loading mechanism in SOD1...
  84. ncbi Solution structure of a monoheme ferrocytochrome c from Shewanella putrefaciens and structural analysis of sequence-similar proteins: functional implications
    Ilaria Bartalesi
    Centro di Risonanze Magnetiche, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Italy
    Biochemistry 41:5112-9. 2002
    ..The structures of the others have been modeled by using the available templates and internally validated. Structural similarities in terms of surface properties account for their biophysical features and provide hints about the function...
  85. ncbi Understanding copper trafficking in bacteria: interaction between the copper transport protein CopZ and the N-terminal domain of the copper ATPase CopA from Bacillus subtilis
    Lucia Banci
    Department of Chemistry, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino Florence, Italy
    Biochemistry 42:1939-49. 2003
    ..Comparing functional data of homologous proteins of other bacteria, it can be concluded that this class of proteins is involved in copper homeostasis but the specific roles are species dependent...
  86. ncbi A 15N NMR mobility study on the dicalcium P43M calbindin D9k and its mono-La3+-substituted form
    Ivano Bertini
    Magnetic Resonance Center CERM and Department of Chemistry, University of Florence, Sesto Fiorentino, Italy
    Biochemistry 41:5104-11. 2002
    ..They also demonstrate that substitution of a lanthanide ion for calcium, which is a common procedure, does not significantly alter the mobility of the native protein...
  87. ncbi Solution structure of the N-terminal domain of a potential copper-translocating P-type ATPase from Bacillus subtilis in the apo and Cu(I) loaded states
    Lucia Banci
    Magnetic Resonance Center CERM and Department of Chemistry, University of Florence, Via Luigi Sacconi 6, Florence, Sesto Fiorentino, 50019, Italy
    J Mol Biol 317:415-29. 2002
    ....
  88. ncbi Structure and dynamics of reduced Bacillus pasteurii cytochrome c: oxidation state dependent properties and implications for electron transfer processes
    Ilaria Bartalesi
    Centro di Risonanze Magnetiche, University of Florence, Sesto Fiorentino, Italy
    Biochemistry 42:739-45. 2003
    ..By comparing the redox-state dependence of the structural/dynamic properties of Fe-S proteins, cytochrome c, and blue copper proteins, hints are provided for a better comprehension of the electron transfer processes...
  89. ncbi Dynamic properties of the G93A mutant of copper-zinc superoxide dismutase as detected by NMR spectroscopy: implications for the pathology of familial amyotrophic lateral sclerosis
    Eric L Shipp
    Magnetic Resonance Center CERM, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Italy
    Biochemistry 42:1890-9. 2003
    ..Alternatively, a motor neuron-specific substrate may bind this region of the protein, leading to deleterious modifications and/or reactions...
  90. ncbi A hint to search for metalloproteins in gene banks
    Claudia Andreini
    Magnetic Resonance Center CERM, University of Florence, Italy
    Bioinformatics 20:1373-80. 2004
    ..Sometimes, metal binding can be observed in vitro but not be physiologically relevant. At present, there is a lack of specific tools to address the matter of the identification of metalloproteins in databases of gene sequences...
  91. pmc A prokaryotic superoxide dismutase paralog lacking two Cu ligands: from largely unstructured in solution to ordered in the crystal
    Lucia Banci
    Department of Chemistry, University of Florence, Via Luigi Sacconi 6, 50019 Sesto, Florence, Italy
    Proc Natl Acad Sci U S A 102:7541-6. 2005
    ..Couples of dimers held by hydrophobic interactions and H bonds are further organized in long chains. The order/disorder transition is discussed in terms of metal binding and physical state...
  92. ncbi A structural-dynamical characterization of human Cox17
    Lucia Banci
    Magnetic Resonance Center Centro Risonanze Magnetiche CERM and Department of Chemistry, University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Florence, Italy
    J Biol Chem 283:7912-20. 2008
    ..The copper(I) form of Cox17(2S-S) has features specific for copper chaperones...
  93. ncbi Metalation of the amyotrophic lateral sclerosis mutant glycine 37 to arginine superoxide dismutase (SOD1) apoprotein restores its structural and dynamical properties in solution to those of metalated wild-type SOD1
    Lucia Banci
    Magnetic Resonance Center, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Italy
    Biochemistry 46:9953-62. 2007
    ..These results suggest further that it is the metal-free apo forms of the mutant SOD1 protein that are the agents of its toxicity...
  94. ncbi Occurrence of copper proteins through the three domains of life: a bioinformatic approach
    Claudia Andreini
    Magnetic Resonance Center CERM and Department of Chemistry, University of Florence, Via L Sacconi 6, 50019 Sesto Fiorentino, Italy
    J Proteome Res 7:209-16. 2008
    ..A network involving proteins having roles in both copper transport and respiration was identified, parts or all of which are detected in the majority of the organisms examined...
  95. ncbi Superoxide dismutase folding/unfolding pathway: role of the metal ions in modulating structural and dynamical features
    Michael Assfalg
    CERM, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Italy
    J Mol Biol 330:145-58. 2003
    ..4 M, thus demonstrating the importance of metal ions with respect to the unfolding process and protein structure stability. Hints to understand the whole folding process are obtained and discussed...
  96. ncbi Structural basis for the function of the N-terminal domain of the ATPase CopA from Bacillus subtilis
    Lucia Banci
    Magnetic Resonance Center CERM and Department of Chemistry, University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Florence, Italy
    J Biol Chem 278:50506-13. 2003
    ....
  97. ncbi A quick solution structure determination of the fully oxidized double mutant K9-10A cytochrome c7 from Desulfuromonas acetoxidans and mechanistic implications
    Michael Assfalg
    Magnetic Resonance Center and Department of Chemistry, University of Florence, Sesto Fiorentino, Italy
    J Biomol NMR 22:107-22. 2002
    ..This finding is in agreement with the identification of the protein area around heme IV as the interacting site...
  98. ncbi NMR structural analysis of cadmium sensing by winged helix repressor CmtR
    Lucia Banci
    Department of Chemistry and Centro Risonanze Magnetiche, University of Florence, Via Luigi Sacconi 6, Florence 50019, Italy
    J Biol Chem 282:30181-8. 2007
    ..Cadmium binding across the dimer interface impairs DNA association by excluding the apo-conformers suited to bind DNA...
  99. ncbi Zinc through the three domains of life
    Claudia Andreini
    Magnetic Resonance Center CERM, University of Florence, Via L Sacconi 6, 50019 Sesto Fiorentino, Italy
    J Proteome Res 5:3173-8. 2006
    ..8%) is significantly higher than that observed in Bacteria and Archaea (from 5% to 6%). Most of this enrichment is due to the larger portfolio of regulatory proteins in Eukaryota...
  100. ncbi Metallochaperones and metal-transporting ATPases: a comparative analysis of sequences and structures
    Fabio Arnesano
    Magnetic Resonance Center CERM and Department of Chemistry, University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Florence, Italy
    Genome Res 12:255-71. 2002
    ..This analysis highlights the wealth and the complexity of the field...
  101. doi Solution structures of the actuator domain of ATP7A and ATP7B, the Menkes and Wilson disease proteins
    Lucia Banci
    Magnetic Resonance Center CERM University of Florence, Via L Sacconi 6, 50019 Sesto Fiorentino, Italy
    Biochemistry 48:7849-55. 2009
    ....