Mirko Baruscotti

Summary

Affiliation: University of Milan
Country: Italy

Publications

  1. ncbi request reprint Distribution of the pacemaker HCN4 channel mRNA and protein in the rabbit sinoatrial node
    Chiara Brioschi
    University of Milano, Department of Biomolecular Sciences and Biotechnology, Laboratory of Molecular Physiology and Neurobiology, Via Celoria 26, 20133 Milano, Italy
    J Mol Cell Cardiol 47:221-7. 2009
  2. pmc Funny Current and Cardiac Rhythm: Insights from HCN Knockout and Transgenic Mouse Models
    Annalisa Bucchi
    Department of Biosciences, University of Milano Milano, Italy
    Front Physiol 3:240. 2012
  3. ncbi request reprint HCN-related channelopathies
    Mirko Baruscotti
    Department of Biomolecular Sciences and Biotechnology, The PaceLab, University of Milano and Centro Interuniversitario di Medicina Molecolare e Biofisica Applicata CIMMBA, Milano, Italy
    Pflugers Arch 460:405-15. 2010
  4. doi request reprint The cardiac pacemaker current
    Mirko Baruscotti
    Department of Biomolecular Sciences and Biotechnology, Laboratory of Molecular Physiology and Neurobiology, Universita degli Studi di Milano, Centro Interuniversitario di Medicina Molecolare e Biofisica Applicata CIMMBA, Via Celoria 26, 20133 Milano, Italy
    J Mol Cell Cardiol 48:55-64. 2010
  5. ncbi request reprint Single-channel properties of the sinoatrial node Na+ current in the newborn rabbit
    M Baruscotti
    Universita di Milano, Dipartimento di Fisiologia e Biochimica Generali, Elettrofisiologia, Via Celoria 26, 20133 Milano, Italy
    Pflugers Arch 442:192-6. 2001
  6. doi request reprint Embryonic stem cell-derived CD166+ precursors develop into fully functional sinoatrial-like cells
    Angela Scavone
    Department of Biosciences, Universita degli Studi di Milano, Milano, Italy
    Circ Res 113:389-98. 2013
  7. doi request reprint Molecular composition and functional properties of f-channels in murine embryonic stem cell-derived pacemaker cells
    Andrea Barbuti
    Department of Biomolecular Sciences and Biotechnology, Laboratory of Molecular Physiology and Neurobiology, University of Milano, Via Celoria 26, 20133 Milano, Italy
    J Mol Cell Cardiol 46:343-51. 2009
  8. doi request reprint Recessive loss-of-function mutation in the pacemaker HCN2 channel causing increased neuronal excitability in a patient with idiopathic generalized epilepsy
    Jacopo C DiFrancesco
    Dipartimento di Neuroscienze e Tecnologie Biomediche, Ospedale S Gerardo, Universita di Milano Bicocca, 20052 Monza, Italy
    J Neurosci 31:17327-37. 2011
  9. pmc Deep bradycardia and heart block caused by inducible cardiac-specific knockout of the pacemaker channel gene Hcn4
    Mirko Baruscotti
    Department of Biomolecular Sciences and Biotechnology, PaceLab and Centro Interuniversitario di Medicina Molecolare e Biofisica Applicata, Milano, Universita degli Studi di Milano, 20133 Milan, Italy
    Proc Natl Acad Sci U S A 108:1705-10. 2011
  10. ncbi request reprint Modulation of rate by autonomic agonists in SAN cells involves changes in diastolic depolarization and the pacemaker current
    Annalisa Bucchi
    University of Milano, Department of Biomolecular Sciences and Biotechnology, Laboratory of Molecular Physiology and Neurobiology, Via Celoria 26, 20133 Milano, Italy
    J Mol Cell Cardiol 43:39-48. 2007

Collaborators

Detail Information

Publications28

  1. ncbi request reprint Distribution of the pacemaker HCN4 channel mRNA and protein in the rabbit sinoatrial node
    Chiara Brioschi
    University of Milano, Department of Biomolecular Sciences and Biotechnology, Laboratory of Molecular Physiology and Neurobiology, Via Celoria 26, 20133 Milano, Italy
    J Mol Cell Cardiol 47:221-7. 2009
    ..Until recently, the SAN was identified as the region where Cx43 and atrial natriuretic peptide are not expressed; we propose here that expression of HCN4 is an appropriate tool to map and identify the cardiac SAN pacemaker region...
  2. pmc Funny Current and Cardiac Rhythm: Insights from HCN Knockout and Transgenic Mouse Models
    Annalisa Bucchi
    Department of Biosciences, University of Milano Milano, Italy
    Front Physiol 3:240. 2012
    ..These combined studies were not possible prior to the development of KO models. Finally, these models represent critical tools to improve our understanding of the molecular basis of some inheritable arrhythmic human pathologies...
  3. ncbi request reprint HCN-related channelopathies
    Mirko Baruscotti
    Department of Biomolecular Sciences and Biotechnology, The PaceLab, University of Milano and Centro Interuniversitario di Medicina Molecolare e Biofisica Applicata CIMMBA, Milano, Italy
    Pflugers Arch 460:405-15. 2010
    ..Here we briefly review the current knowledge of HCN-related channelopathies in the heart and the brain...
  4. doi request reprint The cardiac pacemaker current
    Mirko Baruscotti
    Department of Biomolecular Sciences and Biotechnology, Laboratory of Molecular Physiology and Neurobiology, Universita degli Studi di Milano, Centro Interuniversitario di Medicina Molecolare e Biofisica Applicata CIMMBA, Via Celoria 26, 20133 Milano, Italy
    J Mol Cell Cardiol 48:55-64. 2010
    ..In this review some of the most recent findings on f/HCN channels contribution to pacemaking are described...
  5. ncbi request reprint Single-channel properties of the sinoatrial node Na+ current in the newborn rabbit
    M Baruscotti
    Universita di Milano, Dipartimento di Fisiologia e Biochimica Generali, Elettrofisiologia, Via Celoria 26, 20133 Milano, Italy
    Pflugers Arch 442:192-6. 2001
    ..We suggest that this is the mechanism by which a small tetrodotoxin (TTX)-sensitive current contributes to the total inward current flowing during slow diastolic depolarization in neonatal (but not adult) pacemaker myocytes...
  6. doi request reprint Embryonic stem cell-derived CD166+ precursors develop into fully functional sinoatrial-like cells
    Angela Scavone
    Department of Biosciences, Universita degli Studi di Milano, Milano, Italy
    Circ Res 113:389-98. 2013
    ..So far, such selection has been hampered by the lack of proper markers. CD166 is specifically but transiently expressed in the mouse heart tube and sinus venosus, the prospective SAN...
  7. doi request reprint Molecular composition and functional properties of f-channels in murine embryonic stem cell-derived pacemaker cells
    Andrea Barbuti
    Department of Biomolecular Sciences and Biotechnology, Laboratory of Molecular Physiology and Neurobiology, University of Milano, Via Celoria 26, 20133 Milano, Italy
    J Mol Cell Cardiol 46:343-51. 2009
    ..Our data show that mESC-derived pacemaker cells functionally express proteins which underlie generation and modulation of heart rhythm, and can therefore represent a potential cell substrate for the generation of biological pacemakers...
  8. doi request reprint Recessive loss-of-function mutation in the pacemaker HCN2 channel causing increased neuronal excitability in a patient with idiopathic generalized epilepsy
    Jacopo C DiFrancesco
    Dipartimento di Neuroscienze e Tecnologie Biomediche, Ospedale S Gerardo, Universita di Milano Bicocca, 20052 Monza, Italy
    J Neurosci 31:17327-37. 2011
    ..This is the first evidence in humans for a single-point, homozygous loss-of-function mutation in HCN2 potentially associated with generalized epilepsy with recessive inheritance...
  9. pmc Deep bradycardia and heart block caused by inducible cardiac-specific knockout of the pacemaker channel gene Hcn4
    Mirko Baruscotti
    Department of Biomolecular Sciences and Biotechnology, PaceLab and Centro Interuniversitario di Medicina Molecolare e Biofisica Applicata, Milano, Universita degli Studi di Milano, 20133 Milan, Italy
    Proc Natl Acad Sci U S A 108:1705-10. 2011
    ..This work contributes to identifying the molecular mechanism responsible for cardiac pacemaking...
  10. ncbi request reprint Modulation of rate by autonomic agonists in SAN cells involves changes in diastolic depolarization and the pacemaker current
    Annalisa Bucchi
    University of Milano, Department of Biomolecular Sciences and Biotechnology, Laboratory of Molecular Physiology and Neurobiology, Via Celoria 26, 20133 Milano, Italy
    J Mol Cell Cardiol 43:39-48. 2007
    ..3% and Iso, +47.6%) and not of TOP. We conclude that rate-related changes in the EDD induced by autonomic agonists are mediated by I(f) and not by processes involving SR Ca2+ transients...
  11. ncbi request reprint Physiology and pharmacology of the cardiac pacemaker ("funny") current
    Mirko Baruscotti
    Laboratory of Molecular Physiology and Neurobiology, Department of Biomolecular Sciences and Biotechnology, University of Milano, Via Celoria 26, 20133 Milan, Italy
    Pharmacol Ther 107:59-79. 2005
    ....
  12. pmc Identification of the molecular site of ivabradine binding to HCN4 channels
    Annalisa Bucchi
    The PaceLab, Department of Life Sciences, Universita degli Studi di Milano, Milano, Italy
    PLoS ONE 8:e53132. 2013
    ....
  13. doi request reprint Mesoangioblasts from ventricular vessels can differentiate in vitro into cardiac myocytes with sinoatrial-like properties
    Andrea Barbuti
    Department of Biomolecular Sciences and Biotechnology, The PaceLab, University of Milano, Via Celoria 26, 20133 Milan, Italy
    J Mol Cell Cardiol 48:415-23. 2010
    ..These data represent the first evidence for in vitro generation of pacemaker-like myocytes from proliferating non-embryonic stem/progenitor cells...
  14. ncbi request reprint Electrophysiology and pacemaker function of the developing sinoatrial node
    Mirko Baruscotti
    Laboratory of Molecular Physiology and Neurobiology, Department of Biomolecular Sciences and Biotechnology, University of Milano, Milan, Italy
    Am J Physiol Heart Circ Physiol 293:H2613-23. 2007
    ....
  15. ncbi request reprint The pacemaker current: from basics to the clinics
    Andrea Barbuti
    Laboratory of Molecular Physiology and Neurobiology, Department of Biomolecular Sciences and Biotechnology, University of Milan, Milano, Italy
    J Cardiovasc Electrophysiol 18:342-7. 2007
    ..In this short review, we describe some of the most recent clinically relevant applications of the concept of I(f)-based pacemaking...
  16. ncbi request reprint Heart rate reduction via selective 'funny' channel blockers
    Annalisa Bucchi
    Laboratory of Molecular Physiology and Neurobiology, Department of Biomolecular Sciences and Biotechnology, University of Milan, Via Celoria 26, Milano, Italy
    Curr Opin Pharmacol 7:208-13. 2007
    ..Because of their many functions in heart and other tissues, pharmacological utilization of "funny" channel properties is an exciting new frontier open to further developments...
  17. pmc Heteromeric HCN1-HCN4 channels: a comparison with native pacemaker channels from the rabbit sinoatrial node
    Claudia Altomare
    Department of General Physiology and Biochemistry, Laboratory of Molecular Physiology and Neurobiology, Milano, Italy
    J Physiol 549:347-59. 2003
    ..HCN1 and HCN4 may contribute to native f-channels, but a 'context'-dependent mechanism is also likely to modulate the channel properties in native tissues...
  18. ncbi request reprint Familial sinus bradycardia associated with a mutation in the cardiac pacemaker channel
    Raffaella Milanesi
    Department of Biomolecular Sciences and Biotechnology, Laboratory of Molecular Physiology and Neurobiology, University of Milan, Milan, Italy
    N Engl J Med 354:151-7. 2006
    ..These changes, which mimic those of mild vagal stimulation, slow the heart rate by decreasing the inward diastolic current. Thus, diminished function of pacemaker channels is linked to familial bradycardia...
  19. doi request reprint A caveolin-binding domain in the HCN4 channels mediates functional interaction with caveolin proteins
    Andrea Barbuti
    Department of Biomolecular Sciences and Biotechnology, The PaceLab, Universita degli Studi di Milano, Via Celoria 26, 20133 Milano, Italy
    J Mol Cell Cardiol 53:187-95. 2012
    ..The elucidation of the molecular details of HCN4-cav-1 interaction can provide novel information to understand the basis of cardiac phenotypes associated with some forms of caveolinopathies...
  20. ncbi request reprint I(f)-dependent modulation of pacemaker rate mediated by cAMP in the presence of ryanodine in rabbit sino-atrial node cells
    Annalisa Bucchi
    Department of General Physiology and Biochemistry, Laboratory of Molecular Physiology and Neurobiology, University of Milan, Via Celoria 26, Milano 20133, Italy
    J Mol Cell Cardiol 35:905-13. 2003
    ..The molecular basis for Ca(2+)-dependent interference in beta-adrenergic signaling remains to be determined...
  21. doi request reprint Human cord blood CD34+ progenitor cells acquire functional cardiac properties through a cell fusion process
    Daniele Avitabile
    Department of Biomolecular Sciences and Biotechnology, University of Milan, Milan, Italy
    Am J Physiol Heart Circ Physiol 300:H1875-84. 2011
    ....
  22. doi request reprint Multinucleated giant cells with an osteoclast phenotype derived from caprine peripheral blood mononuclear cells
    Giuliano Pisoni
    Universita degli Studi di Milano, Dipartimento di Patologia Animale, Igiene e Sanita Pubblica Veterinaria, Via Celoria 10, 20133 Milano, Italy
    Vet J 189:361-3. 2011
    ....
  23. ncbi request reprint Pacemaker channels
    Mirko Baruscotti
    Department of Biomolecular Sciences and Biotechnology, Laboratory of Molecular Physiology and Neurobiology, University of Milan, Italy
    Ann N Y Acad Sci 1015:111-21. 2004
    ..Experiments aimed at determining the role of I(f) relative to other proposed pacemaker mechanisms such as SR Ca(2+) transients confirm that the I(f)-mediated rate control is a key process in pacemaker generation and autonomic control...
  24. pmc Current-dependent block of rabbit sino-atrial node I(f) channels by ivabradine
    Annalisa Bucchi
    Department of General Physiology and Biochemistry, Laboratory of Molecular Physiology and Neurobiology, and INFM Unità Milano Università, 20133 Milano, Italy
    J Gen Physiol 120:1-13. 2002
    ..The use-dependence resulting from specific features of I(f) block by ivabradine amplifies its rate-reducing ability at high spontaneous rates and may be useful to clinical applications...
  25. pmc Direct inhibition of the pacemaker (If) current in rabbit sinoatrial node cells by genistein
    Claudia Altomare
    Department of Biomolecular Sciences and Biotechnology, Laboratory of Molecular Physiology and Neurobiology, University of Milan, Via Celoria 26, 20133 Milano, Italy
    Br J Pharmacol 147:36-44. 2006
    ....
  26. pmc Properties of ivabradine-induced block of HCN1 and HCN4 pacemaker channels
    A Bucchi
    Laboratory of Molecular Physiology and Neurobiology, Department of Biomolecular Sciences and Biotechnology, University of Milano, Italy
    J Physiol 572:335-46. 2006
    ..The mode of action of ivabradine on the two channels is discussed by implementing a simplified version of a previously developed model of f-channel kinetics...
  27. ncbi request reprint C terminus-mediated control of voltage and cAMP gating of hyperpolarization-activated cyclic nucleotide-gated channels
    C Viscomi
    Dipartimento di Fisiologia e Biochimica Generali, Laboratorio Fisiologia Molecolare e Neurobiologia, and INFM, , Via Celoria 26, 20133 Milano, Italy
    J Biol Chem 276:29930-4. 2001
    ..These results indicate that the C terminus of rbHCN4 contributes to the regulation of voltage- and cAMP-dependent channel gating, possibly through interaction with other intracellular regions not belonging to the N terminus...
  28. pmc Integrated allosteric model of voltage gating of HCN channels
    C Altomare
    Dipartimento di Fisiologia e Biochimica Generali, Via Celoria 26, , Via Celoria 16, 20133 Milano, Italy
    J Gen Physiol 117:519-32. 2001
    ..HCN voltage gating thus involves two processes: voltage sensor gating and allosteric opening/closing...