Giancarlo Aldini

Summary

Affiliation: University of Milan
Country: Italy

Publications

  1. doi request reprint Novel molecular approaches for improving enzymatic and nonenzymatic detoxification of 4-hydroxynonenal: toward the discovery of a novel class of bioactive compounds
    Giancarlo Aldini
    Department of Pharmaceutical Sciences, Universita degli Studi di Milano, 20133 Milan, Italy Electronic address
    Free Radic Biol Med 69:145-56. 2014
  2. ncbi request reprint Advanced analytical strategies for recombinant therapeutic proteins
    Giancarlo Aldini
    Dipartimento di Scienze Farmaceutiche Pietro Pratesi, University of Milan Via Mangiagalli 25, 20133 Milan, Italy
    Curr Pharm Biotechnol 12:1507. 2011
  3. doi request reprint Albumin is the main nucleophilic target of human plasma: a protective role against pro-atherogenic electrophilic reactive carbonyl species?
    Giancarlo Aldini
    Istituto di Chimica Farmaceutica e Tossicologica Pietro Pratesi, Faculty of Pharmacy, University of Milan, I 20131, Milan, Italy
    Chem Res Toxicol 21:824-35. 2008
  4. ncbi request reprint Profiling histidine-containing dipeptides in rat tissues by liquid chromatography/electrospray ionization tandem mass spectrometry
    Giancarlo Aldini
    Istituto Chimico Farmaceutico Tossicologico, Faculty of Pharmacy, University of Milan, Viale Abruzzi 42, 20131 Milan, Italy
    J Mass Spectrom 39:1417-28. 2004
  5. ncbi request reprint Covalent modification of actin by 4-hydroxy-trans-2-nonenal (HNE): LC-ESI-MS/MS evidence for Cys374 Michael adduction
    Giancarlo Aldini
    Istituto Chimico Farmaceutico Tossicologico, University of Milan, Viale Abruzzi 42, I 20131 Milan, Italy
    J Mass Spectrom 40:946-54. 2005
  6. doi request reprint An integrated high resolution mass spectrometric and informatics approach for the rapid identification of phenolics in plant extract
    Giancarlo Aldini
    Department of Pharmaceutical Sciences Pietro Pratesi, Universita degli Studi di Milano, Milan, Italy
    J Chromatogr A 1218:2856-64. 2011
  7. ncbi request reprint Carnosine and related dipeptides as quenchers of reactive carbonyl species: from structural studies to therapeutic perspectives
    Giancarlo Aldini
    Istituto Chimico Farmaceutico Tossicologico, Faculty of Pharmacy, University of Milan, Viale Abruzzi 42, 20131 Milan, Italy
    Biofactors 24:77-87. 2005
  8. doi request reprint The carbonyl scavenger carnosine ameliorates dyslipidaemia and renal function in Zucker obese rats
    Giancarlo Aldini
    Department of Pharmaceutical Sciences Pietro Pratesi, Universita degli Studi di Milano, Milan, Italy
    J Cell Mol Med 15:1339-54. 2011
  9. ncbi request reprint Effects of UVB radiation on 4-hydroxy-2-trans-nonenal metabolism and toxicity in human keratinocytes
    Giancarlo Aldini
    Istituto di Chimica Farmaceutica e Tossicologica Pietro Pratesi, Faculty of Pharmacy, University of Milan, Viale Abruzzi 42, I 20131 Milan, Italy
    Chem Res Toxicol 20:416-23. 2007
  10. ncbi request reprint A tandem MS precursor-ion scan approach to identify variable covalent modification of albumin Cys34: a new tool for studying vascular carbonylation
    Giancarlo Aldini
    Istituto di Chimica Farmaceutica e Tossicologica Pietro Pratesi, Faculty of Pharmacy, University of Milan, Via Mangiagalli 25, I 20133 Milan, Italy
    J Mass Spectrom 43:1470-81. 2008

Collaborators

Detail Information

Publications67

  1. doi request reprint Novel molecular approaches for improving enzymatic and nonenzymatic detoxification of 4-hydroxynonenal: toward the discovery of a novel class of bioactive compounds
    Giancarlo Aldini
    Department of Pharmaceutical Sciences, Universita degli Studi di Milano, 20133 Milan, Italy Electronic address
    Free Radic Biol Med 69:145-56. 2014
    ..In this review the various strategies are reported and discussed together with their limits and potentials. ..
  2. ncbi request reprint Advanced analytical strategies for recombinant therapeutic proteins
    Giancarlo Aldini
    Dipartimento di Scienze Farmaceutiche Pietro Pratesi, University of Milan Via Mangiagalli 25, 20133 Milan, Italy
    Curr Pharm Biotechnol 12:1507. 2011
    ....
  3. doi request reprint Albumin is the main nucleophilic target of human plasma: a protective role against pro-atherogenic electrophilic reactive carbonyl species?
    Giancarlo Aldini
    Istituto di Chimica Farmaceutica e Tossicologica Pietro Pratesi, Faculty of Pharmacy, University of Milan, I 20131, Milan, Italy
    Chem Res Toxicol 21:824-35. 2008
    ..81 +/- 0.17 M (-1) s (-1)), as explained by molecular modeling studies. In conclusion, we suggest that albumin, through nucleophilic residues, and in particular Cys34, can act as an endogenous detoxifying agent of circulating RCS...
  4. ncbi request reprint Profiling histidine-containing dipeptides in rat tissues by liquid chromatography/electrospray ionization tandem mass spectrometry
    Giancarlo Aldini
    Istituto Chimico Farmaceutico Tossicologico, Faculty of Pharmacy, University of Milan, Viale Abruzzi 42, 20131 Milan, Italy
    J Mass Spectrom 39:1417-28. 2004
    ..HCAR was found only in the brain and cerebellum. No histidine-containing dipeptides were detectable in plasma, liver, kidney and lung...
  5. ncbi request reprint Covalent modification of actin by 4-hydroxy-trans-2-nonenal (HNE): LC-ESI-MS/MS evidence for Cys374 Michael adduction
    Giancarlo Aldini
    Istituto Chimico Farmaceutico Tossicologico, University of Milan, Viale Abruzzi 42, I 20131 Milan, Italy
    J Mass Spectrom 40:946-54. 2005
    ..Computational studies showed that the reactivity of Cys374 residue is due to a significant accessible surface and substantial thiol acidity due to the particular microenvironment surrounding Cys374...
  6. doi request reprint An integrated high resolution mass spectrometric and informatics approach for the rapid identification of phenolics in plant extract
    Giancarlo Aldini
    Department of Pharmaceutical Sciences Pietro Pratesi, Universita degli Studi di Milano, Milan, Italy
    J Chromatogr A 1218:2856-64. 2011
    ..The method was then successfully applied for the rapid identification of phenolics contained in an EtOH extract of Angelica keiskei...
  7. ncbi request reprint Carnosine and related dipeptides as quenchers of reactive carbonyl species: from structural studies to therapeutic perspectives
    Giancarlo Aldini
    Istituto Chimico Farmaceutico Tossicologico, Faculty of Pharmacy, University of Milan, Viale Abruzzi 42, 20131 Milan, Italy
    Biofactors 24:77-87. 2005
    ....
  8. doi request reprint The carbonyl scavenger carnosine ameliorates dyslipidaemia and renal function in Zucker obese rats
    Giancarlo Aldini
    Department of Pharmaceutical Sciences Pietro Pratesi, Universita degli Studi di Milano, Milan, Italy
    J Cell Mol Med 15:1339-54. 2011
    ....
  9. ncbi request reprint Effects of UVB radiation on 4-hydroxy-2-trans-nonenal metabolism and toxicity in human keratinocytes
    Giancarlo Aldini
    Istituto di Chimica Farmaceutica e Tossicologica Pietro Pratesi, Faculty of Pharmacy, University of Milan, Viale Abruzzi 42, I 20131 Milan, Italy
    Chem Res Toxicol 20:416-23. 2007
    ....
  10. ncbi request reprint A tandem MS precursor-ion scan approach to identify variable covalent modification of albumin Cys34: a new tool for studying vascular carbonylation
    Giancarlo Aldini
    Istituto di Chimica Farmaceutica e Tossicologica Pietro Pratesi, Faculty of Pharmacy, University of Milan, Via Mangiagalli 25, I 20133 Milan, Italy
    J Mass Spectrom 43:1470-81. 2008
    ....
  11. ncbi request reprint Mass spectrometric characterization of covalent modification of human serum albumin by 4-hydroxy-trans-2-nonenal
    Giancarlo Aldini
    Istituto di Chimica Farmaceutica e Tossicologica Pietro Pratesi, Faculty of Pharmacy, University of Milan, Viale Abruzzi 42, I 20131 Milan, Italy
    J Mass Spectrom 41:1149-61. 2006
    ..These albumin modifications are suitable tags of HNE-adducted albumin and could be useful biomarkers of oxidative and carbonylation damage in humans...
  12. ncbi request reprint Intervention strategies to inhibit protein carbonylation by lipoxidation-derived reactive carbonyls
    Giancarlo Aldini
    Institute of Pharmaceutical and Toxicological Chemistry, Faculty of Pharmacy, University of Milan, Viale Abruzzi 42, I 20131, Milan, Italy
    Med Res Rev 27:817-68. 2007
    ..Inhibition of ALE formation, based on carbonyl-sequestering agents, seems to be the most promising pharmacological tool and is reviewed in detail...
  13. ncbi request reprint Alpha,beta-unsaturated aldehydes adducts to actin and albumin as potential biomarkers of carbonylation damage
    Giancarlo Aldini
    Istituto di Chimica Farmaceutica e Tossicologica Pietro Pratesi, Faculty of Pharmacy, University of Milan, Milan, Italy
    Redox Rep 12:20-5. 2007
    ....
  14. ncbi request reprint Identification of actin as a 15-deoxy-Delta12,14-prostaglandin J2 target in neuroblastoma cells: mass spectrometric, computational, and functional approaches to investigate the effect on cytoskeletal derangement
    Giancarlo Aldini
    Istituto di Chimica Farmaceutica e Tossicologica Pietro Pratesi, Faculty of Pharmacy, University of Milan, I 20131 Milan, Italy
    Biochemistry 46:2707-18. 2007
    ....
  15. ncbi request reprint Electron spin resonance and chemiluminescence analyses to elucidate the vasodilating mechanism of sodium nitroprusside
    Giancarlo Aldini
    Istituto di Chimica Farmaceutica e Tossicologica Pietro Pratesi, Faculty of Pharmacy, University of Milan, Viale Abruzzi 42, 20131 Milan, Italy
    Mol Pharmacol 70:1672-80. 2006
    ..In conclusion, SNP is rapidly metabolized to HbFe(II)NO, but the pharmacological response is mediated by a direct mechanism of NO release of the parent compound at the cellular target...
  16. ncbi request reprint Detoxification of cytotoxic alpha,beta-unsaturated aldehydes by carnosine: characterization of conjugated adducts by electrospray ionization tandem mass spectrometry and detection by liquid chromatography/mass spectrometry in rat skeletal muscle
    Giancarlo Aldini
    Istituto Chimico Farmaceutico Tossicologico, University of Milan, Viale Abruzzi 42, 20131 Milan, Italy
    J Mass Spectrom 37:1219-28. 2002
    ..The results indicate that beside GSH, histidine-containing dipeptides could be involved in the detoxification pathway of reactive aldehydes from lipid peroxidation generated in skeletal muscle during physical endurance...
  17. ncbi request reprint Carnosine is a quencher of 4-hydroxy-nonenal: through what mechanism of reaction?
    Giancarlo Aldini
    Istituto Chimico Farmaceutico Tossicologico, University of Milan, Viale Abruzzi 42, 20131 Milan, Italy
    Biochem Biophys Res Commun 298:699-706. 2002
    ..The biological role of carnosine as a quencher of alpha,beta-unsaturated aldehydes was verified by detecting carnosine-HNE reaction adducts in oxidized rat skeletal muscle homogenate...
  18. ncbi request reprint Metabolic profile of NO-flurbiprofen (HCT1026) in rat brain and plasma: a LC-MS study
    Giancarlo Aldini
    Istituto Chimico Farmaceutico Tossicologico, University of Milan, Viale Abruzzi 42, 20131 Milan, Italy
    Life Sci 71:1487-500. 2002
    ..These data indicate that the NO molecule is not released from the parent drug within the brain...
  19. ncbi request reprint Detoxification of 4-hydroxynonenal (HNE) in keratinocytes: characterization of conjugated metabolites by liquid chromatography/electrospray ionization tandem mass spectrometry
    Giancarlo Aldini
    Istituto Chimico Farmaceutico Tossicologico, University of Milan, Viale Abruzzi 42, 20131 Milan, Italy
    J Mass Spectrom 38:1160-8. 2003
    ..This is the first demonstration that skin epidermal cells are able to detoxify the cytotoxic products of lipid peroxidation...
  20. ncbi request reprint Procyanidins from grape seeds protect endothelial cells from peroxynitrite damage and enhance endothelium-dependent relaxation in human artery: new evidences for cardio-protection
    Giancarlo Aldini
    Istituto Chimico Farmaceutico Tossicologico, University of Milan, Viale Abruzzi 42 20131 Milan, Italy
    Life Sci 73:2883-98. 2003
    ....
  21. ncbi request reprint Nitrosylhemoglobin formation after infusion of NO solutions: ESR studies in pigs
    Giancarlo Aldini
    Istituto Chimico Farmaceutico Tossicologico, Faculty of Pharmacy, University of Milan, Viale Abruzzi 42, Milan 20131, Italy
    Biochem Biophys Res Commun 318:405-14. 2004
    ..Thus, in normoxic/physiological conditions, HbFe(II)NO does not induce significant NO-dependent vasorelaxation...
  22. doi request reprint Protein carbonylation: 2,4-dinitrophenylhydrazine reacts with both aldehydes/ketones and sulfenic acids
    Isabella Dalle-Donne
    Department of Biology, University of Milan, Italy
    Free Radic Biol Med 46:1411-9. 2009
    ..beta-Mercaptoethanol prevents the formation of the DNPH derivative because it reacts with the oxidized Cys residue, forming the corresponding disulfide...
  23. doi request reprint Design, synthesis, ADME properties, and pharmacological activities of β-alanyl-D-histidine (D-carnosine) prodrugs with improved bioavailability
    Marica Orioli
    Dipartimento di Scienze Farmaceutiche Pietro Pratesi, Universita degli Studi di Milano, Via Mangiagalli 25, 20133 Milan, Italy
    ChemMedChem 6:1269-82. 2011
    ....
  24. ncbi request reprint Water-Soluble alpha,beta-unsaturated aldehydes of cigarette smoke induce carbonylation of human serum albumin
    Graziano Colombo
    Dipartimento di Biologia, Universita degli Studi di Milano, Milan, Italy
    Antioxid Redox Signal 12:349-64. 2010
    ..Carbonylation of HSA associated with cigarette smoking might result in modifications of its antioxidant properties and transport functions of both endogenous and exogenous compounds...
  25. doi request reprint Fragmental modeling of hPepT2 and analysis of its binding features by docking studies and pharmacophore mapping
    Alessandro Pedretti
    Dipartimento di Scienze Farmaceutiche Pietro Pratesi, Facolta di Farmacia, Universita degli Studi di Milano, Via Mangiagalli, 25, I 20133 Milano, Italy
    Bioorg Med Chem 19:4544-51. 2011
    ....
  26. ncbi request reprint Actin Cys374 as a nucleophilic target of alpha,beta-unsaturated aldehydes
    Isabella Dalle-Donne
    Department of Biology, University of Milan, Via Celoria 26, I 20133 Milan, Italy
    Free Radic Biol Med 42:583-98. 2007
    ....
  27. ncbi request reprint Total antioxidant performance: a validated fluorescence assay for the measurement of plasma oxidizability
    Giangiacomo Beretta
    Istituto Chimico Farmaceutico Tossicologico, Faculty of Pharmacy, University of Milan, 20143 Milan, Italy
    Anal Biochem 354:290-8. 2006
    ..93, P<0.01). The results confirm that the TAP assay is suitable to measure the antioxidant activity of plasma antioxidants localized in both the lipophilic and hydrophilic compartments...
  28. doi request reprint Oxidative damage in human gingival fibroblasts exposed to cigarette smoke
    Graziano Colombo
    Department of Biology, Universita degli Studi di Milano, I 20133 Milan, Italy
    Free Radic Biol Med 52:1584-96. 2012
    ..GSH depletion could be a mechanism for cigarette smoke-induced cytotoxicity and could be correlated with the reduced reparative and regenerative activity of gingival and periodontal tissues previously reported in smokers...
  29. doi request reprint Design, synthesis, and evaluation of carnosine derivatives as selective and efficient sequestering agents of cytotoxic reactive carbonyl species
    Giulio Vistoli
    Dipartimento di Scienze Farmaceutiche Pietro Pratesi, Facolta di Farmacia, Universita degli Studi di Milano, Via Mangiagalli 25, 20133 Milano, Italy
    ChemMedChem 4:967-75. 2009
    ..This study describes a set of aryl derivatives that are characterized by high stability in human plasma and a quenching activity toward 4-hydroxy-trans-2-nonenal (HNE), chosen as a model of RCS, up to threefold greater than D-carnosine...
  30. ncbi request reprint Nitric oxide release and distribution following oral and intraperitoneal administration of nitroaspirin (NCX 4016) in the rat
    Marina Carini
    Istituto Chimico Farmaceutico Tossicologico, University of Milan, Viale Abruzzi 42, 20131 Milan, Italy
    Life Sci 74:3291-305. 2004
    ..This indicates that NCX 4016 does not undergo biotransformation in the upper part of gastrointestinal tract (no direct release of NO in this district) and that the stomach acts as a reservoir for the drug...
  31. ncbi request reprint LC-ESI-MS/MS determination of 4-hydroxy-trans-2-nonenal Michael adducts with cysteine and histidine-containing peptides as early markers of oxidative stress in excitable tissues
    Marica Orioli
    Istituto Chimico Farmaceutico Tossicologico, Faculty of Pharmacy, University of Milan, Viale Abruzzi 42, 20131 Milan, Italy
    J Chromatogr B Analyt Technol Biomed Life Sci 827:109-18. 2005
    ....
  32. ncbi request reprint Lipoxidation-derived reactive carbonyl species as potential drug targets in preventing protein carbonylation and related cellular dysfunction
    Giancarlo Aldini
    Institute of Pharmaceutical and Toxicological Chemistry Pietro Pratesi, Faculty of Pharmacy, University of Milan, Viale Abruzzi 42, 20131, Milan, Italy
    ChemMedChem 1:1045-58. 2006
  33. doi request reprint A combined high-resolution mass spectrometric and in silico approach for the characterisation of small ligands of beta2-microglobulin
    Luca Regazzoni
    Dipartimento di Scienze Farmaceutiche Pietro Pratesi, Universita degli Studi di Milano, Via Mangiagalli 25, Milan, Italy
    ChemMedChem 5:1015-25. 2010
    ..Overall, this approach can be used for predictive purposes and for a rapid screening of fibrillogenesis inhibitors...
  34. ncbi request reprint Chemiluminescence and LC-MS/MS analyses for the study of nitric oxide release and distribution following oral administration of nitroaspirin (NCX 4016) in healthy volunteers
    Marina Carini
    Istituto Chimico Farmaceutico Tossicologico, University of Milan, Viale Abruzzi 42, 20131 Milan, Italy
    J Pharm Biomed Anal 35:277-87. 2004
    ..0 +/- 0.6 h versus 5.4 +/- 1.2 h)...
  35. ncbi request reprint Edaravone inhibits protein carbonylation by a direct carbonyl-scavenging mechanism: focus on reactivity, selectivity, and reaction mechanisms
    Giancarlo Aldini
    Dipartimento di Scienze Farmaceutiche Pietro Pratesi, Universita degli Studi di Milano, Milan, Italy
    Antioxid Redox Signal 12:381-92. 2010
    ..The same combined approach indicated that EDA is not a selective RCS scavenger, being able to react also with nontoxic, physiologically relevant aldehydes, such as pyridoxal...
  36. doi request reprint Exploring the space of histidine containing dipeptides in search of novel efficient RCS sequestering agents
    Giulio Vistoli
    Dipartimento di Scienze Farmaceutiche, Universita degli Studi di Milano, Via Mangiagalli, 25, I 20133 Milano, Italy
    Eur J Med Chem 66:153-60. 2013
    ....
  37. ncbi request reprint Antioxidant and photoprotective activity of a lipophilic extract containing neolignans from Krameria triandra roots
    Marina Carini
    Istituto Chimico Farmaceutico Tossicologico, University of Milan, Italy
    Planta Med 68:193-7. 2002
    ..The results of this study indicate the potential use of Rhatany extracts, standardized in neolignans, as topical antioxidants/radical scavengers against skin photodamage...
  38. ncbi request reprint HNE Michael adducts to histidine and histidine-containing peptides as biomarkers of lipid-derived carbonyl stress in urines: LC-MS/MS profiling in Zucker obese rats
    Marica Orioli
    Istituto di Chimica Farmaceutica e Tossicologica Pietro Pratesi, Faculty of Pharmacy, University of Milan, Via Mangiagalli 25, I 20133 Milan, Italy
    Anal Chem 79:9174-84. 2007
    ....
  39. ncbi request reprint (-)-Epigallocatechin-3-gallate prevents oxidative damage in both the aqueous and lipid compartments of human plasma
    Giancarlo Aldini
    Istituto Chimico Farmaceutico Tossicologico, University of Milan, Milan, Italy
    Biochem Biophys Res Commun 302:409-14. 2003
    ..Further, ESR experiments confirmed that EGCG recycled alpha-tocopherol through a H-transfer mechanism at the aqueous/lipid interface affording an additional protective mechanism to the lipid compartment of plasma...
  40. doi request reprint Protein modification by acrolein: relevance to pathological conditions and inhibition by aldehyde sequestering agents
    Giancarlo Aldini
    Department of Pharmaceutical Sciences Pietro Pratesi, Universita degli Studi di Milano, Milan, Italy
    Mol Nutr Food Res 55:1301-19. 2011
    ....
  41. doi request reprint Modeling of the intestinal peptide transporter hPepT1 and analysis of its transport capacities by docking and pharmacophore mapping
    Alessandro Pedretti
    Istituto di Chimica Farmaceutica e Tossicologica Pietro Pratesi, Facolta di Farmacia, Universita degli Studi di Milano, Via Mangiagalli 25, 20133 Milano, Italy
    ChemMedChem 3:1913-21. 2008
    ....
  42. doi request reprint Hemoglobin glutathionylation can occur through cysteine sulfenic acid intermediate: electrospray ionization LTQ-Orbitrap hybrid mass spectrometry studies
    Luca Regazzoni
    Dipartimento di Scienze Farmaceutiche Pietro Pratesi, Universita degli Studi di Milano, Milan, Italy
    J Chromatogr B Analyt Technol Biomed Life Sci 877:3456-61. 2009
    ..Hb glutathionylation is also mediated by a thiol-disulfide transfer mechanism, but this requires a concentration of GSSG which is far to be achieved in physiopathological conditions...
  43. ncbi request reprint Acrolein-sequestering ability of endogenous dipeptides: characterization of carnosine and homocarnosine/acrolein adducts by electrospray ionization tandem mass spectrometry
    Marina Carini
    Istituto Chimico Farmaceutico Tossicologico, University of Milan, Viale Abruzzi 42, 20131 Milan, Italy
    J Mass Spectrom 38:996-1006. 2003
    ....
  44. ncbi request reprint Pathophysiology of tobacco smoke exposure: recent insights from comparative and redox proteomics
    Graziano Colombo
    Department of Biosciences, Universita degli Studi di Milano, Milan, Italy
    Mass Spectrom Rev 33:183-218. 2014
    ....
  45. doi request reprint Transforming dietary peptides in promising lead compounds: the case of bioavailable carnosine analogs
    Giulio Vistoli
    Department of Pharmaceutical Sciences Pietro Pratesi, Universita degli Studi di Milano, Via Mangiagalli 25, 20133 Milan, Italy
    Amino Acids 43:111-26. 2012
    ..The review also includes a brief description of AGEs and ALEs as drug targets and the evidence so far reported regarding the ability of carnosine as inhibitor of AGEs and ALEs formation and the proposed reaction mechanisms...
  46. ncbi request reprint In vitro metabolism of a nitroderivative of acetylsalicylic acid (NCX4016) by rat liver: LC and LC-MS studies
    Marina Carini
    Istituto Chimico Farmaceutico Tossicologico, Viale Abruzzi 42, Milan, Italy
    J Pharm Biomed Anal 29:1061-71. 2002
    ..The relevance of this new metabolic pathway in NCX4016 detoxification by rat liver is discussed...
  47. doi request reprint A rapid and sensitive LC-ESI-MS/MS method for detection and quantitation of methylprednisolone and methylprednisolone acetate in rat plasma after intra-articular administration
    Alessia Panusa
    Dipartimento di Scienze Farmaceutiche P Pratesi, Universita degli Studi di Milano, 20133 Milan, Italy
    J Pharm Biomed Anal 51:691-7. 2010
    ..080 and 0.072pmol injected for MP and MPA, respectively). The method was successfully applied to monitor the plasma levels of MPA and MP following intra-articular (IA) injections of a low MPA (Depo-Medrol((R))) dose in rats...
  48. ncbi request reprint A sensitive and specific precursor ion scanning approach in liquid chromatography/electrospray ionization tandem mass spectrometry to detect methylprednisolone acetate and its metabolites in rat urine
    Alessia Panusa
    Dipartimento di Scienze Farmaceutiche Pietro Pratesi, Universita degli Studi di Milano, Via L Mangiagalli 25, 20133 Milan, Italy
    Rapid Commun Mass Spectrom 24:1583-94. 2010
    ..Six different metabolites were detected in rat urine, and their chemical structures were assigned with a computational study...
  49. ncbi request reprint A novel high resolution MS approach for the screening of 4-hydroxy-trans-2-nonenal sequestering agents
    Mara Colzani
    Department of Pharmaceutical Sciences, Universita degli Studi di Milano, Via Mangiagalli 25, 20133 Milan, Italy Electronic address
    J Pharm Biomed Anal 91:108-18. 2014
    ..In summary, this paper reports a validated and highly reproducible MS method to test the ability of pure compounds as well as natural extracts to act as protein carbonylation inhibitors. ..
  50. ncbi request reprint Lemon peel and Limoncello liqueur: a proteomic duet
    Elisa Fasoli
    Department of Chemistry, Materials and Chemical Engineering, Politecnico di Milano, Milan, Italy
    Biochim Biophys Acta 1834:1484-91. 2013
    ..This could be the starting point for investigating the genuineness and natural origin of commercial drinks in order to protect consumers from adulterated products. ..
  51. doi request reprint The potential of resveratrol against human gliomas
    Nicoletta Gagliano
    Department of Human Morphology and Biomedical Sciences Citta Studi, Universita di Milano, School of Medicine, Extracellular Matrix Laboratory, Via Fratelli Cervi 93, 20090 Segrate, Milan, Italy
    Anticancer Drugs 21:140-50. 2010
    ..This study discusses current knowledge with regard to the effects of resveratrol in relation to its potential as a chemopreventive and/or chemotherapeutic molecule against human gliomas...
  52. ncbi request reprint Protein carbonylation, cellular dysfunction, and disease progression
    Isabella Dalle-Donne
    Department of Biology, University of Milan, Italy
    J Cell Mol Med 10:389-406. 2006
    ....
  53. ncbi request reprint Homology modeling of human serum carnosinase, a potential medicinal target, and MD simulations of its allosteric activation by citrate
    Giulio Vistoli
    Istituto di Chimica Farmaceutica, Facolta di Farmacia, Universita di Milano, Viale Abruzzi 42, I 20131 Milano, Italy
    J Med Chem 49:3269-77. 2006
    ..e., binding score) of carnosine to the catalytic site. This is one of the first reports documenting the molecular mechanism of an allosteric enzyme activator using MD simulations...
  54. ncbi request reprint Mass spectrometric approaches for the identification and quantification of reactive carbonyl species protein adducts
    Mara Colzani
    Department of Pharmaceutical Sciences, Universita degli Studi di Milano, Milan, Italy
    J Proteomics 92:28-50. 2013
    ..This review focuses also on the different MS-based approaches so far developed for RCS-protein adduct quantification. This article is part of a Special Issue entitled: Posttranslational Protein modifications in biology and Medicine. ..
  55. doi request reprint Molecular strategies to prevent, inhibit, and degrade advanced glycoxidation and advanced lipoxidation end products
    Giancarlo Aldini
    Department of Pharmaceutical Sciences, University of Milan, Milan, Italy
    Free Radic Res 47:93-137. 2013
    ..The review discusses inhibitors of ALE and AGE formation, cross-link breakers, ALE/AGE elimination by enzymes and proteolytic systems, receptors for advanced glycation end products (RAGEs) and blockade of the ligand-RAGE axis...
  56. doi request reprint Screening of fibrillogenesis inhibitors of β2-microglobulin: integrated strategies by mass spectrometry capillary electrophoresis and in silico simulations
    Luca Regazzoni
    Dipartimento di Scienze Farmaceutiche Pietro Pratesi, Universita degli Studi di Milano, Via Mangiagalli 25, 20133 Milan, Italy
    Anal Chim Acta 685:153-61. 2011
    ..Studies on the new hit thus retrieved confirm the predictive potential of both the equation on affinity and of docking analysis on anti-amyloid activity...
  57. ncbi request reprint Mass spectrometry for detection of 4-hydroxy-trans-2-nonenal (HNE) adducts with peptides and proteins
    Marina Carini
    Istituto Chimico Farmaceutico Tossicologico, Faculty of Pharmacy, University of Milan, Viale Abruzzi 42, 20131 Milan, Italy
    Mass Spectrom Rev 23:281-305. 2004
    ....
  58. pmc D-Carnosine octylester attenuates atherosclerosis and renal disease in ApoE null mice fed a Western diet through reduction of carbonyl stress and inflammation
    Stefano Menini
    Department of Clinical and Molecular Medicine, La Sapienza University of Rome, Rome, Italy
    Br J Pharmacol 166:1344-56. 2012
    ..This study aimed at assessing the effect of the carnosinase-resistant, D-carnosine, on HNE-induced cellular injury and of its bioavailable prodrug D-carnosine octylester on experimental atherosclerosis and renal disease...
  59. ncbi request reprint Mass spectrometric strategies and their applications for molecular mass determination of recombinant therapeutic proteins
    Marina Carini
    Department of Pharmaceutical Sciences Pietro Pratesi, Universita degli Studi di Milano, Milan, Italy
    Curr Pharm Biotechnol 12:1548-57. 2011
    ..The paper is a review of the most used and advanced MS strategies so far reported as well as of their applications in the biopharmaceutical field...
  60. doi request reprint En bloc elution of proteomes from combinatorial peptide ligand libraries
    Alessia Farinazzo
    Department of Chemistry, Materials and Chemical Engineering Giulio Natta, Politecnico di Milano, Via Mancinelli 7, Milan, Italy
    J Proteomics 72:725-30. 2009
    ....
  61. doi request reprint Molecular recognition of T:G mismatched base pairs in DNA as studied by electrospray ionization mass spectrometry
    Federico Riccardi Sirtori
    Oncology Business Unit, Nerviano Medical Sciences, Viale Pasteur 10, 20014 Nerviano MI, Italy
    ChemMedChem 7:1112-22. 2012
    ..Our results confirm that this ESI method could be a valuable tool for the identification of new molecules able to specifically recognize T:G mismatched base pairs...
  62. ncbi request reprint Modification of lymphocyte DNA damage by carotenoid supplementation in postmenopausal women
    Xianfeng Zhao
    Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, Boston, MA 02111, USA
    Am J Clin Nutr 83:163-9. 2006
    ..Carotenoids could be a part of a protective strategy to minimize oxidative damage in vulnerable populations, such as the elderly...
  63. ncbi request reprint S-nitrosation versus S-glutathionylation of protein sulfhydryl groups by S-nitrosoglutathione
    Daniela Giustarini
    Department of Neuroscience, Pharmacology Unit, University of Siena, Siena, Italy
    Antioxid Redox Signal 7:930-9. 2005
    ..The susceptibility of the modified proteins to denitrosation and deglutathionylation by reduced glutathione was also investigated...
  64. ncbi request reprint The activities of antioxidant nutrients in human plasma depend on the localization of attacking radical species
    Kyung Jin Yeum
    The Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, Boston, MA, USA
    J Nutr 133:2688-91. 2003
    ..Our study indicates that the antioxidant nutrients present in both the lipid and aqueous compartments can remove free radicals generated in plasma, and their activity depends on the localization of the attacking radical species...
  65. pmc Efficacy and age-related effects of nitric oxide-releasing aspirin on experimental restenosis
    Claudio Napoli
    Department of Medicine, Federico II University of Naples, 80131 Naples, Italy
    Proc Natl Acad Sci U S A 99:1689-94. 2002
    ..We suggest that an NCX-4016 derivative could be an effective drug in reducing restenosis, especially in the presence of aging and/or gastrointestinal damage...
  66. ncbi request reprint Effect of a standardized grape seed extract on low-density lipoprotein susceptibility to oxidation in heavy smokers
    Giovanni B Vigna
    Department of Clinical and Experimental Medicine, Section of Internal Medicine II, University of Ferrara, Ferrara, Italy
    Metabolism 52:1250-7. 2003
    ..The antioxidant potential of grape seed extract polyphenols may prove effective in a model of oxidative stress (smoking); however more investigational data are needed before use in wider clinical settings...
  67. ncbi request reprint Biomarkers of antioxidant capacity in the hydrophilic and lipophilic compartments of human plasma
    Kyung Jin Yeum
    The Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, Boston, MA 02111, USA
    Arch Biochem Biophys 430:97-103. 2004
    ..Revelation of the mechanism of action of antioxidants and their true antioxidant potential will help us to optimize the antioxidant defenses in the body...