Lilia Alberghina

Summary

Affiliation: University of Milano-Bicocca
Country: Italy

Publications

  1. pmc Recombinant human nerve growth factor with a marked activity in vitro and in vivo
    Anna M Colangelo
    Laboratory of Neuroscience R Levi Montalcini, Department of Biotechnology and Biosciences, University of Milano Bicocca, Milan, Italy
    Proc Natl Acad Sci U S A 102:18658-63. 2005
  2. doi request reprint Cell growth and cell cycle in Saccharomyces cerevisiae: basic regulatory design and protein-protein interaction network
    Lilia Alberghina
    Dipartimento di Biotecnologie e Bioscienze, Universita di Milano Bicocca, Milano, Italy
    Biotechnol Adv 30:52-72. 2012
  3. pmc Towards a systems biology approach to mammalian cell cycle: modeling the entrance into S phase of quiescent fibroblasts after serum stimulation
    Roberta Alfieri
    Institute for Biomedical Technology Consiglio Nazionale delle Ricerche, Via Fratelli Cervi 93, Segrate, Milan, Italy
    BMC Bioinformatics 10:S16. 2009
  4. ncbi request reprint Checking cell size in budding yeast: a systems biology approach
    Lilia Alberghina
    Dipartimento di Biotecnologie e Bioscienze, Universita di Milano Bicocca, Milano Italy
    Ital J Biochem 52:55-7. 2003
  5. pmc The modular systems biology approach to investigate the control of apoptosis in Alzheimer's disease neurodegeneration
    Lilia Alberghina
    Department of Biotechnology and Biosciences, Laboratory of Neuroscience R Levi Montalcini, University of Milano Bicocca, 20126 Milan, Italy
    BMC Neurosci 7:S2. 2006
  6. doi request reprint Proteomics and systems biology to tackle biological complexity: Yeast as a case study
    Lilia Alberghina
    Department of Biotechnology and Biosciences, University of Milano Bicocca, Milano, Italy
    Proteomics 10:4337-41. 2010
  7. doi request reprint Systems biology of the cell cycle of Saccharomyces cerevisiae: From network mining to system-level properties
    Lilia Alberghina
    Department of Biotechnology and Biosciences, University of Milano Bicocca, P zza della Scienza 2, 20126 Milano, Italy
    Biotechnol Adv 27:960-78. 2009
  8. ncbi request reprint Systems biology and the molecular circuits of cancer
    Lilia Alberghina
    Department of Biotechnology and Biosciences, Universiy of Milano Bicocca, Piazza della Scienza 2, 20126 Milano, Italy
    Chembiochem 5:1322-33. 2004
  9. doi request reprint Molecular networks and system-level properties
    Lilia Alberghina
    Department of Biotechnology and Biosciences, University of Milano Bicocca, P zza della Scienza 2, 20126 Milano, Italy
    J Biotechnol 144:224-33. 2009
  10. pmc A cell sizer network involving Cln3 and Far1 controls entrance into S phase in the mitotic cycle of budding yeast
    Lilia Alberghina
    Department of Biotechnology and Biosciences, University of Milano Bicocca, 20126 Milan, Italy
    J Cell Biol 167:433-43. 2004

Collaborators

Detail Information

Publications56

  1. pmc Recombinant human nerve growth factor with a marked activity in vitro and in vivo
    Anna M Colangelo
    Laboratory of Neuroscience R Levi Montalcini, Department of Biotechnology and Biosciences, University of Milano Bicocca, Milan, Italy
    Proc Natl Acad Sci U S A 102:18658-63. 2005
    ..This work describes the production on a laboratory scale of high levels of a rhNGF with in vitro and, more important, in vivo biological activity equivalent to the native murine protein...
  2. doi request reprint Cell growth and cell cycle in Saccharomyces cerevisiae: basic regulatory design and protein-protein interaction network
    Lilia Alberghina
    Dipartimento di Biotecnologie e Bioscienze, Universita di Milano Bicocca, Milano, Italy
    Biotechnol Adv 30:52-72. 2012
    ....
  3. pmc Towards a systems biology approach to mammalian cell cycle: modeling the entrance into S phase of quiescent fibroblasts after serum stimulation
    Roberta Alfieri
    Institute for Biomedical Technology Consiglio Nazionale delle Ricerche, Via Fratelli Cervi 93, Segrate, Milan, Italy
    BMC Bioinformatics 10:S16. 2009
    ....
  4. ncbi request reprint Checking cell size in budding yeast: a systems biology approach
    Lilia Alberghina
    Dipartimento di Biotecnologie e Bioscienze, Universita di Milano Bicocca, Milano Italy
    Ital J Biochem 52:55-7. 2003
    ..A systems biology-based approach, that is needed to structure the underlying complexity of cell cycle regulatory mechanisms, is described...
  5. pmc The modular systems biology approach to investigate the control of apoptosis in Alzheimer's disease neurodegeneration
    Lilia Alberghina
    Department of Biotechnology and Biosciences, Laboratory of Neuroscience R Levi Montalcini, University of Milano Bicocca, 20126 Milan, Italy
    BMC Neurosci 7:S2. 2006
    ..Moreover, after giving an overview of the many molecular mechanisms underlying apoptosis in AD, we present both a modular and a molecular model of neuronal apoptosis that suggest new insights on neuroprotection for this disease...
  6. doi request reprint Proteomics and systems biology to tackle biological complexity: Yeast as a case study
    Lilia Alberghina
    Department of Biotechnology and Biosciences, University of Milano Bicocca, Milano, Italy
    Proteomics 10:4337-41. 2010
    ....
  7. doi request reprint Systems biology of the cell cycle of Saccharomyces cerevisiae: From network mining to system-level properties
    Lilia Alberghina
    Department of Biotechnology and Biosciences, University of Milano Bicocca, P zza della Scienza 2, 20126 Milano, Italy
    Biotechnol Adv 27:960-78. 2009
    ..The relevance of the insight coming from the cell cycle SB studies in developing a new framework for tackling very complex biological processes, such as cancer and aging, is discussed...
  8. ncbi request reprint Systems biology and the molecular circuits of cancer
    Lilia Alberghina
    Department of Biotechnology and Biosciences, Universiy of Milano Bicocca, Piazza della Scienza 2, 20126 Milano, Italy
    Chembiochem 5:1322-33. 2004
    ..Extension of this hierarchical, modular approach to tumors as a whole holds promise for the development of effective drug discovery approaches and more efficient therapeutic protocols...
  9. doi request reprint Molecular networks and system-level properties
    Lilia Alberghina
    Department of Biotechnology and Biosciences, University of Milano Bicocca, P zza della Scienza 2, 20126 Milano, Italy
    J Biotechnol 144:224-33. 2009
    ..Furthermore, the principles outlined herein may provide new insight to network analysis in drug discovery...
  10. pmc A cell sizer network involving Cln3 and Far1 controls entrance into S phase in the mitotic cycle of budding yeast
    Lilia Alberghina
    Department of Biotechnology and Biosciences, University of Milano Bicocca, 20126 Milan, Italy
    J Cell Biol 167:433-43. 2004
    ..We show that a second threshold is required together with the Cln3/Far1 threshold for carbon source modulation of Ps. A new molecular network accounting for the setting of Ps is proposed...
  11. ncbi request reprint The CK2 phosphorylation of catalytic domain of Cdc34 modulates its activity at the G1 to S transition in Saccharomyces cerevisiae
    Paola Coccetti
    Department of Biotechnology and Biosciences, University of Milano Bicocca, Milano, Italy
    Cell Cycle 7:1391-401. 2008
    ....
  12. doi request reprint Overexpression of Far1, a cyclin-dependent kinase inhibitor, induces a large transcriptional reprogramming in which RNA synthesis senses Far1 in a Sfp1-mediated way
    Stefano Busti
    Dipartimento di Biotecnologie e Bioscienze, Universita di Milano Bicocca, Milano, Italy
    Biotechnol Adv 30:185-201. 2012
    ....
  13. doi request reprint Sir2-dependent asymmetric segregation of damaged proteins in ubp10 null mutants is independent of genomic silencing
    Ivan Orlandi
    Dipartimento di Biotecnologie e Bioscienze, Universita degli Studi di Milano Bicocca, 20126 Milano, Italy
    Biochim Biophys Acta 1803:630-8. 2010
    ....
  14. ncbi request reprint Sic1 is phosphorylated by CK2 on Ser201 in budding yeast cells
    Paola Coccetti
    Dipartimento di Biotecnologie e Bioscienze, Universita degli Studi Milano Bicocca, P zza della Scienza 2, 20126 Milano, Italy
    Biochem Biophys Res Commun 346:786-93. 2006
    ..These data strongly support the notion that CK2 phosphorylates Sic1 in vivo...
  15. ncbi request reprint Involvement of the yeast metacaspase Yca1 in ubp10Delta-programmed cell death
    Maurizio Bettiga
    Dipartimento di Biotecnologie e Bioscienze, Universita degli Studi di Milano Bicocca, Piazza della Scienza 2, 20126 Milan, Italy
    FEMS Yeast Res 5:141-7. 2004
    ..All these results argue in favour of an involvement of the yeast metacaspase in the active cell death triggered by loss of UBP10 function...
  16. doi request reprint Snf1/AMPK promotes SBF and MBF-dependent transcription in budding yeast
    Sara Busnelli
    SYSBIO, Centre of Systems Biology, Milan, Italy Department of Biotechnology and Biosciences, University of Milano Bicocca, P zza della Scienza 2, 20126 Milan, Italy
    Biochim Biophys Acta 1833:3254-64. 2013
    ....
  17. doi request reprint Novel RasGRF1-derived Tat-fused peptides inhibiting Ras-dependent proliferation and migration in mouse and human cancer cells
    Elena Sacco
    Department of Biotechnology and Biosciences, University of Milano Bicocca, Milano, Italy
    Biotechnol Adv 30:233-43. 2012
    ..These results support the use of RasGRF1-derived peptides as model compounds for the development of Ras inhibitory anticancer agents...
  18. pmc Oncogenic K-Ras decouples glucose and glutamine metabolism to support cancer cell growth
    Daniela Gaglio
    Department of Biotechnology and Biosciences, University of Milano Bicocca, Milan, Italy
    Mol Syst Biol 7:523. 2011
    ..These results provide evidence for a role of oncogenic K-Ras in the metabolic reprogramming of cancer cells...
  19. doi request reprint A surface-activated chemical ionization approach allows quantitative phosphorylation analysis of the cyclin-dependent kinase inhibitor Sic1 phosphorylated on Ser201
    Claudia Cirulli
    Universita degli Studi di Milano Bicocca, Dipartimento di Biotecnologie e Bioscienze, Piazza della Scienza 2, 20126 Milano, Italy
    Rapid Commun Mass Spectrom 26:1527-32. 2012
    ..The main advantages of SACI-MS technology are high sensitivity, quantitative accuracy and matrix effect reduction, which allow quantitative estimations...
  20. ncbi request reprint Snf1/AMPK promotes S-phase entrance by controlling CLB5 transcription in budding yeast
    Stefania Pessina
    Department of Biotechnology and Biosciences, University of Milano Bicocca, Milano, Italy
    Cell Cycle 9:2189-200. 2010
    ..In conclusion, this report describes a previously unrecognized role for Snf1 in transcriptional modulation of the G 1 to S transition, differing from the reported AMPK role in controlling the G 1/S transition in multicellular eukaryotes...
  21. doi request reprint CK2 activity is modulated by growth rate in Saccharomyces cerevisiae
    Farida Tripodi
    Dipartimento di Biotecnologie e Bioscienze, Universita di Milano Bicocca, Piazza della Scienza 2, 20126 Milano, Italy
    Biochem Biophys Res Commun 398:44-50. 2010
    ..Also in chemostat cultures nuclear CK2 activity is higher in faster growing cells providing the first unequivocal demonstration that growth rate itself can affect CK2 activity in a eukaryotic organism...
  22. pmc An acidic loop and cognate phosphorylation sites define a molecular switch that modulates ubiquitin charging activity in Cdc34-like enzymes
    Elena Papaleo
    Department of Biotechnology and Biosciences, University of Milano Bicocca, Milan, Italy
    PLoS Comput Biol 7:e1002056. 2011
    ....
  23. ncbi request reprint In CK2 inactivated cells the cyclin dependent kinase inhibitor Sic1 is involved in cell-cycle arrest before the onset of S phase
    Farida Tripodi
    Dipartimento di Biotecnologie e Bioscienze, Universita Milano Bicocca, P zza della Scienza 2, 20126 Milano, Italy
    Biochem Biophys Res Commun 359:921-7. 2007
    ..These findings indicate that Sic1 plays a major role during G1 arrest of CK2-inactivated cells...
  24. ncbi request reprint Rapamycin-mediated G1 arrest involves regulation of the Cdk inhibitor Sic1 in Saccharomyces cerevisiae
    Vittoria Zinzalla
    Department of Biotechnology and Biosciences, University of Milano Bicocca, Piazza della Scienza 2, 20126, Milano, Italy
    Mol Microbiol 63:1482-94. 2007
    ....
  25. ncbi request reprint SFP1 is involved in cell size modulation in respiro-fermentative growth conditions
    Chiara Cipollina
    Dipartimento di Biotecnologie e Bioscienze, Universita degli Studi di Milano Bicocca, Piazza della Scienza 2, 20126, Milano, Italy
    Yeast 22:385-99. 2005
    ..These features, combined with the role of Sfp1p as a transcriptional factor, suggest that Sfp1p could be an important element in the control of the cell size modulated by nutrients...
  26. ncbi request reprint CK2 regulates in vitro the activity of the yeast cyclin-dependent kinase inhibitor Sic1
    Matteo Barberis
    Dipartimento di Biotecnologie e Bioscienze, Universita degli Studi di Milano Bicocca, Italy
    Biochem Biophys Res Commun 336:1040-8. 2005
    ..Taken together, these data disclose the possibility that CK2 plays a role in the regulation of Sic1 activity...
  27. ncbi request reprint Proteomic analysis of a nutritional shift-up in Saccharomyces cerevisiae identifies Gvp36 as a BAR-containing protein involved in vesicular traffic and nutritional adaptation
    Lorenzo Querin
    Department of Biotechnology and Biosciences, University Milano Bicocca, 20126 Milano, Italy
    J Biol Chem 283:4730-43. 2008
    ..We thus propose that Gvp36 represents a novel yeast BAR protein involved in vesicular traffic and in nutritional adaptation...
  28. ncbi request reprint Mutations of the CK2 phosphorylation site of Sic1 affect cell size and S-Cdk kinase activity in Saccharomyces cerevisiae
    Paola Coccetti
    Dipartimento di Biotecnologie e Bioscienze, Universita degli Studi Milano Bicocca, P zza della Scienza 2, 20126 Milano, Italy
    Mol Microbiol 51:447-60. 2004
    ..A strong reduction of the same parameters is observed in cells harbouring Sic1S201E. The deregulated coordination between cell size and cell cycle is also apparent at the level of S-Cdk activity...
  29. ncbi request reprint Subcellular localization of the cyclin dependent kinase inhibitor Sic1 is modulated by the carbon source in budding yeast
    Riccardo L Rossi
    Department of Biotechnology and Biosciences, University of Milano Bicocca, Milan, Italy
    Cell Cycle 4:1798-807. 2005
    ..Our data indicate a previously unrecognized inhibitor/activator dual role for Sic1 and put it among key molecules whose activity is regulated by their nuclear-cytoplasmic localization...
  30. pmc Glutamine deprivation induces abortive s-phase rescued by deoxyribonucleotides in k-ras transformed fibroblasts
    Daniela Gaglio
    Department of Biotechnology and Biosciences, University of Milano Bicocca, Milan, Italy
    PLoS ONE 4:e4715. 2009
    ..Another metabolic adaptation of cancer cells, that has long been established, is their propensity to exhibit increased glutamine consumption, although the effects induced by glutamine deprivation on cancer cells are still controversial...
  31. ncbi request reprint Expression of transforming K-Ras oncogene affects mitochondrial function and morphology in mouse fibroblasts
    Ferdinando Chiaradonna
    Department of Biotechnology and Biosciences, University of Milano Bicocca, Piazza della Scienza 2, 20126 Milan, Italy
    Biochim Biophys Acta 1757:1338-56. 2006
    ....
  32. pmc The histone deubiquitinating enzyme Ubp10 is involved in rDNA locus control in Saccharomyces cerevisiae by affecting Sir2p association
    Luciano Calzari
    Dipartimento di Biotecnologie e Bioscienze, Universita degli Studi di Milano Bicocca, 20126 Milano, Italy
    Genetics 174:2249-54. 2006
    ..We show here that the histone deubiquitinating enzyme Ubp10 contributes to the formation/maintenance of silenced chromatin at the rDNA by affecting Sir2p association...
  33. pmc Cell size at S phase initiation: an emergent property of the G1/S network
    Matteo Barberis
    Department of Biotechnology and Biosciences, University of Milano Bicocca, Milan, Italy
    PLoS Comput Biol 3:e64. 2007
    ..Sensitivity analysis of PS provides a novel relevant conclusion: PS is an emergent property of the G1 to S network that strongly depends on growth rate...
  34. pmc Glucose signaling-mediated coordination of cell growth and cell cycle in Saccharomyces cerevisiae
    Stefano Busti
    Dipartimento di Biotecnologie e Bioscienze, Universita di Milano Bicocca, Piazza della Scienza, 2 20126 Milano, Italy
    Sensors (Basel) 10:6195-240. 2010
    ..Many interconnections exist between the diverse glucose sensing systems, which enables yeast cells to fine tune cell growth, cell cycle and their coordination in response to nutritional changes...
  35. doi request reprint Regulation of hSos1 activity is a system-level property generated by its multi-domain structure
    Elena Sacco
    Department of Biotechnology and Biosciences, University of Milano Bicocca, Milano, Italy
    Biotechnol Adv 30:154-68. 2012
    ....
  36. ncbi request reprint Transcriptional profiling of ubp10 null mutant reveals altered subtelomeric gene expression and insurgence of oxidative stress response
    Ivan Orlandi
    Universita degli Studi di Milano Bicocca, Dipartimento di Biotecnologie e Bioscienze, Piazza della Scienza 2, 20126 Milano, Italy
    J Biol Chem 279:6414-25. 2004
    ..These results argue in favor of an involvement of deubiquitination in transcriptional control and suggest a linkage between oxidative stress and apoptotic pathway in budding yeast...
  37. pmc Nutritional limitation sensitizes mammalian cells to GSK-3β inhibitors and leads to growth impairment
    Paola de Candia
    Department of Biotechnology and Biosciences, University of Milano Bicocca, Milan, Italy
    Am J Pathol 178:1814-23. 2011
    ..These findings thus provide a rationale to further analyze the biochemical bases for combined glucose deprivation and GSK-3β inhibition as a new approach to control transformed cell growth...
  38. pmc Compaction properties of an intrinsically disordered protein: Sic1 and its kinase-inhibitor domain
    Stefania Brocca
    Department of Biotechnology and Biosciences, University of Milano Bicocca, Milan, Italy
    Biophys J 100:2243-52. 2011
    ..Comparison between the fragment and the full-length protein suggests that chain length is crucial to the stabilization of compact states of this IDP. The two proteins are compared by a length-independent compaction index...
  39. pmc The yeast cyclin-dependent kinase inhibitor Sic1 and mammalian p27Kip1 are functional homologues with a structurally conserved inhibitory domain
    Matteo Barberis
    Dipartimento di Biotecnologie e Bioscienze, Universita degli Studi di Milano Bicocca, Piazza della Scienza 2, 20126 Milano, Italy
    Biochem J 387:639-47. 2005
    ..Taken together, these findings strongly indicate that budding yeast Sic1 and mammalian p27(Kip1) are functional homologues with a structurally conserved inhibitory domain...
  40. ncbi request reprint The isolated catalytic hairpin of the Ras-specific guanine nucleotide exchange factor Cdc25Mm retains nucleotide dissociation activity but has impaired nucleotide exchange activity
    Elena Sacco
    Dipartimento di Biotechnologie e Bioscienze, Universita degli Studi di Milano Bicocca, Piazza della Scienza 2, 20126 Milano, Italy
    FEBS Lett 579:6851-8. 2005
    ..These results indicate that nucleotide re-entry and Ras/GEF dissociation--final steps in the GEF catalytic cycle--require GEF regions different from the HI hairpin. GEF down-sizing could lead to development of novel Ras inhibitors...
  41. doi request reprint Analysis and modeling of growing budding yeast populations at the single cell level
    Danilo Porro
    Department of Biotechnology and Biosciences, University of Milano Bicocca, Milan 20126, Italy
    Cytometry A 75:114-20. 2009
    ..Literature on the structure of growing yeast populations and modeling of cell cycle progression is reviewed...
  42. ncbi request reprint Catalytic competence of the Ras-GEF domain of hSos1 requires intra-REM domain interactions mediated by phenylalanine 577
    Elena Sacco
    Dipartimento di Biotecnologie e Bioscienze, Universita degli Studi di Milano Bicocca, Piazza della Scienza 2, 20126 Milano, Italy
    FEBS Lett 580:6322-8. 2006
    ..We show that an intra-REM domain interaction, mediated by phenylalanine 577, is required to allow interaction of the REM domain with the catalytic core, constraining it in the active conformation...
  43. pmc Data recovery and integration from public databases uncovers transformation-specific transcriptional downregulation of cAMP-PKA pathway-encoding genes
    Chiara Balestrieri
    Department of Biotechnology and Biosciences, University of Milano Bicocca, Piazza della Scienza 2, Milan, Italy
    BMC Bioinformatics 10:S1. 2009
    ....
  44. doi request reprint Unscrambling thermal stability and temperature adaptation in evolved variants of a cold-active lipase
    Pietro Gatti-Lafranconi
    Dipartimento di Biotecnologie e Bioscienze, Universita degli Studi di Milano Bicocca, Piazza della Scienza 2, Milan, Italy
    FEBS Lett 582:2313-8. 2008
    ..Biophysical measurements did not indicate any obvious difference between the improved variant and the wild type enzyme in terms of loss of secondary structure upon heat treatment, nor a shift in the apparent melting temperature...
  45. doi request reprint Electrospray ionization-mass spectrometry conformational analysis of isolated domains of an intrinsically disordered protein
    Lorenzo Testa
    Department of Biotechnology and Bioscience, University of Milano Bicocca, Milan, Italy
    Biotechnol J 6:96-100. 2011
    ..The results are consistent with previously reported differences between the two protein moieties and corroborate the feasibility of IDP conformational analysis by ESI-MS...
  46. ncbi request reprint Heterologous production of five Hepatitis C virus-derived antigens in three Saccharomyces cerevisiae host strains
    Carola Parolin
    Universita degli Studi di Milano Bicocca, Dipartimento di Biotecnologie e Bioscienze, P za della Scienza 2, 20126 Milano, Italy
    J Biotechnol 120:46-58. 2005
    ..For every recombinant antigen tested, the highest production levels were achieved with the CENPK 113-5D-host strain, while the GRF18U strain shows symptoms of a heavily stressed phenotype...
  47. pmc Order propensity of an intrinsically disordered protein, the cyclin-dependent-kinase inhibitor Sic1
    Stefania Brocca
    Department of Biotechnology and Biosciences, University of Milano Bicocca, Milan, Italy
    Proteins 76:731-46. 2009
    ..The mutations S201A and S201E, which are known to affect Sic1 function, do not have significant effects on the conformational properties of the pure protein...
  48. pmc Cancer cell growth and survival as a system-level property sustained by enhanced glycolysis and mitochondrial metabolic remodeling
    Lilia Alberghina
    SysBio Centre for Systems Biology Milano and Rome, Italy Department of Biotechnology and Biosciences, University of Milano Bicocca, Piazza della Scienza Milano, Italy
    Front Physiol 3:362. 2012
    ..Taken together the findings reported herein indicate that a system-level property is sustained by a cascade of interconnected biochemical pathways that behave differently in normal and in transformed cells...
  49. doi request reprint Comparative analysis of the molecular mechanisms controlling the initiation of chromosomal DNA replication in yeast and in mammalian cells
    Elena Sacco
    Department of Biotechnology and Biosciences, University of Milano Bicocca, Milano, Italy
    Biotechnol Adv 30:73-98. 2012
    ..We conclude that a systems biology approach, integrating molecular analysis with modeling and computational investigations, is the best choice to investigate the control of DNA replication in mammalian cells...
  50. doi request reprint Targeting reactive astrogliosis by novel biotechnological strategies
    Anna Maria Colangelo
    Department of Biotechnology and Biosciences, University of Milano Bicocca, Milano, Italy
    Biotechnol Adv 30:261-71. 2012
    ....
  51. ncbi request reprint Mutations in the "lid" region affect chain length specificity and thermostability of a Pseudomonas fragi lipase
    Gianluca Santarossa
    Dipartimento di Biotecnologie e Bioscienze, Universita degli Studi di Milano Bicocca, Italy
    FEBS Lett 579:2383-6. 2005
    ..Moreover, mutations conferred to PFL higher temperature stability. On the other hand, replacement of the serine at position 141 by glycine destabilized the protein...
  52. ncbi request reprint Glucose metabolism and cell size in continuous cultures of Saccharomyces cerevisiae
    Danilo Porro
    Dipartimento di Biotecnologie e Bioscienze, Universita degli Studi di Milano Bicocca, P zza della Scienza 2, 20126 Milan, Italy
    FEMS Microbiol Lett 229:165-71. 2003
    ..These results indicate a link between glucose metabolism and cell size in budding yeast, as observed for mammalian cells...
  53. ncbi request reprint The yeast Zygosaccharomyces bailii: a new host for heterologous protein production, secretion and for metabolic engineering applications
    Paola Branduardi
    Dipartimento di Biotecnologie e Bioscienze, Universita degli Studi di Milano Bicocca, Piazza della Scienza 2, 20126 Milan, Italy
    FEMS Yeast Res 4:493-504. 2004
    ....
  54. doi request reprint A new nerve growth factor-mimetic peptide active on neuropathic pain in rats
    Anna Maria Colangelo
    Laboratorio di Neuroscienze R Levi Montalcini and Dipartimento di Biotecnologie e Bioscienze, Universita di Milano Bicocca, 20126 Milan, Italy
    J Neurosci 28:2698-709. 2008
    ....
  55. pmc Sequence of the lid affects activity and specificity of Candida rugosa lipase isoenzymes
    Stefania Brocca
    Dipartimento di Biotecnologie e Bioscienze, Universita degli Studi di Milano Bicocca, 20126 Milano, Italy
    Protein Sci 12:2312-9. 2003
    ..On the other hand, a specific shift in the chain-length specificity was not observed. Chimeric proteins displayed different sensitivity to detergents in the reaction medium...
  56. ncbi request reprint Different types of cell death in organismal aging and longevity: state of the art and possible systems biology approach
    Stefano Salvioli
    Department of Experimental Pathology, University of Bologna, Via S Giacomo 12, 40126 Bologna, Italy
    Curr Pharm Des 14:226-36. 2008
    ..Since the "decisional process" that leads the cell to death is very complex, we will also discuss the possibility to address this topic with a systems biology approach...