Stefania Abbruzzetti

Summary

Affiliation: University of Parma
Country: Italy

Publications

  1. doi request reprint Oxygen and nitric oxide rebinding kinetics in nonsymbiotic hemoglobin AHb1 from Arabidopsis thaliana
    Stefania Abbruzzetti
    Dipartimento di Fisica, Universita degli Studi di Parma, Parma, Italy
    IUBMB Life 63:1094-100. 2011
  2. ncbi request reprint Different roles of protein dynamics and ligand migration in non-symbiotic hemoglobins AHb1 and AHb2 from Arabidopsis thaliana
    Stefano Bruno
    Dipartimento di Biochimica e Biologia Molecolare, Universita degli Studi di Parma, Italy
    Gene 398:224-33. 2007
  3. ncbi request reprint Ligand migration in nonsymbiotic hemoglobin AHb1 from Arabidopsis thaliana
    Stefania Abbruzzetti
    Dipartimento di Fisica, Universita degli Studi di Parma, NEST CNR INFM, Parma, Italy
    J Phys Chem B 111:12582-90. 2007
  4. doi request reprint Structural plasticity and functional implications of internal cavities in distal mutants of type 1 non-symbiotic hemoglobin AHb1 from Arabidopsis thaliana
    Serena Faggiano
    Dipartimento di Biochimica e Biologia Molecolare, Universita degli Studi di Parma, Parma, Italy
    J Phys Chem B 113:16028-38. 2009
  5. ncbi request reprint The reactivity with CO of AHb1 and AHb2 from Arabidopsis thaliana is controlled by the distal HisE7 and internal hydrophobic cavities
    Stefano Bruno
    Dipartimento di Biochimica e Biologia Molecolare, Universita degli Studi di Parma, Parma, Italy
    J Am Chem Soc 129:2880-9. 2007
  6. doi request reprint Histidine E7 dynamics modulates ligand exchange between distal pocket and solvent in AHb1 from Arabidopsis thaliana
    Francesca Spyrakis
    Dipartimento di Chimica Generale ed Inorganica, Chimica Analitica, Chimica Fisica, Universita degli Studi di Parma, Italy
    J Phys Chem B 115:4138-46. 2011
  7. doi request reprint Oxygen binding to Arabidopsis thaliana AHb2 nonsymbiotic hemoglobin: evidence for a role in oxygen transport
    Francesca Spyrakis
    Dipartimento di Chimica Generale ed Inorganica, Chimica Analitica, Chimica Fisica, Universita degli Studi di Parma, Italy
    IUBMB Life 63:355-62. 2011
  8. doi request reprint Characterization of ligand migration mechanisms inside hemoglobins from the analysis of geminate rebinding kinetics
    Stefania Abbruzzetti
    Dipartimento di Fisica Università degli Studi di Parma, Parma, Italy
    Methods Enzymol 437:329-45. 2008
  9. pmc Ligand migration through the internal hydrophobic cavities in human neuroglobin
    Stefania Abbruzzetti
    Dipartimento di Fisica, National Enterprise for nanoScience and nanoTechnology CNR INFM, Universita degli Studi di Parma, 43100 Parma, Italy
    Proc Natl Acad Sci U S A 106:18984-9. 2009
  10. ncbi request reprint Time-resolved methods in Biophysics. 2. Monitoring haem proteins at work with nanosecond laser flash photolysis
    Stefania Abbruzzetti
    Dipartimento di Fisica, Universita degli Studi di Parma, Parma, Italy
    Photochem Photobiol Sci 5:1109-20. 2006

Detail Information

Publications24

  1. doi request reprint Oxygen and nitric oxide rebinding kinetics in nonsymbiotic hemoglobin AHb1 from Arabidopsis thaliana
    Stefania Abbruzzetti
    Dipartimento di Fisica, Universita degli Studi di Parma, Parma, Italy
    IUBMB Life 63:1094-100. 2011
    ..However, the accessibility to this site seems to be strongly ligand-dependent, being lower for O2 and higher for NO...
  2. ncbi request reprint Different roles of protein dynamics and ligand migration in non-symbiotic hemoglobins AHb1 and AHb2 from Arabidopsis thaliana
    Stefano Bruno
    Dipartimento di Biochimica e Biologia Molecolare, Universita degli Studi di Parma, Italy
    Gene 398:224-33. 2007
    ..This finding strongly supports the idea that they have distinct physiological functions...
  3. ncbi request reprint Ligand migration in nonsymbiotic hemoglobin AHb1 from Arabidopsis thaliana
    Stefania Abbruzzetti
    Dipartimento di Fisica, Universita degli Studi di Parma, NEST CNR INFM, Parma, Italy
    J Phys Chem B 111:12582-90. 2007
    ..This finding may be of relevance to the physiological NO dioxygenase activity of this protein, which requires sequential binding of two substrates, NO and O2, to the heme...
  4. doi request reprint Structural plasticity and functional implications of internal cavities in distal mutants of type 1 non-symbiotic hemoglobin AHb1 from Arabidopsis thaliana
    Serena Faggiano
    Dipartimento di Biochimica e Biologia Molecolare, Universita degli Studi di Parma, Parma, Italy
    J Phys Chem B 113:16028-38. 2009
    ..Overall, data are consistent with the putative NO-dioxygenase activity attributed to AHb1...
  5. ncbi request reprint The reactivity with CO of AHb1 and AHb2 from Arabidopsis thaliana is controlled by the distal HisE7 and internal hydrophobic cavities
    Stefano Bruno
    Dipartimento di Biochimica e Biologia Molecolare, Universita degli Studi di Parma, Parma, Italy
    J Am Chem Soc 129:2880-9. 2007
    ..Overall, these kinetic and structural data are consistent with the putative NO-dioxygenase activity previously attributed to AHb1, whereas the role of AHb2 remains elusive...
  6. doi request reprint Histidine E7 dynamics modulates ligand exchange between distal pocket and solvent in AHb1 from Arabidopsis thaliana
    Francesca Spyrakis
    Dipartimento di Chimica Generale ed Inorganica, Chimica Analitica, Chimica Fisica, Universita degli Studi di Parma, Italy
    J Phys Chem B 115:4138-46. 2011
    ..Overall, these findings provide evidence supporting the functional implications of the conformational rearrangement found for the distal His in AHb1, which mimics the gating role proposed for the same residue in myoglobin...
  7. doi request reprint Oxygen binding to Arabidopsis thaliana AHb2 nonsymbiotic hemoglobin: evidence for a role in oxygen transport
    Francesca Spyrakis
    Dipartimento di Chimica Generale ed Inorganica, Chimica Analitica, Chimica Fisica, Universita degli Studi di Parma, Italy
    IUBMB Life 63:355-62. 2011
    ..Overall, our results are consistent with a role for AHb2 as an oxygen carrier, as recently proposed on the basis of experiments on AHb2-overexpressing mutants of A. thaliana...
  8. doi request reprint Characterization of ligand migration mechanisms inside hemoglobins from the analysis of geminate rebinding kinetics
    Stefania Abbruzzetti
    Dipartimento di Fisica Università degli Studi di Parma, Parma, Italy
    Methods Enzymol 437:329-45. 2008
    ....
  9. pmc Ligand migration through the internal hydrophobic cavities in human neuroglobin
    Stefania Abbruzzetti
    Dipartimento di Fisica, National Enterprise for nanoScience and nanoTechnology CNR INFM, Universita degli Studi di Parma, 43100 Parma, Italy
    Proc Natl Acad Sci U S A 106:18984-9. 2009
    ..Overall, data are consistent with the presence of a discrete system of docking sites, possibly acting as reservoirs for the putative cosubstrates and for other reactive species involved in the physiologically relevant reaction...
  10. ncbi request reprint Time-resolved methods in Biophysics. 2. Monitoring haem proteins at work with nanosecond laser flash photolysis
    Stefania Abbruzzetti
    Dipartimento di Fisica, Universita degli Studi di Parma, Parma, Italy
    Photochem Photobiol Sci 5:1109-20. 2006
    ..The comparison between time courses recorded on haemoglobins in solution and encapsulated in silica gels allows for the highlighting of different interplays between protein dynamics and ligand migration...
  11. doi request reprint Ligand migration and hexacoordination in type 1 non-symbiotic rice hemoglobin
    Nitin Kumar Bisht
    Dipartimento di Fisica, Universita degli Studi di Parma, Parma, Italy
    Biochim Biophys Acta 1814:1042-53. 2011
    ..This article is part of a Special Issue entitled: Protein Dynamics: Experimental and Computational Approaches...
  12. pmc Ligation tunes protein reactivity in an ancient haemoglobin: kinetic evidence for an allosteric mechanism in Methanosarcina acetivorans protoglobin
    Stefania Abbruzzetti
    Dipartimento di Fisica, Universita degli Studi di Parma, Parma, Italy
    PLoS ONE 7:e33614. 2012
    ..The equilibrium is shifted towards the species with low binding (and high dissociation) rates for the unliganded molecules. A quantitative model is proposed to describe the observed carbonylation kinetics...
  13. ncbi request reprint Determination of microscopic rate constants for CO binding and migration in myoglobin encapsulated in silica gels
    Silvia Sottini
    Dipartimento di Fisica, Universita degli Studi di Parma, Parco Area delle Scienze 7 A, Universita degli Studi di Parma, Parco Area delle Scienze 23 A, 43100 Parma, Italy
    J Phys Chem B 109:19523-8. 2005
    ..Microscopic rates and activation barriers were estimated using a four-state model...
  14. pmc Low affinity PEGylated hemoglobin from Trematomus bernacchii, a model for hemoglobin-based blood substitutes
    Daniela Coppola
    Department of Biochemistry and Molecular Biology, University of Parma, Parma, Italy
    BMC Biochem 12:66. 2011
    ..These recent findings call for the biotechnological development of stable, low-affinity PEGylated hemoglobins with low NO dioxygenase reactivity...
  15. ncbi request reprint Geminate rebinding in R-state hemoglobin: kinetic and computational evidence for multiple hydrophobic pockets
    Silvia Sottini
    Dipartimento di Fisica, Universita degli Studi di Parma, Parco Area delle Scienze 7 A, 43100 Parma, Italy
    J Am Chem Soc 127:17427-32. 2005
    ..Furthermore, the connection between the two xenon sites is relatively open, explaining why the decreased mobility of the protein with viscosity only slightly perturbs the energetics of ligand migration between the two sites...
  16. doi request reprint Ligand reactivity and allosteric regulation of hemoglobin-based oxygen carriers
    Luca Ronda
    Department of Biochemistry and Molecular Biology, University of Parma, Parma, Italy
    Biochim Biophys Acta 1784:1365-77. 2008
    ....
  17. doi request reprint Ligand migration through hemeprotein cavities: insights from laser flash photolysis and molecular dynamics simulations
    Stefania Abbruzzetti
    Dipartimento di Fisica e Scienze della Terra, Universita degli Studi di Parma, viale delle Scienze 7A, 43124, Parma, Italy
    Phys Chem Chem Phys 15:10686-701. 2013
    ....
  18. ncbi request reprint Acid-induced unfolding of myoglobin triggered by a laser pH jump method
    Stefania Abbruzzetti
    Dipartimento di Fisica, Universita degli Studi di Parma, Parco Area delle Scienze 7 A, 43100 Parma, Italy
    Photochem Photobiol Sci 5:621-8. 2006
    ..The complete time course of the unfolding kinetics, spanning a range from milliseconds to several seconds, could be accurately reproduced by monitoring absorbance changes in the visible spectrum at 633 nm...
  19. ncbi request reprint Molten globule formation in apomyoglobin monitored by the fluorescent probe Nile Red
    Eugenia Polverini
    Dipartimento di Fisica, Universita degli Studi di Parma, Viale G P Usberti 7 a, 43100 Parma, Italy
    Biochemistry 45:5111-21. 2006
    ..Under these conditions, NR is positioned in a different orientation, reaching a more hydrophobic environment in agreement with the spectroscopic data...
  20. ncbi request reprint Kinetics of histidine dissociation from the heme Fe(III) in N-fragment (residues 1-56) of cytochrome c
    Stefania Abbruzzetti
    Dipartimento di Fisica, Universita degli Studi di Parma, Parco Area delle Scienze 7 A 43100 Parma, Italy
    Protein J 23:519-27. 2004
    ..This underlines the role played by the missing segment (residues 57-104) peptide chain in discriminating deligation of the "nonnative" His from the sixth coordination position of the metal...
  21. doi request reprint Heterogeneous kinetics of the carbon monoxide association and dissociation reaction to nitrophorin 4 and 7 coincide with structural heterogeneity of the gate-loop
    Stefania Abbruzzetti
    Dipartimento di Fisica, Universita degli Studi di Parma, viale delle Scienze 7A, I 43124, Parma, Italy
    J Am Chem Soc 134:9986-98. 2012
    ....
  22. doi request reprint Photoswitching of E222Q GFP mutants: "concerted" mechanism of chromophore isomerization and protonation
    Stefania Abbruzzetti
    Dipartimento di Fisica, Universita di Parma, Viale G P Usberti 7a, 43100, Parma, Italy
    Photochem Photobiol Sci 9:1307-19. 2010
    ..Such a "concerted" mechanism is, in our opinion, at the basis of efficient photochromic behavior and might be activated by the E222Q mutation...
  23. ncbi request reprint Kinetics of acid-induced spectral changes in the GFPmut2 chromophore
    Stefania Abbruzzetti
    Dipartimento di Fisica, Universita di Parma, Parco Area delle Scienze 7 A, 43100 Parma, Italy
    J Am Chem Soc 127:626-35. 2005
    ..A kinetic model suggests that, in addition to protecting the chromophore from the solvent, His148 may act as the primary acceptor for the protons on the way to the chromophore...
  24. doi request reprint A photochromic bacterial photoreceptor with potential for super-resolution microscopy
    Aba Losi
    Dipartimento di Fisica, Universita di Parma, viale delle Scienze 7A, 43124 Parma, Italy
    Photochem Photobiol Sci 12:231-5. 2013
    ..We demonstrate Fluorescence PhotoActivation Localization Microscopy (FPALM) studies of live Escherichia coli cells, expressing YtvA molecules...