Diego L Medina
Affiliation: Telethon Institute of Genetics and Medicine
Di Paola S, Medina D. TRPML1-/TFEB-Dependent Regulation of Lysosomal Exocytosis. Methods Mol Biol. 2019;1925:143-144 pubmed publisher
..Here, we described methods to determine TFEB activation and lysosomal exocytosis that may represent innovative tools to study lysosomal function and to develop novel therapeutic approaches to promote clearance in LSDs. ..
Di Paola S, Medina D. Ca2+-Dependent Regulation of TFEB and Lysosomal Function. Methods Mol Biol. 2019;1925:145-155 pubmed publisher
..In the case report, we described two studies where these methodologies allowed us to unravel the role of calcineurin in the dephosphorylation of TFEB as well as the involvement of TFEB in lysosomal exocytosis, respectively. ..
Maddalena A, Dell Aquila F, Giovannelli P, Tiberi P, Wanderlingh L, Montefusco S, et al
. High-throughput screening identifies kinase inhibitors that increase dual AAV vectors transduction <i>in vitro</i> and in mouse retina. Hum Gene Ther. 2018;: pubmed publisher
..Our study identifies kinase inhibitors that increase dual and single AAV transduction by modulating AAV entry and post-entry steps. ..
Gambardella G, Peluso I, Montefusco S, Bansal M, Medina D, Lawrence N, et al
. A reverse-engineering approach to dissect post-translational modulators of transcription factor's activity from transcriptional data. BMC Bioinformatics. 2015;16:279 pubmed publisher
..An on-line web-tool enabling the user to use DMI to identify post-transcriptional modulators of a transcription factor of interest che be found at http://dmi.tigem.it. ..
Di Paola S, Scotto Rosato A, Medina D. TRPML1: The Ca(2+)retaker of the lysosome. Cell Calcium. 2018;69:112-121 pubmed publisher
..Moreover, we will also discuss new potential therapeutic approaches for MLIV and LSDs based on the modulation of TRPML1-mediated signaling. ..