Fiorenzo Stirpe

Summary

Country: Italy

Publications

  1. ncbi Characterization of highly toxic type 2 ribosome-inactivating proteins from Adenia lanceolata and Adenia stenodactyla (Passifloraceae)
    Fiorenzo Stirpe
    Dipartimento di Patologia Sperimentale, Universita di Bologna, I 40126 Bologna, Italy
    Toxicon 50:94-105. 2007
  2. doi Ribosome-inactivating proteins: from toxins to useful proteins
    Fiorenzo Stirpe
    Alma Mater Studiorum Universita di Bologna, Via S Petronio Vecchio 19, I 40125 Bologna, Italy
    Toxicon 67:12-6. 2013
  3. ncbi Ribosome-inactivating proteins in edible plants and purification and characterization of a new ribosome-inactivating protein from Cucurbita moschata
    Luigi Barbieri
    Dipartimento di Patologia Sperimentale, Universita di Bologna, I 40126 Bologna, Italy
    Biochim Biophys Acta 1760:783-92. 2006
  4. ncbi Ribosome-inactivating proteins and other lectins from Adenia (Passifloraceae)
    Emanuele Pelosi
    Dipartimento di Patologia sperimentale dell Università di Bologna, Via S Giacomo 14, I 40126 Bologna, Italy
    Toxicon 46:658-63. 2005
  5. ncbi The 1.4 anstroms structure of dianthin 30 indicates a role of surface potential at the active site of type 1 ribosome inactivating proteins
    Simona Fermani
    Dipartimento di Chimica G Ciamician, Alma Mater Studiorum Universita di Bologna, Via Selmi 2, I 40126 Bologna, Italy
    J Struct Biol 149:204-12. 2005
  6. ncbi Enzymatic activity of toxic and non-toxic type 2 ribosome-inactivating proteins
    Luigi Barbieri
    Dipartimento di Patologia Sperimentale, Universita di Bologna, I 40126 Bologna, Italy
    FEBS Lett 563:219-22. 2004
  7. ncbi Detection of ricin and other ribosome-inactivating proteins by an immuno-polymerase chain reaction assay
    Chiara Lubelli
    Department of Experimental Pathology, University of Bologna, Via S Giacomo 14, I 40126 Bologna, Italy
    Anal Biochem 355:102-9. 2006
  8. ncbi Ribosome-inactivating proteins
    Fiorenzo Stirpe
    Dipartimento di Patologia Sperimentale, Universita di Bologna, I 40125 Bologna, Italy
    Toxicon 44:371-83. 2004
  9. ncbi In vitro and in vivo toxicity of type 2 ribosome-inactivating proteins lanceolin and stenodactylin on glial and neuronal cells
    Barbara Monti
    Department of Experimental Evolutionary Biology, Alma Mater Studiorum University of Bologna, Via Selmi 3, I 40126 Bologna, Italy
    Neurotoxicology 28:637-44. 2007
  10. ncbi Cloning and expression of cDNA coding for bouganin
    Marcel T den Hartog
    Tanox Pharma B V, Amsterdam, the Netherlands Dipartimento di Patologia Sperimentale, Universita di Bologna, Bologna, Italy
    Eur J Biochem 269:1772-9. 2002

Collaborators

Detail Information

Publications15

  1. ncbi Characterization of highly toxic type 2 ribosome-inactivating proteins from Adenia lanceolata and Adenia stenodactyla (Passifloraceae)
    Fiorenzo Stirpe
    Dipartimento di Patologia Sperimentale, Universita di Bologna, I 40126 Bologna, Italy
    Toxicon 50:94-105. 2007
    ..16 microg/kg (lanceolin) and 2.76 microg/kg (stenodactylin) at 48 h. Thus, lanceolin and stenodactylin have all the properties of the toxic type 2 ribosome-inactivating proteins and are amongst the most potent toxins of plant origin...
  2. doi Ribosome-inactivating proteins: from toxins to useful proteins
    Fiorenzo Stirpe
    Alma Mater Studiorum Universita di Bologna, Via S Petronio Vecchio 19, I 40125 Bologna, Italy
    Toxicon 67:12-6. 2013
    ..In agriculture, it has been observed that an enhanced expression of RIPs confers to plants an increased resistance to viruses, fungi, insects, and also to drought and salinity...
  3. ncbi Ribosome-inactivating proteins in edible plants and purification and characterization of a new ribosome-inactivating protein from Cucurbita moschata
    Luigi Barbieri
    Dipartimento di Patologia Sperimentale, Universita di Bologna, I 40126 Bologna, Italy
    Biochim Biophys Acta 1760:783-92. 2006
    ..The C. moschata RIP gave a weak cross-reaction only with an antiserum against dianthin 32, but not with antisera against other RIPs, and had superoxide dismutase, antifungal and antibacterial activities...
  4. ncbi Ribosome-inactivating proteins and other lectins from Adenia (Passifloraceae)
    Emanuele Pelosi
    Dipartimento di Patologia sperimentale dell Università di Bologna, Via S Giacomo 14, I 40126 Bologna, Italy
    Toxicon 46:658-63. 2005
    ..lanceolata and A. stenodactyla had the highest cytotoxicity, inhibiting cell protein synthesis with IC50s (concentration inhibiting by 50%) below 0.1 ng/ml, and deadenylate DNA, thus being type 2 ribosome-inactivating proteins...
  5. ncbi The 1.4 anstroms structure of dianthin 30 indicates a role of surface potential at the active site of type 1 ribosome inactivating proteins
    Simona Fermani
    Dipartimento di Chimica G Ciamician, Alma Mater Studiorum Universita di Bologna, Via Selmi 2, I 40126 Bologna, Italy
    J Struct Biol 149:204-12. 2005
    ..The possible relationship between the charge distribution and the behavior of the proteins toward different substrates is discussed...
  6. ncbi Enzymatic activity of toxic and non-toxic type 2 ribosome-inactivating proteins
    Luigi Barbieri
    Dipartimento di Patologia Sperimentale, Universita di Bologna, I 40126 Bologna, Italy
    FEBS Lett 563:219-22. 2004
    ..All type 2 RIPs differ in their depurinating activity on the different substrates with differences of up to one to two logs. The toxicity of type 2 RIPs is independent of their enzymatic activity on nucleic acids or on ribosomes...
  7. ncbi Detection of ricin and other ribosome-inactivating proteins by an immuno-polymerase chain reaction assay
    Chiara Lubelli
    Department of Experimental Pathology, University of Bologna, Via S Giacomo 14, I 40126 Bologna, Italy
    Anal Biochem 355:102-9. 2006
    ..The possibility to detect ricin in human serum was also investigated, and a similar sensitivity was observed (10 fg/ml). IPCR appears to be the most sensitive method for the detection of ricin and other RIPs...
  8. ncbi Ribosome-inactivating proteins
    Fiorenzo Stirpe
    Dipartimento di Patologia Sperimentale, Universita di Bologna, I 40125 Bologna, Italy
    Toxicon 44:371-83. 2004
    ..A pattern appears of a still largely unexplored subject, whose role in nature is probably important, and not limited to the biology of plants, since RIPs have been found also in other organisms...
  9. ncbi In vitro and in vivo toxicity of type 2 ribosome-inactivating proteins lanceolin and stenodactylin on glial and neuronal cells
    Barbara Monti
    Department of Experimental Evolutionary Biology, Alma Mater Studiorum University of Bologna, Via Selmi 3, I 40126 Bologna, Italy
    Neurotoxicology 28:637-44. 2007
    ..The retrograde transport of RIPs along neurons allows to perform experiments of target-selective lesioning, and can be exploited also to perform specific experiments of immunolesioning of selected neuronal populations...
  10. ncbi Cloning and expression of cDNA coding for bouganin
    Marcel T den Hartog
    Tanox Pharma B V, Amsterdam, the Netherlands Dipartimento di Patologia Sperimentale, Universita di Bologna, Bologna, Italy
    Eur J Biochem 269:1772-9. 2002
    ..The recombinant molecule had similar activity in a cell-free protein synthesis assay and had comparable toxicity on living cells as compared to the isolated native bouganin...
  11. ncbi Ribosome-inactivating and adenine polynucleotide glycosylase activities in Mirabilis jalapa L. tissues
    Andrea Bolognesi
    Dipartimento di Patologia Sperimentale, Universita di Bologna, Via San Giacomo 14, I 40126 Bologna, Italy
    J Biol Chem 277:13709-16. 2002
    ..Finally, MAP does not cross-react immunologically with other commonly utilized RIPs...
  12. ncbi Crystallization and preliminary X-ray diffraction analysis of two ribosome-inactivating proteins: lychnin and dianthin 30
    Simona Fermani
    Dipartimento di Chimica G Ciamician, Via Selmi 2, Alma Mater Studiorum Universita di Bologna, I 40126 Bologna, Italy
    Acta Crystallogr D Biol Crystallogr 59:1227-9. 2003
    ..The structure determination of lychnin, the sequence of which is not yet available, is in progress using the coordinates of other RIPs as models for molecular replacement...
  13. ncbi Damage to nuclear DNA induced by Shiga toxin 1 and ricin in human endothelial cells
    Maurizio Brigotti
    Dipartimento di Patologia Sperimentale, Universita degli Studi di Bologna, Italy
    FASEB J 16:365-72. 2002
    ..The results unequivocally indicate that RIPs can damage nuclear DNA in whole cells by means that are not secondary to ribosome inactivation or apoptosis...
  14. ncbi Ribosome-inactivating proteins depurinate poly(ADP-ribosyl)ated poly(ADP-ribose) polymerase and have transforming activity for 3T3 fibroblasts
    Luigi Barbieri
    Dipartimento di Patologia Sperimentale, Universita di Bologna, Via San Giacomo 14, I 40126 Bologna, Italy
    FEBS Lett 538:178-82. 2003
    ..We observed also that all RIPs but gelonin induce transformation of fibroblasts, possibly as a consequence of damage to DNA and of the altered DNA repair system...
  15. ncbi Twenty years of hunting for proteins with Carlo Alfonso Rossi
    Fiorenzo Stirpe
    Dipartimento di Patologia Sperimentale, Universita di Bologna
    Ital J Biochem 53:164-8. 2004
    ..Several lectins and toxic and non-toxic ribosome-inactivating proteins were identified, purified and characterized. The general properties of the proteins are described...